AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040923.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040923.01.T01 MTR_7g101130 96.535 202 7 0 1 202 32 233 3.87e-138 395
MsG0780040923.01.T01 MTR_1g064690 49.524 210 92 6 1 200 34 239 1.41e-56 187
MsG0780040923.01.T01 MTR_8g089585 28.846 156 106 2 41 192 75 229 2.42e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040923.01.T01 AT3G53400 41.096 219 112 6 1 203 32 249 6.37e-39 140
MsG0780040923.01.T01 AT5G03190 41.848 184 96 6 25 204 55 231 1.65e-33 125
MsG0780040923.01.T01 AT5G03190 41.848 184 96 6 25 204 79 255 2.06e-33 125
MsG0780040923.01.T01 AT1G24480 29.221 154 104 3 43 192 65 217 9.58e-14 68.6

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTTATCAAACTATGATT+TGG 0.264575 7:+84320835 MsG0780040923.01.T01:CDS
GATTCGATTCTGAAAGAAAT+AGG 0.318467 7:-84320924 None:intergenic
ATTTCTTTCAGAATCGAATC+TGG 0.324960 7:+84320927 MsG0780040923.01.T01:CDS
GAATGTGGTCACTGAGTTAA+TGG 0.342946 7:+84320988 MsG0780040923.01.T01:CDS
GTTCTTAAGCCTAATGGAAT+TGG 0.390560 7:+84321259 MsG0780040923.01.T01:CDS
CCCTGCTTTGCTTGTTCTTG+AGG 0.393326 7:+84321228 MsG0780040923.01.T01:CDS
CATTTGATTTCGTATTGTCT+AGG 0.400138 7:+84321185 MsG0780040923.01.T01:CDS
GATCTCAACAATGATGAAAC+TGG 0.404198 7:-84321340 None:intergenic
AACGATATTCTTCATCTTGA+GGG 0.413335 7:-84321137 None:intergenic
GAGCGTGTTCTTAAGCCTAA+TGG 0.420052 7:+84321253 MsG0780040923.01.T01:CDS
TGTTGGTGAAGGTTCTTCAA+TGG 0.432990 7:+84321048 MsG0780040923.01.T01:CDS
ATTGTCTAGGGATCTTGATA+AGG 0.440951 7:+84321198 MsG0780040923.01.T01:CDS
ATTTGATTTCGTATTGTCTA+GGG 0.451800 7:+84321186 MsG0780040923.01.T01:CDS
ACCTCAAGAACAAGCAAAGC+AGG 0.456915 7:-84321229 None:intergenic
GTTAAAGCAATGAAACAGTT+GGG 0.467346 7:+84321073 MsG0780040923.01.T01:CDS
TTTCTTTCAGAATCGAATCT+GGG 0.479609 7:+84320928 MsG0780040923.01.T01:CDS
ATTCTGAAAGAAATAGGAAG+TGG 0.500053 7:-84320918 None:intergenic
AGAAGTGCACCAATTCCATT+AGG 0.511486 7:-84321268 None:intergenic
AAACGATATTCTTCATCTTG+AGG 0.511737 7:-84321138 None:intergenic
TGTTAAAGCAATGAAACAGT+TGG 0.517899 7:+84321072 MsG0780040923.01.T01:CDS
TTCTGAAAGAAATAGGAAGT+GGG 0.537629 7:-84320917 None:intergenic
GGTTTGGTTAATGTCAAACT+AGG 0.546298 7:-84321483 None:intergenic
AATGTGGTCACTGAGTTAAT+GGG 0.566533 7:+84320989 MsG0780040923.01.T01:CDS
TTCTTTCAGAATCGAATCTG+GGG 0.582119 7:+84320929 MsG0780040923.01.T01:CDS
AATACCCTCTGTGTTGGTGA+AGG 0.584957 7:+84321037 MsG0780040923.01.T01:CDS
CGTTAACTTGACAGTGAATG+TGG 0.591281 7:+84320973 MsG0780040923.01.T01:CDS
GTCAAACTAGGACAATCAGC+AGG 0.603229 7:-84321471 None:intergenic
TCTGAAAGAAATAGGAAGTG+GGG 0.627270 7:-84320916 None:intergenic
CCTCAAGAACAAGCAAAGCA+GGG 0.639198 7:-84321228 None:intergenic
GATCTTTCTTGAAAACAACA+AGG 0.661566 7:-84321405 None:intergenic
GAAGCGAATACCCTCTGTGT+TGG 0.663485 7:+84321031 MsG0780040923.01.T01:CDS
AGAACCTTCACCAACACAGA+GGG 0.664286 7:-84321041 None:intergenic
AAGAACCTTCACCAACACAG+AGG 0.674044 7:-84321042 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGATTTCGTATTGTCTA+GGG + Chr7:84321186-84321205 MsG0780040923.01.T01:CDS 25.0%
AAACGATATTCTTCATCTTG+AGG - Chr7:84321141-84321160 None:intergenic 30.0%
AACGATATTCTTCATCTTGA+GGG - Chr7:84321140-84321159 None:intergenic 30.0%
ATTTCTTTCAGAATCGAATC+TGG + Chr7:84320927-84320946 MsG0780040923.01.T01:CDS 30.0%
CGCTTTATCAAACTATGATT+TGG + Chr7:84320835-84320854 MsG0780040923.01.T01:CDS 30.0%
GATCTTTCTTGAAAACAACA+AGG - Chr7:84321408-84321427 None:intergenic 30.0%
GTTAAAGCAATGAAACAGTT+GGG + Chr7:84321073-84321092 MsG0780040923.01.T01:CDS 30.0%
TGTTAAAGCAATGAAACAGT+TGG + Chr7:84321072-84321091 MsG0780040923.01.T01:CDS 30.0%
TTTCTTTCAGAATCGAATCT+GGG + Chr7:84320928-84320947 MsG0780040923.01.T01:CDS 30.0%
! ATTCTGAAAGAAATAGGAAG+TGG - Chr7:84320921-84320940 None:intergenic 30.0%
! CATTTGATTTCGTATTGTCT+AGG + Chr7:84321185-84321204 MsG0780040923.01.T01:CDS 30.0%
! GATTCGATTCTGAAAGAAAT+AGG - Chr7:84320927-84320946 None:intergenic 30.0%
! TTCTGAAAGAAATAGGAAGT+GGG - Chr7:84320920-84320939 None:intergenic 30.0%
!! TTGGGTTTTTCAACTGTTAT+TGG + Chr7:84321091-84321110 MsG0780040923.01.T01:CDS 30.0%
AATGTGGTCACTGAGTTAAT+GGG + Chr7:84320989-84321008 MsG0780040923.01.T01:CDS 35.0%
ATTGTCTAGGGATCTTGATA+AGG + Chr7:84321198-84321217 MsG0780040923.01.T01:CDS 35.0%
GATCTCAACAATGATGAAAC+TGG - Chr7:84321343-84321362 None:intergenic 35.0%
GTTCTTAAGCCTAATGGAAT+TGG + Chr7:84321259-84321278 MsG0780040923.01.T01:CDS 35.0%
TCATCTTGAGGGAGAAAAAA+CGG - Chr7:84321129-84321148 None:intergenic 35.0%
TCTGAAAGAAATAGGAAGTG+GGG - Chr7:84320919-84320938 None:intergenic 35.0%
TTCTTTCAGAATCGAATCTG+GGG + Chr7:84320929-84320948 MsG0780040923.01.T01:CDS 35.0%
GAATGTGGTCACTGAGTTAA+TGG + Chr7:84320988-84321007 MsG0780040923.01.T01:CDS 40.0%
! AGAAGTGCACCAATTCCATT+AGG - Chr7:84321271-84321290 None:intergenic 40.0%
! CGTTAACTTGACAGTGAATG+TGG + Chr7:84320973-84320992 MsG0780040923.01.T01:CDS 40.0%
! TGTTGGTGAAGGTTCTTCAA+TGG + Chr7:84321048-84321067 MsG0780040923.01.T01:CDS 40.0%
AAGAACCTTCACCAACACAG+AGG - Chr7:84321045-84321064 None:intergenic 45.0%
ACCTCAAGAACAAGCAAAGC+AGG - Chr7:84321232-84321251 None:intergenic 45.0%
AGAACCTTCACCAACACAGA+GGG - Chr7:84321044-84321063 None:intergenic 45.0%
CCTCAAGAACAAGCAAAGCA+GGG - Chr7:84321231-84321250 None:intergenic 45.0%
CTTGAGGGAGAAAAAACGGT+TGG - Chr7:84321125-84321144 None:intergenic 45.0%
GAGCGTGTTCTTAAGCCTAA+TGG + Chr7:84321253-84321272 MsG0780040923.01.T01:CDS 45.0%
! AATACCCTCTGTGTTGGTGA+AGG + Chr7:84321037-84321056 MsG0780040923.01.T01:CDS 45.0%
CCCTGCTTTGCTTGTTCTTG+AGG + Chr7:84321228-84321247 MsG0780040923.01.T01:CDS 50.0%
GAAGCGAATACCCTCTGTGT+TGG + Chr7:84321031-84321050 MsG0780040923.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 84320818 84321492 84320818 ID=MsG0780040923.01;Name=MsG0780040923.01
Chr7 mRNA 84320818 84321492 84320818 ID=MsG0780040923.01.T01;Parent=MsG0780040923.01;Name=MsG0780040923.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|224
Chr7 exon 84320818 84321492 84320818 ID=MsG0780040923.01.T01:exon:40707;Parent=MsG0780040923.01.T01
Chr7 CDS 84320818 84321492 84320818 ID=MsG0780040923.01.T01:cds;Parent=MsG0780040923.01.T01
Gene Sequence

>MsG0780040923.01.T01

ATGATTGCGCTTCTTCGCGCTTTATCAAACTATGATTTGGAAACTTTTGCATCAAATTTGAACTGTTTTGCAGATTTCGAAAACAACGTCACTTCAAACCCCACTTCCTATTTCTTTCAGAATCGAATCTGGGGTTCGATCAATTGCGAAAAAGACGTTAACTTGACAGTGAATGTGGTCACTGAGTTAATGGGTAAGCAGTTTTTGAACTGTGAAGCGAATACCCTCTGTGTTGGTGAAGGTTCTTCAATGGCTGTTAAAGCAATGAAACAGTTGGGTTTTTCAACTGTTATTGGTGTTTATACCAACCGTTTTTTCTCCCTCAAGATGAAGAATATCGTTTATGAGCTAGATTATCAAGATTCTTCATTTGATTTCGTATTGTCTAGGGATCTTGATAAGGTTTCTGTCCCTGCTTTGCTTGTTCTTGAGGTTGAGCGTGTTCTTAAGCCTAATGGAATTGGTGCACTTCTTGTTGCTGCTAAAAGTTCACATCACAATGATTTGATTAGATCAGCTACACCAGTTTCATCATTGTTGAGATCTTCTAGTGTCGTTCATGTTGATTCTGTTGATGATGAACTTAACCTTGTTGTTTTCAAGAAAGATCTGAAAATGCAACAACTTTTTTCAATCATAATCAGTATAGTTTGCCTGCTGATTGTCCTAGTTTGA
Protein sequence

>MsG0780040923.01.T01

MIALLRALSNYDLETFASNLNCFADFENNVTSNPTSYFFQNRIWGSINCEKDVNLTVNVVTELMGKQFLNCEANTLCVGEGSSMAVKAMKQLGFSTVIGVYTNRFFSLKMKNIVYELDYQDSSFDFVLSRDLDKVSVPALLVLEVERVLKPNGIGALLVAAKSSHHNDLIRSATPVSSLLRSSSVVHVDSVDDELNLVVFKKDLKMQQLFSIIISIVCLLIVLV*