Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040924.01.T01 | XP_003625620.1 | 96.257 | 187 | 7 | 0 | 2 | 188 | 279 | 465 | 1.47E-127 | 378 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040924.01.T01 | Q2HV37 | 96.257 | 187 | 7 | 0 | 2 | 188 | 279 | 465 | 7.00e-128 | 378 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003427.01 | MsG0780040924.01 | 0.807984 | 3.870568e-50 | 2.186729e-47 |
| MsG0780040923.01 | MsG0780040924.01 | 0.849496 | 3.115111e-60 | 5.916394e-57 |
| MsG0780040924.01 | MsG0780041063.01 | 0.810913 | 9.097997e-51 | 5.554495e-48 |
| MsG0780040924.01 | MsG0880042773.01 | 0.811357 | 7.289243e-51 | 4.502200e-48 |
| MsG0780040924.01 | MsG0880043637.01 | 0.832914 | 7.201178e-56 | 8.163822e-53 |
| MsG0780040924.01 | MsG0880045890.01 | 0.830107 | 3.538456e-55 | 3.692355e-52 |
| MsG0480020463.01 | MsG0780040924.01 | 0.810307 | 1.229960e-50 | 7.388269e-48 |
| MsG0480020847.01 | MsG0780040924.01 | 0.803297 | 3.728870e-49 | 1.867004e-46 |
| MsG0480022998.01 | MsG0780040924.01 | 0.839959 | 1.159449e-57 | 1.627301e-54 |
| MsG0380016703.01 | MsG0780040924.01 | 0.819186 | 1.325500e-52 | 1.012862e-49 |
| MsG0380016920.01 | MsG0780040924.01 | 0.821744 | 3.430840e-53 | 2.815210e-50 |
| MsG0280009783.01 | MsG0780040924.01 | 0.806667 | 7.359428e-50 | 4.017681e-47 |
| MsG0280010352.01 | MsG0780040924.01 | 0.806241 | 9.049436e-50 | 4.887386e-47 |
| MsG0780035954.01 | MsG0780040924.01 | 0.839136 | 1.897994e-57 | 2.596686e-54 |
| MsG0780038332.01 | MsG0780040924.01 | 0.807011 | 6.225416e-50 | 3.429133e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040924.01.T01 | MTR_7g101130 | 96.257 | 187 | 7 | 0 | 2 | 188 | 279 | 465 | 1.77e-131 | 378 |
| MsG0780040924.01.T01 | MTR_1g064690 | 55.276 | 199 | 70 | 7 | 5 | 199 | 294 | 477 | 5.99e-63 | 202 |
| MsG0780040924.01.T01 | MTR_7g070530 | 31.308 | 214 | 117 | 7 | 3 | 187 | 295 | 507 | 1.58e-26 | 106 |
| MsG0780040924.01.T01 | MTR_4g013290 | 29.245 | 212 | 120 | 5 | 5 | 187 | 297 | 507 | 1.44e-25 | 103 |
| MsG0780040924.01.T01 | MTR_3g031200 | 29.167 | 216 | 114 | 6 | 5 | 187 | 283 | 492 | 1.77e-23 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040924.01.T01 | AT3G53400 | 58.602 | 186 | 62 | 4 | 5 | 190 | 295 | 465 | 2.55e-68 | 216 |
| MsG0780040924.01.T01 | AT5G03190 | 53.158 | 190 | 77 | 2 | 2 | 190 | 272 | 450 | 5.88e-66 | 210 |
| MsG0780040924.01.T01 | AT5G03190 | 53.158 | 190 | 77 | 2 | 2 | 190 | 296 | 474 | 9.83e-66 | 210 |
| MsG0780040924.01.T01 | AT5G01710 | 29.493 | 217 | 116 | 6 | 5 | 187 | 298 | 511 | 3.34e-25 | 102 |
Find 46 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTAGCCTTTGGTGATTA+TGG | 0.267469 | 7:+84322145 | MsG0780040924.01.T01:CDS |
| GGTTGGTTTATATTGATATT+GGG | 0.306403 | 7:+84321600 | MsG0780040924.01.T01:CDS |
| AGGTTGGTTTATATTGATAT+TGG | 0.318319 | 7:+84321599 | MsG0780040924.01.T01:CDS |
| TTGAAAGCTTTCTGATCAAT+AGG | 0.326104 | 7:-84321674 | None:intergenic |
| AACCTTAGGTGTTTAGCCTT+TGG | 0.379173 | 7:+84322136 | MsG0780040924.01.T01:CDS |
| ATCACGTTTGTTTATCATCC+TGG | 0.389501 | 7:+84321755 | MsG0780040924.01.T01:CDS |
| TTCCTTGTTTGTCAAGGCAT+TGG | 0.407341 | 7:-84322029 | None:intergenic |
| TTATCACCATCTTCTTTCTC+AGG | 0.407873 | 7:-84322004 | None:intergenic |
| AGCCTCAGCAGCGACTTTAC+CGG | 0.421933 | 7:-84321783 | None:intergenic |
| TGGTGTGTATGTGCATCAAT+TGG | 0.449898 | 7:+84322087 | MsG0780040924.01.T01:CDS |
| AACTCTTCCTCCCCAACAAA+AGG | 0.457530 | 7:-84321833 | None:intergenic |
| GGTGTGTATGTGCATCAATT+GGG | 0.459474 | 7:+84322088 | MsG0780040924.01.T01:CDS |
| AAGAGTTTGATTTCCTTGCC+TGG | 0.462393 | 7:+84321849 | MsG0780040924.01.T01:CDS |
| AACTTAGGCAAATATGAAAC+CGG | 0.474561 | 7:-84321554 | None:intergenic |
| GTTTATCATCCTGGATTAGC+CGG | 0.485999 | 7:+84321764 | MsG0780040924.01.T01:CDS |
| AAGGCTAAACACCTAAGGTT+TGG | 0.490858 | 7:-84322133 | None:intergenic |
| TGAATGCAAATGTTAGTGAT+TGG | 0.490906 | 7:+84321636 | MsG0780040924.01.T01:CDS |
| GTTGTACTCAAGATGAATGC+AGG | 0.496244 | 7:+84321902 | MsG0780040924.01.T01:CDS |
| TATGAAACCGGCATCGGCAT+CGG | 0.497449 | 7:-84321542 | None:intergenic |
| TTCTGATCAATAGGATATGA+AGG | 0.501367 | 7:-84321665 | None:intergenic |
| CAGTTTCTTTGAACCAGGCA+AGG | 0.506755 | 7:-84321862 | None:intergenic |
| GTTGGTTTATATTGATATTG+GGG | 0.508956 | 7:+84321601 | MsG0780040924.01.T01:CDS |
| TAATGTCGGAGACGGAGAGC+TGG | 0.513267 | 7:+84321808 | MsG0780040924.01.T01:CDS |
| GTTTATATTGATATTGGGGT+AGG | 0.516469 | 7:+84321605 | MsG0780040924.01.T01:CDS |
| GCGACTTTACCGGCTAATCC+AGG | 0.522388 | 7:-84321773 | None:intergenic |
| TGTTTCTACTAGGAAGAGGT+TGG | 0.531207 | 7:+84321583 | MsG0780040924.01.T01:CDS |
| AGTTTGTAGATGTTTCTACT+AGG | 0.532167 | 7:+84321573 | MsG0780040924.01.T01:CDS |
| GGCAAATATGAAACCGGCAT+CGG | 0.532678 | 7:-84321548 | None:intergenic |
| TAGATGTTTCTACTAGGAAG+AGG | 0.535930 | 7:+84321579 | MsG0780040924.01.T01:CDS |
| TTGCACAGTTTCTTTGAACC+AGG | 0.540355 | 7:-84321867 | None:intergenic |
| GTGAAAACCGATGCCGATGC+CGG | 0.547352 | 7:+84321535 | None:intergenic |
| TGCATACAAGACCAAACCTT+AGG | 0.556885 | 7:+84322122 | MsG0780040924.01.T01:CDS |
| CACCAAAGGCTAAACACCTA+AGG | 0.557644 | 7:-84322138 | None:intergenic |
| AGATGTCCTGAGAAAGAAGA+TGG | 0.561930 | 7:+84321998 | MsG0780040924.01.T01:CDS |
| GTAGAAACATCTACAAACTT+AGG | 0.572228 | 7:-84321569 | None:intergenic |
| GACAAACAAGGAAAATTGCA+TGG | 0.587363 | 7:+84322039 | MsG0780040924.01.T01:CDS |
| TTGTCTTACGTGAAGAGACC+TGG | 0.587754 | 7:+84321731 | MsG0780040924.01.T01:CDS |
| TGATAAACAAACGTGATACC+AGG | 0.592660 | 7:-84321749 | None:intergenic |
| TTATCTGATGTATTTGACAG+TGG | 0.598424 | 7:+84321944 | MsG0780040924.01.T01:CDS |
| GAGGCTGATAATGTCGGAGA+CGG | 0.602304 | 7:+84321800 | MsG0780040924.01.T01:CDS |
| GCTGCTGAGGCTGATAATGT+CGG | 0.602809 | 7:+84321794 | MsG0780040924.01.T01:CDS |
| GTGTGTATGTGCATCAATTG+GGG | 0.607210 | 7:+84322089 | MsG0780040924.01.T01:CDS |
| AACCAATGCCTTGACAAACA+AGG | 0.613471 | 7:+84322027 | MsG0780040924.01.T01:CDS |
| AGCCGGTAAAGTCGCTGCTG+AGG | 0.614776 | 7:+84321781 | MsG0780040924.01.T01:CDS |
| TACACAAGTCTCAGAAACAG+TGG | 0.674560 | 7:+84322067 | MsG0780040924.01.T01:CDS |
| TGTGTATGTGCATCAATTGG+GGG | 0.734443 | 7:+84322090 | MsG0780040924.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGGTTTATATTGATATTG+GGG | + | Chr7:84321601-84321620 | MsG0780040924.01.T01:CDS | 25.0% |
| !!! | AGGTTGGTTTATATTGATAT+TGG | + | Chr7:84321599-84321618 | MsG0780040924.01.T01:CDS | 25.0% |
| !!! | GGTTGGTTTATATTGATATT+GGG | + | Chr7:84321600-84321619 | MsG0780040924.01.T01:CDS | 25.0% |
| AACTTAGGCAAATATGAAAC+CGG | - | Chr7:84321557-84321576 | None:intergenic | 30.0% | |
| GTAGAAACATCTACAAACTT+AGG | - | Chr7:84321572-84321591 | None:intergenic | 30.0% | |
| GTTTATATTGATATTGGGGT+AGG | + | Chr7:84321605-84321624 | MsG0780040924.01.T01:CDS | 30.0% | |
| TGAATGCAAATGTTAGTGAT+TGG | + | Chr7:84321636-84321655 | MsG0780040924.01.T01:CDS | 30.0% | |
| TTATCTGATGTATTTGACAG+TGG | + | Chr7:84321944-84321963 | MsG0780040924.01.T01:CDS | 30.0% | |
| TTCTGATCAATAGGATATGA+AGG | - | Chr7:84321668-84321687 | None:intergenic | 30.0% | |
| TTGAAAGCTTTCTGATCAAT+AGG | - | Chr7:84321677-84321696 | None:intergenic | 30.0% | |
| ! | AGTTTGTAGATGTTTCTACT+AGG | + | Chr7:84321573-84321592 | MsG0780040924.01.T01:CDS | 30.0% |
| ! | TTTTACACCATAATCACCAA+AGG | - | Chr7:84322155-84322174 | None:intergenic | 30.0% |
| ATCACGTTTGTTTATCATCC+TGG | + | Chr7:84321755-84321774 | MsG0780040924.01.T01:CDS | 35.0% | |
| GACAAACAAGGAAAATTGCA+TGG | + | Chr7:84322039-84322058 | MsG0780040924.01.T01:CDS | 35.0% | |
| TGATAAACAAACGTGATACC+AGG | - | Chr7:84321752-84321771 | None:intergenic | 35.0% | |
| TTATCACCATCTTCTTTCTC+AGG | - | Chr7:84322007-84322026 | None:intergenic | 35.0% | |
| ! | TAGATGTTTCTACTAGGAAG+AGG | + | Chr7:84321579-84321598 | MsG0780040924.01.T01:CDS | 35.0% |
| ! | TGTTTAGCCTTTGGTGATTA+TGG | + | Chr7:84322145-84322164 | MsG0780040924.01.T01:CDS | 35.0% |
| !! | GCAATTTTCCTTGTTTGTCA+AGG | - | Chr7:84322038-84322057 | None:intergenic | 35.0% |
| AACCAATGCCTTGACAAACA+AGG | + | Chr7:84322027-84322046 | MsG0780040924.01.T01:CDS | 40.0% | |
| AAGGCTAAACACCTAAGGTT+TGG | - | Chr7:84322136-84322155 | None:intergenic | 40.0% | |
| AGATGTCCTGAGAAAGAAGA+TGG | + | Chr7:84321998-84322017 | MsG0780040924.01.T01:CDS | 40.0% | |
| GGTGTGTATGTGCATCAATT+GGG | + | Chr7:84322088-84322107 | MsG0780040924.01.T01:CDS | 40.0% | |
| GTGTGTATGTGCATCAATTG+GGG | + | Chr7:84322089-84322108 | MsG0780040924.01.T01:CDS | 40.0% | |
| GTTGTACTCAAGATGAATGC+AGG | + | Chr7:84321902-84321921 | MsG0780040924.01.T01:CDS | 40.0% | |
| TACACAAGTCTCAGAAACAG+TGG | + | Chr7:84322067-84322086 | MsG0780040924.01.T01:CDS | 40.0% | |
| TGCATACAAGACCAAACCTT+AGG | + | Chr7:84322122-84322141 | MsG0780040924.01.T01:CDS | 40.0% | |
| TGGTGTGTATGTGCATCAAT+TGG | + | Chr7:84322087-84322106 | MsG0780040924.01.T01:CDS | 40.0% | |
| TGTGTATGTGCATCAATTGG+GGG | + | Chr7:84322090-84322109 | MsG0780040924.01.T01:CDS | 40.0% | |
| TTGCACAGTTTCTTTGAACC+AGG | - | Chr7:84321870-84321889 | None:intergenic | 40.0% | |
| ! | AACCTTAGGTGTTTAGCCTT+TGG | + | Chr7:84322136-84322155 | MsG0780040924.01.T01:CDS | 40.0% |
| ! | AAGAGTTTGATTTCCTTGCC+TGG | + | Chr7:84321849-84321868 | MsG0780040924.01.T01:CDS | 40.0% |
| ! | TGTTTCTACTAGGAAGAGGT+TGG | + | Chr7:84321583-84321602 | MsG0780040924.01.T01:CDS | 40.0% |
| ! | TTCCTTGTTTGTCAAGGCAT+TGG | - | Chr7:84322032-84322051 | None:intergenic | 40.0% |
| !! | GTTTATCATCCTGGATTAGC+CGG | + | Chr7:84321764-84321783 | MsG0780040924.01.T01:CDS | 40.0% |
| AACTCTTCCTCCCCAACAAA+AGG | - | Chr7:84321836-84321855 | None:intergenic | 45.0% | |
| CACCAAAGGCTAAACACCTA+AGG | - | Chr7:84322141-84322160 | None:intergenic | 45.0% | |
| CAGTTTCTTTGAACCAGGCA+AGG | - | Chr7:84321865-84321884 | None:intergenic | 45.0% | |
| GGCAAATATGAAACCGGCAT+CGG | - | Chr7:84321551-84321570 | None:intergenic | 45.0% | |
| TTGTCTTACGTGAAGAGACC+TGG | + | Chr7:84321731-84321750 | MsG0780040924.01.T01:CDS | 45.0% | |
| ! | AGAGCTGGATCCTTTTGTTG+GGG | + | Chr7:84321823-84321842 | MsG0780040924.01.T01:CDS | 45.0% |
| ! | GAGAGCTGGATCCTTTTGTT+GGG | + | Chr7:84321822-84321841 | MsG0780040924.01.T01:CDS | 45.0% |
| ! | GAGGCTGATAATGTCGGAGA+CGG | + | Chr7:84321800-84321819 | MsG0780040924.01.T01:CDS | 50.0% |
| ! | GCTGCTGAGGCTGATAATGT+CGG | + | Chr7:84321794-84321813 | MsG0780040924.01.T01:CDS | 50.0% |
| ! | GGAGAGCTGGATCCTTTTGT+TGG | + | Chr7:84321821-84321840 | MsG0780040924.01.T01:CDS | 50.0% |
| AGCCTCAGCAGCGACTTTAC+CGG | - | Chr7:84321786-84321805 | None:intergenic | 55.0% | |
| TAATGTCGGAGACGGAGAGC+TGG | + | Chr7:84321808-84321827 | MsG0780040924.01.T01:CDS | 55.0% | |
| ! | GCGACTTTACCGGCTAATCC+AGG | - | Chr7:84321776-84321795 | None:intergenic | 55.0% |
| ! | GCTGGATCCTTTTGTTGGGG+AGG | + | Chr7:84321826-84321845 | MsG0780040924.01.T01:CDS | 55.0% |
| AGCCGGTAAAGTCGCTGCTG+AGG | + | Chr7:84321781-84321800 | MsG0780040924.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 84321545 | 84322180 | 84321545 | ID=MsG0780040924.01;Name=MsG0780040924.01 |
| Chr7 | mRNA | 84321545 | 84322180 | 84321545 | ID=MsG0780040924.01.T01;Parent=MsG0780040924.01;Name=MsG0780040924.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|211 |
| Chr7 | exon | 84321545 | 84322180 | 84321545 | ID=MsG0780040924.01.T01:exon:40708;Parent=MsG0780040924.01.T01 |
| Chr7 | CDS | 84321545 | 84322180 | 84321545 | ID=MsG0780040924.01.T01:cds;Parent=MsG0780040924.01.T01 |
| Gene Sequence |
| Protein sequence |
