Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041056.01.T01 | KEH24088.1 | 92.5 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 1.28E-59 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041056.01.T01 | Q8S9J0 | 63.717 | 113 | 34 | 1 | 8 | 120 | 12 | 117 | 1.94E-29 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041056.01.T01 | A0A072U4M3 | 92.500 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 6.11e-60 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024269.01 | MsG0780041056.01 | 0.824253 | 8.922540e-54 | 7.861057e-51 |
| MsG0380016528.01 | MsG0780041056.01 | 0.850445 | 1.690898e-60 | 3.312993e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041056.01.T01 | MTR_7g103300 | 92.500 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 1.55e-63 | 197 |
| MsG0780041056.01.T01 | MTR_7g103300 | 92.500 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 4.27e-63 | 197 |
| MsG0780041056.01.T01 | MTR_7g103300 | 92.500 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 1.15e-62 | 197 |
| MsG0780041056.01.T01 | MTR_7g103300 | 92.500 | 120 | 9 | 0 | 1 | 120 | 1 | 120 | 2.35e-62 | 197 |
| MsG0780041056.01.T01 | MTR_7g103300 | 98.462 | 65 | 1 | 0 | 56 | 120 | 8 | 72 | 4.63e-36 | 127 |
| MsG0780041056.01.T01 | MTR_1g061710 | 63.492 | 126 | 35 | 2 | 1 | 120 | 1 | 121 | 8.43e-35 | 125 |
| MsG0780041056.01.T01 | MTR_8g077830 | 62.025 | 79 | 30 | 0 | 42 | 120 | 54 | 132 | 1.04e-19 | 83.6 |
| MsG0780041056.01.T01 | MTR_5g019950 | 63.514 | 74 | 27 | 0 | 47 | 120 | 66 | 139 | 2.48e-19 | 82.4 |
| MsG0780041056.01.T01 | MTR_3g109950 | 52.427 | 103 | 46 | 2 | 21 | 120 | 34 | 136 | 2.62e-19 | 82.4 |
| MsG0780041056.01.T01 | MTR_3g109950 | 52.427 | 103 | 46 | 2 | 21 | 120 | 34 | 136 | 2.64e-19 | 82.4 |
| MsG0780041056.01.T01 | MTR_3g109950 | 52.427 | 103 | 46 | 2 | 21 | 120 | 34 | 136 | 3.11e-19 | 82.0 |
| MsG0780041056.01.T01 | MTR_1g026910 | 46.809 | 94 | 47 | 1 | 27 | 120 | 26 | 116 | 3.66e-17 | 76.3 |
| MsG0780041056.01.T01 | MTR_1g026910 | 46.809 | 94 | 47 | 1 | 27 | 120 | 26 | 116 | 3.87e-17 | 76.3 |
| MsG0780041056.01.T01 | MTR_8g088080 | 52.083 | 96 | 44 | 1 | 25 | 120 | 42 | 135 | 2.33e-16 | 73.9 |
| MsG0780041056.01.T01 | MTR_8g088070 | 51.515 | 99 | 44 | 3 | 25 | 120 | 32 | 129 | 1.79e-13 | 65.9 |
| MsG0780041056.01.T01 | MTR_7g103300 | 100.000 | 30 | 0 | 0 | 91 | 120 | 1 | 30 | 1.47e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041056.01.T01 | AT2G36630 | 63.717 | 113 | 34 | 1 | 8 | 120 | 12 | 117 | 1.98e-30 | 113 |
| MsG0780041056.01.T01 | AT2G25737 | 69.118 | 68 | 21 | 0 | 53 | 120 | 70 | 137 | 5.18e-20 | 84.0 |
| MsG0780041056.01.T01 | AT2G25737 | 69.118 | 68 | 21 | 0 | 53 | 120 | 70 | 137 | 8.52e-20 | 84.0 |
Find 33 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGTCAATTGCGAATTTC+TGG | 0.142310 | 7:-85938149 | None:intergenic |
| CCTATGCTTACTCTTATTAT+TGG | 0.163814 | 7:+85938529 | MsG0780041056.01.T01:CDS |
| GCCTCAATTATTGGCTTTCT+AGG | 0.224694 | 7:+85938457 | MsG0780041056.01.T01:CDS |
| CTTGCTTTGGCCTCAATTAT+TGG | 0.227645 | 7:+85938448 | MsG0780041056.01.T01:CDS |
| GGCTTTCTAGGATCTGCTTT+TGG | 0.237402 | 7:+85938469 | MsG0780041056.01.T01:CDS |
| GTTATTTATAGGTATGATAA+TGG | 0.318227 | 7:+85938679 | MsG0780041056.01.T01:intron |
| GCCTAGTTGGAGACTTGCTT+TGG | 0.339589 | 7:+85938435 | MsG0780041056.01.T01:CDS |
| GAGGTAGAGGATGAATGTTT+TGG | 0.388535 | 7:-85938185 | None:intergenic |
| GAGAATCGCAACGGAGAAAC+CGG | 0.412752 | 7:-85938212 | None:intergenic |
| TTCATCCTCTACCTCGTATC+CGG | 0.430162 | 7:+85938193 | MsG0780041056.01.T01:CDS |
| TGCAGGAATTGGAGCCTAGT+TGG | 0.435667 | 7:+85938422 | MsG0780041056.01.T01:intron |
| TTATTTATAGGTATGATAAT+GGG | 0.441362 | 7:+85938680 | MsG0780041056.01.T01:intron |
| ACCGTTGGTGGTGTTGGTGG+TGG | 0.448909 | 7:+85938493 | MsG0780041056.01.T01:CDS |
| GGGAGGGAGTAACGTTGTTA+TGG | 0.452115 | 7:-85938105 | None:intergenic |
| GTTGGTGGTGTTGGTGGTGG+TGG | 0.454327 | 7:+85938496 | MsG0780041056.01.T01:CDS |
| TCTCCATCTCTTCAATTCCA+TGG | 0.464521 | 7:+85938280 | MsG0780041056.01.T01:CDS |
| TTTGGAACCGTTGGTGGTGT+TGG | 0.485288 | 7:+85938487 | MsG0780041056.01.T01:CDS |
| GGAAAGAGCAGCAGCAGACT+TGG | 0.486427 | 7:-85938560 | None:intergenic |
| GGAACCGTTGGTGGTGTTGG+TGG | 0.502109 | 7:+85938490 | MsG0780041056.01.T01:CDS |
| ACCACCACCAACACCACCAA+CGG | 0.547510 | 7:-85938494 | None:intergenic |
| AATCCAAAACCGATAAAACT+TGG | 0.553743 | 7:+85938311 | MsG0780041056.01.T01:CDS |
| GAGCCATGGAATTGAAGAGA+TGG | 0.569660 | 7:-85938283 | None:intergenic |
| TCCTAGAAAGCCAATAATTG+AGG | 0.572327 | 7:-85938458 | None:intergenic |
| CAACAAAAGACACAGAGGGA+GGG | 0.589945 | 7:-85938121 | None:intergenic |
| ACAACAAAAGACACAGAGGG+AGG | 0.600323 | 7:-85938122 | None:intergenic |
| AGAAACCGGATACGAGGTAG+AGG | 0.604035 | 7:-85938198 | None:intergenic |
| GCCAAAGCAAGTCTCCAACT+AGG | 0.614402 | 7:-85938436 | None:intergenic |
| CAACGGAGAAACCGGATACG+AGG | 0.616746 | 7:-85938204 | None:intergenic |
| TTTGGTGGAAAAGGACATAG+TGG | 0.622803 | 7:-85938167 | None:intergenic |
| CCAATAATAAGAGTAAGCAT+AGG | 0.636816 | 7:-85938529 | None:intergenic |
| AACAACAACAAAAGACACAG+AGG | 0.648660 | 7:-85938126 | None:intergenic |
| ACAACAACAAAAGACACAGA+GGG | 0.648796 | 7:-85938125 | None:intergenic |
| AAGAGCGGAGAGAATCGCAA+CGG | 0.698430 | 7:-85938221 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTATAGGTATGATAAT+GGG | + | Chr7:85938680-85938699 | MsG0780041056.01.T01:intron | 15.0% |
| !! | AAAACGGAATTAATTACATT+TGG | - | Chr7:85938584-85938603 | None:intergenic | 20.0% |
| !! | AAATGAAACCTTATGTAAAA+CGG | - | Chr7:85938600-85938619 | None:intergenic | 20.0% |
| !! | GTTATTTATAGGTATGATAA+TGG | + | Chr7:85938679-85938698 | MsG0780041056.01.T01:intron | 20.0% |
| !!! | AATTAATTCCGTTTTACATA+AGG | + | Chr7:85938589-85938608 | MsG0780041056.01.T01:intron | 20.0% |
| !!! | CAAAGAGTTAATGTTTTTAA+CGG | - | Chr7:85938397-85938416 | None:intergenic | 20.0% |
| !!! | TTGTTGACTATGTTATTTAT+AGG | + | Chr7:85938668-85938687 | MsG0780041056.01.T01:intron | 20.0% |
| AATCCAAAACCGATAAAACT+TGG | + | Chr7:85938311-85938330 | MsG0780041056.01.T01:CDS | 30.0% | |
| CCAATAATAAGAGTAAGCAT+AGG | - | Chr7:85938532-85938551 | None:intergenic | 30.0% | |
| CCTATGCTTACTCTTATTAT+TGG | + | Chr7:85938529-85938548 | MsG0780041056.01.T01:CDS | 30.0% | |
| ! | AGATGAATTTTCGTGGTAAT+TGG | - | Chr7:85938263-85938282 | None:intergenic | 30.0% |
| ! | ATTAACTCTTTGTTTGTTGC+AGG | + | Chr7:85938405-85938424 | MsG0780041056.01.T01:intron | 30.0% |
| AACAACAACAAAAGACACAG+AGG | - | Chr7:85938129-85938148 | None:intergenic | 35.0% | |
| AACGTGTTGTGAGTAACATA+CGG | - | Chr7:85938338-85938357 | None:intergenic | 35.0% | |
| ACAACAACAAAAGACACAGA+GGG | - | Chr7:85938128-85938147 | None:intergenic | 35.0% | |
| CAATGCTTGATTAGAAACAG+AGG | + | Chr7:85938620-85938639 | MsG0780041056.01.T01:intron | 35.0% | |
| TCCTAGAAAGCCAATAATTG+AGG | - | Chr7:85938461-85938480 | None:intergenic | 35.0% | |
| ! | ATTTTGTGTGAGGTAAAGAG+CGG | - | Chr7:85938239-85938258 | None:intergenic | 35.0% |
| ! | GGTAATTGGTATTTTGTGTG+AGG | - | Chr7:85938249-85938268 | None:intergenic | 35.0% |
| ! | TCTTTGTTTGTTGCAGGAAT+TGG | + | Chr7:85938411-85938430 | MsG0780041056.01.T01:intron | 35.0% |
| !!! | GATGAATGTTTTGGTGGAAA+AGG | - | Chr7:85938179-85938198 | None:intergenic | 35.0% |
| ACGTGTTGTGAGTAACATAC+GGG | - | Chr7:85938337-85938356 | None:intergenic | 40.0% | |
| AGTGGTCAATTGCGAATTTC+TGG | - | Chr7:85938152-85938171 | None:intergenic | 40.0% | |
| CTTGCTTTGGCCTCAATTAT+TGG | + | Chr7:85938448-85938467 | MsG0780041056.01.T01:CDS | 40.0% | |
| TCTCCATCTCTTCAATTCCA+TGG | + | Chr7:85938280-85938299 | MsG0780041056.01.T01:CDS | 40.0% | |
| TTTGGTGGAAAAGGACATAG+TGG | - | Chr7:85938170-85938189 | None:intergenic | 40.0% | |
| ! | GAGATGGAGATGAATTTTCG+TGG | - | Chr7:85938270-85938289 | None:intergenic | 40.0% |
| ! | GAGGTAGAGGATGAATGTTT+TGG | - | Chr7:85938188-85938207 | None:intergenic | 40.0% |
| ! | TTTTGGATTCGTAAGAGCCA+TGG | - | Chr7:85938300-85938319 | None:intergenic | 40.0% |
| !! | ACATACGGGCCAAGTTTTAT+CGG | - | Chr7:85938323-85938342 | None:intergenic | 40.0% |
| !! | GCCTCAATTATTGGCTTTCT+AGG | + | Chr7:85938457-85938476 | MsG0780041056.01.T01:CDS | 40.0% |
| !! | GTAGAGGATGAATGTTTTGG+TGG | - | Chr7:85938185-85938204 | None:intergenic | 40.0% |
| ACAACAAAAGACACAGAGGG+AGG | - | Chr7:85938125-85938144 | None:intergenic | 45.0% | |
| CAACAAAAGACACAGAGGGA+GGG | - | Chr7:85938124-85938143 | None:intergenic | 45.0% | |
| GAGCCATGGAATTGAAGAGA+TGG | - | Chr7:85938286-85938305 | None:intergenic | 45.0% | |
| GTTACTCACAACACGTTCTC+AGG | + | Chr7:85938341-85938360 | MsG0780041056.01.T01:intron | 45.0% | |
| TTCATCCTCTACCTCGTATC+CGG | + | Chr7:85938193-85938212 | MsG0780041056.01.T01:CDS | 45.0% | |
| !!! | GGCTTTCTAGGATCTGCTTT+TGG | + | Chr7:85938469-85938488 | MsG0780041056.01.T01:CDS | 45.0% |
| !!! | GGGCCAAGTTTTATCGGTTT+TGG | - | Chr7:85938317-85938336 | None:intergenic | 45.0% |
| AAGAGCGGAGAGAATCGCAA+CGG | - | Chr7:85938224-85938243 | None:intergenic | 50.0% | |
| AGAAACCGGATACGAGGTAG+AGG | - | Chr7:85938201-85938220 | None:intergenic | 50.0% | |
| GAGAATCGCAACGGAGAAAC+CGG | - | Chr7:85938215-85938234 | None:intergenic | 50.0% | |
| GCCAAAGCAAGTCTCCAACT+AGG | - | Chr7:85938439-85938458 | None:intergenic | 50.0% | |
| GGGAGGGAGTAACGTTGTTA+TGG | - | Chr7:85938108-85938127 | None:intergenic | 50.0% | |
| TGCAGGAATTGGAGCCTAGT+TGG | + | Chr7:85938422-85938441 | MsG0780041056.01.T01:intron | 50.0% | |
| ! | GCCTAGTTGGAGACTTGCTT+TGG | + | Chr7:85938435-85938454 | MsG0780041056.01.T01:CDS | 50.0% |
| !! | TTTGGAACCGTTGGTGGTGT+TGG | + | Chr7:85938487-85938506 | MsG0780041056.01.T01:CDS | 50.0% |
| !!! | GGATCTGCTTTTGGAACCGT+TGG | + | Chr7:85938478-85938497 | MsG0780041056.01.T01:CDS | 50.0% |
| !!! | TCTGCTTTTGGAACCGTTGG+TGG | + | Chr7:85938481-85938500 | MsG0780041056.01.T01:CDS | 50.0% |
| ACCACCACCAACACCACCAA+CGG | - | Chr7:85938497-85938516 | None:intergenic | 55.0% | |
| CAACGGAGAAACCGGATACG+AGG | - | Chr7:85938207-85938226 | None:intergenic | 55.0% | |
| GGAAAGAGCAGCAGCAGACT+TGG | - | Chr7:85938563-85938582 | None:intergenic | 55.0% | |
| !! | ACCGTTGGTGGTGTTGGTGG+TGG | + | Chr7:85938493-85938512 | MsG0780041056.01.T01:CDS | 60.0% |
| !! | GGAACCGTTGGTGGTGTTGG+TGG | + | Chr7:85938490-85938509 | MsG0780041056.01.T01:CDS | 60.0% |
| !! | GTTGGTGGTGTTGGTGGTGG+TGG | + | Chr7:85938496-85938515 | MsG0780041056.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 85938087 | 85938733 | 85938087 | ID=MsG0780041056.01;Name=MsG0780041056.01 |
| Chr7 | mRNA | 85938087 | 85938733 | 85938087 | ID=MsG0780041056.01.T01;Parent=MsG0780041056.01;Name=MsG0780041056.01.T01;_AED=0.42;_eAED=0.42;_QI=85|1|1|1|0.33|0.53|13|0|122 |
| Chr7 | exon | 85938087 | 85938336 | 85938087 | ID=MsG0780041056.01.T01:exon:17932;Parent=MsG0780041056.01.T01 |
| Chr7 | exon | 85938427 | 85938586 | 85938427 | ID=MsG0780041056.01.T01:exon:17933;Parent=MsG0780041056.01.T01 |
| Chr7 | exon | 85938690 | 85938733 | 85938690 | ID=MsG0780041056.01.T01:exon:17934;Parent=MsG0780041056.01.T01 |
| Chr7 | five_prime_UTR | 85938087 | 85938171 | 85938087 | ID=MsG0780041056.01.T01:five_prime_utr;Parent=MsG0780041056.01.T01 |
| Chr7 | CDS | 85938172 | 85938336 | 85938172 | ID=MsG0780041056.01.T01:cds;Parent=MsG0780041056.01.T01 |
| Chr7 | CDS | 85938427 | 85938586 | 85938427 | ID=MsG0780041056.01.T01:cds;Parent=MsG0780041056.01.T01 |
| Chr7 | CDS | 85938690 | 85938733 | 85938690 | ID=MsG0780041056.01.T01:cds;Parent=MsG0780041056.01.T01 |
| Gene Sequence |
| Protein sequence |
