AlfalfaGEDB | MsG0780041217.01

M. sativa cultivar ZhongmuNo.1 / MsG0780041217.01

KEGG: K15849
GO: GO:0003824 GO:0009058 GO:0030170
PFAM: PF00155

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041217.01.T01	XP_013450261.1	88.063	511	23	2	1	511	1	473	0	903

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041217.01.T01	E9L7A5	71.66	494	101	4	14	503	13	471	0	705

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041217.01.T01	A0A072U3J0	88.063	511	23	2	1	511	1	473	0.0	903

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180005113.01	MsG0780041217.01	PPI
MsG0180005153.01	MsG0780041217.01	PPI
MsG0380015567.01	MsG0780041217.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041217.01.T01	MTR_7g106830	88.063	511	23	2	1	511	1	473	0.0	903
MsG0780041217.01.T01	MTR_8g091280	79.337	513	66	4	1	511	1	475	0.0	804
MsG0780041217.01.T01	MTR_1807s0010	35.238	420	237	3	80	498	7	392	7.07e-76	244
MsG0780041217.01.T01	MTR_3g072670	28.266	421	247	11	88	502	72	443	1.72e-45	165
MsG0780041217.01.T01	MTR_3g072680	27.861	402	237	10	107	502	46	400	2.45e-43	159
MsG0780041217.01.T01	MTR_4g011820	27.792	385	218	10	135	511	62	394	1.10e-37	143
MsG0780041217.01.T01	MTR_1g087760	29.952	414	223	12	107	502	30	394	4.58e-36	139
MsG0780041217.01.T01	MTR_3g072690	35.577	208	128	5	107	312	11	214	6.35e-33	128
MsG0780041217.01.T01	MTR_8g061360	21.760	409	262	12	106	499	42	407	3.13e-16	81.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041217.01.T01	AT2G22250	68.352	455	110	3	53	505	47	469	0.0	625
MsG0780041217.01.T01	AT2G22250	68.352	455	110	3	53	505	47	469	0.0	625
MsG0780041217.01.T01	AT2G22250	70.439	433	97	1	73	505	21	422	0.0	625
MsG0780041217.01.T01	AT1G77670	27.540	443	263	12	66	502	46	436	4.97e-49	175
MsG0780041217.01.T01	AT1G80360	26.823	384	218	11	137	511	64	393	9.52e-33	129
MsG0780041217.01.T01	AT1G80360	26.823	384	218	11	137	511	64	393	9.52e-33	129
MsG0780041217.01.T01	AT1G80360	26.823	384	218	11	137	511	64	393	9.52e-33	129
MsG0780041217.01.T01	AT1G80360	26.823	384	218	11	137	511	64	393	9.52e-33	129
MsG0780041217.01.T01	AT5G36160	20.091	438	285	11	84	500	17	410	4.29e-17	84.0
MsG0780041217.01.T01	AT5G53970	21.376	407	276	10	101	499	30	400	1.84e-15	79.0

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Find 126 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTGCAGTTGCTGCATC+AGG	0.433618	7:-88356118	MsG0780041217.01.T01:CDS
AAATTATCAGATATTGACGT+TGG	0.562680	7:+88356983	None:intergenic
AACCTTAGAAAATCCATTCA+CGG	0.462409	7:+88356620	None:intergenic
AACGCCTGCTTGAACAAGAG+CGG	0.668953	7:+88358454	None:intergenic
AAGGATTCACACCTGTGGTT+CGG	0.490759	7:+88355988	None:intergenic
AATAAACTTGGATGATCCTA+AGG	0.494545	7:-88356494	MsG0780041217.01.T01:CDS
ACAAGGTACACCCGTAATGC+GGG	0.533223	7:-88357406	MsG0780041217.01.T01:CDS
ACAAGTGCAGCTGAGGTAAG+AGG	0.413408	7:+88355549	None:intergenic
ACACTAGACAAGTGCAGCTG+AGG	0.616702	7:+88355542	None:intergenic
ACAGATCCTGTCGGGTTAGA+CGG	0.476864	7:+88356893	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCTTAGAAAATCCATTCA+CGG	+	Chr7:88357671-88357690	None:intergenic	30.0%
AATAAACTTGGATGATCCTA+AGG	-	Chr7:88357794-88357813	MsG0780041217.01.T01:intron	30.0%
ATAACTTTACCAAAAGCCTT+GGG	+	Chr7:88357469-88357488	None:intergenic	30.0%
ATTGAAAAAACCTACGAAGT+AGG	+	Chr7:88356520-88356539	None:intergenic	30.0%
CATAAGCTGAAAACAAATCA+AGG	+	Chr7:88356636-88356655	None:intergenic	30.0%
GAGTATGCAAATAAACTAGA+AGG	+	Chr7:88358322-88358341	None:intergenic	30.0%
GGAATAATGACCTGAAATAT+TGG	+	Chr7:88357221-88357240	None:intergenic	30.0%
GTAAAGTTATACTTCTCTGA+TGG	-	Chr7:88357479-88357498	MsG0780041217.01.T01:intron	30.0%
GTTGTTATATAACATGTTGG+TGG	+	Chr7:88355552-88355571	None:intergenic	30.0%
TATAACTTTACCAAAAGCCT+TGG	+	Chr7:88357470-88357489	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	88355545	88358765	88355545	ID=MsG0780041217.01;Name=MsG0780041217.01
Chr7	mRNA	88355545	88358765	88355545	ID=MsG0780041217.01.T01;Parent=MsG0780041217.01;Name=MsG0780041217.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|9\|0\|511
Chr7	exon	88358394	88358765	88358394	ID=MsG0780041217.01.T01:exon:52346;Parent=MsG0780041217.01.T01
Chr7	exon	88357365	88357458	88357365	ID=MsG0780041217.01.T01:exon:52345;Parent=MsG0780041217.01.T01
Chr7	exon	88357172	88357275	88357172	ID=MsG0780041217.01.T01:exon:52344;Parent=MsG0780041217.01.T01
Chr7	exon	88356832	88357080	88356832	ID=MsG0780041217.01.T01:exon:52343;Parent=MsG0780041217.01.T01
Chr7	exon	88356594	88356736	88356594	ID=MsG0780041217.01.T01:exon:52342;Parent=MsG0780041217.01.T01
Chr7	exon	88356387	88356534	88356387	ID=MsG0780041217.01.T01:exon:52341;Parent=MsG0780041217.01.T01
Chr7	exon	88356000	88356170	88356000	ID=MsG0780041217.01.T01:exon:52340;Parent=MsG0780041217.01.T01
Chr7	exon	88355795	88355908	88355795	ID=MsG0780041217.01.T01:exon:52339;Parent=MsG0780041217.01.T01
Chr7	exon	88355545	88355685	88355545	ID=MsG0780041217.01.T01:exon:52338;Parent=MsG0780041217.01.T01
Chr7	CDS	88358394	88358765	88358394	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88357365	88357458	88357365	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88357172	88357275	88357172	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88356832	88357080	88356832	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88356594	88356736	88356594	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88356387	88356534	88356387	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88356000	88356170	88356000	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88355795	88355908	88355795	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01
Chr7	CDS	88355545	88355685	88355545	ID=MsG0780041217.01.T01:cds;Parent=MsG0780041217.01.T01

Gene Sequence

>MsG0780041217.01.T01

ATGGCCACCAACATGTTATATAACAACACCACATGTGGAATCTCTTTCCCTCACCAATCTCTCCCCGCAAAGTCTGCCTCTCTCCACGTCTCTTTCGGCTCAAACCTTTCCACGTTCCCCCACAGTACTGTAAAATATGTTGTTGGAGTGGAGCAGAACAGAAGAGGAAGAATAATTGTGAAGTCAAAAACCGTTTCTGATAATGATGATTTAGAAGTTGATATTTCATTGAGTCCACGAGTCAATGCTGTGAAACCTTCAAGGACTGTGTCTATAACTGATCAAGCCACCGCTCTTGTTCAAGCAGGCGTTCCTGTTATTCTCTTGGCGGCTGGTGAGCCTGATTTTGATACACCTGCTGTCATAGCTCAGGCTGGGATTAATGCAATTCATGAAGGACACACAAGGTACACCCGTAATGCGGGAACTTTAGAATTGCGCCAAGCAATTTGTCATAAACTAAAAGAGGAGAATGGAATTGCTTATAATACTGATCAGATTATTGTTAGTAATGGAGCCAAGCAGAGTATTGCTCAGGCAATGCTTGCAGTTTGCTCTCCAGGGGATGAGGTCATTATTCCAGCTCCATTCTATGTTAGTTACCCAGAAATGGCAAGGATGGCTAATGCAACACCCGTTATCCTTCCAACGTCAATATCTGATAATTTCCTTTTAGATCCCAAACTTCTTGAATCCATAATAAGTGAAAGGTCAAGACTGCTTATTCTTTGTTCGCCGTCTAACCCGACAGGATCTGTCTATCCTAAGAAATTACTTGAAGAGATAGCCCATATTGTAGCAAAGCACCCAAGGCTTTTGGTTATCTCTGATGAAATTTATGAACACATAATTTATGCACCAGCAACACACACTAGCTTTGCATCTTTACCTGGAATGTGGGATAAAACGCTAACCGTGAATGGATTTTCTAAGGTTAGTCCATATGTCATGTTCTCTTTCTGTATGGTTATAAATTATGAAATAAACTTGGATGATCCTAAGGCTTCTAATTTTTTTTTACTGCAGCCCTTTGCAATGACTGGTTGGCGGCTTGGTTATATTGCAGGCCCGAAACATTTTATTTCAGCATGTGGAAAGATTCAAAGTCAGTTTACTTCGGGGGCCAGTAGTATATCTCAGAAAGCTGCAGTTGCTGCATCAGGACTAGGCTATGCTGGTGGAGAGGTTGTTTCTACCATGGTGAAAGCATTTAGAGAACGAAGGAATTACTTGGTTAAAAGTTTTAGCGAAATCGATGGTGTTGAAATATCCGAACCACAGGGAGCATTTTATTTATTCATTGATTTCAGCTCCTATTATGGAAGAGAAGCTGAAGGTTTTGGCAAAATTGAGGATTCTGAGTCTCTTTGTCAATATCTGTTGCATAAAGGCCAGGTCGCACTTGTGCCAGGGAGTGCATCTGGAGACGATTCTTGCATTCGCATCTCGTATGCAGCATCTCTTCCTACCCTACAGGCAGCTGTAGAGAGAATTAAGAAAGCACTTATCCCTCTTACCTCAGCTGCACTTGTCTAG

Protein sequence

>MsG0780041217.01.T01

MATNMLYNNTTCGISFPHQSLPAKSASLHVSFGSNLSTFPHSTVKYVVGVEQNRRGRIIVKSKTVSDNDDLEVDISLSPRVNAVKPSRTVSITDQATALVQAGVPVILLAAGEPDFDTPAVIAQAGINAIHEGHTRYTRNAGTLELRQAICHKLKEENGIAYNTDQIIVSNGAKQSIAQAMLAVCSPGDEVIIPAPFYVSYPEMARMANATPVILPTSISDNFLLDPKLLESIISERSRLLILCSPSNPTGSVYPKKLLEEIAHIVAKHPRLLVISDEIYEHIIYAPATHTSFASLPGMWDKTLTVNGFSKVSPYVMFSFCMVINYEINLDDPKASNFFLLQPFAMTGWRLGYIAGPKHFISACGKIQSQFTSGASSISQKAAVAASGLGYAGGEVVSTMVKAFRERRNYLVKSFSEIDGVEISEPQGAFYLFIDFSSYYGREAEGFGKIEDSESLCQYLLHKGQVALVPGSASGDDSCIRISYAASLPTLQAAVERIKKALIPLTSAALV*