AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041805.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041805.01.T01 MTR_7g118230 89.286 28 3 0 30 57 119 146 2.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGACTCTTTGGTAAATT+TGG 0.141330 7:+95360620 MsG0780041805.01.T01:CDS
GCACAGAGAATATTGCATTT+TGG 0.195973 7:+95360920 MsG0780041805.01.T01:intron
CCAAAGAGTCTCACGTCTTC+AGG 0.277326 7:-95360611 None:intergenic
CCTGAAGACGTGAGACTCTT+TGG 0.364197 7:+95360611 MsG0780041805.01.T01:CDS
ATGCTGTGCTGTAGGTTTGC+CGG 0.436603 7:+95360466 MsG0780041805.01.T01:intron
TTCCCAACATACACCTTATG+AGG 0.456223 7:-95360569 None:intergenic
ATCTCAACAGAACATTTAAC+CGG 0.456281 7:-95360485 None:intergenic
CAATCGACGGATGTTGGTTG+TGG 0.482273 7:+95359385 MsG0780041805.01.T01:CDS
AGGAAGGTTCTGCATCATAT+TGG 0.483447 7:-95360983 None:intergenic
GTCCTCATAAGGTGTATGTT+GGG 0.485859 7:+95360567 MsG0780041805.01.T01:CDS
CTTTGGTAAATTTGGAAATG+TGG 0.489376 7:+95360628 MsG0780041805.01.T01:CDS
ATGGAGGAAGCTCTCTGCTC+TGG 0.492067 7:+95360952 MsG0780041805.01.T01:CDS
ATACTATGAAGGTCCTCATA+AGG 0.498833 7:+95360556 MsG0780041805.01.T01:CDS
CCGACACAATCGACGGATGT+TGG 0.515512 7:+95359379 MsG0780041805.01.T01:CDS
CGAGAAAATTCAGCTGGAAG+TGG 0.516205 7:+95361009 MsG0780041805.01.T01:CDS
GGTCCTCATAAGGTGTATGT+TGG 0.523656 7:+95360566 MsG0780041805.01.T01:CDS
GTATATAGTTCTTCTTCTCA+CGG 0.533646 7:-95360537 None:intergenic
CCTGAACGAGAAAATTCAGC+TGG 0.535487 7:+95361003 MsG0780041805.01.T01:CDS
AGAAGAACTATATACTATGA+AGG 0.536527 7:+95360545 MsG0780041805.01.T01:CDS
TATATAGTTCTTCTTCTCAC+GGG 0.541659 7:-95360536 None:intergenic
TTTGATGGAACAATTACAGT+AGG 0.550318 7:-95361038 None:intergenic
AATCGACGGATGTTGGTTGT+GGG 0.565123 7:+95359386 MsG0780041805.01.T01:CDS
GAAAATTCAGCTGGAAGTGG+AGG 0.583137 7:+95361012 MsG0780041805.01.T01:CDS
ATAGTTCTTCTTCTCACGGG+AGG 0.629773 7:-95360533 None:intergenic
CCAACATCCGTCGATTGTGT+CGG 0.664202 7:-95359379 None:intergenic
ATGAACGCCGACACAATCGA+CGG 0.683003 7:+95359372 MsG0780041805.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTATTTAAATTAGTTA+GGG - Chr7:95359878-95359897 None:intergenic 10.0%
!! TTTGTATTTAAATTAGTTAG+GGG - Chr7:95359877-95359896 None:intergenic 15.0%
!!! AAATTATAGTTTTCATAGTT+AGG + Chr7:95360011-95360030 MsG0780041805.01.T01:intron 15.0%
!!! AACTATGAAAACTATAATTT+TGG - Chr7:95360011-95360030 None:intergenic 15.0%
!!! AATTATAGTTTTCATAGTTA+GGG + Chr7:95360012-95360031 MsG0780041805.01.T01:intron 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG - Chr7:95359879-95359898 None:intergenic 15.0%
!! AGAGAATTTAAAGATTATGT+GGG + Chr7:95359791-95359810 MsG0780041805.01.T01:intron 20.0%
!! ATTTGAAAAAAGCTGTAATT+AGG - Chr7:95360898-95360917 None:intergenic 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG - Chr7:95359876-95359895 None:intergenic 20.0%
!!! ATTATAGTTTTCATAGTTAG+GGG + Chr7:95360013-95360032 MsG0780041805.01.T01:intron 20.0%
!!! CAAGTTTTTTTAAAGTTAAG+GGG + Chr7:95360045-95360064 MsG0780041805.01.T01:intron 20.0%
!!! CTTTTGGCTTAATTAAATTT+AGG - Chr7:95360088-95360107 None:intergenic 20.0%
!!! GAAATCTTTCACATTTTATA+TGG + Chr7:95359755-95359774 MsG0780041805.01.T01:intron 20.0%
!!! GCAAGTTTTTTTAAAGTTAA+GGG + Chr7:95360044-95360063 MsG0780041805.01.T01:intron 20.0%
!!! TGCAAGTTTTTTTAAAGTTA+AGG + Chr7:95360043-95360062 MsG0780041805.01.T01:intron 20.0%
!!! TTTAATGGATGAGAAAATTA+TGG + Chr7:95359731-95359750 MsG0780041805.01.T01:intron 20.0%
! AACAGTAGAAAAAGTGAAAA+AGG - Chr7:95360788-95360807 None:intergenic 25.0%
! AGAAGAACTATATACTATGA+AGG + Chr7:95360545-95360564 MsG0780041805.01.T01:CDS 25.0%
! GAGAGAATTTAAAGATTATG+TGG + Chr7:95359790-95359809 MsG0780041805.01.T01:intron 25.0%
! TGTGACAAAAAAGAATTCAA+TGG - Chr7:95359506-95359525 None:intergenic 25.0%
! TTACTTGTGATGTAATGTAA+TGG + Chr7:95360434-95360453 MsG0780041805.01.T01:intron 25.0%
! TTTGAGAGAGATAAATACAA+AGG - Chr7:95360710-95360729 None:intergenic 25.0%
!! ATTAAGCCAAAAGTGTAATT+TGG + Chr7:95360095-95360114 MsG0780041805.01.T01:intron 25.0%
!! ATTTTATACGGTTTCATTGA+GGG - Chr7:95360756-95360775 None:intergenic 25.0%
!! CAAAAGTGTAATTTGGATAA+TGG + Chr7:95360102-95360121 MsG0780041805.01.T01:intron 25.0%
!! CATTATCCAAATTACACTTT+TGG - Chr7:95360104-95360123 None:intergenic 25.0%
!! TATTTTATACGGTTTCATTG+AGG - Chr7:95360757-95360776 None:intergenic 25.0%
!! TTATAGTTTTCATAGTTAGG+GGG + Chr7:95360014-95360033 MsG0780041805.01.T01:intron 25.0%
!!! AGATTATGTGGGTTTTTTTT+AGG + Chr7:95359802-95359821 MsG0780041805.01.T01:intron 25.0%
!!! CATTTTTGGCAGTTTTTTTT+TGG - Chr7:95359946-95359965 None:intergenic 25.0%
AAAGTAAAGGAGGACTTTAA+TGG + Chr7:95359716-95359735 MsG0780041805.01.T01:intron 30.0%
AGTAGAAAAAGTGAAAAAGG+TGG - Chr7:95360785-95360804 None:intergenic 30.0%
ATCTCAACAGAACATTTAAC+CGG - Chr7:95360488-95360507 None:intergenic 30.0%
CTTTGGTAAATTTGGAAATG+TGG + Chr7:95360628-95360647 MsG0780041805.01.T01:CDS 30.0%
GTATATAGTTCTTCTTCTCA+CGG - Chr7:95360540-95360559 None:intergenic 30.0%
TACAAAGGTTAAGATAGACA+CGG - Chr7:95360695-95360714 None:intergenic 30.0%
TATATAGTTCTTCTTCTCAC+GGG - Chr7:95360539-95360558 None:intergenic 30.0%
TGCCAAAGAATCAATACTTA+GGG - Chr7:95359906-95359925 None:intergenic 30.0%
! AACTTTGTTTGAGAGTTTGA+AGG + Chr7:95359691-95359710 MsG0780041805.01.T01:intron 30.0%
! CACAGAGAATATTGCATTTT+GGG + Chr7:95360921-95360940 MsG0780041805.01.T01:intron 30.0%
! TTCTTCTTCGTTTTCTACAA+TGG - Chr7:95360263-95360282 None:intergenic 30.0%
!!! TATTGATTCTTTGGCAGTTT+TGG + Chr7:95359910-95359929 MsG0780041805.01.T01:intron 30.0%
ACCTTTACTCTTAACGAGTT+CGG + Chr7:95359577-95359596 MsG0780041805.01.T01:intron 35.0%
AGAAAAAGTGAAAAAGGTGG+AGG - Chr7:95360782-95360801 None:intergenic 35.0%
ATACTATGAAGGTCCTCATA+AGG + Chr7:95360556-95360575 MsG0780041805.01.T01:CDS 35.0%
ATCTTATAATGCTGTGCTGT+AGG + Chr7:95360458-95360477 MsG0780041805.01.T01:intron 35.0%
CCAAAGAATCAATACTTAGG+GGG - Chr7:95359904-95359923 None:intergenic 35.0%
CTGCCAAAGAATCAATACTT+AGG - Chr7:95359907-95359926 None:intergenic 35.0%
GAGTTTGAAGGAGAAAGTAA+AGG + Chr7:95359703-95359722 MsG0780041805.01.T01:intron 35.0%
GCCAAAGAATCAATACTTAG+GGG - Chr7:95359905-95359924 None:intergenic 35.0%
GTGAGACTCTTTGGTAAATT+TGG + Chr7:95360620-95360639 MsG0780041805.01.T01:CDS 35.0%
TGTCACATATCATTCATCGA+AGG + Chr7:95359519-95359538 MsG0780041805.01.T01:intron 35.0%
TTAACGAGTTCGGATTCAAT+AGG + Chr7:95359587-95359606 MsG0780041805.01.T01:intron 35.0%
TTTGAAGGAGAAAGTAAAGG+AGG + Chr7:95359706-95359725 MsG0780041805.01.T01:intron 35.0%
! GCACAGAGAATATTGCATTT+TGG + Chr7:95360920-95360939 MsG0780041805.01.T01:intron 35.0%
! GGTGTTGAAGATTTCAATCA+GGG + Chr7:95360661-95360680 MsG0780041805.01.T01:intron 35.0%
!! AGGTGGAGGCATATTTTATA+CGG - Chr7:95360768-95360787 None:intergenic 35.0%
!! ATATTGCATTTTGGGTGTCT+CGG + Chr7:95360929-95360948 MsG0780041805.01.T01:CDS 35.0%
!!! AAGGTGACACATGTCATTTT+TGG - Chr7:95359960-95359979 None:intergenic 35.0%
AAGGTTGCAGACGTAAACTT+GGG - Chr7:95360317-95360336 None:intergenic 40.0%
AGCAACATACTTACGTACCT+CGG - Chr7:95360404-95360423 None:intergenic 40.0%
AGGAAGGTTCTGCATCATAT+TGG - Chr7:95360986-95361005 None:intergenic 40.0%
CCCTATCTTTGCAAAAGTTG+CGG + Chr7:95359844-95359863 MsG0780041805.01.T01:intron 40.0%
GACATGTGTCACCTTACTTA+GGG + Chr7:95359965-95359984 MsG0780041805.01.T01:intron 40.0%
GTCCTCATAAGGTGTATGTT+GGG + Chr7:95360567-95360586 MsG0780041805.01.T01:CDS 40.0%
TAAATGGAGCATGCGGTAAA+TGG + Chr7:95359549-95359568 MsG0780041805.01.T01:intron 40.0%
TCCGAACTCGTTAAGAGTAA+AGG - Chr7:95359581-95359600 None:intergenic 40.0%
TGACATGTGTCACCTTACTT+AGG + Chr7:95359964-95359983 MsG0780041805.01.T01:intron 40.0%
TGTTGAGTATCACAGCTTCT+AGG - Chr7:95360340-95360359 None:intergenic 40.0%
TTCCCAACATACACCTTATG+AGG - Chr7:95360572-95360591 None:intergenic 40.0%
! ACCGCAACTTTTGCAAAGAT+AGG - Chr7:95359848-95359867 None:intergenic 40.0%
! ATTTTGTGGTCGAACTCTAG+TGG + Chr7:95360845-95360864 MsG0780041805.01.T01:intron 40.0%
! CCGCAACTTTTGCAAAGATA+GGG - Chr7:95359847-95359866 None:intergenic 40.0%
! CGCAACTTTTGCAAAGATAG+GGG - Chr7:95359846-95359865 None:intergenic 40.0%
! CTGAATTTTCTCGTTCAGGA+AGG - Chr7:95361002-95361021 None:intergenic 40.0%
! CTTGTTCTTGCAGCATTTTG+TGG + Chr7:95360831-95360850 MsG0780041805.01.T01:intron 40.0%
! GCAACTTTTGCAAAGATAGG+GGG - Chr7:95359845-95359864 None:intergenic 40.0%
!! AACTATAATTTTGGCCCCCA+AGG - Chr7:95360002-95360021 None:intergenic 40.0%
!! CCCCCTAAGTATTGATTCTT+TGG + Chr7:95359901-95359920 MsG0780041805.01.T01:intron 40.0%
!! GGGTGTTGAAGATTTCAATC+AGG + Chr7:95360660-95360679 MsG0780041805.01.T01:intron 40.0%
!!! AGTTTTTTTTTGGCCTTGGG+GGG - Chr7:95359936-95359955 None:intergenic 40.0%
!!! CAGTTTTTTTTTGGCCTTGG+GGG - Chr7:95359937-95359956 None:intergenic 40.0%
!!! GCAGTTTTTTTTTGGCCTTG+GGG - Chr7:95359938-95359957 None:intergenic 40.0%
!!! GGCAGTTTTTTTTTGGCCTT+GGG - Chr7:95359939-95359958 None:intergenic 40.0%
!!! TGGCAGTTTTTTTTTGGCCT+TGG - Chr7:95359940-95359959 None:intergenic 40.0%
AAGAGTGAGAAACGTACGAG+AGG + Chr7:95359414-95359433 MsG0780041805.01.T01:intron 45.0%
ACGTACCTCGGTAGATAGAA+CGG - Chr7:95360392-95360411 None:intergenic 45.0%
AGAAACGTACGAGAGGAAGA+GGG + Chr7:95359421-95359440 MsG0780041805.01.T01:intron 45.0%
ATAGTTCTTCTTCTCACGGG+AGG - Chr7:95360536-95360555 None:intergenic 45.0%
CATATCATTCATCGAAGGCC+CGG + Chr7:95359524-95359543 MsG0780041805.01.T01:intron 45.0%
CCTGAACGAGAAAATTCAGC+TGG + Chr7:95361003-95361022 MsG0780041805.01.T01:CDS 45.0%
CGAACTCTAGTGGTTAGAGA+GGG + Chr7:95360855-95360874 MsG0780041805.01.T01:intron 45.0%
CGAGAAAATTCAGCTGGAAG+TGG + Chr7:95361009-95361028 MsG0780041805.01.T01:CDS 45.0%
CTACGAAGAGGAAGAGAAAG+AGG - Chr7:95360155-95360174 None:intergenic 45.0%
CTTCTTCTCTTCACTCCTCA+CGG + Chr7:95360364-95360383 MsG0780041805.01.T01:intron 45.0%
GAAAATTCAGCTGGAAGTGG+AGG + Chr7:95361012-95361031 MsG0780041805.01.T01:CDS 45.0%
GAAAGACAGAGTAGCAGAGT+TGG - Chr7:95360131-95360150 None:intergenic 45.0%
GAAGGTTGCAGACGTAAACT+TGG - Chr7:95360318-95360337 None:intergenic 45.0%
GAGGAGTGAAGAGAAGAAGT+AGG - Chr7:95360363-95360382 None:intergenic 45.0%
GAGTATCCAACTCCAAGTAG+TGG - Chr7:95360178-95360197 None:intergenic 45.0%
GCTGCAAGAACAAGACTAGT+AGG - Chr7:95360825-95360844 None:intergenic 45.0%
GGTCCTCATAAGGTGTATGT+TGG + Chr7:95360566-95360585 MsG0780041805.01.T01:CDS 45.0%
GGTTTCATTGAGGGACAAAG+AGG - Chr7:95360747-95360766 None:intergenic 45.0%
TCGAACTCTAGTGGTTAGAG+AGG + Chr7:95360854-95360873 MsG0780041805.01.T01:intron 45.0%
TGGAAATGTGGCTAGTGTGA+GGG + Chr7:95360640-95360659 MsG0780041805.01.T01:intron 45.0%
TTGCAGACGTAAACTTGGGT+TGG - Chr7:95360313-95360332 None:intergenic 45.0%
TTGGAAATGTGGCTAGTGTG+AGG + Chr7:95360639-95360658 MsG0780041805.01.T01:intron 45.0%
! AACCTCGTAAGTTGAAGCTG+GGG + Chr7:95360284-95360303 MsG0780041805.01.T01:intron 45.0%
! AATCGACGGATGTTGGTTGT+GGG + Chr7:95359386-95359405 MsG0780041805.01.T01:CDS 45.0%
! ACAACCTCGTAAGTTGAAGC+TGG + Chr7:95360282-95360301 MsG0780041805.01.T01:intron 45.0%
! CAACCTCGTAAGTTGAAGCT+GGG + Chr7:95360283-95360302 MsG0780041805.01.T01:intron 45.0%
! CCAGCTGAATTTTCTCGTTC+AGG - Chr7:95361006-95361025 None:intergenic 45.0%
! GAGTATCACAGCTTCTAGGA+AGG - Chr7:95360336-95360355 None:intergenic 45.0%
!! AATATAACTATTTAATTAAT+TGG - Chr7:95359669-95359688 None:intergenic 5.0%
ACCGCATGCTCCATTTAGTC+CGG - Chr7:95359546-95359565 None:intergenic 50.0%
ATGAACGCCGACACAATCGA+CGG + Chr7:95359372-95359391 MsG0780041805.01.T01:CDS 50.0%
ATGCTGTGCTGTAGGTTTGC+CGG + Chr7:95360466-95360485 MsG0780041805.01.T01:intron 50.0%
CCAAAGAGTCTCACGTCTTC+AGG - Chr7:95360614-95360633 None:intergenic 50.0%
CCTGAAGACGTGAGACTCTT+TGG + Chr7:95360611-95360630 MsG0780041805.01.T01:CDS 50.0%
CGCGAGGAGAAAGAAGGATA+AGG - Chr7:95360229-95360248 None:intergenic 50.0%
CTGACGTGTCACCCTAAGTA+AGG - Chr7:95359979-95359998 None:intergenic 50.0%
GAGAAACGTACGAGAGGAAG+AGG + Chr7:95359420-95359439 MsG0780041805.01.T01:intron 50.0%
GTAGATAGAACGGTACCGTG+AGG - Chr7:95360382-95360401 None:intergenic 50.0%
TCCAAGTAGTGGCTACGAAG+AGG - Chr7:95360167-95360186 None:intergenic 50.0%
TCCTCTTCGTAGCCACTACT+TGG + Chr7:95360163-95360182 MsG0780041805.01.T01:intron 50.0%
TCGTAGCCACTACTTGGAGT+TGG + Chr7:95360169-95360188 MsG0780041805.01.T01:intron 50.0%
TCTAGTGGTTAGAGAGGGTG+TGG + Chr7:95360860-95360879 MsG0780041805.01.T01:intron 50.0%
TTCCCCAGCTTCAACTTACG+AGG - Chr7:95360289-95360308 None:intergenic 50.0%
! CAATCGACGGATGTTGGTTG+TGG + Chr7:95359385-95359404 MsG0780041805.01.T01:CDS 50.0%
! CCAACATCCGTCGATTGTGT+CGG - Chr7:95359382-95359401 None:intergenic 50.0%
!! ATTTTGGGTGTCTCGGATGG+AGG + Chr7:95360936-95360955 MsG0780041805.01.T01:CDS 50.0%
!! TGCATTTTGGGTGTCTCGGA+TGG + Chr7:95360933-95360952 MsG0780041805.01.T01:CDS 50.0%
ATGGAGGAAGCTCTCTGCTC+TGG + Chr7:95360952-95360971 MsG0780041805.01.T01:CDS 55.0%
CATCGAAGGCCCGGACTAAA+TGG + Chr7:95359533-95359552 MsG0780041805.01.T01:intron 55.0%
CCCGGACTAAATGGAGCATG+CGG + Chr7:95359542-95359561 MsG0780041805.01.T01:intron 55.0%
CCGACACAATCGACGGATGT+TGG + Chr7:95359379-95359398 MsG0780041805.01.T01:CDS 55.0%
CCGCATGCTCCATTTAGTCC+GGG - Chr7:95359545-95359564 None:intergenic 55.0%
CGGTACCGTTCTATCTACCG+AGG + Chr7:95360384-95360403 MsG0780041805.01.T01:intron 55.0%
CTGTGACGCGAGGAGAAAGA+AGG - Chr7:95360235-95360254 None:intergenic 55.0%
TAGGGTGACACGTCAGCCTT+GGG + Chr7:95359983-95360002 MsG0780041805.01.T01:intron 55.0%
TTAGGGTGACACGTCAGCCT+TGG + Chr7:95359982-95360001 MsG0780041805.01.T01:intron 55.0%
AGGGTGACACGTCAGCCTTG+GGG + Chr7:95359984-95360003 MsG0780041805.01.T01:intron 60.0%
!! TTGGCAGTTTTGGCCCCCCA+AGG + Chr7:95359920-95359939 MsG0780041805.01.T01:intron 60.0%
AATGGACGCGCTGTGACGCG+AGG - Chr7:95360245-95360264 None:intergenic 65.0%
GGGTGACACGTCAGCCTTGG+GGG + Chr7:95359985-95360004 MsG0780041805.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 95359372 95361047 95359372 ID=MsG0780041805.01;Name=MsG0780041805.01
Chr7 mRNA 95359372 95361047 95359372 ID=MsG0780041805.01.T01;Parent=MsG0780041805.01;Name=MsG0780041805.01.T01;_AED=0.63;_eAED=0.64;_QI=0|0|0|0.33|1|1|3|0|116
Chr7 exon 95359372 95359426 95359372 ID=MsG0780041805.01.T01:exon:20984;Parent=MsG0780041805.01.T01
Chr7 exon 95360480 95360653 95360480 ID=MsG0780041805.01.T01:exon:20985;Parent=MsG0780041805.01.T01
Chr7 exon 95360926 95361047 95360926 ID=MsG0780041805.01.T01:exon:20986;Parent=MsG0780041805.01.T01
Chr7 CDS 95359372 95359426 95359372 ID=MsG0780041805.01.T01:cds;Parent=MsG0780041805.01.T01
Chr7 CDS 95360480 95360653 95360480 ID=MsG0780041805.01.T01:cds;Parent=MsG0780041805.01.T01
Chr7 CDS 95360926 95361047 95360926 ID=MsG0780041805.01.T01:cds;Parent=MsG0780041805.01.T01
Gene Sequence

>MsG0780041805.01.T01

ATGAACGCCGACACAATCGACGGATGTTGGTTGTGGGAGAAGAAGAGTGAGAAACGTTTGCCGGTTAAATGTTCTGTTGAGATGAATAATTTAGAGACATTGAACTCACCTCCCGTGAGAAGAAGAACTATATACTATGAAGGTCCTCATAAGGTGTATGTTGGGAATCTCGCTAAGTCTGCTACACCTGAAGACGTGAGACTCTTTGGTAAATTTGGAAATGTGGCTAAGAATATTGCATTTTGGGTGTCTCGGATGGAGGAAGCTCTCTGCTCTGGTATGATGACCAATATGATGCAGAACCTTCCTGAACGAGAAAATTCAGCTGGAAGTGGAGGTGTCCTACTGTAA
Protein sequence

>MsG0780041805.01.T01

MNADTIDGCWLWEKKSEKRLPVKCSVEMNNLETLNSPPVRRRTIYYEGPHKVYVGNLAKSATPEDVRLFGKFGNVAKNIAFWVSRMEEALCSGMMTNMMQNLPERENSAGSGGVLL*