AlfalfaGEDB | MsG0880041830.01

M. sativa cultivar ZhongmuNo.1 / MsG0880041830.01

KEGG: K03879
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880041830.01.T01	RWR91004.1	100	53	0	0	1	53	46	98	1.16E-29	110

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880041830.01.T01	P93401	90.566	53	5	0	1	53	436	488	1.47E-25	99.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880041830.01.T01	A0A3S3N2E9	100.000	53	0	0	1	53	46	98	5.55e-30	110

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048011.01	MsG0880041830.01	0.842932	1.911662e-58	2.943174e-55
MsG0080049160.01	MsG0880041830.01	0.807591	4.689907e-50	2.622474e-47
MsG0180004667.01	MsG0880041830.01	0.843560	1.300243e-58	2.042222e-55
MsG0280010943.01	MsG0880041830.01	-0.801245	9.862761e-49	4.687326e-46
MsG0280009637.01	MsG0880041830.01	0.861321	1.113716e-63	3.139084e-60
MsG0780039222.01	MsG0880041830.01	0.838203	3.305166e-57	4.395144e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 9 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAACAAGGGAGAGGGATA+TGG	0.382329	8:-1541	None:intergenic
GTTCTCAGTTACTCATCAAA+TGG	0.453486	8:+1558	MsG0880041830.01.T01:CDS
TCATATAAAATCCATGTCCT+AGG	0.488956	8:-1454	None:intergenic
AGTAACTGAGAACAAGGGAG+AGG	0.519031	8:-1548	None:intergenic
AACGACTTGTCACGATCCAT+TGG	0.526129	8:-1478	None:intergenic
CAAGGGAGAGGGATATGGAA+AGG	0.532016	8:-1536	None:intergenic
TTGATGAGTAACTGAGAACA+AGG	0.585321	8:-1554	None:intergenic
GTAACTGAGAACAAGGGAGA+GGG	0.641200	8:-1547	None:intergenic
TGATGAGTAACTGAGAACAA+GGG	0.715369	8:-1553	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGGATTTTATATGAACCAA+TGG	+	Chr8:1462-1481	MsG0880041830.01.T01:CDS	25.0%
	TCATATAAAATCCATGTCCT+AGG	-	Chr8:1457-1476	None:intergenic	30.0%
	GTTCTCAGTTACTCATCAAA+TGG	+	Chr8:1558-1577	MsG0880041830.01.T01:CDS	35.0%
	TGATGAGTAACTGAGAACAA+GGG	-	Chr8:1556-1575	None:intergenic	35.0%
	TTGATGAGTAACTGAGAACA+AGG	-	Chr8:1557-1576	None:intergenic	35.0%
!	TTTTTGATACACCTAGGACA+TGG	+	Chr8:1443-1462	MsG0880041830.01.T01:CDS	35.0%
	AACGACTTGTCACGATCCAT+TGG	-	Chr8:1481-1500	None:intergenic	45.0%
	AGTAACTGAGAACAAGGGAG+AGG	-	Chr8:1551-1570	None:intergenic	45.0%
	GTAACTGAGAACAAGGGAGA+GGG	-	Chr8:1550-1569	None:intergenic	45.0%
	CAAGGGAGAGGGATATGGAA+AGG	-	Chr8:1539-1558	None:intergenic	50.0%
	GAGAACAAGGGAGAGGGATA+TGG	-	Chr8:1544-1563	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	1439	1600	1439	ID=MsG0880041830.01;Name=MsG0880041830.01
Chr8	mRNA	1439	1600	1439	ID=MsG0880041830.01.T01;Parent=MsG0880041830.01;Name=MsG0880041830.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|53
Chr8	exon	1439	1600	1439	ID=MsG0880041830.01.T01:exon:8847;Parent=MsG0880041830.01.T01
Chr8	CDS	1439	1600	1439	ID=MsG0880041830.01.T01:cds;Parent=MsG0880041830.01.T01

Gene Sequence	>MsG0880041830.01.T01 ATGTTTTTTGATACACCTAGGACATGGATTTTATATGAACCAATGGATCGTGACAAGTCGTTACTACTAGCAATGACTTCGTCTTTCATTACTTCATCCTTTCCATATCCCTCTCCCTTGTTCTCAGTTACTCATCAAATGGCACTCAGTTCATATCTTTAA
Protein sequence	>MsG0880041830.01.T01 MFFDTPRTWILYEPMDRDKSLLLAMTSSFITSSFPYPSPLFSVTHQMALSSYL*

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