AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010943.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010943.01.T01 MTR_0041s0130 85.714 63 8 1 3 64 4 66 7.13e-32 107
MsG0280010943.01.T01 MTR_1g063380 88.710 62 7 0 3 64 4 65 1.16e-30 112
MsG0280010943.01.T01 MTR_2g095210 86.567 67 6 2 1 64 1 67 1.97e-30 111
MsG0280010943.01.T01 MTR_7g101870 85.714 63 8 1 3 64 4 66 4.59e-29 107
MsG0280010943.01.T01 MTR_6g025300 52.381 63 29 1 3 64 5 67 2.51e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010943.01.T01 AT5G03340 85.484 62 8 1 3 64 4 64 2.61e-28 105
MsG0280010943.01.T01 AT3G09840 84.615 65 8 2 1 64 1 64 1.53e-27 103
MsG0280010943.01.T01 AT3G53230 80.645 62 12 0 3 64 4 65 5.70e-27 102

Find 30 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTTCTCCACTGCGATTC+TGG 0.202960 2:-77400126 MsG0280010943.01.T01:CDS
TTGTCGATGGAATTTGAAAA+TGG 0.308232 2:+77400301 None:intergenic
GGTTTGAATTTCAGGAAATC+AGG 0.325143 2:-77400155 MsG0280010943.01.T01:intron
TCCTCATCAAGGTACTGTTT+TGG 0.346624 2:-77399986 MsG0280010943.01.T01:CDS
ACTTACGAGTCCGATGATTC+AGG 0.364075 2:+77400270 None:intergenic
GAAAAGTGATTAATTTAAAG+AGG 0.378693 2:+77399957 None:intergenic
CTCTGAAACCATGGAAAAGT+TGG 0.382341 2:-77400030 MsG0280010943.01.T01:CDS
TCTGAAACCATGGAAAAGTT+GGG 0.427446 2:-77400029 MsG0280010943.01.T01:CDS
TCATCCACCACAAGGCGGTT+TGG 0.459564 2:+77400089 None:intergenic
TTTCAGGAAATCAGGGAAGA+AGG 0.463432 2:-77400147 MsG0280010943.01.T01:intron
GTAGAGTAGTGAATAAAGAC+AGG 0.492037 2:+77400369 None:intergenic
AAACCGCCTTGTGGTGGATG+AGG 0.495300 2:-77400087 MsG0280010943.01.T01:CDS
TTCCATGGTTTCAGAGTGCA+TGG 0.496237 2:+77400037 None:intergenic
TGCCATGCACTCTGAAACCA+TGG 0.505814 2:-77400039 MsG0280010943.01.T01:CDS
AAAAGTGATTAATTTAAAGA+GGG 0.517648 2:+77399958 None:intergenic
TCAGGTTGAGCCATTGTCGA+TGG 0.539590 2:+77400288 None:intergenic
ATCGCCAAACCGCCTTGTGG+TGG 0.554958 2:-77400093 MsG0280010943.01.T01:CDS
AATGGCTCAACCTGAATCAT+CGG 0.555601 2:-77400280 MsG0280010943.01.T01:CDS
GTTTGAATTTCAGGAAATCA+GGG 0.561660 2:-77400154 MsG0280010943.01.T01:intron
CCGCCTTGTGGTGGATGAGG+CGG 0.565834 2:-77400084 MsG0280010943.01.T01:CDS
TCATCACACAAACAAAGTCA+AGG 0.576116 2:-77400335 MsG0280010943.01.T01:five_prime_UTR
CTGAAACCATGGAAAAGTTG+GGG 0.594559 2:-77400028 MsG0280010943.01.T01:CDS
TGGTGATACTATCCTCATCA+AGG 0.601146 2:-77399997 MsG0280010943.01.T01:CDS
TCCAAAACAGTACCTTGATG+AGG 0.648639 2:+77399985 None:intergenic
CTTACGTTCCAGAATCGCAG+TGG 0.649836 2:+77400118 None:intergenic
GAAATCGCCAAACCGCCTTG+TGG 0.658822 2:-77400096 MsG0280010943.01.T01:CDS
TAACCGCCTCATCCACCACA+AGG 0.671534 2:+77400081 None:intergenic
TAGAGTAGTGAATAAAGACA+GGG 0.689607 2:+77400370 None:intergenic
CCGCCTCATCCACCACAAGG+CGG 0.743300 2:+77400084 None:intergenic
TGATGAGGATAGTATCACCA+CGG 0.747718 2:+77400000 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATGATATTGAAGTTTGT+TGG - Chr2:77400194-77400213 MsG0280010943.01.T01:intron 20.0%
! CGAAAATCGAAAAATTTGAA+TGG + Chr2:77400152-77400171 None:intergenic 25.0%
GTTTGAATTTCAGGAAATCA+GGG - Chr2:77400216-77400235 MsG0280010943.01.T01:intron 30.0%
TAGAGTAGTGAATAAAGACA+GGG + Chr2:77400003-77400022 None:intergenic 30.0%
TTGTCGATGGAATTTGAAAA+TGG + Chr2:77400072-77400091 None:intergenic 30.0%
! TTTTCAAATTCCATCGACAA+TGG - Chr2:77400072-77400091 MsG0280010943.01.T01:CDS 30.0%
!! AGTTTGTTGGTTTGAATTTC+AGG - Chr2:77400207-77400226 MsG0280010943.01.T01:intron 30.0%
GGTTTGAATTTCAGGAAATC+AGG - Chr2:77400215-77400234 MsG0280010943.01.T01:intron 35.0%
GTAGAGTAGTGAATAAAGAC+AGG + Chr2:77400004-77400023 None:intergenic 35.0%
TCATCACACAAACAAAGTCA+AGG - Chr2:77400035-77400054 MsG0280010943.01.T01:CDS 35.0%
TTTGAATGGAAAATCGAACG+CGG + Chr2:77400138-77400157 None:intergenic 35.0%
! TCTGAAACCATGGAAAAGTT+GGG - Chr2:77400341-77400360 MsG0280010943.01.T01:five_prime_UTR 35.0%
AATGGCTCAACCTGAATCAT+CGG - Chr2:77400090-77400109 MsG0280010943.01.T01:CDS 40.0%
CTGAAACCATGGAAAAGTTG+GGG - Chr2:77400342-77400361 MsG0280010943.01.T01:five_prime_UTR 40.0%
TCCAAAACAGTACCTTGATG+AGG + Chr2:77400388-77400407 None:intergenic 40.0%
TGATGAGGATAGTATCACCA+CGG + Chr2:77400373-77400392 None:intergenic 40.0%
TGGTGATACTATCCTCATCA+AGG - Chr2:77400373-77400392 MsG0280010943.01.T01:five_prime_UTR 40.0%
TTTCAGGAAATCAGGGAAGA+AGG - Chr2:77400223-77400242 MsG0280010943.01.T01:intron 40.0%
!! CTCTGAAACCATGGAAAAGT+TGG - Chr2:77400340-77400359 MsG0280010943.01.T01:five_prime_UTR 40.0%
!!! TCCTCATCAAGGTACTGTTT+TGG - Chr2:77400384-77400403 MsG0280010943.01.T01:five_prime_UTR 40.0%
ACTTACGAGTCCGATGATTC+AGG + Chr2:77400103-77400122 None:intergenic 45.0%
TTCCATGGTTTCAGAGTGCA+TGG + Chr2:77400336-77400355 None:intergenic 45.0%
! GAAAAGCCCCAACTTTTCCA+TGG + Chr2:77400351-77400370 None:intergenic 45.0%
CTTACGTTCCAGAATCGCAG+TGG + Chr2:77400255-77400274 None:intergenic 50.0%
GGATTTCTCCACTGCGATTC+TGG - Chr2:77400244-77400263 MsG0280010943.01.T01:intron 50.0%
! GAAAAGTTGGGGCTTTTCCG+TGG - Chr2:77400353-77400372 MsG0280010943.01.T01:five_prime_UTR 50.0%
!! TCAGGTTGAGCCATTGTCGA+TGG + Chr2:77400085-77400104 None:intergenic 50.0%
!! TGCCATGCACTCTGAAACCA+TGG - Chr2:77400331-77400350 MsG0280010943.01.T01:five_prime_UTR 50.0%
GAAATCGCCAAACCGCCTTG+TGG - Chr2:77400274-77400293 MsG0280010943.01.T01:intron 55.0%
TAACCGCCTCATCCACCACA+AGG + Chr2:77400292-77400311 None:intergenic 55.0%
TCATCCACCACAAGGCGGTT+TGG + Chr2:77400284-77400303 None:intergenic 55.0%
!! AAACCGCCTTGTGGTGGATG+AGG - Chr2:77400283-77400302 MsG0280010943.01.T01:exon 55.0%
ATCGCCAAACCGCCTTGTGG+TGG - Chr2:77400277-77400296 MsG0280010943.01.T01:CDS 60.0%
CCGCCTCATCCACCACAAGG+CGG + Chr2:77400289-77400308 None:intergenic 65.0%
!! CCGCCTTGTGGTGGATGAGG+CGG - Chr2:77400286-77400305 MsG0280010943.01.T01:exon 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 77399971 77400421 77399971 ID=MsG0280010943.01;Name=MsG0280010943.01
Chr2 mRNA 77399971 77400421 77399971 ID=MsG0280010943.01.T01;Parent=MsG0280010943.01;Name=MsG0280010943.01.T01;_AED=0.49;_eAED=0.53;_QI=120|1|0.5|1|0|0.5|2|0|72
Chr2 exon 77399971 77400163 77399971 ID=MsG0280010943.01.T01:exon:11465;Parent=MsG0280010943.01.T01
Chr2 exon 77400276 77400421 77400276 ID=MsG0280010943.01.T01:exon:11464;Parent=MsG0280010943.01.T01
Chr2 five_prime_UTR 77400302 77400421 77400302 ID=MsG0280010943.01.T01:five_prime_utr;Parent=MsG0280010943.01.T01
Chr2 CDS 77400276 77400301 77400276 ID=MsG0280010943.01.T01:cds;Parent=MsG0280010943.01.T01
Chr2 CDS 77399971 77400163 77399971 ID=MsG0280010943.01.T01:cds;Parent=MsG0280010943.01.T01
Gene Sequence

>MsG0280010943.01.T01

ATGGCTCAACCTGAATCATCGGACTCGAAATCAGGGAAGAAGGATTTCTCCACTGCGATTCTGGAACGTAAGAAATCGCCAAACCGCCTTGTGGTGGATGAGGCGGTTAACGATGACAACTCTGTTGTTGCCATGCACTCTGAAACCATGGAAAAGTTGGGGCTTTTCCGTGGTGATACTATCCTCATCAAGGTACTGTTTTGGATATACCCTCTTTAA

Protein sequence

>MsG0280010943.01.T01

MAQPESSDSKSGKKDFSTAILERKKSPNRLVVDEAVNDDNSVVAMHSETMEKLGLFRGDTILIKVLFWIYPL*