Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041968.01.T01 | MCH92163.1 | 64.045 | 89 | 32 | 0 | 1 | 89 | 60 | 148 | 6.30E-31 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041968.01.T01 | A0A392MXX8 | 64.045 | 89 | 32 | 0 | 1 | 89 | 60 | 148 | 3.01e-31 | 116 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580027332.01 | MsG0880041968.01 | 0.808510 | 2.988739e-50 | 1.712114e-47 |
| MsG0780039282.01 | MsG0880041968.01 | 0.803030 | 4.234264e-49 | 2.105627e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041968.01.T01 | MTR_1g047870 | 57.955 | 88 | 35 | 1 | 2 | 89 | 368 | 453 | 1.53e-25 | 98.2 |
| MsG0880041968.01.T01 | MTR_7g091110 | 58.537 | 82 | 32 | 1 | 6 | 87 | 767 | 846 | 1.81e-22 | 90.1 |
| MsG0880041968.01.T01 | MTR_1g047910 | 53.488 | 86 | 38 | 1 | 1 | 86 | 784 | 867 | 2.49e-21 | 86.7 |
| MsG0880041968.01.T01 | MTR_7g491190 | 56.471 | 85 | 30 | 2 | 7 | 89 | 741 | 820 | 3.80e-21 | 86.3 |
| MsG0880041968.01.T01 | MTR_7g091200 | 56.471 | 85 | 30 | 2 | 7 | 89 | 760 | 839 | 3.95e-21 | 86.3 |
| MsG0880041968.01.T01 | MTR_2g062360 | 54.023 | 87 | 38 | 1 | 1 | 87 | 518 | 602 | 1.89e-19 | 81.3 |
| MsG0880041968.01.T01 | MTR_7g091140 | 51.765 | 85 | 39 | 1 | 2 | 86 | 784 | 866 | 2.12e-19 | 81.3 |
| MsG0880041968.01.T01 | MTR_7g491180 | 53.086 | 81 | 36 | 1 | 1 | 81 | 783 | 861 | 2.96e-19 | 80.9 |
| MsG0880041968.01.T01 | MTR_7g091190 | 53.086 | 81 | 36 | 1 | 1 | 81 | 783 | 861 | 2.96e-19 | 80.9 |
| MsG0880041968.01.T01 | MTR_1g078490 | 43.678 | 87 | 47 | 1 | 1 | 87 | 762 | 846 | 1.25e-15 | 70.5 |
| MsG0880041968.01.T01 | MTR_1g047920 | 56.250 | 64 | 26 | 1 | 2 | 65 | 141 | 202 | 1.82e-14 | 66.2 |
| MsG0880041968.01.T01 | MTR_7g091550 | 42.529 | 87 | 44 | 2 | 1 | 87 | 784 | 864 | 9.85e-13 | 62.4 |
| MsG0880041968.01.T01 | MTR_7g078640 | 38.372 | 86 | 51 | 1 | 2 | 87 | 736 | 819 | 7.42e-12 | 59.7 |
| MsG0880041968.01.T01 | MTR_4g119140 | 37.931 | 87 | 52 | 1 | 1 | 87 | 634 | 718 | 8.04e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATTCTGCTTCTCCTTT+TGG | 0.166753 | 8:-1947793 | None:intergenic |
| ACGATTGTCCTCTTCTTAAT+AGG | 0.220344 | 8:+1947623 | MsG0880041968.01.T01:CDS |
| AACATATTTATAAGAAAATT+AGG | 0.274588 | 8:-1947577 | None:intergenic |
| ATTAGGAAGCATCTTAAGAT+TGG | 0.352821 | 8:-1947560 | None:intergenic |
| TAAATATGTTGCATCTTAAG+AGG | 0.381806 | 8:+1947590 | MsG0880041968.01.T01:CDS |
| CTTGAAGATTTGCGCATAGA+TGG | 0.421439 | 8:+1947682 | MsG0880041968.01.T01:CDS |
| TTAGGAAGCATCTTAAGATT+GGG | 0.452785 | 8:-1947559 | None:intergenic |
| ATCGACATGTCTCTCCTCAT+TGG | 0.479863 | 8:+1947658 | MsG0880041968.01.T01:CDS |
| ATCCGCAATGTAGCGAGCAT+TGG | 0.523326 | 8:+1947731 | MsG0880041968.01.T01:CDS |
| ACTTTAGAGACCTTTATGGT+TGG | 0.540334 | 8:+1947529 | None:intergenic |
| ATGAGGAGAGACATGTCGAT+TGG | 0.544665 | 8:-1947655 | None:intergenic |
| AGCAAAATCCTATTAAGAAG+AGG | 0.558516 | 8:-1947631 | None:intergenic |
| GGCCAATGCTCGCTACATTG+CGG | 0.621273 | 8:-1947733 | None:intergenic |
| GCAAATCTTCAAGACCAATG+AGG | 0.715298 | 8:-1947672 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATTTATAAGAAAATT+AGG | - | Chr8:1947580-1947599 | None:intergenic | 10.0% |
| ! | TAAATATGTTGCATCTTAAG+AGG | + | Chr8:1947590-1947609 | MsG0880041968.01.T01:CDS | 25.0% |
| !! | TGATATTTTCTGTACAATTC+AGG | - | Chr8:1947712-1947731 | None:intergenic | 25.0% |
| AGCAAAATCCTATTAAGAAG+AGG | - | Chr8:1947634-1947653 | None:intergenic | 30.0% | |
| !! | ATTAGGAAGCATCTTAAGAT+TGG | - | Chr8:1947563-1947582 | None:intergenic | 30.0% |
| !! | TTAGGAAGCATCTTAAGATT+GGG | - | Chr8:1947562-1947581 | None:intergenic | 30.0% |
| ACGATTGTCCTCTTCTTAAT+AGG | + | Chr8:1947623-1947642 | MsG0880041968.01.T01:CDS | 35.0% | |
| !! | CTTTTGTTTCGAGATATTGC+AGG | - | Chr8:1947757-1947776 | None:intergenic | 35.0% |
| !!! | CGAAACAAAAGCGTTTTCAT+TGG | + | Chr8:1947766-1947785 | MsG0880041968.01.T01:CDS | 35.0% |
| CTTGAAGATTTGCGCATAGA+TGG | + | Chr8:1947682-1947701 | MsG0880041968.01.T01:CDS | 40.0% | |
| GCAAATCTTCAAGACCAATG+AGG | - | Chr8:1947675-1947694 | None:intergenic | 40.0% | |
| ! | GTTTTCATTGGAGAGCCAAA+AGG | + | Chr8:1947778-1947797 | MsG0880041968.01.T01:CDS | 40.0% |
| ATCGACATGTCTCTCCTCAT+TGG | + | Chr8:1947658-1947677 | MsG0880041968.01.T01:CDS | 45.0% | |
| ATGAGGAGAGACATGTCGAT+TGG | - | Chr8:1947658-1947677 | None:intergenic | 45.0% | |
| ATCCGCAATGTAGCGAGCAT+TGG | + | Chr8:1947731-1947750 | MsG0880041968.01.T01:CDS | 50.0% | |
| GGCCAATGCTCGCTACATTG+CGG | - | Chr8:1947736-1947755 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 1947544 | 1947813 | 1947544 | ID=MsG0880041968.01;Name=MsG0880041968.01 |
| Chr8 | mRNA | 1947544 | 1947813 | 1947544 | ID=MsG0880041968.01.T01;Parent=MsG0880041968.01;Name=MsG0880041968.01.T01;_AED=0.48;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr8 | exon | 1947544 | 1947813 | 1947544 | ID=MsG0880041968.01.T01:exon:1187;Parent=MsG0880041968.01.T01 |
| Chr8 | CDS | 1947544 | 1947813 | 1947544 | ID=MsG0880041968.01.T01:cds;Parent=MsG0880041968.01.T01 |
| Gene Sequence |
| Protein sequence |
