AlfalfaGEDB | MsG0880042841.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042841.01

KEGG: -
GO: -
PFAM: PF13639

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042841.01.T01	RHN39582.1	89.404	151	16	0	1	151	1	151	3.24E-94	279

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042841.01.T01	P0C035	54.348	46	21	0	53	98	120	165	2.06E-13	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042841.01.T01	A0A396GDU4	89.404	151	16	0	1	151	1	151	1.55e-94	279

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0880042841.01	MsG0880043262.01	0.803437	3.487650e-49	1.752577e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042841.01.T01	MTR_7g117540	60.870	46	18	0	53	98	101	146	1.02e-15	72.4
MsG0880042841.01.T01	MTR_8g063660	50.847	59	29	0	45	103	86	144	1.11e-15	72.8
MsG0880042841.01.T01	MTR_7g116210	54.000	50	23	0	47	96	29	78	2.77e-15	67.8
MsG0880042841.01.T01	MTR_3g078470	29.921	127	73	4	17	139	74	188	2.99e-15	70.1
MsG0880042841.01.T01	MTR_7g116200	54.000	50	23	0	47	96	87	136	3.55e-15	68.9
MsG0880042841.01.T01	MTR_4g022410	40.000	90	46	3	39	128	103	184	1.12e-14	68.2
MsG0880042841.01.T01	MTR_8g093010	60.465	43	17	0	53	95	134	176	2.34e-14	67.8
MsG0880042841.01.T01	MTR_1g043410	53.061	49	23	0	50	98	120	168	4.53e-14	68.2
MsG0880042841.01.T01	MTR_8g066780	58.696	46	19	0	53	98	142	187	8.56e-14	67.8
MsG0880042841.01.T01	MTR_2g044570	53.061	49	23	0	50	98	80	128	2.08e-13	66.2
MsG0880042841.01.T01	MTR_6g007670	40.698	86	46	3	20	104	142	223	2.60e-13	65.5
MsG0880042841.01.T01	MTR_6g007670	39.394	66	34	1	36	101	22	81	5.10e-12	62.0
MsG0880042841.01.T01	MTR_4g120270	44.068	59	33	0	53	111	104	162	3.07e-13	65.5
MsG0880042841.01.T01	MTR_1g070425	37.500	64	39	1	33	96	78	140	3.40e-13	63.9
MsG0880042841.01.T01	MTR_3g091620	39.506	81	41	2	16	96	98	170	3.43e-13	65.9
MsG0880042841.01.T01	MTR_6g007737	43.103	58	33	0	48	105	154	211	4.47e-13	65.5
MsG0880042841.01.T01	MTR_8g092930	58.140	43	18	0	53	95	139	181	4.70e-13	64.3
MsG0880042841.01.T01	MTR_7g116180	42.593	54	31	0	43	96	86	139	4.72e-13	63.5
MsG0880042841.01.T01	MTR_8g023400	45.614	57	31	0	43	99	107	163	5.84e-13	65.1
MsG0880042841.01.T01	MTR_8g092960	58.140	43	18	0	53	95	130	172	5.85e-13	63.9
MsG0880042841.01.T01	MTR_8g092985	58.140	43	18	0	53	95	138	180	5.96e-13	63.9
MsG0880042841.01.T01	MTR_5g082940	50.000	50	25	0	49	98	99	148	6.19e-13	63.2
MsG0880042841.01.T01	MTR_8g092840	58.140	43	18	0	53	95	140	182	6.63e-13	63.9
MsG0880042841.01.T01	MTR_4g106680	45.283	53	29	0	53	105	132	184	7.74e-13	64.7
MsG0880042841.01.T01	MTR_1g074190	51.064	47	23	0	53	99	131	177	8.43e-13	64.7
MsG0880042841.01.T01	MTR_1g100737	46.667	60	30	1	42	99	110	169	9.61e-13	63.2
MsG0880042841.01.T01	MTR_8g092970	38.889	72	41	1	53	121	131	202	1.02e-12	63.5
MsG0880042841.01.T01	MTR_6g007680	37.333	75	40	2	28	101	46	114	1.06e-12	62.0
MsG0880042841.01.T01	MTR_1g026070	41.379	58	34	0	46	103	64	121	1.16e-12	61.6
MsG0880042841.01.T01	MTR_4g064550	48.148	54	27	1	46	99	208	260	1.30e-12	63.9
MsG0880042841.01.T01	MTR_3g078520	35.897	78	49	1	20	96	69	146	1.36e-12	62.0
MsG0880042841.01.T01	MTR_8g092990	55.814	43	19	0	53	95	141	183	1.41e-12	63.2
MsG0880042841.01.T01	MTR_3g114500	44.231	52	29	0	53	104	71	122	1.48e-12	61.2
MsG0880042841.01.T01	MTR_7g116220	28.409	88	58	2	16	103	67	149	1.56e-12	62.4
MsG0880042841.01.T01	MTR_1g070140	40.323	62	37	0	52	113	129	190	2.17e-12	63.5
MsG0880042841.01.T01	MTR_5g009200	49.057	53	27	0	44	96	341	393	2.56e-12	63.5
MsG0880042841.01.T01	MTR_8g099495	42.105	57	33	0	40	96	334	390	2.62e-12	63.5
MsG0880042841.01.T01	MTR_4g129420	46.296	54	28	1	53	106	144	196	2.70e-12	63.5
MsG0880042841.01.T01	MTR_5g009210	59.091	44	18	0	53	96	315	358	2.87e-12	63.2
MsG0880042841.01.T01	MTR_5g009190	49.057	53	27	0	44	96	285	337	2.94e-12	63.2
MsG0880042841.01.T01	MTR_5g034790	44.444	54	30	0	53	106	142	195	3.83e-12	62.8
MsG0880042841.01.T01	MTR_5g026720	33.784	74	43	1	45	112	85	158	4.00e-12	60.8
MsG0880042841.01.T01	MTR_4g064560	46.296	54	28	1	46	99	202	254	4.23e-12	62.4
MsG0880042841.01.T01	MTR_8g073120	39.437	71	40	1	26	96	74	141	6.89e-12	60.8
MsG0880042841.01.T01	MTR_5g026730	33.750	80	52	1	19	97	59	138	7.07e-12	60.5
MsG0880042841.01.T01	MTR_3g094000	39.062	64	39	0	38	101	104	167	9.00e-12	60.1
MsG0880042841.01.T01	MTR_3g095560	33.721	86	49	1	53	130	136	221	9.27e-12	61.2
MsG0880042841.01.T01	MTR_6g087150	48.936	47	24	0	53	99	119	165	1.07e-11	61.6
MsG0880042841.01.T01	MTR_8g009190	52.174	46	22	0	53	98	105	150	1.49e-11	61.2
MsG0880042841.01.T01	MTR_3g078450	34.694	98	53	4	47	139	97	188	1.64e-11	60.1
MsG0880042841.01.T01	MTR_4g093830	39.024	82	38	2	19	98	126	197	1.73e-11	61.2
MsG0880042841.01.T01	MTR_2g436100	50.000	46	23	0	53	98	119	164	1.74e-11	60.8
MsG0880042841.01.T01	MTR_5g030010	41.772	79	38	3	20	98	123	193	1.89e-11	60.8
MsG0880042841.01.T01	MTR_8g099515	43.860	57	32	0	40	96	324	380	1.97e-11	60.8
MsG0880042841.01.T01	MTR_3g071300	43.137	51	29	0	49	99	102	152	2.59e-11	58.9
MsG0880042841.01.T01	MTR_5g064890	39.080	87	44	4	13	98	121	199	3.33e-11	60.1
MsG0880042841.01.T01	MTR_3g086700	46.809	47	25	0	52	98	74	120	3.47e-11	58.9
MsG0880042841.01.T01	MTR_7g073200	42.373	59	31	1	40	98	97	152	4.14e-11	58.5
MsG0880042841.01.T01	MTR_4g063070	41.667	60	33	2	44	101	326	385	4.26e-11	60.1
MsG0880042841.01.T01	MTR_3g012550	39.286	56	34	0	40	95	95	150	4.99e-11	58.2
MsG0880042841.01.T01	MTR_2g090905	41.270	63	30	1	43	98	54	116	5.28e-11	57.0
MsG0880042841.01.T01	MTR_3g100400	43.396	53	30	0	47	99	150	202	5.50e-11	59.7
MsG0880042841.01.T01	MTR_5g024730	34.940	83	46	3	14	95	72	147	5.66e-11	58.5
MsG0880042841.01.T01	MTR_3g086730	47.826	46	24	0	53	98	75	120	7.54e-11	58.5
MsG0880042841.01.T01	MTR_1g492830	42.308	52	30	0	49	100	84	135	7.88e-11	58.5
MsG0880042841.01.T01	MTR_1g090460	49.020	51	26	0	46	96	88	138	8.20e-11	57.4
MsG0880042841.01.T01	MTR_8g027745	30.435	92	58	1	14	105	281	366	8.78e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042841.01.T01	AT1G53820	54.348	46	21	0	53	98	120	165	2.10e-14	68.9
MsG0880042841.01.T01	AT5G43420	38.462	91	50	2	15	99	94	184	2.85e-14	68.9
MsG0880042841.01.T01	AT3G14320	43.284	67	33	1	37	98	67	133	3.39e-14	67.0
MsG0880042841.01.T01	AT1G72310	50.000	46	23	0	53	98	127	172	6.24e-14	67.8
MsG0880042841.01.T01	AT2G17450	40.278	72	41	1	26	95	73	144	1.70e-13	65.1
MsG0880042841.01.T01	AT3G03550	40.909	88	44	2	11	98	125	204	1.71e-13	66.6
MsG0880042841.01.T01	AT5G06490	38.462	78	47	1	19	96	96	172	2.02e-13	65.1
MsG0880042841.01.T01	AT3G18930	36.905	84	51	1	17	98	118	201	2.14e-13	66.6
MsG0880042841.01.T01	AT3G18930	36.905	84	51	1	17	98	118	201	2.14e-13	66.6
MsG0880042841.01.T01	AT3G18930	36.905	84	51	1	17	98	118	201	2.14e-13	66.6
MsG0880042841.01.T01	AT5G40250	45.283	53	29	0	53	105	143	195	2.26e-13	66.6
MsG0880042841.01.T01	AT3G60966	35.106	94	61	0	5	98	14	107	2.98e-13	63.2
MsG0880042841.01.T01	AT1G53010	51.923	52	25	0	49	100	126	177	5.22e-13	63.5
MsG0880042841.01.T01	AT1G04360	46.970	66	33	2	44	107	124	189	7.80e-13	65.1
MsG0880042841.01.T01	AT5G07040	27.928	111	74	1	2	112	49	153	9.33e-13	62.4
MsG0880042841.01.T01	AT4G40070	36.082	97	53	2	41	136	111	199	1.36e-12	64.3
MsG0880042841.01.T01	AT5G05280	36.709	79	49	1	23	101	84	161	1.39e-12	62.4
MsG0880042841.01.T01	AT3G60220	55.102	49	22	0	50	98	114	162	1.40e-12	64.3
MsG0880042841.01.T01	AT2G27940	33.721	86	52	1	11	96	103	183	3.28e-12	62.4
MsG0880042841.01.T01	AT5G01880	45.161	62	30	2	37	98	93	150	3.93e-12	60.8
MsG0880042841.01.T01	AT1G72220	38.750	80	41	3	19	98	151	222	5.38e-12	62.8
MsG0880042841.01.T01	AT5G17600	41.250	80	35	2	19	96	116	185	5.40e-12	62.4
MsG0880042841.01.T01	AT2G17730	51.852	54	25	1	46	98	188	241	5.79e-12	61.6
MsG0880042841.01.T01	AT4G17905	52.174	46	22	0	53	98	181	226	8.99e-12	62.0
MsG0880042841.01.T01	AT4G17905	52.174	46	22	0	53	98	155	200	1.02e-11	61.6
MsG0880042841.01.T01	AT2G20030	51.064	47	23	0	53	99	124	170	1.37e-11	61.6
MsG0880042841.01.T01	AT3G62690	43.396	53	30	0	47	99	107	159	1.56e-11	60.8
MsG0880042841.01.T01	AT5G66070	51.064	47	23	0	52	98	175	221	1.60e-11	60.1
MsG0880042841.01.T01	AT5G66070	51.064	47	23	0	52	98	175	221	1.60e-11	60.1
MsG0880042841.01.T01	AT3G16720	54.545	44	20	0	53	96	119	162	1.72e-11	60.8
MsG0880042841.01.T01	AT2G44578	43.396	53	30	0	46	98	63	115	1.76e-11	58.9
MsG0880042841.01.T01	AT5G53110	44.068	59	33	0	38	96	315	373	1.84e-11	61.2
MsG0880042841.01.T01	AT5G66070	51.064	47	23	0	52	98	199	245	1.85e-11	60.5
MsG0880042841.01.T01	AT3G48030	46.809	47	25	0	53	99	129	175	2.15e-11	60.5
MsG0880042841.01.T01	AT5G57750	48.837	43	22	0	53	95	122	164	2.18e-11	59.7
MsG0880042841.01.T01	AT3G48030	46.809	47	25	0	53	99	207	253	2.69e-11	60.5
MsG0880042841.01.T01	AT4G15975	52.174	46	22	0	53	98	76	121	2.70e-11	59.7
MsG0880042841.01.T01	AT2G47560	46.809	47	25	0	53	99	108	154	2.71e-11	59.7
MsG0880042841.01.T01	AT1G23980	47.368	57	29	1	43	98	133	189	2.85e-11	60.5
MsG0880042841.01.T01	AT2G44581	36.765	68	43	0	46	113	63	130	3.04e-11	58.2
MsG0880042841.01.T01	AT1G32361	33.333	75	46	1	50	124	151	221	3.63e-11	60.1
MsG0880042841.01.T01	AT1G80400	33.333	93	58	2	11	100	310	401	3.68e-11	60.1
MsG0880042841.01.T01	AT4G35480	42.000	50	29	0	46	95	106	155	3.75e-11	58.9
MsG0880042841.01.T01	AT4G30400	51.163	43	21	0	53	95	134	176	3.81e-11	60.5
MsG0880042841.01.T01	AT5G27420	30.682	88	56	2	14	101	90	172	4.98e-11	59.7
MsG0880042841.01.T01	AT4G33565	37.634	93	44	3	30	115	201	286	6.33e-11	59.7
MsG0880042841.01.T01	AT3G55530	54.545	44	19	1	52	95	210	252	6.91e-11	58.9
MsG0880042841.01.T01	AT1G49210	34.021	97	52	1	50	146	132	216	7.09e-11	58.5
MsG0880042841.01.T01	AT4G09100	45.652	46	25	0	53	98	85	130	7.18e-11	57.0
MsG0880042841.01.T01	AT4G28890	48.936	47	24	0	53	99	123	169	8.20e-11	59.3

Find 20 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTATTTCTATGTTGA+TGG	0.275171	8:-14756880	MsG0880042841.01.T01:CDS
CATCAGAGAAACTCTGTATA+AGG	0.357308	8:+14756617	None:intergenic
TAATCTGAATCTACAAAAGT+TGG	0.396213	8:-14756668	MsG0880042841.01.T01:CDS
TCAACCAAAGATTCTTCAAA+AGG	0.400937	8:+14756640	None:intergenic
ATCCTACATGTCCTCTTTGT+AGG	0.420010	8:-14756714	MsG0880042841.01.T01:CDS
CAACTATTGTGAGGAATTTG+AGG	0.453420	8:+14756959	None:intergenic
ATGCTGATTGTATTGGTGCT+TGG	0.457961	8:-14756747	MsG0880042841.01.T01:CDS
TGATATCTATGATTACATAA+AGG	0.505551	8:+14756769	None:intergenic
TGTTTAAGCCAAATTTGTAA+AGG	0.511462	8:-14756811	MsG0880042841.01.T01:CDS
AGATATCATGCTGATTGTAT+TGG	0.513302	8:-14756754	MsG0880042841.01.T01:CDS
ATCTAGTACCACTGAGTCTG+AGG	0.532904	8:-14756563	MsG0880042841.01.T01:CDS
CTCCTACAAAGAGGACATGT+AGG	0.557247	8:+14756712	None:intergenic
CTATGTTGATGGCTCAGTGA+AGG	0.566987	8:-14756869	MsG0880042841.01.T01:CDS
GTAGATTCAGATTAAAACCA+TGG	0.567540	8:+14756677	None:intergenic
TATTGTGAGGAATTTGAGGA+TGG	0.573923	8:+14756963	None:intergenic
AGGAGCAAAATCAATGACCA+TGG	0.640022	8:-14756694	MsG0880042841.01.T01:CDS
TCTAGTACCACTGAGTCTGA+GGG	0.651679	8:-14756562	MsG0880042841.01.T01:CDS
AAAAGCATCAATAAACAACA+TGG	0.656315	8:+14756536	None:intergenic
CTATAAGAGCAACTATTGTG+AGG	0.666771	8:+14756950	None:intergenic
ATGGAAACCCTCAGACTCAG+TGG	0.669383	8:+14756555	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGATATCTATGATTACATAA+AGG	+	Chr8:14756753-14756772	None:intergenic	20.0%
!!!	AAGAATTATTTCTATGTTGA+TGG	-	Chr8:14756639-14756658	MsG0880042841.01.T01:CDS	20.0%
!	TAATCTGAATCTACAAAAGT+TGG	-	Chr8:14756851-14756870	MsG0880042841.01.T01:CDS	25.0%
!	TGTTTAAGCCAAATTTGTAA+AGG	-	Chr8:14756708-14756727	MsG0880042841.01.T01:CDS	25.0%
!	TTGTAAAGGAGAAAAAGTTA+GGG	-	Chr8:14756722-14756741	MsG0880042841.01.T01:CDS	25.0%
!	TTTGTAAAGGAGAAAAAGTT+AGG	-	Chr8:14756721-14756740	MsG0880042841.01.T01:CDS	25.0%
!!	CTTTTTCTCCTTTACAAATT+TGG	+	Chr8:14756719-14756738	None:intergenic	25.0%
	GTAGATTCAGATTAAAACCA+TGG	+	Chr8:14756845-14756864	None:intergenic	30.0%
	TCAACCAAAGATTCTTCAAA+AGG	+	Chr8:14756882-14756901	None:intergenic	30.0%
!	AGATATCATGCTGATTGTAT+TGG	-	Chr8:14756765-14756784	MsG0880042841.01.T01:CDS	30.0%
!!	GAAACCTTTTGAAGAATCTT+TGG	-	Chr8:14756875-14756894	MsG0880042841.01.T01:CDS	30.0%
	CAACTATTGTGAGGAATTTG+AGG	+	Chr8:14756563-14756582	None:intergenic	35.0%
	CATCAGAGAAACTCTGTATA+AGG	+	Chr8:14756905-14756924	None:intergenic	35.0%
	CTATAAGAGCAACTATTGTG+AGG	+	Chr8:14756572-14756591	None:intergenic	35.0%
	TATTGTGAGGAATTTGAGGA+TGG	+	Chr8:14756559-14756578	None:intergenic	35.0%
!	TTGATTTTGCTCCTACAAAG+AGG	+	Chr8:14756819-14756838	None:intergenic	35.0%
	AGGAGCAAAATCAATGACCA+TGG	-	Chr8:14756825-14756844	MsG0880042841.01.T01:CDS	40.0%
	ATCCTACATGTCCTCTTTGT+AGG	-	Chr8:14756805-14756824	MsG0880042841.01.T01:CDS	40.0%
!	ATGCTGATTGTATTGGTGCT+TGG	-	Chr8:14756772-14756791	MsG0880042841.01.T01:CDS	40.0%
	ATCTAGTACCACTGAGTCTG+AGG	-	Chr8:14756956-14756975	MsG0880042841.01.T01:CDS	45.0%
	CTCCTACAAAGAGGACATGT+AGG	+	Chr8:14756810-14756829	None:intergenic	45.0%
	TCTAGTACCACTGAGTCTGA+GGG	-	Chr8:14756957-14756976	MsG0880042841.01.T01:CDS	45.0%
!!	CTATGTTGATGGCTCAGTGA+AGG	-	Chr8:14756650-14756669	MsG0880042841.01.T01:CDS	45.0%
	ATGGAAACCCTCAGACTCAG+TGG	+	Chr8:14756967-14756986	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	14756543	14756998	14756543	ID=MsG0880042841.01;Name=MsG0880042841.01
Chr8	mRNA	14756543	14756998	14756543	ID=MsG0880042841.01.T01;Parent=MsG0880042841.01;Name=MsG0880042841.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|151
Chr8	exon	14756543	14756998	14756543	ID=MsG0880042841.01.T01:exon:5494;Parent=MsG0880042841.01.T01
Chr8	CDS	14756543	14756998	14756543	ID=MsG0880042841.01.T01:cds;Parent=MsG0880042841.01.T01

Gene Sequence

>MsG0880042841.01.T01

ATGACAAGAAAAACCATCCTCAAATTCCTCACAATAGTTGCTCTTATAGCTTATTCATTTCAAGAAAACATAATCAAGACTCTTGATAAACTCTGCAAGAATTATTTCTATGTTGATGGCTCAGTGAAGGAAATAGATCAAAGTGAAAGTGATAGTTGTTCAGTTTGTTTAAGCCAAATTTGTAAAGGAGAAAAAGTTAGGGTATTACCTTTATGTAATCATAGATATCATGCTGATTGTATTGGTGCTTGGTTGAAGAACAATCCTACATGTCCTCTTTGTAGGAGCAAAATCAATGACCATGGTTTTAATCTGAATCTACAAAAGTTGGTGAAACCTTTTGAAGAATCTTTGGTTGACCTTATACAGAGTTTCTCTGATGCACTTGTAACTATTCTTTATACGATACTTCAATCTAGTACCACTGAGTCTGAGGGTTTCCATGTTGTTTATTGA

Protein sequence

>MsG0880042841.01.T01

MTRKTILKFLTIVALIAYSFQENIIKTLDKLCKNYFYVDGSVKEIDQSESDSCSVCLSQICKGEKVRVLPLCNHRYHADCIGAWLKNNPTCPLCRSKINDHGFNLNLQKLVKPFEESLVDLIQSFSDALVTILYTILQSSTTESEGFHVVY*