AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043262.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGCATCTTTCTTTGATTT+TGG 0.121617 8:+21708938 None:intergenic
AGAATATTGTGGAGAGATTA+AGG 0.301540 8:-21707632 MsG0880043262.01.T01:CDS
CTATAAAATGATGATAATAT+TGG 0.318186 8:-21708182 MsG0880043262.01.T01:five_prime_UTR
TGAGCTCAATCTAAAGATAA+TGG 0.346296 8:-21708215 MsG0880043262.01.T01:five_prime_UTR
ACAAGATTGTAAGAATTCTC+TGG 0.392769 8:+21707664 None:intergenic
GCTCCTGTGCTTTGTAACTA+AGG 0.446848 8:+21708415 None:intergenic
GAAAAGTCTCATTCCATTGA+GGG 0.461869 8:+21708263 None:intergenic
CAATCTAAAGATAATGGAAA+AGG 0.492449 8:-21708209 MsG0880043262.01.T01:five_prime_UTR
GTGCTGGATCTAACCCTCAA+TGG 0.522113 8:-21708276 MsG0880043262.01.T01:five_prime_UTR
AGAAAAGTCTCATTCCATTG+AGG 0.522164 8:+21708262 None:intergenic
AAGAGGAGGAGACTGGTGGA+TGG 0.524498 8:-21707463 MsG0880043262.01.T01:CDS
TACAATGAAGAGGAGGAGAC+TGG 0.545609 8:-21707470 MsG0880043262.01.T01:CDS
TTGTAACTAAGGATCACATA+AGG 0.555757 8:+21708426 None:intergenic
CACAAGAGTTCTGTGCAAGA+AGG 0.570141 8:-21708393 MsG0880043262.01.T01:intron
TTAGAAGCAGTGTTTGAAGA+GGG 0.587548 8:-21707694 MsG0880043262.01.T01:intron
GTGAAGGATGAAGAATATTG+TGG 0.592520 8:-21707643 MsG0880043262.01.T01:exon
GATCCTTAGTTACAAAGCAC+AGG 0.599886 8:-21708418 MsG0880043262.01.T01:five_prime_UTR
TGATCAGAGTTACAATGAAG+AGG 0.605643 8:-21707480 MsG0880043262.01.T01:CDS
TTTAGAAGCAGTGTTTGAAG+AGG 0.610475 8:-21707695 MsG0880043262.01.T01:intron
GAATTCTTACAATCTTGTGA+AGG 0.613624 8:-21707659 MsG0880043262.01.T01:five_prime_UTR
GAAGAGGTTGAGACTTGTGG+TGG 0.615235 8:-21707409 MsG0880043262.01.T01:CDS
TCAGAGTTACAATGAAGAGG+AGG 0.621822 8:-21707477 MsG0880043262.01.T01:CDS
AATGAAGAGGAGGAGACTGG+TGG 0.640663 8:-21707467 MsG0880043262.01.T01:CDS
GATTGTAAGAATTCTCTGGA+AGG 0.642476 8:+21707668 None:intergenic
AATGAAGAGGTTGAGACTTG+TGG 0.659471 8:-21707412 MsG0880043262.01.T01:CDS
AATCAAAGAAAGATGCCTCG+TGG 0.675663 8:-21708934 MsG0880043262.01.T01:five_prime_UTR
ATATTGTGGAGAGATTAAGG+TGG 0.788819 8:-21707629 MsG0880043262.01.T01:CDS
ATAACTTCAAGAGTTCCACG+AGG 0.830018 8:+21708919 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAATCAAATTATTTTTC+AGG + Chr8:21707572-21707591 None:intergenic 10.0%
!!! TTTTTTTTTAGAACTAATTA+TGG + Chr8:21708917-21708936 None:intergenic 10.0%
!! CTATAAAATGATGATAATAT+TGG - Chr8:21708099-21708118 MsG0880043262.01.T01:intron 15.0%
!! TAAAATGTACTATCAATTAT+TGG - Chr8:21708764-21708783 MsG0880043262.01.T01:intron 15.0%
!!! AATTGATAGTACATTTTAAT+AGG + Chr8:21708762-21708781 None:intergenic 15.0%
!!! ATTGATAGTACATTTTAATA+GGG + Chr8:21708761-21708780 None:intergenic 15.0%
!! AGAATGAAATATAAGCAAAA+AGG - Chr8:21708249-21708268 MsG0880043262.01.T01:five_prime_UTR 20.0%
!! ATTATATATTCATGCTAAGT+TGG - Chr8:21707602-21707621 MsG0880043262.01.T01:intron 20.0%
!! GAATGAAATATAAACAAAAG+TGG + Chr8:21708326-21708345 None:intergenic 20.0%
!! GTTTAAAAATTTACACCTAA+AGG - Chr8:21708405-21708424 MsG0880043262.01.T01:five_prime_UTR 20.0%
!! TGTAAGTAATTCTATATACT+AGG - Chr8:21707425-21707444 MsG0880043262.01.T01:CDS 20.0%
!! TTGAATTAGAGAATCAATTT+AGG - Chr8:21707685-21707704 MsG0880043262.01.T01:five_prime_UTR 20.0%
!!! AAACATAAGTAGTATTGATT+TGG - Chr8:21707923-21707942 MsG0880043262.01.T01:intron 20.0%
!!! AGTATATTTTGAGAACATTT+AGG - Chr8:21708942-21708961 MsG0880043262.01.T01:five_prime_UTR 20.0%
!!! ATAGTAGACTAAATTTTTAG+CGG - Chr8:21708279-21708298 MsG0880043262.01.T01:five_prime_UTR 20.0%
!!! ATATTTTGATGAGTAATATC+AGG - Chr8:21707983-21708002 MsG0880043262.01.T01:intron 20.0%
!!! CTTTTGTTTATATTTCATTC+CGG - Chr8:21708326-21708345 MsG0880043262.01.T01:intron 20.0%
!!! TTTTTGCTTATATTTCATTC+TGG + Chr8:21708250-21708269 None:intergenic 20.0%
! AAAATTGAAAGTCAAACTCT+TGG + Chr8:21707529-21707548 None:intergenic 25.0%
! TAAAAATTTACACCTAAAGG+AGG - Chr8:21708408-21708427 MsG0880043262.01.T01:five_prime_UTR 25.0%
! TAATTCTATATACTAGGAGA+TGG - Chr8:21707431-21707450 MsG0880043262.01.T01:CDS 25.0%
! TGAAATATAAGCAAAAAGGT+AGG - Chr8:21708253-21708272 MsG0880043262.01.T01:five_prime_UTR 25.0%
!! ACTTTAATCATTGTGTTTTC+TGG - Chr8:21708966-21708985 MsG0880043262.01.T01:five_prime_UTR 25.0%
!! TGTCTTTGATTCATCTTTTT+AGG - Chr8:21708161-21708180 MsG0880043262.01.T01:intron 25.0%
!!! CAATCTAAAGATAATGGAAA+AGG - Chr8:21708072-21708091 MsG0880043262.01.T01:intron 25.0%
!!! TTGATTTTGGAATTGTAGTT+TGG + Chr8:21707333-21707352 None:intergenic 25.0%
!!! TTTGTTATTTTGTTCTCTAG+TGG - Chr8:21707949-21707968 MsG0880043262.01.T01:intron 25.0%
ACAAGATTGTAAGAATTCTC+TGG + Chr8:21708620-21708639 None:intergenic 30.0%
ACTTTAACACTTACTTTCTC+CGG + Chr8:21708348-21708367 None:intergenic 30.0%
AGAATATTGTGGAGAGATTA+AGG - Chr8:21708649-21708668 MsG0880043262.01.T01:intron 30.0%
CAAATTTCTGCATGAAACAA+TGG - Chr8:21707501-21707520 MsG0880043262.01.T01:intron 30.0%
GAATTCTTACAATCTTGTGA+AGG - Chr8:21708622-21708641 MsG0880043262.01.T01:intron 30.0%
GAGAAAGTAAGTGTTAAAGT+TGG - Chr8:21708348-21708367 MsG0880043262.01.T01:intron 30.0%
GTTGGATTCTCATTGAAAAT+TGG - Chr8:21708366-21708385 MsG0880043262.01.T01:intron 30.0%
TGAGCTCAATCTAAAGATAA+TGG - Chr8:21708066-21708085 MsG0880043262.01.T01:intron 30.0%
TTGCTTATATTTCATTCTGG+AGG + Chr8:21708247-21708266 None:intergenic 30.0%
TTGTAACTAAGGATCACATA+AGG + Chr8:21707858-21707877 None:intergenic 30.0%
!! TTATATAGCCTTATTACCTC+AGG + Chr8:21708692-21708711 None:intergenic 30.0%
!!! GTTATTTTGATCAGTGCTAA+AGG - Chr8:21707380-21707399 MsG0880043262.01.T01:exon 30.0%
AAAATTGAACGAGATGTCAG+TGG + Chr8:21708526-21708545 None:intergenic 35.0%
AGAAAAGTCTCATTCCATTG+AGG + Chr8:21708022-21708041 None:intergenic 35.0%
ATATTGTGGAGAGATTAAGG+TGG - Chr8:21708652-21708671 MsG0880043262.01.T01:intron 35.0%
GAAAAGTCTCATTCCATTGA+GGG + Chr8:21708021-21708040 None:intergenic 35.0%
GATTGTAAGAATTCTCTGGA+AGG + Chr8:21708616-21708635 None:intergenic 35.0%
GTGAAGGATGAAGAATATTG+TGG - Chr8:21708638-21708657 MsG0880043262.01.T01:intron 35.0%
TCTTCAAACACTGCTTCTAA+AGG + Chr8:21708587-21708606 None:intergenic 35.0%
TGATCAGAGTTACAATGAAG+AGG - Chr8:21708801-21708820 MsG0880043262.01.T01:intron 35.0%
TTAGAAGCAGTGTTTGAAGA+GGG - Chr8:21708587-21708606 MsG0880043262.01.T01:intron 35.0%
TTTAGAAGCAGTGTTTGAAG+AGG - Chr8:21708586-21708605 MsG0880043262.01.T01:intron 35.0%
! CTTTTACACCTGAGGTAATA+AGG - Chr8:21708681-21708700 MsG0880043262.01.T01:intron 35.0%
!! AATCATTGTGTTTTCTGGTG+TGG - Chr8:21708971-21708990 MsG0880043262.01.T01:five_prime_UTR 35.0%
!! GAGGCATCTTTCTTTGATTT+TGG + Chr8:21707346-21707365 None:intergenic 35.0%
!!! GACTTTTGAAGTGAATGAAG+AGG - Chr8:21708856-21708875 MsG0880043262.01.T01:intron 35.0%
AATCAAAGAAAGATGCCTCG+TGG - Chr8:21707347-21707366 MsG0880043262.01.T01:three_prime_UTR 40.0%
AATGAAGAGGTTGAGACTTG+TGG - Chr8:21708869-21708888 MsG0880043262.01.T01:intron 40.0%
ATAACTTCAAGAGTTCCACG+AGG + Chr8:21707365-21707384 None:intergenic 40.0%
GATCCTTAGTTACAAAGCAC+AGG - Chr8:21707863-21707882 MsG0880043262.01.T01:intron 40.0%
TCAGAGTTACAATGAAGAGG+AGG - Chr8:21708804-21708823 MsG0880043262.01.T01:intron 40.0%
TGATGAGTAATATCAGGTGC+TGG - Chr8:21707989-21708008 MsG0880043262.01.T01:intron 40.0%
! TTTTCCTTTGTGCCTCCTTT+AGG + Chr8:21708423-21708442 None:intergenic 40.0%
CACAAGAGTTCTGTGCAAGA+AGG - Chr8:21707888-21707907 MsG0880043262.01.T01:intron 45.0%
GCTCCTGTGCTTTGTAACTA+AGG + Chr8:21707869-21707888 None:intergenic 45.0%
TACAATGAAGAGGAGGAGAC+TGG - Chr8:21708811-21708830 MsG0880043262.01.T01:intron 45.0%
! TACACCTAAAGGAGGCACAA+AGG - Chr8:21708416-21708435 MsG0880043262.01.T01:five_prime_UTR 45.0%
AATGAAGAGGAGGAGACTGG+TGG - Chr8:21708814-21708833 MsG0880043262.01.T01:intron 50.0%
GAAGAGGTTGAGACTTGTGG+TGG - Chr8:21708872-21708891 MsG0880043262.01.T01:intron 50.0%
GTGCTGGATCTAACCCTCAA+TGG - Chr8:21708005-21708024 MsG0880043262.01.T01:intron 50.0%
!! GGCTCTCACTTTTACACCTG+AGG - Chr8:21708673-21708692 MsG0880043262.01.T01:intron 50.0%
AAGAGGAGGAGACTGGTGGA+TGG - Chr8:21708818-21708837 MsG0880043262.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 21707309 21708994 21707309 ID=MsG0880043262.01;Name=MsG0880043262.01
Chr8 mRNA 21707309 21708994 21707309 ID=MsG0880043262.01.T01;Parent=MsG0880043262.01;Name=MsG0880043262.01.T01;_AED=0.47;_eAED=0.48;_QI=366|0.5|0.6|1|1|1|5|75|58
Chr8 exon 21708878 21708994 21708878 ID=MsG0880043262.01.T01:exon:18081;Parent=MsG0880043262.01.T01
Chr8 exon 21708394 21708462 21708394 ID=MsG0880043262.01.T01:exon:18082;Parent=MsG0880043262.01.T01
Chr8 exon 21708173 21708298 21708173 ID=MsG0880043262.01.T01:exon:18083;Parent=MsG0880043262.01.T01
Chr8 exon 21707609 21707712 21707609 ID=MsG0880043262.01.T01:exon:18084;Parent=MsG0880043262.01.T01
Chr8 exon 21707309 21707510 21707309 ID=MsG0880043262.01.T01:exon:18085;Parent=MsG0880043262.01.T01
Chr8 five_prime_UTR 21708878 21708994 21708878 ID=MsG0880043262.01.T01:five_prime_utr;Parent=MsG0880043262.01.T01
Chr8 five_prime_UTR 21708394 21708462 21708394 ID=MsG0880043262.01.T01:five_prime_utr;Parent=MsG0880043262.01.T01
Chr8 five_prime_UTR 21708173 21708298 21708173 ID=MsG0880043262.01.T01:five_prime_utr;Parent=MsG0880043262.01.T01
Chr8 five_prime_UTR 21707659 21707712 21707659 ID=MsG0880043262.01.T01:five_prime_utr;Parent=MsG0880043262.01.T01
Chr8 CDS 21707609 21707658 21707609 ID=MsG0880043262.01.T01:cds;Parent=MsG0880043262.01.T01
Chr8 CDS 21707384 21707510 21707384 ID=MsG0880043262.01.T01:cds;Parent=MsG0880043262.01.T01
Chr8 three_prime_UTR 21707309 21707383 21707309 ID=MsG0880043262.01.T01:three_prime_utr;Parent=MsG0880043262.01.T01
Gene Sequence

>MsG0880043262.01.T01

ATGAAGAATATTGTGGAGAGATTAAGGTGGCTCTCACTTTTACACCTGAGAGAGATGATGATCAGAGTTACAATGAAGAGGAGGAGACTGGTGGATGGAAAGAATCAGCTAGTGACTTTTGAAGTGAATGAAGAGGTTGAGACTTGTGGTGGATTTACATATTTACAAGATCCATAA

Protein sequence

>MsG0880043262.01.T01

MKNIVERLRWLSLLHLREMMIRVTMKRRRLVDGKNQLVTFEVNEEVETCGGFTYLQDP*