Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043306.01.T01 | XP_013448955.1 | 75.309 | 81 | 7 | 1 | 1 | 68 | 186 | 266 | 6.41E-31 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043306.01.T01 | Q9LHT0 | 56.579 | 76 | 24 | 1 | 2 | 68 | 180 | 255 | 4.39E-22 | 88.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043306.01.T01 | A0A072U041 | 75.309 | 81 | 7 | 1 | 1 | 68 | 186 | 266 | 3.06e-31 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019501.01 | MsG0880043306.01 | 0.808437 | 3.098448e-50 | 1.771662e-47 |
| MsG0480021795.01 | MsG0880043306.01 | 0.802984 | 4.327751e-49 | 2.149549e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043306.01.T01 | MTR_7g063390 | 75.309 | 81 | 7 | 1 | 1 | 68 | 186 | 266 | 7.77e-35 | 119 |
| MsG0880043306.01.T01 | MTR_7g063360 | 71.605 | 81 | 10 | 1 | 1 | 68 | 186 | 266 | 3.87e-32 | 112 |
| MsG0880043306.01.T01 | MTR_7g063260 | 67.089 | 79 | 13 | 1 | 2 | 67 | 187 | 265 | 3.77e-28 | 101 |
| MsG0880043306.01.T01 | MTR_7g063270 | 63.291 | 79 | 17 | 1 | 1 | 67 | 137 | 215 | 6.47e-28 | 99.8 |
| MsG0880043306.01.T01 | MTR_7g063320 | 63.750 | 80 | 16 | 1 | 2 | 68 | 186 | 265 | 1.39e-27 | 100 |
| MsG0880043306.01.T01 | MTR_7g063270 | 63.291 | 79 | 17 | 1 | 1 | 67 | 186 | 264 | 1.96e-27 | 99.8 |
| MsG0880043306.01.T01 | MTR_7g063330 | 66.234 | 77 | 14 | 1 | 3 | 67 | 187 | 263 | 3.17e-27 | 99.0 |
| MsG0880043306.01.T01 | MTR_4g064887 | 57.143 | 77 | 24 | 1 | 1 | 68 | 185 | 261 | 1.28e-22 | 87.0 |
| MsG0880043306.01.T01 | MTR_4g064883 | 50.000 | 76 | 29 | 1 | 2 | 68 | 185 | 260 | 1.81e-18 | 76.3 |
| MsG0880043306.01.T01 | MTR_3g068070 | 51.316 | 76 | 28 | 1 | 2 | 68 | 186 | 261 | 2.18e-18 | 75.9 |
| MsG0880043306.01.T01 | MTR_3g068085 | 51.316 | 76 | 28 | 1 | 2 | 68 | 186 | 261 | 2.38e-18 | 75.9 |
| MsG0880043306.01.T01 | MTR_3g068155 | 49.351 | 77 | 30 | 1 | 1 | 68 | 189 | 265 | 3.94e-18 | 75.5 |
| MsG0880043306.01.T01 | MTR_3g068065 | 52.000 | 75 | 27 | 1 | 2 | 67 | 188 | 262 | 1.16e-17 | 74.3 |
| MsG0880043306.01.T01 | MTR_3g068175 | 50.000 | 76 | 29 | 1 | 2 | 68 | 188 | 263 | 4.28e-17 | 72.8 |
| MsG0880043306.01.T01 | MTR_3g068175 | 50.000 | 76 | 29 | 1 | 2 | 68 | 192 | 267 | 4.85e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043306.01.T01 | AT5G06060 | 56.579 | 76 | 24 | 1 | 2 | 68 | 180 | 255 | 4.47e-23 | 88.2 |
| MsG0880043306.01.T01 | AT2G29310 | 52.632 | 76 | 27 | 1 | 2 | 68 | 176 | 251 | 9.27e-20 | 79.7 |
| MsG0880043306.01.T01 | AT2G30670 | 52.632 | 76 | 27 | 1 | 2 | 68 | 178 | 253 | 1.83e-19 | 79.0 |
| MsG0880043306.01.T01 | AT2G29340 | 51.316 | 76 | 28 | 1 | 2 | 68 | 178 | 253 | 1.99e-19 | 78.6 |
| MsG0880043306.01.T01 | AT2G29320 | 53.947 | 76 | 26 | 2 | 2 | 68 | 167 | 242 | 2.97e-19 | 78.2 |
| MsG0880043306.01.T01 | AT1G07440 | 52.000 | 75 | 28 | 1 | 2 | 68 | 183 | 257 | 3.73e-19 | 78.2 |
| MsG0880043306.01.T01 | AT2G29340 | 51.316 | 76 | 28 | 1 | 2 | 68 | 178 | 253 | 6.51e-19 | 77.8 |
| MsG0880043306.01.T01 | AT2G29330 | 50.649 | 77 | 29 | 1 | 1 | 68 | 177 | 253 | 6.66e-19 | 77.4 |
| MsG0880043306.01.T01 | AT2G29310 | 52.632 | 76 | 27 | 1 | 2 | 68 | 178 | 253 | 8.35e-19 | 77.0 |
| MsG0880043306.01.T01 | AT2G29320 | 53.947 | 76 | 26 | 2 | 2 | 68 | 185 | 260 | 9.72e-19 | 77.0 |
| MsG0880043306.01.T01 | AT1G07450 | 50.649 | 77 | 29 | 1 | 1 | 68 | 177 | 253 | 1.43e-18 | 76.3 |
| MsG0880043306.01.T01 | AT2G29260 | 50.000 | 76 | 29 | 1 | 2 | 68 | 239 | 314 | 2.99e-18 | 76.3 |
| MsG0880043306.01.T01 | AT2G29300 | 52.000 | 75 | 27 | 2 | 2 | 67 | 179 | 253 | 1.54e-17 | 73.9 |
| MsG0880043306.01.T01 | AT2G29300 | 51.316 | 76 | 28 | 2 | 2 | 68 | 179 | 254 | 3.89e-17 | 72.8 |
| MsG0880043306.01.T01 | AT2G29360 | 48.052 | 77 | 31 | 1 | 1 | 68 | 186 | 262 | 6.85e-17 | 72.0 |
| MsG0880043306.01.T01 | AT2G29350 | 48.684 | 76 | 31 | 1 | 1 | 68 | 147 | 222 | 7.81e-17 | 71.2 |
| MsG0880043306.01.T01 | AT2G29290 | 46.753 | 77 | 32 | 2 | 1 | 68 | 160 | 236 | 8.96e-17 | 71.2 |
| MsG0880043306.01.T01 | AT2G29290 | 46.753 | 77 | 32 | 2 | 1 | 68 | 177 | 253 | 1.17e-16 | 71.2 |
| MsG0880043306.01.T01 | AT2G29350 | 48.684 | 76 | 31 | 1 | 1 | 68 | 185 | 260 | 1.57e-16 | 71.2 |
| MsG0880043306.01.T01 | AT2G29370 | 49.351 | 77 | 30 | 1 | 1 | 68 | 186 | 262 | 2.30e-16 | 70.5 |
| MsG0880043306.01.T01 | AT2G29150 | 46.053 | 76 | 32 | 1 | 2 | 68 | 186 | 261 | 6.51e-15 | 66.6 |
Find 19 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGGAGGCTTCACTATTT+AGG | 0.289575 | 8:-22327800 | None:intergenic |
| ACCTGTGATGACCTTACTTT+TGG | 0.297664 | 8:-22328657 | MsG0880043306.01.T01:CDS |
| CGTGCTAATGTTGTGGCTCC+TGG | 0.373198 | 8:-22328680 | MsG0880043306.01.T01:CDS |
| CATAGTAGTCTCCAAAAGTA+AGG | 0.480973 | 8:+22328646 | None:intergenic |
| AGTAAGGTCATCACAGGTCC+AGG | 0.529196 | 8:+22328662 | None:intergenic |
| CAAACACCTAGTGGTCGGAT+TGG | 0.531397 | 8:-22327904 | MsG0880043306.01.T01:CDS |
| ACCTGAGGATATATCAGCAT+TGG | 0.531792 | 8:-22327878 | MsG0880043306.01.T01:CDS |
| ACCAATGCTGATATATCCTC+AGG | 0.536382 | 8:+22327877 | None:intergenic |
| GGTTCTCCAATCCGACCACT+AGG | 0.556909 | 8:+22327898 | None:intergenic |
| TCCAAAAGTAAGGTCATCAC+AGG | 0.566176 | 8:+22328656 | None:intergenic |
| CCAACTGCATCACACATCAC+TGG | 0.570626 | 8:-22327838 | MsG0880043306.01.T01:CDS |
| TAATATTCGTGCTAATGTTG+TGG | 0.584061 | 8:-22328687 | MsG0880043306.01.T01:CDS |
| ACTTAGCATTGGAATGGCAA+AGG | 0.589446 | 8:-22328711 | None:intergenic |
| GGACAAATTATAGCAGCAGA+TGG | 0.590646 | 8:-22327817 | MsG0880043306.01.T01:CDS |
| CAAATTATAGCAGCAGATGG+AGG | 0.609645 | 8:-22327814 | MsG0880043306.01.T01:CDS |
| CCAGTGATGTGTGATGCAGT+TGG | 0.638780 | 8:+22327838 | None:intergenic |
| TGGTCGGATTGGAGAACCTG+AGG | 0.645218 | 8:-22327893 | MsG0880043306.01.T01:CDS |
| TGTCTCAAACACCTAGTGGT+CGG | 0.653560 | 8:-22327909 | MsG0880043306.01.T01:CDS |
| TAGGTGTCTCAAACACCTAG+TGG | 0.670526 | 8:-22327913 | MsG0880043306.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAATTATTTTTTAAAAT+AGG | + | Chr8:22328174-22328193 | None:intergenic | 0.0% |
| !!! | AAAAATATATTTTTTTGTAG+AGG | - | Chr8:22328065-22328084 | MsG0880043306.01.T01:intron | 10.0% |
| !! | AAAATACAAAAGTTCAAATT+AGG | + | Chr8:22328492-22328511 | None:intergenic | 15.0% |
| !! | CATAATTTAGAAATTATGTT+TGG | + | Chr8:22328249-22328268 | None:intergenic | 15.0% |
| !! | TTTCTCTTAAAAAAATTAGT+CGG | - | Chr8:22328405-22328424 | MsG0880043306.01.T01:intron | 15.0% |
| !! | AAATAGGTAATTTAGTTGTT+TGG | + | Chr8:22328158-22328177 | None:intergenic | 20.0% |
| !! | CTTAAAAAAGCTATAACTAA+CGG | + | Chr8:22327934-22327953 | None:intergenic | 20.0% |
| !!! | TTTTTATAGAAATTAGATGC+CGG | - | Chr8:22328543-22328562 | MsG0880043306.01.T01:intron | 20.0% |
| AAATTAGGCCAAATTAGACA+TGG | + | Chr8:22328477-22328496 | None:intergenic | 30.0% | |
| CTAAATTGCCATGTCTAATT+TGG | - | Chr8:22328466-22328485 | MsG0880043306.01.T01:intron | 30.0% | |
| TAATATTCGTGCTAATGTTG+TGG | - | Chr8:22327812-22327831 | MsG0880043306.01.T01:CDS | 30.0% | |
| AAGTGTTCACGTATTGAGAA+GGG | - | Chr8:22328310-22328329 | MsG0880043306.01.T01:intron | 35.0% | |
| CATAGTAGTCTCCAAAAGTA+AGG | + | Chr8:22327856-22327875 | None:intergenic | 35.0% | |
| GTAACAACAGACTCTTAGTT+TGG | - | Chr8:22328275-22328294 | MsG0880043306.01.T01:intron | 35.0% | |
| !! | CTTACTTTTGGAGACTACTA+TGG | - | Chr8:22327854-22327873 | MsG0880043306.01.T01:CDS | 35.0% |
| ACCAATGCTGATATATCCTC+AGG | + | Chr8:22328625-22328644 | None:intergenic | 40.0% | |
| ACCTGAGGATATATCAGCAT+TGG | - | Chr8:22328621-22328640 | MsG0880043306.01.T01:intron | 40.0% | |
| ATAGAAATTAGATGCCGGAG+TGG | - | Chr8:22328548-22328567 | MsG0880043306.01.T01:intron | 40.0% | |
| CAAATTATAGCAGCAGATGG+AGG | - | Chr8:22328685-22328704 | MsG0880043306.01.T01:CDS | 40.0% | |
| CAAGTGTTCACGTATTGAGA+AGG | - | Chr8:22328309-22328328 | MsG0880043306.01.T01:intron | 40.0% | |
| CTTCTCAATACGTGAACACT+TGG | + | Chr8:22328311-22328330 | None:intergenic | 40.0% | |
| GGACAAATTATAGCAGCAGA+TGG | - | Chr8:22328682-22328701 | MsG0880043306.01.T01:CDS | 40.0% | |
| GTGGATATGCTATCAAGGAT+AGG | - | Chr8:22328567-22328586 | MsG0880043306.01.T01:intron | 40.0% | |
| TCCAAAAGTAAGGTCATCAC+AGG | + | Chr8:22327846-22327865 | None:intergenic | 40.0% | |
| ! | ACAGACTCTTAGTTTGGCAA+AGG | - | Chr8:22328281-22328300 | MsG0880043306.01.T01:intron | 40.0% |
| ! | ACCTGTGATGACCTTACTTT+TGG | - | Chr8:22327842-22327861 | MsG0880043306.01.T01:CDS | 40.0% |
| TAGGTGTCTCAAACACCTAG+TGG | - | Chr8:22328586-22328605 | MsG0880043306.01.T01:intron | 45.0% | |
| TGTCTCAAACACCTAGTGGT+CGG | - | Chr8:22328590-22328609 | MsG0880043306.01.T01:intron | 45.0% | |
| ! | CCTTGATAGCATATCCACTC+CGG | + | Chr8:22328565-22328584 | None:intergenic | 45.0% |
| AGTAAGGTCATCACAGGTCC+AGG | + | Chr8:22327840-22327859 | None:intergenic | 50.0% | |
| CAAACACCTAGTGGTCGGAT+TGG | - | Chr8:22328595-22328614 | MsG0880043306.01.T01:intron | 50.0% | |
| CCAACTGCATCACACATCAC+TGG | - | Chr8:22328661-22328680 | MsG0880043306.01.T01:CDS | 50.0% | |
| CCAGTGATGTGTGATGCAGT+TGG | + | Chr8:22328664-22328683 | None:intergenic | 50.0% | |
| CCGGAGTGGATATGCTATCA+AGG | - | Chr8:22328562-22328581 | MsG0880043306.01.T01:intron | 50.0% | |
| ! | CTAACGGACGCTAAGTGAAG+AGG | + | Chr8:22327918-22327937 | None:intergenic | 50.0% |
| CGTGCTAATGTTGTGGCTCC+TGG | - | Chr8:22327819-22327838 | MsG0880043306.01.T01:CDS | 55.0% | |
| GGTTCTCCAATCCGACCACT+AGG | + | Chr8:22328604-22328623 | None:intergenic | 55.0% | |
| ! | TGGTCGGATTGGAGAACCTG+AGG | - | Chr8:22328606-22328625 | MsG0880043306.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 22327801 | 22328720 | 22327801 | ID=MsG0880043306.01;Name=MsG0880043306.01 |
| Chr8 | mRNA | 22327801 | 22328720 | 22327801 | ID=MsG0880043306.01.T01;Parent=MsG0880043306.01;Name=MsG0880043306.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|0.5|2|0|68 |
| Chr8 | exon | 22328646 | 22328720 | 22328646 | ID=MsG0880043306.01.T01:exon:1245;Parent=MsG0880043306.01.T01 |
| Chr8 | exon | 22327801 | 22327932 | 22327801 | ID=MsG0880043306.01.T01:exon:1244;Parent=MsG0880043306.01.T01 |
| Chr8 | CDS | 22328646 | 22328720 | 22328646 | ID=MsG0880043306.01.T01:cds;Parent=MsG0880043306.01.T01 |
| Chr8 | CDS | 22327801 | 22327932 | 22327801 | ID=MsG0880043306.01.T01:cds;Parent=MsG0880043306.01.T01 |
| Gene Sequence |
| Protein sequence |
