AlfalfaGEDB | MsG0480019501.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019501.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019501.01.T01	XP_013455241.1	94.872	117	5	1	1	116	1	117	1.73E-71	219
MsG0480019501.01.T02	XP_013455241.1	93.162	117	8	0	1	117	1	117	2.45E-72	221
MsG0480019501.01.T03	XP_027186811.1	67.857	112	29	2	1	110	1	107	9.42E-44	148

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019501.01.T01	A0A072UHX1	94.872	117	5	1	1	116	1	117	8.27e-72	219
MsG0480019501.01.T02	A0A072UHX1	93.162	117	8	0	1	117	1	117	1.17e-72	221
MsG0480019501.01.T03	A0A3Q7XSI5	67.857	112	29	2	1	110	1	107	4.50e-44	148

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047883.01	MsG0480019501.01	0.825120	5.574000e-54	5.035020e-51
MsG0080047884.01	MsG0480019501.01	0.842469	2.537470e-58	3.851391e-55
MsG0080047885.01	MsG0480019501.01	0.827838	1.254223e-54	1.224944e-51
MsG0080047945.01	MsG0480019501.01	0.807645	4.568735e-50	2.558358e-47
MsG0080047955.01	MsG0480019501.01	0.866398	2.935625e-65	9.880239e-62
MsG0080047956.01	MsG0480019501.01	0.846614	1.944711e-59	3.366396e-56
MsG0080047989.01	MsG0480019501.01	0.836335	9.936945e-57	1.248558e-53
MsG0080048068.01	MsG0480019501.01	0.836633	8.346474e-57	1.057912e-53
MsG0080048191.01	MsG0480019501.01	0.810653	1.035924e-50	6.280370e-48
MsG0080048198.01	MsG0480019501.01	0.829168	5.985806e-55	6.078232e-52

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019501.01.T01	MTR_4g032905	94.872	117	5	1	1	116	1	117	2.10e-75	219
MsG0480019501.01.T02	MTR_4g032905	93.162	117	8	0	1	117	1	117	2.96e-76	221
MsG0480019501.01.T03	MTR_4g032905	93.151	73	4	1	1	72	1	73	5.38e-42	134

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019501.01.T01	AT1G22630	57.627	118	40	2	1	116	1	110	1.24e-39	128
MsG0480019501.01.T02	AT1G22630	55.932	118	43	2	1	117	1	110	9.97e-38	123

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Find 15 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGTAAAAGTTGCGA+AGG	0.564737	4:-21328438	MsG0480019501.01.T03:CDS
AAAGGGGCCATCGAATGTCC+AGG	0.555320	4:-21328414	MsG0480019501.01.T03:CDS
ATACTTCTTGTCAGTGTGAT+TGG	0.374848	4:+21329008	None:intergenic
ATCACTACTTCTGCTTCAAA+AGG	0.440521	4:-21328966	MsG0480019501.01.T03:CDS
ATCTCTGTTTGTTGCTACAA+TGG	0.457545	4:-21329074	MsG0480019501.01.T03:CDS
ATTGTAGCAACAAACAGAGA+TGG	0.617721	4:+21329076	None:intergenic
CATCGAATGTCCAGGATGTA+AGG	0.459912	4:-21328406	MsG0480019501.01.T03:intron
CTTACATCCTGGACATTCGA+TGG	0.580457	4:+21328407	None:intergenic
GTAAAAGTTGCGAAGGCAAA+GGG	0.485475	4:-21328431	MsG0480019501.01.T03:CDS
GTAGCAACAAACAGAGATGG+AGG	0.667885	4:+21329079	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTTGTAAAAGTTGCGA+AGG	-	Chr4:21328236-21328255	MsG0480019501.01.T03:intron	30.0%
ATGATACCAAAGATAATGCA+AGG	+	Chr4:21328492-21328511	None:intergenic	30.0%
TATTAGATATATACACCCTC+CGG	-	Chr4:21328657-21328676	MsG0480019501.01.T03:intron	30.0%
TCTTTATAAAATACACCCTC+CGG	+	Chr4:21328810-21328829	None:intergenic	30.0%
TGATACCAAAGATAATGCAA+GGG	+	Chr4:21328491-21328510	None:intergenic	30.0%
ACTAGAGTTTCCAAACCATA+TGG	-	Chr4:21328004-21328023	MsG0480019501.01.T03:intron	35.0%
ATACTTCTTGTCAGTGTGAT+TGG	+	Chr4:21327669-21327688	None:intergenic	35.0%
ATCACTACTTCTGCTTCAAA+AGG	-	Chr4:21327708-21327727	MsG0480019501.01.T03:CDS	35.0%
ATGGAAATATGTTTGAACGG+TGG	-	Chr4:21328451-21328470	MsG0480019501.01.T03:intron	35.0%
ATTCACACACACTAGTTCAT+AGG	+	Chr4:21329033-21329052	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	21327576	21329120	21327576	ID=MsG0480019501.01;Name=MsG0480019501.01
Chr4	mRNA	21327576	21329120	21327576	ID=MsG0480019501.01.T01;Parent=MsG0480019501.01;Name=MsG0480019501.01.T01;_AED=0.50;_eAED=0.50;_QI=15\|0.33\|0.75\|1\|0\|0\|4\|158\|116
Chr4	five_prime_UTR	21329106	21329120	21329106	ID=MsG0480019501.01.T01:five_prime_utr;Parent=MsG0480019501.01.T01
Chr4	CDS	21328950	21329105	21328950	ID=MsG0480019501.01.T01:cds;Parent=MsG0480019501.01.T01
Chr4	CDS	21328407	21328466	21328407	ID=MsG0480019501.01.T01:cds;Parent=MsG0480019501.01.T01
Chr4	CDS	21328221	21328267	21328221	ID=MsG0480019501.01.T01:cds;Parent=MsG0480019501.01.T01
Chr4	CDS	21327734	21327821	21327734	ID=MsG0480019501.01.T01:cds;Parent=MsG0480019501.01.T01
Chr4	three_prime_UTR	21327576	21327733	21327576	ID=MsG0480019501.01.T01:three_prime_utr;Parent=MsG0480019501.01.T01
Chr4	mRNA	21327576	21329105	21327576	ID=MsG0480019501.01.T02;Parent=MsG0480019501.01;Name=MsG0480019501.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|0.33\|0.5\|1\|0\|0\|4\|158\|117
Chr4	exon	21328407	21328473	21328407	ID=MsG0480019501.01.T02:exon:10326;Parent=MsG0480019501.01.T02
Chr4	exon	21328954	21329105	21328954	ID=MsG0480019501.01.T02:exon:10325;Parent=MsG0480019501.01.T02
Chr4	CDS	21328954	21329105	21328954	ID=MsG0480019501.01.T02:cds;Parent=MsG0480019501.01.T02
Chr4	CDS	21328407	21328473	21328407	ID=MsG0480019501.01.T02:cds;Parent=MsG0480019501.01.T02
Chr4	CDS	21328221	21328267	21328221	ID=MsG0480019501.01.T02:cds;Parent=MsG0480019501.01.T02
Chr4	CDS	21327734	21327821	21327734	ID=MsG0480019501.01.T02:cds;Parent=MsG0480019501.01.T02
Chr4	three_prime_UTR	21327576	21327733	21327576	ID=MsG0480019501.01.T02:three_prime_utr;Parent=MsG0480019501.01.T02
Chr4	mRNA	21327576	21329120	21327576	ID=MsG0480019501.01.T03;Parent=MsG0480019501.01;Name=MsG0480019501.01.T03;_AED=0.50;_eAED=0.50;_QI=15\|0\|0.66\|1\|0\|0\|3\|129\|110
Chr4	five_prime_UTR	21329106	21329120	21329106	ID=MsG0480019501.01.T03:five_prime_utr;Parent=MsG0480019501.01.T03
Chr4	CDS	21328950	21329105	21328950	ID=MsG0480019501.01.T03:cds;Parent=MsG0480019501.01.T03
Chr4	CDS	21328407	21328466	21328407	ID=MsG0480019501.01.T03:cds;Parent=MsG0480019501.01.T03
Chr4	CDS	21327705	21327821	21327705	ID=MsG0480019501.01.T03:cds;Parent=MsG0480019501.01.T03
Chr4	three_prime_UTR	21327576	21327704	21327576	ID=MsG0480019501.01.T03:three_prime_utr;Parent=MsG0480019501.01.T03

Gene Sequence

>MsG0480019501.01.T02

ATGTCCTCCATCTCTGTTTGTTGCTACAATGGTGCTGTGGTTCCACCAACTTTTCTTCATAAAAATCCACTTCAACCAATCACACTGACAAGAAGTATACAACGAAGACAGAGCACAATCACTACTTCTGCTTCAAAAGGATTCTCATTTAATGCATATATTTTTAAAAGTTGTAAAAGTTGCGAAGGCAAAGGGGCCATCGAATGTCCAGGATGTAAGGGATCAGGAAAGAATAAAAAGAATGGAAATATGTTTGAACGGTGGAAATGCTTTGGATGCCAAGGATTTGGGTTGATCAGTTGCCCCAGCTGTGGAAAGGGAGGGCTGACCCCAGAGCAAACAGGAGAAAGATAA

>MsG0480019501.01.T01

ATGTCCTCCATCTCTGTTTGTTGCTACAATGGTGCTGTGGTTCCACCAACTTTTCTTCATAAAAATCCACTTCAACCAATCACACTGACAAGAAGTATACAACGAAGACAGAGCACAATCACTACTTCTGCTTCAAAAGGATTCTCATTTAATTCGATTTTTAAAAGTTGTAAAAGTTGCGAAGGCAAAGGGGCCATCGAATGTCCAGGATGTAAGGGATCAGGAAAGAATAAAAAGAATGGAAATATGTTTGAACGGTGGAAATGCTTTGGATGCCAAGGATTTGGGTTGATCAGTTGCCCCAGCTGTGGAAAGGGAGGGCTGACCCCAGAGCAAACAGGAGAAAGATAA

>MsG0480019501.01.T03

ATGTCCTCCATCTCTGTTTGTTGCTACAATGGTGCTGTGGTTCCACCAACTTTTCTTCATAAAAATCCACTTCAACCAATCACACTGACAAGAAGTATACAACGAAGACAGAGCACAATCACTACTTCTGCTTCAAAAGGATTCTCATTTAATTCGATTTTTAAAAGTTGTAAAAGTTGCGAAGGCAAAGGGGCCATCGAATGTCCAGGATGTAAGATGCTTTGGATGCCAAGGATTTGGGTTGATCAGTTGCCCCAGCTGTGGAAAGGGAGGGCTGACCCCAGAGCAAACAGGAGAAAGATAAAGAAGCTTCAAAACAACCAACTTTTTTAG

Protein sequence

>MsG0480019501.01.T02

MSSISVCCYNGAVVPPTFLHKNPLQPITLTRSIQRRQSTITTSASKGFSFNAYIFKSCKSCEGKGAIECPGCKGSGKNKKNGNMFERWKCFGCQGFGLISCPSCGKGGLTPEQTGER*

>MsG0480019501.01.T01

MSSISVCCYNGAVVPPTFLHKNPLQPITLTRSIQRRQSTITTSASKGFSFNSIFKSCKSCEGKGAIECPGCKGSGKNKKNGNMFERWKCFGCQGFGLISCPSCGKGGLTPEQTGER*

>MsG0480019501.01.T03

MSSISVCCYNGAVVPPTFLHKNPLQPITLTRSIQRRQSTITTSASKGFSFNSIFKSCKSCEGKGAIECPGCKMLWMPRIWVDQLPQLWKGRADPRANRRKIKKLQNNQLF*