AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043333.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043333.01.T01 MTR_8g028500 38.865 229 112 6 31 234 2033 2258 4.03e-39 145
MsG0880043333.01.T01 MTR_8g028500 39.614 207 116 5 29 231 1521 1722 3.11e-36 137
MsG0880043333.01.T01 MTR_8g028500 28.922 204 138 4 31 234 1017 1213 1.27e-18 86.3
MsG0880043333.01.T01 MTR_8g028520 36.123 227 128 6 29 252 1517 1729 2.47e-31 123
MsG0880043333.01.T01 MTR_0006s0240 31.481 216 140 5 31 243 1956 2166 4.02e-28 114
MsG0880043333.01.T01 MTR_0006s0240 29.487 234 149 6 31 257 1513 1737 1.99e-23 100
MsG0880043333.01.T01 MTR_0006s0240 29.064 203 137 2 65 263 1027 1226 1.65e-18 85.9
MsG0880043333.01.T01 MTR_6g463150 33.636 220 131 5 21 234 1528 1738 1.32e-27 112
MsG0880043333.01.T01 MTR_6g463150 32.184 174 111 3 65 234 1056 1226 6.28e-18 84.0
MsG0880043333.01.T01 MTR_0006s0080 31.944 216 139 5 31 243 1369 1579 5.52e-27 110
MsG0880043333.01.T01 MTR_0006s0080 26.437 174 121 2 65 234 883 1053 1.18e-13 71.2
MsG0880043333.01.T01 MTR_8g059275 32.110 218 136 6 31 243 1475 1685 1.62e-26 109
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 563 763 4.57e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 43 243 1.25e-22 97.8
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 955 1155 1.70e-22 97.4
MsG0880043333.01.T01 MTR_8g059275 32.110 218 136 6 31 243 1603 1813 1.76e-26 108
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 563 763 5.61e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 1083 1283 5.61e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 43 243 1.61e-22 97.4
MsG0880043333.01.T01 MTR_1g041550 32.110 218 136 6 31 243 2053 2263 1.81e-26 108
MsG0880043333.01.T01 MTR_1g041550 32.039 206 133 4 31 234 1533 1733 7.35e-22 95.5
MsG0880043333.01.T01 MTR_1g041550 27.586 174 105 3 65 234 1061 1217 1.11e-11 65.5
MsG0880043333.01.T01 MTR_8g059275 32.110 218 136 6 31 243 1607 1817 1.87e-26 108
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 563 763 5.62e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 1083 1283 5.62e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 43 243 1.61e-22 97.4
MsG0880043333.01.T01 MTR_1g044020 33.645 214 137 3 31 243 2323 2532 1.92e-26 108
MsG0880043333.01.T01 MTR_1g044020 30.544 239 159 4 21 257 1811 2044 3.23e-24 102
MsG0880043333.01.T01 MTR_1g044020 30.531 226 150 4 34 257 1299 1519 1.30e-23 100
MsG0880043333.01.T01 MTR_1g044020 30.460 174 114 4 65 234 808 978 1.12e-15 77.4
MsG0880043333.01.T01 MTR_8g059275 32.110 218 136 6 31 243 1995 2205 1.95e-26 108
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 563 763 5.54e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 33.010 206 131 4 31 234 1475 1675 5.54e-23 99.0
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 43 243 1.57e-22 97.4
MsG0880043333.01.T01 MTR_8g059275 32.524 206 132 4 31 234 955 1155 2.15e-22 97.1
MsG0880043333.01.T01 MTR_1g051625 31.140 228 137 6 31 254 548 759 3.52e-26 108
MsG0880043333.01.T01 MTR_1g051625 33.010 206 129 4 31 234 28 226 1.88e-23 100
MsG0880043333.01.T01 MTR_1g036750 29.911 224 150 4 31 252 2036 2254 3.99e-26 108
MsG0880043333.01.T01 MTR_1g036750 33.810 210 132 4 21 228 1506 1710 2.51e-24 102
MsG0880043333.01.T01 MTR_1g036750 31.034 174 113 2 65 234 1030 1200 3.40e-17 82.0
MsG0880043333.01.T01 MTR_1g050395 33.186 226 145 4 20 243 232 453 4.74e-26 107
MsG0880043333.01.T01 MTR_2g089975 31.944 216 139 5 31 243 2585 2795 1.02e-25 107
MsG0880043333.01.T01 MTR_2g089975 34.466 206 128 4 31 234 1546 1746 1.41e-25 106
MsG0880043333.01.T01 MTR_2g089975 30.992 242 155 6 21 257 2056 2290 3.19e-23 99.8
MsG0880043333.01.T01 MTR_2g089975 31.034 174 113 3 65 234 1060 1230 1.46e-15 77.0
MsG0880043333.01.T01 MTR_0069s0080 31.553 206 134 4 31 234 556 756 1.23e-25 106
MsG0880043333.01.T01 MTR_0069s0080 32.524 206 132 4 31 234 1047 1247 2.29e-22 97.1
MsG0880043333.01.T01 MTR_0069s0080 28.879 232 153 6 31 257 34 258 1.05e-19 89.4
MsG0880043333.01.T01 MTR_1g442750 33.010 206 131 4 31 234 1886 2086 1.56e-25 106
MsG0880043333.01.T01 MTR_1g442750 32.212 208 130 5 31 234 1480 1680 2.49e-19 88.2
MsG0880043333.01.T01 MTR_1g037010 31.707 205 134 4 31 234 1471 1670 6.30e-25 104
MsG0880043333.01.T01 MTR_1g037010 29.885 174 115 2 65 234 985 1155 1.95e-16 79.7
MsG0880043333.01.T01 MTR_3g013790 34.021 194 118 4 44 234 1047 1233 1.83e-24 103
MsG0880043333.01.T01 MTR_1g037250 30.556 216 142 5 31 243 1389 1599 3.00e-24 102
MsG0880043333.01.T01 MTR_1g037250 30.460 174 114 2 65 234 982 1152 3.27e-17 82.0
MsG0880043333.01.T01 MTR_0006s0250 29.487 234 149 6 31 257 31 255 1.78e-23 99.4
MsG0880043333.01.T01 MTR_1g037150 29.487 234 149 6 31 257 1534 1758 1.86e-23 100
MsG0880043333.01.T01 MTR_1g037150 29.064 203 137 2 65 263 1048 1247 1.32e-18 85.9
MsG0880043333.01.T01 MTR_6g464030 30.556 216 142 5 31 243 395 605 2.15e-23 99.8
MsG0880043333.01.T01 MTR_2g072150 32.984 191 124 3 46 234 1052 1240 4.05e-23 99.4
MsG0880043333.01.T01 MTR_2g072820 32.124 193 123 4 46 234 1030 1218 4.46e-23 99.0
MsG0880043333.01.T01 MTR_1g036820 30.435 230 152 5 31 257 43 267 4.57e-23 99.0
MsG0880043333.01.T01 MTR_2g071890 30.660 212 128 7 31 234 1023 1223 1.01e-22 98.2
MsG0880043333.01.T01 MTR_0006s0160 33.010 206 131 4 31 234 609 809 1.56e-22 97.4
MsG0880043333.01.T01 MTR_0006s0160 29.885 174 115 2 65 234 133 303 4.78e-17 81.3
MsG0880043333.01.T01 MTR_8g059385 30.508 236 153 5 1 234 1 227 2.55e-22 95.1
MsG0880043333.01.T01 MTR_2g072020 30.366 191 129 3 46 234 1020 1208 3.37e-22 96.7
MsG0880043333.01.T01 MTR_0460s0010 31.250 208 132 5 31 234 18 218 3.66e-22 96.3
MsG0880043333.01.T01 MTR_0460s0010 31.250 208 132 5 31 234 535 735 5.49e-22 95.9
MsG0880043333.01.T01 MTR_8g059325 32.447 188 120 4 31 216 18 200 6.22e-22 90.9
MsG0880043333.01.T01 MTR_1g051585 31.193 218 139 5 21 234 1521 1731 1.08e-21 95.1
MsG0880043333.01.T01 MTR_1g051585 29.885 174 115 2 65 234 1060 1230 7.58e-15 74.7
MsG0880043333.01.T01 MTR_2g071860 32.979 188 118 5 49 234 596 777 1.51e-21 94.7
MsG0880043333.01.T01 MTR_1g036880 30.583 206 136 4 31 234 55 255 1.16e-20 91.7
MsG0880043333.01.T01 MTR_1g050402 36.646 161 98 2 20 180 1555 1711 1.58e-20 91.7
MsG0880043333.01.T01 MTR_1g050402 32.461 191 121 5 48 234 1063 1249 1.53e-13 70.9
MsG0880043333.01.T01 MTR_2g072030 30.000 190 125 4 49 234 1070 1255 3.64e-20 90.5
MsG0880043333.01.T01 MTR_2g072110 29.949 197 130 4 46 238 1060 1252 6.37e-20 89.7
MsG0880043333.01.T01 MTR_2g072110 29.949 197 130 4 46 238 1060 1252 6.77e-20 89.7
MsG0880043333.01.T01 MTR_6g464050 29.310 174 116 2 65 234 1676 1846 2.02e-19 88.6
MsG0880043333.01.T01 MTR_0006s0290 36.196 163 99 3 20 182 20 177 3.81e-19 86.7
MsG0880043333.01.T01 MTR_2g071850 27.604 192 133 3 46 234 1032 1220 5.83e-19 87.0
MsG0880043333.01.T01 MTR_2g071960 27.604 192 133 3 46 234 902 1090 6.50e-19 87.0
MsG0880043333.01.T01 MTR_2g071980 27.604 192 133 3 46 234 1012 1200 8.97e-19 86.7
MsG0880043333.01.T01 MTR_0006s0230 30.645 186 122 2 65 246 1734 1916 1.11e-18 86.3
MsG0880043333.01.T01 MTR_0006s0230 31.034 174 113 2 65 234 1030 1200 2.60e-17 82.0
MsG0880043333.01.T01 MTR_0006s0230 25.483 259 145 5 21 235 1506 1760 3.99e-13 69.7
MsG0880043333.01.T01 MTR_1g037130 30.645 186 122 2 65 246 1763 1945 1.26e-18 86.3
MsG0880043333.01.T01 MTR_1g037130 30.460 174 114 2 65 234 1059 1229 2.49e-16 79.3
MsG0880043333.01.T01 MTR_1g037130 25.483 259 145 5 21 235 1535 1789 4.00e-13 69.7
MsG0880043333.01.T01 MTR_2g072080 32.558 172 109 4 65 234 1004 1170 2.05e-18 85.5
MsG0880043333.01.T01 MTR_2g072090 27.604 192 133 3 46 234 1032 1220 2.72e-18 85.1
MsG0880043333.01.T01 MTR_1g050230 32.867 143 92 2 31 173 268 406 3.15e-18 84.0
MsG0880043333.01.T01 MTR_2g071870 27.604 192 133 3 46 234 1032 1220 3.45e-18 84.7
MsG0880043333.01.T01 MTR_0012s0150 35.526 152 90 3 31 182 1295 1438 1.67e-17 82.8
MsG0880043333.01.T01 MTR_0012s0150 28.736 174 117 2 65 234 809 979 6.81e-15 75.1
MsG0880043333.01.T01 MTR_0068s0080 29.310 174 116 2 65 234 1057 1227 6.31e-17 80.9
MsG0880043333.01.T01 MTR_0068s0080 33.846 130 74 3 21 146 1533 1654 1.14e-11 65.5
MsG0880043333.01.T01 MTR_0068s0080 29.310 174 116 2 65 234 1066 1236 7.14e-17 80.9
MsG0880043333.01.T01 MTR_0006s0300 28.079 203 139 2 65 263 99 298 1.18e-16 80.1
MsG0880043333.01.T01 MTR_0069s0040 29.310 174 116 2 65 234 1060 1230 3.75e-16 78.6
MsG0880043333.01.T01 MTR_1g036800 28.736 174 117 2 65 234 1058 1228 5.09e-16 78.2
MsG0880043333.01.T01 MTR_0002s0030 40.367 109 59 4 1 105 14 120 3.64e-15 71.6
MsG0880043333.01.T01 MTR_4g025430 29.255 188 127 3 49 234 987 1170 4.30e-14 72.8
MsG0880043333.01.T01 MTR_8g059345 27.586 174 119 2 65 234 1058 1228 6.60e-14 72.0
MsG0880043333.01.T01 MTR_8g059345 27.638 199 129 7 46 240 960 1147 8.43e-12 65.9
MsG0880043333.01.T01 MTR_4g025470 29.255 188 127 3 49 234 1020 1203 7.56e-14 72.0
MsG0880043333.01.T01 MTR_1g044095 32.127 221 131 6 8 217 874 1086 7.62e-12 65.9
MsG0880043333.01.T01 MTR_5g036460 28.571 196 123 6 58 240 903 1094 3.90e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTTAACTTTCCATTTC+TGG 0.094377 8:-23139522 MsG0880043333.01.T01:CDS
TTCACACCCTTCCATGATTA+AGG 0.233976 8:+23139763 None:intergenic
CTGGCCTAAGTTGGAAGAAT+TGG 0.278234 8:-23139267 MsG0880043333.01.T01:CDS
CCTTTCTATATCAAAAGATA+TGG 0.296276 8:-23139835 MsG0880043333.01.T01:CDS
TGCCTATTCTAGAGCATGTT+TGG 0.303787 8:-23139562 MsG0880043333.01.T01:CDS
TTCCAGCTTGATTGACCCTT+TGG 0.310946 8:+23139173 None:intergenic
TTGCAGAGACAAGAAGATTC+TGG 0.338350 8:+23139794 None:intergenic
GGATATATGCACCTCAGATT+TGG 0.350620 8:+23139308 None:intergenic
CTGGCGCAACAATAGGAAAT+AGG 0.355414 8:+23139462 None:intergenic
CTGGAGGAAGTATTAATTGA+TGG 0.365000 8:-23139503 MsG0880043333.01.T01:CDS
GCAGCTCTTCCAAGTTACTT+AGG 0.374048 8:+23139699 None:intergenic
CAGGGATAGGCATTCTAAAC+TGG 0.377123 8:-23139286 MsG0880043333.01.T01:CDS
ATTAATACTTCCTCCAGAAA+TGG 0.392580 8:+23139509 None:intergenic
AGACAAGAAGATTCTGGAAT+TGG 0.392802 8:+23139800 None:intergenic
GCATAACATCGTTGAAGTTA+TGG 0.394147 8:-23139337 MsG0880043333.01.T01:CDS
CAGCTCTTCCAAGTTACTTA+GGG 0.398227 8:+23139700 None:intergenic
CAACAAGATCATTTAGTTCT+CGG 0.399695 8:-23139863 MsG0880043333.01.T01:CDS
ATATCCAATTCTTCCAACTT+AGG 0.434892 8:+23139263 None:intergenic
CATACCTTTGCAAGTGAGAC+AGG 0.437944 8:+23139149 None:intergenic
CGAGAACTAAATGATCTTGT+TGG 0.444356 8:+23139864 None:intergenic
CCTCCAGAAATGGAAAGTTA+AGG 0.446261 8:+23139519 None:intergenic
CAATTTAAAGTCCTTAATCA+TGG 0.452105 8:-23139774 MsG0880043333.01.T01:CDS
CTATATCAAAAGATATGGAT+TGG 0.457710 8:-23139830 MsG0880043333.01.T01:CDS
CATTCTAAACTGGCCTAAGT+TGG 0.467716 8:-23139276 MsG0880043333.01.T01:CDS
TGACCCTTTGGATGTGAACC+TGG 0.472908 8:+23139185 None:intergenic
TTCCAAACATGCTCTAGAAT+AGG 0.500008 8:+23139560 None:intergenic
GCCTCCAGGTTCACATCCAA+AGG 0.501674 8:-23139189 MsG0880043333.01.T01:CDS
AATCTGAGGTGCATATATCC+AGG 0.502573 8:-23139305 MsG0880043333.01.T01:CDS
TTAAAGTCCTTAATCATGGA+AGG 0.503802 8:-23139770 MsG0880043333.01.T01:CDS
ATCCAAAGGGTCAATCAAGC+TGG 0.513078 8:-23139175 MsG0880043333.01.T01:CDS
CTCTTCCAAGTTACTTAGGG+AGG 0.517530 8:+23139703 None:intergenic
ACTTCCCTCCCTAAGTAACT+TGG 0.522971 8:-23139708 MsG0880043333.01.T01:CDS
GACTACTTCTACTAATCTAA+AGG 0.526546 8:-23140485 MsG0880043333.01.T01:intron
GTTAGTGGAAATAGTTGCAA+AGG 0.537891 8:-23139393 MsG0880043333.01.T01:CDS
ATGGACAGAGTATCAGAAGC+AGG 0.553960 8:+23139614 None:intergenic
CCCTTTGGATGTGAACCTGG+AGG 0.577987 8:+23139188 None:intergenic
AGGTGCATATATCCAGGGAT+AGG 0.579316 8:-23139299 MsG0880043333.01.T01:CDS
TCTTCCAAGTTACTTAGGGA+GGG 0.580962 8:+23139704 None:intergenic
CAGTTTAGAATGCCTATCCC+TGG 0.587770 8:+23139287 None:intergenic
TAAAGTCCTTAATCATGGAA+GGG 0.596075 8:-23139769 MsG0880043333.01.T01:CDS
TATGGAATTTGCCAAATCTG+AGG 0.597339 8:-23139319 MsG0880043333.01.T01:CDS
GAAACCTGTCTCACTTGCAA+AGG 0.600473 8:-23139153 MsG0880043333.01.T01:intron
CCTTAACTTTCCATTTCTGG+AGG 0.601730 8:-23139519 MsG0880043333.01.T01:CDS
GCAGAAGTTCAAAAGCCTCC+AGG 0.607860 8:-23139203 MsG0880043333.01.T01:CDS
CCTCCAGGTTCACATCCAAA+GGG 0.610974 8:-23139188 MsG0880043333.01.T01:CDS
TAGGGAGGGAAGTATGTGAG+AGG 0.611151 8:+23139718 None:intergenic
ATCTGAGGTGCATATATCCA+GGG 0.617292 8:-23139304 MsG0880043333.01.T01:CDS
AGGGAGGGAAGTATGTGAGA+GGG 0.637708 8:+23139719 None:intergenic
GTTTGGAATGAAGATCTACA+AGG 0.650212 8:-23139545 MsG0880043333.01.T01:CDS
ATGAAGCATCAACCACGTTG+AGG 0.667441 8:-23140507 MsG0880043333.01.T01:CDS
AGTAGAAGTAGTCCTCAACG+TGG 0.817214 8:+23140495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATAAAAAAAT+TGG + Chr8:23139241-23139260 None:intergenic 0.0%
!!! TATTTTATTTTATTTTTTTT+TGG - Chr8:23139248-23139267 MsG0880043333.01.T01:CDS 0.0%
!! AAATAACTAATTTATTTCAA+TGG - Chr8:23139553-23139572 MsG0880043333.01.T01:CDS 10.0%
!!! TCTAATTAATAATATTTTCA+TGG + Chr8:23139516-23139535 None:intergenic 10.0%
!! AAAAAACGAACATATATTAA+AGG + Chr8:23140451-23140470 None:intergenic 15.0%
!! CAAACAAAAATGAATAATAT+GGG - Chr8:23139892-23139911 MsG0880043333.01.T01:intron 15.0%
!! TCAAACAAAAATGAATAATA+TGG - Chr8:23139891-23139910 MsG0880043333.01.T01:intron 15.0%
!! TTAAAATTTGAATTCAAACA+TGG + Chr8:23139290-23139309 None:intergenic 15.0%
!!! TATTTTTTTTTGGAAAAAGA+GGG - Chr8:23139258-23139277 MsG0880043333.01.T01:CDS 15.0%
!!! TTATTTTTTTTTGGAAAAAG+AGG - Chr8:23139257-23139276 MsG0880043333.01.T01:CDS 15.0%
!! TCAAAGTAGTTTAAGAAAAA+TGG - Chr8:23139115-23139134 MsG0880043333.01.T01:intron 20.0%
!! TCAGTTCAATTAATAAATGA+TGG + Chr8:23139081-23139100 None:intergenic 20.0%
!! TCATTTATTAATTGAACTGA+TGG - Chr8:23139081-23139100 MsG0880043333.01.T01:intron 20.0%
!!! TTCAAAATCAGTTTTTTAAG+AGG + Chr8:23139943-23139962 None:intergenic 20.0%
! CCTTTCTATATCAAAAGATA+TGG - Chr8:23139695-23139714 MsG0880043333.01.T01:CDS 25.0%
! CTATATCAAAAGATATGGAT+TGG - Chr8:23139700-23139719 MsG0880043333.01.T01:CDS 25.0%
! GAAAAAATGTAGTAAGTTAG+TGG - Chr8:23140122-23140141 MsG0880043333.01.T01:intron 25.0%
! GAAATATTTCATACTGCAAT+AGG - Chr8:23139424-23139443 MsG0880043333.01.T01:CDS 25.0%
! GAATAGTATGTTATTGAAGA+CGG + Chr8:23140427-23140446 None:intergenic 25.0%
!! CAATTTAAAGTCCTTAATCA+TGG - Chr8:23139756-23139775 MsG0880043333.01.T01:CDS 25.0%
!! CCATATCTTTTGATATAGAA+AGG + Chr8:23139698-23139717 None:intergenic 25.0%
!!! AATCAGTTTTTTAAGAGGAA+TGG + Chr8:23139938-23139957 None:intergenic 25.0%
!!! CTATTTTAAGAAGTTAAGTG+CGG - Chr8:23139185-23139204 MsG0880043333.01.T01:CDS 25.0%
AAACCTTAACTTTCCATTTC+TGG - Chr8:23140008-23140027 MsG0880043333.01.T01:intron 30.0%
ATATCCAATTCTTCCAACTT+AGG + Chr8:23140270-23140289 None:intergenic 30.0%
ATTAATACTTCCTCCAGAAA+TGG + Chr8:23140024-23140043 None:intergenic 30.0%
GACTACTTCTACTAATCTAA+AGG - Chr8:23139045-23139064 MsG0880043333.01.T01:intron 30.0%
TAAAGTCCTTAATCATGGAA+GGG - Chr8:23139761-23139780 MsG0880043333.01.T01:CDS 30.0%
TTAAAGTCCTTAATCATGGA+AGG - Chr8:23139760-23139779 MsG0880043333.01.T01:CDS 30.0%
TTTGATGTATTTACTCTTGC+AGG - Chr8:23139642-23139661 MsG0880043333.01.T01:CDS 30.0%
!! CAACAAGATCATTTAGTTCT+CGG - Chr8:23139667-23139686 MsG0880043333.01.T01:CDS 30.0%
!!! GCTCGATTTTTTTTTTCTTC+CGG - Chr8:23140305-23140324 MsG0880043333.01.T01:intron 30.0%
AGTGCGGATATAGTATGAAT+TGG - Chr8:23139201-23139220 MsG0880043333.01.T01:CDS 35.0%
CGAGAACTAAATGATCTTGT+TGG + Chr8:23139669-23139688 None:intergenic 35.0%
GCATAACATCGTTGAAGTTA+TGG - Chr8:23140193-23140212 MsG0880043333.01.T01:intron 35.0%
GTTAGTGGAAATAGTTGCAA+AGG - Chr8:23140137-23140156 MsG0880043333.01.T01:intron 35.0%
TATGGAATTTGCCAAATCTG+AGG - Chr8:23140211-23140230 MsG0880043333.01.T01:intron 35.0%
! GTTTGGAATGAAGATCTACA+AGG - Chr8:23139985-23140004 MsG0880043333.01.T01:intron 35.0%
! TGTAGTCGATCAATTTTCAC+TGG + Chr8:23140090-23140109 None:intergenic 35.0%
!! AGACAAGAAGATTCTGGAAT+TGG + Chr8:23139733-23139752 None:intergenic 35.0%
!! CTGGAGGAAGTATTAATTGA+TGG - Chr8:23140027-23140046 MsG0880043333.01.T01:intron 35.0%
!! TTCCAAACATGCTCTAGAAT+AGG + Chr8:23139973-23139992 None:intergenic 35.0%
CAGCTCTTCCAAGTTACTTA+GGG + Chr8:23139833-23139852 None:intergenic 40.0%
CCTCCAGAAATGGAAAGTTA+AGG + Chr8:23140014-23140033 None:intergenic 40.0%
GGATATATGCACCTCAGATT+TGG + Chr8:23140225-23140244 None:intergenic 40.0%
TCTTCCAAGTTACTTAGGGA+GGG + Chr8:23139829-23139848 None:intergenic 40.0%
TTCACACCCTTCCATGATTA+AGG + Chr8:23139770-23139789 None:intergenic 40.0%
TTGCAGAGACAAGAAGATTC+TGG + Chr8:23139739-23139758 None:intergenic 40.0%
! AATCTGAGGTGCATATATCC+AGG - Chr8:23140225-23140244 MsG0880043333.01.T01:intron 40.0%
! ATCTGAGGTGCATATATCCA+GGG - Chr8:23140226-23140245 MsG0880043333.01.T01:intron 40.0%
! ATTTTCACTGGCGCAACAAT+AGG + Chr8:23140078-23140097 None:intergenic 40.0%
! CATTCTAAACTGGCCTAAGT+TGG - Chr8:23140254-23140273 MsG0880043333.01.T01:intron 40.0%
! CCTTAACTTTCCATTTCTGG+AGG - Chr8:23140011-23140030 MsG0880043333.01.T01:intron 40.0%
!! TGCCTATTCTAGAGCATGTT+TGG - Chr8:23139968-23139987 MsG0880043333.01.T01:intron 40.0%
ACTTCCCTCCCTAAGTAACT+TGG - Chr8:23139822-23139841 MsG0880043333.01.T01:CDS 45.0%
AGGTGCATATATCCAGGGAT+AGG - Chr8:23140231-23140250 MsG0880043333.01.T01:intron 45.0%
AGTAGAAGTAGTCCTCAACG+TGG + Chr8:23139038-23139057 None:intergenic 45.0%
ATCCAAAGGGTCAATCAAGC+TGG - Chr8:23140355-23140374 MsG0880043333.01.T01:intron 45.0%
CAGGGATAGGCATTCTAAAC+TGG - Chr8:23140244-23140263 MsG0880043333.01.T01:intron 45.0%
CATACCTTTGCAAGTGAGAC+AGG + Chr8:23140384-23140403 None:intergenic 45.0%
CTCTTCCAAGTTACTTAGGG+AGG + Chr8:23139830-23139849 None:intergenic 45.0%
CTGGCGCAACAATAGGAAAT+AGG + Chr8:23140071-23140090 None:intergenic 45.0%
GAAACCTGTCTCACTTGCAA+AGG - Chr8:23140377-23140396 MsG0880043333.01.T01:intron 45.0%
GCAGCTCTTCCAAGTTACTT+AGG + Chr8:23139834-23139853 None:intergenic 45.0%
TGCAAGGCACATAATCGTTG+TGG - Chr8:23139459-23139478 MsG0880043333.01.T01:CDS 45.0%
TTCCAGCTTGATTGACCCTT+TGG + Chr8:23140360-23140379 None:intergenic 45.0%
! ATGAAGCATCAACCACGTTG+AGG - Chr8:23139023-23139042 MsG0880043333.01.T01:CDS 45.0%
! ATGGACAGAGTATCAGAAGC+AGG + Chr8:23139919-23139938 None:intergenic 45.0%
! CAGTTTAGAATGCCTATCCC+TGG + Chr8:23140246-23140265 None:intergenic 45.0%
! CTGGCCTAAGTTGGAAGAAT+TGG - Chr8:23140263-23140282 MsG0880043333.01.T01:intron 45.0%
! TAGGCGTTGCAATTGTTGCA+AGG - Chr8:23139443-23139462 MsG0880043333.01.T01:CDS 45.0%
! TGCTTTTGCAGTCACAGTTG+CGG + Chr8:23139485-23139504 None:intergenic 45.0%
CCTCCAGGTTCACATCCAAA+GGG - Chr8:23140342-23140361 MsG0880043333.01.T01:intron 50.0%
GCAGAAGTTCAAAAGCCTCC+AGG - Chr8:23140327-23140346 MsG0880043333.01.T01:intron 50.0%
TGACCCTTTGGATGTGAACC+TGG + Chr8:23140348-23140367 None:intergenic 50.0%
! AGGGAGGGAAGTATGTGAGA+GGG + Chr8:23139814-23139833 None:intergenic 50.0%
! GGAGGCTTTTGAACTTCTGC+CGG + Chr8:23140327-23140346 None:intergenic 50.0%
! TAGGGAGGGAAGTATGTGAG+AGG + Chr8:23139815-23139834 None:intergenic 50.0%
!! TCGAGACATTTTCGAGCGCT+AGG + Chr8:23139149-23139168 None:intergenic 50.0%
CCCTTTGGATGTGAACCTGG+AGG + Chr8:23140345-23140364 None:intergenic 55.0%
GCCTCCAGGTTCACATCCAA+AGG - Chr8:23140341-23140360 MsG0880043333.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 23139019 23140533 23139019 ID=MsG0880043333.01;Name=MsG0880043333.01
Chr8 mRNA 23139019 23140533 23139019 ID=MsG0880043333.01.T01;Parent=MsG0880043333.01;Name=MsG0880043333.01.T01;_AED=0.38;_eAED=0.40;_QI=0|0|0|0.33|1|1|3|0|272
Chr8 exon 23140486 23140533 23140486 ID=MsG0880043333.01.T01:exon:8440;Parent=MsG0880043333.01.T01
Chr8 exon 23139154 23139888 23139154 ID=MsG0880043333.01.T01:exon:8439;Parent=MsG0880043333.01.T01
Chr8 exon 23139019 23139054 23139019 ID=MsG0880043333.01.T01:exon:8438;Parent=MsG0880043333.01.T01
Chr8 CDS 23140486 23140533 23140486 ID=MsG0880043333.01.T01:cds;Parent=MsG0880043333.01.T01
Chr8 CDS 23139154 23139888 23139154 ID=MsG0880043333.01.T01:cds;Parent=MsG0880043333.01.T01
Chr8 CDS 23139019 23139054 23139019 ID=MsG0880043333.01.T01:cds;Parent=MsG0880043333.01.T01
Gene Sequence

>MsG0880043333.01.T01

ATGAATGAAGCATCAACCACGTTGAGGACTACTTCTACTAATCTAAAGGTCCAACAAGATCATTTAGTTCTCGGTGTTTCCTTTCTATATCAAAAGATATGGATTGGATCAATGCCAATTCCAGAATCTTCTTGTCTCTGCAATTTAAAGTCCTTAATCATGGAAGGGTGTGAATTTTTATCAAATGAAACAATACCCTCTCACATACTTCCCTCCCTAAGTAACTTGGAAGAGCTGCAAGTTCTGAACTGTAACTCTGTTAAGACAATATTCAATCAAACAAAAATGAATAATATGGGACCTGCTTCTGATACTCTGTCCATTCCTCTTAAAAAACTGATTTTGAAACAGTTGCCTATTCTAGAGCATGTTTGGAATGAAGATCTACAAGGAAACCTTAACTTTCCATTTCTGGAGGAAGTATTAATTGATGGATGTGAAAGCATAAAATGCCTATTTCCTATTGTTGCGCCAGTGAAAATTGATCGACTACAAATTCTTCATGTGAAAAAATGTAGTAAGTTAGTGGAAATAGTTGCAAAGGATGCACAAAAAGCAATTGACAAGCTTTTCTCAAGCATAACATCGTTGAAGTTATGGAATTTGCCAAATCTGAGGTGCATATATCCAGGGATAGGCATTCTAAACTGGCCTAAGTTGGAAGAATTGGATATTATACGATGTCAAATGCTCGATTTTTTTTTTCTTCCGGCAGAAGTTCAAAAGCCTCCAGGTTCACATCCAAAGGGTCAATCAAGCTGGAAACCTGTCTCACTTGCAAAGATTGTGACTAGAAGAAATTATGTTGAATCTCGATGA
Protein sequence

>MsG0880043333.01.T01

MNEASTTLRTTSTNLKVQQDHLVLGVSFLYQKIWIGSMPIPESSCLCNLKSLIMEGCEFLSNETIPSHILPSLSNLEELQVLNCNSVKTIFNQTKMNNMGPASDTLSIPLKKLILKQLPILEHVWNEDLQGNLNFPFLEEVLIDGCESIKCLFPIVAPVKIDRLQILHVKKCSKLVEIVAKDAQKAIDKLFSSITSLKLWNLPNLRCIYPGIGILNWPKLEELDIIRCQMLDFFFLPAEVQKPPGSHPKGQSSWKPVSLAKIVTRRNYVESR*