AlfalfaGEDB | MsG0880043612.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043612.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043612.01.T01	XP_024642160.1	48.246	114	58	1	20	132	77	190	2.15E-28	114

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043612.01.T01	A2Q2E7	51.351	111	54	0	25	135	352	462	1.85e-28	118

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0880043612.01	MsG0780041116.01	PPI
MsG0780040274.01	MsG0880043612.01	PPI
MsG0880043612.01	MsG0780041116.01	PPI
MsG0880043612.01	MsG0280009322.01	PPI
MsG0880043612.01	MsG0380013578.01	PPI
MsG0880043612.01	MsG0280010449.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043612.01.T01	MTR_8g098925	42.336	137	62	2	1	137	29	148	3.50e-31	109
MsG0880043612.01.T01	MTR_1g017840	50.000	104	52	0	34	137	58	161	3.34e-30	107
MsG0880043612.01.T01	MTR_6g089560	41.935	124	72	0	14	137	76	199	4.34e-30	108
MsG0880043612.01.T01	MTR_7g013520	48.182	110	57	0	26	135	42	151	4.31e-29	104
MsG0880043612.01.T01	MTR_5g037920	47.664	107	55	1	30	136	74	179	1.05e-27	102
MsG0880043612.01.T01	MTR_8g099580	46.667	105	56	0	33	137	185	289	3.99e-27	102
MsG0880043612.01.T01	MTR_6g453270	46.000	100	54	0	33	132	279	378	1.83e-25	99.4
MsG0880043612.01.T01	MTR_1g069475	39.200	125	74	1	8	132	120	242	3.41e-24	94.0
MsG0880043612.01.T01	MTR_3g116210	39.286	112	68	0	26	137	69	180	1.63e-23	90.5
MsG0880043612.01.T01	MTR_0093s0070	53.086	81	38	0	56	136	125	205	3.31e-23	90.1
MsG0880043612.01.T01	MTR_5g083200	36.496	137	76	2	1	137	56	181	5.00e-23	89.7
MsG0880043612.01.T01	MTR_0002s1220	36.496	137	76	2	1	137	56	181	5.00e-23	89.7
MsG0880043612.01.T01	MTR_4g094272	48.315	89	42	2	16	101	53	140	1.34e-22	87.0
MsG0880043612.01.T01	MTR_3g111390	35.766	137	77	2	1	137	56	181	3.29e-22	87.4
MsG0880043612.01.T01	MTR_1g070820	35.766	137	77	2	1	137	56	181	3.29e-22	87.4
MsG0880043612.01.T01	MTR_8g079540	41.379	116	68	0	7	122	1	116	5.05e-21	82.4
MsG0880043612.01.T01	MTR_6g016265	39.048	105	64	0	33	137	18	122	6.96e-21	82.0
MsG0880043612.01.T01	MTR_1g045340	36.296	135	86	0	3	137	33	167	7.26e-21	83.6
MsG0880043612.01.T01	MTR_1g034430	35.385	130	77	1	8	137	56	178	8.90e-21	83.6
MsG0880043612.01.T01	MTR_5g036320	49.398	83	42	0	33	115	58	140	1.53e-20	81.6
MsG0880043612.01.T01	MTR_6g046670	53.333	75	35	0	33	107	102	176	6.01e-20	81.6
MsG0880043612.01.T01	MTR_5g049680	41.837	98	56	1	33	129	10	107	8.53e-20	79.0
MsG0880043612.01.T01	MTR_1g022215	51.471	68	33	0	41	108	1	68	1.40e-19	77.8
MsG0880043612.01.T01	MTR_1g088650	41.748	103	60	0	35	137	67	169	1.43e-19	80.5
MsG0880043612.01.T01	MTR_5g085210	38.532	109	67	0	29	137	94	202	2.04e-19	80.9
MsG0880043612.01.T01	MTR_2g017550	42.268	97	50	2	17	107	65	161	5.23e-19	78.6
MsG0880043612.01.T01	MTR_2g027020	39.796	98	55	1	33	130	7	100	5.96e-19	79.0
MsG0880043612.01.T01	MTR_5g034450	48.684	76	39	0	40	115	35	110	2.77e-18	75.1
MsG0880043612.01.T01	MTR_1g054905	41.304	92	43	1	6	97	64	144	3.08e-18	76.3
MsG0880043612.01.T01	MTR_8g031800	48.718	78	40	0	36	113	13	90	5.44e-18	74.3
MsG0880043612.01.T01	MTR_1g050488	44.578	83	46	0	38	120	82	164	1.16e-17	74.7
MsG0880043612.01.T01	MTR_6g033225	46.250	80	43	0	40	119	56	135	1.28e-17	73.9
MsG0880043612.01.T01	MTR_2g059470	44.578	83	46	0	55	137	1	83	1.51e-17	72.4
MsG0880043612.01.T01	MTR_4g029360	38.053	113	66	1	29	137	98	210	1.91e-17	75.9
MsG0880043612.01.T01	MTR_5g042170	52.857	70	30	1	29	95	22	91	1.95e-16	70.5
MsG0880043612.01.T01	MTR_7g078610	50.000	66	33	0	31	96	28	93	3.32e-16	69.3
MsG0880043612.01.T01	MTR_8g036780	47.368	76	40	0	33	108	13	88	4.25e-16	69.7
MsG0880043612.01.T01	MTR_1g081930	37.143	105	66	0	33	137	17	121	1.21e-15	68.9
MsG0880043612.01.T01	MTR_3g462490	40.426	94	52	1	1	94	52	141	1.36e-15	72.4
MsG0880043612.01.T01	MTR_8g038340	40.909	88	47	1	26	108	156	243	1.71e-15	70.9
MsG0880043612.01.T01	MTR_0015s0120	53.623	69	32	0	40	108	279	347	1.73e-15	72.0
MsG0880043612.01.T01	MTR_2g047100	39.796	98	44	1	40	137	4	86	1.91e-15	67.4
MsG0880043612.01.T01	MTR_1g074090	47.436	78	41	0	31	108	58	135	6.08e-15	67.4
MsG0880043612.01.T01	MTR_1g018300	37.963	108	60	2	30	137	40	140	6.40e-15	69.3
MsG0880043612.01.T01	MTR_8g031080	46.575	73	38	1	22	93	256	328	2.46e-14	68.9
MsG0880043612.01.T01	MTR_1g099430	42.308	78	43	1	33	108	104	181	5.66e-14	66.2
MsG0880043612.01.T01	MTR_8g022750	30.075	133	74	2	5	137	158	271	6.14e-14	67.0
MsG0880043612.01.T01	MTR_8g059135	38.710	93	53	2	36	128	14	102	6.49e-14	64.3
MsG0880043612.01.T01	MTR_6g016355	40.260	77	46	0	41	117	103	179	1.39e-13	64.7
MsG0880043612.01.T01	MTR_1g109000	42.857	77	44	0	32	108	4	80	1.44e-13	62.4
MsG0880043612.01.T01	MTR_4g008270	39.024	82	50	0	56	137	12	93	6.73e-13	60.8
MsG0880043612.01.T01	MTR_8g045330	46.970	66	35	0	31	96	74	139	8.18e-13	62.0
MsG0880043612.01.T01	MTR_1g074240	43.478	69	39	0	55	123	1	69	9.59e-13	59.7
MsG0880043612.01.T01	MTR_8g028785	34.545	110	47	2	22	130	249	334	1.30e-12	63.9
MsG0880043612.01.T01	MTR_1964s0010	51.064	47	23	0	33	79	48	94	1.61e-12	60.1
MsG0880043612.01.T01	MTR_7g063280	36.905	84	53	0	54	137	72	155	2.57e-12	61.2
MsG0880043612.01.T01	MTR_5g075610	42.308	78	43	1	60	137	1	76	1.90e-11	57.0
MsG0880043612.01.T01	MTR_8g069135	42.857	77	39	1	15	86	150	226	1.91e-11	60.5
MsG0880043612.01.T01	MTR_4g094370	50.909	55	27	0	42	96	23	77	2.25e-11	56.2
MsG0880043612.01.T01	MTR_3g064060	59.091	44	18	0	31	74	14	57	2.80e-11	55.5
MsG0880043612.01.T01	MTR_4g046027	46.032	63	34	0	34	96	107	169	7.03e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTCTAATATTACTGAATT+TGG	0.089882	8:+27761514	MsG0880043612.01.T01:CDS
TGTGGTCGCTGCCAACAAAC+TGG	0.410881	8:-27761235	None:intergenic
CAGGTATGCACCCAGTTTGT+TGG	0.421546	8:+27761224	MsG0880043612.01.T01:CDS
GCAGCGACCACAACATGTTC+AGG	0.453566	8:+27761246	MsG0880043612.01.T01:CDS
CACGAGCAACAAGTTCAATC+AGG	0.470728	8:+27761205	MsG0880043612.01.T01:CDS
TCGGAACAATTGAATATGGC+AGG	0.472714	8:+27761295	MsG0880043612.01.T01:CDS
CAATTGAATATGGCAGGTAT+AGG	0.476145	8:+27761301	MsG0880043612.01.T01:CDS
GATTATATGTGTCGTTAATG+TGG	0.476956	8:+27761381	MsG0880043612.01.T01:CDS
CGTTAATGTGGGTGAAGCCT+TGG	0.498872	8:+27761393	MsG0880043612.01.T01:CDS
TGACCATCGCTTGTCGATGA+AGG	0.506336	8:+27761542	MsG0880043612.01.T01:CDS
ATTATATGTGTCGTTAATGT+GGG	0.510977	8:+27761382	MsG0880043612.01.T01:CDS
TCAACTTCCACAATTTCGAC+TGG	0.525613	8:+27761154	MsG0880043612.01.T01:CDS
ATGTGTATTTGTTATGATGA+TGG	0.527153	8:+27761325	MsG0880043612.01.T01:CDS
AGTAGTACCAGTCGAAATTG+TGG	0.566678	8:-27761161	None:intergenic
CATTTGACCTGAACATGTTG+TGG	0.582511	8:-27761253	None:intergenic
CTTGGATCTCTATTATGCAC+TGG	0.590120	8:+27761411	MsG0880043612.01.T01:CDS
GTGGTCGCTGCCAACAAACT+GGG	0.604370	8:-27761234	None:intergenic
TCACCTTCATCGACAAGCGA+TGG	0.613687	8:-27761545	None:intergenic
ATCTCTATTATGCACTGGAG+TGG	0.636836	8:+27761416	MsG0880043612.01.T01:CDS
CAGTGCATAATAGAGATCCA+AGG	0.655553	8:-27761410	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CGTTCTAATATTACTGAATT+TGG	+	Chr8:27761514-27761533	MsG0880043612.01.T01:CDS	25.0%
!!	ATTATATGTGTCGTTAATGT+GGG	+	Chr8:27761382-27761401	MsG0880043612.01.T01:CDS	25.0%
!!!	ATGTGTATTTGTTATGATGA+TGG	+	Chr8:27761325-27761344	MsG0880043612.01.T01:CDS	25.0%
!!!	TTTTTCGGAACAATTGAATA+TGG	+	Chr8:27761291-27761310	MsG0880043612.01.T01:CDS	25.0%
!	CAATGTTGATGCTTCTTTTT+CGG	+	Chr8:27761276-27761295	MsG0880043612.01.T01:CDS	30.0%
!	GATTATATGTGTCGTTAATG+TGG	+	Chr8:27761381-27761400	MsG0880043612.01.T01:CDS	30.0%
	CAATTGAATATGGCAGGTAT+AGG	+	Chr8:27761301-27761320	MsG0880043612.01.T01:CDS	35.0%
	AGTAGTACCAGTCGAAATTG+TGG	-	Chr8:27761164-27761183	None:intergenic	40.0%
	ATCTCTATTATGCACTGGAG+TGG	+	Chr8:27761416-27761435	MsG0880043612.01.T01:CDS	40.0%
	CAGTGCATAATAGAGATCCA+AGG	-	Chr8:27761413-27761432	None:intergenic	40.0%
	CATTTGACCTGAACATGTTG+TGG	-	Chr8:27761256-27761275	None:intergenic	40.0%
	CTTGGATCTCTATTATGCAC+TGG	+	Chr8:27761411-27761430	MsG0880043612.01.T01:CDS	40.0%
	TCAACTTCCACAATTTCGAC+TGG	+	Chr8:27761154-27761173	MsG0880043612.01.T01:CDS	40.0%
	TCGGAACAATTGAATATGGC+AGG	+	Chr8:27761295-27761314	MsG0880043612.01.T01:CDS	40.0%
	CACGAGCAACAAGTTCAATC+AGG	+	Chr8:27761205-27761224	MsG0880043612.01.T01:CDS	45.0%
	CAGGTATGCACCCAGTTTGT+TGG	+	Chr8:27761224-27761243	MsG0880043612.01.T01:CDS	50.0%
	CGTTAATGTGGGTGAAGCCT+TGG	+	Chr8:27761393-27761412	MsG0880043612.01.T01:CDS	50.0%
	GCAGCGACCACAACATGTTC+AGG	+	Chr8:27761246-27761265	MsG0880043612.01.T01:CDS	55.0%
	GTGGTCGCTGCCAACAAACT+GGG	-	Chr8:27761237-27761256	None:intergenic	55.0%
	TGTGGTCGCTGCCAACAAAC+TGG	-	Chr8:27761238-27761257	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	27761148	27761561	27761148	ID=MsG0880043612.01;Name=MsG0880043612.01
Chr8	mRNA	27761148	27761561	27761148	ID=MsG0880043612.01.T01;Parent=MsG0880043612.01;Name=MsG0880043612.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|137
Chr8	exon	27761148	27761561	27761148	ID=MsG0880043612.01.T01:exon:8786;Parent=MsG0880043612.01.T01
Chr8	CDS	27761148	27761561	27761148	ID=MsG0880043612.01.T01:cds;Parent=MsG0880043612.01.T01

Gene Sequence

>MsG0880043612.01.T01

ATGACTTCAACTTCCACAATTTCGACTGGTACTACTAGCTCGAGTTCTGCTAGTATTCACGAGCAACAAGTTCAATCAGGTATGCACCCAGTTTGTTGGCAGCGACCACAACATGTTCAGGTCAAATGCAATGTTGATGCTTCTTTTTCGGAACAATTGAATATGGCAGGTATAGGTATGTGTATTTGTTATGATGATGGCACTTTTGTTCTTGCTAAAACAATTTCAATTTTGATTATATGTGTCGTTAATGTGGGTGAAGCCTTGGATCTCTATTATGCACTGGAGTGGCTAAGCGACTTGATATTTGACAATGTTGAATTCGTTTCATACTCAAAGCTCATTAAATATGCTTTTAATCATCATCGTTCTAATATTACTGAATTTGGTGAGATGACCATCGCTTGTCGATGA

Protein sequence

>MsG0880043612.01.T01

MTSTSTISTGTTSSSSASIHEQQVQSGMHPVCWQRPQHVQVKCNVDASFSEQLNMAGIGMCICYDDGTFVLAKTISILIICVVNVGEALDLYYALEWLSDLIFDNVEFVSYSKLIKYAFNHHRSNITEFGEMTIACR*