Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043891.01.T01 | XP_024628663.1 | 65.333 | 75 | 26 | 0 | 9 | 83 | 102 | 176 | 3.90E-25 | 103 |
| MsG0880043891.01.T02 | XP_024628663.1 | 60.714 | 112 | 41 | 2 | 1 | 110 | 66 | 176 | 1.49E-38 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043891.01.T01 | A0A2Z6NRS3 | 44.037 | 109 | 33 | 1 | 1 | 81 | 348 | 456 | 9.64e-21 | 95.5 |
| MsG0880043891.01.T02 | A0A2Z6NRS3 | 60.550 | 109 | 42 | 1 | 1 | 108 | 348 | 456 | 2.37e-37 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280010449.01 | MsG0880043891.01 | PPI |
| MsG0780040274.01 | MsG0880043891.01 | PPI |
| MsG0780041116.01 | MsG0880043891.01 | PPI |
| MsG0780041116.01 | MsG0880043891.01 | PPI |
| MsG0380013578.01 | MsG0880043891.01 | PPI |
| MsG0280009322.01 | MsG0880043891.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043891.01.T01 | MTR_5g031620 | 54.386 | 57 | 26 | 0 | 22 | 78 | 139 | 195 | 5.48e-12 | 59.7 |
| MsG0880043891.01.T01 | MTR_5g075610 | 37.000 | 100 | 33 | 2 | 1 | 72 | 1 | 98 | 1.44e-11 | 56.2 |
| MsG0880043891.01.T01 | MTR_8g022750 | 50.000 | 56 | 28 | 0 | 9 | 64 | 230 | 285 | 5.35e-11 | 57.8 |
| MsG0880043891.01.T01 | MTR_7g063280 | 41.667 | 60 | 35 | 0 | 9 | 68 | 114 | 173 | 7.70e-11 | 56.2 |
| MsG0880043891.01.T01 | MTR_7g006380 | 36.585 | 82 | 49 | 2 | 1 | 80 | 72 | 152 | 8.68e-11 | 56.2 |
| MsG0880043891.01.T02 | MTR_7g063280 | 44.211 | 95 | 52 | 1 | 1 | 94 | 78 | 172 | 6.43e-25 | 93.6 |
| MsG0880043891.01.T02 | MTR_5g075610 | 49.000 | 100 | 48 | 2 | 1 | 99 | 1 | 98 | 7.87e-25 | 91.3 |
| MsG0880043891.01.T02 | MTR_5g036320 | 72.881 | 59 | 15 | 1 | 1 | 58 | 85 | 143 | 6.80e-23 | 87.4 |
| MsG0880043891.01.T02 | MTR_2g059470 | 50.000 | 78 | 38 | 1 | 1 | 77 | 6 | 83 | 2.29e-21 | 82.0 |
| MsG0880043891.01.T02 | MTR_3g116210 | 43.182 | 88 | 49 | 1 | 1 | 87 | 103 | 190 | 4.00e-19 | 79.0 |
| MsG0880043891.01.T02 | MTR_6g016265 | 48.148 | 81 | 41 | 1 | 1 | 80 | 45 | 125 | 1.55e-18 | 75.9 |
| MsG0880043891.01.T02 | MTR_8g088220 | 47.297 | 74 | 38 | 1 | 5 | 77 | 4 | 77 | 1.67e-18 | 74.3 |
| MsG0880043891.01.T02 | MTR_5g085210 | 41.509 | 106 | 45 | 2 | 1 | 105 | 125 | 214 | 3.32e-18 | 77.4 |
| MsG0880043891.01.T02 | MTR_1g081930 | 45.783 | 83 | 44 | 1 | 1 | 82 | 44 | 126 | 3.98e-18 | 75.1 |
| MsG0880043891.01.T02 | MTR_2g027020 | 41.593 | 113 | 61 | 2 | 2 | 113 | 35 | 143 | 4.75e-18 | 76.3 |
| MsG0880043891.01.T02 | MTR_8g098925 | 41.304 | 92 | 53 | 1 | 1 | 91 | 71 | 162 | 9.41e-18 | 74.7 |
| MsG0880043891.01.T02 | MTR_1g069475 | 49.315 | 73 | 36 | 1 | 2 | 73 | 171 | 243 | 1.05e-17 | 76.6 |
| MsG0880043891.01.T02 | MTR_6g034915 | 38.889 | 108 | 48 | 2 | 1 | 107 | 61 | 151 | 2.05e-17 | 74.3 |
| MsG0880043891.01.T02 | MTR_6g453270 | 45.205 | 73 | 39 | 1 | 1 | 72 | 306 | 378 | 7.96e-17 | 75.5 |
| MsG0880043891.01.T02 | MTR_1g050488 | 51.667 | 60 | 28 | 1 | 2 | 60 | 105 | 164 | 6.96e-16 | 70.1 |
| MsG0880043891.01.T02 | MTR_6g089560 | 43.373 | 83 | 46 | 1 | 1 | 82 | 122 | 204 | 8.13e-16 | 71.2 |
| MsG0880043891.01.T02 | MTR_5g083200 | 44.828 | 87 | 47 | 1 | 1 | 86 | 104 | 190 | 1.31e-15 | 70.1 |
| MsG0880043891.01.T02 | MTR_0002s1220 | 44.828 | 87 | 47 | 1 | 1 | 86 | 104 | 190 | 1.31e-15 | 70.1 |
| MsG0880043891.01.T02 | MTR_0093s0070 | 44.156 | 77 | 42 | 1 | 1 | 76 | 129 | 205 | 1.97e-15 | 69.7 |
| MsG0880043891.01.T02 | MTR_5g091440 | 39.560 | 91 | 51 | 2 | 1 | 90 | 1 | 88 | 2.10e-15 | 67.8 |
| MsG0880043891.01.T02 | MTR_0015s0120 | 54.839 | 62 | 27 | 1 | 1 | 61 | 299 | 360 | 2.70e-15 | 71.2 |
| MsG0880043891.01.T02 | MTR_1g045340 | 43.678 | 87 | 48 | 1 | 1 | 86 | 90 | 176 | 3.52e-15 | 68.6 |
| MsG0880043891.01.T02 | MTR_5g034450 | 53.448 | 58 | 26 | 1 | 1 | 57 | 55 | 112 | 4.05e-15 | 66.6 |
| MsG0880043891.01.T02 | MTR_3g111390 | 43.678 | 87 | 48 | 1 | 1 | 86 | 104 | 190 | 4.13e-15 | 68.6 |
| MsG0880043891.01.T02 | MTR_1g070820 | 43.678 | 87 | 48 | 1 | 1 | 86 | 104 | 190 | 4.13e-15 | 68.6 |
| MsG0880043891.01.T02 | MTR_1g088650 | 39.796 | 98 | 58 | 1 | 1 | 97 | 92 | 189 | 5.61e-15 | 68.2 |
| MsG0880043891.01.T02 | MTR_5g069700 | 50.746 | 67 | 32 | 1 | 16 | 81 | 2 | 68 | 6.55e-15 | 65.1 |
| MsG0880043891.01.T02 | MTR_7g053400 | 37.037 | 108 | 33 | 2 | 1 | 107 | 181 | 254 | 7.94e-15 | 68.9 |
| MsG0880043891.01.T02 | MTR_4g008270 | 41.026 | 78 | 45 | 1 | 1 | 77 | 16 | 93 | 8.52e-15 | 65.5 |
| MsG0880043891.01.T02 | MTR_1g074090 | 51.667 | 60 | 28 | 1 | 1 | 59 | 87 | 146 | 9.75e-15 | 66.6 |
| MsG0880043891.01.T02 | MTR_1g045560 | 35.185 | 108 | 53 | 2 | 1 | 107 | 1 | 92 | 1.49e-14 | 64.7 |
| MsG0880043891.01.T02 | MTR_5g037920 | 33.333 | 108 | 71 | 1 | 1 | 107 | 104 | 211 | 1.52e-14 | 67.8 |
| MsG0880043891.01.T02 | MTR_5g049680 | 45.588 | 68 | 36 | 1 | 1 | 67 | 37 | 104 | 3.35e-14 | 64.3 |
| MsG0880043891.01.T02 | MTR_1g017840 | 40.506 | 79 | 46 | 1 | 1 | 78 | 84 | 162 | 3.42e-14 | 65.5 |
| MsG0880043891.01.T02 | MTR_8g099580 | 44.872 | 78 | 42 | 1 | 1 | 77 | 212 | 289 | 4.33e-14 | 67.4 |
| MsG0880043891.01.T02 | MTR_8g059135 | 44.595 | 74 | 40 | 1 | 1 | 73 | 34 | 107 | 1.20e-13 | 63.2 |
| MsG0880043891.01.T02 | MTR_8g036780 | 46.875 | 64 | 33 | 1 | 1 | 63 | 40 | 103 | 1.44e-13 | 62.8 |
| MsG0880043891.01.T02 | MTR_1g062840 | 41.892 | 74 | 42 | 1 | 17 | 89 | 2 | 75 | 3.72e-13 | 61.2 |
| MsG0880043891.01.T02 | MTR_2g047100 | 40.426 | 94 | 38 | 3 | 1 | 92 | 24 | 101 | 5.78e-13 | 60.8 |
| MsG0880043891.01.T02 | MTR_7g006380 | 33.333 | 108 | 44 | 2 | 1 | 107 | 72 | 152 | 7.36e-13 | 62.4 |
| MsG0880043891.01.T02 | MTR_3g090920 | 34.146 | 123 | 55 | 5 | 10 | 125 | 2 | 105 | 7.42e-13 | 60.8 |
| MsG0880043891.01.T02 | MTR_4g073820 | 54.717 | 53 | 23 | 1 | 1 | 53 | 1 | 52 | 8.62e-13 | 59.7 |
| MsG0880043891.01.T02 | MTR_6g013970 | 42.466 | 73 | 41 | 1 | 3 | 74 | 107 | 179 | 9.52e-13 | 62.8 |
| MsG0880043891.01.T02 | MTR_8g022750 | 45.070 | 71 | 39 | 0 | 21 | 91 | 215 | 285 | 1.22e-12 | 63.2 |
| MsG0880043891.01.T02 | MTR_1g034430 | 38.462 | 78 | 47 | 1 | 1 | 77 | 101 | 178 | 2.55e-12 | 61.2 |
| MsG0880043891.01.T02 | MTR_7g013520 | 40.541 | 74 | 43 | 1 | 1 | 73 | 76 | 149 | 2.95e-12 | 60.8 |
| MsG0880043891.01.T02 | MTR_4g029360 | 37.634 | 93 | 57 | 1 | 3 | 94 | 135 | 227 | 4.04e-12 | 61.2 |
| MsG0880043891.01.T02 | MTR_1g074240 | 41.538 | 65 | 37 | 1 | 1 | 64 | 6 | 70 | 4.19e-12 | 58.2 |
| MsG0880043891.01.T02 | MTR_5g031620 | 54.386 | 57 | 26 | 0 | 49 | 105 | 139 | 195 | 1.14e-11 | 60.1 |
| MsG0880043891.01.T02 | MTR_8g028785 | 42.308 | 78 | 45 | 0 | 24 | 101 | 288 | 365 | 1.30e-11 | 60.8 |
| MsG0880043891.01.T02 | MTR_8g038340 | 51.923 | 52 | 24 | 1 | 1 | 51 | 195 | 246 | 3.59e-11 | 58.9 |
| MsG0880043891.01.T02 | MTR_6g033225 | 38.095 | 63 | 38 | 1 | 1 | 62 | 76 | 138 | 9.86e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCCAATATCACTTAAAA+TGG | 0.099176 | 8:+32244677 | None:intergenic |
| TCGGGAGAGGTATGTGTATT+AGG | 0.207295 | 8:-32244746 | None:intergenic |
| ATATATATCTATCTCATTAA+TGG | 0.251277 | 8:+32244357 | None:intergenic |
| ATTCATATTTGCGATTACTA+TGG | 0.326355 | 8:+32244559 | None:intergenic |
| AGTGATATTGGAGAGGCTTT+AGG | 0.346687 | 8:-32244669 | MsG0880043891.01.T02:CDS |
| CGCAAATATGAATCGGAACT+TGG | 0.349572 | 8:-32244549 | MsG0880043891.01.T02:CDS |
| GTAATAAGTTCAATACAATT+AGG | 0.386901 | 8:+32244379 | None:intergenic |
| CGGGAGAGGTATGTGTATTA+GGG | 0.403855 | 8:-32244745 | None:intergenic |
| GCAAACTGTTTGCGATAAGA+TGG | 0.461836 | 8:-32244647 | MsG0880043891.01.T02:CDS |
| TCTCCAATATCACTTAAAAT+GGG | 0.485654 | 8:+32244678 | None:intergenic |
| TAGTAATCGCAAATATGAAT+CGG | 0.486193 | 8:-32244556 | MsG0880043891.01.T02:CDS |
| ATGTGTATTAGGGATGATCA+AGG | 0.497045 | 8:-32244735 | MsG0880043891.01.T02:CDS |
| TCGTCTTGACTAAAACTGAA+TGG | 0.509959 | 8:-32244707 | MsG0880043891.01.T02:CDS |
| TAGGCATCTTAGTAAACAAA+CGG | 0.540031 | 8:+32244398 | None:intergenic |
| TATTACGAGACAAGAAAATG+AGG | 0.548704 | 8:-32244463 | MsG0880043891.01.T02:CDS |
| AGGCTTCTCATAGCCTTGCT+AGG | 0.552445 | 8:-32244443 | MsG0880043891.01.T02:CDS |
| GTTTGCGATAAGATGGCTCA+AGG | 0.590470 | 8:-32244640 | MsG0880043891.01.T02:CDS |
| AACAATGTGACTTCCTAGCA+AGG | 0.612744 | 8:+32244430 | None:intergenic |
| TGTGTATTAGGGATGATCAA+GGG | 0.637100 | 8:-32244734 | MsG0880043891.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAATAAGTTCAATACAATT+AGG | + | Chr8:32244724-32244743 | None:intergenic | 20.0% |
| ! | ATTCATATTTGCGATTACTA+TGG | + | Chr8:32244544-32244563 | None:intergenic | 25.0% |
| ! | TAGTAATCGCAAATATGAAT+CGG | - | Chr8:32244544-32244563 | MsG0880043891.01.T02:CDS | 25.0% |
| ! | TCTCCAATATCACTTAAAAT+GGG | + | Chr8:32244425-32244444 | None:intergenic | 25.0% |
| CTCTCCAATATCACTTAAAA+TGG | + | Chr8:32244426-32244445 | None:intergenic | 30.0% | |
| TAGGCATCTTAGTAAACAAA+CGG | + | Chr8:32244705-32244724 | None:intergenic | 30.0% | |
| TATTACGAGACAAGAAAATG+AGG | - | Chr8:32244637-32244656 | MsG0880043891.01.T02:CDS | 30.0% | |
| !! | CATTTTAAGTGATATTGGAG+AGG | - | Chr8:32244424-32244443 | MsG0880043891.01.T02:CDS | 30.0% |
| !! | TCACCCATTTTAAGTGATAT+TGG | - | Chr8:32244419-32244438 | MsG0880043891.01.T02:CDS | 30.0% |
| ATGTGTATTAGGGATGATCA+AGG | - | Chr8:32244365-32244384 | MsG0880043891.01.T02:CDS | 35.0% | |
| TCGTCTTGACTAAAACTGAA+TGG | - | Chr8:32244393-32244412 | MsG0880043891.01.T02:CDS | 35.0% | |
| TGTGTATTAGGGATGATCAA+GGG | - | Chr8:32244366-32244385 | MsG0880043891.01.T02:CDS | 35.0% | |
| AACAATGTGACTTCCTAGCA+AGG | + | Chr8:32244673-32244692 | None:intergenic | 40.0% | |
| AGTGATATTGGAGAGGCTTT+AGG | - | Chr8:32244431-32244450 | MsG0880043891.01.T02:CDS | 40.0% | |
| CGCAAATATGAATCGGAACT+TGG | - | Chr8:32244551-32244570 | MsG0880043891.01.T02:CDS | 40.0% | |
| GCAAACTGTTTGCGATAAGA+TGG | - | Chr8:32244453-32244472 | MsG0880043891.01.T02:CDS | 40.0% | |
| GTTTGCGATAAGATGGCTCA+AGG | - | Chr8:32244460-32244479 | MsG0880043891.01.T02:CDS | 45.0% | |
| ! | AGGCTTCTCATAGCCTTGCT+AGG | - | Chr8:32244657-32244676 | MsG0880043891.01.T02:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 32244365 | 32244757 | 32244365 | ID=MsG0880043891.01;Name=MsG0880043891.01 |
| Chr8 | mRNA | 32244365 | 32244757 | 32244365 | ID=MsG0880043891.01.T01;Parent=MsG0880043891.01;Name=MsG0880043891.01.T01;_AED=0.29;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|103 |
| Chr8 | exon | 32244365 | 32244650 | 32244365 | ID=MsG0880043891.01.T01:exon:7857;Parent=MsG0880043891.01.T01 |
| Chr8 | exon | 32244732 | 32244757 | 32244732 | ID=MsG0880043891.01.T01:exon:7856;Parent=MsG0880043891.01.T01 |
| Chr8 | CDS | 32244732 | 32244757 | 32244732 | ID=MsG0880043891.01.T01:cds;Parent=MsG0880043891.01.T01 |
| Chr8 | CDS | 32244365 | 32244650 | 32244365 | ID=MsG0880043891.01.T01:cds;Parent=MsG0880043891.01.T01 |
| Chr8 | mRNA | 32244365 | 32244757 | 32244365 | ID=MsG0880043891.01.T02;Parent=MsG0880043891.01;Name=MsG0880043891.01.T02;_AED=0.23;_eAED=0.36;_QI=0|-1|0|1|-1|0|1|0|130 |
| Chr8 | exon | 32244365 | 32244757 | 32244365 | ID=MsG0880043891.01.T02:exon:7858;Parent=MsG0880043891.01.T02 |
| Chr8 | CDS | 32244365 | 32244757 | 32244365 | ID=MsG0880043891.01.T02:cds;Parent=MsG0880043891.01.T02 |
| Gene Sequence |
| Protein sequence |
