AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043893.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043893.01.T01 MTR_5g036320 81.356 59 11 0 1 59 85 143 5.66e-29 101
MsG0880043893.01.T01 MTR_7g063280 46.591 88 47 0 1 88 78 165 8.56e-29 101
MsG0880043893.01.T01 MTR_2g059470 55.128 78 35 0 1 78 6 83 1.61e-26 93.2
MsG0880043893.01.T01 MTR_5g075610 54.545 88 38 1 1 88 1 86 6.11e-26 92.4
MsG0880043893.01.T01 MTR_6g016265 54.321 81 37 0 1 81 45 125 5.63e-25 90.5
MsG0880043893.01.T01 MTR_8g088220 52.703 74 35 0 5 78 4 77 1.39e-23 85.9
MsG0880043893.01.T01 MTR_3g116210 44.186 86 48 0 1 86 103 188 1.84e-23 88.6
MsG0880043893.01.T01 MTR_6g453270 52.055 73 35 0 1 73 306 378 5.03e-23 90.9
MsG0880043893.01.T01 MTR_1g081930 49.398 83 42 0 1 83 44 126 8.03e-23 85.5
MsG0880043893.01.T01 MTR_8g098925 45.455 88 48 0 1 88 71 158 2.15e-22 85.1
MsG0880043893.01.T01 MTR_1g069475 50.685 73 36 0 2 74 171 243 4.12e-22 86.3
MsG0880043893.01.T01 MTR_5g083200 48.864 88 45 0 1 88 104 191 4.95e-22 85.1
MsG0880043893.01.T01 MTR_0002s1220 48.864 88 45 0 1 88 104 191 4.95e-22 85.1
MsG0880043893.01.T01 MTR_1g045340 47.727 88 46 0 1 88 90 177 1.51e-21 83.6
MsG0880043893.01.T01 MTR_3g111390 47.727 88 46 0 1 88 104 191 1.94e-21 83.6
MsG0880043893.01.T01 MTR_1g070820 47.727 88 46 0 1 88 104 191 1.94e-21 83.6
MsG0880043893.01.T01 MTR_0093s0070 49.351 77 39 0 1 77 129 205 3.65e-21 83.2
MsG0880043893.01.T01 MTR_6g089560 45.783 83 45 0 1 83 122 204 1.26e-20 82.0
MsG0880043893.01.T01 MTR_5g085210 48.193 83 43 0 1 83 125 207 1.55e-20 82.0
MsG0880043893.01.T01 MTR_4g008270 47.436 78 41 0 1 78 16 93 1.56e-20 78.6
MsG0880043893.01.T01 MTR_0015s0120 57.576 66 28 0 1 66 299 364 1.73e-20 83.6
MsG0880043893.01.T01 MTR_8g059135 47.727 88 46 0 1 88 34 121 2.15e-20 79.0
MsG0880043893.01.T01 MTR_5g049680 50.704 71 34 1 1 70 37 107 4.08e-20 77.8
MsG0880043893.01.T01 MTR_2g027020 47.126 87 42 1 2 88 35 117 6.44e-20 79.3
MsG0880043893.01.T01 MTR_1g017840 46.154 78 42 0 1 78 84 161 8.05e-20 78.6
MsG0880043893.01.T01 MTR_5g069700 53.731 67 31 0 16 82 2 68 2.31e-19 75.1
MsG0880043893.01.T01 MTR_1g050488 53.333 60 28 0 2 61 105 164 3.14e-19 77.0
MsG0880043893.01.T01 MTR_5g034450 56.897 58 25 0 1 58 55 112 3.92e-19 75.5
MsG0880043893.01.T01 MTR_1g034430 44.304 79 44 0 1 79 101 179 4.42e-19 77.0
MsG0880043893.01.T01 MTR_1g088650 45.455 88 48 0 1 88 92 179 4.53e-19 77.4
MsG0880043893.01.T01 MTR_1g074090 53.333 60 28 0 1 60 87 146 2.12e-18 74.7
MsG0880043893.01.T01 MTR_6g034915 49.275 69 35 0 1 69 61 129 2.15e-18 75.1
MsG0880043893.01.T01 MTR_8g099580 50.000 78 39 0 1 78 212 289 2.94e-18 77.0
MsG0880043893.01.T01 MTR_5g091440 44.048 84 44 1 1 84 1 81 8.06e-18 72.4
MsG0880043893.01.T01 MTR_1g062840 44.444 72 40 0 17 88 2 73 1.18e-17 70.9
MsG0880043893.01.T01 MTR_1g045560 52.381 63 30 0 1 63 1 63 1.22e-17 71.2
MsG0880043893.01.T01 MTR_7g013520 47.297 74 39 0 1 74 76 149 3.00e-17 72.4
MsG0880043893.01.T01 MTR_8g031800 45.714 70 38 0 1 70 37 106 5.00e-17 70.1
MsG0880043893.01.T01 MTR_4g029360 43.023 86 49 0 3 88 135 220 6.65e-17 72.4
MsG0880043893.01.T01 MTR_1g074240 47.692 65 34 0 1 65 6 70 9.90e-17 68.2
MsG0880043893.01.T01 MTR_7g053400 55.357 56 25 0 1 56 181 236 2.17e-16 71.2
MsG0880043893.01.T01 MTR_8g036780 51.667 60 29 0 1 60 40 99 4.22e-16 67.8
MsG0880043893.01.T01 MTR_8g079540 48.529 68 35 0 1 68 54 121 5.93e-16 67.4
MsG0880043893.01.T01 MTR_6g016135 51.724 58 28 0 1 58 385 442 2.28e-15 69.7
MsG0880043893.01.T01 MTR_6g013970 43.836 73 41 0 3 75 107 179 2.84e-15 67.8
MsG0880043893.01.T01 MTR_5g037920 38.961 77 46 1 1 77 104 179 4.61e-15 67.4
MsG0880043893.01.T01 MTR_8g038340 55.769 52 23 0 1 52 195 246 1.06e-14 66.6
MsG0880043893.01.T01 MTR_7g072030 50.000 52 26 0 15 66 69 120 1.16e-14 64.3
MsG0880043893.01.T01 MTR_2g047100 42.045 88 36 1 1 88 24 96 1.44e-14 63.5
MsG0880043893.01.T01 MTR_4g073820 55.556 54 22 1 1 54 1 52 1.81e-14 62.4
MsG0880043893.01.T01 MTR_3g465840 45.588 68 37 0 21 88 1 68 2.31e-14 64.3
MsG0880043893.01.T01 MTR_3g026270 47.273 55 29 0 1 55 1 55 2.67e-14 62.0
MsG0880043893.01.T01 MTR_8g022750 48.529 68 35 0 21 88 214 281 3.23e-14 65.9
MsG0880043893.01.T01 MTR_6g033225 38.095 63 39 0 1 63 76 138 3.46e-14 63.5
MsG0880043893.01.T01 MTR_4g006490 54.000 50 23 0 1 50 34 83 4.23e-13 59.3
MsG0880043893.01.T01 MTR_6g045427 50.847 59 29 0 27 85 177 235 7.59e-13 61.6
MsG0880043893.01.T01 MTR_8g028785 49.231 65 33 0 24 88 287 351 1.00e-12 62.0
MsG0880043893.01.T01 MTR_1g017820 43.860 57 32 0 8 64 13 69 1.18e-12 57.8
MsG0880043893.01.T01 MTR_1g109000 51.923 52 25 0 3 54 34 85 3.06e-12 57.0
MsG0880043893.01.T01 MTR_1g022215 47.059 51 27 0 1 51 20 70 1.67e-11 55.5
MsG0880043893.01.T01 MTR_2g055490 48.276 58 30 0 1 58 13 70 2.55e-11 55.5
MsG0880043893.01.T01 MTR_6g016355 42.593 54 31 0 3 56 124 177 3.21e-11 56.6
MsG0880043893.01.T01 MTR_4g094272 58.537 41 17 0 2 42 100 140 3.93e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGGATAGGTATGTGTATT+AGG 0.156948 8:-32294508 None:intergenic
TTGTCTTGGCTAAAACTAAA+TGG 0.222579 8:-32294469 MsG0880043893.01.T01:CDS
CCCATTCTTAAAGTTGATAT+TGG 0.222677 8:-32294440 MsG0880043893.01.T01:CDS
ATTCATCTTTGCGACTACTA+TGG 0.307613 8:+32294318 None:intergenic
GTTGATATTGGAGAGGCTTT+AGG 0.347941 8:-32294428 MsG0880043893.01.T01:CDS
TCCAATATCAACTTTAAGAA+TGG 0.370767 8:+32294439 None:intergenic
CGCAAAGATGAATCGGAACT+TGG 0.409159 8:-32294308 MsG0880043893.01.T01:CDS
GGCTTTAGGCCTACTGTTTG+CGG 0.426815 8:-32294414 MsG0880043893.01.T01:CDS
CGGGATAGGTATGTGTATTA+GGG 0.432873 8:-32294507 None:intergenic
CCAATATCAACTTTAAGAAT+GGG 0.453486 8:+32294440 None:intergenic
GCCTACTGTTTGCGGTAAGA+TGG 0.465474 8:-32294406 MsG0880043893.01.T01:CDS
CGATCAAGGGAGCTTTGTCT+TGG 0.492287 8:-32294483 MsG0880043893.01.T01:CDS
TAGTAGTCGCAAAGATGAAT+CGG 0.504796 8:-32294315 MsG0880043893.01.T01:CDS
ATGTGTATTAGGGACGATCA+AGG 0.539176 8:-32294497 MsG0880043893.01.T01:CDS
TCTTAAAGTTGATATTGGAG+AGG 0.619119 8:-32294435 MsG0880043893.01.T01:CDS
TGTGTATTAGGGACGATCAA+GGG 0.655277 8:-32294496 MsG0880043893.01.T01:CDS
GCCATCTTACCGCAAACAGT+AGG 0.679350 8:+32294405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAATATCAACTTTAAGAAT+GGG + Chr8:32294313-32294332 None:intergenic 25.0%
!! TCCAATATCAACTTTAAGAA+TGG + Chr8:32294314-32294333 None:intergenic 25.0%
TTGTCTTGGCTAAAACTAAA+TGG - Chr8:32294281-32294300 MsG0880043893.01.T01:CDS 30.0%
!! CCCATTCTTAAAGTTGATAT+TGG - Chr8:32294310-32294329 MsG0880043893.01.T01:CDS 30.0%
!! TCTTAAAGTTGATATTGGAG+AGG - Chr8:32294315-32294334 MsG0880043893.01.T01:CDS 30.0%
ATTCATCTTTGCGACTACTA+TGG + Chr8:32294435-32294454 None:intergenic 35.0%
TAGTAGTCGCAAAGATGAAT+CGG - Chr8:32294435-32294454 MsG0880043893.01.T01:CDS 35.0%
ATGTGTATTAGGGACGATCA+AGG - Chr8:32294253-32294272 MsG0880043893.01.T01:CDS 40.0%
GTTGATATTGGAGAGGCTTT+AGG - Chr8:32294322-32294341 MsG0880043893.01.T01:CDS 40.0%
TGTGTATTAGGGACGATCAA+GGG - Chr8:32294254-32294273 MsG0880043893.01.T01:CDS 40.0%
CGCAAAGATGAATCGGAACT+TGG - Chr8:32294442-32294461 MsG0880043893.01.T01:CDS 45.0%
GCCATCTTACCGCAAACAGT+AGG + Chr8:32294348-32294367 None:intergenic 50.0%
GCCTACTGTTTGCGGTAAGA+TGG - Chr8:32294344-32294363 MsG0880043893.01.T01:CDS 50.0%
! CGATCAAGGGAGCTTTGTCT+TGG - Chr8:32294267-32294286 MsG0880043893.01.T01:CDS 50.0%
!! GGCTTTAGGCCTACTGTTTG+CGG - Chr8:32294336-32294355 MsG0880043893.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 32294253 32294519 32294253 ID=MsG0880043893.01;Name=MsG0880043893.01
Chr8 mRNA 32294253 32294519 32294253 ID=MsG0880043893.01.T01;Parent=MsG0880043893.01;Name=MsG0880043893.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|88
Chr8 exon 32294253 32294519 32294253 ID=MsG0880043893.01.T01:exon:7833;Parent=MsG0880043893.01.T01
Chr8 CDS 32294253 32294519 32294253 ID=MsG0880043893.01.T01:cds;Parent=MsG0880043893.01.T01
Gene Sequence

>MsG0880043893.01.T01

ATGTGTATTAGGGACGATCAAGGGAGCTTTGTCTTGGCTAAAACTAAATGGATATCACCCATTCTTAAAGTTGATATTGGAGAGGCTTTAGGCCTACTGTTTGCGGTAAGATGGCTCAAAGATTTGCATATTAATAATGTGATTTTTGAGTTAGACTCTAAGAGAGTTGTTGATAGCTTCCATAGTAGTCGCAAAGATGAATCGGAACTTGGAGCTATTATTAGAGAGTGTAGAAATTCATTCTCATCTTTTTTCACAAACTCATAG
Protein sequence

>MsG0880043893.01.T01

MCIRDDQGSFVLAKTKWISPILKVDIGEALGLLFAVRWLKDLHINNVIFELDSKRVVDSFHSSRKDESELGAIIRECRNSFSSFFTNS*