AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043950.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCAAGGATGACCTTTA+AGG 0.194450 8:+33253918 None:intergenic
AAGGGCTACATGGAACATTT+TGG 0.246156 8:-33253819 MsG0880043950.01.T01:CDS
CATCCTTGAAGTAATTTATA+AGG 0.251367 8:-33253908 MsG0880043950.01.T01:CDS
GAGCATTATAAGGTCCTTAA+AGG 0.313319 8:-33253932 MsG0880043950.01.T01:CDS
GAAGTAATTTATAAGGTTGA+TGG 0.349080 8:-33253901 MsG0880043950.01.T01:CDS
CAACCTTATAAATTACTTCA+AGG 0.393345 8:+33253905 None:intergenic
AGATATTGATGAGCATTATA+AGG 0.502676 8:-33253942 MsG0880043950.01.T01:CDS
GATGGCACTGCTGCTGCTAA+AGG 0.508067 8:-33253883 MsG0880043950.01.T01:CDS
TTGACCCTCCAAGGGCTACA+TGG 0.511243 8:-33253829 MsG0880043950.01.T01:CDS
ATGAGGATATTGACCCTCCA+AGG 0.524252 8:-33253838 MsG0880043950.01.T01:CDS
AAACACTTATAAGCCTTTGG+TGG 0.528376 8:-33253965 MsG0880043950.01.T01:CDS
ATGTTCCATGTAGCCCTTGG+AGG 0.532067 8:+33253824 None:intergenic
AAAATGTTCCATGTAGCCCT+TGG 0.537621 8:+33253821 None:intergenic
CTCATCAATATCTCCACCAA+AGG 0.579449 8:+33253952 None:intergenic
TGTTCCATGTAGCCCTTGGA+GGG 0.608171 8:+33253825 None:intergenic
TGAGGATATTGACCCTCCAA+GGG 0.640440 8:-33253837 MsG0880043950.01.T01:CDS
TTAATATGAGAAGATCAATG+AGG 0.727164 8:-33253855 MsG0880043950.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAAACACTTATAAGCCTT+TGG - Chr8:33253842-33253861 MsG0880043950.01.T01:CDS 25.0%
! AGATATTGATGAGCATTATA+AGG - Chr8:33253868-33253887 MsG0880043950.01.T01:CDS 25.0%
! CAACCTTATAAATTACTTCA+AGG + Chr8:33253908-33253927 None:intergenic 25.0%
! CATCCTTGAAGTAATTTATA+AGG - Chr8:33253902-33253921 MsG0880043950.01.T01:CDS 25.0%
! GAAGTAATTTATAAGGTTGA+TGG - Chr8:33253909-33253928 MsG0880043950.01.T01:CDS 25.0%
! TTAATATGAGAAGATCAATG+AGG - Chr8:33253955-33253974 MsG0880043950.01.T01:CDS 25.0%
AAACACTTATAAGCCTTTGG+TGG - Chr8:33253845-33253864 MsG0880043950.01.T01:CDS 35.0%
GAGCATTATAAGGTCCTTAA+AGG - Chr8:33253878-33253897 MsG0880043950.01.T01:CDS 35.0%
TACTTCAAGGATGACCTTTA+AGG + Chr8:33253895-33253914 None:intergenic 35.0%
AAAATGTTCCATGTAGCCCT+TGG + Chr8:33253992-33254011 None:intergenic 40.0%
CTCATCAATATCTCCACCAA+AGG + Chr8:33253861-33253880 None:intergenic 40.0%
! AAGGGCTACATGGAACATTT+TGG - Chr8:33253991-33254010 MsG0880043950.01.T01:CDS 40.0%
ATGAGGATATTGACCCTCCA+AGG - Chr8:33253972-33253991 MsG0880043950.01.T01:CDS 45.0%
TGAGGATATTGACCCTCCAA+GGG - Chr8:33253973-33253992 MsG0880043950.01.T01:CDS 45.0%
ATGTTCCATGTAGCCCTTGG+AGG + Chr8:33253989-33254008 None:intergenic 50.0%
TGTTCCATGTAGCCCTTGGA+GGG + Chr8:33253988-33254007 None:intergenic 50.0%
GATGGCACTGCTGCTGCTAA+AGG - Chr8:33253927-33253946 MsG0880043950.01.T01:CDS 55.0%
TTGACCCTCCAAGGGCTACA+TGG - Chr8:33253981-33254000 MsG0880043950.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33253807 33254025 33253807 ID=MsG0880043950.01;Name=MsG0880043950.01
Chr8 mRNA 33253807 33254025 33253807 ID=MsG0880043950.01.T01;Parent=MsG0880043950.01;Name=MsG0880043950.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72
Chr8 exon 33253807 33254025 33253807 ID=MsG0880043950.01.T01:exon:10160;Parent=MsG0880043950.01.T01
Chr8 CDS 33253807 33254025 33253807 ID=MsG0880043950.01.T01:cds;Parent=MsG0880043950.01.T01
Gene Sequence

>MsG0880043950.01.T01

ATGAAGAGTGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCCTTTGGTGGAGATATTGATGAGCATTATAAGGTCCTTAAAGGTCATCCTTGAAGTAATTTATAAGGTTGATGGCACTGCTGCTGCTAAAGGACAGTTTAATATGAGAAGATCAATGAGGATATTGACCCTCCAAGGGCTACATGGAACATTTTGGCAAAGTCACTAG
Protein sequence

>MsG0880043950.01.T01

MKSVEEVDELNKKNTYKPLVEILMSIIRSLKVILEVIYKVDGTAAAKGQFNMRRSMRILTLQGLHGTFWQSH*