AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043956.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043956.01.T01 MTR_8g045570 94.624 93 5 0 33 125 60 152 1.12e-60 183
MsG0880043956.01.T01 MTR_8g045490 76.344 93 22 0 33 125 60 152 9.78e-49 153
MsG0880043956.01.T01 MTR_8g045520 72.826 92 25 0 33 124 60 151 1.27e-45 145
MsG0880043956.01.T01 MTR_8g045400 70.968 93 26 1 33 125 60 151 3.34e-44 142
MsG0880043956.01.T01 MTR_8g045555 69.892 93 28 0 33 125 60 152 9.21e-44 140
MsG0880043956.01.T01 MTR_8g045695 68.817 93 28 1 33 125 60 151 4.91e-42 136
MsG0880043956.01.T01 MTR_8g045735 67.742 93 29 1 33 125 60 151 6.22e-42 136
MsG0880043956.01.T01 MTR_8g045300 65.217 92 32 0 33 124 60 151 1.41e-41 135
MsG0880043956.01.T01 MTR_8g045665 66.667 93 30 1 33 125 60 151 2.44e-40 132
MsG0880043956.01.T01 MTR_8g045560 69.663 89 26 1 37 125 64 151 7.20e-40 130
MsG0880043956.01.T01 MTR_8g045640 69.663 89 26 1 37 125 64 151 1.94e-39 129
MsG0880043956.01.T01 MTR_1g030810 44.211 95 51 1 32 124 58 152 1.65e-20 81.6
MsG0880043956.01.T01 MTR_1g030820 41.053 95 54 1 32 124 58 152 3.12e-18 75.5
MsG0880043956.01.T01 MTR_8g012550 41.111 90 52 1 33 122 62 150 3.13e-18 75.9
MsG0880043956.01.T01 MTR_1g030840 45.783 83 39 2 46 122 73 155 6.38e-15 67.4
MsG0880043956.01.T01 MTR_3g013550 70.732 41 9 1 1 41 1 38 4.29e-14 64.7
MsG0880043956.01.T01 MTR_4g094140 72.973 37 10 0 1 37 1 37 1.28e-13 61.6
MsG0880043956.01.T01 MTR_3g060310 70.270 37 11 0 1 37 1 37 7.22e-13 59.7
MsG0880043956.01.T01 MTR_1g031640 38.298 94 52 2 32 125 60 147 3.15e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043956.01.T01 AT5G28010 38.043 92 56 1 33 123 72 163 1.00e-16 72.0
MsG0880043956.01.T01 AT5G28010 38.043 92 56 1 33 123 72 163 1.00e-16 72.0
MsG0880043956.01.T01 AT1G70870 39.326 89 53 1 37 125 52 139 2.36e-16 70.5
MsG0880043956.01.T01 AT1G23120 37.363 91 56 1 33 123 56 145 4.20e-16 70.1
MsG0880043956.01.T01 AT1G23120 37.363 91 56 1 33 123 106 195 1.01e-15 70.1
MsG0880043956.01.T01 AT1G70850 35.870 92 58 1 33 123 222 313 1.96e-13 65.5
MsG0880043956.01.T01 AT1G70850 31.522 92 62 1 33 123 66 157 3.38e-11 59.3
MsG0880043956.01.T01 AT1G70850 35.870 92 58 1 33 123 222 313 1.96e-13 65.5
MsG0880043956.01.T01 AT1G70850 31.522 92 62 1 33 123 66 157 3.38e-11 59.3
MsG0880043956.01.T01 AT1G24020 38.372 86 52 1 39 124 67 151 4.28e-13 62.4
MsG0880043956.01.T01 AT1G24020 38.372 86 52 1 39 124 67 151 4.28e-13 62.4
MsG0880043956.01.T01 AT1G70840 32.609 92 61 1 33 123 77 168 4.32e-13 62.8
MsG0880043956.01.T01 AT1G70880 32.609 92 61 1 33 123 65 156 1.09e-12 61.6
MsG0880043956.01.T01 AT1G70830 31.579 95 64 1 30 123 29 123 1.26e-12 63.2
MsG0880043956.01.T01 AT5G28000 34.783 92 59 1 33 123 70 161 1.98e-12 60.8
MsG0880043956.01.T01 AT1G70890 33.696 92 60 1 33 123 64 155 2.24e-12 60.5
MsG0880043956.01.T01 AT1G70830 31.522 92 62 1 33 123 79 170 6.55e-12 59.7
MsG0880043956.01.T01 AT1G70830 31.522 92 62 1 33 123 79 170 8.83e-12 59.7
MsG0880043956.01.T01 AT1G70830 31.522 92 62 1 33 123 79 170 2.14e-11 59.3
MsG0880043956.01.T01 AT4G23670 38.462 78 48 0 48 125 74 151 2.15e-11 57.8
MsG0880043956.01.T01 AT1G70830 31.522 92 62 1 33 123 79 170 2.16e-11 59.7
MsG0880043956.01.T01 AT1G70850 31.522 92 62 1 33 123 66 157 2.52e-11 58.9

Find 30 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAGTGCTGCTGTTAAA+TGG 0.274336 8:-33380938 MsG0880043956.01.T01:CDS
ATCCATGTCTCTAGTGCTTT+TGG 0.286969 8:+33380851 None:intergenic
TGATCCTCCAAATGGCTATA+TGG 0.321241 8:-33380883 MsG0880043956.01.T01:CDS
GGCCGGTTCCTATGGAATTC+GGG 0.332114 8:-33385881 MsG0880043956.01.T01:CDS
AGGCCGGTTCCTATGGAATT+CGG 0.339841 8:-33385882 MsG0880043956.01.T01:CDS
CCTTAGTTCCACATTCGTAT+AGG 0.405990 8:+33385902 None:intergenic
AAAATCACTTTCAAGCTCTT+CGG 0.431537 8:-33381023 MsG0880043956.01.T01:CDS
CGGCCTTATCAATTACTTCA+AGG 0.435763 8:+33380961 None:intergenic
GCCCCGAATTCCATAGGAAC+CGG 0.454272 8:+33385879 None:intergenic
GGAACTAAGGCCGGTTCCTA+TGG 0.462209 8:-33385889 MsG0880043956.01.T01:CDS
CATCCTTGAAGTAATTGATA+AGG 0.489945 8:-33380964 MsG0880043956.01.T01:CDS
AAGTAGTCCATATAGCCATT+TGG 0.505544 8:+33380876 None:intergenic
GAGGATATTGATCCTCCAAA+TGG 0.518489 8:-33380891 MsG0880043956.01.T01:CDS
ATCACTTTCAAGCTCTTCGG+TGG 0.530357 8:-33381020 MsG0880043956.01.T01:CDS
CCTATACGAATGTGGAACTA+AGG 0.532384 8:-33385902 MsG0880043956.01.T01:CDS
TACGAATGTGGAACTAAGGC+CGG 0.535755 8:-33385898 MsG0880043956.01.T01:CDS
GGAGTGGAGTACATAGCTAC+AGG 0.561463 8:-33385944 MsG0880043956.01.T01:CDS
TTTAACAGCAGCACTTCCAC+CGG 0.572085 8:+33380941 None:intergenic
TATTCTGCCCCGAATTCCAT+AGG 0.584216 8:+33385873 None:intergenic
TAGTCCATATAGCCATTTGG+AGG 0.593989 8:+33380879 None:intergenic
CATGGATGCTCATCTTGTCA+AGG 0.600309 8:-33380835 MsG0880043956.01.T01:CDS
CTTGAAGTAATTGATAAGGC+CGG 0.613456 8:-33380960 MsG0880043956.01.T01:CDS
AGACATTGATGAACACTACA+AGG 0.618368 8:-33380997 MsG0880043956.01.T01:CDS
TGCCAAAAGCACTAGAGACA+TGG 0.621841 8:-33380853 MsG0880043956.01.T01:CDS
GATCTATACTCTATGTGGAG+TGG 0.628757 8:-33385960 None:intergenic
GCCGGTTCCTATGGAATTCG+GGG 0.632937 8:-33385880 MsG0880043956.01.T01:CDS
TAAACTCACCTATACGAATG+TGG 0.632971 8:-33385910 MsG0880043956.01.T01:CDS
ACATCACAACACAGATGGCA+AGG 0.635566 8:-33381093 MsG0880043956.01.T01:intron
GAAGTAATTGATAAGGCCGG+TGG 0.666595 8:-33380957 MsG0880043956.01.T01:CDS
TGAATATGAGAAAATCAATG+AGG 0.668209 8:-33380910 MsG0880043956.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAATGAATAGTATT+TGG - Chr8:33385019-33385038 MsG0880043956.01.T01:intron 10.0%
!! TATAAAAATCTATATAATCA+AGG + Chr8:33385407-33385426 None:intergenic 10.0%
!! TATAGAAATATATATAATCA+AGG + Chr8:33385627-33385646 None:intergenic 10.0%
!! TTATAAAAACTTACTATTAA+AGG - Chr8:33385422-33385441 MsG0880043956.01.T01:intron 10.0%
!!! AAAAAAATAAGAGTTTTTTA+AGG - Chr8:33383524-33383543 MsG0880043956.01.T01:intron 10.0%
!!! ATCTTTAATAATCTAAAATT+TGG - Chr8:33385341-33385360 MsG0880043956.01.T01:intron 10.0%
!!! ATTATACTATTCTTTTTTTT+TGG + Chr8:33383474-33383493 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTTTGTTG+AGG + Chr8:33383269-33383288 None:intergenic 10.0%
!!! TAAAAAACTCTTATTTTTTT+TGG + Chr8:33383524-33383543 None:intergenic 10.0%
!! AAAGAAAATAGATAAAAAGT+TGG - Chr8:33383957-33383976 MsG0880043956.01.T01:intron 15.0%
!! AAATATAAATGTATAGTGTT+TGG + Chr8:33385142-33385161 None:intergenic 15.0%
!! AAGAATCTAAGTAATAAATT+TGG + Chr8:33383993-33384012 None:intergenic 15.0%
!! AATATAAATGTATAGTGTTT+GGG + Chr8:33385141-33385160 None:intergenic 15.0%
!! TAAAATAAAACAAGATGATT+TGG + Chr8:33382942-33382961 None:intergenic 15.0%
!! TAGCTATTTAAAAAAAATCA+CGG + Chr8:33383297-33383316 None:intergenic 15.0%
!! TTAAACTAATCTATCAAAAT+TGG - Chr8:33383089-33383108 MsG0880043956.01.T01:intron 15.0%
!! TTACTAACAACAATTAATAA+AGG + Chr8:33384462-33384481 None:intergenic 15.0%
!! TTTATTTATCATGATATTGA+TGG - Chr8:33384627-33384646 MsG0880043956.01.T01:intron 15.0%
!!! AAAATATTTTAGCAAAACTT+GGG + Chr8:33381605-33381624 None:intergenic 15.0%
!!! AAGAAAAAATATTTTTTTGC+TGG + Chr8:33385518-33385537 None:intergenic 15.0%
!!! AATAATGATTTTTTTGGTAT+TGG + Chr8:33381480-33381499 None:intergenic 15.0%
!!! AATTATGTTTTTATCTTTGA+AGG - Chr8:33384118-33384137 MsG0880043956.01.T01:intron 15.0%
!!! CTTTCTAATAATGATTTTTT+TGG + Chr8:33381486-33381505 None:intergenic 15.0%
!!! TAATTTGTAGGTTATTAATT+TGG - Chr8:33382577-33382596 MsG0880043956.01.T01:intron 15.0%
!!! TATTTGTACAAATATCATTT+TGG + Chr8:33383831-33383850 None:intergenic 15.0%
!!! TTATTTTTACGTGAAATAAT+CGG - Chr8:33383608-33383627 MsG0880043956.01.T01:intron 15.0%
!! AACATCTCAAAATATCAATA+TGG + Chr8:33382109-33382128 None:intergenic 20.0%
!! AACTAATTAATGAGAAACTT+TGG + Chr8:33381795-33381814 None:intergenic 20.0%
!! ACTAATTAATGAGAAACTTT+GGG + Chr8:33381794-33381813 None:intergenic 20.0%
!! ATAAAAAACAATAGAGAGAA+AGG + Chr8:33384285-33384304 None:intergenic 20.0%
!! ATTAGTAGTGTTTATATAGT+GGG - Chr8:33381267-33381286 MsG0880043956.01.T01:intron 20.0%
!! TATTATTCTTCTTCAATTCA+TGG - Chr8:33382032-33382051 MsG0880043956.01.T01:intron 20.0%
!! TGAGATGTTAAAAATTAGAA+TGG - Chr8:33382120-33382139 MsG0880043956.01.T01:intron 20.0%
!! TGGTTAAAAATCTGAATAAA+TGG - Chr8:33383187-33383206 MsG0880043956.01.T01:intron 20.0%
!! TGTTAGTAAAGTAGTTAAAT+TGG - Chr8:33384473-33384492 MsG0880043956.01.T01:intron 20.0%
!! TTCAGCAGAAATAAAATTTA+TGG + Chr8:33383332-33383351 None:intergenic 20.0%
!! TTTAAAAGGTTGTATGATTA+GGG - Chr8:33383559-33383578 MsG0880043956.01.T01:intron 20.0%
!!! AAATATTTTAGCAAAACTTG+GGG + Chr8:33381604-33381623 None:intergenic 20.0%
!!! AACCATTTTTTAAATTCAAG+TGG - Chr8:33381123-33381142 MsG0880043956.01.T01:intron 20.0%
!!! AATAATGATTTTTTTGGTAG+TGG + Chr8:33381563-33381582 None:intergenic 20.0%
!!! ACCATTTTTTAAATTCAAGT+GGG - Chr8:33381124-33381143 MsG0880043956.01.T01:intron 20.0%
!!! ACTTGATCTGAAATATATTT+TGG - Chr8:33381696-33381715 MsG0880043956.01.T01:intron 20.0%
!!! ATTGCTTTGTTTAATTAAAC+TGG + Chr8:33383784-33383803 None:intergenic 20.0%
!!! CAAAATATTTTAGCAAAACT+TGG + Chr8:33381606-33381625 None:intergenic 20.0%
!!! CAAGTTTTGCTAAAATATTT+TGG - Chr8:33381604-33381623 MsG0880043956.01.T01:intron 20.0%
!!! CTAATCTTTTTGTTTTGAAT+AGG - Chr8:33383066-33383085 MsG0880043956.01.T01:intron 20.0%
!!! GGTAGTAATATTTAATTTGT+AGG - Chr8:33382565-33382584 MsG0880043956.01.T01:intron 20.0%
!!! TATAAACACAATGTTGTTTT+TGG + Chr8:33382482-33382501 None:intergenic 20.0%
!!! TATTTTTACGTGAAATAATC+GGG - Chr8:33383609-33383628 MsG0880043956.01.T01:intron 20.0%
! AAAACAAAGACACCTATAAA+AGG + Chr8:33385251-33385270 None:intergenic 25.0%
! AAAACCAAAATACAACAGTT+TGG - Chr8:33382452-33382471 MsG0880043956.01.T01:intron 25.0%
! AAACCAAAATACAACAGTTT+GGG - Chr8:33382453-33382472 MsG0880043956.01.T01:intron 25.0%
! ACAATGCATAAAAATATGCA+AGG - Chr8:33383423-33383442 MsG0880043956.01.T01:intron 25.0%
! CATTAGTAGTGTTTATATAG+TGG - Chr8:33381266-33381285 MsG0880043956.01.T01:intron 25.0%
! CATTCTTATTAAACCGAAAA+GGG + Chr8:33381998-33382017 None:intergenic 25.0%
! GATGTTAAAAATTAGAATGG+TGG - Chr8:33382123-33382142 MsG0880043956.01.T01:intron 25.0%
! GATTTAGTCATTTGAGAAAA+AGG + Chr8:33385063-33385082 None:intergenic 25.0%
! GGTGTTCATAAAAGTTTAAA+AGG - Chr8:33383545-33383564 MsG0880043956.01.T01:intron 25.0%
! GTTTAAAAGGTTGTATGATT+AGG - Chr8:33383558-33383577 MsG0880043956.01.T01:intron 25.0%
! TAAACACAAATTCTCATGTT+GGG - Chr8:33381185-33381204 MsG0880043956.01.T01:intron 25.0%
! TAAATATTACTACCACTTGA+TGG + Chr8:33382559-33382578 None:intergenic 25.0%
! TACATGTTAAAGTACAAATG+AGG - Chr8:33384050-33384069 MsG0880043956.01.T01:intron 25.0%
! TATCCACTATGTAAATCTTA+TGG - Chr8:33381075-33381094 MsG0880043956.01.T01:CDS 25.0%
! TATTGTGTTAGTTAGTGTTT+GGG + Chr8:33384671-33384690 None:intergenic 25.0%
! TCATTCTTATTAAACCGAAA+AGG + Chr8:33381999-33382018 None:intergenic 25.0%
! TGAATATGAGAAAATCAATG+AGG - Chr8:33385868-33385887 MsG0880043956.01.T01:CDS 25.0%
! TGAGAATAGTTTAAAGAACT+TGG + Chr8:33382774-33382793 None:intergenic 25.0%
! TGCATAAAAATATCAAACCT+CGG - Chr8:33381454-33381473 MsG0880043956.01.T01:intron 25.0%
! TGGTTAAAAATCTGAAGAAA+TGG - Chr8:33383243-33383262 MsG0880043956.01.T01:intron 25.0%
! TGTAAGCAAATCTCTATTTA+AGG - Chr8:33382644-33382663 MsG0880043956.01.T01:intron 25.0%
! TTAGAATACACGTTAGAAAA+AGG - Chr8:33380984-33381003 MsG0880043956.01.T01:CDS 25.0%
! TTAGTAGTGTTTATATAGTG+GGG - Chr8:33381268-33381287 MsG0880043956.01.T01:intron 25.0%
! TTATTGTGTTAGTTAGTGTT+TGG + Chr8:33384672-33384691 None:intergenic 25.0%
! TTCAAGTAACTACAAAAATG+AGG - Chr8:33384333-33384352 MsG0880043956.01.T01:intron 25.0%
!! AGTAATAAATTTGGAATTGC+AGG + Chr8:33383984-33384003 None:intergenic 25.0%
!! ATTTTTACGTGAAATAATCG+GGG - Chr8:33383610-33383629 MsG0880043956.01.T01:intron 25.0%
!! GAACACAATCTGAAATATTT+GGG - Chr8:33381648-33381667 MsG0880043956.01.T01:intron 25.0%
!! GAGTTTAAGAGCATAAAAAA+TGG + Chr8:33384230-33384249 None:intergenic 25.0%
!! TTCTATCGAAGTTAGTATTT+TGG + Chr8:33382434-33382453 None:intergenic 25.0%
!!! AGGTTTGTGCATTATTTTTA+AGG - Chr8:33384382-33384401 MsG0880043956.01.T01:intron 25.0%
!!! ATATTTTTTTGCTGGAATGT+CGG + Chr8:33385510-33385529 None:intergenic 25.0%
!!! CATTTTTTAAATTCAAGTGG+GGG - Chr8:33381126-33381145 MsG0880043956.01.T01:intron 25.0%
!!! CCATTTTTTAAATTCAAGTG+GGG - Chr8:33381125-33381144 MsG0880043956.01.T01:intron 25.0%
!!! CTATTCTTTTTTTTTGGTAG+TGG + Chr8:33383468-33383487 None:intergenic 25.0%
!!! GAATGCAATCTAGAATATTT+TGG - Chr8:33381626-33381645 MsG0880043956.01.T01:intron 25.0%
!!! TCTTTTTAGTTTACGACTAT+TGG - Chr8:33384087-33384106 MsG0880043956.01.T01:intron 25.0%
!!! TTATTTTGTTAATCCATCCT+TGG - Chr8:33382511-33382530 MsG0880043956.01.T01:intron 25.0%
AAAATCACTTTCAAGCTCTT+CGG - Chr8:33385755-33385774 MsG0880043956.01.T01:intron 30.0%
AATAGATTCCTATGAGTTGT+CGG - Chr8:33383881-33383900 MsG0880043956.01.T01:intron 30.0%
AGATAAATGTTTCGCTTTAC+AGG - Chr8:33385285-33385304 MsG0880043956.01.T01:intron 30.0%
ATAAAAATATGCAAGGTTCG+GGG - Chr8:33383430-33383449 MsG0880043956.01.T01:intron 30.0%
ATATTGTAACCACTCTATTG+TGG + Chr8:33382176-33382195 None:intergenic 30.0%
CATAAAAATATGCAAGGTTC+GGG - Chr8:33383429-33383448 MsG0880043956.01.T01:intron 30.0%
CATCCTTGAAGTAATTGATA+AGG - Chr8:33385814-33385833 MsG0880043956.01.T01:intron 30.0%
CCCCACTTGAATTTAAAAAA+TGG + Chr8:33381128-33381147 None:intergenic 30.0%
CTAAACACAAATTCTCATGT+TGG - Chr8:33381184-33381203 MsG0880043956.01.T01:intron 30.0%
GAAAGGAAAAAAAAAGTGAG+AGG + Chr8:33384268-33384287 None:intergenic 30.0%
GAGTGGTTACAATATCATAT+TGG - Chr8:33382181-33382200 MsG0880043956.01.T01:intron 30.0%
GCATAAAAATATGCAAGGTT+CGG - Chr8:33383428-33383447 MsG0880043956.01.T01:intron 30.0%
GTATTTCAACCACTCAAATA+TGG + Chr8:33382087-33382106 None:intergenic 30.0%
GTTGCGTTAATTGAAAAAAG+TGG - Chr8:33381217-33381236 MsG0880043956.01.T01:intron 30.0%
TAACACACACATGTAAAACA+AGG - Chr8:33384750-33384769 MsG0880043956.01.T01:intron 30.0%
TAATTATGTACCCCTATTAG+AGG + Chr8:33381299-33381318 None:intergenic 30.0%
TAGTAGTGTTTATATAGTGG+GGG - Chr8:33381269-33381288 MsG0880043956.01.T01:intron 30.0%
TCTCCATAAGATTTACATAG+TGG + Chr8:33381081-33381100 None:intergenic 30.0%
TGGTTTCAGTATTAAAAGAG+TGG - Chr8:33382201-33382220 MsG0880043956.01.T01:intron 30.0%
TTTCTATCGAAGTTGTTACT+AGG + Chr8:33382396-33382415 None:intergenic 30.0%
TTTCTGTTTCAGTTTGTGTA+TGG - Chr8:33385569-33385588 MsG0880043956.01.T01:intron 30.0%
! AAATCTCTATTTAAGGCTTG+GGG - Chr8:33382651-33382670 MsG0880043956.01.T01:intron 30.0%
! ACAATCTGAAATATTTGGGT+AGG - Chr8:33381652-33381671 MsG0880043956.01.T01:intron 30.0%
! ATTCCCAAACTGTTGTATTT+TGG + Chr8:33382459-33382478 None:intergenic 30.0%
! CAAATCTCTATTTAAGGCTT+GGG - Chr8:33382650-33382669 MsG0880043956.01.T01:intron 30.0%
! GGAACACAATCTGAAATATT+TGG - Chr8:33381647-33381666 MsG0880043956.01.T01:intron 30.0%
! TTCACTGCTTATCCTTTTAT+AGG - Chr8:33385236-33385255 MsG0880043956.01.T01:intron 30.0%
! TTTTCTTCAGCAGAAAAAGT+TGG - Chr8:33382608-33382627 MsG0880043956.01.T01:intron 30.0%
!! AGTTTTCGTAAACTTGTTTC+AGG + Chr8:33384970-33384989 None:intergenic 30.0%
!! TTTCACAAAGGTTTTGTACA+TGG + Chr8:33382808-33382827 None:intergenic 30.0%
AAAGGAGTAGAAAAAGGAGA+AGG + Chr8:33381934-33381953 None:intergenic 35.0%
AAGTAGTCCATATAGCCATT+TGG + Chr8:33385905-33385924 None:intergenic 35.0%
AATGGCTAAGGAATGTGTAA+AGG + Chr8:33385483-33385502 None:intergenic 35.0%
AGACATTGATGAACACTACA+AGG - Chr8:33385781-33385800 MsG0880043956.01.T01:intron 35.0%
ATAATCAAGGTCTCTTTGCA+AGG + Chr8:33385614-33385633 None:intergenic 35.0%
ATATTACTACCACTTGATGG+TGG + Chr8:33382556-33382575 None:intergenic 35.0%
CAGACAGTATAGTGAATTCA+CGG + Chr8:33385216-33385235 None:intergenic 35.0%
CATGAAACTAAACTGCATCA+AGG - Chr8:33382833-33382852 MsG0880043956.01.T01:intron 35.0%
CTTGAAGTAATTGATAAGGC+CGG - Chr8:33385818-33385837 MsG0880043956.01.T01:intron 35.0%
GGAGAAGTTAGATGTTAGAA+TGG - Chr8:33382053-33382072 MsG0880043956.01.T01:intron 35.0%
GGATGCTTTAACACCTATTT+CGG + Chr8:33382747-33382766 None:intergenic 35.0%
GTGTTAAGACCACTCAAATA+TGG + Chr8:33382154-33382173 None:intergenic 35.0%
GTTAGAAAAAGGTTTCTGTG+TGG - Chr8:33380995-33381014 MsG0880043956.01.T01:CDS 35.0%
TAAACCGAAAAGGGACTATA+TGG + Chr8:33381989-33382008 None:intergenic 35.0%
TAAACTCACCTATACGAATG+TGG - Chr8:33380868-33380887 MsG0880043956.01.T01:CDS 35.0%
TAGTTTAAAGAACTTGGCAG+AGG + Chr8:33382768-33382787 None:intergenic 35.0%
TTACGAAAACTGCAATGAAG+AGG - Chr8:33384979-33384998 MsG0880043956.01.T01:intron 35.0%
TTTCAGGATAAACACTTGCT+TGG + Chr8:33384954-33384973 None:intergenic 35.0%
TTTCATGGACTCTTTCACAA+AGG + Chr8:33382820-33382839 None:intergenic 35.0%
! AAACAGTGGACTTATGTCAT+AGG - Chr8:33382884-33382903 MsG0880043956.01.T01:intron 35.0%
! AAGACCATATAGTCCCTTTT+CGG - Chr8:33381982-33382001 MsG0880043956.01.T01:intron 35.0%
! CTTGATGCAGTTTAGTTTCA+TGG + Chr8:33382835-33382854 None:intergenic 35.0%
! GCAAATCTCTATTTAAGGCT+TGG - Chr8:33382649-33382668 MsG0880043956.01.T01:intron 35.0%
! GCGTATTTCTATTGTTCTGT+GGG - Chr8:33385540-33385559 MsG0880043956.01.T01:intron 35.0%
! GGTAAAATTAGCACCGAAAT+AGG - Chr8:33382731-33382750 MsG0880043956.01.T01:intron 35.0%
! TGCGTATTTCTATTGTTCTG+TGG - Chr8:33385539-33385558 MsG0880043956.01.T01:intron 35.0%
! TTGCTTGTTTCTCCCAAAAA+TGG - Chr8:33382700-33382719 MsG0880043956.01.T01:intron 35.0%
!! AATGAAGAGGCATTCTTGTA+TGG - Chr8:33384992-33385011 MsG0880043956.01.T01:intron 35.0%
!! ATGAAGAGGCATTCTTGTAT+GGG - Chr8:33384993-33385012 MsG0880043956.01.T01:intron 35.0%
!! ATTTTTGGGAGAAACAAGCA+AGG + Chr8:33382701-33382720 None:intergenic 35.0%
!! TTTTTGGGAGAAACAAGCAA+GGG + Chr8:33382700-33382719 None:intergenic 35.0%
!!! GTGTCTTTGTTTTAGTCGTT+AGG - Chr8:33385258-33385277 MsG0880043956.01.T01:intron 35.0%
!!! TAGCTGAGTTTGTTTTATCC+TGG - Chr8:33382293-33382312 MsG0880043956.01.T01:intron 35.0%
!!! TCTTTTTTTTTGGTAGTGGC+CGG + Chr8:33383464-33383483 None:intergenic 35.0%
AAAAAGTGGAAGTCCCACAT+CGG - Chr8:33381231-33381250 MsG0880043956.01.T01:intron 40.0%
AAATTCAAGTGGGGGATTGT+TGG - Chr8:33381134-33381153 MsG0880043956.01.T01:intron 40.0%
AATGTCGGCATTAATGGCTA+AGG + Chr8:33385495-33385514 None:intergenic 40.0%
AGTTGAGTTCTGTGCGATTT+CGG - Chr8:33383036-33383055 MsG0880043956.01.T01:intron 40.0%
ATGCATTGTCCAACCAACTT+GGG + Chr8:33381522-33381541 None:intergenic 40.0%
CAAGGGATAATATGAGCAAC+TGG + Chr8:33382683-33382702 None:intergenic 40.0%
CCTATACGAATGTGGAACTA+AGG - Chr8:33380876-33380895 MsG0880043956.01.T01:CDS 40.0%
CCTTAGTTCCACATTCGTAT+AGG + Chr8:33380879-33380898 None:intergenic 40.0%
CGAGAATTACACCTTCGATA+TGG + Chr8:33382235-33382254 None:intergenic 40.0%
CTAAGATCACAAACACCACT+AGG - Chr8:33383154-33383173 MsG0880043956.01.T01:intron 40.0%
CTATGAGTTGTCGGTAACTA+AGG - Chr8:33383890-33383909 MsG0880043956.01.T01:intron 40.0%
CTTAGTTACCGACAACTCAT+AGG + Chr8:33383892-33383911 None:intergenic 40.0%
GAACAGACAGAGTGAATTCA+CGG + Chr8:33385189-33385208 None:intergenic 40.0%
GAGGATATTGATCCTCCAAA+TGG - Chr8:33385887-33385906 MsG0880043956.01.T01:CDS 40.0%
GCGTACGAAATAGTCGTTTA+AGG - Chr8:33384362-33384381 MsG0880043956.01.T01:intron 40.0%
GGACAGAAAGGAGTAGAAAA+AGG + Chr8:33381940-33381959 None:intergenic 40.0%
GTCATAGCACCATATTTGAG+TGG - Chr8:33382075-33382094 MsG0880043956.01.T01:intron 40.0%
GTCTTAACACCACAATAGAG+TGG - Chr8:33382164-33382183 MsG0880043956.01.T01:intron 40.0%
GTGGTAACACCATATTTGAG+TGG - Chr8:33382142-33382161 MsG0880043956.01.T01:intron 40.0%
GTGTATGGCTATCACTTCTT+TGG - Chr8:33385584-33385603 MsG0880043956.01.T01:intron 40.0%
TAGTCCATATAGCCATTTGG+AGG + Chr8:33385902-33385921 None:intergenic 40.0%
TATGCATTGTCCAACCAACT+TGG + Chr8:33381440-33381459 None:intergenic 40.0%
TCTAAAAGCCCGTGTGTTTA+TGG + Chr8:33380969-33380988 None:intergenic 40.0%
TGACTTCTATGCGTTAGTGT+GGG - Chr8:33384705-33384724 MsG0880043956.01.T01:intron 40.0%
TGATCCTCCAAATGGCTATA+TGG - Chr8:33385895-33385914 MsG0880043956.01.T01:CDS 40.0%
! AACCAACTAAGTTGACCTAG+TGG + Chr8:33383172-33383191 None:intergenic 40.0%
! AGTCTGCCTTGCACAATTTT+GGG - Chr8:33382332-33382351 MsG0880043956.01.T01:intron 40.0%
! ATCCATGTCTCTAGTGCTTT+TGG + Chr8:33385930-33385949 None:intergenic 40.0%
! CGGCCTTATCAATTACTTCA+AGG + Chr8:33385820-33385839 None:intergenic 40.0%
! CTAGAGCGTTCGTTAAAACT+GGG - Chr8:33380929-33380948 MsG0880043956.01.T01:CDS 40.0%
! GAAGCATCAACTGCGAATTT+CGG + Chr8:33381865-33381884 None:intergenic 40.0%
! TAGTCTGCCTTGCACAATTT+TGG - Chr8:33382331-33382350 MsG0880043956.01.T01:intron 40.0%
! TCGAAGGTGTAATTCTCGAA+TGG - Chr8:33382237-33382256 MsG0880043956.01.T01:intron 40.0%
! TCTAGAGCGTTCGTTAAAAC+TGG - Chr8:33380928-33380947 MsG0880043956.01.T01:CDS 40.0%
! TGGTCTTAGTACCATATCGA+AGG - Chr8:33382221-33382240 MsG0880043956.01.T01:intron 40.0%
! TGTTTTCTTAGTCCCCAAGT+TGG - Chr8:33381506-33381525 MsG0880043956.01.T01:intron 40.0%
!! GCACTGATTCAGTTAAACAG+TGG - Chr8:33382870-33382889 MsG0880043956.01.T01:intron 40.0%
!!! AGTTTGTTTTATCCTGGAGG+CGG - Chr8:33382299-33382318 MsG0880043956.01.T01:intron 40.0%
!!! CTGAGTTTGTTTTATCCTGG+AGG - Chr8:33382296-33382315 MsG0880043956.01.T01:intron 40.0%
!!! CTTTTTTTTTGGTAGTGGCC+GGG + Chr8:33383463-33383482 None:intergenic 40.0%
!!! GATTTTTTTGGTATTGGCCG+AGG + Chr8:33381474-33381493 None:intergenic 40.0%
!!! TTTTTTTTTGGTAGTGGCCG+GGG + Chr8:33383462-33383481 None:intergenic 40.0%
AAAACTGGGATAGACGTGCA+GGG - Chr8:33380943-33380962 MsG0880043956.01.T01:CDS 45.0%
AACTGCATCAAGGTGATGAC+TGG - Chr8:33382843-33382862 MsG0880043956.01.T01:intron 45.0%
AAGAACACTCTCGATTCCGA+AGG + Chr8:33381962-33381981 None:intergenic 45.0%
ACATCACAACACAGATGGCA+AGG - Chr8:33385685-33385704 MsG0880043956.01.T01:intron 45.0%
AGAACACTCTCGATTCCGAA+GGG + Chr8:33381961-33381980 None:intergenic 45.0%
AGACACACACACATGATCGA+TGG + Chr8:33384906-33384925 None:intergenic 45.0%
ATCACTTTCAAGCTCTTCGG+TGG - Chr8:33385758-33385777 MsG0880043956.01.T01:intron 45.0%
CAAACTCAGCTACAACTGTG+CGG + Chr8:33382285-33382304 None:intergenic 45.0%
CACACATGATCGATGGGAAA+AGG + Chr8:33384899-33384918 None:intergenic 45.0%
CACACTAACGCATAGAAGTC+AGG + Chr8:33384706-33384725 None:intergenic 45.0%
CACCACTAGGTCAACTTAGT+TGG - Chr8:33383167-33383186 MsG0880043956.01.T01:intron 45.0%
CATGGATGCTCATCTTGTCA+AGG - Chr8:33385943-33385962 MsG0880043956.01.T01:CDS 45.0%
CGCACAGAACTCAACTAACA+AGG + Chr8:33383032-33383051 None:intergenic 45.0%
CGGCAGAAGAAAGTCTATCA+TGG + Chr8:33385169-33385188 None:intergenic 45.0%
CTGACTTCTATGCGTTAGTG+TGG - Chr8:33384704-33384723 MsG0880043956.01.T01:intron 45.0%
CTGGAAAAGAAGACCAAGGA+TGG + Chr8:33382527-33382546 None:intergenic 45.0%
GAAGTAATTGATAAGGCCGG+TGG - Chr8:33385821-33385840 MsG0880043956.01.T01:intron 45.0%
GACACACACACATGATCGAT+GGG + Chr8:33384905-33384924 None:intergenic 45.0%
GACTTCTATGCGTTAGTGTG+GGG - Chr8:33384706-33384725 MsG0880043956.01.T01:intron 45.0%
GACTTGTCAACACAAGTCGA+AGG + Chr8:33381912-33381931 None:intergenic 45.0%
GGAGAAGAGAGGAAAAACAC+AGG + Chr8:33381887-33381906 None:intergenic 45.0%
GTGAACGAAGTAAGCACTAG+AGG - Chr8:33381320-33381339 MsG0880043956.01.T01:intron 45.0%
TAAAACTGGGATAGACGTGC+AGG - Chr8:33380942-33380961 MsG0880043956.01.T01:CDS 45.0%
TACGAATGTGGAACTAAGGC+CGG - Chr8:33380880-33380899 MsG0880043956.01.T01:CDS 45.0%
TATTCTGCCCCGAATTCCAT+AGG + Chr8:33380908-33380927 None:intergenic 45.0%
TCTACTCCTTTCTGTCCCTT+CGG - Chr8:33381943-33381962 MsG0880043956.01.T01:intron 45.0%
TGCATTGTCCAACCAACTTG+GGG + Chr8:33381521-33381540 None:intergenic 45.0%
TGCTGGAATGTCGGCATTAA+TGG + Chr8:33385501-33385520 None:intergenic 45.0%
TGTCAACACAAGTCGAAGGA+GGG + Chr8:33381908-33381927 None:intergenic 45.0%
TTGTCAACACAAGTCGAAGG+AGG + Chr8:33381909-33381928 None:intergenic 45.0%
TTTAACAGCAGCACTTCCAC+CGG + Chr8:33385840-33385859 None:intergenic 45.0%
! CAACTAAGTTGACCTAGTGG+TGG + Chr8:33383169-33383188 None:intergenic 45.0%
! TTACCAGCGAGCACCATTTT+TGG + Chr8:33382716-33382735 None:intergenic 45.0%
!! CATCCTTGGTCTTCTTTTCC+AGG - Chr8:33382525-33382544 MsG0880043956.01.T01:intron 45.0%
!! GCTCTCTTTAAGACGTTTCG+TGG + Chr8:33382363-33382382 None:intergenic 45.0%
!! GTGGAAGTGCTGCTGTTAAA+TGG - Chr8:33385840-33385859 MsG0880043956.01.T01:intron 45.0%
!! TACCAGCGAGCACCATTTTT+GGG + Chr8:33382715-33382734 None:intergenic 45.0%
!! TGCCAAAAGCACTAGAGACA+TGG - Chr8:33385925-33385944 MsG0880043956.01.T01:CDS 45.0%
!! TTACTACCACTTGATGGTGG+TGG + Chr8:33382553-33382572 None:intergenic 45.0%
!! TTCTTAGTCCCCAAGTTGGT+TGG - Chr8:33381510-33381529 MsG0880043956.01.T01:intron 45.0%
!!! GATTTTTTTGGTAGTGGCCG+AGG + Chr8:33381557-33381576 None:intergenic 45.0%
!!! GATTTTTTTTAATAATAAAA+AGG + Chr8:33385102-33385121 None:intergenic 5.0%
!!! TTGATATATATTTTATTATT+TGG - Chr8:33381356-33381375 MsG0880043956.01.T01:intron 5.0%
CATCAAGGTGATGACTGGCA+TGG - Chr8:33382848-33382867 MsG0880043956.01.T01:intron 50.0%
CCACGACATCACAACACAGA+TGG - Chr8:33385680-33385699 MsG0880043956.01.T01:intron 50.0%
CCATCTGTGTTGTGATGTCG+TGG + Chr8:33385683-33385702 None:intergenic 50.0%
CCTCAACAAACACCACCACT+AGG - Chr8:33383210-33383229 MsG0880043956.01.T01:intron 50.0%
GAGCCTGGAAAAGAAGACCA+AGG + Chr8:33382531-33382550 None:intergenic 50.0%
GCACTGCCCAAAATTGTGCA+AGG + Chr8:33382341-33382360 None:intergenic 50.0%
GGAGTGGAGTACATAGCTAC+AGG - Chr8:33380834-33380853 MsG0880043956.01.T01:CDS 50.0%
GGGGATTGTTGGAACATGTG+CGG - Chr8:33381145-33381164 MsG0880043956.01.T01:intron 50.0%
GTGAGCTTAGCTCAGTTGGT+TGG - Chr8:33381427-33381446 MsG0880043956.01.T01:intron 50.0%
GTGTGTGTTCCATCCGATGT+GGG + Chr8:33381247-33381266 None:intergenic 50.0%
TCGATTCCGAAGGGACAGAA+AGG + Chr8:33381952-33381971 None:intergenic 50.0%
TCTATGCGTTAGTGTGGGGT+GGG - Chr8:33384710-33384729 MsG0880043956.01.T01:intron 50.0%
TGTGTGTGTTCCATCCGATG+TGG + Chr8:33381248-33381267 None:intergenic 50.0%
TTCTATGCGTTAGTGTGGGG+TGG - Chr8:33384709-33384728 MsG0880043956.01.T01:intron 50.0%
! AGGCCGGTTCCTATGGAATT+CGG - Chr8:33380896-33380915 MsG0880043956.01.T01:CDS 50.0%
! AGTGGGGGTGTACCTCTAAT+AGG - Chr8:33381284-33381303 MsG0880043956.01.T01:intron 50.0%
! CCTAGTGGTGGTGTTTGTTG+AGG + Chr8:33383213-33383232 None:intergenic 50.0%
! GTGGGGGTGTACCTCTAATA+GGG - Chr8:33381285-33381304 MsG0880043956.01.T01:intron 50.0%
! TGGGGGTGTACCTCTAATAG+GGG - Chr8:33381286-33381305 MsG0880043956.01.T01:intron 50.0%
AAGGTTCGGGGTTCAAACCC+CGG - Chr8:33383442-33383461 MsG0880043956.01.T01:intron 55.0%
AGTCGAAGGAGGGAGAAGAG+AGG + Chr8:33381898-33381917 None:intergenic 55.0%
AGTGGAAGTCCCACATCGGA+TGG - Chr8:33381235-33381254 MsG0880043956.01.T01:intron 55.0%
CCAACTGAGCTAAGCTCACG+AGG + Chr8:33381426-33381445 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - Chr8:33381423-33381442 MsG0880043956.01.T01:intron 55.0%
CGTGCAGGGCCATAAACACA+CGG - Chr8:33380957-33380976 MsG0880043956.01.T01:CDS 55.0%
CTATGCGTTAGTGTGGGGTG+GGG - Chr8:33384711-33384730 MsG0880043956.01.T01:intron 55.0%
CTCCCAAAAATGGTGCTCGC+TGG - Chr8:33382710-33382729 MsG0880043956.01.T01:intron 55.0%
GCCCCGAATTCCATAGGAAC+CGG + Chr8:33380902-33380921 None:intergenic 55.0%
GTGCAGGGCCATAAACACAC+GGG - Chr8:33380958-33380977 MsG0880043956.01.T01:CDS 55.0%
! GCCGGTTCCTATGGAATTCG+GGG - Chr8:33380898-33380917 MsG0880043956.01.T01:CDS 55.0%
! GGAACTAAGGCCGGTTCCTA+TGG - Chr8:33380889-33380908 MsG0880043956.01.T01:CDS 55.0%
! GGCCGGTTCCTATGGAATTC+GGG - Chr8:33380897-33380916 MsG0880043956.01.T01:CDS 55.0%
! GTTTTATCCTGGAGGCGGCA+TGG - Chr8:33382304-33382323 MsG0880043956.01.T01:intron 55.0%
CAGGCTCCACCACCATCAAG+TGG - Chr8:33382544-33382563 MsG0880043956.01.T01:intron 60.0%
CTATCAACCATGCCGCCTCC+AGG + Chr8:33382314-33382333 None:intergenic 60.0%
!! CACTTGATGGTGGTGGAGCC+TGG + Chr8:33382546-33382565 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33380830 33385970 33380830 ID=MsG0880043956.01;Name=MsG0880043956.01
Chr8 mRNA 33380830 33385970 33380830 ID=MsG0880043956.01.T01;Parent=MsG0880043956.01;Name=MsG0880043956.01.T01;_AED=0.50;_eAED=0.64;_QI=0|0|0|1|1|1|2|0|125
Chr8 exon 33385865 33385970 33385865 ID=MsG0880043956.01.T01:exon:10184;Parent=MsG0880043956.01.T01
Chr8 exon 33380830 33381101 33380830 ID=MsG0880043956.01.T01:exon:10183;Parent=MsG0880043956.01.T01
Chr8 CDS 33385865 33385970 33385865 ID=MsG0880043956.01.T01:cds;Parent=MsG0880043956.01.T01
Chr8 CDS 33380830 33381101 33380830 ID=MsG0880043956.01.T01:cds;Parent=MsG0880043956.01.T01
Gene Sequence

>MsG0880043956.01.T01

ATGTGGAGTGGAGTACATAGCTACAGGAGTACTCTTGATAAACTCACCTATACGAATGTGGAACTAAGGCCGGTTCCTATGGAATTCGGGGCAGAATATCTAGAGCATGGCAAGGTACACACATGTCATGAGATTATTGAAGATCTTGATAAACAGAACAAAAAAATCACTTTCAAGCTCTTCGGTGGAGACATTGATGAACACTACAAGGTCTTTAAGATCATCCTTGAAGTAATTGATAAGGCCGGTGGAAGTGCTGCTGTTAAATGGACTATTGAATATGAGAAAATCAATGAGGATATTGATCCTCCAAATGGCTATATGGACTACTTTGCCAAAAGCACTAGAGACATGGATGCTCATCTTGTCAAGGCTTAA
Protein sequence

>MsG0880043956.01.T01

MWSGVHSYRSTLDKLTYTNVELRPVPMEFGAEYLEHGKVHTCHEIIEDLDKQNKKITFKLFGGDIDEHYKVFKIILEVIDKAGGSAAVKWTIEYEKINEDIDPPNGYMDYFAKSTRDMDAHLVKA*