Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043966.01.T01 | XP_039683451.1 | 92.727 | 55 | 4 | 0 | 1 | 55 | 76 | 130 | 1.44E-25 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043966.01.T01 | A0A072TGZ8 | 90.909 | 55 | 5 | 0 | 1 | 55 | 76 | 130 | 1.07e-24 | 97.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780036080.01 | MsG0880043966.01 | 0.807814 | 4.206062e-50 | 2.365732e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043966.01.T01 | MTR_0125s0060 | 90.909 | 55 | 5 | 0 | 1 | 55 | 76 | 130 | 2.72e-28 | 97.8 |
| MsG0880043966.01.T01 | MTR_2g035110 | 81.667 | 60 | 11 | 0 | 1 | 60 | 76 | 135 | 1.75e-27 | 95.9 |
| MsG0880043966.01.T01 | MTR_8g066845 | 67.213 | 61 | 18 | 2 | 1 | 60 | 28 | 87 | 7.14e-18 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 8:+33522300 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 8:+33522276 | None:intergenic |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.408396 | 8:-33522333 | MsG0880043966.01.T01:CDS |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 8:-33522276 | MsG0880043966.01.T01:CDS |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 8:-33522355 | MsG0880043966.01.T01:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 8:-33522346 | MsG0880043966.01.T01:CDS |
| AGCGCTTGGAGCAAATGATT+GGG | 0.526657 | 8:-33522332 | MsG0880043966.01.T01:CDS |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 8:-33522295 | MsG0880043966.01.T01:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 8:-33522285 | MsG0880043966.01.T01:CDS |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 8:-33522291 | MsG0880043966.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTGAAGTGTTCATATT+AGG | + | Chr8:33522365-33522384 | None:intergenic | 25.0% |
| AGAAACGCTAAAACTGAAGA+TGG | - | Chr8:33522307-33522326 | MsG0880043966.01.T01:CDS | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | - | Chr8:33522367-33522386 | MsG0880043966.01.T01:CDS | 35.0% | |
| AACACTTCAATTCAGCCGGA+AGG | - | Chr8:33522371-33522390 | MsG0880043966.01.T01:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | - | Chr8:33522330-33522349 | MsG0880043966.01.T01:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | + | Chr8:33522389-33522408 | None:intergenic | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | - | Chr8:33522316-33522335 | MsG0880043966.01.T01:CDS | 45.0% |
| CCGGAAGGAGAAGGTGATAA+TGG | - | Chr8:33522386-33522405 | MsG0880043966.01.T01:CDS | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | - | Chr8:33522329-33522348 | MsG0880043966.01.T01:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | - | Chr8:33522377-33522396 | MsG0880043966.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 33522251 | 33522433 | 33522251 | ID=MsG0880043966.01;Name=MsG0880043966.01 |
| Chr8 | mRNA | 33522251 | 33522433 | 33522251 | ID=MsG0880043966.01.T01;Parent=MsG0880043966.01;Name=MsG0880043966.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|60 |
| Chr8 | exon | 33522251 | 33522433 | 33522251 | ID=MsG0880043966.01.T01:exon:2978;Parent=MsG0880043966.01.T01 |
| Chr8 | CDS | 33522251 | 33522433 | 33522251 | ID=MsG0880043966.01.T01:cds;Parent=MsG0880043966.01.T01 |
| Gene Sequence |
| Protein sequence |
