AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036080.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036080.01.T01 MTR_0125s0060 82.716 81 14 0 1 81 1 81 2.59e-44 139
MsG0780036080.01.T01 MTR_2g035110 71.605 81 23 0 1 81 1 81 1.53e-35 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGACAATGATATTTATA+TGG 0.247121 7:-2151381 MsG0780036080.01.T01:CDS
AACAAGAAGGGGCGCATATT+TGG 0.259894 7:-2151341 MsG0780036080.01.T01:CDS
AAGGGGCGCATATTTGGATT+AGG 0.373630 7:-2151335 MsG0780036080.01.T01:CDS
GATATTTATATGGAAATTGT+TGG 0.391812 7:-2151371 MsG0780036080.01.T01:CDS
GTTTCTCAAACGTTTGAGTT+TGG 0.397323 7:+2151447 None:intergenic
TGTTGGAGGCATTAACAAGA+AGG 0.515610 7:-2151354 MsG0780036080.01.T01:CDS
GCTACATCAGTAGAAATTGA+AGG 0.516389 7:+2151272 None:intergenic
ATTTATATGGAAATTGTTGG+AGG 0.522832 7:-2151368 MsG0780036080.01.T01:CDS
TCATTGTCAATTTCTTCAGT+AGG 0.525205 7:+2151392 None:intergenic
GTTGGAGGCATTAACAAGAA+GGG 0.555037 7:-2151353 MsG0780036080.01.T01:CDS
TTGGAGGCATTAACAAGAAG+GGG 0.580609 7:-2151352 MsG0780036080.01.T01:CDS
TCAGTAGAAATTGAAGGCGA+TGG 0.593473 7:+2151278 None:intergenic
TTTGAGAAACACTCTCAAAG+TGG 0.627086 7:-2151434 MsG0780036080.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGACAATGATATTTATA+TGG - Chr7:2151297-2151316 MsG0780036080.01.T01:CDS 15.0%
!! GATATTTATATGGAAATTGT+TGG - Chr7:2151307-2151326 MsG0780036080.01.T01:CDS 20.0%
! ATTTATATGGAAATTGTTGG+AGG - Chr7:2151310-2151329 MsG0780036080.01.T01:CDS 25.0%
TCATTGTCAATTTCTTCAGT+AGG + Chr7:2151289-2151308 None:intergenic 30.0%
GCTACATCAGTAGAAATTGA+AGG + Chr7:2151409-2151428 None:intergenic 35.0%
TTTGAGAAACACTCTCAAAG+TGG - Chr7:2151244-2151263 MsG0780036080.01.T01:CDS 35.0%
! GTTTCTCAAACGTTTGAGTT+TGG + Chr7:2151234-2151253 None:intergenic 35.0%
CTTCGAAAAAGTTGCTGCTA+TGG - Chr7:2151431-2151450 MsG0780036080.01.T01:CDS 40.0%
TCAGTAGAAATTGAAGGCGA+TGG + Chr7:2151403-2151422 None:intergenic 40.0%
!! GTTGGAGGCATTAACAAGAA+GGG - Chr7:2151325-2151344 MsG0780036080.01.T01:CDS 40.0%
!! TGTTGGAGGCATTAACAAGA+AGG - Chr7:2151324-2151343 MsG0780036080.01.T01:CDS 40.0%
!! TTGGAGGCATTAACAAGAAG+GGG - Chr7:2151326-2151345 MsG0780036080.01.T01:CDS 40.0%
CGAAAAAGTTGCTGCTATGG+AGG - Chr7:2151434-2151453 MsG0780036080.01.T01:CDS 45.0%
! AACAAGAAGGGGCGCATATT+TGG - Chr7:2151337-2151356 MsG0780036080.01.T01:CDS 45.0%
! AAGGGGCGCATATTTGGATT+AGG - Chr7:2151343-2151362 MsG0780036080.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 2151226 2151474 2151226 ID=MsG0780036080.01;Name=MsG0780036080.01
Chr7 mRNA 2151226 2151474 2151226 ID=MsG0780036080.01.T01;Parent=MsG0780036080.01;Name=MsG0780036080.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|82
Chr7 exon 2151226 2151474 2151226 ID=MsG0780036080.01.T01:exon:38497;Parent=MsG0780036080.01.T01
Chr7 CDS 2151226 2151474 2151226 ID=MsG0780036080.01.T01:cds;Parent=MsG0780036080.01.T01
Gene Sequence

>MsG0780036080.01.T01

ATGTACCAAACTCAAACGTTTGAGAAACACTCTCAAAGTGGTGAGATTAGTAGTCAACAACCTACTGAAGAAATTGACAATGATATTTATATGGAAATTGTTGGAGGCATTAACAAGAAGGGGCGCATATTTGGATTAGGATCTCAAGCTGTTGTTGTCAAAGAATCTTTGAGACCATCGCCTTCAATTTCTACTGATGTAGCTTCTTCGAAAAAGTTGCTGCTATGGAGGCTAAGATTGAAGCATTAA

Protein sequence

>MsG0780036080.01.T01

MYQTQTFEKHSQSGEISSQQPTEEIDNDIYMEIVGGINKKGRIFGLGSQAVVVKESLRPSPSISTDVASSKKLLLWRLRLKH*