AlfalfaGEDB | MsG0880044111.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044111.01

KEGG: K00799
GO: -
PFAM: PF13410

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044111.01.T01	XP_003628404.1	93.043	115	8	0	14	128	105	219	4.19E-71	222

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044111.01.T01	P49332	58.929	112	46	0	17	128	110	221	3.22E-40	136

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044111.01.T01	G7LB15	93.043	115	8	0	14	128	105	219	2.00e-71	222

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001105.01	MsG0880044111.01	0.814974	1.172266e-51	7.980302e-49
MsG0880044109.01	MsG0880044111.01	0.936229	2.425768e-97	2.045527e-92
MsG0880044111.01	MsG0880044133.01	0.829350	5.406272e-55	5.519736e-52
MsG0180005292.01	MsG0880044111.01	0.801617	8.272579e-49	3.968477e-46
MsG0680034766.01	MsG0880044111.01	0.811152	8.076470e-51	4.961766e-48
MsG0680034767.01	MsG0880044111.01	0.813239	2.830946e-51	1.838576e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044111.01.T01	MTR_8g056940	93.043	115	8	0	14	128	105	219	5.07e-75	222
MsG0880044111.01.T01	MTR_2g070140	55.652	115	51	0	14	128	89	203	3.32e-40	133
MsG0880044111.01.T01	MTR_2g070060	54.783	115	52	0	14	128	105	219	1.07e-39	132
MsG0880044111.01.T01	MTR_4g124130	57.391	115	49	0	14	128	106	220	1.12e-39	132
MsG0880044111.01.T01	MTR_3g467420	50.435	115	57	0	14	128	110	224	6.47e-39	130
MsG0880044111.01.T01	MTR_3g467430	51.304	115	56	0	14	128	110	224	8.39e-39	130
MsG0880044111.01.T01	MTR_2g070180	55.455	110	49	0	14	123	105	214	8.11e-37	125
MsG0880044111.01.T01	MTR_2g070070	56.522	115	50	0	14	128	105	219	1.07e-36	125
MsG0880044111.01.T01	MTR_2g070210	51.818	110	53	0	14	123	107	216	1.42e-34	119
MsG0880044111.01.T01	MTR_2g070150	58.163	98	41	0	14	111	105	202	4.61e-34	117
MsG0880044111.01.T01	MTR_2g070110	58.696	92	38	0	37	128	127	218	7.85e-34	117
MsG0880044111.01.T01	MTR_2g070200	52.632	114	52	2	14	125	105	218	1.72e-32	114
MsG0880044111.01.T01	MTR_5g076900	50.909	110	54	0	14	123	107	216	2.54e-32	114
MsG0880044111.01.T01	MTR_2g070130	46.087	115	62	0	14	128	105	219	1.01e-31	112
MsG0880044111.01.T01	MTR_2g070120	43.636	110	62	0	13	122	104	213	2.12e-26	98.6
MsG0880044111.01.T01	MTR_4g043640	86.047	43	6	0	57	99	44	86	5.26e-23	85.9
MsG0880044111.01.T01	MTR_0186s0030	94.595	37	2	0	14	50	105	141	8.76e-17	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044111.01.T01	AT1G78380	55.963	109	48	0	17	125	108	216	1.99e-37	127
MsG0880044111.01.T01	AT1G78360	53.636	110	51	0	19	128	111	220	2.86e-36	124
MsG0880044111.01.T01	AT1G78370	53.704	108	50	0	17	124	108	215	5.53e-36	123
MsG0880044111.01.T01	AT1G17180	56.075	107	47	0	22	128	113	219	6.69e-36	123
MsG0880044111.01.T01	AT1G17170	54.237	118	51	2	11	128	104	218	3.24e-34	118
MsG0880044111.01.T01	AT1G78340	55.140	107	48	0	17	123	108	214	7.22e-34	117
MsG0880044111.01.T01	AT1G53680	52.294	109	50	2	19	125	113	221	1.26e-32	114
MsG0880044111.01.T01	AT1G17190	44.954	109	60	0	17	125	109	217	6.73e-28	102
MsG0880044111.01.T01	AT1G78320	50.000	102	51	0	22	123	86	187	1.75e-27	100
MsG0880044111.01.T01	AT1G78320	50.000	102	51	0	22	123	113	214	4.50e-27	100
MsG0880044111.01.T01	AT3G43800	41.739	115	66	1	14	127	109	223	2.49e-23	90.9

Find 23 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCAGACCAACCTTATTT+TGG	0.254425	8:+35673205	MsG0880044111.01.T01:CDS
GGAGTTGATTGAAGGCTTAA+AGG	0.261757	8:+35673168	MsG0880044111.01.T01:CDS
TTTCTCATCCGCAGCAGATT+TGG	0.264315	8:-35673377	None:intergenic
CTTTGATCCCTTTCTATAGT+TGG	0.290757	8:+35673257	MsG0880044111.01.T01:CDS
GTGATGTTGGAGGAAGGATA+TGG	0.346197	8:+35673110	MsG0880044111.01.T01:CDS
AAAGAGAGATGAGATTGAAT+TGG	0.418834	8:+35673138	MsG0880044111.01.T01:CDS
AATGTGTCACCACCAAAATA+AGG	0.439985	8:-35673217	None:intergenic
TGAGATTGAATTGGCAAAGA+AGG	0.443100	8:+35673147	MsG0880044111.01.T01:CDS
AAAGTGCAGGTTGGTGATGT+TGG	0.443577	8:+35673097	MsG0880044111.01.T01:CDS
CTATAGGAAGAGGTTTGAGT+TGG	0.476204	8:+35673423	MsG0880044111.01.T01:CDS
GCAAAGAAGGAGTTGATTGA+AGG	0.480756	8:+35673160	MsG0880044111.01.T01:CDS
TGATGTTGGAGGAAGGATAT+GGG	0.485671	8:+35673111	MsG0880044111.01.T01:CDS
TATAAAACCAACTATAGAAA+GGG	0.488530	8:-35673264	None:intergenic
GTATAAAACCAACTATAGAA+AGG	0.503827	8:-35673265	None:intergenic
GTGTGATAAGTTGATTGCTT+GGG	0.528271	8:+35673324	MsG0880044111.01.T01:CDS
ATTATGCAACTTCAAAGTTG+AGG	0.546513	8:+35673297	MsG0880044111.01.T01:CDS
GTTGGAGGAAGGATATGGGC+TGG	0.551186	8:+35673115	MsG0880044111.01.T01:CDS
TTGTTGTTGACTATAGGAAG+AGG	0.553068	8:+35673413	MsG0880044111.01.T01:CDS
AGTGTGATAAGTTGATTGCT+TGG	0.574483	8:+35673323	MsG0880044111.01.T01:CDS
GTGCAGGTTGGTGATGTTGG+AGG	0.591902	8:+35673100	MsG0880044111.01.T01:CDS
TGTCACCACCAAAATAAGGT+TGG	0.621870	8:-35673213	None:intergenic
AGGTTGGTGATGTTGGAGGA+AGG	0.642797	8:+35673104	MsG0880044111.01.T01:CDS
GAGTGTTTCCAAATCTGCTG+CGG	0.660347	8:+35673369	MsG0880044111.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTATTCATTTTAATG+TGG	+	Chr8:35673069-35673088	MsG0880044111.01.T01:CDS	10.0%
!!	TATAAAACCAACTATAGAAA+GGG	-	Chr8:35673267-35673286	None:intergenic	20.0%
!	GTATAAAACCAACTATAGAA+AGG	-	Chr8:35673268-35673287	None:intergenic	25.0%
!!!	ATGATTTTGTTGTTGACTAT+AGG	+	Chr8:35673407-35673426	MsG0880044111.01.T01:CDS	25.0%
	AAAGAGAGATGAGATTGAAT+TGG	+	Chr8:35673138-35673157	MsG0880044111.01.T01:CDS	30.0%
	ATTATGCAACTTCAAAGTTG+AGG	+	Chr8:35673297-35673316	MsG0880044111.01.T01:CDS	30.0%
	AATGTGTCACCACCAAAATA+AGG	-	Chr8:35673220-35673239	None:intergenic	35.0%
	CTTTGATCCCTTTCTATAGT+TGG	+	Chr8:35673257-35673276	MsG0880044111.01.T01:CDS	35.0%
	TGAGATTGAATTGGCAAAGA+AGG	+	Chr8:35673147-35673166	MsG0880044111.01.T01:CDS	35.0%
!	TAATGTGGTGAAAAAAGTGC+AGG	+	Chr8:35673084-35673103	MsG0880044111.01.T01:CDS	35.0%
!	TTGTTGTTGACTATAGGAAG+AGG	+	Chr8:35673413-35673432	MsG0880044111.01.T01:CDS	35.0%
!!	AGTGTGATAAGTTGATTGCT+TGG	+	Chr8:35673323-35673342	MsG0880044111.01.T01:CDS	35.0%
!!	GTGTGATAAGTTGATTGCTT+GGG	+	Chr8:35673324-35673343	MsG0880044111.01.T01:CDS	35.0%
!!!	ATTTTGGTGGTGACACATTT+GGG	+	Chr8:35673221-35673240	MsG0880044111.01.T01:CDS	35.0%
!!!	TATTTTGGTGGTGACACATT+TGG	+	Chr8:35673220-35673239	MsG0880044111.01.T01:CDS	35.0%
	CTATAGGAAGAGGTTTGAGT+TGG	+	Chr8:35673423-35673442	MsG0880044111.01.T01:CDS	40.0%
	TGATGTTGGAGGAAGGATAT+GGG	+	Chr8:35673111-35673130	MsG0880044111.01.T01:CDS	40.0%
	TGTCACCACCAAAATAAGGT+TGG	-	Chr8:35673216-35673235	None:intergenic	40.0%
!	GCAAAGAAGGAGTTGATTGA+AGG	+	Chr8:35673160-35673179	MsG0880044111.01.T01:CDS	40.0%
!	GGAGTTGATTGAAGGCTTAA+AGG	+	Chr8:35673168-35673187	MsG0880044111.01.T01:CDS	40.0%
!!	CTTGCAGACCAACCTTATTT+TGG	+	Chr8:35673205-35673224	MsG0880044111.01.T01:CDS	40.0%
	GAGTGTTTCCAAATCTGCTG+CGG	+	Chr8:35673369-35673388	MsG0880044111.01.T01:CDS	45.0%
	GTGATGTTGGAGGAAGGATA+TGG	+	Chr8:35673110-35673129	MsG0880044111.01.T01:CDS	45.0%
	TTTCTCATCCGCAGCAGATT+TGG	-	Chr8:35673380-35673399	None:intergenic	45.0%
!	GTGGTGAAAAAAGTGCAGGT+TGG	+	Chr8:35673088-35673107	MsG0880044111.01.T01:CDS	45.0%
!!	AAAGTGCAGGTTGGTGATGT+TGG	+	Chr8:35673097-35673116	MsG0880044111.01.T01:CDS	45.0%
!!!	GCAGACCAACCTTATTTTGG+TGG	+	Chr8:35673208-35673227	MsG0880044111.01.T01:CDS	45.0%
!!	AGGTTGGTGATGTTGGAGGA+AGG	+	Chr8:35673104-35673123	MsG0880044111.01.T01:CDS	50.0%
	GTTGGAGGAAGGATATGGGC+TGG	+	Chr8:35673115-35673134	MsG0880044111.01.T01:CDS	55.0%
!!	GTGCAGGTTGGTGATGTTGG+AGG	+	Chr8:35673100-35673119	MsG0880044111.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	35673064	35673478	35673064	ID=MsG0880044111.01;Name=MsG0880044111.01
Chr8	mRNA	35673064	35673450	35673064	ID=MsG0880044111.01.T01;Parent=MsG0880044111.01;Name=MsG0880044111.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|128
Chr8	exon	35673064	35673450	35673064	ID=MsG0880044111.01.T01:exon:5409;Parent=MsG0880044111.01.T01
Chr8	CDS	35673064	35673450	35673064	ID=MsG0880044111.01.T01:cds;Parent=MsG0880044111.01.T01
Chr8	mRNA	35673392	35673478	35673392	ID=MsG0880044111.01.T02;Parent=MsG0880044111.01;Name=MsG0880044111.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|28
Chr8	exon	35673392	35673478	35673392	ID=MsG0880044111.01.T02:exon:5410;Parent=MsG0880044111.01.T02
Chr8	CDS	35673392	35673478	35673392	ID=MsG0880044111.01.T02:cds;Parent=MsG0880044111.01.T02
Chr8	mRNA	35673200	35673262	35673200	ID=MsG0880044111.01.T03;Parent=MsG0880044111.01;Name=MsG0880044111.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|20
Chr8	exon	35673200	35673262	35673200	ID=MsG0880044111.01.T03:exon:5411;Parent=MsG0880044111.01.T03
Chr8	CDS	35673200	35673262	35673200	ID=MsG0880044111.01.T03:cds;Parent=MsG0880044111.01.T03
Chr8	mRNA	35673113	35673175	35673113	ID=MsG0880044111.01.T04;Parent=MsG0880044111.01;Name=MsG0880044111.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|20
Chr8	exon	35673113	35673175	35673113	ID=MsG0880044111.01.T04:exon:5412;Parent=MsG0880044111.01.T04
Chr8	CDS	35673113	35673175	35673113	ID=MsG0880044111.01.T04:cds;Parent=MsG0880044111.01.T04
Chr8	mRNA	35673290	35673337	35673290	ID=MsG0880044111.01.T05;Parent=MsG0880044111.01;Name=MsG0880044111.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|15
Chr8	exon	35673290	35673337	35673290	ID=MsG0880044111.01.T05:exon:5413;Parent=MsG0880044111.01.T05
Chr8	CDS	35673290	35673337	35673290	ID=MsG0880044111.01.T05:cds;Parent=MsG0880044111.01.T05

Gene Sequence

>MsG0880044111.01.T03

ATGTGCTTGCAGACCAACCTTATTTTGGTGGTGACACATTTGGGTTTGTTGACATTGCTTTGA

>MsG0880044111.01.T05

ATGAGAAATTATGCAACTTCAAAGTTGAGGAAGAGTGTGATAAGTTGA

>MsG0880044111.01.T02

ATGAGAAAGAAGTGTATGATTTTGTTGTTGACTATAGGAAGAGGTTTGAGTTGGACTGAGAAGACACTTCAAACTTTGAGAAATTGA

>MsG0880044111.01.T04

ATGTTGGAGGAAGGATATGGGCTGGAAAGAGAGATGAGATTGAATTGGCAAAGAAGGAGTTGA

>MsG0880044111.01.T01

ATGCTAATTTTTATTCATTTTAATGTGGTGAAAAAAGTGCAGGTTGGTGATGTTGGAGGAAGGATATGGGCTGGAAAGAGAGATGAGATTGAATTGGCAAAGAAGGAGTTGATTGAAGGCTTAAAGGAATTAGAGAATGTGCTTGCAGACCAACCTTATTTTGGTGGTGACACATTTGGGTTTGTTGACATTGCTTTGATCCCTTTCTATAGTTGGTTTTATACTTATGAGAAATTATGCAACTTCAAAGTTGAGGAAGAGTGTGATAAGTTGATTGCTTGGGCAAAGAGATGCAAGCAAAGAGAGAGTGTTTCCAAATCTGCTGCGGATGAGAAAGAAGTGTATGATTTTGTTGTTGACTATAGGAAGAGGTTTGAGTTGGACTGA

Protein sequence

>MsG0880044111.01.T03

MCLQTNLILVVTHLGLLTLL*

>MsG0880044111.01.T05

MRNYATSKLRKSVIS*

>MsG0880044111.01.T02

MRKKCMILLLTIGRGLSWTEKTLQTLRN*

>MsG0880044111.01.T04

MLEEGYGLEREMRLNWQRRS*

>MsG0880044111.01.T01

MLIFIHFNVVKKVQVGDVGGRIWAGKRDEIELAKKELIEGLKELENVLADQPYFGGDTFGFVDIALIPFYSWFYTYEKLCNFKVEEECDKLIAWAKRCKQRESVSKSAADEKEVYDFVVDYRKRFELD*