Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044345.01.T01 | XP_039685204.1 | 95.154 | 454 | 22 | 0 | 1 | 454 | 1 | 454 | 0 | 904 |
| MsG0880044345.01.T02 | XP_039685204.1 | 95 | 400 | 20 | 0 | 1 | 400 | 55 | 454 | 0 | 798 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044345.01.T01 | A0A396GL15 | 95.154 | 454 | 22 | 0 | 1 | 454 | 1 | 454 | 0.0 | 904 |
| MsG0880044345.01.T02 | A0A396GL15 | 95.000 | 400 | 20 | 0 | 1 | 400 | 55 | 454 | 0.0 | 798 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044345.01.T01 | MTR_8g058400 | 94.574 | 258 | 14 | 0 | 1 | 258 | 1 | 258 | 0.0 | 509 |
| MsG0880044345.01.T01 | MTR_8g059505 | 94.215 | 121 | 7 | 0 | 334 | 454 | 14 | 134 | 1.82e-79 | 243 |
| MsG0880044345.01.T01 | MTR_8g059505 | 95.455 | 110 | 5 | 0 | 345 | 454 | 1 | 110 | 4.59e-71 | 220 |
| MsG0880044345.01.T01 | MTR_3g100600 | 29.047 | 451 | 306 | 9 | 14 | 454 | 102 | 548 | 1.80e-57 | 199 |
| MsG0880044345.01.T01 | MTR_7g060720 | 28.474 | 439 | 299 | 6 | 7 | 434 | 47 | 481 | 6.68e-52 | 183 |
| MsG0880044345.01.T01 | MTR_7g060720 | 26.049 | 453 | 281 | 10 | 30 | 436 | 4 | 448 | 1.09e-30 | 124 |
| MsG0880044345.01.T01 | MTR_7g060720 | 24.599 | 187 | 138 | 1 | 256 | 439 | 125 | 311 | 8.82e-12 | 67.4 |
| MsG0880044345.01.T01 | MTR_4g108650 | 27.651 | 481 | 303 | 8 | 4 | 442 | 2 | 479 | 1.81e-51 | 186 |
| MsG0880044345.01.T01 | MTR_4g108650 | 28.142 | 366 | 255 | 4 | 80 | 440 | 185 | 547 | 1.04e-42 | 161 |
| MsG0880044345.01.T01 | MTR_4g108650 | 26.136 | 352 | 249 | 6 | 93 | 437 | 268 | 615 | 2.33e-38 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044345.01.T01 | AT1G53330 | 46.413 | 446 | 229 | 3 | 1 | 437 | 1 | 445 | 2.12e-138 | 405 |
| MsG0880044345.01.T01 | AT1G53330 | 46.921 | 341 | 178 | 2 | 99 | 437 | 2 | 341 | 4.23e-105 | 317 |
| MsG0880044345.01.T01 | AT1G64583 | 32.484 | 314 | 206 | 5 | 109 | 418 | 69 | 380 | 2.71e-49 | 176 |
| MsG0880044345.01.T01 | AT1G64583 | 28.916 | 332 | 232 | 3 | 109 | 437 | 139 | 469 | 1.48e-35 | 138 |
| MsG0880044345.01.T01 | AT1G64583 | 26.316 | 304 | 219 | 4 | 152 | 452 | 42 | 343 | 3.66e-27 | 114 |
| MsG0880044345.01.T01 | AT5G46100 | 26.577 | 444 | 308 | 8 | 4 | 432 | 11 | 451 | 7.42e-48 | 171 |
| MsG0880044345.01.T01 | AT5G46100 | 29.067 | 375 | 210 | 9 | 113 | 437 | 53 | 421 | 2.27e-34 | 134 |
| MsG0880044345.01.T01 | AT5G46100 | 26.394 | 269 | 186 | 6 | 179 | 440 | 50 | 313 | 1.78e-21 | 97.1 |
| MsG0880044345.01.T01 | AT5G64320 | 26.190 | 504 | 295 | 10 | 7 | 436 | 76 | 576 | 7.91e-48 | 175 |
| MsG0880044345.01.T01 | AT5G64320 | 29.480 | 346 | 235 | 5 | 95 | 436 | 341 | 681 | 2.63e-40 | 154 |
Find 133 sgRNAs with CRISPR-Local
Find 166 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCCATCAAACAGTGTG+CGG | 0.683076 | 8:-40348087 | None:intergenic |
| AAAGGAGGGGTTCAGGGACG+CGG | 0.434897 | 8:-40347232 | None:intergenic |
| AAGACGGGCACGTGCATAGA+AGG | 0.412644 | 8:-40347267 | None:intergenic |
| AAGATTTGAAAAGCGAGAGA+TGG | 0.547380 | 8:-40347080 | None:intergenic |
| AAGCAATGTCCGTCCTGACG+AGG | 0.648208 | 8:+40347535 | MsG0880044345.01.T01:CDS |
| AAGCGAGAGATGGATCCTTT+TGG | 0.338251 | 8:-40347070 | None:intergenic |
| AAGGATGAGATGGAGAGGAA+TGG | 0.505231 | 8:+40347731 | MsG0880044345.01.T01:CDS |
| AAGGCTTTGAAAATTTCAAA+AGG | 0.503784 | 8:-40348346 | None:intergenic |
| AAGGTTTGAATGGCACGTGA+AGG | 0.621344 | 8:-40347290 | None:intergenic |
| AAGTGAGGAGAGCATGGAGG+AGG | 0.495288 | 8:-40347364 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATTGTAACCAAAAACATC+AGG | - | Chr8:40347968-40347987 | None:intergenic | 30.0% | |
| AGAATTTCCAAGAAGCTTAT+AGG | + | Chr8:40347915-40347934 | MsG0880044345.01.T01:CDS | 30.0% | |
| AGTTGAATGATTTGAGAGTA+GGG | - | Chr8:40347343-40347362 | None:intergenic | 30.0% | |
| ATTCATTCAAACTCTTGGAA+TGG | - | Chr8:40348180-40348199 | None:intergenic | 30.0% | |
| GAATAACGATGATGATTTAG+AGG | - | Chr8:40347113-40347132 | None:intergenic | 30.0% | |
| GACAAATTCATTCAAACTCT+TGG | - | Chr8:40348185-40348204 | None:intergenic | 30.0% | |
| GTTTACACTAACTTGATGAA+AGG | + | Chr8:40347668-40347687 | MsG0880044345.01.T01:CDS | 30.0% | |
| TACAAGTAACTGAATTCCAA+TGG | - | Chr8:40347865-40347884 | None:intergenic | 30.0% | |
| TTACACTAACTTGATGAAAG+GGG | + | Chr8:40347670-40347689 | MsG0880044345.01.T01:CDS | 30.0% | |
| TTAGTGTAAACAGAAACACA+TGG | - | Chr8:40347659-40347678 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 40347017 | 40348381 | 40347017 | ID=MsG0880044345.01;Name=MsG0880044345.01 |
| Chr8 | mRNA | 40347017 | 40348381 | 40347017 | ID=MsG0880044345.01.T01;Parent=MsG0880044345.01;Name=MsG0880044345.01.T01;_AED=0.10;_eAED=0.11;_QI=0|-1|0|1|-1|1|1|0|454 |
| Chr8 | exon | 40347017 | 40348381 | 40347017 | ID=MsG0880044345.01.T01:exon:16949;Parent=MsG0880044345.01.T01 |
| Chr8 | CDS | 40347017 | 40348381 | 40347017 | ID=MsG0880044345.01.T01:cds;Parent=MsG0880044345.01.T01 |
| Chr8 | mRNA | 40347179 | 40348381 | 40347179 | ID=MsG0880044345.01.T02;Parent=MsG0880044345.01;Name=MsG0880044345.01.T02;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|400 |
| Chr8 | exon | 40347179 | 40348381 | 40347179 | ID=MsG0880044345.01.T02:exon:16950;Parent=MsG0880044345.01.T02 |
| Chr8 | CDS | 40347179 | 40348381 | 40347179 | ID=MsG0880044345.01.T02:cds;Parent=MsG0880044345.01.T02 |
| Gene Sequence |
>MsG0880044345.01.T02 ATGCTCCCTCAAATGGAACTAATCCTTCAACAACTCCATAACGACACTCGCCACCGCGTCCCTGAACCCCTCCTTTGCCACGTCATCTCCTTCTATGCACGTGCCCGTCTTCCTTCACGTGCCATTCAAACCTTCCTCTCCATCCCTTCCTTCCGCTGCACCCCTACTCTCAAATCATTCAACTCCCTCCTCCATGCTCTCCTCACTTGCCGCCAATTTCAAACCATCACTCACTTCGCATCCCGTTTAAAAGAATTCGGTCCCGCTAATCCGTGCACTTACAACATTCTCATCCGCTCGTGCTTCTTTCAAGGCCGAACTGATCGCGCCTTGGAACTGTTCGATGAAATGCGCAGAAGCAATGTCCGTCCTGACGAGGTCACATTTGGGACGTTAATCCATGGTTTGTGTAAGGATTCGCGGATGCATCAGGCGTTCGGAATGAAAAAATTGATGATCAAGGAATTCAAGCTGAAGCCATGTGTTTCTGTTTACACTAACTTGATGAAAGGGGTTTGTGAGATTGGGGAACTTCATCGGGCTTTTGAGATCAAGGATGAGATGGAGAGGAATGGATTGAGATTGGATGTAGTTGTTTATAACACTCTTGTCAATGCGCTTTTTAAGGCGGGGAGAAAGGAAGAGGCTTTAAGGGTTTTGGAGGAAATGAAAGAGAGTGGTTACCATTGGAATTCAGTTACTTGTAATATAATGATTGGGGAGTTTTGTAGACAAAAGAATTTCCAAGAAGCTTATAGGATTTTGGATGGGGTGGAAGGTGTCAAGCCTGATGTTTTTGGTTACAATGTGGTGATTGGTTGGTTGTGTAAAGAAGGGAAATGGACTGAAGCAAATGATTTGTTTCAGGATATGCCGCGGAGAAAGTGTGTTCCCGATGTTGTAACATACCGCACACTGTTTGATGGGCTTTGTCGGTGGACGCAGTTCCAGGAAGCTGCTATTGTCTTGGATGAGATGTTGTTTAAAGGATATGTTCCCCATTCCAAGAGTTTGAATGAATTTGTCCGTGGGTTGTGTAGGGAAGGTAATTTTGAGTTGTTGTCAACGGTTTTGAGTGGTTTGGCAAGTAGAGGAGAATTCTGTAACGAGGGTATATGGAATGTTTTGGTTTCCATGGTTTGCAAACAGGAAAAGCTGGCAGCAGAACCTTTTGAAATTTTCAAAGCCTTGGCGGTATCATGA >MsG0880044345.01.T01 ATGGGAAGTTCAAAACCTATATCTCCATTCAGACTCTCCTCCCTCCTCCGTTCCCAAAAGGATCCATCTCTCGCTTTTCAAATCTTCCTCAACCCTCTAAATCATCATCGTTATTCCATTCTCTCCTACGACCTCATCATCACCAAACTCGGCCGCGCCAAAATGCTCCCTCAAATGGAACTAATCCTTCAACAACTCCATAACGACACTCGCCACCGCGTCCCTGAACCCCTCCTTTGCCACGTCATCTCCTTCTATGCACGTGCCCGTCTTCCTTCACGTGCCATTCAAACCTTCCTCTCCATCCCTTCCTTCCGCTGCACCCCTACTCTCAAATCATTCAACTCCCTCCTCCATGCTCTCCTCACTTGCCGCCAATTTCAAACCATCACTCACTTCGCATCCCGTTTAAAAGAATTCGGTCCCGCTAATCCGTGCACTTACAACATTCTCATCCGCTCGTGCTTCTTTCAAGGCCGAACTGATCGCGCCTTGGAACTGTTCGATGAAATGCGCAGAAGCAATGTCCGTCCTGACGAGGTCACATTTGGGACGTTAATCCATGGTTTGTGTAAGGATTCGCGGATGCATCAGGCGTTCGGAATGAAAAAATTGATGATCAAGGAATTCAAGCTGAAGCCATGTGTTTCTGTTTACACTAACTTGATGAAAGGGGTTTGTGAGATTGGGGAACTTCATCGGGCTTTTGAGATCAAGGATGAGATGGAGAGGAATGGATTGAGATTGGATGTAGTTGTTTATAACACTCTTGTCAATGCGCTTTTTAAGGCGGGGAGAAAGGAAGAGGCTTTAAGGGTTTTGGAGGAAATGAAAGAGAGTGGTTACCATTGGAATTCAGTTACTTGTAATATAATGATTGGGGAGTTTTGTAGACAAAAGAATTTCCAAGAAGCTTATAGGATTTTGGATGGGGTGGAAGGTGTCAAGCCTGATGTTTTTGGTTACAATGTGGTGATTGGTTGGTTGTGTAAAGAAGGGAAATGGACTGAAGCAAATGATTTGTTTCAGGATATGCCGCGGAGAAAGTGTGTTCCCGATGTTGTAACATACCGCACACTGTTTGATGGGCTTTGTCGGTGGACGCAGTTCCAGGAAGCTGCTATTGTCTTGGATGAGATGTTGTTTAAAGGATATGTTCCCCATTCCAAGAGTTTGAATGAATTTGTCCGTGGGTTGTGTAGGGAAGGTAATTTTGAGTTGTTGTCAACGGTTTTGAGTGGTTTGGCAAGTAGAGGAGAATTCTGTAACGAGGGTATATGGAATGTTTTGGTTTCCATGGTTTGCAAACAGGAAAAGCTGGCAGCAGAACCTTTTGAAATTTTCAAAGCCTTGGCGGTATCATGA |
| Protein sequence |
>MsG0880044345.01.T02 MLPQMELILQQLHNDTRHRVPEPLLCHVISFYARARLPSRAIQTFLSIPSFRCTPTLKSFNSLLHALLTCRQFQTITHFASRLKEFGPANPCTYNILIRSCFFQGRTDRALELFDEMRRSNVRPDEVTFGTLIHGLCKDSRMHQAFGMKKLMIKEFKLKPCVSVYTNLMKGVCEIGELHRAFEIKDEMERNGLRLDVVVYNTLVNALFKAGRKEEALRVLEEMKESGYHWNSVTCNIMIGEFCRQKNFQEAYRILDGVEGVKPDVFGYNVVIGWLCKEGKWTEANDLFQDMPRRKCVPDVVTYRTLFDGLCRWTQFQEAAIVLDEMLFKGYVPHSKSLNEFVRGLCREGNFELLSTVLSGLASRGEFCNEGIWNVLVSMVCKQEKLAAEPFEIFKALAVS* >MsG0880044345.01.T01 MGSSKPISPFRLSSLLRSQKDPSLAFQIFLNPLNHHRYSILSYDLIITKLGRAKMLPQMELILQQLHNDTRHRVPEPLLCHVISFYARARLPSRAIQTFLSIPSFRCTPTLKSFNSLLHALLTCRQFQTITHFASRLKEFGPANPCTYNILIRSCFFQGRTDRALELFDEMRRSNVRPDEVTFGTLIHGLCKDSRMHQAFGMKKLMIKEFKLKPCVSVYTNLMKGVCEIGELHRAFEIKDEMERNGLRLDVVVYNTLVNALFKAGRKEEALRVLEEMKESGYHWNSVTCNIMIGEFCRQKNFQEAYRILDGVEGVKPDVFGYNVVIGWLCKEGKWTEANDLFQDMPRRKCVPDVVTYRTLFDGLCRWTQFQEAAIVLDEMLFKGYVPHSKSLNEFVRGLCREGNFELLSTVLSGLASRGEFCNEGIWNVLVSMVCKQEKLAAEPFEIFKALAVS* |
