Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044358.01.T01 | XP_039685491.1 | 97.656 | 256 | 6 | 0 | 1 | 256 | 1 | 256 | 0 | 525 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044358.01.T01 | Q9LDJ3 | 67.22 | 241 | 79 | 0 | 16 | 256 | 11 | 251 | 7.89E-122 | 350 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044358.01.T01 | A0A1S3EK36 | 88.672 | 256 | 27 | 1 | 1 | 256 | 1 | 254 | 5.00e-169 | 476 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880044358.01 | MsG0180000028.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044358.01.T01 | MTR_7g111010 | 52.381 | 252 | 112 | 3 | 11 | 255 | 4 | 254 | 6.97e-98 | 287 |
| MsG0880044358.01.T01 | MTR_1g085460 | 53.942 | 241 | 105 | 2 | 20 | 255 | 18 | 257 | 2.13e-97 | 286 |
| MsG0880044358.01.T01 | MTR_7g110990 | 52.263 | 243 | 104 | 3 | 18 | 255 | 4 | 239 | 1.54e-94 | 278 |
| MsG0880044358.01.T01 | MTR_1g026020 | 52.227 | 247 | 108 | 4 | 17 | 255 | 11 | 255 | 1.31e-93 | 276 |
| MsG0880044358.01.T01 | MTR_3g435430 | 54.274 | 234 | 97 | 5 | 22 | 250 | 15 | 243 | 1.30e-88 | 263 |
| MsG0880044358.01.T01 | MTR_5g041700 | 56.889 | 225 | 87 | 5 | 31 | 250 | 24 | 243 | 4.70e-87 | 259 |
| MsG0880044358.01.T01 | MTR_4g081950 | 51.181 | 254 | 113 | 6 | 3 | 250 | 31 | 279 | 6.29e-87 | 260 |
| MsG0880044358.01.T01 | MTR_3g094040 | 50.394 | 254 | 114 | 6 | 4 | 250 | 1 | 249 | 1.19e-85 | 256 |
| MsG0880044358.01.T01 | MTR_3g094060 | 51.181 | 254 | 112 | 7 | 4 | 250 | 1 | 249 | 1.51e-85 | 255 |
| MsG0880044358.01.T01 | MTR_7g011060 | 53.975 | 239 | 98 | 6 | 16 | 250 | 6 | 236 | 5.47e-85 | 253 |
| MsG0880044358.01.T01 | MTR_5g075320 | 51.440 | 243 | 105 | 7 | 15 | 249 | 9 | 246 | 6.17e-85 | 254 |
| MsG0880044358.01.T01 | MTR_3g466790 | 50.400 | 250 | 109 | 6 | 10 | 249 | 5 | 249 | 3.93e-83 | 249 |
| MsG0880044358.01.T01 | MTR_5g079950 | 47.451 | 255 | 124 | 5 | 5 | 253 | 6 | 256 | 2.10e-81 | 245 |
| MsG0880044358.01.T01 | MTR_3g466760 | 50.204 | 245 | 107 | 6 | 15 | 249 | 16 | 255 | 2.21e-81 | 245 |
| MsG0880044358.01.T01 | MTR_7g061720 | 50.439 | 228 | 103 | 5 | 32 | 253 | 32 | 255 | 2.66e-80 | 242 |
| MsG0880044358.01.T01 | MTR_2g097030 | 47.561 | 246 | 117 | 6 | 16 | 253 | 16 | 257 | 4.34e-78 | 237 |
| MsG0880044358.01.T01 | MTR_7g013150 | 47.390 | 249 | 120 | 6 | 11 | 255 | 12 | 253 | 1.46e-77 | 235 |
| MsG0880044358.01.T01 | MTR_6g012520 | 55.752 | 226 | 92 | 5 | 31 | 256 | 27 | 244 | 5.41e-76 | 231 |
| MsG0880044358.01.T01 | MTR_4g063975 | 50.000 | 228 | 104 | 5 | 32 | 253 | 32 | 255 | 6.69e-75 | 228 |
| MsG0880044358.01.T01 | MTR_7g013140 | 46.122 | 245 | 123 | 6 | 15 | 255 | 12 | 251 | 1.68e-74 | 227 |
| MsG0880044358.01.T01 | MTR_7g013120 | 46.531 | 245 | 123 | 5 | 15 | 255 | 13 | 253 | 2.65e-74 | 227 |
| MsG0880044358.01.T01 | MTR_8g058990 | 48.361 | 244 | 117 | 6 | 16 | 255 | 15 | 253 | 8.62e-73 | 223 |
| MsG0880044358.01.T01 | MTR_8g464350 | 47.244 | 254 | 119 | 6 | 13 | 255 | 5 | 254 | 2.97e-72 | 221 |
| MsG0880044358.01.T01 | MTR_4g124340 | 45.690 | 232 | 113 | 6 | 29 | 253 | 58 | 283 | 6.28e-71 | 219 |
| MsG0880044358.01.T01 | MTR_4g102450 | 45.525 | 257 | 124 | 7 | 11 | 256 | 11 | 262 | 7.06e-70 | 216 |
| MsG0880044358.01.T01 | MTR_0040s0140 | 45.098 | 255 | 125 | 7 | 1 | 249 | 6 | 251 | 7.06e-69 | 213 |
| MsG0880044358.01.T01 | MTR_5g011210 | 43.077 | 260 | 135 | 7 | 4 | 256 | 10 | 263 | 7.21e-69 | 213 |
| MsG0880044358.01.T01 | MTR_2g090255 | 44.215 | 242 | 125 | 5 | 15 | 250 | 11 | 248 | 3.44e-67 | 209 |
| MsG0880044358.01.T01 | MTR_3g064510 | 46.552 | 232 | 107 | 7 | 32 | 250 | 53 | 280 | 1.24e-66 | 208 |
| MsG0880044358.01.T01 | MTR_5g076950 | 46.121 | 232 | 112 | 5 | 32 | 256 | 41 | 266 | 3.46e-66 | 206 |
| MsG0880044358.01.T01 | MTR_0008s0120 | 34.286 | 245 | 104 | 4 | 11 | 255 | 12 | 199 | 7.97e-39 | 134 |
| MsG0880044358.01.T01 | MTR_2g090270 | 31.418 | 261 | 148 | 10 | 15 | 255 | 9 | 258 | 1.23e-30 | 115 |
| MsG0880044358.01.T01 | MTR_0122s0020 | 35.319 | 235 | 132 | 8 | 32 | 256 | 41 | 265 | 9.32e-30 | 112 |
| MsG0880044358.01.T01 | MTR_2g090265 | 31.660 | 259 | 148 | 10 | 15 | 255 | 9 | 256 | 3.48e-28 | 108 |
| MsG0880044358.01.T01 | MTR_6g012500 | 49.515 | 103 | 51 | 1 | 154 | 256 | 181 | 282 | 7.02e-28 | 108 |
| MsG0880044358.01.T01 | MTR_4g028640 | 32.692 | 260 | 148 | 11 | 9 | 255 | 19 | 264 | 2.55e-25 | 101 |
| MsG0880044358.01.T01 | MTR_0809s0010 | 29.804 | 255 | 153 | 10 | 14 | 255 | 29 | 270 | 5.37e-21 | 89.7 |
| MsG0880044358.01.T01 | MTR_0118s0070 | 30.702 | 228 | 136 | 8 | 37 | 251 | 53 | 271 | 3.71e-20 | 87.4 |
| MsG0880044358.01.T01 | MTR_5g013440 | 29.528 | 254 | 147 | 12 | 15 | 255 | 17 | 251 | 2.59e-18 | 82.0 |
| MsG0880044358.01.T01 | MTR_0122s0020 | 35.632 | 174 | 92 | 8 | 32 | 195 | 41 | 204 | 2.07e-17 | 78.6 |
| MsG0880044358.01.T01 | MTR_4g099370 | 25.000 | 264 | 165 | 11 | 8 | 255 | 4 | 250 | 9.73e-13 | 66.6 |
| MsG0880044358.01.T01 | MTR_1g085365 | 53.704 | 54 | 25 | 0 | 199 | 252 | 4 | 57 | 1.22e-12 | 61.6 |
| MsG0880044358.01.T01 | MTR_4g075790 | 28.511 | 235 | 139 | 10 | 34 | 255 | 25 | 243 | 1.53e-11 | 63.2 |
| MsG0880044358.01.T01 | MTR_4g098930 | 26.336 | 262 | 156 | 10 | 15 | 254 | 5 | 251 | 2.93e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044358.01.T01 | AT3G15370 | 67.220 | 241 | 79 | 0 | 16 | 256 | 11 | 251 | 8.05e-123 | 350 |
| MsG0880044358.01.T01 | AT3G15370 | 60.223 | 269 | 77 | 1 | 16 | 254 | 11 | 279 | 3.82e-116 | 334 |
| MsG0880044358.01.T01 | AT4G01630 | 50.000 | 252 | 120 | 2 | 9 | 255 | 5 | 255 | 5.12e-96 | 282 |
| MsG0880044358.01.T01 | AT1G20190 | 54.393 | 239 | 99 | 4 | 25 | 255 | 16 | 252 | 4.19e-89 | 265 |
| MsG0880044358.01.T01 | AT1G69530 | 54.508 | 244 | 98 | 6 | 15 | 250 | 7 | 245 | 2.67e-88 | 262 |
| MsG0880044358.01.T01 | AT1G69530 | 54.508 | 244 | 98 | 6 | 15 | 250 | 7 | 245 | 2.67e-88 | 262 |
| MsG0880044358.01.T01 | AT1G69530 | 54.508 | 244 | 98 | 6 | 15 | 250 | 7 | 245 | 2.68e-88 | 263 |
| MsG0880044358.01.T01 | AT2G40610 | 51.883 | 239 | 106 | 4 | 16 | 249 | 14 | 248 | 1.55e-87 | 260 |
| MsG0880044358.01.T01 | AT5G56320 | 51.667 | 240 | 106 | 5 | 16 | 250 | 13 | 247 | 1.21e-86 | 258 |
| MsG0880044358.01.T01 | AT5G56320 | 51.667 | 240 | 106 | 5 | 16 | 250 | 13 | 247 | 1.21e-86 | 258 |
| MsG0880044358.01.T01 | AT5G56320 | 51.667 | 240 | 106 | 5 | 16 | 250 | 13 | 247 | 4.28e-86 | 258 |
| MsG0880044358.01.T01 | AT2G03090 | 52.157 | 255 | 105 | 7 | 1 | 250 | 6 | 248 | 4.51e-85 | 254 |
| MsG0880044358.01.T01 | AT2G39700 | 51.012 | 247 | 109 | 6 | 15 | 253 | 12 | 254 | 3.42e-83 | 249 |
| MsG0880044358.01.T01 | AT3G55500 | 47.876 | 259 | 125 | 5 | 1 | 253 | 3 | 257 | 5.70e-83 | 249 |
| MsG0880044358.01.T01 | AT3G29030 | 48.289 | 263 | 109 | 6 | 1 | 250 | 1 | 249 | 1.70e-82 | 248 |
| MsG0880044358.01.T01 | AT5G05290 | 49.174 | 242 | 113 | 5 | 15 | 250 | 14 | 251 | 4.48e-82 | 247 |
| MsG0880044358.01.T01 | AT5G02260 | 51.316 | 228 | 101 | 5 | 32 | 253 | 32 | 255 | 2.56e-81 | 245 |
| MsG0880044358.01.T01 | AT2G37640 | 50.877 | 228 | 102 | 5 | 32 | 253 | 36 | 259 | 4.87e-79 | 239 |
| MsG0880044358.01.T01 | AT1G26770 | 50.622 | 241 | 109 | 5 | 15 | 250 | 9 | 244 | 1.98e-78 | 237 |
| MsG0880044358.01.T01 | AT1G26770 | 50.622 | 241 | 109 | 5 | 15 | 250 | 19 | 254 | 4.81e-78 | 236 |
| MsG0880044358.01.T01 | AT2G28950 | 47.755 | 245 | 116 | 6 | 17 | 253 | 14 | 254 | 6.47e-76 | 231 |
| MsG0880044358.01.T01 | AT5G39280 | 48.899 | 227 | 108 | 5 | 32 | 255 | 54 | 275 | 3.86e-75 | 230 |
| MsG0880044358.01.T01 | AT5G39300 | 48.018 | 227 | 110 | 5 | 32 | 255 | 55 | 276 | 4.16e-75 | 229 |
| MsG0880044358.01.T01 | AT1G20190 | 56.186 | 194 | 78 | 3 | 67 | 255 | 2 | 193 | 4.10e-74 | 224 |
| MsG0880044358.01.T01 | AT1G69530 | 55.340 | 206 | 79 | 6 | 15 | 212 | 7 | 207 | 3.89e-73 | 224 |
| MsG0880044358.01.T01 | AT1G69530 | 55.340 | 206 | 79 | 6 | 15 | 212 | 7 | 207 | 4.60e-73 | 223 |
| MsG0880044358.01.T01 | AT5G39290 | 48.458 | 227 | 109 | 5 | 32 | 255 | 58 | 279 | 1.13e-71 | 221 |
| MsG0880044358.01.T01 | AT2G37640 | 51.185 | 211 | 93 | 5 | 49 | 253 | 28 | 234 | 1.41e-71 | 219 |
| MsG0880044358.01.T01 | AT5G39270 | 48.458 | 227 | 109 | 5 | 32 | 255 | 58 | 279 | 1.91e-71 | 220 |
| MsG0880044358.01.T01 | AT1G12560 | 42.857 | 266 | 136 | 7 | 1 | 255 | 1 | 261 | 7.71e-71 | 218 |
| MsG0880044358.01.T01 | AT3G03220 | 46.903 | 226 | 107 | 5 | 32 | 250 | 40 | 259 | 6.44e-67 | 208 |
| MsG0880044358.01.T01 | AT1G62980 | 41.667 | 252 | 133 | 6 | 14 | 255 | 9 | 256 | 4.06e-65 | 204 |
| MsG0880044358.01.T01 | AT5G39310 | 42.918 | 233 | 124 | 6 | 27 | 255 | 85 | 312 | 3.27e-63 | 201 |
| MsG0880044358.01.T01 | AT4G38210 | 44.578 | 249 | 125 | 7 | 16 | 256 | 12 | 255 | 1.47e-62 | 197 |
| MsG0880044358.01.T01 | AT5G39260 | 44.000 | 225 | 118 | 6 | 32 | 255 | 45 | 262 | 7.27e-61 | 193 |
| MsG0880044358.01.T01 | AT4G28250 | 36.296 | 270 | 141 | 11 | 3 | 255 | 5 | 260 | 4.19e-37 | 132 |
| MsG0880044358.01.T01 | AT2G20750 | 36.929 | 241 | 134 | 9 | 26 | 255 | 34 | 267 | 2.11e-34 | 125 |
| MsG0880044358.01.T01 | AT4G28250 | 34.572 | 269 | 141 | 11 | 3 | 255 | 5 | 254 | 3.25e-32 | 119 |
| MsG0880044358.01.T01 | AT1G65680 | 34.874 | 238 | 130 | 11 | 32 | 255 | 47 | 273 | 7.30e-26 | 102 |
| MsG0880044358.01.T01 | AT2G20750 | 38.418 | 177 | 92 | 8 | 26 | 192 | 34 | 203 | 1.03e-23 | 95.9 |
| MsG0880044358.01.T01 | AT4G28250 | 33.493 | 209 | 105 | 10 | 3 | 196 | 5 | 194 | 1.98e-21 | 89.4 |
| MsG0880044358.01.T01 | AT4G28250 | 34.146 | 205 | 101 | 10 | 3 | 192 | 5 | 190 | 4.01e-21 | 88.6 |
| MsG0880044358.01.T01 | AT3G60570 | 25.941 | 239 | 147 | 8 | 32 | 255 | 34 | 257 | 8.20e-17 | 78.2 |
| MsG0880044358.01.T01 | AT4G17030 | 26.357 | 258 | 152 | 11 | 11 | 252 | 11 | 246 | 2.70e-13 | 68.2 |
| MsG0880044358.01.T01 | AT3G60570 | 25.339 | 221 | 135 | 8 | 50 | 255 | 4 | 209 | 5.31e-13 | 66.6 |
| MsG0880044358.01.T01 | AT3G45960 | 29.000 | 200 | 129 | 7 | 63 | 255 | 6 | 199 | 2.54e-12 | 64.7 |
| MsG0880044358.01.T01 | AT3G45960 | 29.000 | 200 | 129 | 7 | 63 | 255 | 54 | 247 | 3.49e-12 | 65.1 |
| MsG0880044358.01.T01 | AT4G38400 | 26.500 | 200 | 134 | 7 | 63 | 255 | 55 | 248 | 2.17e-11 | 62.8 |
Find 62 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGCCATTGCACAGAAAT+TGG | 0.145280 | 8:-40515057 | MsG0880044358.01.T01:CDS |
| AACGCTCATGCAACGTTCTA+TGG | 0.182142 | 8:-40517402 | MsG0880044358.01.T01:CDS |
| CGACTTCTAAGACACCATTT+TGG | 0.231012 | 8:+40516214 | None:intergenic |
| GAAACTAATAGAATTGATTA+TGG | 0.275627 | 8:+40514900 | None:intergenic |
| TAATCTTGAATGAGAGTCTT+TGG | 0.281520 | 8:+40514999 | None:intergenic |
| ACTATAAAATCGACCCAAAA+TGG | 0.309177 | 8:-40516228 | MsG0880044358.01.T01:CDS |
| TTCAAGATTACTTTGGTTGA+TGG | 0.313364 | 8:-40514986 | MsG0880044358.01.T01:CDS |
| ACAGAAATTGGGGAGCTAAT+TGG | 0.336521 | 8:-40515045 | MsG0880044358.01.T01:CDS |
| CACCATCCTCCATGATTGTT+TGG | 0.340496 | 8:+40516157 | None:intergenic |
| TGGCAACCAAGCTCCACTTC+TGG | 0.354563 | 8:+40515072 | None:intergenic |
| TTGTTCCTGTCCTTTATAGA+AGG | 0.362152 | 8:-40516060 | MsG0880044358.01.T01:intron |
| AAGGCTGGTTGGACTTTGAT+TGG | 0.372747 | 8:+40517377 | None:intergenic |
| GGCAAAGCAGTGCAGATCTA+AGG | 0.383218 | 8:-40515024 | MsG0880044358.01.T01:CDS |
| GTTGCCATTGCACAGAAATT+GGG | 0.387807 | 8:-40515056 | MsG0880044358.01.T01:CDS |
| GTGTCTTCCAATTGGAGATT+TGG | 0.391994 | 8:-40514938 | MsG0880044358.01.T01:CDS |
| GATACCTTTCATGCTGGGTT+TGG | 0.393917 | 8:-40516328 | MsG0880044358.01.T01:CDS |
| GGTGGCAGCGGTGATGTAAA+GGG | 0.404806 | 8:-40515112 | MsG0880044358.01.T01:CDS |
| TGGTGGCAGCGGTGATGTAA+AGG | 0.417985 | 8:-40515113 | MsG0880044358.01.T01:CDS |
| TCAATGTTGTGTCTTCCAAT+TGG | 0.423263 | 8:-40514946 | MsG0880044358.01.T01:CDS |
| GCAAGAGAAGAGGAGGAGTT+AGG | 0.424563 | 8:-40515186 | MsG0880044358.01.T01:CDS |
| TATGATGATACCTTTCATGC+TGG | 0.440717 | 8:-40516334 | MsG0880044358.01.T01:CDS |
| ACTCTCATTCAAGATTACTT+TGG | 0.449267 | 8:-40514993 | MsG0880044358.01.T01:CDS |
| AGGAGTTAGGTTCACATTGA+AGG | 0.456036 | 8:-40515173 | MsG0880044358.01.T01:CDS |
| ACGCTCATGCAACGTTCTAT+GGG | 0.460595 | 8:-40517401 | MsG0880044358.01.T01:CDS |
| ATGATGATACCTTTCATGCT+GGG | 0.461474 | 8:-40516333 | MsG0880044358.01.T01:CDS |
| AAACTAATAGAATTGATTAT+GGG | 0.477188 | 8:+40514901 | None:intergenic |
| TGTTTGTCCAAATCTCCAAT+TGG | 0.490797 | 8:+40514931 | None:intergenic |
| GTCATAACTCATACCAAGGC+TGG | 0.504359 | 8:+40517362 | None:intergenic |
| ATAACAAACGTTGGTGGCAG+CGG | 0.525888 | 8:-40515124 | MsG0880044358.01.T01:CDS |
| GGAGTTAGGTTCACATTGAA+GGG | 0.526521 | 8:-40515172 | MsG0880044358.01.T01:CDS |
| AATCAAAGTCCAACCAGCCT+TGG | 0.527653 | 8:-40517375 | MsG0880044358.01.T01:intron |
| CACACCAAACCCAGCATGAA+AGG | 0.535814 | 8:+40516324 | None:intergenic |
| TTCTTTCGCATTGCACGACA+AGG | 0.541785 | 8:-40516094 | MsG0880044358.01.T01:CDS |
| CGTGCAATGCGAAAGAAAGC+AGG | 0.542098 | 8:+40516100 | None:intergenic |
| AATATTTCAGGAGGAGCTTG+TGG | 0.542340 | 8:-40516358 | MsG0880044358.01.T01:intron |
| GAGGTTCCAGAAGTGGAGCT+TGG | 0.547406 | 8:-40515078 | MsG0880044358.01.T01:CDS |
| GCTCCCCAATTTCTGTGCAA+TGG | 0.548468 | 8:+40515052 | None:intergenic |
| CATCCTCCATGATTGTTTGG+AGG | 0.550332 | 8:+40516160 | None:intergenic |
| GTGGCAGCGGTGATGTAAAG+GGG | 0.556218 | 8:-40515111 | MsG0880044358.01.T01:CDS |
| TGGATAAGAGGTTCCAGAAG+TGG | 0.560366 | 8:-40515085 | MsG0880044358.01.T01:CDS |
| TTGCCATTGCACAGAAATTG+GGG | 0.567784 | 8:-40515055 | MsG0880044358.01.T01:CDS |
| ATTGCACGACAAGGAAATGA+AGG | 0.572173 | 8:-40516085 | MsG0880044358.01.T01:CDS |
| AGAATCCACGTTGAAGCTAA+CGG | 0.575394 | 8:-40517432 | MsG0880044358.01.T01:CDS |
| TAACTCATACCAAGGCTGGT+TGG | 0.575835 | 8:+40517366 | None:intergenic |
| TCCACGTTGAAGCTAACGGT+TGG | 0.587064 | 8:-40517428 | MsG0880044358.01.T01:CDS |
| TTCAGAGATGGTGAGGTGTG+TGG | 0.593383 | 8:-40516274 | MsG0880044358.01.T01:CDS |
| CTCCAAACAATCATGGAGGA+TGG | 0.600779 | 8:-40516159 | MsG0880044358.01.T01:CDS |
| AGGGTAGCATGCAAGAGAAG+AGG | 0.607908 | 8:-40515196 | MsG0880044358.01.T01:CDS |
| TTTGGTTGATGGTAAAACAT+TGG | 0.607982 | 8:-40514975 | MsG0880044358.01.T01:CDS |
| GTAGCATGCAAGAGAAGAGG+AGG | 0.611158 | 8:-40515193 | MsG0880044358.01.T01:CDS |
| GCATGGACATATCAAAGTGA+TGG | 0.615233 | 8:+40516122 | None:intergenic |
| TTAAATGAAGAAATCACAAG+AGG | 0.618476 | 8:+40517495 | None:intergenic |
| GTAATGATAACAAACGTTGG+TGG | 0.620373 | 8:-40515130 | MsG0880044358.01.T01:CDS |
| GACATATCAAAGTGATGGTG+TGG | 0.621785 | 8:+40516127 | None:intergenic |
| ATATCAAAGTGATGGTGTGG+AGG | 0.623230 | 8:+40516130 | None:intergenic |
| ATGGTAATGATAACAAACGT+TGG | 0.631432 | 8:-40515133 | MsG0880044358.01.T01:CDS |
| CGCTCATGCAACGTTCTATG+GGG | 0.631588 | 8:-40517400 | MsG0880044358.01.T01:CDS |
| AATGCGAAAGAAAGCAGGCA+TGG | 0.655376 | 8:+40516105 | None:intergenic |
| GGGTCAATCAAATTTCAACA+TGG | 0.679483 | 8:-40515152 | MsG0880044358.01.T01:CDS |
| TGTCCTCCAAACAATCATGG+AGG | 0.680110 | 8:-40516163 | MsG0880044358.01.T01:CDS |
| GCCAACCGTTAGCTTCAACG+TGG | 0.691827 | 8:+40517427 | None:intergenic |
| TGGCAGCGGTGATGTAAAGG+GGG | 0.713922 | 8:-40515110 | MsG0880044358.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATATGTCTTTAAT+AGG | - | Chr8:40516374-40516393 | MsG0880044358.01.T01:intron | 10.0% |
| !! | CTAAAAAAAGAAATATTTAT+GGG | - | Chr8:40517000-40517019 | MsG0880044358.01.T01:intron | 10.0% |
| !! | TCTAAAAAAAGAAATATTTA+TGG | - | Chr8:40516999-40517018 | MsG0880044358.01.T01:intron | 10.0% |
| !!! | ACAATCTTTTTTTTTAATTA+AGG | - | Chr8:40516710-40516729 | MsG0880044358.01.T01:intron | 10.0% |
| !!! | CAATCTTTTTTTTTAATTAA+GGG | - | Chr8:40516711-40516730 | MsG0880044358.01.T01:intron | 10.0% |
| !!! | TTCAATTTTATCTATAATTA+TGG | - | Chr8:40515135-40515154 | MsG0880044358.01.T01:CDS | 10.0% |
| !!! | TTTCTTCATTTAATTTTATT+TGG | - | Chr8:40514920-40514939 | MsG0880044358.01.T01:CDS | 10.0% |
| !! | AATAATACTTCTCAATAAAA+AGG | + | Chr8:40515459-40515478 | None:intergenic | 15.0% |
| !! | AATAGTAATAAAATTCAACA+TGG | - | Chr8:40515743-40515762 | MsG0880044358.01.T01:intron | 15.0% |
| !! | AATTATATCCCTAAAATAAA+AGG | + | Chr8:40515268-40515287 | None:intergenic | 15.0% |
| !! | AGAAAAAGTTGTAAAAAAAA+TGG | + | Chr8:40515489-40515508 | None:intergenic | 15.0% |
| !! | AGTTAAGATTAGAATATATA+AGG | + | Chr8:40515941-40515960 | None:intergenic | 15.0% |
| !! | ATAAATTTACAAAGAAAAAG+AGG | + | Chr8:40515568-40515587 | None:intergenic | 15.0% |
| !! | ATAATACTTCTCAATAAAAA+GGG | + | Chr8:40515458-40515477 | None:intergenic | 15.0% |
| !! | TGTTATATTAAATTATCAGA+AGG | + | Chr8:40516004-40516023 | None:intergenic | 15.0% |
| !!! | AAGAAATCACTATTTTTTAT+AGG | - | Chr8:40516588-40516607 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | AGAAATCACTATTTTTTATA+GGG | - | Chr8:40516589-40516608 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | ATAATTGTACCTTTTATTTT+AGG | - | Chr8:40515256-40515275 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | TAATTGTACCTTTTATTTTA+GGG | - | Chr8:40515257-40515276 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | TAGTTTTAAATTTATCAAGA+AGG | - | Chr8:40515197-40515216 | MsG0880044358.01.T01:CDS | 15.0% |
| !!! | TCTTAAATTTTTGATTGTAT+AGG | - | Chr8:40516521-40516540 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | TCTTTTTCTTTGTAAATTTA+TGG | - | Chr8:40515567-40515586 | MsG0880044358.01.T01:intron | 15.0% |
| !!! | TGGTCTATATAAAATATTTT+TGG | + | Chr8:40515853-40515872 | None:intergenic | 15.0% |
| !!! | TTTTATTTTACCTTCTATAA+AGG | + | Chr8:40516358-40516377 | None:intergenic | 15.0% |
| !! | AAAAAAGAAATATTTATGGG+TGG | - | Chr8:40517003-40517022 | MsG0880044358.01.T01:intron | 20.0% |
| !! | AATACCTGTTAAATACTTAA+GGG | - | Chr8:40516742-40516761 | MsG0880044358.01.T01:intron | 20.0% |
| !! | ATTTATGTAAAGATTTAGAG+AGG | + | Chr8:40515876-40515895 | None:intergenic | 20.0% |
| !! | GGTTCTTAATATTAAACAAA+TGG | + | Chr8:40515983-40516002 | None:intergenic | 20.0% |
| !! | TAATACTTCTCAATAAAAAG+GGG | + | Chr8:40515457-40515476 | None:intergenic | 20.0% |
| !! | TTAGTCCATAATTATAATGT+AGG | - | Chr8:40517160-40517179 | MsG0880044358.01.T01:intron | 20.0% |
| !! | TTGTTAAACAATGTCAATAT+TGG | + | Chr8:40516550-40516569 | None:intergenic | 20.0% |
| !! | TTGTTTAAGTACTTTAAGTA+TGG | + | Chr8:40516694-40516713 | None:intergenic | 20.0% |
| !!! | AAAACTTTAAATCTCTTTTG+AGG | + | Chr8:40516977-40516996 | None:intergenic | 20.0% |
| !!! | AATAATTTTACACGCATATA+AGG | + | Chr8:40515294-40515313 | None:intergenic | 20.0% |
| !!! | TATTTGGTGTCTTTTATTAT+TGG | - | Chr8:40514936-40514955 | MsG0880044358.01.T01:CDS | 20.0% |
| !!! | TTTTACGAATGCATATAATA+TGG | - | Chr8:40516950-40516969 | MsG0880044358.01.T01:intron | 20.0% |
| ! | AAAATCCACTATTTCACTAA+TGG | - | Chr8:40517089-40517108 | MsG0880044358.01.T01:intron | 25.0% |
| ! | CAATACCTGTTAAATACTTA+AGG | - | Chr8:40516741-40516760 | MsG0880044358.01.T01:intron | 25.0% |
| ! | TGTGATTATGACAATATTTC+AGG | - | Chr8:40516035-40516054 | MsG0880044358.01.T01:intron | 25.0% |
| ! | TTAAATGAAGAAATCACAAG+AGG | + | Chr8:40514913-40514932 | None:intergenic | 25.0% |
| ! | TTTAACAAAAAACCAACAAC+TGG | - | Chr8:40516562-40516581 | MsG0880044358.01.T01:intron | 25.0% |
| !! | ATCTACTTGTAAGTTTTACA+TGG | - | Chr8:40515825-40515844 | MsG0880044358.01.T01:intron | 25.0% |
| !! | TTTTATAGTCACATATGACT+TGG | + | Chr8:40516166-40516185 | None:intergenic | 25.0% |
| ACTATAAAATCGACCCAAAA+TGG | - | Chr8:40516177-40516196 | MsG0880044358.01.T01:CDS | 30.0% | |
| ACTCTCATTCAAGATTACTT+TGG | - | Chr8:40517412-40517431 | MsG0880044358.01.T01:CDS | 30.0% | |
| ATATGTCATAACTCATACCA+AGG | + | Chr8:40515050-40515069 | None:intergenic | 30.0% | |
| ATGTCTTTAATAGGCAATGA+TGG | - | Chr8:40516383-40516402 | MsG0880044358.01.T01:intron | 30.0% | |
| CTGATCATGTATCGTATAAA+GGG | + | Chr8:40516830-40516849 | None:intergenic | 30.0% | |
| CTGTTCAAGTAAGTACAATA+CGG | + | Chr8:40517191-40517210 | None:intergenic | 30.0% | |
| CTTTATACGATACATGATCA+GGG | - | Chr8:40516829-40516848 | MsG0880044358.01.T01:intron | 30.0% | |
| GATAACCATTAGTGAAATAG+TGG | + | Chr8:40517097-40517116 | None:intergenic | 30.0% | |
| GATTATGACAATATTTCAGG+AGG | - | Chr8:40516038-40516057 | MsG0880044358.01.T01:intron | 30.0% | |
| GTATTGTACTTACTTGAACA+GGG | - | Chr8:40517190-40517209 | MsG0880044358.01.T01:intron | 30.0% | |
| TAATCTTGAATGAGAGTCTT+TGG | + | Chr8:40517409-40517428 | None:intergenic | 30.0% | |
| TATGTAAAGATTTAGAGAGG+TGG | + | Chr8:40515873-40515892 | None:intergenic | 30.0% | |
| TGAGGCCTACATTATAATTA+TGG | + | Chr8:40517168-40517187 | None:intergenic | 30.0% | |
| TGATTTCTTTATCCAGTTGT+TGG | + | Chr8:40516577-40516596 | None:intergenic | 30.0% | |
| TTCAAGATTACTTTGGTTGA+TGG | - | Chr8:40517419-40517438 | MsG0880044358.01.T01:CDS | 30.0% | |
| ! | AAAACAAAAGCACATGATGA+GGG | - | Chr8:40516858-40516877 | MsG0880044358.01.T01:intron | 30.0% |
| ! | ATGGTAATGATAACAAACGT+TGG | - | Chr8:40517272-40517291 | MsG0880044358.01.T01:intron | 30.0% |
| ! | GTATTTAACAGGTATTGCTT+TGG | + | Chr8:40516738-40516757 | None:intergenic | 30.0% |
| ! | TTAAGGCTTAAATGCTCTTT+TGG | + | Chr8:40516769-40516788 | None:intergenic | 30.0% |
| ! | TTTTACCTTCTATAAAGGAC+AGG | + | Chr8:40516353-40516372 | None:intergenic | 30.0% |
| !! | TTTGGTTGATGGTAAAACAT+TGG | - | Chr8:40517430-40517449 | MsG0880044358.01.T01:CDS | 30.0% |
| AACGAAAAAGTTTACGTGTG+TGG | + | Chr8:40517142-40517161 | None:intergenic | 35.0% | |
| ACTACTCTCTTACTACTAGT+TGG | - | Chr8:40516914-40516933 | MsG0880044358.01.T01:intron | 35.0% | |
| ATGATGATACCTTTCATGCT+GGG | - | Chr8:40516072-40516091 | MsG0880044358.01.T01:CDS | 35.0% | |
| CCTGATCATGTATCGTATAA+AGG | + | Chr8:40516831-40516850 | None:intergenic | 35.0% | |
| CCTTTATACGATACATGATC+AGG | - | Chr8:40516828-40516847 | MsG0880044358.01.T01:intron | 35.0% | |
| CGTATTGTACTTACTTGAAC+AGG | - | Chr8:40517189-40517208 | MsG0880044358.01.T01:intron | 35.0% | |
| GGGTCAATCAAATTTCAACA+TGG | - | Chr8:40517253-40517272 | MsG0880044358.01.T01:intron | 35.0% | |
| GTAATGATAACAAACGTTGG+TGG | - | Chr8:40517275-40517294 | MsG0880044358.01.T01:intron | 35.0% | |
| GTTTGGAGGACAAAAATTTG+TGG | + | Chr8:40516234-40516253 | None:intergenic | 35.0% | |
| TATGATGATACCTTTCATGC+TGG | - | Chr8:40516071-40516090 | MsG0880044358.01.T01:CDS | 35.0% | |
| TCAATGTTGTGTCTTCCAAT+TGG | - | Chr8:40517459-40517478 | MsG0880044358.01.T01:CDS | 35.0% | |
| TGGTCCCTTAAGTATTTAAC+AGG | + | Chr8:40516749-40516768 | None:intergenic | 35.0% | |
| TGTTTGTCCAAATCTCCAAT+TGG | + | Chr8:40517477-40517496 | None:intergenic | 35.0% | |
| TTGTTCCTGTCCTTTATAGA+AGG | - | Chr8:40516345-40516364 | MsG0880044358.01.T01:CDS | 35.0% | |
| ! | ATGGGTGGACTTTACTATTA+TGG | - | Chr8:40517018-40517037 | MsG0880044358.01.T01:intron | 35.0% |
| ! | CAAAACAAAAGCACATGATG+AGG | - | Chr8:40516857-40516876 | MsG0880044358.01.T01:intron | 35.0% |
| ! | CAAGTTAACTAGTGCTTTTG+AGG | - | Chr8:40516630-40516649 | MsG0880044358.01.T01:intron | 35.0% |
| ! | TTTTGTCCTCCAAACAATCA+TGG | - | Chr8:40516239-40516258 | MsG0880044358.01.T01:CDS | 35.0% |
| !! | CAAAAGCACTAGTTAACTTG+AGG | + | Chr8:40516630-40516649 | None:intergenic | 35.0% |
| !!! | CACACGCACACTTTATTTTA+AGG | + | Chr8:40516786-40516805 | None:intergenic | 35.0% |
| !!! | GACTTCTAAGACACCATTTT+GGG | + | Chr8:40516193-40516212 | None:intergenic | 35.0% |
| AATATTTCAGGAGGAGCTTG+TGG | - | Chr8:40516047-40516066 | MsG0880044358.01.T01:intron | 40.0% | |
| ACAGAAATTGGGGAGCTAAT+TGG | - | Chr8:40517360-40517379 | MsG0880044358.01.T01:intron | 40.0% | |
| AGAATCCACGTTGAAGCTAA+CGG | - | Chr8:40514973-40514992 | MsG0880044358.01.T01:CDS | 40.0% | |
| AGGAGTTAGGTTCACATTGA+AGG | - | Chr8:40517232-40517251 | MsG0880044358.01.T01:intron | 40.0% | |
| ATCACAAAGCTCCTGCTATA+GGG | - | Chr8:40517114-40517133 | MsG0880044358.01.T01:intron | 40.0% | |
| ATTGCACGACAAGGAAATGA+AGG | - | Chr8:40516320-40516339 | MsG0880044358.01.T01:CDS | 40.0% | |
| CAAGTAAGTACAATACGGTG+AGG | + | Chr8:40517186-40517205 | None:intergenic | 40.0% | |
| GCATGGACATATCAAAGTGA+TGG | + | Chr8:40516286-40516305 | None:intergenic | 40.0% | |
| GGAGTTAGGTTCACATTGAA+GGG | - | Chr8:40517233-40517252 | MsG0880044358.01.T01:intron | 40.0% | |
| GTGTCTTCCAATTGGAGATT+TGG | - | Chr8:40517467-40517486 | MsG0880044358.01.T01:CDS | 40.0% | |
| GTTGCCATTGCACAGAAATT+GGG | - | Chr8:40517349-40517368 | MsG0880044358.01.T01:intron | 40.0% | |
| TATCACAAAGCTCCTGCTAT+AGG | - | Chr8:40517113-40517132 | MsG0880044358.01.T01:intron | 40.0% | |
| TTGCCATTGCACAGAAATTG+GGG | - | Chr8:40517350-40517369 | MsG0880044358.01.T01:intron | 40.0% | |
| ! | AGCACTATGCTTTTCAGAGA+TGG | - | Chr8:40516119-40516138 | MsG0880044358.01.T01:CDS | 40.0% |
| ! | ATATCAAAGTGATGGTGTGG+AGG | + | Chr8:40516278-40516297 | None:intergenic | 40.0% |
| ! | GACATATCAAAGTGATGGTG+TGG | + | Chr8:40516281-40516300 | None:intergenic | 40.0% |
| ! | TATGCTTTTCAGAGATGGTG+AGG | - | Chr8:40516124-40516143 | MsG0880044358.01.T01:CDS | 40.0% |
| !!! | CGACTTCTAAGACACCATTT+TGG | + | Chr8:40516194-40516213 | None:intergenic | 40.0% |
| AACGCTCATGCAACGTTCTA+TGG | - | Chr8:40515003-40515022 | MsG0880044358.01.T01:CDS | 45.0% | |
| AATCAAAGTCCAACCAGCCT+TGG | - | Chr8:40515030-40515049 | MsG0880044358.01.T01:CDS | 45.0% | |
| AATGCGAAAGAAAGCAGGCA+TGG | + | Chr8:40516303-40516322 | None:intergenic | 45.0% | |
| ACGCTCATGCAACGTTCTAT+GGG | - | Chr8:40515004-40515023 | MsG0880044358.01.T01:CDS | 45.0% | |
| CACCATCCTCCATGATTGTT+TGG | + | Chr8:40516251-40516270 | None:intergenic | 45.0% | |
| CATCCTCCATGATTGTTTGG+AGG | + | Chr8:40516248-40516267 | None:intergenic | 45.0% | |
| CTCCAAACAATCATGGAGGA+TGG | - | Chr8:40516246-40516265 | MsG0880044358.01.T01:CDS | 45.0% | |
| GATACCTTTCATGCTGGGTT+TGG | - | Chr8:40516077-40516096 | MsG0880044358.01.T01:CDS | 45.0% | |
| GGTTGCCATTGCACAGAAAT+TGG | - | Chr8:40517348-40517367 | MsG0880044358.01.T01:intron | 45.0% | |
| GTAGTGCTCATACAGAGATC+TGG | + | Chr8:40516899-40516918 | None:intergenic | 45.0% | |
| GTCATAACTCATACCAAGGC+TGG | + | Chr8:40515046-40515065 | None:intergenic | 45.0% | |
| TAACTCATACCAAGGCTGGT+TGG | + | Chr8:40515042-40515061 | None:intergenic | 45.0% | |
| TGGATAAGAGGTTCCAGAAG+TGG | - | Chr8:40517320-40517339 | MsG0880044358.01.T01:intron | 45.0% | |
| TGTCCTCCAAACAATCATGG+AGG | - | Chr8:40516242-40516261 | MsG0880044358.01.T01:CDS | 45.0% | |
| TTCTTTCGCATTGCACGACA+AGG | - | Chr8:40516311-40516330 | MsG0880044358.01.T01:CDS | 45.0% | |
| ! | AAGGCTGGTTGGACTTTGAT+TGG | + | Chr8:40515031-40515050 | None:intergenic | 45.0% |
| !! | ATAACAAACGTTGGTGGCAG+CGG | - | Chr8:40517281-40517300 | MsG0880044358.01.T01:intron | 45.0% |
| !! | TAAAATTAAATAATTAACTT+TGG | - | Chr8:40515785-40515804 | MsG0880044358.01.T01:intron | 5.0% |
| !!! | TTTTTTATCTATAATTTTAT+TGG | - | Chr8:40515109-40515128 | MsG0880044358.01.T01:CDS | 5.0% |
| AGGGTAGCATGCAAGAGAAG+AGG | - | Chr8:40517209-40517228 | MsG0880044358.01.T01:intron | 50.0% | |
| CACACCAAACCCAGCATGAA+AGG | + | Chr8:40516084-40516103 | None:intergenic | 50.0% | |
| CGCTCATGCAACGTTCTATG+GGG | - | Chr8:40515005-40515024 | MsG0880044358.01.T01:CDS | 50.0% | |
| CGTGCAATGCGAAAGAAAGC+AGG | + | Chr8:40516308-40516327 | None:intergenic | 50.0% | |
| GCAAGAGAAGAGGAGGAGTT+AGG | - | Chr8:40517219-40517238 | MsG0880044358.01.T01:intron | 50.0% | |
| GCTCCCCAATTTCTGTGCAA+TGG | + | Chr8:40517356-40517375 | None:intergenic | 50.0% | |
| GGCAAAGCAGTGCAGATCTA+AGG | - | Chr8:40517381-40517400 | MsG0880044358.01.T01:CDS | 50.0% | |
| GTAGCATGCAAGAGAAGAGG+AGG | - | Chr8:40517212-40517231 | MsG0880044358.01.T01:intron | 50.0% | |
| TCCACGTTGAAGCTAACGGT+TGG | - | Chr8:40514977-40514996 | MsG0880044358.01.T01:CDS | 50.0% | |
| TTCAGAGATGGTGAGGTGTG+TGG | - | Chr8:40516131-40516150 | MsG0880044358.01.T01:CDS | 50.0% | |
| ! | GTAAAGGGGGTGTGGATAAG+AGG | - | Chr8:40517308-40517327 | MsG0880044358.01.T01:intron | 50.0% |
| CGTGTGTGGATCCCTATAGC+AGG | + | Chr8:40517128-40517147 | None:intergenic | 55.0% | |
| GAGGTTCCAGAAGTGGAGCT+TGG | - | Chr8:40517327-40517346 | MsG0880044358.01.T01:intron | 55.0% | |
| GGTGGCAGCGGTGATGTAAA+GGG | - | Chr8:40517293-40517312 | MsG0880044358.01.T01:intron | 55.0% | |
| GTGGCAGCGGTGATGTAAAG+GGG | - | Chr8:40517294-40517313 | MsG0880044358.01.T01:intron | 55.0% | |
| TGGCAACCAAGCTCCACTTC+TGG | + | Chr8:40517336-40517355 | None:intergenic | 55.0% | |
| TGGCAGCGGTGATGTAAAGG+GGG | - | Chr8:40517295-40517314 | MsG0880044358.01.T01:intron | 55.0% | |
| TGGTGGCAGCGGTGATGTAA+AGG | - | Chr8:40517292-40517311 | MsG0880044358.01.T01:intron | 55.0% | |
| ! | GCCAACCGTTAGCTTCAACG+TGG | + | Chr8:40514981-40515000 | None:intergenic | 55.0% |
| GCGGTGATGTAAAGGGGGTG+TGG | - | Chr8:40517300-40517319 | MsG0880044358.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 40514904 | 40517523 | 40514904 | ID=MsG0880044358.01;Name=MsG0880044358.01 |
| Chr8 | mRNA | 40514904 | 40517523 | 40514904 | ID=MsG0880044358.01.T01;Parent=MsG0880044358.01;Name=MsG0880044358.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|256 |
| Chr8 | exon | 40517376 | 40517523 | 40517376 | ID=MsG0880044358.01.T01:exon:7803;Parent=MsG0880044358.01.T01 |
| Chr8 | exon | 40516061 | 40516370 | 40516061 | ID=MsG0880044358.01.T01:exon:7802;Parent=MsG0880044358.01.T01 |
| Chr8 | exon | 40514904 | 40515216 | 40514904 | ID=MsG0880044358.01.T01:exon:7801;Parent=MsG0880044358.01.T01 |
| Chr8 | CDS | 40517376 | 40517523 | 40517376 | ID=MsG0880044358.01.T01:cds;Parent=MsG0880044358.01.T01 |
| Chr8 | CDS | 40516061 | 40516370 | 40516061 | ID=MsG0880044358.01.T01:cds;Parent=MsG0880044358.01.T01 |
| Chr8 | CDS | 40514904 | 40515216 | 40514904 | ID=MsG0880044358.01.T01:cds;Parent=MsG0880044358.01.T01 |
| Gene Sequence |
| Protein sequence |
