Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044578.01.T01 | AFK35337.1 | 91.781 | 73 | 6 | 0 | 1 | 73 | 306 | 378 | 3.82E-36 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044578.01.T01 | A7M6E8 | 43.59 | 78 | 35 | 2 | 1 | 73 | 404 | 477 | 5.16E-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044578.01.T01 | I3S4Z5 | 91.781 | 73 | 6 | 0 | 1 | 73 | 306 | 378 | 1.82e-36 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880044577.01 | MsG0880044578.01 | 0.953246 | 4.181399e-111 | 1.084146e-105 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044578.01.T01 | MTR_8g076950 | 91.781 | 73 | 6 | 0 | 1 | 73 | 404 | 476 | 6.37e-40 | 138 |
| MsG0880044578.01.T01 | MTR_8g076950 | 91.781 | 73 | 6 | 0 | 1 | 73 | 404 | 476 | 8.79e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAACACTCATGAACATTT+TGG | 0.213611 | 8:-44924216 | None:intergenic |
| ACTAGTTGATGCATATTTAG+AGG | 0.320863 | 8:+44924135 | MsG0880044578.01.T01:CDS |
| ATTCAAGGTCATTAATATGA+TGG | 0.449625 | 8:-44924102 | None:intergenic |
| TTCAAGGTCATTAATATGAT+GGG | 0.458039 | 8:-44924101 | None:intergenic |
| TTTGCATCTGGGAATTCGCT+AGG | 0.490206 | 8:-44924277 | None:intergenic |
| CTAGTGTGTTCGCAAATTCA+AGG | 0.538039 | 8:-44924117 | None:intergenic |
| ATCTAAATGAAATGATAACC+TGG | 0.550382 | 8:-44924301 | None:intergenic |
| GAATTCCCAGATGCAAAACC+AGG | 0.557028 | 8:+44924283 | MsG0880044578.01.T01:CDS |
| TCAAGGTCATTAATATGATG+GGG | 0.645837 | 8:-44924100 | None:intergenic |
| GTTGTGTATTGTGCAGGTGA+AGG | 0.646194 | 8:+44924072 | None:intergenic |
| TTCGCTAGGTGCATAGACAA+TGG | 0.657056 | 8:-44924263 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCAAGGTCATTAATATGA+TGG | - | Chr8:44924105-44924124 | None:intergenic | 25.0% |
| ! | TTCAAGGTCATTAATATGAT+GGG | - | Chr8:44924104-44924123 | None:intergenic | 25.0% |
| ! | TTTCGAAAAAATCAAGCTTT+GGG | + | Chr8:44924239-44924258 | MsG0880044578.01.T01:CDS | 25.0% |
| !! | GTAGAATCTTTAACAGTTTT+CGG | - | Chr8:44924166-44924185 | None:intergenic | 25.0% |
| GTTTCGAAAAAATCAAGCTT+TGG | + | Chr8:44924238-44924257 | MsG0880044578.01.T01:CDS | 30.0% | |
| TCAAGGTCATTAATATGATG+GGG | - | Chr8:44924103-44924122 | None:intergenic | 30.0% | |
| ! | ACTAGTTGATGCATATTTAG+AGG | + | Chr8:44924135-44924154 | MsG0880044578.01.T01:CDS | 30.0% |
| ! | ATTTTGGACAGTTTCAGGAA+TGG | - | Chr8:44924203-44924222 | None:intergenic | 35.0% |
| ! | CAGAACACTCATGAACATTT+TGG | - | Chr8:44924219-44924238 | None:intergenic | 35.0% |
| ! | TGAACATTTTGGACAGTTTC+AGG | - | Chr8:44924208-44924227 | None:intergenic | 35.0% |
| CTAGTGTGTTCGCAAATTCA+AGG | - | Chr8:44924120-44924139 | None:intergenic | 40.0% | |
| !!! | GATAACCTGGTTTTGCATCT+GGG | - | Chr8:44924291-44924310 | None:intergenic | 40.0% |
| !!! | TGATAACCTGGTTTTGCATC+TGG | - | Chr8:44924292-44924311 | None:intergenic | 40.0% |
| GAATTCCCAGATGCAAAACC+AGG | + | Chr8:44924283-44924302 | MsG0880044578.01.T01:CDS | 45.0% | |
| TTCGCTAGGTGCATAGACAA+TGG | - | Chr8:44924266-44924285 | None:intergenic | 45.0% | |
| TTTGCATCTGGGAATTCGCT+AGG | - | Chr8:44924280-44924299 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 44924085 | 44924321 | 44924085 | ID=MsG0880044578.01;Name=MsG0880044578.01 |
| Chr8 | mRNA | 44924085 | 44924321 | 44924085 | ID=MsG0880044578.01.T01;Parent=MsG0880044578.01;Name=MsG0880044578.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|78 |
| Chr8 | exon | 44924085 | 44924321 | 44924085 | ID=MsG0880044578.01.T01:exon:11905;Parent=MsG0880044578.01.T01 |
| Chr8 | CDS | 44924085 | 44924321 | 44924085 | ID=MsG0880044578.01.T01:cds;Parent=MsG0880044578.01.T01 |
| Gene Sequence |
| Protein sequence |
