Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044577.01.T01 | AFK35337.1 | 91.781 | 73 | 6 | 0 | 1 | 73 | 306 | 378 | 3.82E-36 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044577.01.T01 | A7M6E8 | 43.59 | 78 | 35 | 2 | 1 | 73 | 404 | 477 | 5.16E-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044577.01.T01 | I3S4Z5 | 91.781 | 73 | 6 | 0 | 1 | 73 | 306 | 378 | 1.82e-36 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880044577.01 | MsG0880044578.01 | 0.953246 | 4.181399e-111 | 1.084146e-105 |
| MsG0180005974.01 | MsG0880044577.01 | 0.811971 | 5.361147e-51 | 3.365879e-48 |
| MsG0380013596.01 | MsG0880044577.01 | 0.800194 | 1.616152e-48 | 7.478600e-46 |
| MsG0580025638.01 | MsG0880044577.01 | 0.839376 | 1.644180e-57 | 2.266372e-54 |
| MsG0380017822.01 | MsG0880044577.01 | 0.800314 | 1.527761e-48 | 7.091578e-46 |
| MsG0580027926.01 | MsG0880044577.01 | 0.804515 | 2.082075e-49 | 1.075592e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044577.01.T01 | MTR_8g076950 | 91.781 | 73 | 6 | 0 | 1 | 73 | 404 | 476 | 6.37e-40 | 138 |
| MsG0880044577.01.T01 | MTR_8g076950 | 91.781 | 73 | 6 | 0 | 1 | 73 | 404 | 476 | 8.79e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAACACTCATGAACATTT+TGG | 0.213611 | 8:-44908708 | None:intergenic |
| ACTAGTTGATGCATATTTAG+AGG | 0.320863 | 8:+44908627 | MsG0880044577.01.T01:CDS |
| ATTCAAGGTCATTAATATGA+TGG | 0.449625 | 8:-44908594 | None:intergenic |
| TTCAAGGTCATTAATATGAT+GGG | 0.458039 | 8:-44908593 | None:intergenic |
| TTTGCATCTGGGAATTCGCT+AGG | 0.490206 | 8:-44908769 | None:intergenic |
| CTAGTGTGTTCGCAAATTCA+AGG | 0.538039 | 8:-44908609 | None:intergenic |
| ATCTAAATGAAATGATAACC+TGG | 0.550382 | 8:-44908793 | None:intergenic |
| GAATTCCCAGATGCAAAACC+AGG | 0.557028 | 8:+44908775 | MsG0880044577.01.T01:CDS |
| TCAAGGTCATTAATATGATG+GGG | 0.645837 | 8:-44908592 | None:intergenic |
| GTTGTGTATTGTGCAGGTGA+AGG | 0.646194 | 8:+44908564 | None:intergenic |
| TTCGCTAGGTGCATAGACAA+TGG | 0.657056 | 8:-44908755 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCAAGGTCATTAATATGA+TGG | - | Chr8:44908597-44908616 | None:intergenic | 25.0% |
| ! | TTCAAGGTCATTAATATGAT+GGG | - | Chr8:44908596-44908615 | None:intergenic | 25.0% |
| ! | TTTCGAAAAAATCAAGCTTT+GGG | + | Chr8:44908731-44908750 | MsG0880044577.01.T01:CDS | 25.0% |
| !! | GTAGAATCTTTAACAGTTTT+CGG | - | Chr8:44908658-44908677 | None:intergenic | 25.0% |
| GTTTCGAAAAAATCAAGCTT+TGG | + | Chr8:44908730-44908749 | MsG0880044577.01.T01:CDS | 30.0% | |
| TCAAGGTCATTAATATGATG+GGG | - | Chr8:44908595-44908614 | None:intergenic | 30.0% | |
| ! | ACTAGTTGATGCATATTTAG+AGG | + | Chr8:44908627-44908646 | MsG0880044577.01.T01:CDS | 30.0% |
| ! | ATTTTGGACAGTTTCAGGAA+TGG | - | Chr8:44908695-44908714 | None:intergenic | 35.0% |
| ! | CAGAACACTCATGAACATTT+TGG | - | Chr8:44908711-44908730 | None:intergenic | 35.0% |
| ! | TGAACATTTTGGACAGTTTC+AGG | - | Chr8:44908700-44908719 | None:intergenic | 35.0% |
| CTAGTGTGTTCGCAAATTCA+AGG | - | Chr8:44908612-44908631 | None:intergenic | 40.0% | |
| !!! | GATAACCTGGTTTTGCATCT+GGG | - | Chr8:44908783-44908802 | None:intergenic | 40.0% |
| !!! | TGATAACCTGGTTTTGCATC+TGG | - | Chr8:44908784-44908803 | None:intergenic | 40.0% |
| GAATTCCCAGATGCAAAACC+AGG | + | Chr8:44908775-44908794 | MsG0880044577.01.T01:CDS | 45.0% | |
| TTCGCTAGGTGCATAGACAA+TGG | - | Chr8:44908758-44908777 | None:intergenic | 45.0% | |
| TTTGCATCTGGGAATTCGCT+AGG | - | Chr8:44908772-44908791 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 44908577 | 44908813 | 44908577 | ID=MsG0880044577.01;Name=MsG0880044577.01 |
| Chr8 | mRNA | 44908577 | 44908813 | 44908577 | ID=MsG0880044577.01.T01;Parent=MsG0880044577.01;Name=MsG0880044577.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|78 |
| Chr8 | exon | 44908577 | 44908813 | 44908577 | ID=MsG0880044577.01.T01:exon:11904;Parent=MsG0880044577.01.T01 |
| Chr8 | CDS | 44908577 | 44908813 | 44908577 | ID=MsG0880044577.01.T01:cds;Parent=MsG0880044577.01.T01 |
| Gene Sequence |
| Protein sequence |
