AlfalfaGEDB | MsG0880044793.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044793.01

KEGG: K09422
GO: GO:0003677
PFAM: PF13921

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044793.01.T01	XP_013445757.1	94.75	400	21	0	1	400	1	400	0	788

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044793.01.T01	Q94FL6	44.471	416	172	16	13	400	16	400	5.30E-97	300

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044793.01.T01	A0A072TSE8	94.750	400	21	0	1	400	1	400	0.0	788

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0780040887.01	MsG0880044793.01	PPI
MsG0780041656.01	MsG0880044793.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044793.01.T01	MTR_8g468380	94.750	400	21	0	1	400	1	400	0.0	788
MsG0880044793.01.T01	MTR_5g038910	55.023	438	147	15	1	400	6	431	1.65e-144	419
MsG0880044793.01.T01	MTR_5g038910	55.612	392	131	13	1	361	6	385	2.47e-128	375
MsG0880044793.01.T01	MTR_7g061330	47.959	98	50	1	25	121	116	213	7.73e-25	107
MsG0880044793.01.T01	MTR_7g461410	47.959	98	50	1	25	121	116	213	7.73e-25	107
MsG0880044793.01.T01	MTR_7g061330	47.959	98	50	1	25	121	80	177	1.15e-24	106
MsG0880044793.01.T01	MTR_1g026870	40.816	98	58	0	25	122	88	185	3.95e-23	102
MsG0880044793.01.T01	MTR_3g110028	39.796	98	59	0	25	122	88	185	1.85e-22	100
MsG0880044793.01.T01	MTR_7g035350	33.333	147	96	1	25	169	228	374	6.96e-22	97.4
MsG0880044793.01.T01	MTR_7g451170	38.760	129	71	2	4	124	172	300	7.89e-22	97.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044793.01.T01	AT1G14350	44.471	416	172	16	13	400	16	400	5.40e-98	300
MsG0880044793.01.T01	AT1G14350	44.471	416	172	16	13	400	16	400	5.40e-98	300
MsG0880044793.01.T01	AT2G02820	43.478	437	193	15	15	404	23	452	7.55e-97	297
MsG0880044793.01.T01	AT2G02820	43.187	433	192	15	15	400	23	448	1.05e-93	290
MsG0880044793.01.T01	AT2G02820	46.326	313	131	8	13	289	21	332	1.19e-81	254
MsG0880044793.01.T01	AT1G14350	39.344	366	163	16	63	400	23	357	1.68e-65	214
MsG0880044793.01.T01	AT1G14350	39.344	366	163	16	63	400	23	357	1.68e-65	214
MsG0880044793.01.T01	AT3G27785	38.760	129	65	2	25	139	192	320	1.71e-24	105
MsG0880044793.01.T01	AT5G40360	41.463	123	61	2	25	140	161	279	1.75e-24	103
MsG0880044793.01.T01	AT5G02320	44.898	98	53	1	25	121	130	227	2.67e-23	102

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Find 84 sgRNAs with CRISPR-Local

Find 340 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGGAGTCACTTGAAG+AGG	0.573906	8:-49291308	None:intergenic
AAACCAGACAAGGACTCCCC+AGG	0.613720	8:+49290462	MsG0880044793.01.T01:CDS
AAATAGGAGTCACTTGAAGA+GGG	0.570209	8:-49291307	None:intergenic
AAATGCAAGCTACACAACTC+GGG	0.520511	8:+49290580	MsG0880044793.01.T01:CDS
AACTATTGCATCCAAATTCA+AGG	0.494397	8:+49287802	MsG0880044793.01.T01:CDS
AACTTGTAATGGTGATAATT+TGG	0.189699	8:+49290527	MsG0880044793.01.T01:CDS
AAGTTACCACCCATGCACTT+GGG	0.622055	8:+49291379	MsG0880044793.01.T01:CDS
AATAATTGATTCAGAGAACA+TGG	0.563794	8:+49289633	MsG0880044793.01.T01:CDS
AATAGGAGTCACTTGAAGAG+GGG	0.664295	8:-49291306	None:intergenic
AATCGTCTACGAAGAAGGAG+CGG	0.700661	8:+49286814	MsG0880044793.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTATTGCATCCAAATTCA+AGG	+	Chr8:49287802-49287821	MsG0880044793.01.T01:CDS	30.0%
AATAATACTTAAGCTTCACG+GGG	-	Chr8:49287349-49287368	None:intergenic	30.0%
AATGATTTCAGAAAGTAAGC+GGG	-	Chr8:49290735-49290754	None:intergenic	30.0%
AATGCTAATTTCAAGAAAGG+TGG	+	Chr8:49287974-49287993	MsG0880044793.01.T01:CDS	30.0%
AGAAAACAGAGAAAGTAAGT+GGG	+	Chr8:49291015-49291034	MsG0880044793.01.T01:intron	30.0%
AGAGCAAAAAAACAACAGTA+TGG	-	Chr8:49287542-49287561	None:intergenic	30.0%
ATCAAGAAATTAGGTCACAA+AGG	-	Chr8:49288871-49288890	None:intergenic	30.0%
ATTATCTGTTCATCACAGTT+TGG	-	Chr8:49291263-49291282	None:intergenic	30.0%
ATTGGATTGTTGAGCTAATT+CGG	-	Chr8:49289382-49289401	None:intergenic	30.0%
CAACATAAGTCAACTTGTAA+TGG	+	Chr8:49290516-49290535	MsG0880044793.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	49286777	49291406	49286777	ID=MsG0880044793.01;Name=MsG0880044793.01
Chr8	mRNA	49286777	49291406	49286777	ID=MsG0880044793.01.T01;Parent=MsG0880044793.01;Name=MsG0880044793.01.T01;_AED=0.44;_eAED=0.45;_QI=0\|0.7\|0.54\|1\|1\|1\|11\|0\|409
Chr8	exon	49286777	49286860	49286777	ID=MsG0880044793.01.T01:exon:20491;Parent=MsG0880044793.01.T01
Chr8	exon	49287460	49287506	49287460	ID=MsG0880044793.01.T01:exon:20492;Parent=MsG0880044793.01.T01
Chr8	exon	49287796	49287852	49287796	ID=MsG0880044793.01.T01:exon:20493;Parent=MsG0880044793.01.T01
Chr8	exon	49287958	49288030	49287958	ID=MsG0880044793.01.T01:exon:20494;Parent=MsG0880044793.01.T01
Chr8	exon	49288904	49288959	49288904	ID=MsG0880044793.01.T01:exon:20495;Parent=MsG0880044793.01.T01
Chr8	exon	49289049	49289198	49289049	ID=MsG0880044793.01.T01:exon:20496;Parent=MsG0880044793.01.T01
Chr8	exon	49289278	49289459	49289278	ID=MsG0880044793.01.T01:exon:20497;Parent=MsG0880044793.01.T01
Chr8	exon	49289575	49289669	49289575	ID=MsG0880044793.01.T01:exon:20498;Parent=MsG0880044793.01.T01
Chr8	exon	49290258	49290379	49290258	ID=MsG0880044793.01.T01:exon:20499;Parent=MsG0880044793.01.T01
Chr8	exon	49290455	49290663	49290455	ID=MsG0880044793.01.T01:exon:20500;Parent=MsG0880044793.01.T01
Chr8	exon	49291252	49291406	49291252	ID=MsG0880044793.01.T01:exon:20501;Parent=MsG0880044793.01.T01
Chr8	CDS	49286777	49286860	49286777	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49287460	49287506	49287460	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49287796	49287852	49287796	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49287958	49288030	49287958	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49288904	49288959	49288904	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49289049	49289198	49289049	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49289278	49289459	49289278	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49289575	49289669	49289575	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49290258	49290379	49290258	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49290455	49290663	49290455	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01
Chr8	CDS	49291252	49291406	49291252	ID=MsG0880044793.01.T01:cds;Parent=MsG0880044793.01.T01

Gene Sequence

>MsG0880044793.01.T01

ATGGATACGAAGAAGGAGAAGCAACAAGGGAATGAAGAATCGTCTACGAAGAAGGAGCGGCATATTGTGACTTGGACTCAAGAGGAGGATGATATACTAAGAGAACAGATCGGAAATCATGGAACTGAAAATTGGGCAACTATTGCATCCAAATTCAAGGACAAAACTACAAGACAATGCAGAAGAAGATGGTACACTTATTTGAATGCTAATTTCAAGAAAGGTGGATGGACACCAGACGAAGATATCCTCTTATGTGAGGCTCAAAAAATATTTGGAAACAGATGGACTGAAATAGCAAAGGTGGTTTCGGGAAGAACGGATAATGCTGTAAAAAATCGATTCTCCACACTCCGCAAGAAGAGGGGAAAAAATGACATTGATTCAAATAGCAAGAGGAATATTTCCTATCAAGATTATAATACAGATGCAATGTCAGAATCTGCCATGCCAGTTAAAAAAACGAGGACGGCCCATATCCCCGATGATGCAAAAAAGATCAACTTTGGAGACAGATCACATTTGCGAAATGCAATTTCATTAAATCAGCAGCCAAGAGCACCACTTGCCGAATTAGCTCAACAATCCAATAATGTGAACAATTTTCCAGACAGGCATCATGTTTCCAATGACAAGATCAGCAGTTCAGGCCAAAATAACAAATATGAACTCAAAAAGGATGATCCGAAGATAAGTGCATTGATTCAAATAATTGATTCAGAGAACATGGAAAGAGTATGGAAGCCACTTCTAGAGTTTCTGAACCAAACAGATATCATTGGAGAAAAAATTGCAGTTTTACAGCTTGTAAGTCATACAGTCAAGGAGTTTAAGAGTAGTAATGAGAGAGGCAATTCCCGCTTGAGGCAAATAAAACCAGACAAGGACTCCCCAGGAAGTTCTGAATACAGTACAGGATTAACTCTGCAACATAAGTCAACTTGTAATGGTGATAATTTGGAAAGCTCATTGAATCAGGATACTGGAACTGAAATGCAAGCTACACAACTCGGGGTTAAAAAAGAAGTATGTGAAGGTGTGAAAGAGGTTCTTTCTACTGGAAAAGTGGAACAAGATATGATACCAAACTGTGATGAACAGATAATTGCCTTGCCAAGAATGAACTGTTCCCCTCTTCAAGTGACTCCTATTTTCAGATCCTTGGCTGAAGGAATTCCAACCCCAGAATTTACAGACAGTGTAAGTTACCACCCATGCACTTGGGATTAA

Protein sequence

>MsG0880044793.01.T01

MDTKKEKQQGNEESSTKKERHIVTWTQEEDDILREQIGNHGTENWATIASKFKDKTTRQCRRRWYTYLNANFKKGGWTPDEDILLCEAQKIFGNRWTEIAKVVSGRTDNAVKNRFSTLRKKRGKNDIDSNSKRNISYQDYNTDAMSESAMPVKKTRTAHIPDDAKKINFGDRSHLRNAISLNQQPRAPLAELAQQSNNVNNFPDRHHVSNDKISSSGQNNKYELKKDDPKISALIQIIDSENMERVWKPLLEFLNQTDIIGEKIAVLQLVSHTVKEFKSSNERGNSRLRQIKPDKDSPGSSEYSTGLTLQHKSTCNGDNLESSLNQDTGTEMQATQLGVKKEVCEGVKEVLSTGKVEQDMIPNCDEQIIALPRMNCSPLQVTPIFRSLAEGIPTPEFTDSVSYHPCTWD*