AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044838.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044838.01.T01 MTR_8g469150 99.275 276 2 0 1 276 1 276 0.0 559
MsG0880044838.01.T01 MTR_8g469150 99.251 267 2 0 1 267 1 267 0.0 539
MsG0880044838.01.T01 MTR_6g011390 62.230 278 103 1 1 276 1 278 1.14e-127 364
MsG0880044838.01.T01 MTR_7g009330 58.394 274 114 0 3 276 4 277 4.94e-117 337
MsG0880044838.01.T01 MTR_5g015580 41.304 276 162 0 1 276 1 276 2.32e-76 234
MsG0880044838.01.T01 MTR_4g128300 41.219 279 161 2 1 276 1 279 2.61e-72 223
MsG0880044838.01.T01 MTR_1g018205 39.051 274 167 0 3 276 4 277 3.06e-68 213
MsG0880044838.01.T01 MTR_4g107840 34.629 283 139 5 1 276 1 244 5.01e-48 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044838.01.T01 AT3G01280 62.681 276 103 0 1 276 1 276 6.73e-127 362
MsG0880044838.01.T01 AT5G15090 59.058 276 111 1 1 276 1 274 4.47e-119 342
MsG0880044838.01.T01 AT5G15090 59.058 276 111 1 1 276 1 274 4.47e-119 342
MsG0880044838.01.T01 AT5G67500 42.754 276 158 0 1 276 1 276 5.27e-80 243
MsG0880044838.01.T01 AT5G57490 40.217 276 163 1 1 276 1 274 8.70e-76 232
MsG0880044838.01.T01 AT5G67500 42.424 264 152 0 1 264 1 264 1.32e-74 230
MsG0880044838.01.T01 AT5G67500 38.944 303 158 1 1 276 1 303 1.21e-73 228
MsG0880044838.01.T01 AT3G49920 32.609 276 136 2 1 276 1 226 4.99e-45 152
MsG0880044838.01.T01 AT3G49920 50.000 82 41 0 1 82 1 82 1.93e-21 87.8
MsG0880044838.01.T01 AT3G49920 50.000 82 41 0 1 82 1 82 1.93e-21 87.8
MsG0880044838.01.T01 AT3G49920 45.679 81 44 0 1 81 1 81 4.55e-20 83.2
MsG0880044838.01.T01 AT5G37610 29.730 148 100 2 111 256 2 147 5.50e-16 73.9

Find 90 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGAACAAGTGCAATTT+TGG 0.126845 8:+49963624 MsG0880044838.01.T01:CDS
ACCTTTGGTGTCCTTCATTT+TGG 0.168290 8:+49964210 MsG0880044838.01.T01:three_prime_UTR
CTTCATATCTTGGTTGGTTC+AGG 0.196641 8:+49964246 MsG0880044838.01.T01:three_prime_UTR
TGCTGTGCTTGGAAATAATT+TGG 0.214953 8:+49963560 MsG0880044838.01.T01:CDS
ATCACAACTCAAACTTTATC+TGG 0.280321 8:+49961587 MsG0880044838.01.T01:CDS
TTAGCTTTCAACAAGGTTAC+AGG 0.284651 8:-49963962 None:intergenic
GTTTCCTTTGAGACTTCTTC+TGG 0.285886 8:+49963600 MsG0880044838.01.T01:CDS
TTACAAAATCACCAATTCTA+AGG 0.292201 8:-49964121 None:intergenic
CAAGCACGTTTCTCTCTTGT+TGG 0.302532 8:+49964037 MsG0880044838.01.T01:CDS
GGTTAGCACGTTCTCATTGC+TGG 0.309092 8:-49963915 None:intergenic
TATTAAATTTCTTCATATCT+TGG 0.309983 8:+49964236 MsG0880044838.01.T01:three_prime_UTR
AATTCCACCTGAACCTTCAT+TGG 0.317322 8:+49964319 MsG0880044838.01.T01:three_prime_UTR
AAAGCTAAGGTGAACAATTA+TGG 0.329757 8:+49963977 MsG0880044838.01.T01:CDS
TTCACTTTCCCATGAACCTT+TGG 0.330184 8:+49964195 MsG0880044838.01.T01:three_prime_UTR
CCCAAAATTGCACTTGTTCA+AGG 0.352183 8:-49963625 None:intergenic
CACTGACAAACACTGCTGTT+GGG 0.370441 8:+49963870 MsG0880044838.01.T01:CDS
TTCATATCTTGGTTGGTTCA+GGG 0.372386 8:+49964247 MsG0880044838.01.T01:three_prime_UTR
AATTGATAGGTTGCTTTGTC+TGG 0.373816 8:+49964142 MsG0880044838.01.T01:three_prime_UTR
TGCGAGAAAATGTGAGAAAA+TGG 0.384707 8:-49961346 None:intergenic
AAGCACGTTTCTCTCTTGTT+GGG 0.384862 8:+49964038 MsG0880044838.01.T01:CDS
GGAAATAATTTGGTTTCTGT+TGG 0.384877 8:+49963570 MsG0880044838.01.T01:CDS
TCGAGCTCTCTTGCCGATAT+CGG 0.395739 8:-49961426 None:intergenic
GTGCTCTGATCCAGCATGAC+TGG 0.400701 8:+49964008 MsG0880044838.01.T01:CDS
CACGTTCTCATTGCTGGAAA+AGG 0.403866 8:-49963909 None:intergenic
ATTCAGGAAATTACTTCAGC+TGG 0.407698 8:+49962211 MsG0880044838.01.T01:intron
CCTGGTCTTTACTCCGATAT+CGG 0.414464 8:+49961413 MsG0880044838.01.T01:CDS
GTGCCTTGAAGCCTTAGAAT+TGG 0.415257 8:+49964110 MsG0880044838.01.T01:exon
CTGGGATCAGTACAAGTATT+GGG 0.431393 8:+49963508 MsG0880044838.01.T01:CDS
TCACCAATTCTAAGGCTTCA+AGG 0.444450 8:-49964113 None:intergenic
TGAAGGACACCAAAGGTTCA+TGG 0.449050 8:-49964204 None:intergenic
TGGTGAACTTGTGATCATTC+TGG 0.451800 8:-49961565 None:intergenic
CACTGTTTCATCCACAGTAA+TGG 0.455738 8:-49963254 None:intergenic
AAATTACTTCAGCTGGAGTC+AGG 0.460813 8:+49962218 MsG0880044838.01.T01:CDS
CTTGTCGGCTCCAGTCATGC+TGG 0.463299 8:-49964018 None:intergenic
GAGACAATAAGTTTAGTGGT+AGG 0.463718 8:+49964170 MsG0880044838.01.T01:three_prime_UTR
CAAGAGAGAAACGTGCTTGT+CGG 0.468813 8:-49964033 None:intergenic
AAATTTCTTCATATCTTGGT+TGG 0.470349 8:+49964240 MsG0880044838.01.T01:three_prime_UTR
AAAATCATGGTGAAGGGTCC+TGG 0.470528 8:+49961395 MsG0880044838.01.T01:exon
TCGATTGTATATCGAAGCTC+TGG 0.472594 8:+49964407 MsG0880044838.01.T01:three_prime_UTR
ACACAGTCAGAGAAGCAATA+AGG 0.479979 8:-49963668 None:intergenic
CAGTACCTTCATGATTATGC+TGG 0.480670 8:+49963489 MsG0880044838.01.T01:CDS
CCACTGACAAACACTGCTGT+TGG 0.481681 8:+49963869 MsG0880044838.01.T01:CDS
GGTGGAGACAATAAGTTTAG+TGG 0.481798 8:+49964166 MsG0880044838.01.T01:three_prime_UTR
ATGGTAATACGAGGCATTGA+GGG 0.487946 8:-49963837 None:intergenic
AACCTTTGCAGTCTTCTGAA+TGG 0.490461 8:-49964077 None:intergenic
TAGGTTGCTTTGTCTGGTGG+TGG 0.495597 8:+49964148 MsG0880044838.01.T01:three_prime_UTR
GTTAACATCTCTGCTGTGCT+TGG 0.505642 8:+49963549 MsG0880044838.01.T01:CDS
GCTGGGATCAGTACAAGTAT+TGG 0.506695 8:+49963507 MsG0880044838.01.T01:CDS
CAACAGCAGTGTTTGTCAGT+GGG 0.513206 8:-49963868 None:intergenic
ACTGACAAACACTGCTGTTG+GGG 0.515446 8:+49963871 MsG0880044838.01.T01:CDS
ATCTAAACCAATGAAGGTTC+AGG 0.515984 8:-49964326 None:intergenic
AGACAATAAGTTTAGTGGTA+GGG 0.519800 8:+49964171 MsG0880044838.01.T01:three_prime_UTR
AGGTGCATCTAAACCAATGA+AGG 0.523225 8:-49964332 None:intergenic
ATTTAATAGCTCCAAAATGA+AGG 0.523736 8:-49964221 None:intergenic
TAAGGTGAACAATTATGGTA+AGG 0.534887 8:+49963982 MsG0880044838.01.T01:CDS
GAAGGACACCAAAGGTTCAT+GGG 0.535930 8:-49964203 None:intergenic
ACTGTGGATGAAACAGTGCC+TGG 0.542506 8:+49963259 MsG0880044838.01.T01:CDS
CATGGTAATACGAGGCATTG+AGG 0.543948 8:-49963838 None:intergenic
CCGATATCGGAGTAAAGACC+AGG 0.547394 8:-49961413 None:intergenic
GACGTTCTTGTTCTTCACCT+TGG 0.552259 8:-49962272 None:intergenic
TGATAGGTTGCTTTGTCTGG+TGG 0.554785 8:+49964145 MsG0880044838.01.T01:three_prime_UTR
AGCACGTTTCTCTCTTGTTG+GGG 0.556784 8:+49964039 MsG0880044838.01.T01:CDS
GGAGCCAGAAGAAGTCTCAA+AGG 0.559723 8:-49963604 None:intergenic
AATGAGAACGTGCTAACCAT+TGG 0.560059 8:+49963920 MsG0880044838.01.T01:CDS
AACTCAAACTTTATCTGGAG+TGG 0.567321 8:+49961592 MsG0880044838.01.T01:CDS
CCAACAGCAGTGTTTGTCAG+TGG 0.567351 8:-49963869 None:intergenic
GATGAATGTAGCAATGATAG+AGG 0.573898 8:+49963809 MsG0880044838.01.T01:intron
GACAAACACTGCTGTTGGGG+CGG 0.573950 8:+49963874 MsG0880044838.01.T01:CDS
AGTACCTTCATGATTATGCT+GGG 0.579555 8:+49963490 MsG0880044838.01.T01:CDS
TGTAACCTTGTTGAAAGCTA+AGG 0.579891 8:+49963964 MsG0880044838.01.T01:CDS
ATTCAGAAGACTGCAAAGGT+TGG 0.586239 8:+49964079 MsG0880044838.01.T01:CDS
ACTTCAGCTGGAGTCAGGAA+AGG 0.601532 8:+49962223 MsG0880044838.01.T01:CDS
AAGAGCATGTTGTGTGCCAA+TGG 0.601630 8:-49963936 None:intergenic
TTGTCAGTGGGCTCACAACA+TGG 0.603253 8:-49963856 None:intergenic
TGATCCCAGCATAATCATGA+AGG 0.608651 8:-49963494 None:intergenic
TAAACCAATGAAGGTTCAGG+TGG 0.609371 8:-49964323 None:intergenic
CACAGCAGAGATGTTAACAG+TGG 0.610351 8:-49963544 None:intergenic
TAGCTTTCAACAAGGTTACA+GGG 0.614278 8:-49963961 None:intergenic
AGATAAAGTTTGAGTTGTGA+TGG 0.614597 8:-49961585 None:intergenic
GGTGCAAAATCAATAAGCAC+AGG 0.617626 8:+49964268 MsG0880044838.01.T01:three_prime_UTR
TGCCATTCAGAAGACTGCAA+AGG 0.631852 8:+49964075 MsG0880044838.01.T01:CDS
ACAGCAGAGATGTTAACAGT+GGG 0.643117 8:-49963543 None:intergenic
TCCAAAATGAAGGACACCAA+AGG 0.648587 8:-49964211 None:intergenic
AGCTGGAGTCAGGAAAGGTG+AGG 0.649229 8:+49962228 MsG0880044838.01.T01:CDS
TTTCTCGAGAAAGTGAAGAG+CGG 0.655417 8:-49961373 None:intergenic
GATATCGGCAAGAGAGCTCG+AGG 0.667893 8:+49961428 MsG0880044838.01.T01:CDS
GTTCACCTTAGCTTTCAACA+AGG 0.681029 8:-49963969 None:intergenic
GCTGCGCACAACCATTACTG+TGG 0.700222 8:+49963243 MsG0880044838.01.T01:intron
TTTGGCTGATGTGAGTACCA+AGG 0.717476 8:+49962255 MsG0880044838.01.T01:CDS
GCTCACAACATGGTAATACG+AGG 0.740689 8:-49963846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGTTTTTAGTTTTAAAT+CGG + Chr8:49961831-49961850 MsG0880044838.01.T01:intron 10.0%
!!! CAAATTTTTTCATTTTTTTT+TGG + Chr8:49962530-49962549 MsG0880044838.01.T01:intron 10.0%
!! AATTCTTAATAACAATATCA+TGG - Chr8:49962883-49962902 None:intergenic 15.0%
!! AGATTATACAAAAATTATTC+GGG - Chr8:49963438-49963457 None:intergenic 15.0%
!! GAGATTATACAAAAATTATT+CGG - Chr8:49963439-49963458 None:intergenic 15.0%
!! TAGTTTCTTAAAATTTAAGA+TGG + Chr8:49962437-49962456 MsG0880044838.01.T01:intron 15.0%
!! TATTAAATTTCTTCATATCT+TGG + Chr8:49964236-49964255 MsG0880044838.01.T01:three_prime_UTR 15.0%
!!! ACAATTTAGCTTTATTTTAT+CGG - Chr8:49962657-49962676 None:intergenic 15.0%
!!! ATAGTTTTTAGTTTTAAATC+GGG + Chr8:49961832-49961851 MsG0880044838.01.T01:intron 15.0%
!!! ATCTTTTATTTTTTGGTTTT+TGG + Chr8:49962497-49962516 MsG0880044838.01.T01:intron 15.0%
!!! ATGAAACTTTTTTGAAATAA+TGG + Chr8:49961752-49961771 MsG0880044838.01.T01:intron 15.0%
!!! CAACTTAATTTGAATTTTAT+AGG + Chr8:49962580-49962599 MsG0880044838.01.T01:intron 15.0%
!!! CTACTATGTTAAAATTTTAA+AGG - Chr8:49964486-49964505 None:intergenic 15.0%
!!! GATTTTTTTTTTAGTTAAAG+TGG + Chr8:49961861-49961880 MsG0880044838.01.T01:intron 15.0%
!! AAAAAACAGAACAAATAATC+AGG - Chr8:49963786-49963805 None:intergenic 20.0%
!! AGTATCAACAAATTCAATAT+TGG - Chr8:49963402-49963421 None:intergenic 20.0%
!! TAAGTTGTTTATCTAAACAA+AGG - Chr8:49962567-49962586 None:intergenic 20.0%
!!! AAGCTAAATTGTTTTCTTAT+TGG + Chr8:49962665-49962684 MsG0880044838.01.T01:intron 20.0%
!!! AGCTTGAATTTTTTATTCTT+TGG + Chr8:49962140-49962159 MsG0880044838.01.T01:intron 20.0%
!!! CTAATTTTACAATTGCAAAA+TGG - Chr8:49962385-49962404 None:intergenic 20.0%
!!! TAGTTTTTAGTTTTAAATCG+GGG + Chr8:49961833-49961852 MsG0880044838.01.T01:intron 20.0%
!!! TTTTTACTTGAAATAACACT+TGG + Chr8:49962355-49962374 MsG0880044838.01.T01:intron 20.0%
! AAATTAGTAGTTGCATTGTT+TGG + Chr8:49962398-49962417 MsG0880044838.01.T01:intron 25.0%
! AAATTATTCGGGATGAAAAT+TGG - Chr8:49963427-49963446 None:intergenic 25.0%
! AAATTTCTTCATATCTTGGT+TGG + Chr8:49964240-49964259 MsG0880044838.01.T01:three_prime_UTR 25.0%
! AATTATTCGGGATGAAAATT+GGG - Chr8:49963426-49963445 None:intergenic 25.0%
! ACAACTAATTGTATAAGACT+TGG - Chr8:49963029-49963048 None:intergenic 25.0%
! ACAACTTATTGTATAAGACT+TGG - Chr8:49963161-49963180 None:intergenic 25.0%
! ACTGAAATTGTAAAGAATGA+AGG - Chr8:49961463-49961482 None:intergenic 25.0%
! ATTGTAAAGAATGAAGGAAT+CGG - Chr8:49961457-49961476 None:intergenic 25.0%
! ATTTAATAGCTCCAAAATGA+AGG - Chr8:49964224-49964243 None:intergenic 25.0%
! TTACAAAATCACCAATTCTA+AGG - Chr8:49964124-49964143 None:intergenic 25.0%
!!! CATTCTGAAATATAAACTTG+AGG - Chr8:49962062-49962081 None:intergenic 25.0%
!!! TCTTTGGTTTGTTATAAAAG+CGG + Chr8:49962156-49962175 MsG0880044838.01.T01:intron 25.0%
!!! TGGTTTTAATTGGTTTATTC+AGG + Chr8:49962195-49962214 MsG0880044838.01.T01:intron 25.0%
!!! TTGGTGATTTTGTAATTGAT+AGG + Chr8:49964129-49964148 MsG0880044838.01.T01:three_prime_UTR 25.0%
AAAGCTAAGGTGAACAATTA+TGG + Chr8:49963977-49963996 MsG0880044838.01.T01:CDS 30.0%
ACTTTCTCGAGAAAAAATCA+TGG + Chr8:49961382-49961401 MsG0880044838.01.T01:exon 30.0%
AGACAATAAGTTTAGTGGTA+GGG + Chr8:49964171-49964190 MsG0880044838.01.T01:three_prime_UTR 30.0%
AGTCTCATTAGTGTCAATTT+CGG + Chr8:49961982-49962001 MsG0880044838.01.T01:intron 30.0%
ATCACAACTCAAACTTTATC+TGG + Chr8:49961587-49961606 MsG0880044838.01.T01:CDS 30.0%
ATGCTCAAAAACTTACTCAA+AGG - Chr8:49964355-49964374 None:intergenic 30.0%
ATTTATCCAGAGCAAAAATC+TGG + Chr8:49963304-49963323 MsG0880044838.01.T01:CDS 30.0%
CAGATGATTATGTTGTAGAA+TGG - Chr8:49962860-49962879 None:intergenic 30.0%
CCATGATTTCAAGAAAAATG+TGG - Chr8:49962972-49962991 None:intergenic 30.0%
GTCATGTACAATGAAACAAA+GGG - Chr8:49962838-49962857 None:intergenic 30.0%
GTCTTATACAATTAGTTGTC+TGG + Chr8:49963030-49963049 MsG0880044838.01.T01:intron 30.0%
TAAGGTGAACAATTATGGTA+AGG + Chr8:49963982-49964001 MsG0880044838.01.T01:CDS 30.0%
TAGAAAACAACAACAAACCA+GGG - Chr8:49961683-49961702 None:intergenic 30.0%
TGATTACACAACTCATTTCA+TGG + Chr8:49963724-49963743 MsG0880044838.01.T01:intron 30.0%
TTGTATAATCTCTCCTTCTT+TGG + Chr8:49963447-49963466 MsG0880044838.01.T01:intron 30.0%
! CCACATTTTTCTTGAAATCA+TGG + Chr8:49962969-49962988 MsG0880044838.01.T01:intron 30.0%
! GGAAATAATTTGGTTTCTGT+TGG + Chr8:49963570-49963589 MsG0880044838.01.T01:CDS 30.0%
! TGTTGCATGTTTTACATTTC+AGG + Chr8:49961708-49961727 MsG0880044838.01.T01:intron 30.0%
!! AGATAAAGTTTGAGTTGTGA+TGG - Chr8:49961588-49961607 None:intergenic 30.0%
!! TTTGAGTAAGTTTTTGAGCA+TGG + Chr8:49964354-49964373 MsG0880044838.01.T01:three_prime_UTR 30.0%
!!! TGGTTTGTTGTTGTTTTCTA+GGG + Chr8:49961683-49961702 MsG0880044838.01.T01:intron 30.0%
AACTCAAACTTTATCTGGAG+TGG + Chr8:49961592-49961611 MsG0880044838.01.T01:CDS 35.0%
AAGCAATTGTGAGACTGTTT+GGG - Chr8:49962773-49962792 None:intergenic 35.0%
ACTGTAGTTGTGAGAATTGT+TGG + Chr8:49962115-49962134 MsG0880044838.01.T01:intron 35.0%
AGTACCTTCATGATTATGCT+GGG + Chr8:49963490-49963509 MsG0880044838.01.T01:CDS 35.0%
ATATCATAAACAAGCAACCC+AGG - Chr8:49962474-49962493 None:intergenic 35.0%
ATCTAAACCAATGAAGGTTC+AGG - Chr8:49964329-49964348 None:intergenic 35.0%
ATTCAGGAAATTACTTCAGC+TGG + Chr8:49962211-49962230 MsG0880044838.01.T01:intron 35.0%
CACCTATAAAACTCCAAAGA+AGG - Chr8:49963463-49963482 None:intergenic 35.0%
CGAGAAAAAATCATGGTGAA+GGG + Chr8:49961389-49961408 MsG0880044838.01.T01:exon 35.0%
CTAAAAATTGTCTTGAGTCC+AGG - Chr8:49963280-49963299 None:intergenic 35.0%
CTAGAAAACAACAACAAACC+AGG - Chr8:49961684-49961703 None:intergenic 35.0%
CTGTGTAAGTAGTTGTCTAA+AGG + Chr8:49963685-49963704 MsG0880044838.01.T01:intron 35.0%
GAGACAATAAGTTTAGTGGT+AGG + Chr8:49964170-49964189 MsG0880044838.01.T01:three_prime_UTR 35.0%
GATGAATGTAGCAATGATAG+AGG + Chr8:49963809-49963828 MsG0880044838.01.T01:intron 35.0%
GGTCATGTACAATGAAACAA+AGG - Chr8:49962839-49962858 None:intergenic 35.0%
TAAGCAATTGTGAGACTGTT+TGG - Chr8:49962774-49962793 None:intergenic 35.0%
TAGCTTTCAACAAGGTTACA+GGG - Chr8:49963964-49963983 None:intergenic 35.0%
TATCATAAACAAGCAACCCA+GGG - Chr8:49962473-49962492 None:intergenic 35.0%
TCGAGAAAAAATCATGGTGA+AGG + Chr8:49961388-49961407 MsG0880044838.01.T01:exon 35.0%
TGCGAGAAAATGTGAGAAAA+TGG - Chr8:49961349-49961368 None:intergenic 35.0%
TGCTGTGCTTGGAAATAATT+TGG + Chr8:49963560-49963579 MsG0880044838.01.T01:CDS 35.0%
TGTAACCTTGTTGAAAGCTA+AGG + Chr8:49963964-49963983 MsG0880044838.01.T01:CDS 35.0%
TGTATTCGTAAGCATCTGTT+TGG + Chr8:49962930-49962949 MsG0880044838.01.T01:intron 35.0%
TGTTATAAAAGCGGAGGTTT+CGG + Chr8:49962165-49962184 MsG0880044838.01.T01:intron 35.0%
! AATTGATAGGTTGCTTTGTC+TGG + Chr8:49964142-49964161 MsG0880044838.01.T01:three_prime_UTR 35.0%
! CCTTGAACAAGTGCAATTTT+GGG + Chr8:49963625-49963644 MsG0880044838.01.T01:CDS 35.0%
! TCCTTGAACAAGTGCAATTT+TGG + Chr8:49963624-49963643 MsG0880044838.01.T01:CDS 35.0%
! TTAGCTTTCAACAAGGTTAC+AGG - Chr8:49963965-49963984 None:intergenic 35.0%
! TTCATATCTTGGTTGGTTCA+GGG + Chr8:49964247-49964266 MsG0880044838.01.T01:three_prime_UTR 35.0%
! TTGGTCATGTAATTTTTCCC+TGG + Chr8:49961663-49961682 MsG0880044838.01.T01:intron 35.0%
!! GTAAGTTTTTGAGCATGGAA+AGG + Chr8:49964359-49964378 MsG0880044838.01.T01:three_prime_UTR 35.0%
!! TTGGTTTGTTATAAAAGCGG+AGG + Chr8:49962159-49962178 MsG0880044838.01.T01:intron 35.0%
!!! CGGTGTTTGATGGTTTTAAT+TGG + Chr8:49962185-49962204 MsG0880044838.01.T01:intron 35.0%
!!! CTCCTTCTTTGGAGTTTTAT+AGG + Chr8:49963458-49963477 MsG0880044838.01.T01:intron 35.0%
!!! CTGGTTTGTTGTTGTTTTCT+AGG + Chr8:49961682-49961701 MsG0880044838.01.T01:intron 35.0%
!!! CTTCTTTGGAGTTTTATAGG+TGG + Chr8:49963461-49963480 MsG0880044838.01.T01:intron 35.0%
AAAGGGCTTGACTCTTAGAT+TGG - Chr8:49962821-49962840 None:intergenic 40.0%
AAATTACTTCAGCTGGAGTC+AGG + Chr8:49962218-49962237 MsG0880044838.01.T01:CDS 40.0%
AACCTTTGCAGTCTTCTGAA+TGG - Chr8:49964080-49964099 None:intergenic 40.0%
AAGATGGAATTCCATTTCCC+TGG + Chr8:49962453-49962472 MsG0880044838.01.T01:intron 40.0%
AAGCACGTTTCTCTCTTGTT+GGG + Chr8:49964038-49964057 MsG0880044838.01.T01:CDS 40.0%
AATGAGAACGTGCTAACCAT+TGG + Chr8:49963920-49963939 MsG0880044838.01.T01:CDS 40.0%
AATTCCACCTGAACCTTCAT+TGG + Chr8:49964319-49964338 MsG0880044838.01.T01:three_prime_UTR 40.0%
ACACAGTCAGAGAAGCAATA+AGG - Chr8:49963671-49963690 None:intergenic 40.0%
ACAGCAGAGATGTTAACAGT+GGG - Chr8:49963546-49963565 None:intergenic 40.0%
AGATGGAATTCCATTTCCCT+GGG + Chr8:49962454-49962473 MsG0880044838.01.T01:intron 40.0%
AGGTGCATCTAAACCAATGA+AGG - Chr8:49964335-49964354 None:intergenic 40.0%
ATGTTAACGCAGATCCAAAC+AGG + Chr8:49963001-49963020 MsG0880044838.01.T01:intron 40.0%
ATTCAGAAGACTGCAAAGGT+TGG + Chr8:49964079-49964098 MsG0880044838.01.T01:CDS 40.0%
ATTTGAACTCGAAGCTTACG+AGG + Chr8:49961918-49961937 MsG0880044838.01.T01:intron 40.0%
CACTGTTTCATCCACAGTAA+TGG - Chr8:49963257-49963276 None:intergenic 40.0%
CAGTACCTTCATGATTATGC+TGG + Chr8:49963489-49963508 MsG0880044838.01.T01:CDS 40.0%
CCCAAAATTGCACTTGTTCA+AGG - Chr8:49963628-49963647 None:intergenic 40.0%
CTGCATTTCATCCACAGTAA+TGG - Chr8:49963125-49963144 None:intergenic 40.0%
CTGGGATCAGTACAAGTATT+GGG + Chr8:49963508-49963527 MsG0880044838.01.T01:CDS 40.0%
GATGAAATGCAGATCCAAAC+AGG + Chr8:49963133-49963152 MsG0880044838.01.T01:intron 40.0%
GGTGCAAAATCAATAAGCAC+AGG + Chr8:49964268-49964287 MsG0880044838.01.T01:three_prime_UTR 40.0%
GGTGGAGACAATAAGTTTAG+TGG + Chr8:49964166-49964185 MsG0880044838.01.T01:three_prime_UTR 40.0%
TAAACCAATGAAGGTTCAGG+TGG - Chr8:49964326-49964345 None:intergenic 40.0%
TCAATTTCGGCTGTCTAGTT+TGG + Chr8:49961995-49962014 MsG0880044838.01.T01:intron 40.0%
TCCAAAATGAAGGACACCAA+AGG - Chr8:49964214-49964233 None:intergenic 40.0%
TCGATTGTATATCGAAGCTC+TGG + Chr8:49964407-49964426 MsG0880044838.01.T01:three_prime_UTR 40.0%
TGATCCCAGCATAATCATGA+AGG - Chr8:49963497-49963516 None:intergenic 40.0%
TGGTGAACTTGTGATCATTC+TGG - Chr8:49961568-49961587 None:intergenic 40.0%
TGTTTGGATCTGCGTTAACA+TGG - Chr8:49963002-49963021 None:intergenic 40.0%
TTCACTTTCCCATGAACCTT+TGG + Chr8:49964195-49964214 MsG0880044838.01.T01:three_prime_UTR 40.0%
TTTCTCGAGAAAGTGAAGAG+CGG - Chr8:49961376-49961395 None:intergenic 40.0%
! AGTTTGGGTTCGTCAATAAG+AGG + Chr8:49962011-49962030 MsG0880044838.01.T01:intron 40.0%
! CAATTTCGGCTGTCTAGTTT+GGG + Chr8:49961996-49962015 MsG0880044838.01.T01:intron 40.0%
! CTTCATATCTTGGTTGGTTC+AGG + Chr8:49964246-49964265 MsG0880044838.01.T01:three_prime_UTR 40.0%
! GTTCACCTTAGCTTTCAACA+AGG - Chr8:49963972-49963991 None:intergenic 40.0%
!! AGTCATTTTACTACCGTCTC+CGG + Chr8:49961891-49961910 MsG0880044838.01.T01:intron 40.0%
!! ATGGTAATACGAGGCATTGA+GGG - Chr8:49963840-49963859 None:intergenic 40.0%
!! GCTGTTTTCATAAGCTATCC+TGG + Chr8:49962694-49962713 MsG0880044838.01.T01:intron 40.0%
!! GTTTCCTTTGAGACTTCTTC+TGG + Chr8:49963600-49963619 MsG0880044838.01.T01:CDS 40.0%
!! TATAAGACTTGGTGCCTGTT+TGG - Chr8:49963150-49963169 None:intergenic 40.0%
!! TCACCAATTCTAAGGCTTCA+AGG - Chr8:49964116-49964135 None:intergenic 40.0%
!!! ACCTTTGGTGTCCTTCATTT+TGG + Chr8:49964210-49964229 MsG0880044838.01.T01:three_prime_UTR 40.0%
AAAATCATGGTGAAGGGTCC+TGG + Chr8:49961395-49961414 MsG0880044838.01.T01:exon 45.0%
AAACAAGCAACCCAGGGAAA+TGG - Chr8:49962467-49962486 None:intergenic 45.0%
AATGGTTGTGTGCAGGTGAA+TGG - Chr8:49963107-49963126 None:intergenic 45.0%
ACTGACAAACACTGCTGTTG+GGG + Chr8:49963871-49963890 MsG0880044838.01.T01:CDS 45.0%
AGCACGTTTCTCTCTTGTTG+GGG + Chr8:49964039-49964058 MsG0880044838.01.T01:CDS 45.0%
ATGAGTATGACCTTCTCACG+AGG + Chr8:49961948-49961967 MsG0880044838.01.T01:intron 45.0%
CAACAGCAGTGTTTGTCAGT+GGG - Chr8:49963871-49963890 None:intergenic 45.0%
CAAGAGAGAAACGTGCTTGT+CGG - Chr8:49964036-49964055 None:intergenic 45.0%
CAAGCACGTTTCTCTCTTGT+TGG + Chr8:49964037-49964056 MsG0880044838.01.T01:CDS 45.0%
CACAGCAGAGATGTTAACAG+TGG - Chr8:49963547-49963566 None:intergenic 45.0%
CACGTTCTCATTGCTGGAAA+AGG - Chr8:49963912-49963931 None:intergenic 45.0%
CACTGACAAACACTGCTGTT+GGG + Chr8:49963870-49963889 MsG0880044838.01.T01:CDS 45.0%
GAAGGACACCAAAGGTTCAT+GGG - Chr8:49964206-49964225 None:intergenic 45.0%
GACGTTCTTGTTCTTCACCT+TGG - Chr8:49962275-49962294 None:intergenic 45.0%
GCTCACAACATGGTAATACG+AGG - Chr8:49963849-49963868 None:intergenic 45.0%
GCTGGGATCAGTACAAGTAT+TGG + Chr8:49963507-49963526 MsG0880044838.01.T01:CDS 45.0%
GTGCCTTGAAGCCTTAGAAT+TGG + Chr8:49964110-49964129 MsG0880044838.01.T01:exon 45.0%
GTTAACATCTCTGCTGTGCT+TGG + Chr8:49963549-49963568 MsG0880044838.01.T01:CDS 45.0%
TATAAGACTTGGCGCCTGTT+TGG - Chr8:49963018-49963037 None:intergenic 45.0%
TCCAGAGCAAAAATCTGGCA+AGG + Chr8:49963309-49963328 MsG0880044838.01.T01:CDS 45.0%
TGAAGGACACCAAAGGTTCA+TGG - Chr8:49964207-49964226 None:intergenic 45.0%
TGCCATTCAGAAGACTGCAA+AGG + Chr8:49964075-49964094 MsG0880044838.01.T01:CDS 45.0%
TTTGGCTGATGTGAGTACCA+AGG + Chr8:49962255-49962274 MsG0880044838.01.T01:CDS 45.0%
! AAGAGCATGTTGTGTGCCAA+TGG - Chr8:49963939-49963958 None:intergenic 45.0%
! ACCTTGCCAGATTTTTGCTC+TGG - Chr8:49963313-49963332 None:intergenic 45.0%
! ATTTTACTACCGTCTCCGGT+GGG + Chr8:49961895-49961914 MsG0880044838.01.T01:intron 45.0%
! CCTGGTCTTTACTCCGATAT+CGG + Chr8:49961413-49961432 MsG0880044838.01.T01:CDS 45.0%
! TAAACTTGAGGTGCTAGCTC+AGG - Chr8:49962050-49962069 None:intergenic 45.0%
! TGATAGGTTGCTTTGTCTGG+TGG + Chr8:49964145-49964164 MsG0880044838.01.T01:three_prime_UTR 45.0%
!! CAGGAAAGGTGAGGCATTTT+TGG + Chr8:49962237-49962256 MsG0880044838.01.T01:CDS 45.0%
!! CATGGTAATACGAGGCATTG+AGG - Chr8:49963841-49963860 None:intergenic 45.0%
!!! GCTTTTTTCAGAAGCTCTCC+AGG - Chr8:49962715-49962734 None:intergenic 45.0%
!!! ATATATTATCTTTTATTTTT+TGG + Chr8:49962490-49962509 MsG0880044838.01.T01:intron 5.0%
!!! TTTTATTTTTTTTAAGAATT+TGG + Chr8:49961644-49961663 MsG0880044838.01.T01:intron 5.0%
AATGGTTGTGCGCAGCTGAA+TGG - Chr8:49963239-49963258 None:intergenic 50.0%
ACTGTGGATGAAACAGTGCC+TGG + Chr8:49963259-49963278 MsG0880044838.01.T01:CDS 50.0%
ACTTCAGCTGGAGTCAGGAA+AGG + Chr8:49962223-49962242 MsG0880044838.01.T01:CDS 50.0%
AGTTCAAATCCCACCGGAGA+CGG - Chr8:49961907-49961926 None:intergenic 50.0%
CCAACAGCAGTGTTTGTCAG+TGG - Chr8:49963872-49963891 None:intergenic 50.0%
CCACAGTAATGGTTGTGTGC+AGG - Chr8:49963114-49963133 None:intergenic 50.0%
CCACTGACAAACACTGCTGT+TGG + Chr8:49963869-49963888 MsG0880044838.01.T01:CDS 50.0%
CCGATATCGGAGTAAAGACC+AGG - Chr8:49961416-49961435 None:intergenic 50.0%
CCTGCACACAACCATTACTG+TGG + Chr8:49963111-49963130 MsG0880044838.01.T01:intron 50.0%
GGAGCCAGAAGAAGTCTCAA+AGG - Chr8:49963607-49963626 None:intergenic 50.0%
GGTTAGCACGTTCTCATTGC+TGG - Chr8:49963918-49963937 None:intergenic 50.0%
TCGAGCTCTCTTGCCGATAT+CGG - Chr8:49961429-49961448 None:intergenic 50.0%
! CATTTTACTACCGTCTCCGG+TGG + Chr8:49961894-49961913 MsG0880044838.01.T01:intron 50.0%
! GCTTCGAGTTCAAATCCCAC+CGG - Chr8:49961913-49961932 None:intergenic 50.0%
! TAGGTTGCTTTGTCTGGTGG+TGG + Chr8:49964148-49964167 MsG0880044838.01.T01:three_prime_UTR 50.0%
! TTGTCAGTGGGCTCACAACA+TGG - Chr8:49963859-49963878 None:intergenic 50.0%
AGCTGGAGTCAGGAAAGGTG+AGG + Chr8:49962228-49962247 MsG0880044838.01.T01:CDS 55.0%
GAACCTGAGACCTCGTGAGA+AGG - Chr8:49961961-49961980 None:intergenic 55.0%
GACAAACACTGCTGTTGGGG+CGG + Chr8:49963874-49963893 MsG0880044838.01.T01:CDS 55.0%
GATATCGGCAAGAGAGCTCG+AGG + Chr8:49961428-49961447 MsG0880044838.01.T01:CDS 55.0%
GCTGCGCACAACCATTACTG+TGG + Chr8:49963243-49963262 MsG0880044838.01.T01:intron 55.0%
GTGCTCTGATCCAGCATGAC+TGG + Chr8:49964008-49964027 MsG0880044838.01.T01:CDS 55.0%
TGACCTTCTCACGAGGTCTC+AGG + Chr8:49961955-49961974 MsG0880044838.01.T01:intron 55.0%
!! GCGGAGGTTTCGGTGTTTGA+TGG + Chr8:49962175-49962194 MsG0880044838.01.T01:intron 55.0%
!! CTTGTCGGCTCCAGTCATGC+TGG - Chr8:49964021-49964040 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 49961343 49964517 49961343 ID=MsG0880044838.01;Name=MsG0880044838.01
Chr8 mRNA 49961343 49964517 49961343 ID=MsG0880044838.01.T01;Parent=MsG0880044838.01;Name=MsG0880044838.01.T01;_AED=0.49;_eAED=0.49;_QI=58|1|1|1|1|1|6|391|276
Chr8 exon 49961343 49961449 49961343 ID=MsG0880044838.01.T01:exon:20889;Parent=MsG0880044838.01.T01
Chr8 exon 49961546 49961613 49961546 ID=MsG0880044838.01.T01:exon:20890;Parent=MsG0880044838.01.T01
Chr8 exon 49962217 49962327 49962217 ID=MsG0880044838.01.T01:exon:20891;Parent=MsG0880044838.01.T01
Chr8 exon 49963244 49963330 49963244 ID=MsG0880044838.01.T01:exon:20892;Parent=MsG0880044838.01.T01
Chr8 exon 49963480 49963688 49963480 ID=MsG0880044838.01.T01:exon:20893;Parent=MsG0880044838.01.T01
Chr8 exon 49963820 49964517 49963820 ID=MsG0880044838.01.T01:exon:20894;Parent=MsG0880044838.01.T01
Chr8 five_prime_UTR 49961343 49961400 49961343 ID=MsG0880044838.01.T01:five_prime_utr;Parent=MsG0880044838.01.T01
Chr8 CDS 49961401 49961449 49961401 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 CDS 49961546 49961613 49961546 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 CDS 49962217 49962327 49962217 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 CDS 49963244 49963330 49963244 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 CDS 49963480 49963688 49963480 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 CDS 49963820 49964126 49963820 ID=MsG0880044838.01.T01:cds;Parent=MsG0880044838.01.T01
Chr8 three_prime_UTR 49964127 49964517 49964127 ID=MsG0880044838.01.T01:three_prime_utr;Parent=MsG0880044838.01.T01
Gene Sequence

>MsG0880044838.01.T01

ATGGTGAAGGGTCCTGGTCTTTACTCCGATATCGGCAAGAGAGCTCGAGATCTTCTTTTCAAAGATTACCAGAATGATCACAAGTTCACCATCACAACTCAAACTTTATCTGGAGTGGAAATTACTTCAGCTGGAGTCAGGAAAGGTGAGGCATTTTTGGCTGATGTGAGTACCAAGGTGAAGAACAAGAACGTCACTACTGATATCAAAGTCGACACGAACTCAAATCTGCGCACAACCATTACTGTGGATGAAACAGTGCCTGGACTCAAGACAATTTTTAGCTTCATTTATCCAGAGCAAAAATCTGGCAAGGTGGAACTACAGTACCTTCATGATTATGCTGGGATCAGTACAAGTATTGGGTTGACAGCAACTCCCACTGTTAACATCTCTGCTGTGCTTGGAAATAATTTGGTTTCTGTTGGATCAGATGTTTCCTTTGAGACTTCTTCTGGCTCCTTGAACAAGTGCAATTTTGGGTTGAATGTTACTCATGCTGACCTTATTGCTTCTCTGACTGTCAATGATAGAGGTGATTCCCTCAATGCCTCGTATTACCATGTTGTGAGCCCACTGACAAACACTGCTGTTGGGGCGGAGCTTTCACACACCTTTTCCAGCAATGAGAACGTGCTAACCATTGGCACACAACATGCTCTTGACCCTGTAACCTTGTTGAAAGCTAAGGTGAACAATTATGGTAAGGCAAGTGCTCTGATCCAGCATGACTGGAGCCGACAAGCACGTTTCTCTCTTGTTGGGGAAGTTGATACTGCTGCCATTCAGAAGACTGCAAAGGTTGGTCTTGCTTGTGCCTTGAAGCCTTAG
Protein sequence

>MsG0880044838.01.T01

MVKGPGLYSDIGKRARDLLFKDYQNDHKFTITTQTLSGVEITSAGVRKGEAFLADVSTKVKNKNVTTDIKVDTNSNLRTTITVDETVPGLKTIFSFIYPEQKSGKVELQYLHDYAGISTSIGLTATPTVNISAVLGNNLVSVGSDVSFETSSGSLNKCNFGLNVTHADLIASLTVNDRGDSLNASYYHVVSPLTNTAVGAELSHTFSSNENVLTIGTQHALDPVTLLKAKVNNYGKASALIQHDWSRQARFSLVGEVDTAAIQKTAKVGLACALKP*