Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044946.01.T01 | XP_013445904.1 | 93.548 | 124 | 8 | 0 | 20 | 143 | 553 | 676 | 6.53E-73 | 248 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044946.01.T01 | O48686 | 75.61 | 123 | 30 | 0 | 20 | 142 | 492 | 614 | 1.13E-61 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044946.01.T01 | A0A072U2E6 | 93.548 | 124 | 8 | 0 | 20 | 143 | 553 | 676 | 3.12e-73 | 248 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880044946.01 | MsG0880044948.01 | 0.820687 | 6.013718e-53 | 4.791007e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044946.01.T01 | MTR_8g062700 | 93.548 | 124 | 8 | 0 | 20 | 143 | 553 | 676 | 7.91e-77 | 248 |
| MsG0880044946.01.T01 | MTR_8g062700 | 93.548 | 124 | 8 | 0 | 20 | 143 | 554 | 677 | 8.15e-77 | 248 |
| MsG0880044946.01.T01 | MTR_5g036670 | 73.387 | 124 | 33 | 0 | 20 | 143 | 550 | 673 | 9.19e-61 | 202 |
| MsG0880044946.01.T01 | MTR_6g008970 | 65.041 | 123 | 43 | 0 | 20 | 142 | 536 | 658 | 1.23e-53 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044946.01.T01 | AT1G24190 | 75.610 | 123 | 30 | 0 | 20 | 142 | 488 | 610 | 1.13e-62 | 207 |
| MsG0880044946.01.T01 | AT1G24190 | 75.610 | 123 | 30 | 0 | 20 | 142 | 492 | 614 | 1.15e-62 | 207 |
| MsG0880044946.01.T01 | AT1G24190 | 75.610 | 123 | 30 | 0 | 20 | 142 | 488 | 610 | 1.21e-62 | 207 |
| MsG0880044946.01.T01 | AT1G70060 | 73.171 | 123 | 33 | 0 | 20 | 142 | 506 | 628 | 1.77e-59 | 198 |
| MsG0880044946.01.T01 | AT1G70060 | 73.171 | 123 | 33 | 0 | 20 | 142 | 506 | 628 | 1.77e-59 | 198 |
| MsG0880044946.01.T01 | AT1G59890 | 74.797 | 123 | 31 | 0 | 20 | 142 | 377 | 499 | 7.18e-59 | 196 |
| MsG0880044946.01.T01 | AT1G59890 | 74.797 | 123 | 31 | 0 | 20 | 142 | 381 | 503 | 7.36e-59 | 196 |
| MsG0880044946.01.T01 | AT1G59890 | 74.797 | 123 | 31 | 0 | 20 | 142 | 381 | 503 | 7.41e-59 | 196 |
| MsG0880044946.01.T01 | AT1G59890 | 74.797 | 123 | 31 | 0 | 20 | 142 | 386 | 508 | 7.43e-59 | 196 |
| MsG0880044946.01.T01 | AT1G10450 | 69.919 | 123 | 37 | 0 | 20 | 142 | 364 | 486 | 2.40e-55 | 186 |
| MsG0880044946.01.T01 | AT1G10450 | 69.919 | 123 | 37 | 0 | 20 | 142 | 364 | 486 | 2.40e-55 | 186 |
| MsG0880044946.01.T01 | AT1G10450 | 69.919 | 123 | 37 | 0 | 20 | 142 | 415 | 537 | 2.70e-55 | 186 |
| MsG0880044946.01.T01 | AT5G15020 | 66.667 | 123 | 41 | 0 | 20 | 142 | 528 | 650 | 6.66e-54 | 182 |
| MsG0880044946.01.T01 | AT5G15020 | 66.667 | 123 | 41 | 0 | 20 | 142 | 528 | 650 | 7.13e-54 | 182 |
| MsG0880044946.01.T01 | AT3G01320 | 63.415 | 123 | 45 | 0 | 20 | 142 | 533 | 655 | 1.46e-50 | 172 |
| MsG0880044946.01.T01 | AT3G01320 | 63.415 | 123 | 45 | 0 | 20 | 142 | 521 | 643 | 1.75e-50 | 172 |
Find 31 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATAAATTAAATTAAA+AGG | 0.229569 | 8:-51910330 | None:intergenic |
| CGTATTGAGGAGCATTTATC+AGG | 0.277753 | 8:+51910578 | MsG0880044946.01.T01:CDS |
| GTGCAGAGTTTAATATAGTT+TGG | 0.279806 | 8:+51910859 | MsG0880044946.01.T01:CDS |
| GAATGCATCTCGAGCCTTGC+CGG | 0.335283 | 8:+51910788 | MsG0880044946.01.T01:CDS |
| TGCAGAGTTTAATATAGTTT+GGG | 0.348281 | 8:+51910860 | MsG0880044946.01.T01:CDS |
| ACTCCTCCTGTTTCTGCTTC+AGG | 0.353246 | 8:-51910824 | None:intergenic |
| GTTAATGATGATGTAATTCC+AGG | 0.355314 | 8:+51910542 | MsG0880044946.01.T01:CDS |
| ATGTCTTTGTGAATTCCATT+AGG | 0.362988 | 8:+51910153 | MsG0880044946.01.T01:five_prime_UTR |
| AAATGCTCCTCAATACGAAT+TGG | 0.386991 | 8:-51910572 | None:intergenic |
| AACCCGCCTGAAGCAGAAAC+AGG | 0.442327 | 8:+51910818 | MsG0880044946.01.T01:CDS |
| TCTGTGTTGTAATCAGGTTT+TGG | 0.444903 | 8:+51910112 | None:intergenic |
| AGAAAACAGATCTAACCTAA+TGG | 0.445038 | 8:-51910168 | None:intergenic |
| AGGTGCATTGAACGACTATA+CGG | 0.453204 | 8:+51910729 | MsG0880044946.01.T01:CDS |
| ATACGAATTGGAATATCTCC+TGG | 0.466439 | 8:-51910560 | None:intergenic |
| TGTTTAGATGCGAAGACGAC+AGG | 0.486181 | 8:+51910267 | MsG0880044946.01.T01:five_prime_UTR |
| AGATATTCCAATTCGTATTG+AGG | 0.495294 | 8:+51910565 | MsG0880044946.01.T01:CDS |
| CTCCTGTTTCTGCTTCAGGC+GGG | 0.509322 | 8:-51910820 | None:intergenic |
| TTCAGGCGGGTTAATATCAC+CGG | 0.529652 | 8:-51910807 | None:intergenic |
| CCTCCTGTTTCTGCTTCAGG+CGG | 0.534175 | 8:-51910821 | None:intergenic |
| CATCACATGGTTGATTTGAG+AGG | 0.538053 | 8:+51910407 | MsG0880044946.01.T01:exon |
| TGACCATGGGCTTGATGTGA+TGG | 0.544947 | 8:+51910752 | MsG0880044946.01.T01:CDS |
| GAACGACTATACGGTGACCA+TGG | 0.550770 | 8:+51910738 | MsG0880044946.01.T01:CDS |
| AACGACTATACGGTGACCAT+GGG | 0.556588 | 8:+51910739 | MsG0880044946.01.T01:CDS |
| GAAGCAGAAACAGGAGGAGT+GGG | 0.580158 | 8:+51910827 | MsG0880044946.01.T01:CDS |
| AAACTATATTAAACTCTGCA+CGG | 0.581815 | 8:-51910857 | None:intergenic |
| TGAAGCAGAAACAGGAGGAG+TGG | 0.583162 | 8:+51910826 | MsG0880044946.01.T01:CDS |
| TGCAACAAGTAAGAAAGTTG+AGG | 0.611189 | 8:+51910505 | MsG0880044946.01.T01:CDS |
| GCGGGTTAATATCACCGGCA+AGG | 0.642210 | 8:-51910802 | None:intergenic |
| CTTCCATCACATCAAGCCCA+TGG | 0.664006 | 8:-51910755 | None:intergenic |
| AATTCACAAAGACATCAAAG+AGG | 0.692445 | 8:-51910145 | None:intergenic |
| CCGCCTGAAGCAGAAACAGG+AGG | 0.700575 | 8:+51910821 | MsG0880044946.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAAAAAAAAATATTA+TGG | - | Chr8:51910441-51910460 | None:intergenic | 0.0% |
| !!! | AAATTAAAAGGTATTTTTTA+AGG | - | Chr8:51910321-51910340 | None:intergenic | 10.0% |
| !!! | AATTAAAAGGTATTTTTTAA+GGG | - | Chr8:51910320-51910339 | None:intergenic | 10.0% |
| !! | AATTTATCATAGCATATAAA+TGG | + | Chr8:51910628-51910647 | MsG0880044946.01.T01:intron | 15.0% |
| !!! | TTAATTTATTTTGTATTGGT+TGG | + | Chr8:51910339-51910358 | MsG0880044946.01.T01:five_prime_UTR | 15.0% |
| ! | AAACTATATTAAACTCTGCA+CGG | - | Chr8:51910860-51910879 | None:intergenic | 25.0% |
| ! | TGCAGAGTTTAATATAGTTT+GGG | + | Chr8:51910860-51910879 | MsG0880044946.01.T01:CDS | 25.0% |
| !!! | TTCTACTTTTTGGTTAGTTT+TGG | + | Chr8:51910384-51910403 | MsG0880044946.01.T01:five_prime_UTR | 25.0% |
| !!! | TTGTTTTAGCTCTAAATCTT+AGG | + | Chr8:51910709-51910728 | MsG0880044946.01.T01:intron | 25.0% |
| AATTCACAAAGACATCAAAG+AGG | - | Chr8:51910148-51910167 | None:intergenic | 30.0% | |
| AGAAAACAGATCTAACCTAA+TGG | - | Chr8:51910171-51910190 | None:intergenic | 30.0% | |
| AGAACAGAACACAAAATATC+AGG | - | Chr8:51910669-51910688 | None:intergenic | 30.0% | |
| AGATATTCCAATTCGTATTG+AGG | + | Chr8:51910565-51910584 | MsG0880044946.01.T01:CDS | 30.0% | |
| ATGTCTTTGTGAATTCCATT+AGG | + | Chr8:51910153-51910172 | MsG0880044946.01.T01:five_prime_UTR | 30.0% | |
| GTGCAGAGTTTAATATAGTT+TGG | + | Chr8:51910859-51910878 | MsG0880044946.01.T01:CDS | 30.0% | |
| ! | ATTAGGTTAGATCTGTTTTC+TGG | + | Chr8:51910170-51910189 | MsG0880044946.01.T01:five_prime_UTR | 30.0% |
| ! | ATTTTTGCGCATGTGTTTAA+AGG | - | Chr8:51910231-51910250 | None:intergenic | 30.0% |
| ! | GTTAATGATGATGTAATTCC+AGG | + | Chr8:51910542-51910561 | MsG0880044946.01.T01:CDS | 30.0% |
| !!! | TTTCTGGTTGTTACTTTTTC+TGG | + | Chr8:51910186-51910205 | MsG0880044946.01.T01:five_prime_UTR | 30.0% |
| AAATGCTCCTCAATACGAAT+TGG | - | Chr8:51910575-51910594 | None:intergenic | 35.0% | |
| ATACGAATTGGAATATCTCC+TGG | - | Chr8:51910563-51910582 | None:intergenic | 35.0% | |
| CTGTTCTGATACTATCTTCT+TGG | + | Chr8:51910682-51910701 | MsG0880044946.01.T01:intron | 35.0% | |
| TAACCAAAAAGTAGAACACG+TGG | - | Chr8:51910380-51910399 | None:intergenic | 35.0% | |
| TGCAACAAGTAAGAAAGTTG+AGG | + | Chr8:51910505-51910524 | MsG0880044946.01.T01:CDS | 35.0% | |
| ! | AAGCTTTTACTTTAAGCAGC+AGG | + | Chr8:51910926-51910945 | MsG0880044946.01.T01:CDS | 35.0% |
| AGGTGCATTGAACGACTATA+CGG | + | Chr8:51910729-51910748 | MsG0880044946.01.T01:CDS | 40.0% | |
| !! | CATCACATGGTTGATTTGAG+AGG | + | Chr8:51910407-51910426 | MsG0880044946.01.T01:exon | 40.0% |
| !! | CGTATTGAGGAGCATTTATC+AGG | + | Chr8:51910578-51910597 | MsG0880044946.01.T01:CDS | 40.0% |
| !! | TTGCCACGTGTTCTACTTTT+TGG | + | Chr8:51910374-51910393 | MsG0880044946.01.T01:five_prime_UTR | 40.0% |
| !!! | TGGTTAGTTTTGGCATCACA+TGG | + | Chr8:51910394-51910413 | MsG0880044946.01.T01:exon | 40.0% |
| AACGACTATACGGTGACCAT+GGG | + | Chr8:51910739-51910758 | MsG0880044946.01.T01:CDS | 45.0% | |
| TGTTTAGATGCGAAGACGAC+AGG | + | Chr8:51910267-51910286 | MsG0880044946.01.T01:five_prime_UTR | 45.0% | |
| !! | CTTTTTCTGGTCAGCAAGTG+AGG | + | Chr8:51910199-51910218 | MsG0880044946.01.T01:five_prime_UTR | 45.0% |
| !! | TTCAGGCGGGTTAATATCAC+CGG | - | Chr8:51910810-51910829 | None:intergenic | 45.0% |
| !! | ACAAAATAAATTAAATTAAA+AGG | - | Chr8:51910333-51910352 | None:intergenic | 5.0% |
| !!! | TAATTTAATTTATTTTGTAT+TGG | + | Chr8:51910335-51910354 | MsG0880044946.01.T01:five_prime_UTR | 5.0% |
| !!! | TTTTTTTATTTTATACTTTT+AGG | + | Chr8:51910450-51910469 | MsG0880044946.01.T01:CDS | 5.0% |
| CTTCCATCACATCAAGCCCA+TGG | - | Chr8:51910758-51910777 | None:intergenic | 50.0% | |
| GAACGACTATACGGTGACCA+TGG | + | Chr8:51910738-51910757 | MsG0880044946.01.T01:CDS | 50.0% | |
| GAAGCAGAAACAGGAGGAGT+GGG | + | Chr8:51910827-51910846 | MsG0880044946.01.T01:CDS | 50.0% | |
| TGAAGCAGAAACAGGAGGAG+TGG | + | Chr8:51910826-51910845 | MsG0880044946.01.T01:CDS | 50.0% | |
| TGACCATGGGCTTGATGTGA+TGG | + | Chr8:51910752-51910771 | MsG0880044946.01.T01:CDS | 50.0% | |
| !! | ACTCCTCCTGTTTCTGCTTC+AGG | - | Chr8:51910827-51910846 | None:intergenic | 50.0% |
| AACCCGCCTGAAGCAGAAAC+AGG | + | Chr8:51910818-51910837 | MsG0880044946.01.T01:CDS | 55.0% | |
| GAATGCATCTCGAGCCTTGC+CGG | + | Chr8:51910788-51910807 | MsG0880044946.01.T01:CDS | 55.0% | |
| ! | GCGGGTTAATATCACCGGCA+AGG | - | Chr8:51910805-51910824 | None:intergenic | 55.0% |
| !! | CCTCCTGTTTCTGCTTCAGG+CGG | - | Chr8:51910824-51910843 | None:intergenic | 55.0% |
| !! | CTCCTGTTTCTGCTTCAGGC+GGG | - | Chr8:51910823-51910842 | None:intergenic | 55.0% |
| CCGCCTGAAGCAGAAACAGG+AGG | + | Chr8:51910821-51910840 | MsG0880044946.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 51910122 | 51910962 | 51910122 | ID=MsG0880044946.01;Name=MsG0880044946.01 |
| Chr8 | mRNA | 51910122 | 51910962 | 51910122 | ID=MsG0880044946.01.T01;Parent=MsG0880044946.01;Name=MsG0880044946.01.T01;_AED=0.47;_eAED=0.47;_QI=291|1|1|1|0.66|0.5|4|0|143 |
| Chr8 | exon | 51910122 | 51910599 | 51910122 | ID=MsG0880044946.01.T01:exon:22140;Parent=MsG0880044946.01.T01 |
| Chr8 | exon | 51910718 | 51910962 | 51910718 | ID=MsG0880044946.01.T01:exon:22141;Parent=MsG0880044946.01.T01 |
| Chr8 | five_prime_UTR | 51910122 | 51910412 | 51910122 | ID=MsG0880044946.01.T01:five_prime_utr;Parent=MsG0880044946.01.T01 |
| Chr8 | CDS | 51910413 | 51910599 | 51910413 | ID=MsG0880044946.01.T01:cds;Parent=MsG0880044946.01.T01 |
| Chr8 | CDS | 51910718 | 51910962 | 51910718 | ID=MsG0880044946.01.T01:cds;Parent=MsG0880044946.01.T01 |
| Gene Sequence |
| Protein sequence |
