AlfalfaGEDB | MsG0880044948.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044948.01

KEGG: K11644
GO: -
PFAM: PF08295

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044948.01.T01	XP_013445904.1	95.139	144	7	0	1	144	537	680	7.39E-85	293

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044948.01.T01	O48686	77.083	144	33	0	1	144	476	619	7.33E-72	248

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044948.01.T01	A0A072U2E6	95.139	144	7	0	1	144	537	680	3.53e-85	293

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047958.01	MsG0880044948.01	0.802477	5.506871e-49	2.700200e-46
MsG0080048672.01	MsG0880044948.01	0.825818	3.809674e-54	3.510353e-51
MsG0080048769.01	MsG0880044948.01	0.805317	1.415197e-49	7.463819e-47
MsG0080048787.01	MsG0880044948.01	0.824844	6.475555e-54	5.803566e-51
MsG0180000091.01	MsG0880044948.01	0.814586	1.429083e-51	9.627394e-49
MsG0180000114.01	MsG0880044948.01	0.829328	5.473376e-55	5.584717e-52
MsG0180000144.01	MsG0880044948.01	0.810706	1.008638e-50	6.123809e-48
MsG0180000146.01	MsG0880044948.01	0.810571	1.078675e-50	6.525076e-48
MsG0180000702.01	MsG0880044948.01	0.819995	8.664074e-53	6.771969e-50
MsG0180000703.01	MsG0880044948.01	0.806783	6.955805e-50	3.808904e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044948.01.T01	MTR_8g062700	95.139	144	7	0	1	144	537	680	8.95e-89	293
MsG0880044948.01.T01	MTR_8g062700	95.139	144	7	0	1	144	538	681	8.98e-89	293
MsG0880044948.01.T01	MTR_5g036670	77.778	144	32	0	1	144	534	677	6.04e-73	248
MsG0880044948.01.T01	MTR_6g008970	68.750	144	45	0	1	144	520	663	2.32e-65	226
MsG0880044948.01.T01	MTR_0202s0040	37.627	295	148	5	146	411	258	545	7.63e-48	171
MsG0880044948.01.T01	MTR_3g048220	35.932	295	153	5	146	411	256	543	1.29e-43	160
MsG0880044948.01.T01	MTR_6g090350	34.247	292	162	4	146	411	275	562	1.24e-39	149
MsG0880044948.01.T01	MTR_0139s0020	32.646	291	134	3	146	411	372	625	4.84e-38	145
MsG0880044948.01.T01	MTR_6g464440	34.296	277	160	8	146	411	383	648	1.10e-32	130
MsG0880044948.01.T01	MTR_6g034305	35.398	226	99	6	127	325	250	455	1.12e-24	105

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044948.01.T01	AT1G24190	77.083	144	33	1	144	472	615	6.80e-73	248
MsG0880044948.01.T01	AT1G24190	77.083	144	33	1	144	472	615	7.15e-73	248
MsG0880044948.01.T01	AT1G24190	77.083	144	33	1	144	476	619	7.48e-73	248
MsG0880044948.01.T01	AT1G59890	77.778	144	32	1	144	361	504	3.63e-71	243
MsG0880044948.01.T01	AT1G59890	77.778	144	32	1	144	370	513	3.70e-71	243
MsG0880044948.01.T01	AT1G59890	77.778	144	32	1	144	365	508	3.77e-71	243
MsG0880044948.01.T01	AT1G59890	77.778	144	32	1	144	365	508	3.94e-71	243
MsG0880044948.01.T01	AT1G70060	75.694	144	35	1	144	490	633	4.14e-71	243
MsG0880044948.01.T01	AT1G70060	75.694	144	35	1	144	490	633	4.14e-71	243
MsG0880044948.01.T01	AT1G10450	73.611	144	38	1	144	348	491	1.71e-67	232

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Find 107 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTATATTAAACTCTGCA+CGG	0.581815	8:-51918848	None:intergenic
AAATGCTCCTCAATACGAAT+TGG	0.386991	8:-51918564	None:intergenic
AAATGTTCAGCTGGAAGAAG+AGG	0.549268	8:+51920154	MsG0880044948.01.T01:CDS
AACCACTTCTTCGAATGATA+AGG	0.500316	8:+51919664	MsG0880044948.01.T01:CDS
AACCCGCCTGAAGCAGAAAC+AGG	0.442327	8:+51918809	MsG0880044948.01.T01:CDS
AACGACTATACGGTGACCAT+GGG	0.556588	8:+51918730	MsG0880044948.01.T01:CDS
AACGGGGCTCTGAAGTCCAA+AGG	0.620936	8:+51920032	MsG0880044948.01.T01:CDS
AACTACTGAGAAAGAAGCCG+TGG	0.606344	8:+51920103	MsG0880044948.01.T01:CDS
AACTGGTAAGCGTAATAATG+TGG	0.559507	8:+51919616	MsG0880044948.01.T01:CDS
AAGAAGCCGTGGAGCTGGAG+AGG	0.445524	8:+51920114	MsG0880044948.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAACAGAACACAAAATATC+AGG	-	Chr8:51918661-51918680	None:intergenic	30.0%
AGATATTCCAATTCGTATTG+AGG	+	Chr8:51918557-51918576	MsG0880044948.01.T01:CDS	30.0%
CATAAACTCTTGAATAAGGA+AGG	+	Chr8:51919235-51919254	MsG0880044948.01.T01:intron	30.0%
CTTCCTTATTCAAGAGTTTA+TGG	-	Chr8:51919237-51919256	None:intergenic	30.0%
GATTCTCCTATAAGAAAGAA+AGG	+	Chr8:51919590-51919609	MsG0880044948.01.T01:CDS	30.0%
GTGCAGAGTTTAATATAGTT+TGG	+	Chr8:51918850-51918869	MsG0880044948.01.T01:CDS	30.0%
TAAACTCTTGAATAAGGAAG+GGG	+	Chr8:51919237-51919256	MsG0880044948.01.T01:intron	30.0%
TGTTTAGAAACCAGATTGAA+CGG	+	Chr8:51920014-51920033	MsG0880044948.01.T01:CDS	30.0%
AAATGCTCCTCAATACGAAT+TGG	-	Chr8:51918567-51918586	None:intergenic	35.0%
AACCACTTCTTCGAATGATA+AGG	+	Chr8:51919664-51919683	MsG0880044948.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	51918233	51920328	51918233	ID=MsG0880044948.01;Name=MsG0880044948.01
Chr8	mRNA	51918233	51920328	51918233	ID=MsG0880044948.01.T01;Parent=MsG0880044948.01;Name=MsG0880044948.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|0.4\|0.33\|1\|1\|1\|6\|0\|412
Chr8	exon	51918233	51918282	51918233	ID=MsG0880044948.01.T01:exon:22144;Parent=MsG0880044948.01.T01
Chr8	exon	51918464	51918591	51918464	ID=MsG0880044948.01.T01:exon:22145;Parent=MsG0880044948.01.T01
Chr8	exon	51918709	51918963	51918709	ID=MsG0880044948.01.T01:exon:22146;Parent=MsG0880044948.01.T01
Chr8	exon	51919366	51919726	51919366	ID=MsG0880044948.01.T01:exon:22147;Parent=MsG0880044948.01.T01
Chr8	exon	51919808	51919880	51919808	ID=MsG0880044948.01.T01:exon:22148;Parent=MsG0880044948.01.T01
Chr8	exon	51919957	51920328	51919957	ID=MsG0880044948.01.T01:exon:22149;Parent=MsG0880044948.01.T01
Chr8	CDS	51918233	51918282	51918233	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01
Chr8	CDS	51918464	51918591	51918464	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01
Chr8	CDS	51918709	51918963	51918709	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01
Chr8	CDS	51919366	51919726	51919366	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01
Chr8	CDS	51919808	51919880	51919808	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01
Chr8	CDS	51919957	51920328	51919957	ID=MsG0880044948.01.T01:cds;Parent=MsG0880044948.01.T01

Gene Sequence

>MsG0880044948.01.T01

ATGCGCAAAAATCAGTATGAAGAGAGCTTGTTTAGATGCGAAGACGACAGGTTTGAACTTGATATGTTGTTAGAGTCTATAAATGCAACAAGTAAGAAAGTTGAGGAGATCATAGAAAAAGTTAATGATGATGTAATTCCAGGAGATATTCCAATTCGTATTGAGGAGCATTTATCAGCTCTAAATCTTAGGTGCATTGAACGACTATACGGTGACCATGGGCTTGATGTGATGGAAGTGTTGAAAAAGAATGCATCTCGAGCCTTGCCGGTGATATTAACCCGCCTGAAGCAGAAACAGGAGGAGTGGGCAAGATGCCGTGCAGAGTTTAATATAGTTTGGGCTGATGTATATGCGAAGAATTATCATAAATCACTTGATCATCGAAGCTTTTACTTTAAGCAGCAGGATACAAAAAGCTTGAGCACTAAAGAACCAATGTTGCCCATGACGCCTTCTGAGAGAAACAAATACTTGGCTGAAGTGAAGGTGAGGAAGGCAGCCGAGGGATATGCTACCAGTGATCCCGCCGGTGTAACTCATCGTAAAGCAAAGAGAAAAGACATTATTTCAAAGGATGATGCTGTTACTGTTCAGGTTGATACGGAGCTTGAGAACGTCGAGCAGGTTGTCGACCGGACTGGAGCGGAGATTGTGGATTCTCCTATAAGAAAGAAAGGGAGAACTGGTAAGCGTAATAATGTGGGTGATAAGAGCGTCCATACCGCTGCAACCACTTCTTCGAATGATAAGGTGGGGGAGTCTTTTTGGCACAAAGAGTTTAATTTTCGAAGGTATATGGAGGAAAATGTTCCCCTGGCGGATGTTGACGTTGATGCGGGAAGTAAAAGTAAGTTTGGCGACTTGAGAAAAAAATATAAAGATGCTTCTGAAGATGTTGAGAAATGGCGACACAAGGCAACATGTTTAGAAACCAGATTGAACGGGGCTCTGAAGTCCAAAGGTAAGGCTGAGGAAATTGCGAAGAGTTCTGAGGAAAAACTGGCGACTTTAACTACTGAGAAAGAAGCCGTGGAGCTGGAGAGGGATTCTTTGAAAGAAAGAAATGTTCAGCTGGAAGAAGAGGTGAAGGAGTGCAAAGGGGCCCTGGCAAAATATTTTGATGACGGCTTTGAGCGCGCCCGTGAACAGGCTAAATGGTTCTATCCTGAGGCAGACTTTAGTGGCCTGGATGCTTTTAAGATTGTCGAAGATGGGAAGTTGGTCGATGAAGAATGA

Protein sequence

>MsG0880044948.01.T01

MRKNQYEESLFRCEDDRFELDMLLESINATSKKVEEIIEKVNDDVIPGDIPIRIEEHLSALNLRCIERLYGDHGLDVMEVLKKNASRALPVILTRLKQKQEEWARCRAEFNIVWADVYAKNYHKSLDHRSFYFKQQDTKSLSTKEPMLPMTPSERNKYLAEVKVRKAAEGYATSDPAGVTHRKAKRKDIISKDDAVTVQVDTELENVEQVVDRTGAEIVDSPIRKKGRTGKRNNVGDKSVHTAATTSSNDKVGESFWHKEFNFRRYMEENVPLADVDVDAGSKSKFGDLRKKYKDASEDVEKWRHKATCLETRLNGALKSKGKAEEIAKSSEEKLATLTTEKEAVELERDSLKERNVQLEEEVKECKGALAKYFDDGFERAREQAKWFYPEADFSGLDAFKIVEDGKLVDEE*