AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045123.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045123.01.T01 MTR_4g037355 40.441 136 61 4 140 275 1 116 3.33e-22 90.9
MsG0880045123.01.T01 MTR_4g112730 57.971 69 28 1 128 196 2 69 4.25e-18 78.6
MsG0880045123.01.T01 MTR_7g062720 44.000 75 42 0 57 131 68 142 5.65e-17 77.8
MsG0880045123.01.T01 MTR_6g088150 74.419 43 11 0 14 56 14 56 1.72e-16 73.9
MsG0880045123.01.T01 MTR_0007s0410 75.556 45 11 0 12 56 183 227 1.37e-15 75.5
MsG0880045123.01.T01 MTR_5g061150 39.241 79 48 0 57 135 58 136 1.40e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATCAATGGAACAAATTA+AGG 0.196151 8:-54561617 MsG0880045123.01.T01:CDS
AGTCTTATTGCCCTCATTAA+TGG 0.201947 8:-54561637 MsG0880045123.01.T01:CDS
AAAGTTGTGGCGTTTATTTC+AGG 0.203868 8:+54561321 None:intergenic
GACAAAATTAAGGCAAGATT+TGG 0.257188 8:-54561672 MsG0880045123.01.T01:CDS
AAGACAGGTAGTATCATTAT+TGG 0.259043 8:+54560948 None:intergenic
CACGGCCTCCACCACGAAAA+TGG 0.277262 8:+54561205 None:intergenic
CGTGGTGGCTATTCTCGTTT+TGG 0.291404 8:-54561261 MsG0880045123.01.T01:CDS
CATAGGAAAAGATATTAATA+TGG 0.302496 8:-54560911 MsG0880045123.01.T01:CDS
ATTTACTGAATACTCTGATT+TGG 0.313227 8:-54561427 MsG0880045123.01.T01:CDS
GATGGAAGTTGTGGTGCTTC+TGG 0.324672 8:-54564757 MsG0880045123.01.T01:CDS
CTTGATGATTCTTGTTGGTT+TGG 0.339105 8:+54561145 None:intergenic
GAAGAGGTTATATGAATTAT+AGG 0.339571 8:-54561182 MsG0880045123.01.T01:CDS
TTTACCTTGATGATTCTTGT+TGG 0.362649 8:+54561140 None:intergenic
AATAGCCACCACGTCCTCTT+TGG 0.369504 8:+54561271 None:intergenic
CTTGAGGCTGCTGATTTCAT+AGG 0.370971 8:-54560928 MsG0880045123.01.T01:CDS
AGCGAATGTTAACAGAAATT+AGG 0.384603 8:+54564854 None:intergenic
TGCGTTAAGTTGAATATTGA+TGG 0.392837 8:-54564775 MsG0880045123.01.T01:CDS
AGACAGGTAGTATCATTATT+GGG 0.395290 8:+54560949 None:intergenic
ACAAAATTAAGGCAAGATTT+GGG 0.416566 8:-54561671 MsG0880045123.01.T01:CDS
TATAACTGAAAAGGAAAAGT+TGG 0.422757 8:-54561514 MsG0880045123.01.T01:CDS
CGTGGTGGAGGCCGTGGAAG+AGG 0.431707 8:-54561198 MsG0880045123.01.T01:CDS
TGCCCTCATTAATGGATCAA+TGG 0.432677 8:-54561629 MsG0880045123.01.T01:CDS
TTTATAGACATCATGCATAC+TGG 0.445291 8:-54564877 None:intergenic
TGGCAAAACTATAACTGAAA+AGG 0.467052 8:-54561523 MsG0880045123.01.T01:CDS
AACCACCAGTTGCGTCCTAC+AGG 0.478404 8:-54561357 MsG0880045123.01.T01:CDS
TTGTTCCTGTAGGACGCAAC+TGG 0.480977 8:+54561352 None:intergenic
TTAATCGTAACATTGTTAAG+TGG 0.490263 8:-54564816 MsG0880045123.01.T01:CDS
TGCAACAACAATATAGAATG+AGG 0.496917 8:-54561452 MsG0880045123.01.T01:CDS
ATCTGTTGAAGGAACGGAAA+AGG 0.496988 8:-54560995 MsG0880045123.01.T01:CDS
TCTGGTGACATTGGCTCTAG+AGG 0.503730 8:-54564739 MsG0880045123.01.T01:CDS
AACAAGAATCATCAAGGTAA+AGG 0.504207 8:-54561138 MsG0880045123.01.T01:CDS
TGTGGTGCTTCTGGTGACAT+TGG 0.504480 8:-54564748 MsG0880045123.01.T01:CDS
GTTCCATTGATCCATTAATG+AGG 0.509644 8:+54561626 None:intergenic
TGTTATAGATGCGGAAAGAA+AGG 0.513070 8:-54561072 MsG0880045123.01.T01:CDS
AGATACAAAGAATCTGTTGA+AGG 0.515186 8:-54561006 MsG0880045123.01.T01:CDS
AGGTTTACTTCGTGAGAACA+AGG 0.532650 8:-54564719 MsG0880045123.01.T01:CDS
TTCCATTGATCCATTAATGA+GGG 0.533283 8:+54561627 None:intergenic
GATGCGGAAAGAAAGGTCAT+TGG 0.533423 8:-54561065 MsG0880045123.01.T01:CDS
ACGATTAATGTGGTGCTCAA+AGG 0.535148 8:+54564831 None:intergenic
TTGGTTTGGTCATATTTGAG+AGG 0.544297 8:+54561159 None:intergenic
AACTTAACGCATCCATCTTG+AGG 0.552695 8:+54564787 None:intergenic
CGTGGTCGAGGCAGAAGTCA+TGG 0.554869 8:-54561234 MsG0880045123.01.T01:CDS
TTGAATATTGATGGAAGTTG+TGG 0.555331 8:-54564766 MsG0880045123.01.T01:CDS
GAAATCAGCAGCCTCAAGAC+AGG 0.558951 8:+54560933 None:intergenic
CAAAGAATCTGTTGAAGGAA+CGG 0.560333 8:-54561001 MsG0880045123.01.T01:CDS
GGATATGTCATTGTTCCTGT+AGG 0.570307 8:+54561342 None:intergenic
CAAACCAACAAGAATCATCA+AGG 0.574004 8:-54561144 MsG0880045123.01.T01:CDS
TTTGGTTACAGCTCTTCTAG+TGG 0.574259 8:-54561409 MsG0880045123.01.T01:CDS
GCAACAACAATATAGAATGA+GGG 0.579939 8:-54561451 MsG0880045123.01.T01:CDS
TTCCTGTAGGACGCAACTGG+TGG 0.593030 8:+54561355 None:intergenic
AATTCATATAACCTCTTCCA+CGG 0.595635 8:+54561187 None:intergenic
AAGTGGACCTCACCTCAAGA+TGG 0.604795 8:-54564799 MsG0880045123.01.T01:CDS
ACGCCCTTGATTAAAAGTTG+TGG 0.608515 8:+54561308 None:intergenic
GCAATATCACTTGAGATGCA+TGG 0.608661 8:+54561472 None:intergenic
TATAATCGCCAAAGAGGACG+TGG 0.608957 8:-54561279 MsG0880045123.01.T01:CDS
TAACAATGTTACGATTAATG+TGG 0.623689 8:+54564821 None:intergenic
TAATGATACTACCTGTCTTG+AGG 0.625158 8:-54560944 MsG0880045123.01.T01:CDS
ACGATCATCATGATTTCTAG+AGG 0.627654 8:+54561095 None:intergenic
GTCATTGGTCTAAAGCATGT+AGG 0.629444 8:-54561050 MsG0880045123.01.T01:CDS
TGTTGAAGGAACGGAAAAGG+AGG 0.635400 8:-54560992 MsG0880045123.01.T01:CDS
GATGATCGTTGTTATAGATG+CGG 0.637106 8:-54561081 MsG0880045123.01.T01:CDS
CGATCATCATGATTTCTAGA+GGG 0.638172 8:+54561096 None:intergenic
GGTTTACTTCGTGAGAACAA+GGG 0.641003 8:-54564718 MsG0880045123.01.T01:intron
GGTGGTTATAATCGCCAAAG+AGG 0.642086 8:-54561285 MsG0880045123.01.T01:CDS
AACGCATCCATCTTGAGGTG+AGG 0.646251 8:+54564792 None:intergenic
AATCGCCAAAGAGGACGTGG+TGG 0.698905 8:-54561276 MsG0880045123.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTATATTAAAAT+AGG - Chr8:54563227-54563246 MsG0880045123.01.T01:intron 0.0%
!! TAAAATTGAAAAAAAGAATA+AGG + Chr8:54562043-54562062 None:intergenic 10.0%
!! TTTCTTAAAAAATAATCAAA+GGG + Chr8:54562067-54562086 None:intergenic 10.0%
!!! AAAATAAAATAAAGTATTGA+TGG + Chr8:54562133-54562152 None:intergenic 10.0%
!!! ATGAATTAATTTTTCTTAAA+TGG + Chr8:54563012-54563031 None:intergenic 10.0%
!!! ATTTTCACATATTTTATAAT+AGG - Chr8:54563144-54563163 MsG0880045123.01.T01:intron 10.0%
!!! GCTATTAATTTTATAATTTA+GGG - Chr8:54563077-54563096 MsG0880045123.01.T01:intron 10.0%
!!! TGCTATTAATTTTATAATTT+AGG - Chr8:54563076-54563095 MsG0880045123.01.T01:intron 10.0%
!!! TTATGTTTAAAATTTTGAAT+TGG + Chr8:54563871-54563890 None:intergenic 10.0%
!!! TTTTCACATATTTTATAATA+GGG - Chr8:54563145-54563164 MsG0880045123.01.T01:intron 10.0%
!! AGTGCATATTTAATTATTAA+GGG + Chr8:54561810-54561829 None:intergenic 15.0%
!! ATAAATAAATATGGCTTAAT+AGG - Chr8:54561850-54561869 MsG0880045123.01.T01:intron 15.0%
!! ATATTGCATATTATATGTAA+AGG + Chr8:54561623-54561642 None:intergenic 15.0%
!! CAAATGAAAAAAAATATTGT+TGG + Chr8:54562379-54562398 None:intergenic 15.0%
!! CTTTCTTAAAAAATAATCAA+AGG + Chr8:54562068-54562087 None:intergenic 15.0%
!! TAGTGCATATTTAATTATTA+AGG + Chr8:54561811-54561830 None:intergenic 15.0%
!!! AATCTTAAATTCCTAATTTT+CGG + Chr8:54562628-54562647 None:intergenic 15.0%
!!! AATTAATTTTTCTTAAATGG+TGG + Chr8:54563009-54563028 None:intergenic 15.0%
!!! ATATTTTGATTTCTCAAAAA+TGG + Chr8:54563673-54563692 None:intergenic 15.0%
!!! CAACAATATTTTTTTTCATT+TGG - Chr8:54562377-54562396 MsG0880045123.01.T01:intron 15.0%
!!! TTCAAAATTTTAAACATAAC+TGG - Chr8:54563872-54563891 MsG0880045123.01.T01:intron 15.0%
!! AAAAAAAAGTAACTTTGAAC+TGG + Chr8:54561512-54561531 None:intergenic 20.0%
!! AATAATAATCTAACACACTT+CGG + Chr8:54563272-54563291 None:intergenic 20.0%
!! ATGAAAACTGATAATTATTC+AGG + Chr8:54561887-54561906 None:intergenic 20.0%
!! CATAGGAAAAGATATTAATA+TGG - Chr8:54564860-54564879 MsG0880045123.01.T01:CDS 20.0%
!! CATATTATATGTAAAGGTTA+GGG + Chr8:54561617-54561636 None:intergenic 20.0%
!! TATTTAATTATTAAGGGATG+TGG + Chr8:54561804-54561823 None:intergenic 20.0%
!! TGAAAACTGATAATTATTCA+GGG + Chr8:54561886-54561905 None:intergenic 20.0%
!! TTACTAAAGACAATAAAACA+TGG + Chr8:54563125-54563144 None:intergenic 20.0%
!!! AAAATATTGTTGGTTATGAA+AGG + Chr8:54562369-54562388 None:intergenic 20.0%
!!! AAATATTGTTGGTTATGAAA+GGG + Chr8:54562368-54562387 None:intergenic 20.0%
!!! AATCTTAAATCCCTAATTTT+CGG + Chr8:54562714-54562733 None:intergenic 20.0%
!!! AATGGATTTCGATTTTAAAA+GGG + Chr8:54562660-54562679 None:intergenic 20.0%
!!! AGTGTAGATTTTAATGTAAA+AGG - Chr8:54563335-54563354 MsG0880045123.01.T01:intron 20.0%
!!! ATTTATGTTGTTTGTTTTTC+TGG + Chr8:54562239-54562258 None:intergenic 20.0%
!!! GTTTGTTGTTTTCTAAATTT+GGG + Chr8:54562571-54562590 None:intergenic 20.0%
!!! TTTTGTTTTTATGCAACAAA+TGG + Chr8:54562488-54562507 None:intergenic 20.0%
! AATAAGAAACTCTATTCAAC+AGG - Chr8:54561165-54561184 MsG0880045123.01.T01:CDS 25.0%
! ACAAAATTAAGGCAAGATTT+GGG - Chr8:54564100-54564119 MsG0880045123.01.T01:intron 25.0%
! AGACAAATGAAAGAATTGAA+TGG + Chr8:54562830-54562849 None:intergenic 25.0%
! ATATTAATTCTCAGATCTGA+AGG + Chr8:54562951-54562970 None:intergenic 25.0%
! ATTTACTGAATACTCTGATT+TGG - Chr8:54564344-54564363 MsG0880045123.01.T01:intron 25.0%
! CAATCAAAATCCGAAAATTA+GGG - Chr8:54562701-54562720 MsG0880045123.01.T01:intron 25.0%
! GAAAAAAAGAATAAGGTCTA+CGG + Chr8:54562036-54562055 None:intergenic 25.0%
! GAAAACTGATAATTATTCAG+GGG + Chr8:54561885-54561904 None:intergenic 25.0%
! GAAGAGGTTATATGAATTAT+AGG - Chr8:54564589-54564608 MsG0880045123.01.T01:intron 25.0%
! GCATATTATATGTAAAGGTT+AGG + Chr8:54561618-54561637 None:intergenic 25.0%
! GTAATTCAAGCATCTTTAAA+GGG + Chr8:54561693-54561712 None:intergenic 25.0%
! TAACAATGTTACGATTAATG+TGG + Chr8:54560953-54560972 None:intergenic 25.0%
! TAATCAAGAGAAAAAGAAGT+AGG + Chr8:54563701-54563720 None:intergenic 25.0%
! TAATTTCACACATGTAATAG+TGG - Chr8:54563600-54563619 MsG0880045123.01.T01:intron 25.0%
! TATAACTGAAAAGGAAAAGT+TGG - Chr8:54564257-54564276 MsG0880045123.01.T01:intron 25.0%
! TCAATCAAAATCCGAAAATT+AGG - Chr8:54562700-54562719 MsG0880045123.01.T01:intron 25.0%
! TGATTGAAGGAAAAACAATA+CGG + Chr8:54562687-54562706 None:intergenic 25.0%
! TGTAATTCAAGCATCTTTAA+AGG + Chr8:54561694-54561713 None:intergenic 25.0%
! TTAATCGTAACATTGTTAAG+TGG - Chr8:54560955-54560974 MsG0880045123.01.T01:CDS 25.0%
! TTCTAATTTGACATTTACCT+AGG + Chr8:54563568-54563587 None:intergenic 25.0%
!! AAAAGATCCCAAGATTTTAT+GGG - Chr8:54564077-54564096 MsG0880045123.01.T01:intron 25.0%
!! AATTTTGTCCCATAAAATCT+TGG + Chr8:54564088-54564107 None:intergenic 25.0%
!! ACTAATTTAGAATTGAGAAG+AGG + Chr8:54562324-54562343 None:intergenic 25.0%
!! ACTATTGTAAGTTGACAATT+TGG + Chr8:54563526-54563545 None:intergenic 25.0%
!! ATTCTTTGTATCTTTTACAC+AGG + Chr8:54564758-54564777 None:intergenic 25.0%
!! ATTGCACAATTAGAATTTTG+TGG - Chr8:54564228-54564247 MsG0880045123.01.T01:intron 25.0%
!! ATTTTGTCCCATAAAATCTT+GGG + Chr8:54564087-54564106 None:intergenic 25.0%
!! GATTTTATGGGACAAAATTA+AGG - Chr8:54564089-54564108 MsG0880045123.01.T01:intron 25.0%
!! GTCATTCTAAAAAAAGGAAA+AGG - Chr8:54561555-54561574 MsG0880045123.01.T01:CDS 25.0%
!! TCCTTTGTCATTCTAAAAAA+AGG - Chr8:54561549-54561568 MsG0880045123.01.T01:CDS 25.0%
!!! AACATGAGTTTTATTAGCTA+TGG + Chr8:54561917-54561936 None:intergenic 25.0%
!!! AATTTTCGGATTTTGATTGA+AGG + Chr8:54562700-54562719 None:intergenic 25.0%
!!! ACATGAGTTTTATTAGCTAT+GGG + Chr8:54561916-54561935 None:intergenic 25.0%
!!! AGATTTTAATGTAAAAGGAG+AGG - Chr8:54563340-54563359 MsG0880045123.01.T01:intron 25.0%
!!! CAACAACAGTTTTATAATCT+TGG + Chr8:54564182-54564201 None:intergenic 25.0%
!!! CAATGGATTTCGATTTTAAA+AGG + Chr8:54562661-54562680 None:intergenic 25.0%
!!! GATTTTTTTTCTTTGCTGTT+GGG + Chr8:54562791-54562810 None:intergenic 25.0%
!!! GGTTTGTTGTTTTCTAAATT+TGG + Chr8:54562572-54562591 None:intergenic 25.0%
!!! TATACAGTTTTTGGAATAGT+GGG + Chr8:54562594-54562613 None:intergenic 25.0%
!!! TCCTTTTTTTAGAATGACAA+AGG + Chr8:54561553-54561572 None:intergenic 25.0%
!!! TTATACAGTTTTTGGAATAG+TGG + Chr8:54562595-54562614 None:intergenic 25.0%
AAAGGTAAAAACATACAGGA+AGG - Chr8:54564651-54564670 MsG0880045123.01.T01:intron 30.0%
AACAAGAATCATCAAGGTAA+AGG - Chr8:54564633-54564652 MsG0880045123.01.T01:intron 30.0%
AAGACAGGTAGTATCATTAT+TGG + Chr8:54564826-54564845 None:intergenic 30.0%
AATAGACTTGCATGATCTAA+TGG + Chr8:54562095-54562114 None:intergenic 30.0%
AATTCATATAACCTCTTCCA+CGG + Chr8:54564587-54564606 None:intergenic 30.0%
AGAATTGAATGGTACAAATC+TGG + Chr8:54562819-54562838 None:intergenic 30.0%
AGACAGGTAGTATCATTATT+GGG + Chr8:54564825-54564844 None:intergenic 30.0%
AGATACAAAGAATCTGTTGA+AGG - Chr8:54564765-54564784 MsG0880045123.01.T01:CDS 30.0%
AGCGAATGTTAACAGAAATT+AGG + Chr8:54560920-54560939 None:intergenic 30.0%
AGGTAAAGGTAAAAACATAC+AGG - Chr8:54564647-54564666 MsG0880045123.01.T01:intron 30.0%
ATATGGCTTAATAGGTCATT+TGG - Chr8:54561858-54561877 MsG0880045123.01.T01:intron 30.0%
GAAGATGATGAAGAAATTGA+GGG + Chr8:54562184-54562203 None:intergenic 30.0%
GACAAAATTAAGGCAAGATT+TGG - Chr8:54564099-54564118 MsG0880045123.01.T01:intron 30.0%
GCAACAACAATATAGAATGA+GGG - Chr8:54564320-54564339 MsG0880045123.01.T01:intron 30.0%
GTAGTATAAATACCACACTT+TGG - Chr8:54563631-54563650 MsG0880045123.01.T01:intron 30.0%
GTATGTACATAAGAGAGAAA+GGG + Chr8:54561719-54561738 None:intergenic 30.0%
GTGATAATTACCTATTACCT+AGG - Chr8:54563548-54563567 MsG0880045123.01.T01:intron 30.0%
GTTTGTAATCATCATCAATG+TGG + Chr8:54564042-54564061 None:intergenic 30.0%
TCGGAAAAAAATCGTAATGT+GGG + Chr8:54563253-54563272 None:intergenic 30.0%
TGCAACAACAATATAGAATG+AGG - Chr8:54564319-54564338 MsG0880045123.01.T01:intron 30.0%
TGGATCAATGGAACAAATTA+AGG - Chr8:54564154-54564173 MsG0880045123.01.T01:intron 30.0%
TGGCAAAACTATAACTGAAA+AGG - Chr8:54564248-54564267 MsG0880045123.01.T01:intron 30.0%
TGTATGTACATAAGAGAGAA+AGG + Chr8:54561720-54561739 None:intergenic 30.0%
TTATGAAAGGGAAATTGTAG+TGG + Chr8:54562356-54562375 None:intergenic 30.0%
TTCCATTGATCCATTAATGA+GGG + Chr8:54564147-54564166 None:intergenic 30.0%
TTCGGAAAAAAATCGTAATG+TGG + Chr8:54563254-54563273 None:intergenic 30.0%
TTGAATATTGATGGAAGTTG+TGG - Chr8:54561005-54561024 MsG0880045123.01.T01:CDS 30.0%
TTGACATTTACCTAGGTAAT+AGG + Chr8:54563561-54563580 None:intergenic 30.0%
! AATTCCTAATTTTCGGATTC+TGG + Chr8:54562621-54562640 None:intergenic 30.0%
! AATTTAGAATTGAGAAGAGG+AGG + Chr8:54562321-54562340 None:intergenic 30.0%
! ATTCCTAATTTTCGGATTCT+GGG + Chr8:54562620-54562639 None:intergenic 30.0%
! CAAAAGATCCCAAGATTTTA+TGG - Chr8:54564076-54564095 MsG0880045123.01.T01:intron 30.0%
! TGAATGTTGAAACGATCTTT+TGG + Chr8:54562438-54562457 None:intergenic 30.0%
! TGTAATTCAAGCATCGTATA+AGG + Chr8:54561780-54561799 None:intergenic 30.0%
! TTTACCTTGATGATTCTTGT+TGG + Chr8:54564634-54564653 None:intergenic 30.0%
! TTTTTACATGAACCAAAGTG+TGG + Chr8:54563646-54563665 None:intergenic 30.0%
!! AATTTTACTTTTCAAGCGAG+AGG + Chr8:54561745-54561764 None:intergenic 30.0%
!! ATGGTGGAAAGAATGATTTT+AGG + Chr8:54562292-54562311 None:intergenic 30.0%
!! TAGGTTTGAGTTGATAATGA+TGG + Chr8:54562273-54562292 None:intergenic 30.0%
!! TCTGGGTTTTATACAGTTTT+TGG + Chr8:54562603-54562622 None:intergenic 30.0%
!! TGCGTTAAGTTGAATATTGA+TGG - Chr8:54560996-54561015 MsG0880045123.01.T01:CDS 30.0%
!!! ATACAGTTTTTGGAATAGTG+GGG + Chr8:54562593-54562612 None:intergenic 30.0%
!!! CGATTTTTTTTCTTTGCTGT+TGG + Chr8:54562792-54562811 None:intergenic 30.0%
AAAAAGAATAAGGTCTACGG+TGG + Chr8:54562033-54562052 None:intergenic 35.0%
AAACCCAGAATCCGAAAATT+AGG - Chr8:54562614-54562633 MsG0880045123.01.T01:intron 35.0%
AAAGTTGTGGCGTTTATTTC+AGG + Chr8:54564453-54564472 None:intergenic 35.0%
AATTGAGTATCTTGCATGTG+AGG - Chr8:54563925-54563944 MsG0880045123.01.T01:intron 35.0%
ACTTTAACCCCAAATGTCTA+TGG - Chr8:54561986-54562005 MsG0880045123.01.T01:intron 35.0%
ATGATAAACACCAAAGAGCT+CGG + Chr8:54561131-54561150 None:intergenic 35.0%
ATTGAGTATCTTGCATGTGA+GGG - Chr8:54563926-54563945 MsG0880045123.01.T01:intron 35.0%
CAAACCAACAAGAATCATCA+AGG - Chr8:54564627-54564646 MsG0880045123.01.T01:intron 35.0%
CAAAGAATCTGTTGAAGGAA+CGG - Chr8:54564770-54564789 MsG0880045123.01.T01:CDS 35.0%
CGAAGATGATGAAGAAATTG+AGG + Chr8:54562185-54562204 None:intergenic 35.0%
GAATGACAAAGGAAAAGAAG+TGG + Chr8:54561542-54561561 None:intergenic 35.0%
GATGATCGTTGTTATAGATG+CGG - Chr8:54564690-54564709 MsG0880045123.01.T01:intron 35.0%
GTTCCATTGATCCATTAATG+AGG + Chr8:54564148-54564167 None:intergenic 35.0%
TAATGATACTACCTGTCTTG+AGG - Chr8:54564827-54564846 MsG0880045123.01.T01:CDS 35.0%
TGTCGGTCAAATAAATCCAT+GGG - Chr8:54561958-54561977 MsG0880045123.01.T01:intron 35.0%
TGTTATAGATGCGGAAAGAA+AGG - Chr8:54564699-54564718 MsG0880045123.01.T01:intron 35.0%
! AACGCCACAACTTTTAATCA+AGG - Chr8:54564459-54564478 MsG0880045123.01.T01:intron 35.0%
! ACGATCATCATGATTTCTAG+AGG + Chr8:54564679-54564698 None:intergenic 35.0%
! ACGCCACAACTTTTAATCAA+GGG - Chr8:54564460-54564479 MsG0880045123.01.T01:intron 35.0%
! ATTCAAGCATCGTATAAGGA+AGG + Chr8:54561776-54561795 None:intergenic 35.0%
! ATTCAAGCATCTTTAAAGGG+AGG + Chr8:54561690-54561709 None:intergenic 35.0%
! CGATCATCATGATTTCTAGA+GGG + Chr8:54564678-54564697 None:intergenic 35.0%
! CTTGATGATTCTTGTTGGTT+TGG + Chr8:54564629-54564648 None:intergenic 35.0%
! GGTTAGGGTAATAGTCATTT+TGG + Chr8:54561602-54561621 None:intergenic 35.0%
! TATCTTTTACACAGGTACTC+TGG + Chr8:54564750-54564769 None:intergenic 35.0%
! TCTTTGTTGGTGAAGATCTT+TGG - Chr8:54561265-54561284 MsG0880045123.01.T01:CDS 35.0%
! TTCAAGCATCGTATAAGGAA+GGG + Chr8:54561775-54561794 None:intergenic 35.0%
! TTCAAGCATCTTTAAAGGGA+GGG + Chr8:54561689-54561708 None:intergenic 35.0%
! TTTTCTTTGCCAATTCCTCT+GGG + Chr8:54563991-54564010 None:intergenic 35.0%
! TTTTTCTTTGCCAATTCCTC+TGG + Chr8:54563992-54564011 None:intergenic 35.0%
!! AGTCTTATTGCCCTCATTAA+TGG - Chr8:54564134-54564153 MsG0880045123.01.T01:intron 35.0%
!! GTTTGAGTTGATAATGATGG+AGG + Chr8:54562270-54562289 None:intergenic 35.0%
!! TTGGTTTGGTCATATTTGAG+AGG + Chr8:54564615-54564634 None:intergenic 35.0%
!!! TGGAAAGGTTTTGAAGGAAA+TGG + Chr8:54562412-54562431 None:intergenic 35.0%
!!! TTGAAACGATCTTTTGGAGT+TGG + Chr8:54562432-54562451 None:intergenic 35.0%
AACAAGGGTGATTAGATAGC+TGG - Chr8:54561068-54561087 MsG0880045123.01.T01:CDS 40.0%
AACTTAACGCATCCATCTTG+AGG + Chr8:54560987-54561006 None:intergenic 40.0%
ACGATTAATGTGGTGCTCAA+AGG + Chr8:54560943-54560962 None:intergenic 40.0%
ACGCCCTTGATTAAAAGTTG+TGG + Chr8:54564466-54564485 None:intergenic 40.0%
AGGTTTACTTCGTGAGAACA+AGG - Chr8:54561052-54561071 MsG0880045123.01.T01:CDS 40.0%
ATCTGTTGAAGGAACGGAAA+AGG - Chr8:54564776-54564795 MsG0880045123.01.T01:CDS 40.0%
ATTCTTGAGGGAGACGATTT+GGG - Chr8:54563959-54563978 MsG0880045123.01.T01:intron 40.0%
CAAGGTTAGGTGTGACATAT+TGG - Chr8:54561380-54561399 MsG0880045123.01.T01:CDS 40.0%
CTAGCAGTAGTGTAAACTTC+CGG + Chr8:54563823-54563842 None:intergenic 40.0%
CTGGAGACAACTACATAACT+TGG - Chr8:54563891-54563910 MsG0880045123.01.T01:intron 40.0%
CTGTCGGTCAAATAAATCCA+TGG - Chr8:54561957-54561976 MsG0880045123.01.T01:intron 40.0%
GAAAAACAATACGGCAGCAA+TGG + Chr8:54562678-54562697 None:intergenic 40.0%
GCAATATCACTTGAGATGCA+TGG + Chr8:54564302-54564321 None:intergenic 40.0%
GGATATGTCATTGTTCCTGT+AGG + Chr8:54564432-54564451 None:intergenic 40.0%
GGTTTACTTCGTGAGAACAA+GGG - Chr8:54561053-54561072 MsG0880045123.01.T01:CDS 40.0%
GTATGCATACGCTTCTTTGT+TGG - Chr8:54561252-54561271 MsG0880045123.01.T01:CDS 40.0%
TGAGAAGAGGAGGATGATTA+TGG + Chr8:54562311-54562330 None:intergenic 40.0%
TGGATTTATTTGACCGACAG+AGG + Chr8:54561957-54561976 None:intergenic 40.0%
TTAACCCCAAATGTCTATGG+TGG - Chr8:54561989-54562008 MsG0880045123.01.T01:intron 40.0%
TTGGTCCACCATAGACATTT+GGG + Chr8:54561997-54562016 None:intergenic 40.0%
TTTCCAACATGTGCTTAGAG+AGG - Chr8:54561315-54561334 MsG0880045123.01.T01:CDS 40.0%
TTTCCTCTCTAAGCACATGT+TGG + Chr8:54561321-54561340 None:intergenic 40.0%
TTTGGTTACAGCTCTTCTAG+TGG - Chr8:54564362-54564381 MsG0880045123.01.T01:intron 40.0%
! ACAACTTTTAATCAAGGGCG+TGG - Chr8:54564465-54564484 MsG0880045123.01.T01:intron 40.0%
! GAAAGCACGACAACAATTGA+AGG + Chr8:54562745-54562764 None:intergenic 40.0%
! GTCATTGGTCTAAAGCATGT+AGG - Chr8:54564721-54564740 MsG0880045123.01.T01:CDS 40.0%
! TCAAGCATCGTATAAGGAAG+GGG + Chr8:54561774-54561793 None:intergenic 40.0%
! TGCCCTCATTAATGGATCAA+TGG - Chr8:54564142-54564161 MsG0880045123.01.T01:intron 40.0%
!! TGGAGTTGGAAAGGTTTTGA+AGG + Chr8:54562418-54562437 None:intergenic 40.0%
!!! ACGATCTTTTGGAGTTGGAA+AGG + Chr8:54562427-54562446 None:intergenic 40.0%
ACCGACAGAGGTGAAATTGA+GGG + Chr8:54561945-54561964 None:intergenic 45.0%
ATCAGCACGATCGTTTCTAC+CGG - Chr8:54563801-54563820 MsG0880045123.01.T01:intron 45.0%
CGTATGCATACATATGCGAG+CGG + Chr8:54561243-54561262 None:intergenic 45.0%
CTGGTTTCTCTTCTGATGAG+GGG - Chr8:54561087-54561106 MsG0880045123.01.T01:CDS 45.0%
GAAGAGGAGGATGATTATGG+TGG + Chr8:54562308-54562327 None:intergenic 45.0%
GATGCGGAAAGAAAGGTCAT+TGG - Chr8:54564706-54564725 MsG0880045123.01.T01:intron 45.0%
GATTCTTGAGGGAGACGATT+TGG - Chr8:54563958-54563977 MsG0880045123.01.T01:intron 45.0%
GGACTCGACAAGATTCTTGA+GGG - Chr8:54563947-54563966 MsG0880045123.01.T01:intron 45.0%
GGGGTTAAAGTTAACACCCA+TGG + Chr8:54561977-54561996 None:intergenic 45.0%
GGTGGTTATAATCGCCAAAG+AGG - Chr8:54564486-54564505 MsG0880045123.01.T01:intron 45.0%
GTTGGTCCACCATAGACATT+TGG + Chr8:54561998-54562017 None:intergenic 45.0%
TATAATCGCCAAAGAGGACG+TGG - Chr8:54564492-54564511 MsG0880045123.01.T01:intron 45.0%
TCACACCTAACCTTGAAGAG+CGG + Chr8:54561375-54561394 None:intergenic 45.0%
TCCCTCAATTTCACCTCTGT+CGG - Chr8:54561941-54561960 MsG0880045123.01.T01:intron 45.0%
TGGTCCACCATAGACATTTG+GGG + Chr8:54561996-54562015 None:intergenic 45.0%
TGTTGAAGGAACGGAAAAGG+AGG - Chr8:54564779-54564798 MsG0880045123.01.T01:CDS 45.0%
! AGCTGGTTTCTCTTCTGATG+AGG - Chr8:54561085-54561104 MsG0880045123.01.T01:CDS 45.0%
! CATGGTCGAAACCATTTTCG+TGG - Chr8:54564555-54564574 MsG0880045123.01.T01:intron 45.0%
! GCTGGTTTCTCTTCTGATGA+GGG - Chr8:54561086-54561105 MsG0880045123.01.T01:CDS 45.0%
!! ACTTTTAATCAAGGGCGTGG+TGG - Chr8:54564468-54564487 MsG0880045123.01.T01:intron 45.0%
!! CTTGAGGCTGCTGATTTCAT+AGG - Chr8:54564843-54564862 MsG0880045123.01.T01:CDS 45.0%
!! TATTCTCGTTTTGGTGGTCG+TGG - Chr8:54564519-54564538 MsG0880045123.01.T01:intron 45.0%
!! TCTATGGTGGACCAACATTG+TGG - Chr8:54562002-54562021 MsG0880045123.01.T01:intron 45.0%
!!! GCACTTTTTTCCGAGCTCTT+TGG - Chr8:54561118-54561137 MsG0880045123.01.T01:CDS 45.0%
!!! TATTTTAACATAAATAAATA+TGG - Chr8:54561841-54561860 MsG0880045123.01.T01:intron 5.0%
AACGCATCCATCTTGAGGTG+AGG + Chr8:54560982-54561001 None:intergenic 50.0%
AAGTGGACCTCACCTCAAGA+TGG - Chr8:54560972-54560991 MsG0880045123.01.T01:CDS 50.0%
AATAGCCACCACGTCCTCTT+TGG + Chr8:54564503-54564522 None:intergenic 50.0%
AGCGGTCTGGATGTTGAAGA+AGG + Chr8:54561225-54561244 None:intergenic 50.0%
ATTGGCAGACTCCTCCTTCA+GGG - Chr8:54561398-54561417 MsG0880045123.01.T01:CDS 50.0%
GAAATCAGCAGCCTCAAGAC+AGG + Chr8:54564841-54564860 None:intergenic 50.0%
GACCGACAGAGGTGAAATTG+AGG + Chr8:54561946-54561965 None:intergenic 50.0%
GATGGAAGTTGTGGTGCTTC+TGG - Chr8:54561014-54561033 MsG0880045123.01.T01:CDS 50.0%
GGGACTCGACAAGATTCTTG+AGG - Chr8:54563946-54563965 MsG0880045123.01.T01:intron 50.0%
GGTCTGGATGTTGAAGAAGG+CGG + Chr8:54561222-54561241 None:intergenic 50.0%
TATTGGCAGACTCCTCCTTC+AGG - Chr8:54561397-54561416 MsG0880045123.01.T01:CDS 50.0%
TGTGGTGCTTCTGGTGACAT+TGG - Chr8:54561023-54561042 MsG0880045123.01.T01:CDS 50.0%
TTCTCTTCTGATGAGGGGCA+GGG - Chr8:54561092-54561111 MsG0880045123.01.T01:CDS 50.0%
TTGTTCCTGTAGGACGCAAC+TGG + Chr8:54564422-54564441 None:intergenic 50.0%
TTTCTCTTCTGATGAGGGGC+AGG - Chr8:54561091-54561110 MsG0880045123.01.T01:CDS 50.0%
! CGAAACCATTTTCGTGGTGG+AGG - Chr8:54564561-54564580 MsG0880045123.01.T01:intron 50.0%
! CGTGGTGGCTATTCTCGTTT+TGG - Chr8:54564510-54564529 MsG0880045123.01.T01:intron 50.0%
! GGTCGAAACCATTTTCGTGG+TGG - Chr8:54564558-54564577 MsG0880045123.01.T01:intron 50.0%
! GGTGGCTATTCTCGTTTTGG+TGG - Chr8:54564513-54564532 MsG0880045123.01.T01:intron 50.0%
!! AACGCTGACTTCAACCCTGA+AGG + Chr8:54561415-54561434 None:intergenic 50.0%
!! CGGTGGACTATCCACAATGT+TGG + Chr8:54562016-54562035 None:intergenic 50.0%
!! TCTGGTGACATTGGCTCTAG+AGG - Chr8:54561032-54561051 MsG0880045123.01.T01:CDS 50.0%
AACCACCAGTTGCGTCCTAC+AGG - Chr8:54564414-54564433 MsG0880045123.01.T01:intron 55.0%
AATCGCCAAAGAGGACGTGG+TGG - Chr8:54564495-54564514 MsG0880045123.01.T01:intron 55.0%
CAGGGTTGAAGTCAGCGTTG+AGG - Chr8:54561416-54561435 MsG0880045123.01.T01:CDS 55.0%
CTCGACCGCTCTTCAAGGTT+AGG - Chr8:54561367-54561386 MsG0880045123.01.T01:CDS 55.0%
GCATACATATGCGAGCGGTC+TGG + Chr8:54561238-54561257 None:intergenic 55.0%
GGGAGATGTCCCAGAGGAAT+TGG - Chr8:54563979-54563998 MsG0880045123.01.T01:intron 55.0%
TCTCTTCTGATGAGGGGCAG+GGG - Chr8:54561093-54561112 MsG0880045123.01.T01:CDS 55.0%
TTCCTGTAGGACGCAACTGG+TGG + Chr8:54564419-54564438 None:intergenic 55.0%
! CGATTTGGGAGATGTCCCAG+AGG - Chr8:54563973-54563992 MsG0880045123.01.T01:intron 55.0%
! GCTGACTTCAACCCTGAAGG+AGG + Chr8:54561412-54561431 None:intergenic 55.0%
CACGGCCTCCACCACGAAAA+TGG + Chr8:54564569-54564588 None:intergenic 60.0%
CCAAGCTCGACCGCTCTTCA+AGG - Chr8:54561362-54561381 MsG0880045123.01.T01:CDS 60.0%
CCTTGAAGAGCGGTCGAGCT+TGG + Chr8:54561365-54561384 None:intergenic 60.0%
CGTGGTCGAGGCAGAAGTCA+TGG - Chr8:54564537-54564556 MsG0880045123.01.T01:intron 60.0%
!! CATTTTCGTGGTGGAGGCCG+TGG - Chr8:54564567-54564586 MsG0880045123.01.T01:intron 60.0%
!! CGTTTTGGTGGTCGTGGTCG+AGG - Chr8:54564525-54564544 MsG0880045123.01.T01:intron 60.0%
! CGTGGTGGAGGCCGTGGAAG+AGG - Chr8:54564573-54564592 MsG0880045123.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 54560906 54564887 54560906 ID=MsG0880045123.01;Name=MsG0880045123.01
Chr8 mRNA 54560906 54564887 54560906 ID=MsG0880045123.01.T01;Parent=MsG0880045123.01;Name=MsG0880045123.01.T01;_AED=0.50;_eAED=0.74;_QI=0|0|0|1|1|1|2|0|330
Chr8 exon 54564719 54564887 54564719 ID=MsG0880045123.01.T01:exon:23557;Parent=MsG0880045123.01.T01
Chr8 exon 54560906 54561729 54560906 ID=MsG0880045123.01.T01:exon:23556;Parent=MsG0880045123.01.T01
Chr8 CDS 54564719 54564887 54564719 ID=MsG0880045123.01.T01:cds;Parent=MsG0880045123.01.T01
Chr8 CDS 54560906 54561729 54560906 ID=MsG0880045123.01.T01:cds;Parent=MsG0880045123.01.T01
Gene Sequence

>MsG0880045123.01.T01

ATGCATACTGGCCTAATTTCTGTTAACATTCGCTCCTTTGAGCACCACATTAATCGTAACATTGTTAAGTGGACCTCACCTCAAGATGGATGCGTTAAGTTGAATATTGATGGAAGTTGTGGTGCTTCTGGTGACATTGGCTCTAGAGGTTTACTTCGTGAGAACAAGGAGTATTCGAATGCAAAAGATCCCAAGATTTTATGGGACAAAATTAAGGCAAGATTTGGGCATCAGAAGAAAGTCTTATTGCCCTCATTAATGGATCAATGGAACAAATTAAGGTTCCAAGATTATAAAACTGTTGTTGCATATAACTCTGCTATGCATCAAATTATTGCACAATTAGAATTTTGTGGCAAAACTATAACTGAAAAGGAAAAGTTGGAGAAAACATTCTCAACTTTCCATGCATCTCAAGTGATATTGCAACAACAATATAGAATGAGGGAATTTACTGAATACTCTGATTTGGTTACAGCTCTTCTAGTGGCTGAACAAAATAATGAGCTGCTCATTAAAAACCACCAGTTGCGTCCTACAGGAACAATGACATATCCTGAAATAAACGCCACAACTTTTAATCAAGGGCGTGGTGGTTATAATCGCCAAAGAGGACGTGGTGGCTATTCTCGTTTTGGTGGTCGTGGTCGAGGCAGAAGTCATGGTCGAAACCATTTTCGTGGTGGAGGCCGTGGAAGAGGTTATATGAATTATAGGCCTCTCAAATATGACCAAACCAACAAGAATCATCAAGGTAAAGGTAAAAACATACAGGAAGGTCCCTCTAGAAATCATGATGATCGTTGTTATAGATGCGGAAAGAAAGGTCATTGGTCTAAAGCATGTAGGACACCAGAGTACCTGTGTAAAAGATACAAAGAATCTGTTGAAGGAACGGAAAAGGAGGTTAATTTTAATGAAATTGAACCCAATAATGATACTACCTGTCTTGAGGCTGCTGATTTCATAGGAAAAGATATTAATATGGATTAA
Protein sequence

>MsG0880045123.01.T01

MHTGLISVNIRSFEHHINRNIVKWTSPQDGCVKLNIDGSCGASGDIGSRGLLRENKEYSNAKDPKILWDKIKARFGHQKKVLLPSLMDQWNKLRFQDYKTVVAYNSAMHQIIAQLEFCGKTITEKEKLEKTFSTFHASQVILQQQYRMREFTEYSDLVTALLVAEQNNELLIKNHQLRPTGTMTYPEINATTFNQGRGGYNRQRGRGGYSRFGGRGRGRSHGRNHFRGGGRGRGYMNYRPLKYDQTNKNHQGKGKNIQEGPSRNHDDRCYRCGKKGHWSKACRTPEYLCKRYKESVEGTEKEVNFNEIEPNNDTTCLEAADFIGKDINMD*