Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045171.01.T01 | XP_003628793.2 | 90.11 | 182 | 18 | 0 | 1 | 182 | 1 | 182 | 1.35E-110 | 327 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045171.01.T01 | Q9FIQ5 | 63.187 | 182 | 67 | 0 | 1 | 182 | 1 | 182 | 7.44E-70 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045171.01.T01 | G7LH20 | 90.110 | 182 | 18 | 0 | 1 | 182 | 1 | 182 | 6.43e-111 | 327 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015736.01 | MsG0880045171.01 | 0.800662 | 1.297295e-48 | 6.075127e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045171.01.T01 | MTR_8g066790 | 90.110 | 182 | 18 | 0 | 1 | 182 | 1 | 182 | 1.63e-114 | 327 |
| MsG0880045171.01.T01 | MTR_5g030070 | 61.000 | 200 | 78 | 0 | 1 | 200 | 1 | 200 | 1.25e-71 | 218 |
| MsG0880045171.01.T01 | MTR_8g101600 | 40.609 | 197 | 117 | 0 | 1 | 197 | 1 | 197 | 5.46e-46 | 153 |
| MsG0880045171.01.T01 | MTR_4g129670 | 41.989 | 181 | 103 | 1 | 1 | 179 | 1 | 181 | 1.34e-43 | 147 |
| MsG0880045171.01.T01 | MTR_4g061010 | 43.860 | 171 | 91 | 2 | 1 | 166 | 1 | 171 | 2.35e-43 | 146 |
| MsG0880045171.01.T01 | MTR_6g033335 | 37.363 | 182 | 111 | 2 | 3 | 184 | 4 | 182 | 7.93e-38 | 132 |
| MsG0880045171.01.T01 | MTR_1g095530 | 35.135 | 185 | 117 | 2 | 3 | 187 | 4 | 185 | 2.10e-31 | 115 |
| MsG0880045171.01.T01 | MTR_8g097280 | 33.333 | 168 | 111 | 1 | 1 | 168 | 3 | 169 | 1.24e-27 | 106 |
| MsG0880045171.01.T01 | MTR_4g112570 | 32.402 | 179 | 120 | 1 | 3 | 180 | 6 | 184 | 1.40e-24 | 97.4 |
| MsG0880045171.01.T01 | MTR_2g056130 | 29.508 | 122 | 86 | 0 | 25 | 146 | 25 | 146 | 1.17e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045171.01.T01 | AT5G46700 | 63.187 | 182 | 67 | 0 | 1 | 182 | 1 | 182 | 7.58e-71 | 216 |
| MsG0880045171.01.T01 | AT3G45600 | 38.743 | 191 | 112 | 2 | 1 | 186 | 1 | 191 | 9.88e-44 | 145 |
| MsG0880045171.01.T01 | AT4G28050 | 39.779 | 181 | 107 | 1 | 4 | 182 | 5 | 185 | 9.22e-43 | 144 |
| MsG0880045171.01.T01 | AT3G45600 | 39.037 | 187 | 109 | 2 | 1 | 182 | 1 | 187 | 2.45e-42 | 144 |
| MsG0880045171.01.T01 | AT2G23810 | 37.017 | 181 | 112 | 1 | 4 | 182 | 5 | 185 | 8.82e-40 | 137 |
| MsG0880045171.01.T01 | AT4G30430 | 35.165 | 182 | 116 | 1 | 3 | 182 | 4 | 185 | 2.92e-36 | 128 |
| MsG0880045171.01.T01 | AT2G19580 | 41.304 | 184 | 105 | 3 | 1 | 182 | 1 | 183 | 4.34e-34 | 122 |
| MsG0880045171.01.T01 | AT3G12090 | 36.158 | 177 | 110 | 2 | 3 | 179 | 4 | 177 | 3.03e-32 | 118 |
| MsG0880045171.01.T01 | AT5G60220 | 37.433 | 187 | 112 | 2 | 1 | 182 | 1 | 187 | 3.37e-32 | 119 |
| MsG0880045171.01.T01 | AT1G63260 | 29.891 | 184 | 124 | 2 | 1 | 180 | 3 | 185 | 1.06e-27 | 104 |
| MsG0880045171.01.T01 | AT1G63260 | 30.539 | 167 | 115 | 1 | 1 | 167 | 3 | 168 | 2.69e-27 | 103 |
| MsG0880045171.01.T01 | AT1G63260 | 30.539 | 167 | 115 | 1 | 1 | 167 | 3 | 168 | 7.91e-27 | 103 |
| MsG0880045171.01.T01 | AT1G63260 | 30.539 | 167 | 115 | 1 | 1 | 167 | 3 | 168 | 8.65e-27 | 103 |
| MsG0880045171.01.T01 | AT1G63260 | 30.539 | 167 | 115 | 1 | 1 | 167 | 3 | 168 | 8.65e-27 | 103 |
| MsG0880045171.01.T01 | AT1G63260 | 30.539 | 167 | 115 | 1 | 1 | 167 | 3 | 168 | 9.61e-27 | 103 |
| MsG0880045171.01.T01 | AT1G18520 | 31.492 | 181 | 120 | 3 | 3 | 180 | 4 | 183 | 2.22e-24 | 97.1 |
| MsG0880045171.01.T01 | AT4G23410 | 31.351 | 185 | 124 | 2 | 3 | 187 | 4 | 185 | 1.75e-19 | 84.3 |
| MsG0880045171.01.T01 | AT2G03840 | 24.183 | 153 | 110 | 2 | 19 | 166 | 32 | 183 | 3.11e-13 | 67.0 |
Find 34 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATCATTGATTAAGTAAT+TGG | 0.136459 | 8:-55124059 | None:intergenic |
| CAATAATTGGTGCTGGAATT+TGG | 0.184709 | 8:+55123564 | MsG0880045171.01.T01:CDS |
| CAAATTCCAGCACCAATTAT+TGG | 0.222813 | 8:-55123563 | None:intergenic |
| GGGTTAGAAGCTCACATAAA+TGG | 0.224537 | 8:+55123873 | MsG0880045171.01.T01:CDS |
| TATTCTAAATATGACCTATT+AGG | 0.288046 | 8:-55123815 | None:intergenic |
| CTCCTCTCAATCCCAATAAT+TGG | 0.310666 | 8:+55123551 | MsG0880045171.01.T01:CDS |
| TTGGTTTCATTGGAGCCTTT+TGG | 0.313785 | 8:+55123675 | MsG0880045171.01.T01:CDS |
| AAATTCCAGCACCAATTATT+GGG | 0.333661 | 8:-55123562 | None:intergenic |
| GTGTATTCGGTTACACTTAG+AGG | 0.346299 | 8:+55123776 | MsG0880045171.01.T01:CDS |
| TGCTCACCTATCACCCTTGC+AGG | 0.349924 | 8:+55123979 | MsG0880045171.01.T01:CDS |
| GTTGGTATGGTTGGTTTCAT+TGG | 0.355504 | 8:+55123665 | MsG0880045171.01.T01:CDS |
| CCAATTACTTAATCAATGAT+TGG | 0.385715 | 8:+55124059 | MsG0880045171.01.T01:CDS |
| ATGGGAATATTGAACCTAAT+AGG | 0.401642 | 8:+55123801 | MsG0880045171.01.T01:CDS |
| GATTTACCTGCAAGGGTGAT+AGG | 0.408328 | 8:-55123985 | None:intergenic |
| AAATGATCACTGGCCATTGT+AGG | 0.408428 | 8:-55123622 | None:intergenic |
| TAAAGAAATCTTGAGCAATT+CGG | 0.414307 | 8:-55123955 | None:intergenic |
| GGTTAGAAGCTCACATAAAT+GGG | 0.454130 | 8:+55123874 | MsG0880045171.01.T01:CDS |
| TCAATCCCAATAATTGGTGC+TGG | 0.495286 | 8:+55123557 | MsG0880045171.01.T01:CDS |
| AGGTAGAATATGAGAAGCAT+TGG | 0.498572 | 8:-55123704 | None:intergenic |
| GCATTGAGCAACAATGTGAT+AGG | 0.498985 | 8:+55123506 | MsG0880045171.01.T01:CDS |
| AAGCATTGGGATTCTCCAAA+AGG | 0.516728 | 8:-55123690 | None:intergenic |
| CAAATCAAGTAATGGACTAG+TGG | 0.540302 | 8:-55124007 | None:intergenic |
| GGTAGAATATGAGAAGCATT+GGG | 0.553523 | 8:-55123703 | None:intergenic |
| AGTATCCCTAAAATGATCAC+TGG | 0.555016 | 8:-55123632 | None:intergenic |
| CACCAATTATTGGGATTGAG+AGG | 0.558225 | 8:-55123553 | None:intergenic |
| TCGGTTACACTTAGAGGACA+TGG | 0.562986 | 8:+55123782 | MsG0880045171.01.T01:CDS |
| AAAACGTACAAATCAAGTAA+TGG | 0.572694 | 8:-55124015 | None:intergenic |
| AGAAGCTCACATAAATGGGA+TGG | 0.594740 | 8:+55123878 | MsG0880045171.01.T01:CDS |
| ACTAGTGGATTTACCTGCAA+GGG | 0.618595 | 8:-55123992 | None:intergenic |
| GACTAGTGGATTTACCTGCA+AGG | 0.628765 | 8:-55123993 | None:intergenic |
| GTCATATTTAGAATATCGTG+TGG | 0.633248 | 8:+55123823 | MsG0880045171.01.T01:CDS |
| CGGTTACACTTAGAGGACAT+GGG | 0.635110 | 8:+55123783 | MsG0880045171.01.T01:CDS |
| CATGTGTCAAAATCCTACAA+TGG | 0.641159 | 8:+55123609 | MsG0880045171.01.T01:CDS |
| TGAGCATGAACATAGCAATG+AGG | 0.718294 | 8:-55123724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTCTAAATATGACCTATT+AGG | - | Chr8:55123818-55123837 | None:intergenic | 20.0% |
| !!! | ACTAATTTTAATTGTTGGTA+TGG | + | Chr8:55123652-55123671 | MsG0880045171.01.T01:CDS | 20.0% |
| ! | AAAACGTACAAATCAAGTAA+TGG | - | Chr8:55124018-55124037 | None:intergenic | 25.0% |
| ! | CCAATTACTTAATCAATGAT+TGG | + | Chr8:55124059-55124078 | MsG0880045171.01.T01:CDS | 25.0% |
| ! | TAAAGAAATCTTGAGCAATT+CGG | - | Chr8:55123958-55123977 | None:intergenic | 25.0% |
| !! | CCAATCATTGATTAAGTAAT+TGG | - | Chr8:55124062-55124081 | None:intergenic | 25.0% |
| !!! | ATTTTAATTGTTGGTATGGT+TGG | + | Chr8:55123656-55123675 | MsG0880045171.01.T01:CDS | 25.0% |
| !!! | GGGATACTAATTTTAATTGT+TGG | + | Chr8:55123647-55123666 | MsG0880045171.01.T01:CDS | 25.0% |
| !!! | TTTGGTGATTTTTGTGTATT+CGG | + | Chr8:55123763-55123782 | MsG0880045171.01.T01:CDS | 25.0% |
| AAATTCCAGCACCAATTATT+GGG | - | Chr8:55123565-55123584 | None:intergenic | 30.0% | |
| ATGGGAATATTGAACCTAAT+AGG | + | Chr8:55123801-55123820 | MsG0880045171.01.T01:CDS | 30.0% | |
| ! | GTCATATTTAGAATATCGTG+TGG | + | Chr8:55123823-55123842 | MsG0880045171.01.T01:CDS | 30.0% |
| ! | GTGTGGATGATTTTTCATTT+TGG | + | Chr8:55123840-55123859 | MsG0880045171.01.T01:CDS | 30.0% |
| !! | TTCATGCTCATTGTTTTGTT+GGG | + | Chr8:55123737-55123756 | MsG0880045171.01.T01:CDS | 30.0% |
| AGTATCCCTAAAATGATCAC+TGG | - | Chr8:55123635-55123654 | None:intergenic | 35.0% | |
| CAAATTCCAGCACCAATTAT+TGG | - | Chr8:55123566-55123585 | None:intergenic | 35.0% | |
| CATGTGTCAAAATCCTACAA+TGG | + | Chr8:55123609-55123628 | MsG0880045171.01.T01:CDS | 35.0% | |
| GGTTAGAAGCTCACATAAAT+GGG | + | Chr8:55123874-55123893 | MsG0880045171.01.T01:CDS | 35.0% | |
| ! | AATGGCCAGTGATCATTTTA+GGG | + | Chr8:55123627-55123646 | MsG0880045171.01.T01:CDS | 35.0% |
| ! | AGGTAGAATATGAGAAGCAT+TGG | - | Chr8:55123707-55123726 | None:intergenic | 35.0% |
| !! | CAAATCAAGTAATGGACTAG+TGG | - | Chr8:55124010-55124029 | None:intergenic | 35.0% |
| !! | CAATAATTGGTGCTGGAATT+TGG | + | Chr8:55123564-55123583 | MsG0880045171.01.T01:CDS | 35.0% |
| !! | CATTGTTTTGTTGGGTTCTT+TGG | + | Chr8:55123745-55123764 | MsG0880045171.01.T01:CDS | 35.0% |
| !! | GGTAGAATATGAGAAGCATT+GGG | - | Chr8:55123706-55123725 | None:intergenic | 35.0% |
| !! | GTTCATGCTCATTGTTTTGT+TGG | + | Chr8:55123736-55123755 | MsG0880045171.01.T01:CDS | 35.0% |
| !!! | TTCATTTTGGCTACGTAGAA+GGG | + | Chr8:55123853-55123872 | MsG0880045171.01.T01:CDS | 35.0% |
| !!! | TTTCATTTTGGCTACGTAGA+AGG | + | Chr8:55123852-55123871 | MsG0880045171.01.T01:CDS | 35.0% |
| AAATGATCACTGGCCATTGT+AGG | - | Chr8:55123625-55123644 | None:intergenic | 40.0% | |
| AGAAGCTCACATAAATGGGA+TGG | + | Chr8:55123878-55123897 | MsG0880045171.01.T01:CDS | 40.0% | |
| CACCAATTATTGGGATTGAG+AGG | - | Chr8:55123556-55123575 | None:intergenic | 40.0% | |
| CTCCTCTCAATCCCAATAAT+TGG | + | Chr8:55123551-55123570 | MsG0880045171.01.T01:CDS | 40.0% | |
| GCATTGAGCAACAATGTGAT+AGG | + | Chr8:55123506-55123525 | MsG0880045171.01.T01:CDS | 40.0% | |
| GGGTTAGAAGCTCACATAAA+TGG | + | Chr8:55123873-55123892 | MsG0880045171.01.T01:CDS | 40.0% | |
| GTGTATTCGGTTACACTTAG+AGG | + | Chr8:55123776-55123795 | MsG0880045171.01.T01:CDS | 40.0% | |
| TCAATCCCAATAATTGGTGC+TGG | + | Chr8:55123557-55123576 | MsG0880045171.01.T01:CDS | 40.0% | |
| TGAGCATGAACATAGCAATG+AGG | - | Chr8:55123727-55123746 | None:intergenic | 40.0% | |
| ! | CAATGGCCAGTGATCATTTT+AGG | + | Chr8:55123626-55123645 | MsG0880045171.01.T01:CDS | 40.0% |
| ! | TTGGTTTCATTGGAGCCTTT+TGG | + | Chr8:55123675-55123694 | MsG0880045171.01.T01:CDS | 40.0% |
| !! | AAGCATTGGGATTCTCCAAA+AGG | - | Chr8:55123693-55123712 | None:intergenic | 40.0% |
| !! | ACTAGTGGATTTACCTGCAA+GGG | - | Chr8:55123995-55124014 | None:intergenic | 40.0% |
| !! | GTTGGTATGGTTGGTTTCAT+TGG | + | Chr8:55123665-55123684 | MsG0880045171.01.T01:CDS | 40.0% |
| CGGTTACACTTAGAGGACAT+GGG | + | Chr8:55123783-55123802 | MsG0880045171.01.T01:CDS | 45.0% | |
| GATTTACCTGCAAGGGTGAT+AGG | - | Chr8:55123988-55124007 | None:intergenic | 45.0% | |
| TCGGTTACACTTAGAGGACA+TGG | + | Chr8:55123782-55123801 | MsG0880045171.01.T01:CDS | 45.0% | |
| !! | GACTAGTGGATTTACCTGCA+AGG | - | Chr8:55123996-55124015 | None:intergenic | 45.0% |
| TGCTCACCTATCACCCTTGC+AGG | + | Chr8:55123979-55123998 | MsG0880045171.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 55123503 | 55124108 | 55123503 | ID=MsG0880045171.01;Name=MsG0880045171.01 |
| Chr8 | mRNA | 55123503 | 55124108 | 55123503 | ID=MsG0880045171.01.T01;Parent=MsG0880045171.01;Name=MsG0880045171.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|201 |
| Chr8 | exon | 55123503 | 55124108 | 55123503 | ID=MsG0880045171.01.T01:exon:23805;Parent=MsG0880045171.01.T01 |
| Chr8 | CDS | 55123503 | 55124108 | 55123503 | ID=MsG0880045171.01.T01:cds;Parent=MsG0880045171.01.T01 |
| Gene Sequence |
| Protein sequence |
