AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015736.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015736.01.T01 MTR_3g075450 54.545 264 60 7 155 358 378 641 4.95e-74 243
MsG0380015736.01.T01 MTR_3g075450 96.154 104 4 0 1 104 1 104 2.36e-58 201
MsG0380015736.01.T01 MTR_3g075450 100.000 41 0 0 106 146 188 228 5.49e-18 85.9
MsG0380015736.01.T01 MTR_8g089660 33.208 265 115 7 155 358 376 639 2.03e-31 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015736.01.T01 AT3G01680 26.493 268 119 8 153 348 388 649 6.15e-16 80.1

Find 80 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGATTTCATCAACAATT+TGG 0.168351 3:+74439436 None:intergenic
TGGCATTGATCCTGCACTTT+TGG 0.203402 3:-74436941 MsG0380015736.01.T01:CDS
CCATTCCTATTGCTACCTAT+TGG 0.227612 3:-74438128 MsG0380015736.01.T01:CDS
CAAATGCTCTTCACATGATA+TGG 0.269995 3:-74437052 MsG0380015736.01.T01:CDS
AGTTTGAGAGATTTGATAAA+TGG 0.282401 3:-74436385 MsG0380015736.01.T01:CDS
CATATCATGTGAAGAGCATT+TGG 0.305613 3:+74437053 None:intergenic
CATTCCTATTGCTACCTATT+GGG 0.316845 3:-74438127 MsG0380015736.01.T01:intron
CATGTTGCTTTGTCCACAAC+TGG 0.342091 3:+74437245 None:intergenic
CATGTGCATTGTTGAATTCA+AGG 0.348701 3:-74438207 MsG0380015736.01.T01:CDS
AGCCTTGAGTTGCTTGTTGA+TGG 0.355975 3:-74436961 MsG0380015736.01.T01:CDS
CTCTTCACATGATATGGATT+GGG 0.365746 3:-74437046 MsG0380015736.01.T01:CDS
TGTTAGAAGCAGCTGATAAC+TGG 0.369862 3:+74439577 None:intergenic
CCTTGTGGAAGCAAGAGATT+TGG 0.382186 3:-74436984 MsG0380015736.01.T01:CDS
TCATCAACAATTTGGATAAG+TGG 0.405497 3:+74439444 None:intergenic
ACATCGATTTCACGACCATC+AGG 0.425726 3:+74439471 None:intergenic
TTCTTCTTCTCCACTTGTGA+TGG 0.431789 3:+74437008 None:intergenic
ATGACCCAATAGGTAGCAAT+AGG 0.437496 3:+74438123 None:intergenic
CTGTAGAGGACCATCTGAAA+TGG 0.452637 3:-74436437 MsG0380015736.01.T01:CDS
GCTCTTCACATGATATGGAT+TGG 0.465516 3:-74437047 MsG0380015736.01.T01:CDS
TAGGATTGAACAAGATGGTT+GGG 0.467822 3:-74436357 MsG0380015736.01.T01:CDS
CACTAGCCATGTTACTGAAA+AGG 0.478680 3:+74439610 None:intergenic
TTAATATAATCATTCAATGC+TGG 0.481704 3:+74436332 None:intergenic
TCTGAAATGGCTAGGGCTAA+AGG 0.482330 3:-74436424 MsG0380015736.01.T01:CDS
CGACGGTAGTGACCGAGGTT+GGG 0.484160 3:-74436467 MsG0380015736.01.T01:CDS
AATACTTCTGATGACCCAAT+AGG 0.487922 3:+74438113 None:intergenic
ATTCGATATGCTTGATGCGC+TGG 0.489847 3:-74439016 MsG0380015736.01.T01:CDS
ATCATTGCTCGAAGTGTTGA+GGG 0.490421 3:-74439408 MsG0380015736.01.T01:CDS
AGCCTTCACAAGCTTGTTGA+GGG 0.497030 3:+74438244 None:intergenic
ATATCATGTGAAGAGCATTT+GGG 0.503373 3:+74437054 None:intergenic
TCCTTGTTGTAGATGACAGA+AGG 0.509373 3:-74436799 MsG0380015736.01.T01:CDS
AGACACACTATGAGATGGTA+TGG 0.509954 3:-74437271 MsG0380015736.01.T01:CDS
TAACAGAATGCAGCTAAAGA+AGG 0.515235 3:-74439559 MsG0380015736.01.T01:CDS
GAGGACCATCTGAAATGGCT+AGG 0.526776 3:-74436432 MsG0380015736.01.T01:CDS
AATCATTGCTCGAAGTGTTG+AGG 0.530438 3:-74439409 MsG0380015736.01.T01:CDS
CAGTCCACTCATGCCCCTGA+TGG 0.532299 3:-74439486 MsG0380015736.01.T01:CDS
CAGCCTTCACAAGCTTGTTG+AGG 0.540942 3:+74438243 None:intergenic
TCGACGGTAGTGACCGAGGT+TGG 0.542386 3:-74436468 MsG0380015736.01.T01:CDS
GGAGCACTTGCAACAGACAT+AGG 0.550601 3:+74438149 None:intergenic
GGACTGAACTTGTTTCATCA+TGG 0.553456 3:+74439503 None:intergenic
GATTTGATAAATGGAAGCAT+AGG 0.553521 3:-74436376 MsG0380015736.01.T01:CDS
TTTGGCATTTCCATCACAAG+TGG 0.555216 3:-74437018 MsG0380015736.01.T01:CDS
TAAACATGAGACAGATACAT+TGG 0.555533 3:-74439061 MsG0380015736.01.T01:CDS
TGGGAGGACAATCTTCCTTG+TGG 0.557269 3:+74437073 None:intergenic
CCAAATCTCTTGCTTCCACA+AGG 0.558786 3:+74436984 None:intergenic
ATAGGATTGAACAAGATGGT+TGG 0.565947 3:-74436358 MsG0380015736.01.T01:CDS
TTAGCCCTAGCCATTTCAGA+TGG 0.573010 3:+74436427 None:intergenic
ATACAAAGATGCTAGATCAA+GGG 0.575211 3:-74437297 MsG0380015736.01.T01:intron
ACGACCATCAGGGGCATGAG+TGG 0.576810 3:+74439482 None:intergenic
TGCCATCAACAAGCAACTCA+AGG 0.577841 3:+74436959 None:intergenic
CCAATAGGTAGCAATAGGAA+TGG 0.578587 3:+74438128 None:intergenic
TACAAAGATGCTAGATCAAG+GGG 0.585947 3:-74437296 MsG0380015736.01.T01:intron
TCTTCACATGATATGGATTG+GGG 0.587964 3:-74437045 MsG0380015736.01.T01:CDS
CATCGATTTCACGACCATCA+GGG 0.587965 3:+74439472 None:intergenic
CATACCCAGTCCAAAAGTGC+AGG 0.589037 3:+74436931 None:intergenic
AGCTTCGACGGTAGTGACCG+AGG 0.593982 3:-74436472 MsG0380015736.01.T01:CDS
ATGACTGTGCTTAGCTTCGA+CGG 0.596323 3:-74436484 MsG0380015736.01.T01:CDS
AGGACCATCTGAAATGGCTA+GGG 0.600106 3:-74436431 MsG0380015736.01.T01:CDS
CAGTTGTGGACAAAGCAACA+TGG 0.602977 3:-74437244 MsG0380015736.01.T01:CDS
CTTGAATTCAACAATGCACA+TGG 0.609877 3:+74438208 None:intergenic
TACAGAATATTGCCCAACCT+CGG 0.610840 3:+74436455 None:intergenic
ACTCAAAACTACAACATGGT+AGG 0.611324 3:-74436540 MsG0380015736.01.T01:CDS
CTAGTAGCTCTTGATCCACA+AGG 0.616181 3:-74437088 MsG0380015736.01.T01:intron
GATGGTATGGATTCCAGTTG+TGG 0.618882 3:-74437258 MsG0380015736.01.T01:CDS
TGCCCTCAACAAGCTTGTGA+AGG 0.619838 3:-74438246 MsG0380015736.01.T01:CDS
ATCGATTTCACGACCATCAG+GGG 0.621216 3:+74439473 None:intergenic
TCTTCTGAAATATATTCAGA+TGG 0.623277 3:+74438179 None:intergenic
TCCTTCTGTCATCTACAACA+AGG 0.626759 3:+74436798 None:intergenic
ATGGAGGCGAAGATCTAGAG+TGG 0.631690 3:-74436759 MsG0380015736.01.T01:CDS
GGGTCACGAACACTGTGCCA+CGG 0.632031 3:+74437176 None:intergenic
GGTTGGGCAATATTCTGTAG+AGG 0.638960 3:-74436451 MsG0380015736.01.T01:CDS
AAAATCATATGTCTATATGG+AGG 0.645145 3:-74436775 MsG0380015736.01.T01:CDS
GGGTGAGACACACTATGAGA+TGG 0.645383 3:-74437276 MsG0380015736.01.T01:CDS
GTGGAGAAGAAGAATCCTTG+TGG 0.645436 3:-74436999 MsG0380015736.01.T01:CDS
AAGCATAGGATTGAACAAGA+TGG 0.647135 3:-74436362 MsG0380015736.01.T01:CDS
ATCTTCAATCAACAATGCCG+TGG 0.650780 3:-74437193 MsG0380015736.01.T01:CDS
GAAGCAGCTGATAACTGGCG+AGG 0.655882 3:+74439582 None:intergenic
GCACTTGCAACAGACATAGG+AGG 0.664813 3:+74438152 None:intergenic
TCATGTGAAGAGCATTTGGG+AGG 0.688950 3:+74437057 None:intergenic
CTTCACATGATATGGATTGG+GGG 0.690656 3:-74437044 MsG0380015736.01.T01:CDS
TTGTACTCAAAACTACAACA+TGG 0.705926 3:-74436544 MsG0380015736.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTACTATTAATATGATTA+TGG - Chr3:74436883-74436902 MsG0380015736.01.T01:intron 15.0%
!!! ACATAATTTGAAACTTAATT+TGG - Chr3:74438195-74438214 MsG0380015736.01.T01:CDS 15.0%
!! AATATTTATTACTGTTGTCT+AGG - Chr3:74437078-74437097 MsG0380015736.01.T01:CDS 20.0%
!! ATAAACTGGTTAAAAACAAA+TGG + Chr3:74436849-74436868 None:intergenic 20.0%
!! CAAAAACTATCTAAATAAAC+TGG + Chr3:74436863-74436882 None:intergenic 20.0%
!! CTAAAATGAAAATGATCAAT+TGG - Chr3:74437462-74437481 MsG0380015736.01.T01:intron 20.0%
!! GATCAATTGGAAAAATATAT+TGG - Chr3:74437475-74437494 MsG0380015736.01.T01:intron 20.0%
!!! CAATTGATCATTTTCATTTT+AGG + Chr3:74437464-74437483 None:intergenic 20.0%
!!! TATGACACATTTTAGTAATA+TGG + Chr3:74437409-74437428 None:intergenic 20.0%
!!! TGTCATTTTTGTTGAAATTT+CGG - Chr3:74437429-74437448 MsG0380015736.01.T01:intron 20.0%
!!! TTTAGCAATACAAAACTTTT+TGG - Chr3:74438267-74438286 MsG0380015736.01.T01:intron 20.0%
! AAAATCATATGTCTATATGG+AGG - Chr3:74439215-74439234 MsG0380015736.01.T01:intron 25.0%
! AACACAATATCTCATAAAAG+TGG + Chr3:74438249-74438268 None:intergenic 25.0%
! AAGTATGTTAATGTCGAAAA+AGG - Chr3:74437144-74437163 MsG0380015736.01.T01:intron 25.0%
! AATAAAGTCTCACAAACAAA+GGG + Chr3:74438529-74438548 None:intergenic 25.0%
! AGTTTGAGAGATTTGATAAA+TGG - Chr3:74439605-74439624 MsG0380015736.01.T01:exon 25.0%
! ATCAAAAGTTAAGTTAAGAG+AGG + Chr3:74437189-74437208 None:intergenic 25.0%
! ATGAACTTTCATGAAAGTTA+AGG + Chr3:74437050-74437069 None:intergenic 25.0%
! GGAAAAATCATATGTCTATA+TGG - Chr3:74439212-74439231 MsG0380015736.01.T01:intron 25.0%
! TAATAAAGTCTCACAAACAA+AGG + Chr3:74438530-74438549 None:intergenic 25.0%
! TAGATGGAACTACAAAATTA+AGG - Chr3:74438470-74438489 MsG0380015736.01.T01:intron 25.0%
! TCAAGATTTCATCAACAATT+TGG + Chr3:74436557-74436576 None:intergenic 25.0%
! TTAAGTATATCAAAGAAGTG+TGG - Chr3:74438859-74438878 MsG0380015736.01.T01:intron 25.0%
! TTGATATACTTAATCACTGA+TGG + Chr3:74438852-74438871 None:intergenic 25.0%
!! AACTTAATTTGGATAGTCTA+CGG - Chr3:74438206-74438225 MsG0380015736.01.T01:CDS 25.0%
!! AAGTTGACCAATGATTATTT+TGG - Chr3:74436756-74436775 MsG0380015736.01.T01:CDS 25.0%
!!! AAGATTTTAATTAAACCACG+AGG + Chr3:74438563-74438582 None:intergenic 25.0%
!!! ATACAAAACTTTTTGGTGTA+CGG - Chr3:74438274-74438293 MsG0380015736.01.T01:intron 25.0%
!!! TATGGTTTTTTAGTTCCATT+TGG - Chr3:74436901-74436920 MsG0380015736.01.T01:intron 25.0%
!!! TCTTCTGAAATATATTCAGA+TGG + Chr3:74437814-74437833 None:intergenic 25.0%
ACTCTGACCAAAATAATCAT+TGG + Chr3:74436766-74436785 None:intergenic 30.0%
ACTGATGGTTCAATAATGAA+GGG + Chr3:74438837-74438856 None:intergenic 30.0%
ATACAAAGATGCTAGATCAA+GGG - Chr3:74438693-74438712 MsG0380015736.01.T01:intron 30.0%
TAAACATGAGACAGATACAT+TGG - Chr3:74436929-74436948 MsG0380015736.01.T01:intron 30.0%
TAGTTAACGAGATTCAGTTA+AGG + Chr3:74436817-74436836 None:intergenic 30.0%
TATACAAAGATGCTAGATCA+AGG - Chr3:74438692-74438711 MsG0380015736.01.T01:intron 30.0%
TATCCTTATCTTTGATTCCA+AGG - Chr3:74437213-74437232 MsG0380015736.01.T01:CDS 30.0%
TATTATTCTATTCTACCTCG+TGG - Chr3:74438545-74438564 MsG0380015736.01.T01:intron 30.0%
TCATATAAAAACAACCTCAC+AGG + Chr3:74437020-74437039 None:intergenic 30.0%
TCATCAACAATTTGGATAAG+TGG + Chr3:74436549-74436568 None:intergenic 30.0%
TCATTAACAAAATTCCTGTG+AGG - Chr3:74437003-74437022 MsG0380015736.01.T01:CDS 30.0%
TGTGACACTTAATATGACAT+AGG + Chr3:74439100-74439119 None:intergenic 30.0%
TTGTACTCAAAACTACAACA+TGG - Chr3:74439446-74439465 MsG0380015736.01.T01:CDS 30.0%
TTGTAGTTCCATCTATAAGT+GGG + Chr3:74438465-74438484 None:intergenic 30.0%
TTTGTAGTTCCATCTATAAG+TGG + Chr3:74438466-74438485 None:intergenic 30.0%
! ACACAACAGGGAAATATTTT+TGG + Chr3:74436351-74436370 None:intergenic 30.0%
! ATGTAATTTAAAGCTGTCCT+TGG + Chr3:74437233-74437252 None:intergenic 30.0%
! GATTTGATAAATGGAAGCAT+AGG - Chr3:74439614-74439633 MsG0380015736.01.T01:exon 30.0%
! TTTTTGCCTTTTCAGTAACA+TGG - Chr3:74436374-74436393 MsG0380015736.01.T01:CDS 30.0%
!! ACTGAATTTCTTGTTGTTGA+AGG + Chr3:74439370-74439389 None:intergenic 30.0%
!! ATATCATGTGAAGAGCATTT+GGG + Chr3:74438939-74438958 None:intergenic 30.0%
!! TACAAAACTTTTTGGTGTAC+GGG - Chr3:74438275-74438294 MsG0380015736.01.T01:intron 30.0%
!!! GACATCAATTTGGTTCTTTT+GGG - Chr3:74439406-74439425 MsG0380015736.01.T01:CDS 30.0%
!!! TGACATCAATTTGGTTCTTT+TGG - Chr3:74439405-74439424 MsG0380015736.01.T01:CDS 30.0%
AAATAGCCCTGCAAAAAGAA+CGG + Chr3:74438056-74438075 None:intergenic 35.0%
AACCAAATTGATGTCACGTT+TGG + Chr3:74439401-74439420 None:intergenic 35.0%
AAGCATAGGATTGAACAAGA+TGG - Chr3:74439628-74439647 MsG0380015736.01.T01:five_prime_UTR 35.0%
AAGGCAAAAACAAACACAAC+AGG + Chr3:74436364-74436383 None:intergenic 35.0%
AATAACAGCAAATGCTGCTA+AGG + Chr3:74437604-74437623 None:intergenic 35.0%
AATACTTCTGATGACCCAAT+AGG + Chr3:74437880-74437899 None:intergenic 35.0%
AATTAAGGTAACGACTACGA+TGG - Chr3:74438030-74438049 MsG0380015736.01.T01:intron 35.0%
ACTCAAAACTACAACATGGT+AGG - Chr3:74439450-74439469 MsG0380015736.01.T01:CDS 35.0%
AGGCAAAAACAAACACAACA+GGG + Chr3:74436363-74436382 None:intergenic 35.0%
ATAGGATTGAACAAGATGGT+TGG - Chr3:74439632-74439651 MsG0380015736.01.T01:five_prime_UTR 35.0%
ATCGTCCTTTGCATAAATCA+AGG + Chr3:74438440-74438459 None:intergenic 35.0%
ATTACCTATCTAGCTATCCA+TGG - Chr3:74437559-74437578 MsG0380015736.01.T01:intron 35.0%
CAAATGCTCTTCACATGATA+TGG - Chr3:74438938-74438957 MsG0380015736.01.T01:intron 35.0%
CACAAATTGCAAGTCTCATA+GGG - Chr3:74437913-74437932 MsG0380015736.01.T01:intron 35.0%
CACTGATGGTTCAATAATGA+AGG + Chr3:74438838-74438857 None:intergenic 35.0%
CATGTGCATTGTTGAATTCA+AGG - Chr3:74437783-74437802 MsG0380015736.01.T01:intron 35.0%
CATTCCTATTGCTACCTATT+GGG - Chr3:74437863-74437882 MsG0380015736.01.T01:intron 35.0%
CTCTTCACATGATATGGATT+GGG - Chr3:74438944-74438963 MsG0380015736.01.T01:intron 35.0%
CTTGAATTCAACAATGCACA+TGG + Chr3:74437785-74437804 None:intergenic 35.0%
GACTTATACACATGACAAAC+AGG - Chr3:74438583-74438602 MsG0380015736.01.T01:intron 35.0%
TAACAGAATGCAGCTAAAGA+AGG - Chr3:74436431-74436450 MsG0380015736.01.T01:CDS 35.0%
TACAAAGATGCTAGATCAAG+GGG - Chr3:74438694-74438713 MsG0380015736.01.T01:intron 35.0%
TAGGATTGAACAAGATGGTT+GGG - Chr3:74439633-74439652 MsG0380015736.01.T01:five_prime_UTR 35.0%
TCACAAATTGCAAGTCTCAT+AGG - Chr3:74437912-74437931 MsG0380015736.01.T01:intron 35.0%
TGTAGTTCCATCTATAAGTG+GGG + Chr3:74438464-74438483 None:intergenic 35.0%
TGTCCTTGGAATCAAAGATA+AGG + Chr3:74437219-74437238 None:intergenic 35.0%
TGTTAATGTCGAAAAAGGAG+TGG - Chr3:74437149-74437168 MsG0380015736.01.T01:intron 35.0%
TTCCAAACGTGACATCAATT+TGG - Chr3:74439396-74439415 MsG0380015736.01.T01:intron 35.0%
! ATATGAGGCATTTCTCTATC+TGG - Chr3:74438366-74438385 MsG0380015736.01.T01:intron 35.0%
! TCTTCACATGATATGGATTG+GGG - Chr3:74438945-74438964 MsG0380015736.01.T01:intron 35.0%
! TTTTGGTCAGAGTTCAAACT+CGG - Chr3:74436773-74436792 MsG0380015736.01.T01:CDS 35.0%
!! AGCTTTAAGAAAGAATGCAG+TGG - Chr3:74437364-74437383 MsG0380015736.01.T01:intron 35.0%
!! AGGTTAGTCTTGAAGTACTA+AGG - Chr3:74438603-74438622 MsG0380015736.01.T01:intron 35.0%
!! CATATCATGTGAAGAGCATT+TGG + Chr3:74438940-74438959 None:intergenic 35.0%
!! TTAGTCTTGAAGTACTAAGG+AGG - Chr3:74438606-74438625 MsG0380015736.01.T01:intron 35.0%
!!! AGCAGCATTTGCTGTTATTT+TGG - Chr3:74437605-74437624 MsG0380015736.01.T01:intron 35.0%
!!! GCTGTTATTTTGGAGAGTTT+TGG - Chr3:74437615-74437634 MsG0380015736.01.T01:intron 35.0%
!!! GTTTTTTAGTTCCATTTGGC+AGG - Chr3:74436905-74436924 MsG0380015736.01.T01:intron 35.0%
AAACACACTTGCCAAATCTG+TGG - Chr3:74437661-74437680 MsG0380015736.01.T01:intron 40.0%
ACAGAATTCTCTGCAATGTC+AGG + Chr3:74437703-74437722 None:intergenic 40.0%
AGACACACTATGAGATGGTA+TGG - Chr3:74438719-74438738 MsG0380015736.01.T01:intron 40.0%
AGATGCACATGCATCAACAA+TGG - Chr3:74437527-74437546 MsG0380015736.01.T01:intron 40.0%
ATCTGGTACATCTTTCGAGA+CGG - Chr3:74438383-74438402 MsG0380015736.01.T01:intron 40.0%
ATCTTCAATCAACAATGCCG+TGG - Chr3:74438797-74438816 MsG0380015736.01.T01:intron 40.0%
ATGACCCAATAGGTAGCAAT+AGG + Chr3:74437870-74437889 None:intergenic 40.0%
CAATGAACTCACCTATAGCT+TGG + Chr3:74438169-74438188 None:intergenic 40.0%
CACTAGCCATGTTACTGAAA+AGG + Chr3:74436383-74436402 None:intergenic 40.0%
CCAATAGGTAGCAATAGGAA+TGG + Chr3:74437865-74437884 None:intergenic 40.0%
CCATTCCTATTGCTACCTAT+TGG - Chr3:74437862-74437881 MsG0380015736.01.T01:intron 40.0%
CTCATGTTTAACCTGCCAAA+TGG + Chr3:74436919-74436938 None:intergenic 40.0%
GAGAGCCTTGATTTATGCAA+AGG - Chr3:74438432-74438451 MsG0380015736.01.T01:intron 40.0%
GAGTTAGAGATGGTCTATGT+AGG - Chr3:74439303-74439322 MsG0380015736.01.T01:intron 40.0%
GCAATGTCAGGTAGTTGTTA+AGG + Chr3:74437691-74437710 None:intergenic 40.0%
GCGATTGTTAAAGATGTACC+AGG + Chr3:74438317-74438336 None:intergenic 40.0%
GCTCTTCACATGATATGGAT+TGG - Chr3:74438943-74438962 MsG0380015736.01.T01:intron 40.0%
TACAGAATATTGCCCAACCT+CGG + Chr3:74439538-74439557 None:intergenic 40.0%
TCCTTCTGTCATCTACAACA+AGG + Chr3:74439195-74439214 None:intergenic 40.0%
TGCTCTTTCTACCAAGCTAT+AGG - Chr3:74438155-74438174 MsG0380015736.01.T01:CDS 40.0%
TGGATCAAGAGCTACTAGAA+TGG + Chr3:74438900-74438919 None:intergenic 40.0%
TGTTAGAAGCAGCTGATAAC+TGG + Chr3:74436416-74436435 None:intergenic 40.0%
TTCTCATCTGTACCAGAGTA+AGG + Chr3:74438342-74438361 None:intergenic 40.0%
TTCTTCTTCTCCACTTGTGA+TGG + Chr3:74438985-74439004 None:intergenic 40.0%
TTTGGCATTTCCATCACAAG+TGG - Chr3:74438972-74438991 MsG0380015736.01.T01:intron 40.0%
! AACACAACTTTTGCATGCCA+TGG + Chr3:74437579-74437598 None:intergenic 40.0%
! ACAGATGAGAAGAGCATATG+AGG - Chr3:74438351-74438370 MsG0380015736.01.T01:intron 40.0%
! GGACTGAACTTGTTTCATCA+TGG + Chr3:74436490-74436509 None:intergenic 40.0%
! TAGCAACTTCGAGTTAGAGA+TGG - Chr3:74439293-74439312 MsG0380015736.01.T01:intron 40.0%
! TCCTTGTTGTAGATGACAGA+AGG - Chr3:74439191-74439210 MsG0380015736.01.T01:intron 40.0%
!! AATCATTGCTCGAAGTGTTG+AGG - Chr3:74436581-74436600 MsG0380015736.01.T01:intron 40.0%
!! ATCATTGCTCGAAGTGTTGA+GGG - Chr3:74436582-74436601 MsG0380015736.01.T01:intron 40.0%
!! CATTTGGTCTTAATCCTCCT+AGG - Chr3:74436712-74436731 MsG0380015736.01.T01:CDS 40.0%
!! CTTCACATGATATGGATTGG+GGG - Chr3:74438946-74438965 MsG0380015736.01.T01:intron 40.0%
!! GATATGGATTGGGGGAAATT+TGG - Chr3:74438954-74438973 MsG0380015736.01.T01:intron 40.0%
!! TTGGGTCATCAGAAGTATTG+TGG - Chr3:74437881-74437900 MsG0380015736.01.T01:intron 40.0%
AGTGACTTTGTGAGCAAGAC+TGG + Chr3:74438106-74438125 None:intergenic 45.0%
ATGACTGTGCTTAGCTTCGA+CGG - Chr3:74439506-74439525 MsG0380015736.01.T01:CDS 45.0%
ATTCGATATGCTTGATGCGC+TGG - Chr3:74436974-74436993 MsG0380015736.01.T01:CDS 45.0%
CAGCTATCATGCAAATGCTC+TGG - Chr3:74437498-74437517 MsG0380015736.01.T01:intron 45.0%
CAGTTGTGGACAAAGCAACA+TGG - Chr3:74438746-74438765 MsG0380015736.01.T01:intron 45.0%
CATGCCATGGATAGCTAGAT+AGG + Chr3:74437566-74437585 None:intergenic 45.0%
CCAAATCTCTTGCTTCCACA+AGG + Chr3:74439009-74439028 None:intergenic 45.0%
CCTTGTGGAAGCAAGAGATT+TGG - Chr3:74439006-74439025 MsG0380015736.01.T01:CDS 45.0%
CTAGTAGCTCTTGATCCACA+AGG - Chr3:74438902-74438921 MsG0380015736.01.T01:intron 45.0%
CTATCATGCAAATGCTCTGG+AGG - Chr3:74437501-74437520 MsG0380015736.01.T01:intron 45.0%
CTGTAGAGGACCATCTGAAA+TGG - Chr3:74439553-74439572 MsG0380015736.01.T01:CDS 45.0%
GACGATTCCCCACTTATAGA+TGG - Chr3:74438454-74438473 MsG0380015736.01.T01:intron 45.0%
GATGGTATGGATTCCAGTTG+TGG - Chr3:74438732-74438751 MsG0380015736.01.T01:intron 45.0%
GCTTGGTAGAAAGAGCAAGT+TGG + Chr3:74438152-74438171 None:intergenic 45.0%
GGTCAACTTTACTTAGCCCT+CGG + Chr3:74436745-74436764 None:intergenic 45.0%
GGTTGGGCAATATTCTGTAG+AGG - Chr3:74439539-74439558 MsG0380015736.01.T01:CDS 45.0%
GTGGAGAAGAAGAATCCTTG+TGG - Chr3:74438991-74439010 MsG0380015736.01.T01:intron 45.0%
GTTAAGGAGAGCCACAGATT+TGG + Chr3:74437675-74437694 None:intergenic 45.0%
TAACAATCGCGTCCTTACTC+TGG - Chr3:74438327-74438346 MsG0380015736.01.T01:intron 45.0%
TGCCATCAACAAGCAACTCA+AGG + Chr3:74439034-74439053 None:intergenic 45.0%
TTAGCCCTAGCCATTTCAGA+TGG + Chr3:74439566-74439585 None:intergenic 45.0%
TTTCATCAGCCTCAGAAGCA+TGG - Chr3:74438073-74438092 MsG0380015736.01.T01:intron 45.0%
! AGCCTTGAGTTGCTTGTTGA+TGG - Chr3:74439029-74439048 MsG0380015736.01.T01:CDS 45.0%
! AGGACCATCTGAAATGGCTA+GGG - Chr3:74439559-74439578 MsG0380015736.01.T01:CDS 45.0%
! ATCGATTTCACGACCATCAG+GGG + Chr3:74436520-74436539 None:intergenic 45.0%
! GCATGCATGCATGTGTCATT+TGG - Chr3:74436696-74436715 MsG0380015736.01.T01:intron 45.0%
! TCTGAAATGGCTAGGGCTAA+AGG - Chr3:74439566-74439585 MsG0380015736.01.T01:CDS 45.0%
! TGATCCTGCACTTTTGGACT+GGG - Chr3:74439055-74439074 MsG0380015736.01.T01:CDS 45.0%
! TTCATCAGCCTCAGAAGCAT+GGG - Chr3:74438074-74438093 MsG0380015736.01.T01:intron 45.0%
! TTGATCCTGCACTTTTGGAC+TGG - Chr3:74439054-74439073 MsG0380015736.01.T01:CDS 45.0%
!! ACATCGATTTCACGACCATC+AGG + Chr3:74436522-74436541 None:intergenic 45.0%
!! AGCCTTCACAAGCTTGTTGA+GGG + Chr3:74437749-74437768 None:intergenic 45.0%
!! CATCGATTTCACGACCATCA+GGG + Chr3:74436521-74436540 None:intergenic 45.0%
!! CATGTTGCTTTGTCCACAAC+TGG + Chr3:74438748-74438767 None:intergenic 45.0%
!! TCATGTGAAGAGCATTTGGG+AGG + Chr3:74438936-74438955 None:intergenic 45.0%
!! TTGTTGAGGGCATCAAACTG+TGG + Chr3:74437736-74437755 None:intergenic 45.0%
!!! TGGCATTGATCCTGCACTTT+TGG - Chr3:74439049-74439068 MsG0380015736.01.T01:CDS 45.0%
!!! TTTTTTTTAAATGATAATTA+AGG - Chr3:74438015-74438034 MsG0380015736.01.T01:intron 5.0%
ATGGAGGCGAAGATCTAGAG+TGG - Chr3:74439231-74439250 MsG0380015736.01.T01:intron 50.0%
GCACTTGCAACAGACATAGG+AGG + Chr3:74437841-74437860 None:intergenic 50.0%
GGAAGATAGCGTCTCTAACC+TGG - Chr3:74438296-74438315 MsG0380015736.01.T01:intron 50.0%
GGATAACTCCCATGCTTCTG+AGG + Chr3:74438085-74438104 None:intergenic 50.0%
GGGTGAGACACACTATGAGA+TGG - Chr3:74438714-74438733 MsG0380015736.01.T01:intron 50.0%
TGCCCTCAACAAGCTTGTGA+AGG - Chr3:74437744-74437763 MsG0380015736.01.T01:intron 50.0%
TGGGAGGACAATCTTCCTTG+TGG + Chr3:74438920-74438939 None:intergenic 50.0%
! ACGATGGCCGTTCTTTTTGC+AGG - Chr3:74438046-74438065 MsG0380015736.01.T01:intron 50.0%
! CATACCCAGTCCAAAAGTGC+AGG + Chr3:74439062-74439081 None:intergenic 50.0%
! CGATGGCCGTTCTTTTTGCA+GGG - Chr3:74438047-74438066 MsG0380015736.01.T01:intron 50.0%
! GAGGACCATCTGAAATGGCT+AGG - Chr3:74439558-74439577 MsG0380015736.01.T01:CDS 50.0%
! GGAGCACTTGCAACAGACAT+AGG + Chr3:74437844-74437863 None:intergenic 50.0%
!! CAGCCTTCACAAGCTTGTTG+AGG + Chr3:74437750-74437769 None:intergenic 50.0%
GAAGCAGCTGATAACTGGCG+AGG + Chr3:74436411-74436430 None:intergenic 55.0%
AGCTTCGACGGTAGTGACCG+AGG - Chr3:74439518-74439537 MsG0380015736.01.T01:CDS 60.0%
CAGTCCACTCATGCCCCTGA+TGG - Chr3:74436504-74436523 MsG0380015736.01.T01:CDS 60.0%
CCTCCTAGGAGTTCAGCCGA+GGG - Chr3:74436726-74436745 MsG0380015736.01.T01:CDS 60.0%
CGACGGTAGTGACCGAGGTT+GGG - Chr3:74439523-74439542 MsG0380015736.01.T01:CDS 60.0%
GGGTCACGAACACTGTGCCA+CGG + Chr3:74438817-74438836 None:intergenic 60.0%
TAGCCCTCGGCTGAACTCCT+AGG + Chr3:74436732-74436751 None:intergenic 60.0%
TCCTCCTAGGAGTTCAGCCG+AGG - Chr3:74436725-74436744 MsG0380015736.01.T01:CDS 60.0%
TCGACGGTAGTGACCGAGGT+TGG - Chr3:74439522-74439541 MsG0380015736.01.T01:CDS 60.0%
! ACGACCATCAGGGGCATGAG+TGG + Chr3:74436511-74436530 None:intergenic 60.0%
CCCTCGGCTGAACTCCTAGG+AGG + Chr3:74436729-74436748 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 74436345 74439667 74436345 ID=MsG0380015736.01;Name=MsG0380015736.01
Chr3 mRNA 74436345 74439667 74436345 ID=MsG0380015736.01.T01;Parent=MsG0380015736.01;Name=MsG0380015736.01.T01;_AED=0.35;_eAED=0.46;_QI=48|0.16|0.14|1|1|1|7|0|373
Chr3 exon 74436345 74436574 74436345 ID=MsG0380015736.01.T01:exon:14327;Parent=MsG0380015736.01.T01
Chr3 exon 74436708 74436852 74436708 ID=MsG0380015736.01.T01:exon:14326;Parent=MsG0380015736.01.T01
Chr3 exon 74436936 74437103 74436936 ID=MsG0380015736.01.T01:exon:14325;Parent=MsG0380015736.01.T01
Chr3 exon 74437187 74437304 74437187 ID=MsG0380015736.01.T01:exon:14324;Parent=MsG0380015736.01.T01
Chr3 exon 74438128 74438273 74438128 ID=MsG0380015736.01.T01:exon:14323;Parent=MsG0380015736.01.T01
Chr3 exon 74438993 74439085 74438993 ID=MsG0380015736.01.T01:exon:14322;Parent=MsG0380015736.01.T01
Chr3 exon 74439398 74439667 74439398 ID=MsG0380015736.01.T01:exon:14321;Parent=MsG0380015736.01.T01
Chr3 five_prime_UTR 74439620 74439667 74439620 ID=MsG0380015736.01.T01:five_prime_utr;Parent=MsG0380015736.01.T01
Chr3 CDS 74439398 74439619 74439398 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74438993 74439085 74438993 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74438128 74438273 74438128 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74437187 74437304 74437187 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74436936 74437103 74436936 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74436708 74436852 74436708 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Chr3 CDS 74436345 74436574 74436345 ID=MsG0380015736.01.T01:cds;Parent=MsG0380015736.01.T01
Gene Sequence

>MsG0380015736.01.T01

ATGGCTAGTGCTAATCCTCGCCAGTTATCAGCTGCTTCTAACAGAATGCAGCTAAAGAAGGAGAGACGCATGTTCTCTACTTCTGATGATAGTGCCATGATGAAACAAGTTCAGTCCACTCATGCCCCTGATGGTCGTGAAATCGATGTCAAACCACTTATCCAAATTGTTGATGAAATCTTGATTCAAATCATTGCTCGAAGTGTTGAGGGTCATGAACATGTTAAACATGAGACAGATACATTGGAAACTACTGCTGCTTTAGCTGAATTCGATATGCTTGATGCGCTGGCTTTTATCATTAACAAAATTCCTTTTGATGCCCTCAACAAGCTTGTGAAGGCTGCACTTGATGTTACCATGTGCATTGTTGAATTCAAGGATTTGCCATCTGAATATATTTCAGAAGATGCTCCTCCTATGTCTGTTGCAAGTGCTCCATTCCTATTGCTACCTATTGGATCAAGGGGTGAGACACACTATGAGATGGTATGGATTCCAGTTGTGGACAAAGCAACATGGAATGAAGTGAATAAGCAAAAGTTTGAGTATCTTCAATCAACAATGCCGTGGCACAGTCTCTTGATCCACAAGGAAGATTGTCCTCCCAAATGCTCTTCACATGATATGGATTGGGGGAAATTTGGCATTTCCATCACAAGTGGAGAAGAAGAATCCTTGTGGAAGCAAGAGATTTGGAGCCTTGAGTTGCTTGTTGATGGCATTGATCCTGCACTTTTGGACTGGAATGCTGATAAAACATTTATACATTCTCTTGTCCTTGTTGTAGATGACAGAAGGAAAAATCATATGTCTATATGGAGGCGAAGATCTAGAGTGGATAGAAACATTCACAAAAACAGCAAGAACGTAGCAAGAATAGCAACTTCGAAGAGCATGTTGTACTCAAAACTACAACATGGTAGGACAGTTGAAAATGACAAGATCATGAGTGAAGTGATGACTGTGCTTAGCTTCGACGGTAGTGACCGAGGTTGGGCAATATTCTGTAGAGGACCATCTGAAATGGCTAGGGCTAAAGGTGACACTGCTTTAACAAGTTTGAGAGATTTGATAAATGGAAGCATAGGATTGAACAAGATGGTTGGGTTCCAGCATTGA
Protein sequence

>MsG0380015736.01.T01

MASANPRQLSAASNRMQLKKERRMFSTSDDSAMMKQVQSTHAPDGREIDVKPLIQIVDEILIQIIARSVEGHEHVKHETDTLETTAALAEFDMLDALAFIINKIPFDALNKLVKAALDVTMCIVEFKDLPSEYISEDAPPMSVASAPFLLLPIGSRGETHYEMVWIPVVDKATWNEVNKQKFEYLQSTMPWHSLLIHKEDCPPKCSSHDMDWGKFGISITSGEEESLWKQEIWSLELLVDGIDPALLDWNADKTFIHSLVLVVDDRRKNHMSIWRRRSRVDRNIHKNSKNVARIATSKSMLYSKLQHGRTVENDKIMSEVMTVLSFDGSDRGWAIFCRGPSEMARAKGDTALTSLRDLINGSIGLNKMVGFQH*