AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045245.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045245.01.T01 MTR_8g068560 95.556 90 4 0 1 90 1 90 2.52e-57 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045245.01.T01 AT5G65495 60.606 66 26 0 19 84 24 89 1.66e-12 58.2

Find 28 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTGAGCAAGGCGAGCTT+CGG 0.384176 8:+56355046 None:intergenic
CGGTGACCGAGACGGTTATA+GGG 0.423807 8:+56354878 None:intergenic
AAGCAGAGCTTCACGAGCTC+TGG 0.433185 8:+56355025 None:intergenic
TCTGTCATGGAAATTCTTGA+AGG 0.455301 8:-56354969 MsG0880045245.01.T01:CDS
GGGTAGGTGGTTGAAGAGAA+AGG 0.455948 8:+56354898 None:intergenic
TCGGCGACGACGGTGGATGA+TGG 0.503954 8:+56355116 None:intergenic
CTTCGGTGTCGGCAAGGCGA+AGG 0.506791 8:+56355063 None:intergenic
CACGAGCTCTGGCTTGAGCA+AGG 0.515030 8:+56355036 None:intergenic
GAGATTCTCGAGCACGGAGG+AGG 0.525180 8:+56355088 None:intergenic
ATAAGAAGCGCTTCATCTCA+AGG 0.544198 8:+56354991 None:intergenic
GAGCACGGAGGAGGAGTTGT+CGG 0.546872 8:+56355097 None:intergenic
GGAGGAGTTGTCGGCGACGA+CGG 0.548594 8:+56355106 None:intergenic
AGCGCTTCATCTCAAGGAGT+TGG 0.551794 8:+56354997 None:intergenic
GGCGAGCTTCGGTGTCGGCA+AGG 0.552662 8:+56355057 None:intergenic
GAAGCGCTTCTTATCTGTCA+TGG 0.558681 8:-56354982 MsG0880045245.01.T01:CDS
AGCAAGGCGAGCTTCGGTGT+CGG 0.570462 8:+56355052 None:intergenic
CGGTGTCGGCAAGGCGAAGG+CGG 0.570955 8:+56355066 None:intergenic
GTTGTTGTTGAAGACGGTAA+CGG 0.588305 8:+56354934 None:intergenic
AAATCAGAAGCTATGTGTTG+CGG 0.611884 8:-56355155 None:intergenic
GGAGTTGTCGGCGACGACGG+TGG 0.618650 8:+56355109 None:intergenic
ATGATGGAAACGATGGGTGT+TGG 0.622494 8:+56355132 None:intergenic
AAGGCGGAGATTCTCGAGCA+CGG 0.631274 8:+56355082 None:intergenic
CGACACGTTGTTGTTGAAGA+CGG 0.635368 8:+56354928 None:intergenic
CGAGACGGTTATAGGGTAGG+TGG 0.641134 8:+56354885 None:intergenic
ACGGTGGATGATGGAAACGA+TGG 0.661156 8:+56355125 None:intergenic
CGGTGGATGATGGAAACGAT+GGG 0.687316 8:+56355126 None:intergenic
GACCGAGACGGTTATAGGGT+AGG 0.702701 8:+56354882 None:intergenic
GCGGAGATTCTCGAGCACGG+AGG 0.751988 8:+56355085 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCTGTCATGGAAATTCTTGA+AGG - Chr8:56355067-56355086 MsG0880045245.01.T01:CDS 35.0%
! GTTGTTGTTGAAGACGGTAA+CGG + Chr8:56355105-56355124 None:intergenic 40.0%
!! ATAAGAAGCGCTTCATCTCA+AGG + Chr8:56355048-56355067 None:intergenic 40.0%
! ATGATGGAAACGATGGGTGT+TGG + Chr8:56354907-56354926 None:intergenic 45.0%
! CGACACGTTGTTGTTGAAGA+CGG + Chr8:56355111-56355130 None:intergenic 45.0%
! GAAGCGCTTCTTATCTGTCA+TGG - Chr8:56355054-56355073 MsG0880045245.01.T01:CDS 45.0%
AGCGCTTCATCTCAAGGAGT+TGG + Chr8:56355042-56355061 None:intergenic 50.0%
GGGTAGGTGGTTGAAGAGAA+AGG + Chr8:56355141-56355160 None:intergenic 50.0%
!! ACGGTGGATGATGGAAACGA+TGG + Chr8:56354914-56354933 None:intergenic 50.0%
!! CGGTGGATGATGGAAACGAT+GGG + Chr8:56354913-56354932 None:intergenic 50.0%
AAGCAGAGCTTCACGAGCTC+TGG + Chr8:56355014-56355033 None:intergenic 55.0%
AAGGCGGAGATTCTCGAGCA+CGG + Chr8:56354957-56354976 None:intergenic 55.0%
GAGATTCTCGAGCACGGAGG+AGG + Chr8:56354951-56354970 None:intergenic 60.0%
GAGCACGGAGGAGGAGTTGT+CGG + Chr8:56354942-56354961 None:intergenic 60.0%
GGCTTGAGCAAGGCGAGCTT+CGG + Chr8:56354993-56355012 None:intergenic 60.0%
! AGCAAGGCGAGCTTCGGTGT+CGG + Chr8:56354987-56355006 None:intergenic 60.0%
! CACGAGCTCTGGCTTGAGCA+AGG + Chr8:56355003-56355022 None:intergenic 60.0%
GCGGAGATTCTCGAGCACGG+AGG + Chr8:56354954-56354973 None:intergenic 65.0%
GGAGGAGTTGTCGGCGACGA+CGG + Chr8:56354933-56354952 None:intergenic 65.0%
TCGGCGACGACGGTGGATGA+TGG + Chr8:56354923-56354942 None:intergenic 65.0%
!! CTTCGGTGTCGGCAAGGCGA+AGG + Chr8:56354976-56354995 None:intergenic 65.0%
GGAGTTGTCGGCGACGACGG+TGG + Chr8:56354930-56354949 None:intergenic 70.0%
!! CGGTGTCGGCAAGGCGAAGG+CGG + Chr8:56354973-56354992 None:intergenic 70.0%
!! GGCGAGCTTCGGTGTCGGCA+AGG + Chr8:56354982-56355001 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 56354893 56355165 56354893 ID=MsG0880045245.01;Name=MsG0880045245.01
Chr8 mRNA 56354893 56355165 56354893 ID=MsG0880045245.01.T01;Parent=MsG0880045245.01;Name=MsG0880045245.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|90
Chr8 exon 56354893 56355165 56354893 ID=MsG0880045245.01.T01:exon:24261;Parent=MsG0880045245.01.T01
Chr8 CDS 56354893 56355165 56354893 ID=MsG0880045245.01.T01:cds;Parent=MsG0880045245.01.T01
Gene Sequence

>MsG0880045245.01.T01

ATGTGTTGCGGCCAACACCCATCGTTTCCATCATCCACCGTCGTCGCCGACAACTCCTCCTCCGTGCTCGAGAATCTCCGCCTTCGCCTTGCCGACACCGAAGCTCGCCTTGCTCAAGCCAGAGCTCGTGAAGCTCTGCTTGCACGCCAACTCCTTGAGATGAAGCGCTTCTTATCTGTCATGGAAATTCTTGAAGGTTATCTTAAACGCCGTTACCGTCTTCAACAACAACGTGTCGCTCGTCTCCTTTCTCTTCAACCACCTACCCTATAA
Protein sequence

>MsG0880045245.01.T01

MCCGQHPSFPSSTVVADNSSSVLENLRLRLADTEARLAQARAREALLARQLLEMKRFLSVMEILEGYLKRRYRLQQQRVARLLSLQPPTL*