AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031805.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031805.01.T01 MTR_6g033295 71.429 112 26 2 1 106 1 112 5.04e-12 58.5
MsG0680031805.01.T01 MTR_6g033295 78.218 101 16 2 1 95 1 101 6.38e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACCCGCCGCCGGGTTATC+CGG 0.293634 6:-27113253 MsG0680031805.01.T01:CDS
TGCGGTGGCGGCGGCGGTGG+TGG 0.308701 6:+27113222 None:intergenic
GGCGGTGGTCCCGGTGGTGG+TGG 0.347849 6:+27113294 None:intergenic
TGATGCGGTGGCGGCGGCGG+TGG 0.384162 6:+27113219 None:intergenic
GGTCCCGGTGGTGGTGGGTA+TGG 0.385679 6:+27113300 None:intergenic
TGAGGCGGAGCAGGAGGCGG+TGG 0.402195 6:+27113114 None:intergenic
GGCGGAGCAGGAGGCGGTGG+TGG 0.403106 6:+27113117 None:intergenic
TGGTGATGCGGTGGCGGCGG+CGG 0.408476 6:+27113216 None:intergenic
CAGCACCATCATGGTGATCC+TGG 0.420067 6:-27113063 MsG0680031805.01.T01:CDS
GCGGTGGCGGCGGCGGTGGT+GGG 0.433271 6:+27113223 None:intergenic
CCACCGTATGATTCATATCA+AGG 0.436074 6:-27113159 MsG0680031805.01.T01:CDS
TGAGGCGGTGGTCCCGGTGG+TGG 0.441469 6:+27113291 None:intergenic
TTGTGGTACTGAGGCGGAGC+AGG 0.446270 6:+27113105 None:intergenic
TACTGAGGCGGAGCAGGAGG+CGG 0.464290 6:+27113111 None:intergenic
GGTGGAGGATAATTACTCAT+TGG 0.464871 6:+27113446 None:intergenic
GCGGTGGTCCCGGTGGTGGT+GGG 0.468822 6:+27113295 None:intergenic
TGTTGATGAGGCGGTGGTCC+CGG 0.476188 6:+27113285 None:intergenic
GTTGTTCCTTCCTCCGTGGA+TGG 0.478346 6:-27113038 MsG0680031805.01.T01:intron
GGCGGTGGTGGGAAGCCTCC+CGG 0.478520 6:+27113234 None:intergenic
TACCCGCCGCCGGGTTATCC+GGG 0.485088 6:-27113252 MsG0680031805.01.T01:CDS
ATATGAATCATACGGTGGAC+CGG 0.489356 6:+27113164 None:intergenic
TATGAATCATACGGTGGACC+GGG 0.494665 6:+27113165 None:intergenic
TCACGAAGGCTACCCGCCGC+CGG 0.499549 6:-27113262 MsG0680031805.01.T01:CDS
CTCCCGGATAACCCGGCGGC+GGG 0.505889 6:+27113250 None:intergenic
CACGAAGGCTACCCGCCGCC+GGG 0.532612 6:-27113261 MsG0680031805.01.T01:CDS
TACGGTGGACCGGGGTGATG+CGG 0.533310 6:+27113174 None:intergenic
CGGAGGAAGGAACAACAACC+AGG 0.534624 6:+27113045 None:intergenic
ATCTCCATACCCACCACCAC+CGG 0.541477 6:-27113304 MsG0680031805.01.T01:CDS
GGTGGACCGGGGTGATGCGG+TGG 0.541946 6:+27113177 None:intergenic
GGGAAGCCTCCCGGATAACC+CGG 0.548656 6:+27113243 None:intergenic
CACATACCATCCACGGAGGA+AGG 0.550018 6:+27113032 None:intergenic
TGGTACTGAGGCGGAGCAGG+AGG 0.552034 6:+27113108 None:intergenic
GTTGATCATCAGCACCATCA+TGG 0.569251 6:-27113072 MsG0680031805.01.T01:CDS
GAAATTGAAGTACCTGGTGG+AGG 0.572975 6:+27113431 None:intergenic
CCTCCCGGATAACCCGGCGG+CGG 0.573744 6:+27113249 None:intergenic
TCTCCATACCCACCACCACC+GGG 0.573788 6:-27113303 MsG0680031805.01.T01:CDS
CACCGTATGATTCATATCAA+GGG 0.577288 6:-27113158 MsG0680031805.01.T01:CDS
TACCCTTGATATGAATCATA+CGG 0.583534 6:+27113156 None:intergenic
TCGTGATGTTGATGAGGCGG+TGG 0.588874 6:+27113279 None:intergenic
AACAACCAGGATCACCATGA+TGG 0.605913 6:+27113058 None:intergenic
GGTTGTTGTTCCTTCCTCCG+TGG 0.606362 6:-27113042 MsG0680031805.01.T01:CDS
GATCAACATGCTGGTAGTTG+TGG 0.608648 6:+27113088 None:intergenic
TTGCTGCTGTTGTGTACTAG+AGG 0.613174 6:-27110300 MsG0680031805.01.T01:CDS
TAGCCTTCGTGATGTTGATG+AGG 0.638840 6:+27113273 None:intergenic
CCGCAGCCACCGCATCACCC+CGG 0.640771 6:-27113183 MsG0680031805.01.T01:intron
CCTTCGTGATGTTGATGAGG+CGG 0.649399 6:+27113276 None:intergenic
ATGAGTAATTATCCTCCACC+AGG 0.650427 6:-27113443 MsG0680031805.01.T01:intron
TACACAACAGCAGCAAAGCG+CGG 0.654455 6:+27110307 None:intergenic
TGATGAGGCGGTGGTCCCGG+TGG 0.660213 6:+27113288 None:intergenic
GGTGCTGATGATCAACATGC+TGG 0.667336 6:+27113079 None:intergenic
TCTTCTATGAAAAGTGAACG+TGG 0.670299 6:+27113492 None:intergenic
CCGCCGCCGGGTTATCCGGG+AGG 0.680818 6:-27113249 MsG0680031805.01.T01:CDS
TGCTGGTAGTTGTGGTACTG+AGG 0.681231 6:+27113096 None:intergenic
ATGAATCATACGGTGGACCG+GGG 0.683185 6:+27113166 None:intergenic
CCTTGATATGAATCATACGG+TGG 0.688053 6:+27113159 None:intergenic
TGGTAGTTGTGGTACTGAGG+CGG 0.704398 6:+27113099 None:intergenic
CCGCCTCATCAACATCACGA+AGG 0.733794 6:-27113276 MsG0680031805.01.T01:CDS
AAGCCTCCCGGATAACCCGG+CGG 0.754419 6:+27113246 None:intergenic
AAAACACATACCATCCACGG+AGG 0.785912 6:+27113028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGATAATTAAATATTTG+TGG - Chr6:27111870-27111889 MsG0680031805.01.T01:intron 10.0%
!! ATCATTTATAGTAAAAAACA+CGG + Chr6:27111602-27111621 None:intergenic 15.0%
!! TAAATTACTTGATCTATTAA+AGG + Chr6:27111949-27111968 None:intergenic 15.0%
!!! AATTGAATTGAATTTTGTTA+GGG - Chr6:27110438-27110457 MsG0680031805.01.T01:intron 15.0%
!!! CTGAAATAAAACAATTTTAA+AGG + Chr6:27113320-27113339 None:intergenic 15.0%
!!! TCAATTTTAAAATGTAACTA+AGG + Chr6:27112982-27113001 None:intergenic 15.0%
!!! TTAGTTACATTTTAAAATTG+AGG - Chr6:27112981-27113000 MsG0680031805.01.T01:intron 15.0%
!! AAGAAAAATACTAGATACAA+GGG - Chr6:27111920-27111939 MsG0680031805.01.T01:intron 20.0%
!! ACTTAAAAATGGTTATACTA+AGG + Chr6:27111012-27111031 None:intergenic 20.0%
!! AGGATTGATAAATTTAACTA+AGG + Chr6:27112689-27112708 None:intergenic 20.0%
!! ATAGATCAAGTAATTTACTA+AGG - Chr6:27111952-27111971 MsG0680031805.01.T01:intron 20.0%
!! GCATTAACATTTCATATTAA+AGG - Chr6:27113148-27113167 MsG0680031805.01.T01:CDS 20.0%
!! TAAGAAAAATACTAGATACA+AGG - Chr6:27111919-27111938 MsG0680031805.01.T01:intron 20.0%
!! TCAATTGTTATATCATTATG+TGG + Chr6:27112090-27112109 None:intergenic 20.0%
!! TTAGTTAAATTTATCAATCC+TGG - Chr6:27112688-27112707 MsG0680031805.01.T01:intron 20.0%
!!! AACTAAGGATTCAATTTTAA+AGG + Chr6:27112967-27112986 None:intergenic 20.0%
!!! CAATCTCAATTTTAATCAAA+TGG - Chr6:27111698-27111717 MsG0680031805.01.T01:intron 20.0%
!!! CTATTTTCTCAAATTATTAC+CGG - Chr6:27112875-27112894 MsG0680031805.01.T01:intron 20.0%
!!! GAATTGAATTGAATTTTGTT+AGG - Chr6:27110437-27110456 MsG0680031805.01.T01:intron 20.0%
!!! TACATTTTTCGCTATAATAT+TGG - Chr6:27112056-27112075 MsG0680031805.01.T01:intron 20.0%
! AATAAGATTATCCATTCTTC+TGG - Chr6:27111756-27111775 MsG0680031805.01.T01:intron 25.0%
! AATTAAAACCATAAACACGA+TGG + Chr6:27113401-27113420 None:intergenic 25.0%
! ATACTATATATGTAGCACAT+AGG + Chr6:27112262-27112281 None:intergenic 25.0%
! CTAATAATCTCACCAAAATT+AGG + Chr6:27112455-27112474 None:intergenic 25.0%
! GCACTAACATTTCATATTAA+AGG - Chr6:27112795-27112814 MsG0680031805.01.T01:intron 25.0%
! TATAACAATTGAATAAGCAG+AGG - Chr6:27112098-27112117 MsG0680031805.01.T01:intron 25.0%
! TTATTTGACTACCAGAATTT+GGG - Chr6:27111088-27111107 MsG0680031805.01.T01:intron 25.0%
!! AATTGAATTTTGTTAGGGTT+TGG - Chr6:27110443-27110462 MsG0680031805.01.T01:intron 25.0%
!! TTCAAAGTAACTGACTTAAT+GGG + Chr6:27110854-27110873 None:intergenic 25.0%
!! TTTCAAAGTAACTGACTTAA+TGG + Chr6:27110855-27110874 None:intergenic 25.0%
!! TTTTATTGTCCTCCTAATTT+TGG - Chr6:27112440-27112459 MsG0680031805.01.T01:intron 25.0%
!!! ATGGATCAATACTTTTTCTT+TGG - Chr6:27112416-27112435 MsG0680031805.01.T01:intron 25.0%
!!! CTAATTTTGGTGAGATTATT+AGG - Chr6:27112453-27112472 MsG0680031805.01.T01:intron 25.0%
!!! TATCAAGGGTATTTTGATAA+CGG - Chr6:27110626-27110645 MsG0680031805.01.T01:intron 25.0%
AATTACCATGTAACCAAGTT+GGG + Chr6:27112715-27112734 None:intergenic 30.0%
AGACAGAGATAATCTTATGA+AGG + Chr6:27111807-27111826 None:intergenic 30.0%
AGATGACACATCATTAAACT+AGG - Chr6:27112936-27112955 MsG0680031805.01.T01:intron 30.0%
ATAATCTCACCAAAATTAGG+AGG + Chr6:27112452-27112471 None:intergenic 30.0%
ATATATGTAGCACATAGGTT+GGG + Chr6:27112257-27112276 None:intergenic 30.0%
ATTTAGCAAACAGTTCAGTT+TGG - Chr6:27110918-27110937 MsG0680031805.01.T01:intron 30.0%
CATGCATACTCACTTAAAAA+TGG + Chr6:27111023-27111042 None:intergenic 30.0%
CTTATTTGACTACCAGAATT+TGG - Chr6:27111087-27111106 MsG0680031805.01.T01:intron 30.0%
GACAGAGATAATCTTATGAA+GGG + Chr6:27111806-27111825 None:intergenic 30.0%
TAAGTGAGTATGCATGTATA+GGG - Chr6:27111027-27111046 MsG0680031805.01.T01:intron 30.0%
TACCCTTGATATGAATCATA+CGG + Chr6:27110617-27110636 None:intergenic 30.0%
TATATATGTAGCACATAGGT+TGG + Chr6:27112258-27112277 None:intergenic 30.0%
TATATCATTATGTGGTTCGA+AGG + Chr6:27112082-27112101 None:intergenic 30.0%
TCTTAAATTATTACTGCCAG+TGG - Chr6:27111363-27111382 MsG0680031805.01.T01:intron 30.0%
TTAAGTGAGTATGCATGTAT+AGG - Chr6:27111026-27111045 MsG0680031805.01.T01:intron 30.0%
TTAATGATGTGTCATCTATG+AGG + Chr6:27112933-27112952 None:intergenic 30.0%
! AAACTGAACCTAAAGGTTTT+AGG + Chr6:27110987-27111006 None:intergenic 30.0%
! ATTGTGTTTCTCCTTTTAAG+CGG - Chr6:27112219-27112238 MsG0680031805.01.T01:intron 30.0%
! TCAAAGTAACTGACTTAATG+GGG + Chr6:27110853-27110872 None:intergenic 30.0%
! TTTACTAAGGCAGTTTTTGA+TGG - Chr6:27111965-27111984 MsG0680031805.01.T01:intron 30.0%
AATGTACGAGTTCTGTGTAT+TGG - Chr6:27112012-27112031 MsG0680031805.01.T01:intron 35.0%
AATTGTTAATGTGTTCGCCT+TGG + Chr6:27112662-27112681 None:intergenic 35.0%
ACTAGTACCAAACTAAGAAG+CGG - Chr6:27112283-27112302 MsG0680031805.01.T01:intron 35.0%
AGAAAAACACATACCATCCA+CGG + Chr6:27110748-27110767 None:intergenic 35.0%
AGCTGAAAATGTAACCAAGT+TGG + Chr6:27113055-27113074 None:intergenic 35.0%
AGGACTGCTAATTAAAACCA+TGG - Chr6:27111457-27111476 MsG0680031805.01.T01:intron 35.0%
ATGTACGAGTTCTGTGTATT+GGG - Chr6:27112013-27112032 MsG0680031805.01.T01:intron 35.0%
ATGTTTACCGCTTCTTAGTT+TGG + Chr6:27112293-27112312 None:intergenic 35.0%
ATTGTTAATGTGTTCGCCTT+GGG + Chr6:27112661-27112680 None:intergenic 35.0%
CAACTTGGTTACATGGTAAT+TGG - Chr6:27112714-27112733 MsG0680031805.01.T01:intron 35.0%
CAATTACCATGTAACCAAGT+TGG + Chr6:27112716-27112735 None:intergenic 35.0%
CACCGTATGATTCATATCAA+GGG - Chr6:27110612-27110631 MsG0680031805.01.T01:intron 35.0%
CTGTCGTATGCAATATTTGA+TGG - Chr6:27112397-27112416 MsG0680031805.01.T01:intron 35.0%
GCTGAAAATGTAACCAAGTT+GGG + Chr6:27113054-27113073 None:intergenic 35.0%
GGACTGCTAATTAAAACCAT+GGG - Chr6:27111458-27111477 MsG0680031805.01.T01:intron 35.0%
TAAATTATTACTGCCAGTGG+CGG - Chr6:27111366-27111385 MsG0680031805.01.T01:intron 35.0%
TAAGGCTAAACTGAACCTAA+AGG + Chr6:27110994-27111013 None:intergenic 35.0%
TATCCATTCTTCTGGTAGAA+TGG - Chr6:27111764-27111783 MsG0680031805.01.T01:intron 35.0%
TATCTCTGTCTCTGATAGAA+TGG - Chr6:27111816-27111835 MsG0680031805.01.T01:intron 35.0%
TCATACTTCATAGCTTAACC+AGG - Chr6:27111437-27111456 MsG0680031805.01.T01:intron 35.0%
TCCAATTCATGTCTTATCTC+AGG + Chr6:27112338-27112357 None:intergenic 35.0%
TGCACAACTAAGTTATGTGT+TGG - Chr6:27112481-27112500 MsG0680031805.01.T01:intron 35.0%
TTAAAAGCAACCACATGTGT+CGG + Chr6:27113203-27113222 None:intergenic 35.0%
TTCGCATATTATGAAGAGCA+GGG + Chr6:27112749-27112768 None:intergenic 35.0%
TTTCGCATATTATGAAGAGC+AGG + Chr6:27112750-27112769 None:intergenic 35.0%
TTTGGTGAGATTATTAGGCA+AGG - Chr6:27112458-27112477 MsG0680031805.01.T01:intron 35.0%
! ACTTGGTTACATTTTCAGCT+TGG - Chr6:27113055-27113074 MsG0680031805.01.T01:CDS 35.0%
! GAATCAACTACTGGTTGATT+AGG + Chr6:27112508-27112527 None:intergenic 35.0%
! GATAGAGAAATTGAAGTACC+TGG + Chr6:27110348-27110367 None:intergenic 35.0%
! GTTACATTTTCAGCTTGGAT+TGG - Chr6:27113060-27113079 MsG0680031805.01.T01:CDS 35.0%
! TTGTGTTTCTCCTTTTAAGC+GGG - Chr6:27112220-27112239 MsG0680031805.01.T01:intron 35.0%
! TTTCATATTAAAGGCGCATC+CGG - Chr6:27113157-27113176 MsG0680031805.01.T01:CDS 35.0%
!! TTTCAGCTTGGATTGGTAAT+TGG - Chr6:27113067-27113086 MsG0680031805.01.T01:CDS 35.0%
!!! AACCTAAAGGTTTTAGGTAG+GGG + Chr6:27110981-27111000 None:intergenic 35.0%
!!! ATGGTTTTAATTAGCAGTCC+TGG + Chr6:27111458-27111477 None:intergenic 35.0%
!!! GAACCTAAAGGTTTTAGGTA+GGG + Chr6:27110982-27111001 None:intergenic 35.0%
!!! TGAACCTAAAGGTTTTAGGT+AGG + Chr6:27110983-27111002 None:intergenic 35.0%
AAATTATACGTGCGTGTGAG+AGG - Chr6:27111839-27111858 MsG0680031805.01.T01:intron 40.0%
AACAACCGAATTGTTGTGTC+AGG - Chr6:27111566-27111585 MsG0680031805.01.T01:intron 40.0%
AATTATACGTGCGTGTGAGA+GGG - Chr6:27111840-27111859 MsG0680031805.01.T01:intron 40.0%
ATATGAATCATACGGTGGAC+CGG + Chr6:27110609-27110628 None:intergenic 40.0%
ATGAGTAATTATCCTCCACC+AGG - Chr6:27110327-27110346 MsG0680031805.01.T01:intron 40.0%
CAAGTGTGGCTGCAAATATA+AGG - Chr6:27111139-27111158 MsG0680031805.01.T01:intron 40.0%
CAGTTGCAAACTGCGATTTA+TGG + Chr6:27111119-27111138 None:intergenic 40.0%
CCACCGTATGATTCATATCA+AGG - Chr6:27110611-27110630 MsG0680031805.01.T01:intron 40.0%
CCTTGATATGAATCATACGG+TGG + Chr6:27110614-27110633 None:intergenic 40.0%
CTACAGTTTACCCGCTTAAA+AGG + Chr6:27112233-27112252 None:intergenic 40.0%
GAGTTTGTATGAGTGAGCAT+TGG + Chr6:27111735-27111754 None:intergenic 40.0%
GCAAACAGTTCAGTTTGGTT+CGG - Chr6:27110923-27110942 MsG0680031805.01.T01:intron 40.0%
GCCTGAGATAAGACATGAAT+TGG - Chr6:27112334-27112353 MsG0680031805.01.T01:intron 40.0%
GCTGCATAGTGTGTCATAAA+GGG + Chr6:27112777-27112796 None:intergenic 40.0%
GGTGGAGGATAATTACTCAT+TGG + Chr6:27110327-27110346 None:intergenic 40.0%
GTTGTGTCTAGTGTCTACTT+TGG + Chr6:27110809-27110828 None:intergenic 40.0%
TATGTGACCTCCAAGTTACA+TGG - Chr6:27111542-27111561 MsG0680031805.01.T01:intron 40.0%
TCGCATATTATGAAGAGCAG+GGG + Chr6:27112748-27112767 None:intergenic 40.0%
TGCTGCATAGTGTGTCATAA+AGG + Chr6:27112778-27112797 None:intergenic 40.0%
TGTCCATTCTACCAGAAGAA+TGG + Chr6:27111770-27111789 None:intergenic 40.0%
TTAAAAGCAACCACATGCGT+CGG + Chr6:27112850-27112869 None:intergenic 40.0%
TTATGGTGACACCCAAATTC+TGG + Chr6:27111102-27111121 None:intergenic 40.0%
TTCATGTCTTATCTCAGGCA+CGG + Chr6:27112333-27112352 None:intergenic 40.0%
! AGAGAAATTGAAGTACCTGG+TGG + Chr6:27110345-27110364 None:intergenic 40.0%
! TTTCATATTAAAGGCGCGTC+CGG - Chr6:27112804-27112823 MsG0680031805.01.T01:intron 40.0%
!! GTGAGTGTTGAATCAACTAC+TGG + Chr6:27112517-27112536 None:intergenic 40.0%
!!! GGTTTTAGAGATCTCTGCAA+CGG - Chr6:27111160-27111179 MsG0680031805.01.T01:intron 40.0%
!!! GTTTTAGAGATCTCTGCAAC+GGG - Chr6:27111161-27111180 MsG0680031805.01.T01:intron 40.0%
!!! TAAAGGTTTTAGGTAGGGGT+GGG + Chr6:27110977-27110996 None:intergenic 40.0%
AAAACACATACCATCCACGG+AGG + Chr6:27110745-27110764 None:intergenic 45.0%
AACAACCAGGATCACCATGA+TGG + Chr6:27110715-27110734 None:intergenic 45.0%
ACAAACAACGTCTCTGCTTG+CGG + Chr6:27111508-27111527 None:intergenic 45.0%
CACCCCTACCTAAAACCTTT+AGG - Chr6:27110976-27110995 MsG0680031805.01.T01:intron 45.0%
CCAAGCTGCAGTGTAAATGA+AGG + Chr6:27113443-27113462 None:intergenic 45.0%
CCTTCATTTACACTGCAGCT+TGG - Chr6:27113440-27113459 MsG0680031805.01.T01:intron 45.0%
GATCAACATGCTGGTAGTTG+TGG + Chr6:27110685-27110704 None:intergenic 45.0%
GCAGAGGATACAATTCAGTC+TGG - Chr6:27112114-27112133 MsG0680031805.01.T01:intron 45.0%
GCTTCTCTCCATCGTGTTTA+TGG - Chr6:27113390-27113409 MsG0680031805.01.T01:intron 45.0%
GTTAGTGTTTGACACCGACA+CGG - Chr6:27111304-27111323 MsG0680031805.01.T01:intron 45.0%
GTTGATCATCAGCACCATCA+TGG - Chr6:27110698-27110717 MsG0680031805.01.T01:intron 45.0%
GTTGTTGCCATGTAACTTGG+AGG + Chr6:27111552-27111571 None:intergenic 45.0%
TAGCCTTCGTGATGTTGATG+AGG + Chr6:27110500-27110519 None:intergenic 45.0%
TATCAATCCTGGACCCAACT+TGG - Chr6:27112699-27112718 MsG0680031805.01.T01:intron 45.0%
TATGAATCATACGGTGGACC+GGG + Chr6:27110608-27110627 None:intergenic 45.0%
TCGGTTGTTGCCATGTAACT+TGG + Chr6:27111555-27111574 None:intergenic 45.0%
TTCTACAATAACCAGCGAGC+AGG - Chr6:27112173-27112192 MsG0680031805.01.T01:intron 45.0%
TTGCTGCTGTTGTGTACTAG+AGG - Chr6:27113470-27113489 MsG0680031805.01.T01:five_prime_UTR 45.0%
! AATGTAACCAAGTTGGGTCC+AGG + Chr6:27113048-27113067 None:intergenic 45.0%
! GAAATTGAAGTACCTGGTGG+AGG + Chr6:27110342-27110361 None:intergenic 45.0%
!!! CTAAAGGTTTTAGGTAGGGG+TGG + Chr6:27110978-27110997 None:intergenic 45.0%
ACTAATTGTTGCCTGCTCGC+TGG + Chr6:27112187-27112206 None:intergenic 50.0%
ATGAATCATACGGTGGACCG+GGG + Chr6:27110607-27110626 None:intergenic 50.0%
CAAACAACGTCTCTGCTTGC+GGG + Chr6:27111507-27111526 None:intergenic 50.0%
CTGGACCCAACTTGGTTACA+TGG - Chr6:27112707-27112726 MsG0680031805.01.T01:intron 50.0%
CTGTGCACATTCATTGCCCA+TGG + Chr6:27111477-27111496 None:intergenic 50.0%
GCAGTTTGCAACTGCAAGTG+TGG - Chr6:27111125-27111144 MsG0680031805.01.T01:intron 50.0%
GCGAGCAGGCAACAATTAGT+TGG - Chr6:27112187-27112206 MsG0680031805.01.T01:intron 50.0%
GCGAGCCTGACACAACAATT+CGG + Chr6:27111574-27111593 None:intergenic 50.0%
TAATGTGTTCGCCTTGGGAC+TGG + Chr6:27112656-27112675 None:intergenic 50.0%
TACACAACAGCAGCAAAGCG+CGG + Chr6:27113466-27113485 None:intergenic 50.0%
TGCTGGTAGTTGTGGTACTG+AGG + Chr6:27110677-27110696 None:intergenic 50.0%
TTCGTGTCTCGTGTCTGAGT+CGG - Chr6:27111403-27111422 MsG0680031805.01.T01:intron 50.0%
! ACGCCTTCGATCAGAAGTGT+CGG + Chr6:27111266-27111285 None:intergenic 50.0%
! CATGTAACCAAGTTGGGTCC+AGG + Chr6:27112709-27112728 None:intergenic 50.0%
! CCTTCGTGATGTTGATGAGG+CGG + Chr6:27110497-27110516 None:intergenic 50.0%
! TGGTAGTTGTGGTACTGAGG+CGG + Chr6:27110674-27110693 None:intergenic 50.0%
!! CACATGTGTCACTTCCGTGT+CGG + Chr6:27111321-27111340 None:intergenic 50.0%
!! CAGAAGTGTCGGTGCTACAT+AGG + Chr6:27111255-27111274 None:intergenic 50.0%
!! GGTGCTGATGATCAACATGC+TGG + Chr6:27110694-27110713 None:intergenic 50.0%
ACGGACACTGGACATGACAC+CGG + Chr6:27112897-27112916 None:intergenic 55.0%
ATCTCCATACCCACCACCAC+CGG - Chr6:27110466-27110485 MsG0680031805.01.T01:intron 55.0%
CAACACGACACCGACACATG+TGG - Chr6:27113190-27113209 MsG0680031805.01.T01:intron 55.0%
CACATACCATCCACGGAGGA+AGG + Chr6:27110741-27110760 None:intergenic 55.0%
CAGCACCATCATGGTGATCC+TGG - Chr6:27110707-27110726 MsG0680031805.01.T01:intron 55.0%
CCGCCTCATCAACATCACGA+AGG - Chr6:27110494-27110513 MsG0680031805.01.T01:intron 55.0%
CGGAGGAAGGAACAACAACC+AGG + Chr6:27110728-27110747 None:intergenic 55.0%
GCTCACTACATCCAGTCCCA+AGG - Chr6:27112642-27112661 MsG0680031805.01.T01:intron 55.0%
GTTGTTCCTTCCTCCGTGGA+TGG - Chr6:27110732-27110751 MsG0680031805.01.T01:intron 55.0%
TCCAGTGTCCGTGTTTGCGT+CGG - Chr6:27112905-27112924 MsG0680031805.01.T01:intron 55.0%
! GCACCGACACTTCTGATCGA+AGG - Chr6:27111260-27111279 MsG0680031805.01.T01:intron 55.0%
! GGTTGTTGTTCCTTCCTCCG+TGG - Chr6:27110728-27110747 MsG0680031805.01.T01:intron 55.0%
! TCGTGATGTTGATGAGGCGG+TGG + Chr6:27110494-27110513 None:intergenic 55.0%
!! ATGAGGCACCGACGCAAACA+CGG + Chr6:27112916-27112935 None:intergenic 55.0%
!! CACATGTGTCGGTGTCGTGT+TGG + Chr6:27113192-27113211 None:intergenic 55.0%
!! GTCGTGTTGGTGTTGGACAC+CGG + Chr6:27112826-27112845 None:intergenic 55.0%
!! GTTTTAGGTAGGGGTGGGCA+TGG + Chr6:27110972-27110991 None:intergenic 55.0%
!! TGTCGGTGTCGTGTTGGTGT+TGG + Chr6:27113186-27113205 None:intergenic 55.0%
ACCAGATACGACACCGCCAC+TGG + Chr6:27111382-27111401 None:intergenic 60.0%
ACCGACGCAAACACGGACAC+TGG + Chr6:27112909-27112928 None:intergenic 60.0%
CAACACGACACCGACGCATG+TGG - Chr6:27112837-27112856 MsG0680031805.01.T01:intron 60.0%
CGTCTCTGCTTGCGGGAGTA+AGG + Chr6:27111500-27111519 None:intergenic 60.0%
GGGGTGGGCATGGTTCAGTT+CGG + Chr6:27110962-27110981 None:intergenic 60.0%
GGGTGGGCATGGTTCAGTTC+GGG + Chr6:27110961-27110980 None:intergenic 60.0%
TCTCCATACCCACCACCACC+GGG - Chr6:27110467-27110486 MsG0680031805.01.T01:intron 60.0%
! TGTTGATGAGGCGGTGGTCC+CGG + Chr6:27110488-27110507 None:intergenic 60.0%
! TTGTGGTACTGAGGCGGAGC+AGG + Chr6:27110668-27110687 None:intergenic 60.0%
!! CACATGCGTCGGTGTCGTGT+TGG + Chr6:27112839-27112858 None:intergenic 60.0%
!! CGTCGGTGTCGTGTTGGTGT+TGG + Chr6:27112833-27112852 None:intergenic 60.0%
AAGCCTCCCGGATAACCCGG+CGG + Chr6:27110527-27110546 None:intergenic 65.0%
CCACCGCATCACCACCACCA+CGG - Chr6:27110563-27110582 MsG0680031805.01.T01:intron 65.0%
GGGAAGCCTCCCGGATAACC+CGG + Chr6:27110530-27110549 None:intergenic 65.0%
TACGGTGGACCGGGGTGATG+CGG + Chr6:27110599-27110618 None:intergenic 65.0%
TACTGAGGCGGAGCAGGAGG+CGG + Chr6:27110662-27110681 None:intergenic 65.0%
! TGGTACTGAGGCGGAGCAGG+AGG + Chr6:27110665-27110684 None:intergenic 65.0%
!! GCCAGTGGCGGTGTCGTATC+TGG - Chr6:27111378-27111397 MsG0680031805.01.T01:intron 65.0%
!! GGACCGTGGTGGTGGTGATG+CGG + Chr6:27110569-27110588 None:intergenic 65.0%
!! TGGTGGTGGTGATGCGGTGG+CGG + Chr6:27110563-27110582 None:intergenic 65.0%
CTACCCGCCGCCGGGTTATC+CGG - Chr6:27110517-27110536 MsG0680031805.01.T01:intron 70.0%
TACCCGCCGCCGGGTTATCC+GGG - Chr6:27110518-27110537 MsG0680031805.01.T01:intron 70.0%
TCACGAAGGCTACCCGCCGC+CGG - Chr6:27110508-27110527 MsG0680031805.01.T01:intron 70.0%
TGATGAGGCGGTGGTCCCGG+TGG + Chr6:27110485-27110504 None:intergenic 70.0%
! TGGTGGTGATGCGGTGGCGG+CGG + Chr6:27110560-27110579 None:intergenic 70.0%
!! CCGTGGTGGTGGTGATGCGG+TGG + Chr6:27110566-27110585 None:intergenic 70.0%
!! GGTCCCGGTGGTGGTGGGTA+TGG + Chr6:27110473-27110492 None:intergenic 70.0%
CACGAAGGCTACCCGCCGCC+GGG - Chr6:27110509-27110528 MsG0680031805.01.T01:intron 75.0%
CCGCAGCCACCGCATCACCC+CGG - Chr6:27110587-27110606 MsG0680031805.01.T01:intron 75.0%
CCGGGGTGATGCGGTGGCTG+CGG + Chr6:27110590-27110609 None:intergenic 75.0%
CCTCCCGGATAACCCGGCGG+CGG + Chr6:27110524-27110543 None:intergenic 75.0%
CTCCCGGATAACCCGGCGGC+GGG + Chr6:27110523-27110542 None:intergenic 75.0%
GATGCGGTGGCTGCGGACCG+TGG + Chr6:27110583-27110602 None:intergenic 75.0%
GGTGGACCGGGGTGATGCGG+TGG + Chr6:27110596-27110615 None:intergenic 75.0%
GTGGCTGCGGACCGTGGTGG+TGG + Chr6:27110577-27110596 None:intergenic 75.0%
TGAGGCGGAGCAGGAGGCGG+TGG + Chr6:27110659-27110678 None:intergenic 75.0%
TGAGGCGGTGGTCCCGGTGG+TGG + Chr6:27110482-27110501 None:intergenic 75.0%
! GCGGTGGTCCCGGTGGTGGT+GGG + Chr6:27110478-27110497 None:intergenic 75.0%
! TGGTGATGCGGTGGCGGCGG+CGG + Chr6:27110557-27110576 None:intergenic 75.0%
!! GGCGGTGGTGGGAAGCCTCC+CGG + Chr6:27110539-27110558 None:intergenic 75.0%
! CCGCCGCCGGGTTATCCGGG+AGG - Chr6:27110521-27110540 MsG0680031805.01.T01:intron 80.0%
! GCGGTGGCTGCGGACCGTGG+TGG + Chr6:27110580-27110599 None:intergenic 80.0%
! GGCGGAGCAGGAGGCGGTGG+TGG + Chr6:27110656-27110675 None:intergenic 80.0%
!! GGCGGTGGTCCCGGTGGTGG+TGG + Chr6:27110479-27110498 None:intergenic 80.0%
!! TGATGCGGTGGCGGCGGCGG+TGG + Chr6:27110554-27110573 None:intergenic 80.0%
!! GCGGTGGCGGCGGCGGTGGT+GGG + Chr6:27110550-27110569 None:intergenic 85.0%
!! TGCGGTGGCGGCGGCGGTGG+TGG + Chr6:27110551-27110570 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr6 gene 27110280 27113512 27110280 ID=MsG0680031805.01;Name=MsG0680031805.01
Chr6 mRNA 27110280 27113512 27110280 ID=MsG0680031805.01.T01;Parent=MsG0680031805.01;Name=MsG0680031805.01.T01;_AED=0.48;_eAED=0.48;_QI=47|0.66|0.5|1|0|0|4|0|113
Chr6 exon 27110280 27110334 27110280 ID=MsG0680031805.01.T01:exon:14354;Parent=MsG0680031805.01.T01
Chr6 exon 27113039 27113199 27113039 ID=MsG0680031805.01.T01:exon:14353;Parent=MsG0680031805.01.T01
Chr6 exon 27113230 27113333 27113230 ID=MsG0680031805.01.T01:exon:14352;Parent=MsG0680031805.01.T01
Chr6 exon 27113444 27113512 27113444 ID=MsG0680031805.01.T01:exon:14351;Parent=MsG0680031805.01.T01
Chr6 five_prime_UTR 27113466 27113512 27113466 ID=MsG0680031805.01.T01:five_prime_utr;Parent=MsG0680031805.01.T01
Chr6 CDS 27113444 27113465 27113444 ID=MsG0680031805.01.T01:cds;Parent=MsG0680031805.01.T01
Chr6 CDS 27113230 27113333 27113230 ID=MsG0680031805.01.T01:cds;Parent=MsG0680031805.01.T01
Chr6 CDS 27113039 27113199 27113039 ID=MsG0680031805.01.T01:cds;Parent=MsG0680031805.01.T01
Chr6 CDS 27110280 27110334 27110280 ID=MsG0680031805.01.T01:cds;Parent=MsG0680031805.01.T01
Gene Sequence

>MsG0680031805.01.T01

ATGAGTAATTATCCTCCACCAGGGTTTGGATCTCCATACCCACCACCACCGGGACCACCGCCTCATCAACATCACGAAGGCTACCCGCCGCCGGGTTATCCGGGAGGCTTCCCACCACCGCCGCCGCCACCGCATCACCCCGGTCCACCGTATGATTCATATCAAGGGTATTTTGATAACGGATATCCACCACCGCCTCCTGCTCCGCCTCAGTACCACAACTACCAGCATGTTGATCATCAGCACCATCATGGTGATCCTGGTTGTTGTTCCTTCCTCCGTGGATGCTTGGCCGCGCTTTGCTGCTGTTGTGTACTAGAGGAATGCTGTTGCTTTTTCTGA
Protein sequence

>MsG0680031805.01.T01

MSNYPPPGFGSPYPPPPGPPPHQHHEGYPPPGYPGGFPPPPPPPHHPGPPYDSYQGYFDNGYPPPPPAPPQYHNYQHVDHQHHHGDPGCCSFLRGCLAALCCCCVLEECCCFF*