Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045399.01 | MsG0880045410.01 | 0.843896 | 1.056680e-58 | 1.677443e-55 |
| MsG0880045405.01 | MsG0880045410.01 | 0.862902 | 3.645538e-64 | 1.085134e-60 |
| MsG0880045408.01 | MsG0880045410.01 | 0.854103 | 1.538091e-61 | 3.398002e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTCCTCGCATGTTGTTTC+TGG | 0.119405 | 8:-58861328 | MsG0880045409.01.T02:five_prime_UTR |
| AATTTCACTGAGAACAAAAT+TGG | 0.222803 | 8:-58861070 | MsG0880045409.01.T02:five_prime_UTR |
| ATCTTTGATACGCCGATTAT+CGG | 0.298722 | 8:-58860947 | MsG0880045409.01.T02:five_prime_UTR |
| GCGAACCATCGTCTCCAATC+TGG | 0.357900 | 8:+58861143 | MsG0880045410.01.T01:CDS |
| TGGCCGTTGCAATTTGAGTC+AGG | 0.371578 | 8:-58861308 | MsG0880045409.01.T02:five_prime_UTR |
| TGGTGGTTGGATCGATCATT+TGG | 0.399953 | 8:+58861119 | MsG0880045410.01.T01:CDS |
| CATTGGATGAGGAATCTTCC+AGG | 0.408974 | 8:+58861238 | MsG0880045410.01.T01:CDS |
| TGCTACAATTCGTTGTTGCC+TGG | 0.410074 | 8:-58861256 | MsG0880045409.01.T02:five_prime_UTR |
| TCGACAAAGATCCCGATAAT+CGG | 0.419327 | 8:+58860935 | MsG0880045410.01.T01:CDS |
| GATAATCGGCGTATCAAAGA+TGG | 0.443540 | 8:+58860949 | MsG0880045410.01.T01:CDS |
| TCTTTGATACGCCGATTATC+GGG | 0.444431 | 8:-58860946 | MsG0880045409.01.T02:five_prime_UTR |
| GTATGTTTCAATGATGAAAC+TGG | 0.460739 | 8:-58861389 | MsG0880045409.01.T02:intron |
| CGAACCATCGTCTCCAATCT+GGG | 0.465629 | 8:+58861144 | MsG0880045410.01.T01:CDS |
| TTGGCCAGGCTCAATGCCTA+TGG | 0.471299 | 8:+58860856 | MsG0880045410.01.T01:CDS |
| TACCGTTTCCCTGGTCAACC+TGG | 0.480068 | 8:+58861354 | MsG0880045410.01.T01:CDS |
| TGCAATTTGAGTCAGGGCAG+CGG | 0.490704 | 8:-58861301 | MsG0880045409.01.T02:five_prime_UTR |
| CTCTATCGTTCCGTGGTGGT+TGG | 0.492399 | 8:+58861106 | MsG0880045410.01.T01:CDS |
| AACTGGTGATCGACATATCC+AGG | 0.502872 | 8:-58861372 | MsG0880045409.01.T02:intron |
| GCATGGTAAGGATTTAATTG+AGG | 0.507031 | 8:+58861032 | MsG0880045410.01.T01:CDS |
| TCGACATATCCAGGTTGACC+AGG | 0.507098 | 8:-58861363 | MsG0880045409.01.T02:intron |
| AGATAAGCGATTCCCCAGAT+TGG | 0.509676 | 8:-58861157 | MsG0880045409.01.T02:five_prime_UTR |
| TGTTCGCAATCAAATTGCAT+TGG | 0.510282 | 8:+58861179 | MsG0880045410.01.T01:CDS |
| GATTCAACATATGCATGGTA+AGG | 0.515364 | 8:+58861020 | MsG0880045410.01.T01:CDS |
| GGCAATCACTCAGCCTACCT+TGG | 0.522551 | 8:+58860837 | MsG0880045410.01.T01:CDS |
| ATCCAGGTTGACCAGGGAAA+CGG | 0.524632 | 8:-58861356 | MsG0880045409.01.T02:intron |
| TGCTCAGTTTACATTGGATG+AGG | 0.529316 | 8:+58861227 | MsG0880045410.01.T01:CDS |
| TCACTCAGCCTACCTTGGCC+AGG | 0.531959 | 8:+58860842 | MsG0880045410.01.T01:CDS |
| GGCCGTTGCAATTTGAGTCA+GGG | 0.534236 | 8:-58861307 | MsG0880045409.01.T02:five_prime_UTR |
| GGACGCATGTACCGTTTCCC+TGG | 0.535239 | 8:+58861345 | MsG0880045410.01.T01:CDS |
| TATGTTGAATCAAGTTAAAG+AGG | 0.535573 | 8:-58861008 | MsG0880045409.01.T02:five_prime_UTR |
| TCGTATCTGAAGGGTGCCGA+CGG | 0.543474 | 8:-58860883 | MsG0880045409.01.T02:five_prime_UTR |
| AACTTGATTCAACATATGCA+TGG | 0.547871 | 8:+58861015 | MsG0880045410.01.T01:CDS |
| TATTCCATAGGCATTGAGCC+TGG | 0.563062 | 8:-58860860 | MsG0880045409.01.T02:five_prime_UTR |
| GAAGCATGCTCAGTTTACAT+TGG | 0.564547 | 8:+58861221 | MsG0880045410.01.T01:CDS |
| TGGACTTAGTTAATCAGCAA+AGG | 0.567374 | 8:+58861199 | MsG0880045410.01.T01:CDS |
| GGGTGCCGACGGTATTCCAT+AGG | 0.579023 | 8:-58860872 | MsG0880045409.01.T02:five_prime_UTR |
| ATAGGCATTGAGCCTGGCCA+AGG | 0.589016 | 8:-58860854 | MsG0880045409.01.T02:five_prime_UTR |
| GCATTGAGCCTGGCCAAGGT+AGG | 0.597021 | 8:-58860850 | MsG0880045409.01.T02:five_prime_UTR |
| TGCCCTGACTCAAATTGCAA+CGG | 0.604605 | 8:+58861305 | MsG0880045410.01.T01:CDS |
| ATAATCGGCGTATCAAAGAT+GGG | 0.614224 | 8:+58860950 | MsG0880045410.01.T01:CDS |
| TCACAACGATCACAAAGCAA+TGG | 0.618580 | 8:+58860975 | MsG0880045410.01.T01:CDS |
| GTTGAATCAAGTTAAAGAGG+TGG | 0.634920 | 8:-58861005 | MsG0880045409.01.T02:five_prime_UTR |
| TGCAAAGCTCTATCGTTCCG+TGG | 0.658175 | 8:+58861099 | MsG0880045410.01.T01:CDS |
| CGACATATCCAGGTTGACCA+GGG | 0.659737 | 8:-58861362 | MsG0880045409.01.T02:intron |
| CAATGCCTATGGAATACCGT+CGG | 0.665714 | 8:+58860867 | MsG0880045410.01.T01:CDS |
| ATTCCCCAGATTGGAGACGA+TGG | 0.669771 | 8:-58861148 | MsG0880045409.01.T02:five_prime_UTR |
| GCAACAACGAATTGTAGCAG+AGG | 0.675734 | 8:+58861260 | MsG0880045410.01.T01:CDS |
| GAACCATCGTCTCCAATCTG+GGG | 0.682279 | 8:+58861145 | MsG0880045410.01.T01:CDS |
| ACGGCCAGAAACAACATGCG+AGG | 0.695230 | 8:+58861324 | MsG0880045410.01.T01:CDS |
| AAAGCTCTATCGTTCCGTGG+TGG | 0.701677 | 8:+58861102 | MsG0880045410.01.T01:CDS |
| AATGATCGATCCAACCACCA+CGG | 0.729748 | 8:-58861116 | MsG0880045409.01.T02:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTCACTGAGAACAAAAT+TGG | - | Chr8:58861073-58861092 | MsG0880045409.01.T02:five_prime_UTR | 25.0% |
| ! | TATGTTGAATCAAGTTAAAG+AGG | - | Chr8:58861011-58861030 | MsG0880045409.01.T02:five_prime_UTR | 25.0% |
| AACTTGATTCAACATATGCA+TGG | + | Chr8:58861015-58861034 | MsG0880045410.01.T01:CDS | 30.0% | |
| ATAATCGGCGTATCAAAGAT+GGG | + | Chr8:58860950-58860969 | MsG0880045410.01.T01:CDS | 35.0% | |
| GATTCAACATATGCATGGTA+AGG | + | Chr8:58861020-58861039 | MsG0880045410.01.T01:CDS | 35.0% | |
| GCATGGTAAGGATTTAATTG+AGG | + | Chr8:58861032-58861051 | MsG0880045410.01.T01:CDS | 35.0% | |
| GTTGAATCAAGTTAAAGAGG+TGG | - | Chr8:58861008-58861027 | MsG0880045409.01.T02:five_prime_UTR | 35.0% | |
| TGGACTTAGTTAATCAGCAA+AGG | + | Chr8:58861199-58861218 | MsG0880045410.01.T01:CDS | 35.0% | |
| TGTTCGCAATCAAATTGCAT+TGG | + | Chr8:58861179-58861198 | MsG0880045410.01.T01:CDS | 35.0% | |
| ! | ATCTTTGATACGCCGATTAT+CGG | - | Chr8:58860950-58860969 | MsG0880045409.01.T02:five_prime_UTR | 35.0% |
| ! | GCATTTTCTTCGTATCTGAA+GGG | - | Chr8:58860895-58860914 | MsG0880045409.01.T02:five_prime_UTR | 35.0% |
| GAAGCATGCTCAGTTTACAT+TGG | + | Chr8:58861221-58861240 | MsG0880045410.01.T01:CDS | 40.0% | |
| GATAATCGGCGTATCAAAGA+TGG | + | Chr8:58860949-58860968 | MsG0880045410.01.T01:CDS | 40.0% | |
| TCACAACGATCACAAAGCAA+TGG | + | Chr8:58860975-58860994 | MsG0880045410.01.T01:CDS | 40.0% | |
| TCGACAAAGATCCCGATAAT+CGG | + | Chr8:58860935-58860954 | MsG0880045410.01.T01:CDS | 40.0% | |
| TGCTCAGTTTACATTGGATG+AGG | + | Chr8:58861227-58861246 | MsG0880045410.01.T01:CDS | 40.0% | |
| ! | CGCATTTTCTTCGTATCTGA+AGG | - | Chr8:58860896-58860915 | MsG0880045409.01.T02:five_prime_UTR | 40.0% |
| ! | TCTTTGATACGCCGATTATC+GGG | - | Chr8:58860949-58860968 | MsG0880045409.01.T02:five_prime_UTR | 40.0% |
| AACTGGTGATCGACATATCC+AGG | - | Chr8:58861375-58861394 | MsG0880045409.01.T02:intron | 45.0% | |
| AATGATCGATCCAACCACCA+CGG | - | Chr8:58861119-58861138 | MsG0880045409.01.T02:five_prime_UTR | 45.0% | |
| AGATAAGCGATTCCCCAGAT+TGG | - | Chr8:58861160-58861179 | MsG0880045409.01.T02:five_prime_UTR | 45.0% | |
| CAATGCCTATGGAATACCGT+CGG | + | Chr8:58860867-58860886 | MsG0880045410.01.T01:CDS | 45.0% | |
| CATTGGATGAGGAATCTTCC+AGG | + | Chr8:58861238-58861257 | MsG0880045410.01.T01:CDS | 45.0% | |
| GCAACAACGAATTGTAGCAG+AGG | + | Chr8:58861260-58861279 | MsG0880045410.01.T01:CDS | 45.0% | |
| TGCCCTGACTCAAATTGCAA+CGG | + | Chr8:58861305-58861324 | MsG0880045410.01.T01:CDS | 45.0% | |
| TGCTACAATTCGTTGTTGCC+TGG | - | Chr8:58861259-58861278 | MsG0880045409.01.T02:five_prime_UTR | 45.0% | |
| TGGTGGTTGGATCGATCATT+TGG | + | Chr8:58861119-58861138 | MsG0880045410.01.T01:CDS | 45.0% | |
| !! | TATTCCATAGGCATTGAGCC+TGG | - | Chr8:58860863-58860882 | MsG0880045409.01.T02:five_prime_UTR | 45.0% |
| AAAGCTCTATCGTTCCGTGG+TGG | + | Chr8:58861102-58861121 | MsG0880045410.01.T01:CDS | 50.0% | |
| ATTCCCCAGATTGGAGACGA+TGG | - | Chr8:58861151-58861170 | MsG0880045409.01.T02:five_prime_UTR | 50.0% | |
| CGAACCATCGTCTCCAATCT+GGG | + | Chr8:58861144-58861163 | MsG0880045410.01.T01:CDS | 50.0% | |
| GAACCATCGTCTCCAATCTG+GGG | + | Chr8:58861145-58861164 | MsG0880045410.01.T01:CDS | 50.0% | |
| GGCCGTTGCAATTTGAGTCA+GGG | - | Chr8:58861310-58861329 | MsG0880045409.01.T02:five_prime_UTR | 50.0% | |
| TGCAAAGCTCTATCGTTCCG+TGG | + | Chr8:58861099-58861118 | MsG0880045410.01.T01:CDS | 50.0% | |
| TGCAATTTGAGTCAGGGCAG+CGG | - | Chr8:58861304-58861323 | MsG0880045409.01.T02:five_prime_UTR | 50.0% | |
| TGGCCGTTGCAATTTGAGTC+AGG | - | Chr8:58861311-58861330 | MsG0880045409.01.T02:five_prime_UTR | 50.0% | |
| ! | ATCCAGGTTGACCAGGGAAA+CGG | - | Chr8:58861359-58861378 | MsG0880045409.01.T02:intron | 50.0% |
| ! | CGACATATCCAGGTTGACCA+GGG | - | Chr8:58861365-58861384 | MsG0880045409.01.T02:intron | 50.0% |
| ! | TCGACATATCCAGGTTGACC+AGG | - | Chr8:58861366-58861385 | MsG0880045409.01.T02:intron | 50.0% |
| ACGGCCAGAAACAACATGCG+AGG | + | Chr8:58861324-58861343 | MsG0880045410.01.T01:CDS | 55.0% | |
| CTCTATCGTTCCGTGGTGGT+TGG | + | Chr8:58861106-58861125 | MsG0880045410.01.T01:CDS | 55.0% | |
| GCGAACCATCGTCTCCAATC+TGG | + | Chr8:58861143-58861162 | MsG0880045410.01.T01:CDS | 55.0% | |
| GGCAATCACTCAGCCTACCT+TGG | + | Chr8:58860837-58860856 | MsG0880045410.01.T01:CDS | 55.0% | |
| TACCGTTTCCCTGGTCAACC+TGG | + | Chr8:58861354-58861373 | MsG0880045410.01.T01:CDS | 55.0% | |
| TTGGCCAGGCTCAATGCCTA+TGG | + | Chr8:58860856-58860875 | MsG0880045410.01.T01:CDS | 55.0% | |
| !! | ATAGGCATTGAGCCTGGCCA+AGG | - | Chr8:58860857-58860876 | MsG0880045409.01.T02:five_prime_UTR | 55.0% |
| !! | GCGTCCTCGCATGTTGTTTC+TGG | - | Chr8:58861331-58861350 | MsG0880045409.01.T02:five_prime_UTR | 55.0% |
| !! | TCGTATCTGAAGGGTGCCGA+CGG | - | Chr8:58860886-58860905 | MsG0880045409.01.T02:five_prime_UTR | 55.0% |
| GCATTGAGCCTGGCCAAGGT+AGG | - | Chr8:58860853-58860872 | MsG0880045409.01.T02:five_prime_UTR | 60.0% | |
| GGACGCATGTACCGTTTCCC+TGG | + | Chr8:58861345-58861364 | MsG0880045410.01.T01:CDS | 60.0% | |
| TCACTCAGCCTACCTTGGCC+AGG | + | Chr8:58860842-58860861 | MsG0880045410.01.T01:CDS | 60.0% | |
| !! | GGGTGCCGACGGTATTCCAT+AGG | - | Chr8:58860875-58860894 | MsG0880045409.01.T02:five_prime_UTR | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 58860832 | 58861404 | 58860832 | ID=MsG0880045410.01;Name=MsG0880045410.01 |
| Chr8 | mRNA | 58860832 | 58861404 | 58860832 | ID=MsG0880045410.01.T01;Parent=MsG0880045410.01;Name=MsG0880045410.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|190 |
| Chr8 | exon | 58860832 | 58861404 | 58860832 | ID=MsG0880045410.01.T01:exon:22612;Parent=MsG0880045410.01.T01 |
| Chr8 | CDS | 58860832 | 58861404 | 58860832 | ID=MsG0880045410.01.T01:cds;Parent=MsG0880045410.01.T01 |
| Gene Sequence |
| Protein sequence |
