Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045399.01 | MsG0880045405.01 | 0.945568 | 2.390996e-104 | 3.694239e-99 |
| MsG0880045405.01 | MsG0880045407.01 | 0.809905 | 1.501833e-50 | 8.927392e-48 |
| MsG0880045405.01 | MsG0880045408.01 | 0.954006 | 7.791172e-112 | 2.129736e-106 |
| MsG0880045405.01 | MsG0880045410.01 | 0.862902 | 3.645538e-64 | 1.085134e-60 |
| MsG0880045405.01 | MsG0880045422.01 | 0.817609 | 3.018010e-52 | 2.208166e-49 |
| MsG0780040424.01 | MsG0880045405.01 | 0.817508 | 3.179895e-52 | 2.320293e-49 |
| MsG0280006679.01 | MsG0880045405.01 | 0.803119 | 4.058917e-49 | 2.022975e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 52 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTCCTCGCATGTTGTTTC+TGG | 0.119405 | 8:-58827533 | None:intergenic |
| AATTTCACTGAGAACAAAAT+TGG | 0.222803 | 8:-58827275 | None:intergenic |
| ATCTTTGATACGCCGATTAT+CGG | 0.298722 | 8:-58827152 | None:intergenic |
| GCGAACCATCGTCTCCAATC+TGG | 0.357900 | 8:+58827348 | MsG0880045405.01.T01:CDS |
| TGGCCGTTGCAATTTGAGTC+AGG | 0.371578 | 8:-58827513 | None:intergenic |
| TGGTGGTTGGATCGATCATT+TGG | 0.399953 | 8:+58827324 | MsG0880045405.01.T01:CDS |
| CATTGGATGAGGAATCTTCC+AGG | 0.408974 | 8:+58827443 | MsG0880045405.01.T01:CDS |
| TGCTACAATTCGTTGTTGCC+TGG | 0.410074 | 8:-58827461 | None:intergenic |
| TCGACAAAGATCCCGATAAT+CGG | 0.419327 | 8:+58827140 | MsG0880045405.01.T01:CDS |
| GATAATCGGCGTATCAAAGA+TGG | 0.443540 | 8:+58827154 | MsG0880045405.01.T01:CDS |
| TCTTTGATACGCCGATTATC+GGG | 0.444431 | 8:-58827151 | None:intergenic |
| AGATATGCGGTTCCCCAGAT+TGG | 0.453379 | 8:-58827362 | None:intergenic |
| GTATGTTTCAATGATGAAAC+TGG | 0.460739 | 8:-58827594 | None:intergenic |
| CGAACCATCGTCTCCAATCT+GGG | 0.465629 | 8:+58827349 | MsG0880045405.01.T01:CDS |
| TTGGCCAGGCTCAATGCCTA+TGG | 0.471299 | 8:+58827061 | MsG0880045405.01.T01:CDS |
| TACCGTTTCCCTGGTCAACC+TGG | 0.480068 | 8:+58827559 | MsG0880045405.01.T01:CDS |
| TGCAATTTGAGTCAGGGCAG+CGG | 0.490704 | 8:-58827506 | None:intergenic |
| CTCTATCGTTCCGTGGTGGT+TGG | 0.492399 | 8:+58827311 | MsG0880045405.01.T01:CDS |
| AACTGGTGATCGACATATCC+AGG | 0.502872 | 8:-58827577 | None:intergenic |
| GCATGGTAAGGATTTAATTG+AGG | 0.507031 | 8:+58827237 | MsG0880045405.01.T01:CDS |
| TCGACATATCCAGGTTGACC+AGG | 0.507098 | 8:-58827568 | None:intergenic |
| TGTTCGCAATCAAATTGCAT+TGG | 0.510282 | 8:+58827384 | MsG0880045405.01.T01:CDS |
| GATTCAACATATGCATGGTA+AGG | 0.515364 | 8:+58827225 | MsG0880045405.01.T01:CDS |
| GGCAATCACTCAGCCTACCT+TGG | 0.522551 | 8:+58827042 | MsG0880045405.01.T01:CDS |
| ATCCAGGTTGACCAGGGAAA+CGG | 0.524632 | 8:-58827561 | None:intergenic |
| TGCTCAGTTTACATTGGATG+AGG | 0.529316 | 8:+58827432 | MsG0880045405.01.T01:CDS |
| TCACTCAGCCTACCTTGGCC+AGG | 0.531959 | 8:+58827047 | MsG0880045405.01.T01:CDS |
| GGCCGTTGCAATTTGAGTCA+GGG | 0.534236 | 8:-58827512 | None:intergenic |
| GGACGCATGTACCGTTTCCC+TGG | 0.535239 | 8:+58827550 | MsG0880045405.01.T01:CDS |
| TATGTTGAATCAAGTTAAAG+AGG | 0.535573 | 8:-58827213 | None:intergenic |
| TCGTATCTGAAGGGTGCCGA+CGG | 0.543474 | 8:-58827088 | None:intergenic |
| AACTTGATTCAACATATGCA+TGG | 0.547871 | 8:+58827220 | MsG0880045405.01.T01:CDS |
| TATTCCATAGGCATTGAGCC+TGG | 0.563062 | 8:-58827065 | None:intergenic |
| GAAGCATGCTCAGTTTACAT+TGG | 0.564547 | 8:+58827426 | MsG0880045405.01.T01:CDS |
| TGGACTTAGTTAATCAGCAA+AGG | 0.567374 | 8:+58827404 | MsG0880045405.01.T01:CDS |
| GGGTGCCGACGGTATTCCAT+AGG | 0.579023 | 8:-58827077 | None:intergenic |
| ATAGGCATTGAGCCTGGCCA+AGG | 0.589016 | 8:-58827059 | None:intergenic |
| GCATTGAGCCTGGCCAAGGT+AGG | 0.597021 | 8:-58827055 | None:intergenic |
| TGCCCTGACTCAAATTGCAA+CGG | 0.604605 | 8:+58827510 | MsG0880045405.01.T01:CDS |
| ATAATCGGCGTATCAAAGAT+GGG | 0.614224 | 8:+58827155 | MsG0880045405.01.T01:CDS |
| TCACAACGATCACAAAGCAA+TGG | 0.618580 | 8:+58827180 | MsG0880045405.01.T01:CDS |
| GTTGAATCAAGTTAAAGAGG+TGG | 0.634920 | 8:-58827210 | None:intergenic |
| GTTCCCCAGATTGGAGACGA+TGG | 0.654856 | 8:-58827353 | None:intergenic |
| TGCAAAGCTCTATCGTTCCG+TGG | 0.658175 | 8:+58827304 | MsG0880045405.01.T01:CDS |
| CGACATATCCAGGTTGACCA+GGG | 0.659737 | 8:-58827567 | None:intergenic |
| TTTGATTGCGAACAGATATG+CGG | 0.662643 | 8:-58827375 | None:intergenic |
| CAATGCCTATGGAATACCGT+CGG | 0.665714 | 8:+58827072 | MsG0880045405.01.T01:CDS |
| GCAACAACGAATTGTAGCAG+AGG | 0.675734 | 8:+58827465 | MsG0880045405.01.T01:CDS |
| GAACCATCGTCTCCAATCTG+GGG | 0.685733 | 8:+58827350 | MsG0880045405.01.T01:CDS |
| ACGGCCAGAAACAACATGCG+AGG | 0.695230 | 8:+58827529 | MsG0880045405.01.T01:CDS |
| AAAGCTCTATCGTTCCGTGG+TGG | 0.701677 | 8:+58827307 | MsG0880045405.01.T01:CDS |
| AATGATCGATCCAACCACCA+CGG | 0.729748 | 8:-58827321 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTCACTGAGAACAAAAT+TGG | - | Chr8:58827278-58827297 | None:intergenic | 25.0% |
| ! | TATGTTGAATCAAGTTAAAG+AGG | - | Chr8:58827216-58827235 | None:intergenic | 25.0% |
| AACTTGATTCAACATATGCA+TGG | + | Chr8:58827220-58827239 | MsG0880045405.01.T01:CDS | 30.0% | |
| ATAATCGGCGTATCAAAGAT+GGG | + | Chr8:58827155-58827174 | MsG0880045405.01.T01:CDS | 35.0% | |
| GATTCAACATATGCATGGTA+AGG | + | Chr8:58827225-58827244 | MsG0880045405.01.T01:CDS | 35.0% | |
| GCATGGTAAGGATTTAATTG+AGG | + | Chr8:58827237-58827256 | MsG0880045405.01.T01:CDS | 35.0% | |
| GTTGAATCAAGTTAAAGAGG+TGG | - | Chr8:58827213-58827232 | None:intergenic | 35.0% | |
| TGGACTTAGTTAATCAGCAA+AGG | + | Chr8:58827404-58827423 | MsG0880045405.01.T01:CDS | 35.0% | |
| TGTTCGCAATCAAATTGCAT+TGG | + | Chr8:58827384-58827403 | MsG0880045405.01.T01:CDS | 35.0% | |
| TTTGATTGCGAACAGATATG+CGG | - | Chr8:58827378-58827397 | None:intergenic | 35.0% | |
| ! | ATCTTTGATACGCCGATTAT+CGG | - | Chr8:58827155-58827174 | None:intergenic | 35.0% |
| ! | GCATTTTCTTCGTATCTGAA+GGG | - | Chr8:58827100-58827119 | None:intergenic | 35.0% |
| GAAGCATGCTCAGTTTACAT+TGG | + | Chr8:58827426-58827445 | MsG0880045405.01.T01:CDS | 40.0% | |
| GATAATCGGCGTATCAAAGA+TGG | + | Chr8:58827154-58827173 | MsG0880045405.01.T01:CDS | 40.0% | |
| TCACAACGATCACAAAGCAA+TGG | + | Chr8:58827180-58827199 | MsG0880045405.01.T01:CDS | 40.0% | |
| TCGACAAAGATCCCGATAAT+CGG | + | Chr8:58827140-58827159 | MsG0880045405.01.T01:CDS | 40.0% | |
| TGCTCAGTTTACATTGGATG+AGG | + | Chr8:58827432-58827451 | MsG0880045405.01.T01:CDS | 40.0% | |
| ! | CGCATTTTCTTCGTATCTGA+AGG | - | Chr8:58827101-58827120 | None:intergenic | 40.0% |
| ! | TCTTTGATACGCCGATTATC+GGG | - | Chr8:58827154-58827173 | None:intergenic | 40.0% |
| AACTGGTGATCGACATATCC+AGG | - | Chr8:58827580-58827599 | None:intergenic | 45.0% | |
| AATGATCGATCCAACCACCA+CGG | - | Chr8:58827324-58827343 | None:intergenic | 45.0% | |
| CAATGCCTATGGAATACCGT+CGG | + | Chr8:58827072-58827091 | MsG0880045405.01.T01:CDS | 45.0% | |
| CATTGGATGAGGAATCTTCC+AGG | + | Chr8:58827443-58827462 | MsG0880045405.01.T01:CDS | 45.0% | |
| GCAACAACGAATTGTAGCAG+AGG | + | Chr8:58827465-58827484 | MsG0880045405.01.T01:CDS | 45.0% | |
| TGCCCTGACTCAAATTGCAA+CGG | + | Chr8:58827510-58827529 | MsG0880045405.01.T01:CDS | 45.0% | |
| TGCTACAATTCGTTGTTGCC+TGG | - | Chr8:58827464-58827483 | None:intergenic | 45.0% | |
| TGGTGGTTGGATCGATCATT+TGG | + | Chr8:58827324-58827343 | MsG0880045405.01.T01:CDS | 45.0% | |
| !! | TATTCCATAGGCATTGAGCC+TGG | - | Chr8:58827068-58827087 | None:intergenic | 45.0% |
| AAAGCTCTATCGTTCCGTGG+TGG | + | Chr8:58827307-58827326 | MsG0880045405.01.T01:CDS | 50.0% | |
| AGATATGCGGTTCCCCAGAT+TGG | - | Chr8:58827365-58827384 | None:intergenic | 50.0% | |
| CGAACCATCGTCTCCAATCT+GGG | + | Chr8:58827349-58827368 | MsG0880045405.01.T01:CDS | 50.0% | |
| GAACCATCGTCTCCAATCTG+GGG | + | Chr8:58827350-58827369 | MsG0880045405.01.T01:CDS | 50.0% | |
| GGCCGTTGCAATTTGAGTCA+GGG | - | Chr8:58827515-58827534 | None:intergenic | 50.0% | |
| TGCAAAGCTCTATCGTTCCG+TGG | + | Chr8:58827304-58827323 | MsG0880045405.01.T01:CDS | 50.0% | |
| TGCAATTTGAGTCAGGGCAG+CGG | - | Chr8:58827509-58827528 | None:intergenic | 50.0% | |
| TGGCCGTTGCAATTTGAGTC+AGG | - | Chr8:58827516-58827535 | None:intergenic | 50.0% | |
| ! | ATCCAGGTTGACCAGGGAAA+CGG | - | Chr8:58827564-58827583 | None:intergenic | 50.0% |
| ! | CGACATATCCAGGTTGACCA+GGG | - | Chr8:58827570-58827589 | None:intergenic | 50.0% |
| ! | TCGACATATCCAGGTTGACC+AGG | - | Chr8:58827571-58827590 | None:intergenic | 50.0% |
| ACGGCCAGAAACAACATGCG+AGG | + | Chr8:58827529-58827548 | MsG0880045405.01.T01:CDS | 55.0% | |
| CTCTATCGTTCCGTGGTGGT+TGG | + | Chr8:58827311-58827330 | MsG0880045405.01.T01:CDS | 55.0% | |
| GCGAACCATCGTCTCCAATC+TGG | + | Chr8:58827348-58827367 | MsG0880045405.01.T01:CDS | 55.0% | |
| GGCAATCACTCAGCCTACCT+TGG | + | Chr8:58827042-58827061 | MsG0880045405.01.T01:CDS | 55.0% | |
| GTTCCCCAGATTGGAGACGA+TGG | - | Chr8:58827356-58827375 | None:intergenic | 55.0% | |
| TACCGTTTCCCTGGTCAACC+TGG | + | Chr8:58827559-58827578 | MsG0880045405.01.T01:CDS | 55.0% | |
| TTGGCCAGGCTCAATGCCTA+TGG | + | Chr8:58827061-58827080 | MsG0880045405.01.T01:CDS | 55.0% | |
| !! | ATAGGCATTGAGCCTGGCCA+AGG | - | Chr8:58827062-58827081 | None:intergenic | 55.0% |
| !! | GCGTCCTCGCATGTTGTTTC+TGG | - | Chr8:58827536-58827555 | None:intergenic | 55.0% |
| !! | TCGTATCTGAAGGGTGCCGA+CGG | - | Chr8:58827091-58827110 | None:intergenic | 55.0% |
| GCATTGAGCCTGGCCAAGGT+AGG | - | Chr8:58827058-58827077 | None:intergenic | 60.0% | |
| GGACGCATGTACCGTTTCCC+TGG | + | Chr8:58827550-58827569 | MsG0880045405.01.T01:CDS | 60.0% | |
| TCACTCAGCCTACCTTGGCC+AGG | + | Chr8:58827047-58827066 | MsG0880045405.01.T01:CDS | 60.0% | |
| !! | GGGTGCCGACGGTATTCCAT+AGG | - | Chr8:58827080-58827099 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 58827037 | 58827609 | 58827037 | ID=MsG0880045405.01;Name=MsG0880045405.01 |
| Chr8 | mRNA | 58827037 | 58827609 | 58827037 | ID=MsG0880045405.01.T01;Parent=MsG0880045405.01;Name=MsG0880045405.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|190 |
| Chr8 | exon | 58827037 | 58827609 | 58827037 | ID=MsG0880045405.01.T01:exon:22610;Parent=MsG0880045405.01.T01 |
| Chr8 | CDS | 58827037 | 58827609 | 58827037 | ID=MsG0880045405.01.T01:cds;Parent=MsG0880045405.01.T01 |
| Gene Sequence |
| Protein sequence |
