Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045418.01.T01 | XP_024641784.2 | 82.482 | 137 | 24 | 0 | 1 | 137 | 67 | 203 | 1.01E-74 | 231 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045418.01.T01 | A0A2Z6NW35 | 69.853 | 136 | 41 | 0 | 2 | 137 | 259 | 394 | 2.23e-57 | 192 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880045418.01 | MsG0780041116.01 | PPI |
| MsG0880045418.01 | MsG0280009322.01 | PPI |
| MsG0880045418.01 | MsG0780040274.01 | PPI |
| MsG0880045418.01 | MsG0280010449.01 | PPI |
| MsG0880045418.01 | MsG0380013578.01 | PPI |
| MsG0880045418.01 | MsG0780041116.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045418.01.T01 | MTR_7g063280 | 56.863 | 102 | 44 | 0 | 2 | 103 | 74 | 175 | 1.03e-38 | 129 |
| MsG0880045418.01.T01 | MTR_6g016265 | 70.238 | 84 | 25 | 0 | 1 | 84 | 40 | 123 | 2.64e-38 | 126 |
| MsG0880045418.01.T01 | MTR_1g045560 | 55.263 | 114 | 32 | 2 | 6 | 119 | 1 | 95 | 5.77e-34 | 114 |
| MsG0880045418.01.T01 | MTR_2g027020 | 44.118 | 136 | 72 | 1 | 2 | 137 | 30 | 161 | 1.11e-32 | 114 |
| MsG0880045418.01.T01 | MTR_4g036855 | 51.282 | 117 | 29 | 1 | 21 | 137 | 2 | 90 | 1.46e-30 | 105 |
| MsG0880045418.01.T01 | MTR_7g053400 | 49.074 | 108 | 21 | 1 | 6 | 113 | 181 | 254 | 5.89e-28 | 103 |
| MsG0880045418.01.T01 | MTR_0015s0120 | 66.667 | 72 | 24 | 0 | 2 | 73 | 295 | 366 | 3.80e-27 | 103 |
| MsG0880045418.01.T01 | MTR_5g049680 | 64.000 | 75 | 26 | 1 | 2 | 75 | 33 | 107 | 4.25e-27 | 97.4 |
| MsG0880045418.01.T01 | MTR_1g045340 | 51.613 | 93 | 45 | 0 | 1 | 93 | 85 | 177 | 4.34e-27 | 99.8 |
| MsG0880045418.01.T01 | MTR_6g034915 | 43.609 | 133 | 58 | 1 | 4 | 136 | 59 | 174 | 7.51e-27 | 99.0 |
| MsG0880045418.01.T01 | MTR_5g083200 | 52.688 | 93 | 44 | 0 | 1 | 93 | 99 | 191 | 2.02e-26 | 98.2 |
| MsG0880045418.01.T01 | MTR_0002s1220 | 52.688 | 93 | 44 | 0 | 1 | 93 | 99 | 191 | 2.02e-26 | 98.2 |
| MsG0880045418.01.T01 | MTR_3g465840 | 67.647 | 68 | 22 | 0 | 26 | 93 | 1 | 68 | 3.78e-26 | 96.7 |
| MsG0880045418.01.T01 | MTR_3g111390 | 51.613 | 93 | 45 | 0 | 1 | 93 | 99 | 191 | 8.17e-26 | 96.7 |
| MsG0880045418.01.T01 | MTR_1g070820 | 51.613 | 93 | 45 | 0 | 1 | 93 | 99 | 191 | 8.17e-26 | 96.7 |
| MsG0880045418.01.T01 | MTR_1g088650 | 45.631 | 103 | 56 | 0 | 1 | 103 | 87 | 189 | 4.03e-25 | 94.7 |
| MsG0880045418.01.T01 | MTR_6g453270 | 59.211 | 76 | 31 | 0 | 4 | 79 | 304 | 379 | 8.43e-24 | 95.1 |
| MsG0880045418.01.T01 | MTR_1g074090 | 59.375 | 64 | 26 | 0 | 2 | 65 | 83 | 146 | 1.55e-23 | 89.7 |
| MsG0880045418.01.T01 | MTR_5g091440 | 37.121 | 132 | 50 | 1 | 6 | 137 | 1 | 99 | 4.42e-23 | 87.8 |
| MsG0880045418.01.T01 | MTR_3g026270 | 67.273 | 55 | 18 | 0 | 6 | 60 | 1 | 55 | 7.18e-23 | 85.5 |
| MsG0880045418.01.T01 | MTR_5g075610 | 45.455 | 99 | 52 | 1 | 6 | 104 | 1 | 97 | 1.07e-22 | 85.9 |
| MsG0880045418.01.T01 | MTR_3g116210 | 45.055 | 91 | 50 | 0 | 1 | 91 | 98 | 188 | 1.70e-22 | 88.2 |
| MsG0880045418.01.T01 | MTR_2g059470 | 48.193 | 83 | 43 | 0 | 1 | 83 | 1 | 83 | 2.53e-22 | 84.7 |
| MsG0880045418.01.T01 | MTR_7g013520 | 36.029 | 136 | 62 | 1 | 2 | 137 | 72 | 182 | 3.98e-22 | 87.0 |
| MsG0880045418.01.T01 | MTR_1g069475 | 54.430 | 79 | 36 | 0 | 2 | 80 | 166 | 244 | 4.11e-22 | 88.6 |
| MsG0880045418.01.T01 | MTR_7g072030 | 66.667 | 54 | 18 | 0 | 20 | 73 | 69 | 122 | 1.06e-21 | 84.3 |
| MsG0880045418.01.T01 | MTR_0093s0070 | 47.500 | 80 | 42 | 0 | 3 | 82 | 126 | 205 | 4.20e-21 | 84.7 |
| MsG0880045418.01.T01 | MTR_6g016135 | 61.667 | 60 | 23 | 0 | 4 | 63 | 383 | 442 | 4.27e-21 | 87.8 |
| MsG0880045418.01.T01 | MTR_8g031080 | 39.552 | 134 | 55 | 2 | 2 | 135 | 291 | 398 | 4.43e-21 | 87.8 |
| MsG0880045418.01.T01 | MTR_6g089560 | 34.559 | 136 | 67 | 2 | 2 | 137 | 118 | 231 | 8.66e-21 | 84.7 |
| MsG0880045418.01.T01 | MTR_8g088220 | 48.718 | 78 | 38 | 1 | 10 | 87 | 4 | 79 | 3.26e-20 | 79.0 |
| MsG0880045418.01.T01 | MTR_8g098925 | 42.553 | 94 | 54 | 0 | 4 | 97 | 69 | 162 | 6.10e-20 | 80.9 |
| MsG0880045418.01.T01 | MTR_8g028785 | 53.659 | 82 | 38 | 0 | 26 | 107 | 284 | 365 | 1.12e-19 | 83.6 |
| MsG0880045418.01.T01 | MTR_8g059135 | 47.253 | 91 | 48 | 0 | 3 | 93 | 31 | 121 | 1.33e-19 | 79.0 |
| MsG0880045418.01.T01 | MTR_1g034430 | 43.902 | 82 | 46 | 0 | 2 | 83 | 97 | 178 | 2.90e-19 | 79.3 |
| MsG0880045418.01.T01 | MTR_5g085210 | 39.640 | 111 | 51 | 1 | 1 | 111 | 120 | 214 | 6.90e-19 | 79.7 |
| MsG0880045418.01.T01 | MTR_6g013970 | 48.649 | 74 | 38 | 0 | 8 | 81 | 107 | 180 | 7.43e-19 | 79.3 |
| MsG0880045418.01.T01 | MTR_3g010950 | 34.559 | 136 | 59 | 3 | 2 | 137 | 28 | 133 | 2.69e-18 | 75.9 |
| MsG0880045418.01.T01 | MTR_1g081930 | 45.122 | 82 | 45 | 0 | 2 | 83 | 40 | 121 | 4.09e-18 | 75.1 |
| MsG0880045418.01.T01 | MTR_4g008270 | 41.772 | 79 | 46 | 0 | 2 | 80 | 12 | 90 | 1.64e-17 | 72.4 |
| MsG0880045418.01.T01 | MTR_5g037920 | 36.000 | 125 | 74 | 3 | 4 | 125 | 102 | 223 | 2.33e-17 | 75.5 |
| MsG0880045418.01.T01 | MTR_1g050488 | 55.385 | 65 | 29 | 0 | 2 | 66 | 100 | 164 | 2.73e-17 | 73.9 |
| MsG0880045418.01.T01 | MTR_5g083510 | 49.333 | 75 | 38 | 0 | 63 | 137 | 17 | 91 | 9.75e-17 | 70.5 |
| MsG0880045418.01.T01 | MTR_8g031800 | 43.243 | 74 | 42 | 0 | 2 | 75 | 33 | 106 | 1.40e-16 | 70.9 |
| MsG0880045418.01.T01 | MTR_1g052310 | 34.234 | 111 | 73 | 0 | 25 | 135 | 11 | 121 | 1.59e-16 | 70.9 |
| MsG0880045418.01.T01 | MTR_1g062840 | 44.595 | 74 | 41 | 0 | 22 | 95 | 2 | 75 | 3.50e-16 | 68.9 |
| MsG0880045418.01.T01 | MTR_5g034450 | 50.000 | 62 | 31 | 0 | 2 | 63 | 51 | 112 | 4.84e-16 | 69.3 |
| MsG0880045418.01.T01 | MTR_7g006380 | 36.207 | 116 | 47 | 1 | 2 | 117 | 68 | 156 | 7.79e-16 | 70.5 |
| MsG0880045418.01.T01 | MTR_5g031620 | 43.373 | 83 | 47 | 0 | 55 | 137 | 139 | 221 | 3.64e-15 | 69.7 |
| MsG0880045418.01.T01 | MTR_8g036780 | 50.000 | 68 | 34 | 0 | 2 | 69 | 36 | 103 | 6.12e-15 | 66.6 |
| MsG0880045418.01.T01 | MTR_1g074240 | 46.154 | 65 | 35 | 0 | 1 | 65 | 1 | 65 | 7.41e-15 | 65.1 |
| MsG0880045418.01.T01 | MTR_8g099580 | 48.684 | 76 | 39 | 0 | 4 | 79 | 210 | 285 | 7.58e-15 | 69.7 |
| MsG0880045418.01.T01 | MTR_6g007540 | 57.407 | 54 | 23 | 0 | 73 | 126 | 24 | 77 | 3.10e-14 | 65.5 |
| MsG0880045418.01.T01 | MTR_3g090920 | 31.452 | 124 | 69 | 2 | 14 | 137 | 1 | 108 | 4.04e-14 | 64.3 |
| MsG0880045418.01.T01 | MTR_4g029360 | 39.394 | 99 | 60 | 0 | 2 | 100 | 129 | 227 | 5.62e-14 | 66.6 |
| MsG0880045418.01.T01 | MTR_1g057740 | 43.750 | 80 | 45 | 0 | 57 | 136 | 60 | 139 | 9.80e-14 | 64.3 |
| MsG0880045418.01.T01 | MTR_2g088140 | 46.970 | 66 | 35 | 0 | 61 | 126 | 49 | 114 | 1.50e-13 | 63.2 |
| MsG0880045418.01.T01 | MTR_1g017820 | 52.727 | 55 | 26 | 0 | 11 | 65 | 11 | 65 | 3.79e-13 | 60.8 |
| MsG0880045418.01.T01 | MTR_8g079540 | 48.611 | 72 | 37 | 0 | 2 | 73 | 50 | 121 | 3.79e-13 | 62.0 |
| MsG0880045418.01.T01 | MTR_4g098670 | 41.463 | 82 | 46 | 2 | 55 | 135 | 16 | 96 | 5.72e-13 | 60.8 |
| MsG0880045418.01.T01 | MTR_5g069700 | 42.857 | 63 | 36 | 0 | 21 | 83 | 2 | 64 | 5.88e-13 | 60.5 |
| MsG0880045418.01.T01 | MTR_5g014420 | 65.116 | 43 | 15 | 0 | 37 | 79 | 72 | 114 | 6.11e-13 | 61.6 |
| MsG0880045418.01.T01 | MTR_4g064590 | 33.333 | 117 | 72 | 2 | 23 | 137 | 49 | 161 | 6.29e-13 | 62.4 |
| MsG0880045418.01.T01 | MTR_1g017840 | 35.000 | 80 | 52 | 0 | 4 | 83 | 82 | 161 | 7.07e-13 | 62.4 |
| MsG0880045418.01.T01 | MTR_5g036320 | 50.000 | 62 | 31 | 0 | 2 | 63 | 81 | 142 | 1.11e-12 | 61.6 |
| MsG0880045418.01.T01 | MTR_8g038340 | 50.000 | 56 | 28 | 0 | 2 | 57 | 191 | 246 | 2.82e-12 | 62.0 |
| MsG0880045418.01.T01 | MTR_3g071490 | 53.846 | 52 | 24 | 0 | 86 | 137 | 8 | 59 | 1.07e-11 | 56.6 |
| MsG0880045418.01.T01 | MTR_4g073820 | 53.704 | 54 | 23 | 1 | 6 | 59 | 1 | 52 | 1.95e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTGATGCTACAGAATT+TGG | 0.041994 | 8:+58934494 | MsG0880045418.01.T01:CDS |
| ATTCTGTAGCATCACAATTT+TGG | 0.089929 | 8:-58934490 | None:intergenic |
| TATCAGCTAGTTTCCAAATT+TGG | 0.105567 | 8:+58934633 | MsG0880045418.01.T01:CDS |
| CCAATATGTACCACACAAAT+TGG | 0.171084 | 8:-58934362 | None:intergenic |
| CTGGTATTTCTACCCAAATT+TGG | 0.196784 | 8:-58934646 | None:intergenic |
| ATTCAAATATGGTTGGAATA+TGG | 0.313586 | 8:+58934282 | None:intergenic |
| ATCAGCTAGTTTCCAAATTT+GGG | 0.314793 | 8:+58934634 | MsG0880045418.01.T01:CDS |
| TGAGCTTAATCTAGGACCTT+TGG | 0.338357 | 8:+58934424 | MsG0880045418.01.T01:CDS |
| GACTCTTATCTGCATTGGAT+TGG | 0.339537 | 8:+58934396 | MsG0880045418.01.T01:CDS |
| CCAATTTGTGTGGTACATAT+TGG | 0.354405 | 8:+58934362 | MsG0880045418.01.T01:CDS |
| ACTCTTATCTGCATTGGATT+GGG | 0.362620 | 8:+58934397 | MsG0880045418.01.T01:CDS |
| ATTGTGATGCTACAGAATTT+GGG | 0.434380 | 8:+58934495 | MsG0880045418.01.T01:CDS |
| TTTGAGAATTCAAATATGGT+TGG | 0.477116 | 8:+58934275 | None:intergenic |
| CAATATGTACCACACAAATT+GGG | 0.482215 | 8:-58934361 | None:intergenic |
| TGGGTTCATGAGCTTAATCT+AGG | 0.486420 | 8:+58934416 | MsG0880045418.01.T01:CDS |
| TCTAGGACTCTTATCTGCAT+TGG | 0.489881 | 8:+58934391 | MsG0880045418.01.T01:CDS |
| ATGTGTATCAGAGATGAAAA+TGG | 0.503178 | 8:+58934305 | MsG0880045418.01.T01:CDS |
| AATATGTGTTCAATACAATC+TGG | 0.503895 | 8:-58934665 | None:intergenic |
| GTACATATTGGAGAAGCTCT+AGG | 0.562489 | 8:+58934374 | MsG0880045418.01.T01:CDS |
| TGAACTAGACGCAAAGAGAG+TGG | 0.603826 | 8:+58934451 | MsG0880045418.01.T01:CDS |
| GCGTCTAGTTCAAAATCCAA+AGG | 0.607631 | 8:-58934440 | None:intergenic |
| TTGGAAACTAGCTGATAACA+AGG | 0.609482 | 8:-58934627 | None:intergenic |
| ATATTTCAGCCCAATTTGTG+TGG | 0.619691 | 8:+58934352 | MsG0880045418.01.T01:CDS |
| TGTGCAGAGACAAGCAAACG+AGG | 0.671801 | 8:+58934580 | MsG0880045418.01.T01:CDS |
| ACTAGACGCAAAGAGAGTGG+TGG | 0.680932 | 8:+58934454 | MsG0880045418.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATGTGTTCAATACAATC+TGG | - | Chr8:58934668-58934687 | None:intergenic | 25.0% |
| AATTGTGATGCTACAGAATT+TGG | + | Chr8:58934494-58934513 | MsG0880045418.01.T01:CDS | 30.0% | |
| ATGTGTATCAGAGATGAAAA+TGG | + | Chr8:58934305-58934324 | MsG0880045418.01.T01:CDS | 30.0% | |
| ATTGTGATGCTACAGAATTT+GGG | + | Chr8:58934495-58934514 | MsG0880045418.01.T01:CDS | 30.0% | |
| CAATATGTACCACACAAATT+GGG | - | Chr8:58934364-58934383 | None:intergenic | 30.0% | |
| ! | ATCAGCTAGTTTCCAAATTT+GGG | + | Chr8:58934634-58934653 | MsG0880045418.01.T01:CDS | 30.0% |
| ! | ATTCTGTAGCATCACAATTT+TGG | - | Chr8:58934493-58934512 | None:intergenic | 30.0% |
| ! | TATCAGCTAGTTTCCAAATT+TGG | + | Chr8:58934633-58934652 | MsG0880045418.01.T01:CDS | 30.0% |
| ! | TGAAAATGGTATTTTCGCTT+TGG | + | Chr8:58934319-58934338 | MsG0880045418.01.T01:CDS | 30.0% |
| ! | TTCTGTAGCATCACAATTTT+GGG | - | Chr8:58934492-58934511 | None:intergenic | 30.0% |
| ACTCTTATCTGCATTGGATT+GGG | + | Chr8:58934397-58934416 | MsG0880045418.01.T01:CDS | 35.0% | |
| ATATTTCAGCCCAATTTGTG+TGG | + | Chr8:58934352-58934371 | MsG0880045418.01.T01:CDS | 35.0% | |
| CCAATATGTACCACACAAAT+TGG | - | Chr8:58934365-58934384 | None:intergenic | 35.0% | |
| CCAATTTGTGTGGTACATAT+TGG | + | Chr8:58934362-58934381 | MsG0880045418.01.T01:CDS | 35.0% | |
| TTGGAAACTAGCTGATAACA+AGG | - | Chr8:58934630-58934649 | None:intergenic | 35.0% | |
| ! | CTGGTATTTCTACCCAAATT+TGG | - | Chr8:58934649-58934668 | None:intergenic | 35.0% |
| GACTCTTATCTGCATTGGAT+TGG | + | Chr8:58934396-58934415 | MsG0880045418.01.T01:CDS | 40.0% | |
| GTACATATTGGAGAAGCTCT+AGG | + | Chr8:58934374-58934393 | MsG0880045418.01.T01:CDS | 40.0% | |
| TGGGTTCATGAGCTTAATCT+AGG | + | Chr8:58934416-58934435 | MsG0880045418.01.T01:CDS | 40.0% | |
| ! | GCGTCTAGTTCAAAATCCAA+AGG | - | Chr8:58934443-58934462 | None:intergenic | 40.0% |
| ! | TCTAGGACTCTTATCTGCAT+TGG | + | Chr8:58934391-58934410 | MsG0880045418.01.T01:CDS | 40.0% |
| ! | TGAGCTTAATCTAGGACCTT+TGG | + | Chr8:58934424-58934443 | MsG0880045418.01.T01:CDS | 40.0% |
| TGAACTAGACGCAAAGAGAG+TGG | + | Chr8:58934451-58934470 | MsG0880045418.01.T01:CDS | 45.0% | |
| ACTAGACGCAAAGAGAGTGG+TGG | + | Chr8:58934454-58934473 | MsG0880045418.01.T01:CDS | 50.0% | |
| TGTGCAGAGACAAGCAAACG+AGG | + | Chr8:58934580-58934599 | MsG0880045418.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 58934290 | 58934703 | 58934290 | ID=MsG0880045418.01;Name=MsG0880045418.01 |
| Chr8 | mRNA | 58934290 | 58934703 | 58934290 | ID=MsG0880045418.01.T01;Parent=MsG0880045418.01;Name=MsG0880045418.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr8 | exon | 58934290 | 58934703 | 58934290 | ID=MsG0880045418.01.T01:exon:12094;Parent=MsG0880045418.01.T01 |
| Chr8 | CDS | 58934290 | 58934703 | 58934290 | ID=MsG0880045418.01.T01:cds;Parent=MsG0880045418.01.T01 |
| Gene Sequence |
| Protein sequence |
