Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045800.01.T01 | XP_013446470.1 | 90.368 | 353 | 23 | 5 | 1 | 352 | 1 | 343 | 0 | 560 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045800.01.T01 | Q84TE9 | 58.416 | 101 | 35 | 3 | 63 | 160 | 42 | 138 | 4.46E-32 | 124 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045800.01.T01 | A0A072TSV2 | 90.368 | 353 | 23 | 5 | 1 | 352 | 1 | 343 | 0.0 | 560 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880045800.01.T01 | TF | C2C2-Dof |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048701.01 | MsG0880045800.01 | 0.827123 | 1.861742e-54 | 1.780793e-51 |
| MsG0480019588.01 | MsG0880045800.01 | 0.819160 | 1.344003e-52 | 1.026238e-49 |
| MsG0480019699.01 | MsG0880045800.01 | 0.806599 | 7.606803e-50 | 4.145691e-47 |
| MsG0380017667.01 | MsG0880045800.01 | 0.808748 | 2.659598e-50 | 1.532992e-47 |
| MsG0580027049.01 | MsG0880045800.01 | 0.824386 | 8.300482e-54 | 7.340896e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045800.01.T01 | MTR_8g479350 | 90.368 | 353 | 23 | 5 | 1 | 352 | 1 | 343 | 0.0 | 560 |
| MsG0880045800.01.T01 | MTR_2g096740 | 76.190 | 63 | 15 | 0 | 73 | 135 | 77 | 139 | 5.86e-33 | 124 |
| MsG0880045800.01.T01 | MTR_4g063780 | 68.354 | 79 | 16 | 1 | 70 | 139 | 72 | 150 | 5.99e-33 | 125 |
| MsG0880045800.01.T01 | MTR_2g096740 | 76.190 | 63 | 15 | 0 | 73 | 135 | 62 | 124 | 6.08e-33 | 124 |
| MsG0880045800.01.T01 | MTR_7g024670 | 76.562 | 64 | 15 | 0 | 74 | 137 | 78 | 141 | 1.14e-31 | 122 |
| MsG0880045800.01.T01 | MTR_8g068210 | 55.556 | 90 | 40 | 0 | 60 | 149 | 19 | 108 | 6.22e-31 | 120 |
| MsG0880045800.01.T01 | MTR_5g031440 | 92.157 | 51 | 4 | 0 | 73 | 123 | 43 | 93 | 8.67e-31 | 119 |
| MsG0880045800.01.T01 | MTR_4g088580 | 79.661 | 59 | 12 | 0 | 74 | 132 | 56 | 114 | 1.05e-30 | 120 |
| MsG0880045800.01.T01 | MTR_3g090430 | 62.025 | 79 | 26 | 1 | 52 | 126 | 26 | 104 | 2.61e-30 | 118 |
| MsG0880045800.01.T01 | MTR_7g059400 | 78.947 | 57 | 12 | 0 | 70 | 126 | 73 | 129 | 3.81e-30 | 118 |
| MsG0880045800.01.T01 | MTR_8g079060 | 57.143 | 84 | 36 | 0 | 65 | 148 | 21 | 104 | 7.95e-30 | 114 |
| MsG0880045800.01.T01 | MTR_8g027295 | 63.158 | 76 | 28 | 0 | 70 | 145 | 21 | 96 | 8.31e-30 | 115 |
| MsG0880045800.01.T01 | MTR_3g091820 | 62.338 | 77 | 29 | 0 | 50 | 126 | 20 | 96 | 1.40e-29 | 115 |
| MsG0880045800.01.T01 | MTR_4g461080 | 84.906 | 53 | 8 | 0 | 73 | 125 | 29 | 81 | 1.93e-29 | 114 |
| MsG0880045800.01.T01 | MTR_4g022370 | 81.481 | 54 | 10 | 0 | 73 | 126 | 82 | 135 | 2.14e-29 | 116 |
| MsG0880045800.01.T01 | MTR_4g109980 | 86.275 | 51 | 7 | 0 | 73 | 123 | 40 | 90 | 3.54e-29 | 115 |
| MsG0880045800.01.T01 | MTR_8g015840 | 80.000 | 55 | 11 | 0 | 70 | 124 | 17 | 71 | 4.19e-29 | 112 |
| MsG0880045800.01.T01 | MTR_2g093220 | 85.185 | 54 | 8 | 0 | 73 | 126 | 40 | 93 | 6.56e-29 | 113 |
| MsG0880045800.01.T01 | MTR_1g056810 | 81.034 | 58 | 11 | 0 | 69 | 126 | 8 | 65 | 2.44e-28 | 112 |
| MsG0880045800.01.T01 | MTR_2g014060 | 83.636 | 55 | 8 | 1 | 70 | 124 | 23 | 76 | 4.02e-28 | 112 |
| MsG0880045800.01.T01 | MTR_4g089095 | 78.947 | 57 | 11 | 1 | 72 | 128 | 49 | 104 | 1.28e-27 | 110 |
| MsG0880045800.01.T01 | MTR_3g077750 | 78.571 | 56 | 9 | 1 | 71 | 123 | 56 | 111 | 1.29e-27 | 111 |
| MsG0880045800.01.T01 | MTR_4g089095 | 78.947 | 57 | 11 | 1 | 72 | 128 | 15 | 70 | 1.32e-27 | 109 |
| MsG0880045800.01.T01 | MTR_2g059540 | 85.714 | 49 | 7 | 0 | 73 | 121 | 31 | 79 | 2.79e-27 | 107 |
| MsG0880045800.01.T01 | MTR_1g077600 | 72.414 | 58 | 16 | 0 | 68 | 125 | 38 | 95 | 9.40e-27 | 107 |
| MsG0880045800.01.T01 | MTR_1g077600 | 72.414 | 58 | 16 | 0 | 68 | 125 | 38 | 95 | 9.43e-27 | 107 |
| MsG0880045800.01.T01 | MTR_2g014170 | 83.673 | 49 | 8 | 0 | 73 | 121 | 45 | 93 | 1.06e-26 | 108 |
| MsG0880045800.01.T01 | MTR_2g016030 | 50.515 | 97 | 42 | 2 | 75 | 171 | 49 | 139 | 2.41e-26 | 103 |
| MsG0880045800.01.T01 | MTR_2g013370 | 73.214 | 56 | 14 | 1 | 73 | 128 | 46 | 100 | 1.43e-25 | 104 |
| MsG0880045800.01.T01 | MTR_4g082060 | 50.000 | 98 | 43 | 2 | 40 | 131 | 83 | 180 | 3.41e-25 | 106 |
| MsG0880045800.01.T01 | MTR_6g012450 | 75.926 | 54 | 13 | 0 | 73 | 126 | 136 | 189 | 3.86e-25 | 106 |
| MsG0880045800.01.T01 | MTR_7g010950 | 43.966 | 116 | 47 | 2 | 30 | 127 | 74 | 189 | 6.63e-25 | 105 |
| MsG0880045800.01.T01 | MTR_6g027460 | 35.955 | 178 | 95 | 4 | 41 | 204 | 60 | 232 | 6.97e-25 | 103 |
| MsG0880045800.01.T01 | MTR_7g086780 | 57.895 | 76 | 32 | 0 | 73 | 148 | 99 | 174 | 7.16e-25 | 104 |
| MsG0880045800.01.T01 | MTR_5g041420 | 52.577 | 97 | 40 | 2 | 44 | 135 | 105 | 200 | 1.32e-24 | 102 |
| MsG0880045800.01.T01 | MTR_3g435480 | 72.222 | 54 | 15 | 0 | 73 | 126 | 136 | 189 | 1.85e-24 | 103 |
| MsG0880045800.01.T01 | MTR_8g044220 | 56.164 | 73 | 32 | 0 | 73 | 145 | 118 | 190 | 2.60e-24 | 103 |
| MsG0880045800.01.T01 | MTR_6g027450 | 47.826 | 92 | 44 | 2 | 41 | 128 | 53 | 144 | 3.91e-24 | 101 |
| MsG0880045800.01.T01 | MTR_5g041530 | 67.188 | 64 | 20 | 1 | 75 | 138 | 142 | 204 | 1.32e-23 | 100 |
| MsG0880045800.01.T01 | MTR_5g041380 | 47.872 | 94 | 47 | 1 | 44 | 135 | 96 | 189 | 1.46e-22 | 97.8 |
| MsG0880045800.01.T01 | MTR_5g041400 | 48.936 | 94 | 46 | 1 | 44 | 135 | 88 | 181 | 1.49e-22 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045800.01.T01 | AT5G60200 | 58.416 | 101 | 35 | 3 | 63 | 160 | 42 | 138 | 4.54e-33 | 124 |
| MsG0880045800.01.T01 | AT5G65590 | 55.102 | 98 | 44 | 0 | 40 | 137 | 8 | 105 | 3.41e-32 | 123 |
| MsG0880045800.01.T01 | AT1G07640 | 90.566 | 53 | 5 | 0 | 73 | 125 | 21 | 73 | 6.58e-32 | 121 |
| MsG0880045800.01.T01 | AT1G07640 | 90.566 | 53 | 5 | 0 | 73 | 125 | 77 | 129 | 9.81e-32 | 122 |
| MsG0880045800.01.T01 | AT1G51700 | 63.750 | 80 | 23 | 1 | 51 | 124 | 5 | 84 | 1.26e-31 | 118 |
| MsG0880045800.01.T01 | AT1G07640 | 90.566 | 53 | 5 | 0 | 73 | 125 | 85 | 137 | 1.28e-31 | 122 |
| MsG0880045800.01.T01 | AT2G28810 | 86.538 | 52 | 7 | 0 | 73 | 124 | 77 | 128 | 3.93e-31 | 120 |
| MsG0880045800.01.T01 | AT2G28810 | 86.538 | 52 | 7 | 0 | 73 | 124 | 94 | 145 | 5.80e-31 | 120 |
| MsG0880045800.01.T01 | AT5G60850 | 72.222 | 72 | 15 | 1 | 73 | 139 | 51 | 122 | 7.30e-31 | 119 |
| MsG0880045800.01.T01 | AT2G37590 | 78.947 | 57 | 12 | 0 | 70 | 126 | 86 | 142 | 1.20e-30 | 119 |
| MsG0880045800.01.T01 | AT3G21270 | 88.679 | 53 | 6 | 0 | 73 | 125 | 29 | 81 | 1.24e-30 | 115 |
| MsG0880045800.01.T01 | AT3G45610 | 69.697 | 66 | 20 | 0 | 73 | 138 | 40 | 105 | 7.97e-30 | 115 |
| MsG0880045800.01.T01 | AT5G02460 | 83.333 | 54 | 9 | 0 | 73 | 126 | 95 | 148 | 1.49e-29 | 117 |
| MsG0880045800.01.T01 | AT3G55370 | 50.467 | 107 | 48 | 2 | 33 | 139 | 83 | 184 | 1.91e-29 | 116 |
| MsG0880045800.01.T01 | AT3G55370 | 73.134 | 67 | 15 | 1 | 73 | 139 | 76 | 139 | 2.04e-29 | 115 |
| MsG0880045800.01.T01 | AT3G55370 | 50.467 | 107 | 48 | 2 | 33 | 139 | 38 | 139 | 2.05e-29 | 116 |
| MsG0880045800.01.T01 | AT4G38000 | 67.105 | 76 | 24 | 1 | 52 | 126 | 20 | 95 | 2.52e-29 | 113 |
| MsG0880045800.01.T01 | AT1G28310 | 53.125 | 96 | 44 | 1 | 34 | 128 | 11 | 106 | 1.15e-28 | 114 |
| MsG0880045800.01.T01 | AT3G50410 | 73.016 | 63 | 17 | 0 | 63 | 125 | 20 | 82 | 1.35e-28 | 112 |
| MsG0880045800.01.T01 | AT1G28310 | 78.571 | 56 | 12 | 0 | 73 | 128 | 27 | 82 | 1.74e-28 | 113 |
| MsG0880045800.01.T01 | AT3G61850 | 56.383 | 94 | 40 | 1 | 34 | 126 | 22 | 115 | 2.08e-28 | 112 |
| MsG0880045800.01.T01 | AT3G61850 | 56.383 | 94 | 40 | 1 | 34 | 126 | 22 | 115 | 2.08e-28 | 112 |
| MsG0880045800.01.T01 | AT3G61850 | 56.383 | 94 | 40 | 1 | 34 | 126 | 34 | 127 | 2.20e-28 | 112 |
| MsG0880045800.01.T01 | AT3G61850 | 56.383 | 94 | 40 | 1 | 34 | 126 | 22 | 115 | 3.02e-28 | 112 |
| MsG0880045800.01.T01 | AT3G61850 | 56.383 | 94 | 40 | 1 | 34 | 126 | 34 | 127 | 3.22e-28 | 112 |
| MsG0880045800.01.T01 | AT3G52440 | 84.615 | 52 | 8 | 0 | 75 | 126 | 27 | 78 | 3.37e-28 | 110 |
| MsG0880045800.01.T01 | AT2G28510 | 82.692 | 52 | 9 | 0 | 75 | 126 | 50 | 101 | 3.58e-28 | 111 |
| MsG0880045800.01.T01 | AT3G52440 | 84.615 | 52 | 8 | 0 | 75 | 126 | 46 | 97 | 4.25e-28 | 110 |
| MsG0880045800.01.T01 | AT5G66940 | 60.274 | 73 | 29 | 0 | 67 | 139 | 26 | 98 | 4.67e-28 | 109 |
| MsG0880045800.01.T01 | AT1G21340 | 55.056 | 89 | 39 | 1 | 75 | 162 | 40 | 128 | 1.34e-27 | 109 |
| MsG0880045800.01.T01 | AT4G00940 | 57.471 | 87 | 37 | 0 | 63 | 149 | 58 | 144 | 2.31e-27 | 109 |
| MsG0880045800.01.T01 | AT4G00940 | 57.471 | 87 | 37 | 0 | 63 | 149 | 58 | 144 | 2.31e-27 | 109 |
| MsG0880045800.01.T01 | AT4G00940 | 57.471 | 87 | 37 | 0 | 63 | 149 | 58 | 144 | 2.31e-27 | 109 |
| MsG0880045800.01.T01 | AT4G21050 | 65.625 | 64 | 22 | 0 | 71 | 134 | 22 | 85 | 5.63e-27 | 106 |
| MsG0880045800.01.T01 | AT4G24060 | 70.968 | 62 | 18 | 0 | 65 | 126 | 45 | 106 | 9.71e-27 | 108 |
| MsG0880045800.01.T01 | AT5G62940 | 80.000 | 50 | 10 | 0 | 74 | 123 | 74 | 123 | 4.27e-26 | 107 |
| MsG0880045800.01.T01 | AT1G29160 | 58.667 | 75 | 31 | 0 | 52 | 126 | 41 | 115 | 3.29e-25 | 100 |
| MsG0880045800.01.T01 | AT5G39660 | 51.402 | 107 | 35 | 1 | 38 | 127 | 86 | 192 | 3.32e-25 | 106 |
| MsG0880045800.01.T01 | AT5G39660 | 51.402 | 107 | 35 | 1 | 38 | 127 | 86 | 192 | 3.32e-25 | 106 |
| MsG0880045800.01.T01 | AT5G39660 | 51.402 | 107 | 35 | 1 | 38 | 127 | 86 | 192 | 3.32e-25 | 106 |
| MsG0880045800.01.T01 | AT2G46590 | 52.809 | 89 | 40 | 1 | 34 | 120 | 27 | 115 | 6.12e-25 | 104 |
| MsG0880045800.01.T01 | AT2G46590 | 52.809 | 89 | 40 | 1 | 34 | 120 | 39 | 127 | 7.18e-25 | 104 |
| MsG0880045800.01.T01 | AT3G47500 | 74.545 | 55 | 14 | 0 | 73 | 127 | 110 | 164 | 7.55e-25 | 105 |
| MsG0880045800.01.T01 | AT2G34140 | 55.263 | 76 | 34 | 0 | 51 | 126 | 36 | 111 | 8.90e-25 | 99.4 |
| MsG0880045800.01.T01 | AT1G69570 | 55.844 | 77 | 34 | 0 | 63 | 139 | 122 | 198 | 1.74e-24 | 103 |
| MsG0880045800.01.T01 | AT1G64620 | 71.930 | 57 | 16 | 0 | 65 | 121 | 41 | 97 | 1.79e-24 | 102 |
| MsG0880045800.01.T01 | AT1G26790 | 45.794 | 107 | 51 | 3 | 57 | 160 | 92 | 194 | 3.67e-24 | 102 |
| MsG0880045800.01.T01 | AT5G62430 | 74.074 | 54 | 14 | 0 | 73 | 126 | 54 | 107 | 4.92e-24 | 100 |
| MsG0880045800.01.T01 | AT1G47655 | 61.972 | 71 | 23 | 2 | 54 | 121 | 8 | 77 | 1.33e-23 | 97.4 |
| MsG0880045800.01.T01 | AT4G21040 | 73.585 | 53 | 14 | 0 | 75 | 127 | 27 | 79 | 4.80e-23 | 96.3 |
| MsG0880045800.01.T01 | AT4G21080 | 61.538 | 65 | 25 | 0 | 75 | 139 | 27 | 91 | 2.50e-22 | 94.7 |
| MsG0880045800.01.T01 | AT4G21030 | 56.000 | 50 | 22 | 0 | 75 | 124 | 23 | 72 | 1.90e-13 | 68.6 |
Find 102 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATGATTGTTTCTCCAAAA+TGG | 0.139233 | 8:-64656318 | None:intergenic |
| TTCCTCTATATCCAACATTT+TGG | 0.175939 | 8:+64656542 | MsG0880045800.01.T01:CDS |
| TCAGTTACTGGTGGTGAATT+AGG | 0.188537 | 8:+64656573 | MsG0880045800.01.T01:CDS |
| TCCTCCAACTCCTCCATTAA+TGG | 0.195655 | 8:-64655683 | None:intergenic |
| GTTATACTTATTACTGATAT+TGG | 0.212000 | 8:-64656662 | None:intergenic |
| CATGATTAGGGTTAGGGTTT+TGG | 0.228883 | 8:-64656185 | None:intergenic |
| CCTTCTTCCTTAACATGATT+AGG | 0.237816 | 8:-64656198 | None:intergenic |
| CACCATTACCAAAGAGTTCT+TGG | 0.238413 | 8:-64655621 | None:intergenic |
| GTGGTGGTGGTTGTGGAAGA+TGG | 0.243950 | 8:-64655745 | None:intergenic |
| TTAGTTTCTGGTACAGTTCT+TGG | 0.259576 | 8:-64656419 | None:intergenic |
| CTTCTTCCTTAACATGATTA+GGG | 0.273935 | 8:-64656197 | None:intergenic |
| AGAAACTACTCTTCCATTAA+TGG | 0.274062 | 8:+64655670 | MsG0880045800.01.T01:CDS |
| ACAGTTTCTTCAGAGTTTAA+TGG | 0.275960 | 8:+64656045 | MsG0880045800.01.T01:CDS |
| GCAAGACATGCCGTCGTTAC+TGG | 0.280270 | 8:+64655914 | MsG0880045800.01.T01:CDS |
| TTGGAAGATCAGACCAAGTT+AGG | 0.282710 | 8:-64656617 | None:intergenic |
| TTGGAGACAACTTCAGTTAC+TGG | 0.287644 | 8:+64656561 | MsG0880045800.01.T01:CDS |
| TTGTTGATGACCTTAGTTTC+TGG | 0.307492 | 8:-64656431 | None:intergenic |
| GAAGAAGGCAATAACTTCAT+AGG | 0.324773 | 8:+64656213 | MsG0880045800.01.T01:CDS |
| TGGTGCAGTGGTGGTGGTGG+TGG | 0.332078 | 8:-64655761 | None:intergenic |
| TTATACTTATTACTGATATT+GGG | 0.334867 | 8:-64656661 | None:intergenic |
| CAAAATGGTGGATTTGTACT+TGG | 0.338045 | 8:+64656360 | MsG0880045800.01.T01:CDS |
| CAGTTACTGGTGGTGAATTA+GGG | 0.346171 | 8:+64656574 | MsG0880045800.01.T01:CDS |
| CCTTAACATGATTAGGGTTA+GGG | 0.353211 | 8:-64656191 | None:intergenic |
| CAAACTCAACAATTACAAAA+TGG | 0.354663 | 8:+64656345 | MsG0880045800.01.T01:CDS |
| GACCAAGTTAGGGTTGGAAT+TGG | 0.358551 | 8:-64656606 | None:intergenic |
| TCCTTAACATGATTAGGGTT+AGG | 0.359817 | 8:-64656192 | None:intergenic |
| CCAACACCAACAGTGTTACT+AGG | 0.367483 | 8:+64656501 | MsG0880045800.01.T01:CDS |
| CATGATTGTTTCTCCAAAAT+GGG | 0.372690 | 8:-64656317 | None:intergenic |
| CAGAAAACCAACCACATCTA+AGG | 0.374661 | 8:+64655815 | MsG0880045800.01.T01:CDS |
| TGGTGGTGGTTGTGGAAGAT+GGG | 0.383636 | 8:-64655744 | None:intergenic |
| TTTCCGACAGCCACCTCCTA+TGG | 0.383757 | 8:-64655965 | None:intergenic |
| GACGGCATGTCTTGCAGAAA+TGG | 0.400957 | 8:-64655906 | None:intergenic |
| CACCACAAAGTCAAATCCTC+TGG | 0.406936 | 8:+64656112 | MsG0880045800.01.T01:CDS |
| GAAGAAGGAGGTTGTGTTGT+TGG | 0.410788 | 8:-64655718 | None:intergenic |
| CCCTAACCCTAATCATGTTA+AGG | 0.421766 | 8:+64656191 | MsG0880045800.01.T01:CDS |
| CCCCAGAGGATTTGACTTTG+TGG | 0.425921 | 8:-64656114 | None:intergenic |
| TGCGGTGGTGGTTGTGGTGA+CGG | 0.433800 | 8:-64655788 | None:intergenic |
| CTCCAAAATGTTGGATATAG+AGG | 0.436023 | 8:-64656544 | None:intergenic |
| CAGCCGTTGATTTCGTCAAA+TGG | 0.436916 | 8:+64656264 | MsG0880045800.01.T01:CDS |
| TCCATTAATGGAGGAGTTGG+AGG | 0.440892 | 8:+64655682 | MsG0880045800.01.T01:CDS |
| CCTAATCATGTTAAGGAAGA+AGG | 0.447706 | 8:+64656198 | MsG0880045800.01.T01:CDS |
| GTGGTTGTGGTGACGGTGGT+TGG | 0.448495 | 8:-64655781 | None:intergenic |
| GTGGAAGATGGGCGTGAAGA+AGG | 0.452402 | 8:-64655733 | None:intergenic |
| GGACAAAAGGAGGTGCTTTG+AGG | 0.473653 | 8:+64655935 | MsG0880045800.01.T01:CDS |
| ATCCAATTCCAACCCTAACT+TGG | 0.473948 | 8:+64656604 | MsG0880045800.01.T01:CDS |
| GGTGGTTGGTGCAGTGGTGG+TGG | 0.475125 | 8:-64655767 | None:intergenic |
| AAGATGATCCAAGAACTCTT+TGG | 0.477513 | 8:+64655613 | None:intergenic |
| TGCCGTCGTTACTGGACAAA+AGG | 0.477938 | 8:+64655922 | MsG0880045800.01.T01:CDS |
| AAAATGGTGGATTTGTACTT+GGG | 0.478306 | 8:+64656361 | MsG0880045800.01.T01:CDS |
| ATTTCGTCAAATGGTTATGA+TGG | 0.478918 | 8:+64656273 | MsG0880045800.01.T01:CDS |
| TGATGATGCGGTGGTGGTTG+TGG | 0.481556 | 8:-64655794 | None:intergenic |
| TCTTCCATTAATGGAGGAGT+TGG | 0.482425 | 8:+64655679 | MsG0880045800.01.T01:CDS |
| GGTTGGTGCAGTGGTGGTGG+TGG | 0.494544 | 8:-64655764 | None:intergenic |
| AAGAACTGTACCAGAAACTA+AGG | 0.497513 | 8:+64656421 | MsG0880045800.01.T01:CDS |
| TGGAAGATCAGACCAAGTTA+GGG | 0.500768 | 8:-64656616 | None:intergenic |
| CTGGTGGTGAATTAGGGTAC+TGG | 0.503929 | 8:+64656580 | MsG0880045800.01.T01:CDS |
| TTGGGTTTGAATTGCACCAT+TGG | 0.506743 | 8:-64656643 | None:intergenic |
| TCAACAACATCATCATCATC+AGG | 0.508236 | 8:+64656447 | MsG0880045800.01.T01:CDS |
| GTGTTTGATGAGTCACATCT+TGG | 0.511366 | 8:-64655841 | None:intergenic |
| ATCCAAGAACTCTTTGGTAA+TGG | 0.516650 | 8:+64655619 | MsG0880045800.01.T01:CDS |
| TTGGACACCTTAGATGTGGT+TGG | 0.518935 | 8:-64655822 | None:intergenic |
| GACGGTGGTTGGTGCAGTGG+TGG | 0.519460 | 8:-64655770 | None:intergenic |
| TGAAGTTGTCTCCAAAATGT+TGG | 0.525052 | 8:-64656553 | None:intergenic |
| TGAATTGCACCATTGGTAGT+TGG | 0.526899 | 8:-64656636 | None:intergenic |
| ACCACAAAGTCAAATCCTCT+GGG | 0.532491 | 8:+64656113 | MsG0880045800.01.T01:CDS |
| AAGAAGGCAATAACTTCATA+GGG | 0.534937 | 8:+64656214 | MsG0880045800.01.T01:CDS |
| TTCCGACAGCCACCTCCTAT+GGG | 0.538444 | 8:-64655964 | None:intergenic |
| CCTAGTAACACTGTTGGTGT+TGG | 0.540794 | 8:-64656501 | None:intergenic |
| GGAAGGTCAACAAGTGGTCA+AGG | 0.546021 | 8:+64656066 | MsG0880045800.01.T01:CDS |
| GTTTCTTTCACTTGAAGTGA+GGG | 0.555832 | 8:-64655653 | None:intergenic |
| GGTGACGGTGGTTGGTGCAG+TGG | 0.560684 | 8:-64655773 | None:intergenic |
| TTGAGGAACGTTCCCATAGG+AGG | 0.560915 | 8:+64655952 | MsG0880045800.01.T01:CDS |
| CAGAGGATTTGACTTTGTGG+TGG | 0.563970 | 8:-64656111 | None:intergenic |
| AGGAACGTTCCCATAGGAGG+TGG | 0.565496 | 8:+64655955 | MsG0880045800.01.T01:CDS |
| AGATCAGACCAAGTTAGGGT+TGG | 0.572219 | 8:-64656612 | None:intergenic |
| TCAAATGGTTATGATGGTGT+TGG | 0.575378 | 8:+64656279 | MsG0880045800.01.T01:CDS |
| AGTTTCTTTCACTTGAAGTG+AGG | 0.580149 | 8:-64655654 | None:intergenic |
| CAACACCAACAGTGTTACTA+GGG | 0.580552 | 8:+64656502 | MsG0880045800.01.T01:CDS |
| AAATGGTGGATTTGTACTTG+GGG | 0.583095 | 8:+64656362 | MsG0880045800.01.T01:CDS |
| TTCCCATAGGAGGTGGCTGT+CGG | 0.589355 | 8:+64655962 | MsG0880045800.01.T01:CDS |
| ACTCAACAATTACAAAATGG+TGG | 0.590751 | 8:+64656348 | MsG0880045800.01.T01:CDS |
| TCTGATCTTCCAACTACCAA+TGG | 0.595789 | 8:+64656627 | MsG0880045800.01.T01:CDS |
| TTTCTTCAGAGTTTAATGGA+AGG | 0.603721 | 8:+64656049 | MsG0880045800.01.T01:CDS |
| AACTCATCATGTGAGAAACA+TGG | 0.610751 | 8:-64656239 | None:intergenic |
| GAAGGTCAACAAGTGGTCAA+GGG | 0.612566 | 8:+64656067 | MsG0880045800.01.T01:CDS |
| CGTCGTTACTGGACAAAAGG+AGG | 0.618133 | 8:+64655925 | MsG0880045800.01.T01:CDS |
| GCTTTGAGGAACGTTCCCAT+AGG | 0.619952 | 8:+64655949 | MsG0880045800.01.T01:CDS |
| GAAGATGGGCGTGAAGAAGG+AGG | 0.621383 | 8:-64655730 | None:intergenic |
| CATCTTGGACACCTTAGATG+TGG | 0.628308 | 8:-64655826 | None:intergenic |
| AACTACTCTTCCATTAATGG+AGG | 0.630490 | 8:+64655673 | MsG0880045800.01.T01:CDS |
| TAACCATTTGACGAAATCAA+CGG | 0.644257 | 8:-64656267 | None:intergenic |
| GAGACAACTTCAGTTACTGG+TGG | 0.647714 | 8:+64656564 | MsG0880045800.01.T01:CDS |
| CATGTCTTGCAGAAATGGCG+AGG | 0.648175 | 8:-64655901 | None:intergenic |
| CATCACCATCATCAAAACAG+TGG | 0.654071 | 8:+64656393 | MsG0880045800.01.T01:CDS |
| ACTCATCATGTGAGAAACAT+GGG | 0.656250 | 8:-64656238 | None:intergenic |
| GGTGGTGGTTGTGGTGACGG+TGG | 0.663797 | 8:-64655785 | None:intergenic |
| TTTAATGGAAGGTCAACAAG+TGG | 0.666542 | 8:+64656060 | MsG0880045800.01.T01:CDS |
| AACAGTGTTACTAGGGAACG+TGG | 0.681785 | 8:+64656509 | MsG0880045800.01.T01:CDS |
| ACGTTCCCTAGTAACACTGT+TGG | 0.689714 | 8:-64656507 | None:intergenic |
| TATCAACGCCTCAAATAGTG+CGG | 0.693402 | 8:+64655996 | MsG0880045800.01.T01:CDS |
| AGAAATGGCGAGGCTGAGTG+AGG | 0.701300 | 8:-64655891 | None:intergenic |
| CCACAAAGTCAAATCCTCTG+GGG | 0.739195 | 8:+64656114 | MsG0880045800.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAACTCAACAATTACAAAA+TGG | + | Chr8:64656345-64656364 | MsG0880045800.01.T01:CDS | 25.0% |
| !!! | GAAAGTATATGAGAATTTTG+AGG | - | Chr8:64656144-64656163 | None:intergenic | 25.0% |
| AAGAAGGCAATAACTTCATA+GGG | + | Chr8:64656214-64656233 | MsG0880045800.01.T01:CDS | 30.0% | |
| ACAGTTTCTTCAGAGTTTAA+TGG | + | Chr8:64656045-64656064 | MsG0880045800.01.T01:CDS | 30.0% | |
| ACTCAACAATTACAAAATGG+TGG | + | Chr8:64656348-64656367 | MsG0880045800.01.T01:CDS | 30.0% | |
| AGAAACTACTCTTCCATTAA+TGG | + | Chr8:64655670-64655689 | MsG0880045800.01.T01:CDS | 30.0% | |
| ATTTCGTCAAATGGTTATGA+TGG | + | Chr8:64656273-64656292 | MsG0880045800.01.T01:CDS | 30.0% | |
| CTTCTTCCTTAACATGATTA+GGG | - | Chr8:64656200-64656219 | None:intergenic | 30.0% | |
| TAACCATTTGACGAAATCAA+CGG | - | Chr8:64656270-64656289 | None:intergenic | 30.0% | |
| TTTCTTCAGAGTTTAATGGA+AGG | + | Chr8:64656049-64656068 | MsG0880045800.01.T01:CDS | 30.0% | |
| ! | ATGTTTTATGCAACCCATTT+TGG | + | Chr8:64656304-64656323 | MsG0880045800.01.T01:CDS | 30.0% |
| ! | CATGATTGTTTCTCCAAAAT+GGG | - | Chr8:64656320-64656339 | None:intergenic | 30.0% |
| ! | TCATGATTGTTTCTCCAAAA+TGG | - | Chr8:64656321-64656340 | None:intergenic | 30.0% |
| ! | TTCCTCTATATCCAACATTT+TGG | + | Chr8:64656542-64656561 | MsG0880045800.01.T01:CDS | 30.0% |
| !! | AAAATGGTGGATTTGTACTT+GGG | + | Chr8:64656361-64656380 | MsG0880045800.01.T01:CDS | 30.0% |
| AACTACTCTTCCATTAATGG+AGG | + | Chr8:64655673-64655692 | MsG0880045800.01.T01:CDS | 35.0% | |
| AACTCATCATGTGAGAAACA+TGG | - | Chr8:64656242-64656261 | None:intergenic | 35.0% | |
| AAGAACTGTACCAGAAACTA+AGG | + | Chr8:64656421-64656440 | MsG0880045800.01.T01:CDS | 35.0% | |
| ACTCATCATGTGAGAAACAT+GGG | - | Chr8:64656241-64656260 | None:intergenic | 35.0% | |
| AGTTTCTTTCACTTGAAGTG+AGG | - | Chr8:64655657-64655676 | None:intergenic | 35.0% | |
| ATCCAAGAACTCTTTGGTAA+TGG | + | Chr8:64655619-64655638 | MsG0880045800.01.T01:CDS | 35.0% | |
| CCTAATCATGTTAAGGAAGA+AGG | + | Chr8:64656198-64656217 | MsG0880045800.01.T01:CDS | 35.0% | |
| CCTTAACATGATTAGGGTTA+GGG | - | Chr8:64656194-64656213 | None:intergenic | 35.0% | |
| CCTTCTTCCTTAACATGATT+AGG | - | Chr8:64656201-64656220 | None:intergenic | 35.0% | |
| CTCCAAAATGTTGGATATAG+AGG | - | Chr8:64656547-64656566 | None:intergenic | 35.0% | |
| GAAGAAGGCAATAACTTCAT+AGG | + | Chr8:64656213-64656232 | MsG0880045800.01.T01:CDS | 35.0% | |
| GTTTCTTTCACTTGAAGTGA+GGG | - | Chr8:64655656-64655675 | None:intergenic | 35.0% | |
| TCAACAACATCATCATCATC+AGG | + | Chr8:64656447-64656466 | MsG0880045800.01.T01:CDS | 35.0% | |
| TCCTTAACATGATTAGGGTT+AGG | - | Chr8:64656195-64656214 | None:intergenic | 35.0% | |
| TGAAGTTGTCTCCAAAATGT+TGG | - | Chr8:64656556-64656575 | None:intergenic | 35.0% | |
| TTTAATGGAAGGTCAACAAG+TGG | + | Chr8:64656060-64656079 | MsG0880045800.01.T01:CDS | 35.0% | |
| ! | TCAAATGGTTATGATGGTGT+TGG | + | Chr8:64656279-64656298 | MsG0880045800.01.T01:CDS | 35.0% |
| !! | AAATGGTGGATTTGTACTTG+GGG | + | Chr8:64656362-64656381 | MsG0880045800.01.T01:CDS | 35.0% |
| !! | CAAAATGGTGGATTTGTACT+TGG | + | Chr8:64656360-64656379 | MsG0880045800.01.T01:CDS | 35.0% |
| !! | TTAGTTTCTGGTACAGTTCT+TGG | - | Chr8:64656422-64656441 | None:intergenic | 35.0% |
| !! | TTGTTGATGACCTTAGTTTC+TGG | - | Chr8:64656434-64656453 | None:intergenic | 35.0% |
| ACCACAAAGTCAAATCCTCT+GGG | + | Chr8:64656113-64656132 | MsG0880045800.01.T01:CDS | 40.0% | |
| ATCCAATTCCAACCCTAACT+TGG | + | Chr8:64656604-64656623 | MsG0880045800.01.T01:CDS | 40.0% | |
| CACCATTACCAAAGAGTTCT+TGG | - | Chr8:64655624-64655643 | None:intergenic | 40.0% | |
| CAGAAAACCAACCACATCTA+AGG | + | Chr8:64655815-64655834 | MsG0880045800.01.T01:CDS | 40.0% | |
| CATCACCATCATCAAAACAG+TGG | + | Chr8:64656393-64656412 | MsG0880045800.01.T01:CDS | 40.0% | |
| CCCTAACCCTAATCATGTTA+AGG | + | Chr8:64656191-64656210 | MsG0880045800.01.T01:CDS | 40.0% | |
| GTGTTTGATGAGTCACATCT+TGG | - | Chr8:64655844-64655863 | None:intergenic | 40.0% | |
| TATCAACGCCTCAAATAGTG+CGG | + | Chr8:64655996-64656015 | MsG0880045800.01.T01:CDS | 40.0% | |
| TCTGATCTTCCAACTACCAA+TGG | + | Chr8:64656627-64656646 | MsG0880045800.01.T01:CDS | 40.0% | |
| TCTTCCATTAATGGAGGAGT+TGG | + | Chr8:64655679-64655698 | MsG0880045800.01.T01:CDS | 40.0% | |
| TGGAAGATCAGACCAAGTTA+GGG | - | Chr8:64656619-64656638 | None:intergenic | 40.0% | |
| TTGGAAGATCAGACCAAGTT+AGG | - | Chr8:64656620-64656639 | None:intergenic | 40.0% | |
| TTGGAGACAACTTCAGTTAC+TGG | + | Chr8:64656561-64656580 | MsG0880045800.01.T01:CDS | 40.0% | |
| TTGGGTTTGAATTGCACCAT+TGG | - | Chr8:64656646-64656665 | None:intergenic | 40.0% | |
| ! | CAACACCAACAGTGTTACTA+GGG | + | Chr8:64656502-64656521 | MsG0880045800.01.T01:CDS | 40.0% |
| ! | CAGTTACTGGTGGTGAATTA+GGG | + | Chr8:64656574-64656593 | MsG0880045800.01.T01:CDS | 40.0% |
| ! | CATGATTAGGGTTAGGGTTT+TGG | - | Chr8:64656188-64656207 | None:intergenic | 40.0% |
| ! | TCAGTTACTGGTGGTGAATT+AGG | + | Chr8:64656573-64656592 | MsG0880045800.01.T01:CDS | 40.0% |
| ! | TGAATTGCACCATTGGTAGT+TGG | - | Chr8:64656639-64656658 | None:intergenic | 40.0% |
| !! | TGTTTTTGCCGCACTATTTG+AGG | - | Chr8:64656007-64656026 | None:intergenic | 40.0% |
| !!! | GGATTCCACTGTTTTGATGA+TGG | - | Chr8:64656401-64656420 | None:intergenic | 40.0% |
| !!! | TGGTTGGTTTTCTGATGATG+CGG | - | Chr8:64655809-64655828 | None:intergenic | 40.0% |
| ACGTTCCCTAGTAACACTGT+TGG | - | Chr8:64656510-64656529 | None:intergenic | 45.0% | |
| AGATCAGACCAAGTTAGGGT+TGG | - | Chr8:64656615-64656634 | None:intergenic | 45.0% | |
| CACCACAAAGTCAAATCCTC+TGG | + | Chr8:64656112-64656131 | MsG0880045800.01.T01:CDS | 45.0% | |
| CATCTTGGACACCTTAGATG+TGG | - | Chr8:64655829-64655848 | None:intergenic | 45.0% | |
| CCACAAAGTCAAATCCTCTG+GGG | + | Chr8:64656114-64656133 | MsG0880045800.01.T01:CDS | 45.0% | |
| CCTAGTAACACTGTTGGTGT+TGG | - | Chr8:64656504-64656523 | None:intergenic | 45.0% | |
| GAAGAAGGAGGTTGTGTTGT+TGG | - | Chr8:64655721-64655740 | None:intergenic | 45.0% | |
| GAAGGTCAACAAGTGGTCAA+GGG | + | Chr8:64656067-64656086 | MsG0880045800.01.T01:CDS | 45.0% | |
| GACCAAGTTAGGGTTGGAAT+TGG | - | Chr8:64656609-64656628 | None:intergenic | 45.0% | |
| GAGACAACTTCAGTTACTGG+TGG | + | Chr8:64656564-64656583 | MsG0880045800.01.T01:CDS | 45.0% | |
| TCCATTAATGGAGGAGTTGG+AGG | + | Chr8:64655682-64655701 | MsG0880045800.01.T01:CDS | 45.0% | |
| TCCTCCAACTCCTCCATTAA+TGG | - | Chr8:64655686-64655705 | None:intergenic | 45.0% | |
| TTGGACACCTTAGATGTGGT+TGG | - | Chr8:64655825-64655844 | None:intergenic | 45.0% | |
| ! | AACAGTGTTACTAGGGAACG+TGG | + | Chr8:64656509-64656528 | MsG0880045800.01.T01:CDS | 45.0% |
| ! | CAGAGGATTTGACTTTGTGG+TGG | - | Chr8:64656114-64656133 | None:intergenic | 45.0% |
| ! | CCAACACCAACAGTGTTACT+AGG | + | Chr8:64656501-64656520 | MsG0880045800.01.T01:CDS | 45.0% |
| ! | CTCCTTTTGTCCAGTAACGA+CGG | - | Chr8:64655927-64655946 | None:intergenic | 45.0% |
| !! | AATTTTGAGGAGAACCCCAG+AGG | - | Chr8:64656131-64656150 | None:intergenic | 45.0% |
| !! | CAGCCGTTGATTTCGTCAAA+TGG | + | Chr8:64656264-64656283 | MsG0880045800.01.T01:CDS | 45.0% |
| !! | TTGGTTTTCTGATGATGCGG+TGG | - | Chr8:64655806-64655825 | None:intergenic | 45.0% |
| CATGTCTTGCAGAAATGGCG+AGG | - | Chr8:64655904-64655923 | None:intergenic | 50.0% | |
| CGTCGTTACTGGACAAAAGG+AGG | + | Chr8:64655925-64655944 | MsG0880045800.01.T01:CDS | 50.0% | |
| GACGGCATGTCTTGCAGAAA+TGG | - | Chr8:64655909-64655928 | None:intergenic | 50.0% | |
| GGAAGGTCAACAAGTGGTCA+AGG | + | Chr8:64656066-64656085 | MsG0880045800.01.T01:CDS | 50.0% | |
| TGCCGTCGTTACTGGACAAA+AGG | + | Chr8:64655922-64655941 | MsG0880045800.01.T01:CDS | 50.0% | |
| TTGAGGAACGTTCCCATAGG+AGG | + | Chr8:64655952-64655971 | MsG0880045800.01.T01:CDS | 50.0% | |
| ! | CCCCAGAGGATTTGACTTTG+TGG | - | Chr8:64656117-64656136 | None:intergenic | 50.0% |
| ! | CTGGTGGTGAATTAGGGTAC+TGG | + | Chr8:64656580-64656599 | MsG0880045800.01.T01:CDS | 50.0% |
| !! | GCTTTGAGGAACGTTCCCAT+AGG | + | Chr8:64655949-64655968 | MsG0880045800.01.T01:CDS | 50.0% |
| !! | GGACAAAAGGAGGTGCTTTG+AGG | + | Chr8:64655935-64655954 | MsG0880045800.01.T01:CDS | 50.0% |
| !! | GTTTTCTGATGATGCGGTGG+TGG | - | Chr8:64655803-64655822 | None:intergenic | 50.0% |
| !! | TGGTGGTGGTTGTGGAAGAT+GGG | - | Chr8:64655747-64655766 | None:intergenic | 50.0% |
| AGAAATGGCGAGGCTGAGTG+AGG | - | Chr8:64655894-64655913 | None:intergenic | 55.0% | |
| AGGAACGTTCCCATAGGAGG+TGG | + | Chr8:64655955-64655974 | MsG0880045800.01.T01:CDS | 55.0% | |
| GAAGATGGGCGTGAAGAAGG+AGG | - | Chr8:64655733-64655752 | None:intergenic | 55.0% | |
| GCAAGACATGCCGTCGTTAC+TGG | + | Chr8:64655914-64655933 | MsG0880045800.01.T01:CDS | 55.0% | |
| GTGGAAGATGGGCGTGAAGA+AGG | - | Chr8:64655736-64655755 | None:intergenic | 55.0% | |
| TTCCCATAGGAGGTGGCTGT+CGG | + | Chr8:64655962-64655981 | MsG0880045800.01.T01:CDS | 55.0% | |
| TTCCGACAGCCACCTCCTAT+GGG | - | Chr8:64655967-64655986 | None:intergenic | 55.0% | |
| TTTCCGACAGCCACCTCCTA+TGG | - | Chr8:64655968-64655987 | None:intergenic | 55.0% | |
| ! | TGATGATGCGGTGGTGGTTG+TGG | - | Chr8:64655797-64655816 | None:intergenic | 55.0% |
| !! | GTGGTGGTGGTTGTGGAAGA+TGG | - | Chr8:64655748-64655767 | None:intergenic | 55.0% |
| ! | TGCGGTGGTGGTTGTGGTGA+CGG | - | Chr8:64655791-64655810 | None:intergenic | 60.0% |
| !! | GTGGTTGTGGTGACGGTGGT+TGG | - | Chr8:64655784-64655803 | None:intergenic | 60.0% |
| ! | GACGGTGGTTGGTGCAGTGG+TGG | - | Chr8:64655773-64655792 | None:intergenic | 65.0% |
| ! | GGTGACGGTGGTTGGTGCAG+TGG | - | Chr8:64655776-64655795 | None:intergenic | 65.0% |
| ! | GGTGGTTGGTGCAGTGGTGG+TGG | - | Chr8:64655770-64655789 | None:intergenic | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTTG+TGG | - | Chr8:64655755-64655774 | None:intergenic | 65.0% |
| !! | GGTGGTGGTTGTGGTGACGG+TGG | - | Chr8:64655788-64655807 | None:intergenic | 65.0% |
| !! | GGTTGGTGCAGTGGTGGTGG+TGG | - | Chr8:64655767-64655786 | None:intergenic | 65.0% |
| !! | TGCAGTGGTGGTGGTGGTGG+TGG | - | Chr8:64655761-64655780 | None:intergenic | 65.0% |
| !! | TGGTGCAGTGGTGGTGGTGG+TGG | - | Chr8:64655764-64655783 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 64655616 | 64656674 | 64655616 | ID=MsG0880045800.01;Name=MsG0880045800.01 |
| Chr8 | mRNA | 64655616 | 64656674 | 64655616 | ID=MsG0880045800.01.T01;Parent=MsG0880045800.01;Name=MsG0880045800.01.T01;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|352 |
| Chr8 | exon | 64655616 | 64656674 | 64655616 | ID=MsG0880045800.01.T01:exon:24496;Parent=MsG0880045800.01.T01 |
| Chr8 | CDS | 64655616 | 64656674 | 64655616 | ID=MsG0880045800.01.T01:cds;Parent=MsG0880045800.01.T01 |
| Gene Sequence |
| Protein sequence |
