Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045947.01.T01 | AFK48687.1 | 98.734 | 79 | 1 | 0 | 40 | 118 | 27 | 105 | 9.83E-48 | 158 |
| MsG0880045947.01.T02 | AFK48687.1 | 100 | 30 | 0 | 0 | 1 | 30 | 76 | 105 | 1.97E-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045947.01.T01 | Q10J20 | 74.359 | 78 | 20 | 0 | 40 | 117 | 193 | 270 | 2.17E-36 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045947.01.T01 | A0A396IBL5 | 96.296 | 81 | 3 | 0 | 38 | 118 | 139 | 219 | 4.65e-47 | 160 |
| MsG0880045947.01.T01 | I3T845 | 98.734 | 79 | 1 | 0 | 40 | 118 | 27 | 105 | 4.69e-48 | 158 |
| MsG0880045947.01.T02 | I3T845 | 100.000 | 30 | 0 | 0 | 1 | 30 | 76 | 105 | 9.40e-12 | 62.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045947.01 | MsG0880045960.01 | 0.827719 | 1.339571e-54 | 1.303831e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045947.01.T01 | MTR_4g095310 | 96.296 | 81 | 3 | 0 | 38 | 118 | 190 | 270 | 9.33e-50 | 159 |
| MsG0880045947.01.T01 | MTR_5g016150 | 75.000 | 76 | 19 | 0 | 41 | 116 | 193 | 268 | 9.79e-37 | 127 |
| MsG0880045947.01.T01 | MTR_1g018320 | 50.820 | 61 | 30 | 0 | 41 | 101 | 195 | 255 | 1.97e-17 | 75.5 |
| MsG0880045947.01.T02 | MTR_4g095310 | 100.000 | 30 | 0 | 0 | 1 | 30 | 241 | 270 | 2.20e-14 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045947.01.T01 | AT4G37470 | 76.623 | 77 | 18 | 0 | 41 | 117 | 193 | 269 | 2.35e-36 | 125 |
| MsG0880045947.01.T01 | AT3G03990 | 54.098 | 61 | 28 | 0 | 41 | 101 | 194 | 254 | 1.95e-18 | 78.2 |
| MsG0880045947.01.T01 | AT3G24420 | 44.262 | 61 | 33 | 1 | 41 | 100 | 199 | 259 | 1.25e-11 | 60.1 |
Find 34 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGCCGGACGTTGTTATTC+CGG | 0.318614 | 8:-66794346 | MsG0880045947.01.T01:CDS |
| AATTACAAATCATGGTGTTC+TGG | 0.329664 | 8:-66794653 | None:intergenic |
| AAAATGTTTGTAACACGCTT+AGG | 0.334974 | 8:+66794540 | None:intergenic |
| AGATATTCCGCGACCACGAC+TGG | 0.422541 | 8:+66794438 | None:intergenic |
| AAATGTTTGTAACACGCTTA+GGG | 0.443133 | 8:+66794541 | None:intergenic |
| GGATTTGCTCCAATGGCAGT+TGG | 0.462285 | 8:-66794632 | MsG0880045947.01.T01:CDS |
| ATGTCCCCTCCAACTGCCAT+TGG | 0.469708 | 8:+66794623 | None:intergenic |
| TGCTCCAATGGCAGTTGGAG+GGG | 0.510556 | 8:-66794627 | MsG0880045947.01.T01:CDS |
| CCTTCATACTTTGTATAATG+TGG | 0.518604 | 8:+66794472 | None:intergenic |
| TACTTTGTATAATGTGGCAT+GGG | 0.519899 | 8:+66794478 | None:intergenic |
| AATGGCAGTTGGAGGGGACA+TGG | 0.527300 | 8:-66794621 | MsG0880045947.01.T01:CDS |
| GTGTTCTGGATTTGCTCCAA+TGG | 0.532527 | 8:-66794639 | MsG0880045947.01.T01:CDS |
| AGCGGTTCCAGTCGTGGTCG+CGG | 0.535585 | 8:-66794445 | MsG0880045947.01.T01:CDS |
| ATCACCGGAATAACAACGTC+CGG | 0.537857 | 8:+66794342 | None:intergenic |
| CCACATTATACAAAGTATGA+AGG | 0.538729 | 8:-66794472 | MsG0880045947.01.T01:CDS |
| TTTGCTCCAATGGCAGTTGG+AGG | 0.538790 | 8:-66794629 | MsG0880045947.01.T01:CDS |
| ATACTTTGTATAATGTGGCA+TGG | 0.539342 | 8:+66794477 | None:intergenic |
| TTGCTCCAATGGCAGTTGGA+GGG | 0.545271 | 8:-66794628 | MsG0880045947.01.T01:CDS |
| CGAATGTGTTTGAGTATCAC+CGG | 0.546328 | 8:+66794327 | None:intergenic |
| AACACGCTTAGGGCTATGTC+TGG | 0.562881 | 8:+66794551 | None:intergenic |
| ACTCGCTACCAACATGTTGG+TGG | 0.576429 | 8:+66794415 | None:intergenic |
| GGATCTAGCGGTTCCAGTCG+TGG | 0.576985 | 8:-66794451 | MsG0880045947.01.T01:CDS |
| ACACATTCGTCATGATATTG+CGG | 0.602861 | 8:-66794313 | MsG0880045947.01.T01:CDS |
| GAATATCTCCACCAACATGT+TGG | 0.605480 | 8:-66794423 | MsG0880045947.01.T01:CDS |
| TGGACTCGCTACCAACATGT+TGG | 0.613943 | 8:+66794412 | None:intergenic |
| CATGAGACAAATATTATGCA+TGG | 0.615267 | 8:-66794508 | MsG0880045947.01.T01:CDS |
| TTTGCCGCAATTAAGCTCGC+CGG | 0.620386 | 8:-66794361 | MsG0880045947.01.T01:CDS |
| ACGTCCGGCGAGCTTAATTG+CGG | 0.647186 | 8:+66794357 | None:intergenic |
| GTGGAGGTTATGTCGACAGA+GGG | 0.648761 | 8:-66794387 | MsG0880045947.01.T01:CDS |
| ACAAAGTATGAAGGATCTAG+CGG | 0.659829 | 8:-66794463 | MsG0880045947.01.T01:CDS |
| CGTGGAGGTTATGTCGACAG+AGG | 0.671230 | 8:-66794388 | MsG0880045947.01.T01:CDS |
| TGTTGGTAGCGAGTCCATCG+TGG | 0.709388 | 8:-66794406 | MsG0880045947.01.T01:CDS |
| TGGTAGCGAGTCCATCGTGG+AGG | 0.724029 | 8:-66794403 | MsG0880045947.01.T01:CDS |
| TGTCGACATAACCTCCACGA+TGG | 0.724152 | 8:+66794392 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGTTTGTAACACGCTT+AGG | + | Chr8:66794413-66794432 | None:intergenic | 30.0% | |
| AAATGTTTGTAACACGCTTA+GGG | + | Chr8:66794412-66794431 | None:intergenic | 30.0% | |
| ATACTTTGTATAATGTGGCA+TGG | + | Chr8:66794476-66794495 | None:intergenic | 30.0% | |
| CATGAGACAAATATTATGCA+TGG | - | Chr8:66794442-66794461 | MsG0880045947.01.T01:CDS | 30.0% | |
| CCACATTATACAAAGTATGA+AGG | - | Chr8:66794478-66794497 | MsG0880045947.01.T01:CDS | 30.0% | |
| CCTTCATACTTTGTATAATG+TGG | + | Chr8:66794481-66794500 | None:intergenic | 30.0% | |
| TACTTTGTATAATGTGGCAT+GGG | + | Chr8:66794475-66794494 | None:intergenic | 30.0% | |
| ACAAAGTATGAAGGATCTAG+CGG | - | Chr8:66794487-66794506 | MsG0880045947.01.T01:CDS | 35.0% | |
| ACACATTCGTCATGATATTG+CGG | - | Chr8:66794637-66794656 | MsG0880045947.01.T01:CDS | 35.0% | |
| CGAATGTGTTTGAGTATCAC+CGG | + | Chr8:66794626-66794645 | None:intergenic | 40.0% | |
| GAATATCTCCACCAACATGT+TGG | - | Chr8:66794527-66794546 | MsG0880045947.01.T01:CDS | 40.0% | |
| ATCACCGGAATAACAACGTC+CGG | + | Chr8:66794611-66794630 | None:intergenic | 45.0% | |
| ! | GTGTTCTGGATTTGCTCCAA+TGG | - | Chr8:66794311-66794330 | MsG0880045947.01.T01:CDS | 45.0% |
| AACACGCTTAGGGCTATGTC+TGG | + | Chr8:66794402-66794421 | None:intergenic | 50.0% | |
| ACTCGCTACCAACATGTTGG+TGG | + | Chr8:66794538-66794557 | None:intergenic | 50.0% | |
| GTGGAGGTTATGTCGACAGA+GGG | - | Chr8:66794563-66794582 | MsG0880045947.01.T01:CDS | 50.0% | |
| TGGACTCGCTACCAACATGT+TGG | + | Chr8:66794541-66794560 | None:intergenic | 50.0% | |
| TGTCGACATAACCTCCACGA+TGG | + | Chr8:66794561-66794580 | None:intergenic | 50.0% | |
| TTGCTCCAATGGCAGTTGGA+GGG | - | Chr8:66794322-66794341 | MsG0880045947.01.T01:CDS | 50.0% | |
| TTTGCCGCAATTAAGCTCGC+CGG | - | Chr8:66794589-66794608 | MsG0880045947.01.T01:CDS | 50.0% | |
| TTTGCTCCAATGGCAGTTGG+AGG | - | Chr8:66794321-66794340 | MsG0880045947.01.T01:CDS | 50.0% | |
| ! | GGATTTGCTCCAATGGCAGT+TGG | - | Chr8:66794318-66794337 | MsG0880045947.01.T01:CDS | 50.0% |
| AATGGCAGTTGGAGGGGACA+TGG | - | Chr8:66794329-66794348 | MsG0880045947.01.T01:CDS | 55.0% | |
| ACGTCCGGCGAGCTTAATTG+CGG | + | Chr8:66794596-66794615 | None:intergenic | 55.0% | |
| AGATATTCCGCGACCACGAC+TGG | + | Chr8:66794515-66794534 | None:intergenic | 55.0% | |
| ATGTCCCCTCCAACTGCCAT+TGG | + | Chr8:66794330-66794349 | None:intergenic | 55.0% | |
| CGTGGAGGTTATGTCGACAG+AGG | - | Chr8:66794562-66794581 | MsG0880045947.01.T01:CDS | 55.0% | |
| TGCTCCAATGGCAGTTGGAG+GGG | - | Chr8:66794323-66794342 | MsG0880045947.01.T01:CDS | 55.0% | |
| ! | CTCGCCGGACGTTGTTATTC+CGG | - | Chr8:66794604-66794623 | MsG0880045947.01.T01:CDS | 55.0% |
| !! | TGTTGGTAGCGAGTCCATCG+TGG | - | Chr8:66794544-66794563 | MsG0880045947.01.T01:CDS | 55.0% |
| GGATCTAGCGGTTCCAGTCG+TGG | - | Chr8:66794499-66794518 | MsG0880045947.01.T01:CDS | 60.0% | |
| ! | TGGTAGCGAGTCCATCGTGG+AGG | - | Chr8:66794547-66794566 | MsG0880045947.01.T01:CDS | 60.0% |
| AGCGGTTCCAGTCGTGGTCG+CGG | - | Chr8:66794505-66794524 | MsG0880045947.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 66794308 | 66794664 | 66794308 | ID=MsG0880045947.01;Name=MsG0880045947.01 |
| Chr8 | mRNA | 66794308 | 66794664 | 66794308 | ID=MsG0880045947.01.T01;Parent=MsG0880045947.01;Name=MsG0880045947.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|118 |
| Chr8 | exon | 66794308 | 66794664 | 66794308 | ID=MsG0880045947.01.T01:exon:13899;Parent=MsG0880045947.01.T01 |
| Chr8 | CDS | 66794308 | 66794664 | 66794308 | ID=MsG0880045947.01.T01:cds;Parent=MsG0880045947.01.T01 |
| Chr8 | mRNA | 66794308 | 66794400 | 66794308 | ID=MsG0880045947.01.T02;Parent=MsG0880045947.01;Name=MsG0880045947.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|30 |
| Chr8 | exon | 66794308 | 66794400 | 66794308 | ID=MsG0880045947.01.T02:exon:13900;Parent=MsG0880045947.01.T02 |
| Chr8 | CDS | 66794308 | 66794400 | 66794308 | ID=MsG0880045947.01.T02:cds;Parent=MsG0880045947.01.T02 |
| Gene Sequence |
| Protein sequence |
