AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045975.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045975.01.T01 MTR_4g095500 93.521 355 3 1 1 335 116 470 0.0 680
MsG0880045975.01.T01 MTR_5g015950 73.623 345 73 4 9 335 104 448 0.0 519
MsG0880045975.01.T01 MTR_3g053270 73.623 345 73 4 9 335 104 448 0.0 519
MsG0880045975.01.T01 MTR_2g089100 44.675 338 167 4 12 335 114 445 5.82e-99 300
MsG0880045975.01.T01 MTR_4g097080 31.746 315 189 6 26 334 210 504 1.10e-46 165
MsG0880045975.01.T01 MTR_5g015490 29.518 332 213 6 8 334 176 491 5.10e-44 158
MsG0880045975.01.T01 MTR_3g089055 30.363 303 195 4 31 332 277 564 1.49e-38 144
MsG0880045975.01.T01 MTR_3g089055 30.363 303 195 4 31 332 282 569 1.68e-38 144
MsG0880045975.01.T01 MTR_2g099110 30.499 341 195 10 10 334 138 452 3.66e-38 141
MsG0880045975.01.T01 MTR_1g029420 30.693 303 194 5 31 332 304 591 1.40e-37 142
MsG0880045975.01.T01 MTR_4g133660 30.844 308 187 6 32 333 256 543 4.73e-37 139
MsG0880045975.01.T01 MTR_8g442410 29.967 307 182 11 33 332 244 524 4.25e-35 134
MsG0880045975.01.T01 MTR_5g097480 30.195 308 189 7 31 332 256 543 2.12e-34 132
MsG0880045975.01.T01 MTR_2g082090 28.065 310 197 5 29 332 289 578 3.58e-32 126
MsG0880045975.01.T01 MTR_2g026250 29.470 302 197 6 32 332 309 595 6.74e-32 125
MsG0880045975.01.T01 MTR_3g056110 27.632 304 202 6 31 332 254 541 8.09e-32 125
MsG0880045975.01.T01 MTR_5g094450 27.723 303 204 6 31 332 243 531 2.56e-31 124
MsG0880045975.01.T01 MTR_3g065980 27.586 348 210 10 2 332 217 539 9.84e-30 119
MsG0880045975.01.T01 MTR_8g020840 28.659 328 199 11 20 332 240 547 4.90e-29 117
MsG0880045975.01.T01 MTR_7g027190 26.791 321 205 8 15 332 379 672 4.96e-28 115
MsG0880045975.01.T01 MTR_3g022830 27.353 340 204 9 29 332 103 435 1.24e-25 106
MsG0880045975.01.T01 MTR_4g064150 25.163 306 211 7 33 335 445 735 1.40e-25 107
MsG0880045975.01.T01 MTR_4g064180 25.566 309 204 10 34 335 339 628 2.54e-24 103
MsG0880045975.01.T01 MTR_4g064120 25.163 306 209 8 34 335 339 628 4.95e-24 103
MsG0880045975.01.T01 MTR_4g104020 27.476 313 183 11 33 332 238 519 7.33e-24 102
MsG0880045975.01.T01 MTR_4g064200 25.321 312 211 8 29 335 358 652 1.71e-23 101
MsG0880045975.01.T01 MTR_6g047750 27.632 304 193 6 37 332 377 661 3.26e-22 97.8
MsG0880045975.01.T01 MTR_2g097467 24.194 310 216 7 29 335 363 656 3.73e-22 97.8
MsG0880045975.01.T01 MTR_7g057230 25.914 301 199 5 37 332 375 656 3.73e-22 97.8
MsG0880045975.01.T01 MTR_2g097410 23.301 309 221 7 29 335 449 743 5.63e-21 94.4
MsG0880045975.01.T01 MTR_2g097473 23.151 311 218 7 29 335 363 656 1.45e-20 93.2
MsG0880045975.01.T01 MTR_4g102790 22.508 311 209 8 32 334 377 663 2.18e-20 92.4
MsG0880045975.01.T01 MTR_4g064160 25.796 314 206 10 29 335 393 686 3.31e-20 92.0
MsG0880045975.01.T01 MTR_5g009080 23.352 364 199 8 29 332 134 477 5.23e-20 90.9
MsG0880045975.01.T01 MTR_2g097463 24.437 311 214 7 29 335 364 657 5.79e-20 91.3
MsG0880045975.01.T01 MTR_7g062120 23.718 312 215 7 29 335 449 742 5.84e-20 91.3
MsG0880045975.01.T01 MTR_4g076140 23.641 368 192 9 29 332 126 468 1.82e-19 89.4
MsG0880045975.01.T01 MTR_4g077760 27.302 315 186 13 31 332 270 554 1.50e-18 87.0
MsG0880045975.01.T01 MTR_2g097310 23.779 307 209 8 34 335 354 640 2.73e-18 86.3
MsG0880045975.01.T01 MTR_1g069725 25.392 319 204 12 19 332 173 462 3.37e-18 85.5
MsG0880045975.01.T01 MTR_7g074650 25.321 312 194 11 29 334 515 793 4.00e-17 82.8
MsG0880045975.01.T01 MTR_2g097350 22.801 307 212 7 34 335 356 642 3.54e-16 79.7
MsG0880045975.01.T01 MTR_5g058860 26.299 308 208 9 31 332 202 496 1.67e-15 77.8
MsG0880045975.01.T01 MTR_1g096030 23.304 339 200 13 25 332 149 458 2.45e-15 77.0
MsG0880045975.01.T01 MTR_8g093070 22.356 331 217 10 13 335 200 498 4.38e-15 76.3
MsG0880045975.01.T01 MTR_4g076020 24.194 310 192 10 28 335 163 431 5.32e-15 75.9
MsG0880045975.01.T01 MTR_3g072710 23.377 308 208 10 31 332 214 499 1.63e-14 74.7
MsG0880045975.01.T01 MTR_7g109580 21.192 302 213 7 34 332 274 553 6.74e-11 63.5
MsG0880045975.01.T02 MTR_4g095500 98.592 355 3 1 1 353 116 470 0.0 722
MsG0880045975.01.T02 MTR_5g015950 72.286 350 87 4 9 353 104 448 0.0 521
MsG0880045975.01.T02 MTR_3g053270 72.286 350 87 4 9 353 104 448 0.0 521
MsG0880045975.01.T02 MTR_2g089100 42.135 356 168 4 12 353 114 445 1.05e-95 292
MsG0880045975.01.T02 MTR_4g097080 30.030 333 189 7 26 352 210 504 3.39e-44 159
MsG0880045975.01.T02 MTR_5g015490 28.571 350 211 7 8 352 176 491 2.47e-41 151
MsG0880045975.01.T02 MTR_2g099110 28.691 359 196 10 10 352 138 452 4.81e-36 136
MsG0880045975.01.T02 MTR_3g089055 28.660 321 195 4 31 350 277 564 7.14e-36 137
MsG0880045975.01.T02 MTR_3g089055 28.660 321 195 4 31 350 282 569 7.76e-36 137
MsG0880045975.01.T02 MTR_1g029420 28.144 334 204 6 18 350 293 591 5.50e-35 135
MsG0880045975.01.T02 MTR_4g133660 29.141 326 187 6 32 351 256 543 1.40e-34 133
MsG0880045975.01.T02 MTR_8g442410 28.923 325 180 12 33 350 244 524 1.02e-32 128
MsG0880045975.01.T02 MTR_5g097480 28.528 326 189 7 31 350 256 543 5.45e-32 126
MsG0880045975.01.T02 MTR_2g082090 26.524 328 197 5 29 350 289 578 1.03e-29 120
MsG0880045975.01.T02 MTR_3g056110 26.087 322 202 6 31 350 254 541 1.75e-29 119
MsG0880045975.01.T02 MTR_2g026250 27.812 320 197 6 32 350 309 595 1.76e-29 119
MsG0880045975.01.T02 MTR_5g094450 26.168 321 204 6 31 350 243 531 3.28e-29 118
MsG0880045975.01.T02 MTR_3g065980 25.956 366 211 9 2 350 217 539 1.88e-27 113
MsG0880045975.01.T02 MTR_3g022830 25.141 354 212 8 29 350 103 435 1.81e-26 109
MsG0880045975.01.T02 MTR_7g027190 25.369 339 205 8 15 350 379 672 7.26e-26 109
MsG0880045975.01.T02 MTR_4g064150 24.074 324 210 7 33 353 445 735 4.67e-24 103
MsG0880045975.01.T02 MTR_4g064180 24.465 327 203 10 34 353 339 628 9.29e-23 99.8
MsG0880045975.01.T02 MTR_4g064120 24.074 324 208 8 34 353 339 628 1.78e-22 99.0
MsG0880045975.01.T02 MTR_4g064200 24.242 330 210 8 29 353 358 652 9.96e-22 97.1
MsG0880045975.01.T02 MTR_4g104020 25.982 331 183 11 33 350 238 519 1.06e-21 96.7
MsG0880045975.01.T02 MTR_4g076140 24.457 368 207 12 29 350 126 468 6.57e-21 94.0
MsG0880045975.01.T02 MTR_2g097467 22.866 328 216 7 29 353 363 656 2.94e-20 92.4
MsG0880045975.01.T02 MTR_6g047750 26.087 322 193 6 37 350 377 661 6.48e-20 91.7
MsG0880045975.01.T02 MTR_5g009080 23.352 364 217 8 29 350 134 477 6.58e-20 90.9
MsG0880045975.01.T02 MTR_7g057230 24.451 319 199 5 37 350 375 656 8.05e-20 91.3
MsG0880045975.01.T02 MTR_2g097410 22.324 327 220 7 29 353 449 743 2.40e-19 89.7
MsG0880045975.01.T02 MTR_2g097473 21.884 329 218 7 29 353 363 656 1.62e-18 87.4
MsG0880045975.01.T02 MTR_7g062120 22.769 325 210 7 34 353 454 742 1.94e-18 87.0
MsG0880045975.01.T02 MTR_2g097463 23.100 329 214 7 29 353 364 657 5.07e-18 85.9
MsG0880045975.01.T02 MTR_4g102790 21.884 329 207 10 32 352 377 663 1.76e-17 84.3
MsG0880045975.01.T02 MTR_2g097310 22.462 325 209 8 34 353 354 640 4.60e-16 79.7
MsG0880045975.01.T02 MTR_1g069725 24.779 339 199 13 19 350 173 462 5.02e-16 79.3
MsG0880045975.01.T02 MTR_4g077760 25.826 333 186 14 31 350 270 554 2.02e-15 77.8
MsG0880045975.01.T02 MTR_7g074650 23.939 330 194 11 29 352 515 793 5.91e-15 76.6
MsG0880045975.01.T02 MTR_2g097390 22.154 325 208 9 34 352 357 642 1.20e-14 75.5
MsG0880045975.01.T02 MTR_2g097350 21.538 325 212 7 34 353 356 642 5.17e-14 73.6
MsG0880045975.01.T02 MTR_5g058860 25.382 327 205 11 31 350 202 496 7.63e-14 72.8
MsG0880045975.01.T02 MTR_4g076020 24.074 324 185 12 32 353 167 431 3.89e-13 70.5
MsG0880045975.01.T02 MTR_3g072710 22.393 326 207 11 31 350 214 499 1.06e-12 69.3
MsG0880045975.01.T02 MTR_8g093070 20.977 348 217 10 13 352 200 497 1.97e-12 68.6
MsG0880045975.01.T02 MTR_2g097380 21.605 324 198 9 34 353 292 563 2.82e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045975.01.T01 AT3G49950 62.112 322 104 6 19 334 100 409 3.36e-143 411
MsG0880045975.01.T01 AT5G67411 60.766 209 73 4 68 274 1 202 8.56e-81 244
MsG0880045975.01.T01 AT4G37650 31.447 318 193 7 28 334 227 530 1.77e-41 152
MsG0880045975.01.T01 AT2G04890 29.043 303 192 6 31 332 132 412 8.32e-35 131
MsG0880045975.01.T01 AT1G50600 28.525 305 198 7 31 332 238 525 1.70e-34 132
MsG0880045975.01.T01 AT1G21450 27.961 304 202 5 31 332 304 592 1.75e-34 133
MsG0880045975.01.T01 AT1G50600 28.525 305 198 7 31 332 309 596 2.53e-34 132
MsG0880045975.01.T01 AT1G50600 28.525 305 198 7 31 332 313 600 2.62e-34 132
MsG0880045975.01.T01 AT5G48150 29.126 309 191 6 31 332 202 489 3.78e-34 131
MsG0880045975.01.T01 AT5G48150 29.126 309 191 6 31 332 202 489 3.78e-34 131
MsG0880045975.01.T01 AT5G48150 29.126 309 191 6 31 332 202 489 3.78e-34 131
MsG0880045975.01.T01 AT5G48150 29.126 309 191 6 31 332 202 489 3.78e-34 131
MsG0880045975.01.T01 AT4G17230 28.955 335 220 7 3 334 207 526 8.36e-32 125
MsG0880045975.01.T01 AT4G17230 28.955 335 220 7 3 334 207 526 8.36e-32 125
MsG0880045975.01.T01 AT3G03450 28.115 313 201 7 23 332 253 544 1.02e-29 119
MsG0880045975.01.T01 AT5G17490 26.647 334 219 7 2 332 205 515 2.63e-29 118
MsG0880045975.01.T01 AT5G52510 25.321 312 213 5 31 332 338 639 2.84e-27 112
MsG0880045975.01.T01 AT3G46600 26.688 311 209 7 28 335 79 373 3.87e-24 102
MsG0880045975.01.T01 AT3G46600 26.774 310 208 7 29 335 256 549 1.97e-23 101
MsG0880045975.01.T01 AT1G14920 25.970 335 221 11 2 332 217 528 2.22e-23 101
MsG0880045975.01.T01 AT3G46600 26.774 310 208 7 29 335 288 581 3.14e-23 100
MsG0880045975.01.T01 AT2G29065 25.664 339 226 9 5 335 310 630 5.85e-23 100
MsG0880045975.01.T01 AT2G01570 25.970 335 221 11 2 332 269 580 6.28e-23 100
MsG0880045975.01.T01 AT3G13840 27.445 317 189 12 26 332 224 509 1.04e-21 96.3
MsG0880045975.01.T01 AT5G59450 25.397 315 208 8 29 335 305 600 5.64e-21 94.4
MsG0880045975.01.T01 AT1G55580 23.473 311 210 7 32 332 152 444 6.58e-21 93.6
MsG0880045975.01.T01 AT1G50420 23.757 362 198 8 29 332 137 478 6.83e-21 93.6
MsG0880045975.01.T01 AT1G63100 24.606 317 199 8 32 332 361 653 2.60e-19 89.4
MsG0880045975.01.T01 AT1G63100 24.606 317 199 8 32 332 336 628 2.81e-19 89.4
MsG0880045975.01.T01 AT1G07530 24.679 312 213 9 29 335 473 767 2.92e-19 89.4
MsG0880045975.01.T01 AT1G66350 22.801 307 209 6 28 332 225 505 7.87e-19 87.8
MsG0880045975.01.T01 AT2G29060 24.561 342 222 11 5 334 339 656 1.45e-18 87.0
MsG0880045975.01.T01 AT2G29060 24.561 342 222 11 5 334 376 693 1.73e-18 87.0
MsG0880045975.01.T01 AT5G66770 27.174 276 176 9 31 301 294 549 6.19e-18 85.1
MsG0880045975.01.T01 AT5G41920 27.445 317 190 13 21 332 118 399 2.08e-17 82.8
MsG0880045975.01.T01 AT1G07520 22.956 318 213 10 29 335 235 531 3.99e-17 82.4
MsG0880045975.01.T01 AT1G07520 22.956 318 213 10 29 335 235 531 3.99e-17 82.4
MsG0880045975.01.T01 AT1G07520 22.956 318 213 10 29 335 400 696 4.34e-17 82.8
MsG0880045975.01.T01 AT1G07520 22.956 318 213 10 29 335 399 695 4.71e-17 82.8
MsG0880045975.01.T01 AT3G54220 25.298 336 211 12 6 334 349 651 5.79e-17 82.4
MsG0880045975.01.T01 AT2G37650 22.078 308 221 8 29 334 412 702 2.90e-15 77.4
MsG0880045975.01.T01 AT2G37650 22.508 311 216 9 29 334 424 714 3.38e-15 77.0
MsG0880045975.01.T01 AT4G08250 25.816 337 207 13 14 332 165 476 7.34e-15 75.5
MsG0880045975.01.T01 AT3G50650 24.920 313 205 9 31 332 248 541 1.78e-12 68.6
MsG0880045975.01.T02 AT3G49950 58.187 342 107 7 17 352 98 409 1.72e-139 402
MsG0880045975.01.T02 AT5G67411 55.947 227 73 4 68 292 1 202 3.14e-78 239
MsG0880045975.01.T02 AT4G37650 29.464 336 194 7 28 352 227 530 2.08e-38 144
MsG0880045975.01.T02 AT1G21450 26.708 322 201 5 31 350 304 592 8.34e-33 129
MsG0880045975.01.T02 AT1G50600 26.935 323 198 7 31 350 238 525 2.36e-32 127
MsG0880045975.01.T02 AT1G50600 26.935 323 198 7 31 350 309 596 2.51e-32 127
MsG0880045975.01.T02 AT1G50600 26.935 323 198 7 31 350 313 600 2.75e-32 127
MsG0880045975.01.T02 AT2G04890 27.414 321 192 6 31 350 132 412 4.67e-32 124
MsG0880045975.01.T02 AT5G48150 27.523 327 191 6 31 350 202 489 1.42e-31 124
MsG0880045975.01.T02 AT5G48150 27.523 327 191 6 31 350 202 489 1.42e-31 124
MsG0880045975.01.T02 AT5G48150 27.523 327 191 6 31 350 202 489 1.42e-31 124
MsG0880045975.01.T02 AT5G48150 27.523 327 191 6 31 350 202 489 1.42e-31 124
MsG0880045975.01.T02 AT4G17230 27.479 353 220 7 3 352 207 526 2.69e-29 118
MsG0880045975.01.T02 AT4G17230 27.479 353 220 7 3 352 207 526 2.69e-29 118
MsG0880045975.01.T02 AT3G03450 26.586 331 201 7 23 350 253 544 3.78e-27 112
MsG0880045975.01.T02 AT5G17490 25.284 352 219 7 2 350 205 515 1.02e-26 111
MsG0880045975.01.T02 AT5G52510 23.939 330 213 5 31 350 338 639 7.83e-25 106
MsG0880045975.01.T02 AT1G50420 24.586 362 213 8 29 350 137 478 5.48e-23 100
MsG0880045975.01.T02 AT3G46600 25.000 328 211 7 28 353 79 373 1.05e-21 95.5
MsG0880045975.01.T02 AT1G14920 24.363 353 222 10 2 350 217 528 2.86e-21 95.1
MsG0880045975.01.T02 AT3G46600 25.228 329 209 7 28 353 255 549 3.75e-21 95.1
MsG0880045975.01.T02 AT3G46600 25.305 328 208 7 29 353 288 581 4.52e-21 94.7
MsG0880045975.01.T02 AT2G01570 24.363 353 222 10 2 350 269 580 1.01e-20 94.0
MsG0880045975.01.T02 AT3G13840 25.970 335 189 12 26 350 224 509 2.19e-19 89.7
MsG0880045975.01.T02 AT5G59450 24.024 333 208 8 29 353 305 600 9.14e-19 88.2
MsG0880045975.01.T02 AT1G07530 22.796 329 216 8 29 353 473 767 7.13e-18 85.5
MsG0880045975.01.T02 AT1G55580 21.884 329 211 7 32 350 152 444 9.02e-18 84.7
MsG0880045975.01.T02 AT1G63100 23.881 335 197 10 32 350 336 628 2.11e-17 84.0
MsG0880045975.01.T02 AT1G63100 23.881 335 197 10 32 350 361 653 2.14e-17 84.0
MsG0880045975.01.T02 AT2G29060 23.333 360 222 11 5 352 339 656 1.20e-16 81.6
MsG0880045975.01.T02 AT2G29060 23.333 360 222 11 5 352 376 693 1.23e-16 81.6
MsG0880045975.01.T02 AT5G66770 26.531 294 173 10 31 319 294 549 1.56e-16 81.3
MsG0880045975.01.T02 AT5G41920 25.970 335 190 13 21 350 118 399 4.15e-15 76.3
MsG0880045975.01.T02 AT3G54220 24.011 354 211 13 6 352 349 651 5.91e-15 76.6
MsG0880045975.01.T02 AT2G37650 21.277 329 216 9 29 352 424 714 2.74e-13 71.6
MsG0880045975.01.T02 AT2G37650 21.277 329 216 9 29 352 412 702 2.95e-13 71.2
MsG0880045975.01.T02 AT4G08250 25.352 355 204 16 14 350 165 476 3.93e-13 70.9

Find 53 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCTTACGACGAATT+AGG 0.234419 8:-67109083 MsG0880045975.01.T02:CDS
TAACACCTTGGCATCGTTTC+GGG 0.237807 8:-67109310 MsG0880045975.01.T02:CDS
CTTTGTTCCCATTTGCCTAT+AGG 0.258304 8:+67108540 None:intergenic
GGTCTGCATTTAATTATCTA+TGG 0.266633 8:-67108695 MsG0880045975.01.T02:CDS
GTTCAACGTTTGGTTTATTC+AGG 0.267502 8:-67108954 MsG0880045975.01.T02:CDS
CATATGAGTTTGAATTTGAT+AGG 0.282770 8:+67108877 None:intergenic
CTTCTTTGATTGAACATTTG+AGG 0.307298 8:-67108977 MsG0880045975.01.T02:CDS
TTCTTTGATTGAACATTTGA+GGG 0.357439 8:-67108976 MsG0880045975.01.T02:CDS
TTAACACCTTGGCATCGTTT+CGG 0.361682 8:-67109311 MsG0880045975.01.T02:CDS
TGAGAATCTGTGTGTATCTA+TGG 0.382234 8:+67109361 None:intergenic
ATGAGCATATTGTGCTTACT+TGG 0.383561 8:-67108410 MsG0880045975.01.T02:CDS
ACAACTTGGTCAGTAGATTA+AGG 0.384086 8:-67108716 MsG0880045975.01.T02:CDS
AGCTGCTAATGCAGCAATTC+TGG 0.384262 8:-67109282 MsG0880045975.01.T02:CDS
ACATTTGAGGGTTCAACGTT+TGG 0.395244 8:-67108964 MsG0880045975.01.T02:CDS
GGTTTATTCAGGTGAAGCTT+TGG 0.398229 8:-67108943 MsG0880045975.01.T02:CDS
TTGTTTCTTAACGCACTTCG+AGG 0.403034 8:-67108792 MsG0880045975.01.T02:CDS
GAATTTGATAGGGTTTCATC+TGG 0.416336 8:+67108888 None:intergenic
GGTATGAAGCAGATATTTGT+TGG 0.435178 8:-67108617 MsG0880045975.01.T02:CDS
GAGTTGAACCTATAGGCAAA+TGG 0.454475 8:-67108548 MsG0880045975.01.T02:CDS
TGATGAACATGCTGCTGGAT+GGG 0.466685 8:-67108448 MsG0880045975.01.T02:CDS
TCGTAAGAAAGATCAAGTTT+AGG 0.472515 8:+67109092 None:intergenic
CATTGAGCTAGCTAACTTCG+TGG 0.473787 8:-67109336 MsG0880045975.01.T02:CDS
GATGAACATGCTGCTGGATG+GGG 0.481456 8:-67108447 MsG0880045975.01.T02:CDS
CGACGAATTAGGTTCTAAGT+TGG 0.484399 8:-67109072 MsG0880045975.01.T02:CDS
GTTGAGAGAGTTGAACCTAT+AGG 0.489058 8:-67108555 MsG0880045975.01.T02:CDS
GCGATAGCATCGATTAATGT+AGG 0.493202 8:+67109194 None:intergenic
GTTTCTTCAAGTTACACAAA+TGG 0.496382 8:-67109005 MsG0880045975.01.T02:CDS
AGTTGAACCTATAGGCAAAT+GGG 0.497812 8:-67108547 MsG0880045975.01.T02:CDS
TGATTTAACTTCAAACAACT+TGG 0.502171 8:-67108730 MsG0880045975.01.T02:CDS
TCTACTAAAATAACAAGTGT+TGG 0.502927 8:+67108762 None:intergenic
ATGCTTGATGAACATGCTGC+TGG 0.503248 8:-67108453 MsG0880045975.01.T02:CDS
CTAAGTTGGTGAATTTCGCT+AGG 0.512357 8:-67109058 MsG0880045975.01.T02:CDS
AATGTGTCTACAGTATCATA+AGG 0.517595 8:+67108669 None:intergenic
ATATGAGTTTGAATTTGATA+GGG 0.529498 8:+67108878 None:intergenic
CATATTGTGCTTACTTGGAA+AGG 0.533221 8:-67108405 MsG0880045975.01.T02:CDS
AAATGTTGAACTAATTAAGC+AGG 0.534799 8:+67108351 None:intergenic
TTGATGAACATGCTGCTGGA+TGG 0.537625 8:-67108449 MsG0880045975.01.T02:CDS
TGATCAAAGGAGCAACTTCG+TGG 0.560682 8:+67109165 None:intergenic
GGATTTGGTGAAGAATGTGT+TGG 0.560912 8:-67108489 MsG0880045975.01.T02:CDS
AGTAGTAGCAGTGGTAGTAG+AGG 0.563825 8:+67108839 None:intergenic
TAAGAAAGATCAAGTTTAGG+TGG 0.577766 8:+67109095 None:intergenic
AGTAGTAATAGTAGTAGCAG+TGG 0.591600 8:+67108830 None:intergenic
GCAATTCTGGAAGCAACAGA+AGG 0.597475 8:-67109269 MsG0880045975.01.T02:CDS
TAGGTCAAGAAACGTGACAT+TGG 0.616837 8:-67109039 MsG0880045975.01.T02:CDS
ACTTCGTGGATTTAACACCT+TGG 0.617259 8:-67109322 MsG0880045975.01.T02:CDS
GAAACAGTGAGTTTGATCAA+AGG 0.618628 8:+67109152 None:intergenic
TGGTGAAGAATGTGTTGGTG+AGG 0.621784 8:-67108484 MsG0880045975.01.T02:CDS
GCGTACCCGAAACGATGCCA+AGG 0.644547 8:+67109305 None:intergenic
GTGGAAGTAAACAAAGGCAA+TGG 0.648634 8:-67108638 MsG0880045975.01.T02:CDS
TGCCTTTGTTTACTTCCACG+TGG 0.684183 8:+67108642 None:intergenic
GAGAATGTGATAGCTCATGA+AGG 0.690740 8:-67108588 MsG0880045975.01.T02:CDS
TGCCACGTGGAAGTAAACAA+AGG 0.696360 8:-67108644 MsG0880045975.01.T02:CDS
GTAGACACATTTCTGCCACG+TGG 0.718193 8:-67108657 MsG0880045975.01.T02:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGAGTTTGAATTTGATA+GGG + Chr8:67108894-67108913 None:intergenic 20.0%
! CATATGAGTTTGAATTTGAT+AGG + Chr8:67108895-67108914 None:intergenic 25.0%
! TCTACTAAAATAACAAGTGT+TGG + Chr8:67109010-67109029 None:intergenic 25.0%
! TTCTTTGATTGAACATTTGA+GGG - Chr8:67108793-67108812 MsG0880045975.01.T02:CDS 25.0%
!! ATGAGAAATGCTAATTTTCA+AGG - Chr8:67109253-67109272 MsG0880045975.01.T02:CDS 25.0%
!! TGAGAAATGCTAATTTTCAA+GGG - Chr8:67109254-67109273 MsG0880045975.01.T02:CDS 25.0%
!! TGATTTAACTTCAAACAACT+TGG - Chr8:67109039-67109058 MsG0880045975.01.T02:CDS 25.0%
AATGTGTCTACAGTATCATA+AGG + Chr8:67109103-67109122 None:intergenic 30.0%
CTTCTTTGATTGAACATTTG+AGG - Chr8:67108792-67108811 MsG0880045975.01.T02:CDS 30.0%
GGTCTGCATTTAATTATCTA+TGG - Chr8:67109074-67109093 MsG0880045975.01.T02:CDS 30.0%
TAAGAAAGATCAAGTTTAGG+TGG + Chr8:67108677-67108696 None:intergenic 30.0%
TCGTAAGAAAGATCAAGTTT+AGG + Chr8:67108680-67108699 None:intergenic 30.0%
! GTTTCTTCAAGTTACACAAA+TGG - Chr8:67108764-67108783 MsG0880045975.01.T02:CDS 30.0%
!! AATGCTAATTTTCAAGGGAT+TGG - Chr8:67109259-67109278 MsG0880045975.01.T02:CDS 30.0%
!! AATTTTCAAGGGATTGGATT+TGG - Chr8:67109265-67109284 MsG0880045975.01.T02:CDS 30.0%
ACAACTTGGTCAGTAGATTA+AGG - Chr8:67109053-67109072 MsG0880045975.01.T02:CDS 35.0%
AGTAGTAATAGTAGTAGCAG+TGG + Chr8:67108942-67108961 None:intergenic 35.0%
AGTTGAACCTATAGGCAAAT+GGG - Chr8:67109222-67109241 MsG0880045975.01.T02:CDS 35.0%
ATGAGCATATTGTGCTTACT+TGG - Chr8:67109359-67109378 MsG0880045975.01.T02:CDS 35.0%
CATATTGTGCTTACTTGGAA+AGG - Chr8:67109364-67109383 MsG0880045975.01.T02:CDS 35.0%
GAAACAGTGAGTTTGATCAA+AGG + Chr8:67108620-67108639 None:intergenic 35.0%
GAATTTGATAGGGTTTCATC+TGG + Chr8:67108884-67108903 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG - Chr8:67108686-67108705 MsG0880045975.01.T02:CDS 35.0%
GGTATGAAGCAGATATTTGT+TGG - Chr8:67109152-67109171 MsG0880045975.01.T02:CDS 35.0%
TGAGAATCTGTGTGTATCTA+TGG + Chr8:67108411-67108430 None:intergenic 35.0%
! GTTCAACGTTTGGTTTATTC+AGG - Chr8:67108815-67108834 MsG0880045975.01.T02:CDS 35.0%
!!! ATGTTGTTTTTGCTTCTGCT+TGG - Chr8:67109392-67109411 MsG0880045975.01.T02:CDS 35.0%
ACATTTGAGGGTTCAACGTT+TGG - Chr8:67108805-67108824 MsG0880045975.01.T02:CDS 40.0%
ACTTCGTGGATTTAACACCT+TGG - Chr8:67108447-67108466 MsG0880045975.01.T02:CDS 40.0%
CTTTGTTCCCATTTGCCTAT+AGG + Chr8:67109232-67109251 None:intergenic 40.0%
GAGAATGTGATAGCTCATGA+AGG - Chr8:67109181-67109200 MsG0880045975.01.T02:CDS 40.0%
GAGTTGAACCTATAGGCAAA+TGG - Chr8:67109221-67109240 MsG0880045975.01.T02:CDS 40.0%
GGTTTATTCAGGTGAAGCTT+TGG - Chr8:67108826-67108845 MsG0880045975.01.T02:CDS 40.0%
GTGGAAGTAAACAAAGGCAA+TGG - Chr8:67109131-67109150 MsG0880045975.01.T02:CDS 40.0%
GTTGAGAGAGTTGAACCTAT+AGG - Chr8:67109214-67109233 MsG0880045975.01.T02:CDS 40.0%
TAGGTCAAGAAACGTGACAT+TGG - Chr8:67108730-67108749 MsG0880045975.01.T02:CDS 40.0%
TTAACACCTTGGCATCGTTT+CGG - Chr8:67108458-67108477 MsG0880045975.01.T02:CDS 40.0%
! TTGTTTCTTAACGCACTTCG+AGG - Chr8:67108977-67108996 MsG0880045975.01.T02:CDS 40.0%
!! CGACGAATTAGGTTCTAAGT+TGG - Chr8:67108697-67108716 MsG0880045975.01.T02:CDS 40.0%
!! CTAAGTTGGTGAATTTCGCT+AGG - Chr8:67108711-67108730 MsG0880045975.01.T02:CDS 40.0%
!! GCGATAGCATCGATTAATGT+AGG + Chr8:67108578-67108597 None:intergenic 40.0%
!! GGATTTGGTGAAGAATGTGT+TGG - Chr8:67109280-67109299 MsG0880045975.01.T02:CDS 40.0%
AGCTGCTAATGCAGCAATTC+TGG - Chr8:67108487-67108506 MsG0880045975.01.T02:CDS 45.0%
AGTAGTAGCAGTGGTAGTAG+AGG + Chr8:67108933-67108952 None:intergenic 45.0%
ATGCTTGATGAACATGCTGC+TGG - Chr8:67109316-67109335 MsG0880045975.01.T02:CDS 45.0%
CATTGAGCTAGCTAACTTCG+TGG - Chr8:67108433-67108452 MsG0880045975.01.T02:CDS 45.0%
TAACACCTTGGCATCGTTTC+GGG - Chr8:67108459-67108478 MsG0880045975.01.T02:CDS 45.0%
TGATCAAAGGAGCAACTTCG+TGG + Chr8:67108607-67108626 None:intergenic 45.0%
TGATGAACATGCTGCTGGAT+GGG - Chr8:67109321-67109340 MsG0880045975.01.T02:CDS 45.0%
TGCCACGTGGAAGTAAACAA+AGG - Chr8:67109125-67109144 MsG0880045975.01.T02:CDS 45.0%
TTGATGAACATGCTGCTGGA+TGG - Chr8:67109320-67109339 MsG0880045975.01.T02:CDS 45.0%
! GCAATTCTGGAAGCAACAGA+AGG - Chr8:67108500-67108519 MsG0880045975.01.T02:CDS 45.0%
! TGCCTTTGTTTACTTCCACG+TGG + Chr8:67109130-67109149 None:intergenic 45.0%
! TGGTGAAGAATGTGTTGGTG+AGG - Chr8:67109285-67109304 MsG0880045975.01.T02:CDS 45.0%
GATGAACATGCTGCTGGATG+GGG - Chr8:67109322-67109341 MsG0880045975.01.T02:CDS 50.0%
GTAGACACATTTCTGCCACG+TGG - Chr8:67109112-67109131 MsG0880045975.01.T02:CDS 50.0%
GCGTACCCGAAACGATGCCA+AGG + Chr8:67108467-67108486 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 67108365 67109426 67108365 ID=MsG0880045975.01;Name=MsG0880045975.01
Chr8 mRNA 67108365 67109426 67108365 ID=MsG0880045975.01.T01;Parent=MsG0880045975.01;Name=MsG0880045975.01.T01;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|0|0|2|0|335
Chr8 exon 67108365 67108827 67108365 ID=MsG0880045975.01.T01:exon:32492;Parent=MsG0880045975.01.T01
Chr8 exon 67108882 67109426 67108882 ID=MsG0880045975.01.T01:exon:32491;Parent=MsG0880045975.01.T01
Chr8 CDS 67108882 67109426 67108882 ID=MsG0880045975.01.T01:cds;Parent=MsG0880045975.01.T01
Chr8 CDS 67108365 67108827 67108365 ID=MsG0880045975.01.T01:cds;Parent=MsG0880045975.01.T01
Chr8 mRNA 67108365 67109426 67108365 ID=MsG0880045975.01.T02;Parent=MsG0880045975.01;Name=MsG0880045975.01.T02;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|353
Chr8 exon 67108365 67109426 67108365 ID=MsG0880045975.01.T02:exon:32493;Parent=MsG0880045975.01.T02
Chr8 CDS 67108365 67109426 67108365 ID=MsG0880045975.01.T02:cds;Parent=MsG0880045975.01.T02
Chr8 mRNA 67108601 67108636 67108601 ID=MsG0880045975.01.T03;Parent=MsG0880045975.01;Name=MsG0880045975.01.T03;_AED=0.49;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|11
Chr8 exon 67108601 67108636 67108601 ID=MsG0880045975.01.T03:exon:32494;Parent=MsG0880045975.01.T03
Chr8 CDS 67108601 67108636 67108601 ID=MsG0880045975.01.T03:cds;Parent=MsG0880045975.01.T03
Gene Sequence

>MsG0880045975.01.T01

ATGCTAGTAGAACAAGTTTATTCAAATGCTCATAACAATCTCACCATAGATACACACAGATTCTCAGTCATTGAGCTAGCTAACTTCGTGGATTTAACACCTTGGCATCGTTTCGGGTACGCAGCTGCTAATGCAGCAATTCTGGAAGCAACAGAAGGTTTCTCAGTGATTCATATAGTTGATTTGAGTTCAACTCATTGCATGCAGATTCCTACATTAATCGATGCTATCGCTAATCGCCACGAAGTTGCTCCTTTGATCAAACTCACTGTTTCTGATCATGCTAACAACAACAGTGATCAACTAATTCCACCTAAACTTGATCTTTCTTACGACGAATTAGGTTCTAAGTTGGTGAATTTCGCTAGGTCAAGAAACGTGACATTGGAATTTAGAGTAGTTTCTTCAAGTTACACAAATGGTTTTGCTTCTTTGATTGAACATTTGAGGGTTCAACGTTTGGTTTATTCAGGTGAAGCTTTGGTTATAAATTGTCACATGATGCTTCATTATATTCCAGATGAAACCCTATCAAATTCAAACTCGTCTCTTAGATCGTTGTTTCTTAACGCACTTCGAGGTTTAGATCCAACACTTGTTATTTTAGTAGATGAAGATGTTGATTTAACTTCAAACAACTTGGTCAGTAGATTAAGGTCTGCATTTAATTATCTATGGATACCTTATGATACTGTAGACACATTTCTGCCACGTGGAAGTAAACAAAGGCAATGGTATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGATAGCTCATGAAGGTGTTCAAAGAGTTGAGAGAGTTGAACCTATAGGCAAATGGGAACAAAGAATGAGAAATGCTAATTTTCAAGGGATTGGATTTGGTGAAGAATGTGTTGGTGAGGTGAAAGCTATGCTTGATGAACATGCTGCTGGATGGGGATTGAAGAAAGAAGATGAGCATATTGTGCTTACTTGGAAAGGACATAATGTTGTTTTTGCTTCTGCTTGGTTACCTGCTTAA

>MsG0880045975.01.T02

ATGCTAGTAGAACAAGTTTATTCAAATGCTCATAACAATCTCACCATAGATACACACAGATTCTCAGTCATTGAGCTAGCTAACTTCGTGGATTTAACACCTTGGCATCGTTTCGGGTACGCAGCTGCTAATGCAGCAATTCTGGAAGCAACAGAAGGTTTCTCAGTGATTCATATAGTTGATTTGAGTTCAACTCATTGCATGCAGATTCCTACATTAATCGATGCTATCGCTAATCGCCACGAAGTTGCTCCTTTGATCAAACTCACTGTTTCTGATCATGCTAACAACAACAGTGATCAACTAATTCCACCTAAACTTGATCTTTCTTACGACGAATTAGGTTCTAAGTTGGTGAATTTCGCTAGGTCAAGAAACGTGACATTGGAATTTAGAGTAGTTTCTTCAAGTTACACAAATGGTTTTGCTTCTTTGATTGAACATTTGAGGGTTCAACGTTTGGTTTATTCAGGTGAAGCTTTGGTTATAAATTGTCACATGATGCTTCATTATATTCCAGATGAAACCCTATCAAATTCAAACTCATATGTTTATGATTCTATGTCCTCTACTACCACTGCTACTACTATTACTACTACGTCTCTTAGATCGTTGTTTCTTAACGCACTTCGAGGTTTAGATCCAACACTTGTTATTTTAGTAGATGAAGATGTTGATTTAACTTCAAACAACTTGGTCAGTAGATTAAGGTCTGCATTTAATTATCTATGGATACCTTATGATACTGTAGACACATTTCTGCCACGTGGAAGTAAACAAAGGCAATGGTATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGATAGCTCATGAAGGTGTTCAAAGAGTTGAGAGAGTTGAACCTATAGGCAAATGGGAACAAAGAATGAGAAATGCTAATTTTCAAGGGATTGGATTTGGTGAAGAATGTGTTGGTGAGGTGAAAGCTATGCTTGATGAACATGCTGCTGGATGGGGATTGAAGAAAGAAGATGAGCATATTGTGCTTACTTGGAAAGGACATAATGTTGTTTTTGCTTCTGCTTGGTTACCTGCTTAA

>MsG0880045975.01.T03

ATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGA
Protein sequence

>MsG0880045975.01.T01

MLVEQVYSNAHNNLTIDTHRFSVIELANFVDLTPWHRFGYAAANAAILEATEGFSVIHIVDLSSTHCMQIPTLIDAIANRHEVAPLIKLTVSDHANNNSDQLIPPKLDLSYDELGSKLVNFARSRNVTLEFRVVSSSYTNGFASLIEHLRVQRLVYSGEALVINCHMMLHYIPDETLSNSNSSLRSLFLNALRGLDPTLVILVDEDVDLTSNNLVSRLRSAFNYLWIPYDTVDTFLPRGSKQRQWYEADICWKIENVIAHEGVQRVERVEPIGKWEQRMRNANFQGIGFGEECVGEVKAMLDEHAAGWGLKKEDEHIVLTWKGHNVVFASAWLPA*

>MsG0880045975.01.T02

MLVEQVYSNAHNNLTIDTHRFSVIELANFVDLTPWHRFGYAAANAAILEATEGFSVIHIVDLSSTHCMQIPTLIDAIANRHEVAPLIKLTVSDHANNNSDQLIPPKLDLSYDELGSKLVNFARSRNVTLEFRVVSSSYTNGFASLIEHLRVQRLVYSGEALVINCHMMLHYIPDETLSNSNSYVYDSMSSTTTATTITTTSLRSLFLNALRGLDPTLVILVDEDVDLTSNNLVSRLRSAFNYLWIPYDTVDTFLPRGSKQRQWYEADICWKIENVIAHEGVQRVERVEPIGKWEQRMRNANFQGIGFGEECVGEVKAMLDEHAAGWGLKKEDEHIVLTWKGHNVVFASAWLPA*

>MsG0880045975.01.T03

MKQIFVGRLRM*