Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046011.01.T01 | XP_003608563.2 | 97.423 | 194 | 5 | 0 | 1 | 194 | 44 | 237 | 1.00E-131 | 382 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046011.01.T01 | A0A0E3D8L9 | 35.714 | 196 | 119 | 4 | 1 | 194 | 41 | 231 | 1.02E-28 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046011.01.T01 | G7JF91 | 97.423 | 194 | 5 | 0 | 1 | 194 | 44 | 237 | 4.78e-132 | 382 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046011.01 | MsG0580024710.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046011.01.T01 | MTR_4g097510 | 97.423 | 194 | 5 | 0 | 1 | 194 | 44 | 237 | 1.21e-135 | 382 |
| MsG0880046011.01.T01 | MTR_3g087860 | 50.761 | 197 | 96 | 1 | 1 | 196 | 51 | 247 | 3.93e-68 | 211 |
| MsG0880046011.01.T01 | MTR_3g087850 | 49.000 | 200 | 95 | 2 | 1 | 196 | 66 | 262 | 1.34e-62 | 197 |
| MsG0880046011.01.T01 | MTR_4g052350 | 31.646 | 158 | 104 | 3 | 13 | 167 | 68 | 224 | 3.02e-15 | 73.2 |
| MsG0880046011.01.T01 | MTR_4g052340 | 30.128 | 156 | 105 | 3 | 13 | 165 | 68 | 222 | 2.75e-13 | 67.4 |
| MsG0880046011.01.T01 | MTR_3g085740 | 32.308 | 130 | 88 | 0 | 16 | 145 | 135 | 264 | 1.83e-12 | 65.9 |
| MsG0880046011.01.T01 | MTR_4g052300 | 30.469 | 128 | 87 | 1 | 13 | 138 | 62 | 189 | 3.00e-12 | 64.7 |
| MsG0880046011.01.T01 | MTR_1563s0010 | 27.811 | 169 | 112 | 3 | 9 | 168 | 166 | 333 | 4.06e-12 | 65.1 |
| MsG0880046011.01.T01 | MTR_7g105590 | 28.148 | 135 | 95 | 1 | 9 | 141 | 58 | 192 | 1.91e-11 | 61.2 |
| MsG0880046011.01.T01 | MTR_7g083870 | 29.032 | 186 | 122 | 4 | 5 | 185 | 80 | 260 | 2.95e-11 | 62.0 |
| MsG0880046011.01.T01 | MTR_3g113870 | 27.815 | 151 | 107 | 1 | 5 | 153 | 66 | 216 | 4.51e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046011.01.T01 | AT5G10050 | 47.236 | 199 | 104 | 1 | 1 | 198 | 45 | 243 | 4.14e-61 | 192 |
| MsG0880046011.01.T01 | AT5G65205 | 46.734 | 199 | 105 | 1 | 1 | 198 | 46 | 244 | 2.65e-60 | 191 |
| MsG0880046011.01.T01 | AT3G03330 | 29.577 | 142 | 96 | 3 | 6 | 143 | 92 | 233 | 4.69e-14 | 70.5 |
| MsG0880046011.01.T01 | AT3G03350 | 35.052 | 97 | 63 | 0 | 47 | 143 | 170 | 266 | 3.66e-13 | 67.4 |
| MsG0880046011.01.T01 | AT3G47360 | 28.743 | 167 | 112 | 4 | 9 | 174 | 98 | 258 | 4.23e-13 | 67.4 |
| MsG0880046011.01.T01 | AT3G03350 | 37.209 | 86 | 54 | 0 | 58 | 143 | 267 | 352 | 9.29e-13 | 67.0 |
| MsG0880046011.01.T01 | AT1G10310 | 33.103 | 145 | 95 | 2 | 2 | 145 | 59 | 202 | 4.01e-12 | 63.9 |
| MsG0880046011.01.T01 | AT3G47350 | 27.500 | 160 | 109 | 4 | 16 | 174 | 104 | 257 | 1.21e-11 | 63.2 |
| MsG0880046011.01.T01 | AT3G47350 | 27.500 | 160 | 109 | 4 | 16 | 174 | 104 | 257 | 1.56e-11 | 63.2 |
| MsG0880046011.01.T01 | AT3G26760 | 27.206 | 136 | 96 | 1 | 13 | 145 | 89 | 224 | 8.95e-11 | 60.8 |
Find 45 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGTTTGCCTTGCTTTCT+TGG | 0.123954 | 8:+67610178 | MsG0880046011.01.T01:CDS |
| TGCTTTCTTGGTCTCCTCTT+TGG | 0.145587 | 8:+67610190 | MsG0880046011.01.T01:CDS |
| GACATGTTGTTAATAAAATT+TGG | 0.158787 | 8:+67610114 | MsG0880046011.01.T01:CDS |
| GGATCCATTAGATCAAATTT+AGG | 0.199600 | 8:+67609966 | MsG0880046011.01.T01:CDS |
| AAATTTAGGGAAAGCTAATT+TGG | 0.220060 | 8:+67609980 | MsG0880046011.01.T01:CDS |
| TTTCCCTAAATTTGATCTAA+TGG | 0.284776 | 8:-67609970 | None:intergenic |
| GCTTTCTTGGTCTCCTCTTT+GGG | 0.287708 | 8:+67610191 | MsG0880046011.01.T01:CDS |
| ATTTGGGATCAATGTTGTTT+TGG | 0.303734 | 8:+67609935 | MsG0880046011.01.T01:CDS |
| GATCCATTAGATCAAATTTA+GGG | 0.319378 | 8:+67609967 | MsG0880046011.01.T01:CDS |
| CGGTTGGAATTGAAGCCATT+TGG | 0.328978 | 8:+67609918 | MsG0880046011.01.T01:CDS |
| AGAAATTAAGTGATTATGAT+TGG | 0.362854 | 8:+67610003 | MsG0880046011.01.T01:CDS |
| CTAGTTAATAACGCCGGAAT+AGG | 0.382892 | 8:+67609669 | MsG0880046011.01.T01:CDS |
| TTATGATTGGAAGCTTTATA+AGG | 0.384000 | 8:+67610016 | MsG0880046011.01.T01:CDS |
| GGTTGGAATTGAAGCCATTT+GGG | 0.384724 | 8:+67609919 | MsG0880046011.01.T01:CDS |
| AATAGTGTCCAATGGTAATT+CGG | 0.407590 | 8:-67609709 | None:intergenic |
| TCCAATGGTAATTCGGCTAA+TGG | 0.412469 | 8:-67609702 | None:intergenic |
| TGGACCCGTGCTACCTATTC+CGG | 0.423024 | 8:-67609682 | None:intergenic |
| GTTAATGTTGGAAGTGTAGT+TGG | 0.436194 | 8:+67609822 | MsG0880046011.01.T01:CDS |
| ATGCAATGTCAAATAGTTTG+CGG | 0.453327 | 8:+67609898 | MsG0880046011.01.T01:CDS |
| CACCATTCTTAGTTGTCCCA+AGG | 0.475460 | 8:-67609754 | None:intergenic |
| AAAACAACATTGATCCCAAA+TGG | 0.482655 | 8:-67609933 | None:intergenic |
| ACATGGGAAATTAACACCTT+GGG | 0.485078 | 8:+67609738 | MsG0880046011.01.T01:CDS |
| TCCATTAGCCGAATTACCAT+TGG | 0.499049 | 8:+67609701 | MsG0880046011.01.T01:CDS |
| AACATGGGAAATTAACACCT+TGG | 0.499992 | 8:+67609737 | MsG0880046011.01.T01:CDS |
| AATATTTCAACACCATGGGC+AGG | 0.510602 | 8:+67609846 | MsG0880046011.01.T01:CDS |
| AAATTTGATCTAATGGATCC+AGG | 0.519907 | 8:-67609963 | None:intergenic |
| CACCTTGGGACAACTAAGAA+TGG | 0.530987 | 8:+67609752 | MsG0880046011.01.T01:CDS |
| AGTGGAACCATCGTTAATGT+TGG | 0.537447 | 8:+67609810 | MsG0880046011.01.T01:CDS |
| AAGAGGAGACCAAGAAAGCA+AGG | 0.546315 | 8:-67610187 | None:intergenic |
| AACGCCGGAATAGGTAGCAC+GGG | 0.564856 | 8:+67609678 | MsG0880046011.01.T01:CDS |
| TGGCAATATTTCAACACCAT+GGG | 0.568855 | 8:+67609842 | MsG0880046011.01.T01:CDS |
| AATGTCAAATAGTTTGCGGT+TGG | 0.584405 | 8:+67609902 | MsG0880046011.01.T01:CDS |
| TTCTCAAGGTGGAAAAGCAA+CGG | 0.584952 | 8:+67610073 | MsG0880046011.01.T01:CDS |
| AACTTGTCTCTAACCCAAAG+AGG | 0.585378 | 8:-67610204 | None:intergenic |
| TTGGCAATATTTCAACACCA+TGG | 0.595003 | 8:+67609841 | MsG0880046011.01.T01:CDS |
| TACACTTCCAACATTAACGA+TGG | 0.611889 | 8:-67609817 | None:intergenic |
| TCAAAGCGCTATTTCAGCCG+TGG | 0.621512 | 8:+67609611 | MsG0880046011.01.T01:CDS |
| TAACGCCGGAATAGGTAGCA+CGG | 0.621530 | 8:+67609677 | MsG0880046011.01.T01:CDS |
| GAGAGAGCAAGAGCTTCTCA+AGG | 0.630585 | 8:+67610059 | MsG0880046011.01.T01:CDS |
| ATATTTCAACACCATGGGCA+GGG | 0.632029 | 8:+67609847 | MsG0880046011.01.T01:CDS |
| GCAACAAGTTGTGCCTCACA+TGG | 0.637422 | 8:+67609776 | MsG0880046011.01.T01:CDS |
| GATATTCTAGTTAATAACGC+CGG | 0.641346 | 8:+67609663 | MsG0880046011.01.T01:CDS |
| GCACAATATGACCCTGCCCA+TGG | 0.641675 | 8:-67609858 | None:intergenic |
| AGAGCAAGAGCTTCTCAAGG+TGG | 0.645766 | 8:+67610062 | MsG0880046011.01.T01:CDS |
| GACACTATTATATCAAAACA+CGG | 0.684446 | 8:+67609633 | MsG0880046011.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAATTAAGTGATTATGAT+TGG | + | Chr8:67610003-67610022 | MsG0880046011.01.T01:CDS | 20.0% |
| !! | GACATGTTGTTAATAAAATT+TGG | + | Chr8:67610114-67610133 | MsG0880046011.01.T01:CDS | 20.0% |
| ! | AAATTTAGGGAAAGCTAATT+TGG | + | Chr8:67609980-67609999 | MsG0880046011.01.T01:CDS | 25.0% |
| ! | GACACTATTATATCAAAACA+CGG | + | Chr8:67609633-67609652 | MsG0880046011.01.T01:CDS | 25.0% |
| ! | GATCCATTAGATCAAATTTA+GGG | + | Chr8:67609967-67609986 | MsG0880046011.01.T01:CDS | 25.0% |
| ! | TTTCCCTAAATTTGATCTAA+TGG | - | Chr8:67609973-67609992 | None:intergenic | 25.0% |
| !! | TTATGATTGGAAGCTTTATA+AGG | + | Chr8:67610016-67610035 | MsG0880046011.01.T01:CDS | 25.0% |
| !! | TTTTGATATAATAGTGTCCA+CGG | - | Chr8:67609631-67609650 | None:intergenic | 25.0% |
| !!! | GAAGCTTTATAAGGATTTTA+AGG | + | Chr8:67610025-67610044 | MsG0880046011.01.T01:CDS | 25.0% |
| AAAACAACATTGATCCCAAA+TGG | - | Chr8:67609936-67609955 | None:intergenic | 30.0% | |
| AATAGTGTCCAATGGTAATT+CGG | - | Chr8:67609712-67609731 | None:intergenic | 30.0% | |
| ATGCAATGTCAAATAGTTTG+CGG | + | Chr8:67609898-67609917 | MsG0880046011.01.T01:CDS | 30.0% | |
| GGACACTATTAGAAAAACAT+GGG | + | Chr8:67609722-67609741 | MsG0880046011.01.T01:CDS | 30.0% | |
| GGATCCATTAGATCAAATTT+AGG | + | Chr8:67609966-67609985 | MsG0880046011.01.T01:CDS | 30.0% | |
| TGGACACTATTAGAAAAACA+TGG | + | Chr8:67609721-67609740 | MsG0880046011.01.T01:CDS | 30.0% | |
| ! | AAATTTGATCTAATGGATCC+AGG | - | Chr8:67609966-67609985 | None:intergenic | 30.0% |
| ! | GATATTCTAGTTAATAACGC+CGG | + | Chr8:67609663-67609682 | MsG0880046011.01.T01:CDS | 30.0% |
| ! | GTTTTTCTAATAGTGTCCAA+TGG | - | Chr8:67609720-67609739 | None:intergenic | 30.0% |
| !! | ATTTGGGATCAATGTTGTTT+TGG | + | Chr8:67609935-67609954 | MsG0880046011.01.T01:CDS | 30.0% |
| !! | GGATTTTAAGGAAGCTATAT+CGG | + | Chr8:67610037-67610056 | MsG0880046011.01.T01:CDS | 30.0% |
| AACATGGGAAATTAACACCT+TGG | + | Chr8:67609737-67609756 | MsG0880046011.01.T01:CDS | 35.0% | |
| ACATGGGAAATTAACACCTT+GGG | + | Chr8:67609738-67609757 | MsG0880046011.01.T01:CDS | 35.0% | |
| ACCAAAAACATTTGCTTAGG+TGG | - | Chr8:67610148-67610167 | None:intergenic | 35.0% | |
| GTGACCAAAAACATTTGCTT+AGG | - | Chr8:67610151-67610170 | None:intergenic | 35.0% | |
| TACACTTCCAACATTAACGA+TGG | - | Chr8:67609820-67609839 | None:intergenic | 35.0% | |
| TGGCAATATTTCAACACCAT+GGG | + | Chr8:67609842-67609861 | MsG0880046011.01.T01:CDS | 35.0% | |
| TTGGCAATATTTCAACACCA+TGG | + | Chr8:67609841-67609860 | MsG0880046011.01.T01:CDS | 35.0% | |
| ! | AATGTCAAATAGTTTGCGGT+TGG | + | Chr8:67609902-67609921 | MsG0880046011.01.T01:CDS | 35.0% |
| ! | ACCACCTAAGCAAATGTTTT+TGG | + | Chr8:67610144-67610163 | MsG0880046011.01.T01:CDS | 35.0% |
| ! | GTTAATGTTGGAAGTGTAGT+TGG | + | Chr8:67609822-67609841 | MsG0880046011.01.T01:CDS | 35.0% |
| !!! | AATGTTGTTTTGGTTATGCC+TGG | + | Chr8:67609945-67609964 | MsG0880046011.01.T01:CDS | 35.0% |
| !!! | AATGTTTTTGGTCACTTGAC+AGG | + | Chr8:67610156-67610175 | MsG0880046011.01.T01:CDS | 35.0% |
| AATATTTCAACACCATGGGC+AGG | + | Chr8:67609846-67609865 | MsG0880046011.01.T01:CDS | 40.0% | |
| AGTGGAACCATCGTTAATGT+TGG | + | Chr8:67609810-67609829 | MsG0880046011.01.T01:CDS | 40.0% | |
| ATATTTCAACACCATGGGCA+GGG | + | Chr8:67609847-67609866 | MsG0880046011.01.T01:CDS | 40.0% | |
| CTAGTTAATAACGCCGGAAT+AGG | + | Chr8:67609669-67609688 | MsG0880046011.01.T01:CDS | 40.0% | |
| GGTTGGAATTGAAGCCATTT+GGG | + | Chr8:67609919-67609938 | MsG0880046011.01.T01:CDS | 40.0% | |
| GTTTGTTTGCCTTGCTTTCT+TGG | + | Chr8:67610178-67610197 | MsG0880046011.01.T01:CDS | 40.0% | |
| TCCAATGGTAATTCGGCTAA+TGG | - | Chr8:67609705-67609724 | None:intergenic | 40.0% | |
| TCCATTAGCCGAATTACCAT+TGG | + | Chr8:67609701-67609720 | MsG0880046011.01.T01:CDS | 40.0% | |
| TTCTCAAGGTGGAAAAGCAA+CGG | + | Chr8:67610073-67610092 | MsG0880046011.01.T01:CDS | 40.0% | |
| !! | CACATGGCTCTGAAAAAAAG+TGG | + | Chr8:67609792-67609811 | MsG0880046011.01.T01:CDS | 40.0% |
| !!! | CTTTTTTTCAGAGCCATGTG+AGG | - | Chr8:67609792-67609811 | None:intergenic | 40.0% |
| AAGAGGAGACCAAGAAAGCA+AGG | - | Chr8:67610190-67610209 | None:intergenic | 45.0% | |
| CACCATTCTTAGTTGTCCCA+AGG | - | Chr8:67609757-67609776 | None:intergenic | 45.0% | |
| CACCTTGGGACAACTAAGAA+TGG | + | Chr8:67609752-67609771 | MsG0880046011.01.T01:CDS | 45.0% | |
| CGGTTGGAATTGAAGCCATT+TGG | + | Chr8:67609918-67609937 | MsG0880046011.01.T01:CDS | 45.0% | |
| GCTTTCTTGGTCTCCTCTTT+GGG | + | Chr8:67610191-67610210 | MsG0880046011.01.T01:CDS | 45.0% | |
| TGCTTTCTTGGTCTCCTCTT+TGG | + | Chr8:67610190-67610209 | MsG0880046011.01.T01:CDS | 45.0% | |
| AGAGCAAGAGCTTCTCAAGG+TGG | + | Chr8:67610062-67610081 | MsG0880046011.01.T01:CDS | 50.0% | |
| GAGAGAGCAAGAGCTTCTCA+AGG | + | Chr8:67610059-67610078 | MsG0880046011.01.T01:CDS | 50.0% | |
| GCAACAAGTTGTGCCTCACA+TGG | + | Chr8:67609776-67609795 | MsG0880046011.01.T01:CDS | 50.0% | |
| TAACGCCGGAATAGGTAGCA+CGG | + | Chr8:67609677-67609696 | MsG0880046011.01.T01:CDS | 50.0% | |
| ! | TCAAAGCGCTATTTCAGCCG+TGG | + | Chr8:67609611-67609630 | MsG0880046011.01.T01:CDS | 50.0% |
| AACGCCGGAATAGGTAGCAC+GGG | + | Chr8:67609678-67609697 | MsG0880046011.01.T01:CDS | 55.0% | |
| GCACAATATGACCCTGCCCA+TGG | - | Chr8:67609861-67609880 | None:intergenic | 55.0% | |
| TGGACCCGTGCTACCTATTC+CGG | - | Chr8:67609685-67609704 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 67609552 | 67610217 | 67609552 | ID=MsG0880046011.01;Name=MsG0880046011.01 |
| Chr8 | mRNA | 67609552 | 67610217 | 67609552 | ID=MsG0880046011.01.T01;Parent=MsG0880046011.01;Name=MsG0880046011.01.T01;_AED=0.11;_eAED=0.11;_QI=0|-1|0|1|-1|1|1|0|221 |
| Chr8 | exon | 67609552 | 67610217 | 67609552 | ID=MsG0880046011.01.T01:exon:611;Parent=MsG0880046011.01.T01 |
| Chr8 | CDS | 67609552 | 67610217 | 67609552 | ID=MsG0880046011.01.T01:cds;Parent=MsG0880046011.01.T01 |
| Gene Sequence |
| Protein sequence |
