AlfalfaGEDB | MsG0580024710.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024710.01

KEGG: -
GO: GO:0005515
PFAM: PF12799

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024710.01.T01	XP_003611538.1	98.901	273	2	1	1	273	1	272	0	537

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024710.01.T01	Q9M2Y3	70.238	252	72	2	24	273	24	274	2.19E-124	358

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024710.01.T01	G7K170	98.901	273	2	1	1	273	1	272	0.0	537

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000076.01	MsG0580024710.01	0.800081	1.703352e-48	7.859780e-46
MsG0480019588.01	MsG0580024710.01	0.805016	1.636156e-49	8.562678e-47

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PPI

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Gene1	Gene2	Type
MsG0380017978.01	MsG0580024710.01	PPI
MsG0380017978.01	MsG0580024710.01	PPI
MsG0580024710.01	MsG0180002505.01	PPI
MsG0580024710.01	MsG0780041253.01	PPI
MsG0880046011.01	MsG0580024710.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024710.01.T01	MTR_5g015050	98.901	273	2	1	1	273	1	272	0.0	537
MsG0580024710.01.T01	MTR_7g080810	35.841	226	131	5	27	248	31	246	7.53e-31	121
MsG0580024710.01.T01	MTR_4g092530	37.356	174	101	4	18	187	2	171	1.22e-28	114
MsG0580024710.01.T01	MTR_6g009370	31.884	276	160	11	10	273	11	270	9.32e-28	112
MsG0580024710.01.T01	MTR_8g010180	37.363	182	101	5	10	186	20	193	2.40e-26	108
MsG0580024710.01.T01	MTR_0070s0040	38.500	200	112	5	14	209	19	211	5.13e-26	102
MsG0580024710.01.T01	MTR_8g469670	41.081	185	98	5	14	194	19	196	5.78e-26	102
MsG0580024710.01.T01	MTR_8g470970	36.667	210	115	6	14	219	18	213	1.54e-25	103
MsG0580024710.01.T01	MTR_4g029710	43.195	169	88	3	41	207	28	190	1.81e-25	105
MsG0580024710.01.T01	MTR_4g029710	32.487	197	118	5	75	263	542	731	5.43e-15	75.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024710.01.T01	AT3G49750	70.238	252	72	2	24	273	24	274	2.23e-125	358
MsG0580024710.01.T01	AT5G65830	68.651	252	78	1	22	273	29	279	1.31e-118	341
MsG0580024710.01.T01	AT3G49750	73.125	160	43	0	24	183	24	183	3.72e-78	235
MsG0580024710.01.T01	AT3G28450	38.043	276	139	11	10	269	14	273	1.42e-35	134
MsG0580024710.01.T01	AT1G69990	35.798	257	146	7	7	258	1	243	4.25e-32	124
MsG0580024710.01.T01	AT5G48380	31.076	251	151	6	6	243	2	243	4.62e-29	116
MsG0580024710.01.T01	AT1G27190	37.450	251	132	8	7	243	1	240	1.50e-27	111
MsG0580024710.01.T01	AT3G25560	38.750	160	92	2	31	190	45	198	1.28e-24	103
MsG0580024710.01.T01	AT3G25560	38.750	160	92	2	31	190	45	198	1.39e-24	103
MsG0580024710.01.T01	AT5G62710	31.395	258	139	7	28	259	27	272	7.39e-24	101

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Find 58 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACTTGCATTAAATC+TGG	0.257219	5:+8630454	None:intergenic
AAACCCTTAGTTCTTAACGG+CGG	0.716298	5:+8630398	None:intergenic
AAAGAACTTGCATTAAATCT+GGG	0.488417	5:+8630455	None:intergenic
AAAGGAATACTCTGCAACAA+TGG	0.535917	5:-8630851	MsG0580024710.01.T01:CDS
AACCCTAATTGCTGAGGAAT+TGG	0.289766	5:+8630569	None:intergenic
AACTAAGGGTTTATCTATTA+TGG	0.223095	5:-8630387	MsG0580024710.01.T01:CDS
ACAAGACCCAAACAAATCCT+TGG	0.497405	5:-8630930	MsG0580024710.01.T01:CDS
ACCAAAGATTGTAATTCTGG+AGG	0.565848	5:+8630707	None:intergenic
ACCAATAACCCTAATTGCTG+AGG	0.601234	5:+8630563	None:intergenic
AGAAGTTAGTATTAATGTTT+TGG	0.271077	5:-8631024	MsG0580024710.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAACAAATCCTTGGAAAAT+TGG	-	Chr5:8630371-8630390	MsG0580024710.01.T01:CDS	30.0%
TTCACCAAAGATTGTAATTC+TGG	+	Chr5:8630592-8630611	None:intergenic	30.0%
TTTCATGATAATCTGCTTAC+TGG	-	Chr5:8630700-8630719	MsG0580024710.01.T01:CDS	30.0%
AAAGGAATACTCTGCAACAA+TGG	-	Chr5:8630442-8630461	MsG0580024710.01.T01:CDS	35.0%
ACCAAAGATTGTAATTCTGG+AGG	+	Chr5:8630589-8630608	None:intergenic	35.0%
CCAAAGATTGTAATTCTGGA+GGG	+	Chr5:8630588-8630607	None:intergenic	35.0%
CTTTCATCAAATCGTCTTGA+AGG	-	Chr5:8630628-8630647	MsG0580024710.01.T01:CDS	35.0%
GAAGGGAATAAGGATCTTTA+CGG	-	Chr5:8630862-8630881	MsG0580024710.01.T01:CDS	35.0%
GCATTAAATCTGGGTAAGTT+AGG	+	Chr5:8630832-8630851	None:intergenic	35.0%
GGAAAGTTGCATTAGAAGAA+GGG	-	Chr5:8631016-8631035	MsG0580024710.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	8630247	8631068	8630247	ID=MsG0580024710.01;Name=MsG0580024710.01
Chr5	mRNA	8630247	8631068	8630247	ID=MsG0580024710.01.T01;Parent=MsG0580024710.01;Name=MsG0580024710.01.T01;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|273
Chr5	exon	8630247	8631068	8630247	ID=MsG0580024710.01.T01:exon:19480;Parent=MsG0580024710.01.T01
Chr5	CDS	8630247	8631068	8630247	ID=MsG0580024710.01.T01:cds;Parent=MsG0580024710.01.T01

Gene Sequence

>MsG0580024710.01.T01

ATGGATGTAGCATGTTTAATGAAGAAGTTAGTATTAATGTTTTGGATTACATTAATCACATCAATTAAATCAGATCCAAGCGATGAAGCATGTTTAACCCATTTAAACAAATCACTACAAGACCCAAACAAATCCTTGGAAAATTGGAACGAAGCCACTTTCTCAAAACCCTGCAATGAATCAACCTCAACTCTCAAAGGAATACTCTGCAACAATGGCAGAATCTACAAACTCTCGCTCAACAATCTCTCTCTCCGTGGAACAATCTCTCCCTTTCTCTCCAACTGTACCAATCTTCAAACCCTAGACCTCTCTTCCAACTTCATCACAGGACCAATCCCTCCAGAATTACAATCTTTGGTGAATCTTGCTGTGTTGAATCTTTCATCAAATCGTCTTGAAGGTGAGATTCCACCTCAACTCACATTGTGTGCATATCTTAACATAATCGATTTTCATGATAATCTGCTTACTGGTCCAATTCCTCAGCAATTAGGGTTATTGGTTCGCCTCTCGGCTTTTGATGTATCGAATAATAGACTCTCCGGTCCGATTCCAGCGTCGTTATCGAACCGGAGTAGTCCTAACTTACCCAGATTTAATGCAAGTTCTTTTGAAGGGAATAAGGATCTTTACGGTTATCCGTTACCGCCGTTAAGAACTAAGGGTTTATCTATTATGGCTATTGTTTTAATTGGAGTAGGAAGTGGATTAGCGAGTCTTATTATAAGTTTTACTGGGGTTTGTATTTGGTTAAAAGTTACTGATCGGAAAGTTGCATTAGAAGAAGGGAAAGTTAGTCATCTTATGCCTGATTATTGA

Protein sequence

>MsG0580024710.01.T01

MDVACLMKKLVLMFWITLITSIKSDPSDEACLTHLNKSLQDPNKSLENWNEATFSKPCNESTSTLKGILCNNGRIYKLSLNNLSLRGTISPFLSNCTNLQTLDLSSNFITGPIPPELQSLVNLAVLNLSSNRLEGEIPPQLTLCAYLNIIDFHDNLLTGPIPQQLGLLVRLSAFDVSNNRLSGPIPASLSNRSSPNLPRFNASSFEGNKDLYGYPLPPLRTKGLSIMAIVLIGVGSGLASLIISFTGVCIWLKVTDRKVALEEGKVSHLMPDY*