AlfalfaGEDB | MsG0880046136.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046136.01

KEGG: -
GO: GO:0016491 GO:0055114
PFAM: PF03171 PF14226

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046136.01.T01	KAB2622726.1	55.41	610	213	5	3	553	2	611	0	654

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046136.01.T01	C8VK14	32.226	301	177	9	270	552	30	321	7.37E-41	154

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046136.01.T01	A0A5N5H486	55.410	610	213	5	3	553	2	611	0.0	654

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0880046136.01	MsG0280009892.01	PPI
MsG0880046136.01	MsG0480023809.01	PPI
MsG0880046136.01	MsG0580029202.01	PPI
MsG0880046136.01	MsG0280009893.01	PPI
MsG0880046136.01	MsG0280009917.01	PPI
MsG0880046136.01	MsG0280009915.01	PPI
MsG0880046136.01	MsG0180001259.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046136.01.T01	MTR_4g100600	83.959	293	46	1	265	557	23	314	1.17e-179	509
MsG0880046136.01.T01	MTR_4g100600	86.100	259	35	1	1	258	1	259	1.20e-153	443
MsG0880046136.01.T01	MTR_4g100590	76.849	311	67	2	247	557	4	309	2.25e-173	493
MsG0880046136.01.T01	MTR_4g100590	84.716	229	30	2	1	229	1	224	1.01e-133	392
MsG0880046136.01.T01	MTR_4g100590	80.597	268	47	2	291	558	1	263	7.63e-157	449
MsG0880046136.01.T01	MTR_4g100590	86.813	182	19	2	48	229	1	177	1.01e-104	316
MsG0880046136.01.T01	MTR_4g100600	94.760	229	12	0	1	229	1	229	2.42e-155	443
MsG0880046136.01.T01	MTR_4g100600	79.724	217	44	0	256	472	13	229	1.49e-121	357
MsG0880046136.01.T01	MTR_8g102600	49.129	287	137	4	270	548	30	315	2.89e-93	288
MsG0880046136.01.T01	MTR_8g102600	45.852	229	115	4	9	229	12	239	4.38e-60	202

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046136.01.T01	AT4G16765	62.602	246	90	2	310	553	1	246	7.29e-108	323
MsG0880046136.01.T01	AT4G16765	53.125	192	87	3	67	255	1	192	3.19e-59	197
MsG0880046136.01.T01	AT4G16765	62.602	246	90	2	310	553	1	246	7.29e-108	323
MsG0880046136.01.T01	AT4G16765	53.125	192	87	3	67	255	1	192	3.19e-59	197
MsG0880046136.01.T01	AT4G16765	62.602	246	90	2	310	553	1	246	7.29e-108	323
MsG0880046136.01.T01	AT4G16765	53.125	192	87	3	67	255	1	192	3.19e-59	197
MsG0880046136.01.T01	AT4G16765	62.996	227	82	2	310	534	1	227	2.82e-98	298
MsG0880046136.01.T01	AT4G16765	53.125	192	87	3	67	255	1	192	3.39e-59	197
MsG0880046136.01.T01	AT3G46500	42.908	282	156	3	270	548	35	314	1.48e-79	253
MsG0880046136.01.T01	AT3G46500	39.749	239	139	3	8	243	16	252	1.26e-52	182

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Find 142 sgRNAs with CRISPR-Local

Find 555 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTAGGAGATGGCAGCAA+CGG	0.604932	8:-69634035	MsG0880046136.01.T01:exon
AAATTCGATCAGGTGAGGAG+AGG	0.672580	8:+69633987	None:intergenic
AAGATGAGAACCTGCAGTCC+CGG	0.519271	8:+69632256	None:intergenic
AAGATGAGAACCTGCAGTCC+TGG	0.436401	8:+69626362	None:intergenic
AAGTAGTCCCCACTACGTAT+TGG	0.507713	8:+69624119	None:intergenic
AATAGGCGAAGAAAAGCTTC+TGG	0.538698	8:+69632490	None:intergenic
AATTGGAAAAGAACGTTATT+CGG	0.364318	8:-69625533	MsG0880046136.01.T01:intron
ACAAACCTGGATAGTGCAAA+AGG	0.520375	8:+69626663	None:intergenic
ACACTTGTGGTATCTGTTAA+AGG	0.231716	8:+69627050	None:intergenic
ACACTTGTGGTATCTGTTAA+AGG	0.231716	8:+69633277	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACTTCTGGTATGCTTA+AGG	+	Chr8:69631488-69631507	None:intergenic	30.0%
AAATAAAGCAAACAAGGAAG+TGG	+	Chr8:69632679-69632698	None:intergenic	30.0%
AAATGTTTCCACAAAGTAGA+CGG	+	Chr8:69625183-69625202	None:intergenic	30.0%
AACCGAACAGATAAAAATAG+AGG	+	Chr8:69630335-69630354	None:intergenic	30.0%
AATTGGTCCCTAAATCTATA+AGG	-	Chr8:69629620-69629639	MsG0880046136.01.T01:intron	30.0%
ACAACATTAAAGGTTGGAAT+GGG	-	Chr8:69633625-69633644	MsG0880046136.01.T01:CDS	30.0%
ACTCAAAGAAGACAATACAT+TGG	-	Chr8:69628270-69628289	MsG0880046136.01.T01:intron	30.0%
AGAAACGTTAACTAATGACA+TGG	-	Chr8:69627143-69627162	MsG0880046136.01.T01:intron	30.0%
AGGAAATTTATACCATCACA+AGG	+	Chr8:69631168-69631187	None:intergenic	30.0%
AGGTATGAGATTTATAACCT+AGG	+	Chr8:69632152-69632171	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	69624070	69634128	69624070	ID=MsG0880046136.01;Name=MsG0880046136.01
Chr8	mRNA	69624070	69634128	69624070	ID=MsG0880046136.01.T01;Parent=MsG0880046136.01;Name=MsG0880046136.01.T01;_AED=0.44;_eAED=0.46;_QI=81\|0.33\|0.42\|1\|1\|1\|19\|0\|558
Chr8	exon	69633964	69634128	69633964	ID=MsG0880046136.01.T01:exon:16945;Parent=MsG0880046136.01.T01
Chr8	exon	69633574	69633766	69633574	ID=MsG0880046136.01.T01:exon:16946;Parent=MsG0880046136.01.T01
Chr8	exon	69633251	69633328	69633251	ID=MsG0880046136.01.T01:exon:16947;Parent=MsG0880046136.01.T01
Chr8	exon	69633071	69633122	69633071	ID=MsG0880046136.01.T01:exon:16948;Parent=MsG0880046136.01.T01
Chr8	exon	69632479	69632600	69632479	ID=MsG0880046136.01.T01:exon:16949;Parent=MsG0880046136.01.T01
Chr8	exon	69632267	69632364	69632267	ID=MsG0880046136.01.T01:exon:16950;Parent=MsG0880046136.01.T01
Chr8	exon	69632101	69632159	69632101	ID=MsG0880046136.01.T01:exon:16951;Parent=MsG0880046136.01.T01
Chr8	exon	69631596	69631675	69631596	ID=MsG0880046136.01.T01:exon:16952;Parent=MsG0880046136.01.T01
Chr8	exon	69630156	69630202	69630156	ID=MsG0880046136.01.T01:exon:16953;Parent=MsG0880046136.01.T01
Chr8	exon	69629090	69629282	69629090	ID=MsG0880046136.01.T01:exon:16954;Parent=MsG0880046136.01.T01
Chr8	exon	69627024	69627101	69627024	ID=MsG0880046136.01.T01:exon:16955;Parent=MsG0880046136.01.T01
Chr8	exon	69626877	69626928	69626877	ID=MsG0880046136.01.T01:exon:16956;Parent=MsG0880046136.01.T01
Chr8	exon	69626669	69626790	69626669	ID=MsG0880046136.01.T01:exon:16957;Parent=MsG0880046136.01.T01
Chr8	exon	69626373	69626470	69626373	ID=MsG0880046136.01.T01:exon:16958;Parent=MsG0880046136.01.T01
Chr8	exon	69625822	69625880	69625822	ID=MsG0880046136.01.T01:exon:16959;Parent=MsG0880046136.01.T01
Chr8	exon	69625660	69625716	69625660	ID=MsG0880046136.01.T01:exon:16960;Parent=MsG0880046136.01.T01
Chr8	exon	69625534	69625579	69625534	ID=MsG0880046136.01.T01:exon:16961;Parent=MsG0880046136.01.T01
Chr8	exon	69625341	69625420	69625341	ID=MsG0880046136.01.T01:exon:16962;Parent=MsG0880046136.01.T01
Chr8	exon	69624070	69624148	69624070	ID=MsG0880046136.01.T01:exon:16963;Parent=MsG0880046136.01.T01
Chr8	five_prime_UTR	69634048	69634128	69634048	ID=MsG0880046136.01.T01:five_prime_utr;Parent=MsG0880046136.01.T01
Chr8	CDS	69633964	69634047	69633964	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69633574	69633766	69633574	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69633251	69633328	69633251	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69633071	69633122	69633071	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69632479	69632600	69632479	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69632267	69632364	69632267	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69632101	69632159	69632101	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69631596	69631675	69631596	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69630156	69630202	69630156	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69629090	69629282	69629090	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69627024	69627101	69627024	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69626877	69626928	69626877	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69626669	69626790	69626669	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69626373	69626470	69626373	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69625822	69625880	69625822	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69625660	69625716	69625660	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69625534	69625579	69625534	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69625341	69625420	69625341	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01
Chr8	CDS	69624070	69624148	69624070	ID=MsG0880046136.01.T01:cds;Parent=MsG0880046136.01.T01

Gene Sequence

>MsG0880046136.01.T01

ATGGCAGCAACGGCACCATTGAGTCTTCCAATCATAGACCTCTCCTCACCTGATCGAATTTCCACAGCCAAATCCATCCATCAGGCATGTGTTGAGTATGGTTTCTTTTACCTTGTGAACCACGGTGTGTACGAGGATTTTATCAAACAAGCGTTTGAACAGAGTGCAAAGTTCTTCTCACTTCCAATTGAACACAAAATCAAATTGAACCGCAAGGAGTATAGAGGTTATACTCCTCTCTATGCTGAGAAACTTGACCCTTCTTCACTATCCAAAGGTGATCCAAAAGAGAGCTATTACATTGGCCCTTTAACAGATACCACAAGTGTTCGTCTCAATCAATGGCCTTCTGTTGAATTACTCCCAAATTGGAGACCCACAATTGAATCCTTATATTGGAAAATACTGTCAGCTGGCAAAGATTTGTTGTCTCTAATCGCCCTGTCTTTGAATCTAGATGAAAATTACTTTGAGAAGATCAGTGTCTTAAATAAACCAGAAGCTTTTCTTCGCCTATTGCGCTATCCAGGTGAATTGGGACCTAATGAAGAGATATGTGGTGCCTCTGCTCATTCAGATTATGGCATGATCACTCTCCTATTGACCAACGGTGTTCCGGGACTGCAGATCTGCAAGGAGAAGTTGAAGCAACCACAGGTATGGGAGGATGTGCTTCATGTAGAAGGGTGGCATTCTTCATGGATCCACCCTCAGATTGTGTCGTGGAATGCTTTGAAAGTTGCTGCAGTGAATCATCTCCCCCCAGAATTAAAGTGTCTCGATGAATTTGATGTTAAATCCATACCTAAAAACGCATGTGTTGAGTATGGTTTCTTTTATCTTGTGAACCATGGTGTGGATGAGGATTTTATGAAGCAAGTGTTTGAGCACAGTGCAAAGTTCTTCTCACTTCCACTTCAACACAAAATGAACTTGTCTCGCAAGGAGTACAGAGGTTATACTCCACTCTATGCTGAGAAACTTGATCCTAGTCCACTTTCCAAAGGTGATCCAAAAGAGAGCTACTACATTGGCCCTTTAACAGATACCACAAGTGTTCGTCTCAATCAATGGCCTTGTGATGAATTACTGCCTAATTGGAGACCCACAATGGAATCTTTATTTTGGAAAATACTTTCAGCTGGCAAAGAGTTGCTTTCTCTAATTGCCTTGTCTTTGAGTCTAGATGAAGATCACTTTGAGAAGATAGGTGCCTTAAGCATACCAGAAGTTTTTCTTCGCCTTTTGCACTATCCAGGTGAATTGGGATCTTGTGAAGAGAAATATGGTGCCTCTCCTCATACAGATTATGGCATGATCACTCTCCTATTGACCAACGGTGTTCCAGGACTGCAGATATGCAGGGAGAAGTCCAAGCAACCTCAAGTATGGGAGGATGTTTCTCATGTAGAAGGGGCCTTAATTGTGAACATCGGGGACATGATGGAAAGATGGACAAATTGTTTATACCAGTCAACACTTCACAGGGTGATGCCAATTGGAAAAGAACGTTATTCGGTAGCGTTTTTCATGTATGCATCCTTAGACTGTGTAGTGGAATGCTTTGAAAGTTGTTGCAGTGAATCTTCTCCCTCAAGGTTCCCTCCAATACGTAGTGGGGACTACTTGAATGAAGGGTTTAAACTTATATACGGTTCTGAAAGAGATCTTAATTAA

Protein sequence

>MsG0880046136.01.T01

MAATAPLSLPIIDLSSPDRISTAKSIHQACVEYGFFYLVNHGVYEDFIKQAFEQSAKFFSLPIEHKIKLNRKEYRGYTPLYAEKLDPSSLSKGDPKESYYIGPLTDTTSVRLNQWPSVELLPNWRPTIESLYWKILSAGKDLLSLIALSLNLDENYFEKISVLNKPEAFLRLLRYPGELGPNEEICGASAHSDYGMITLLLTNGVPGLQICKEKLKQPQVWEDVLHVEGWHSSWIHPQIVSWNALKVAAVNHLPPELKCLDEFDVKSIPKNACVEYGFFYLVNHGVDEDFMKQVFEHSAKFFSLPLQHKMNLSRKEYRGYTPLYAEKLDPSPLSKGDPKESYYIGPLTDTTSVRLNQWPCDELLPNWRPTMESLFWKILSAGKELLSLIALSLSLDEDHFEKIGALSIPEVFLRLLHYPGELGSCEEKYGASPHTDYGMITLLLTNGVPGLQICREKSKQPQVWEDVSHVEGALIVNIGDMMERWTNCLYQSTLHRVMPIGKERYSVAFFMYASLDCVVECFESCCSESSPSRFPPIRSGDYLNEGFKLIYGSERDLN*