AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046173.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046173.01.T01 MTR_4g102250 90.196 102 10 0 69 170 266 367 2.04e-49 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046173.01.T01 AT4G22740 59.184 49 18 1 69 115 267 315 9.44e-12 62.8
MsG0880046173.01.T01 AT4G22740 59.184 49 18 1 69 115 267 315 9.44e-12 62.8

Find 90 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTTCGGCCATGCCTTCC+TGG 0.280268 8:-70169266 None:intergenic
AACTTAGTGCAGCTCGTATA+GGG 0.323926 8:+70169500 MsG0880046173.01.T01:three_prime_UTR
GAAGAAGATCAGACATTTGC+AGG 0.344218 8:+70167306 MsG0880046173.01.T01:CDS
CAACTTAGTGCAGCTCGTAT+AGG 0.353635 8:+70169499 MsG0880046173.01.T01:three_prime_UTR
GGTGGACAAGCTGGGCAGGC+TGG 0.358776 8:+70169110 MsG0880046173.01.T01:CDS
GGCAGGCTGGGCAAGGAGGT+TGG 0.363254 8:+70169123 MsG0880046173.01.T01:CDS
AAAGCTTACTCCGTCACTTC+CGG 0.378183 8:-70167461 None:intergenic
AGGTTGGCTGCTCCCTTCAT+TGG 0.382622 8:+70169139 MsG0880046173.01.T01:CDS
GCTAGCCATGGTGGACAAGC+TGG 0.383190 8:+70169101 MsG0880046173.01.T01:CDS
CAACAGTAGAAAATTAATTT+CGG 0.387577 8:-70169280 None:intergenic
ATGCTTTGGTTGGACAGATT+CGG 0.398089 8:+70169435 MsG0880046173.01.T01:three_prime_UTR
TCCTTTAGCAAAATAGAGTA+TGG 0.399953 8:-70169621 None:intergenic
AAAATTATCATGTATACCTA+TGG 0.412045 8:-70169399 None:intergenic
CCATGGTGGACAAGCTGGGC+AGG 0.417626 8:+70169106 MsG0880046173.01.T01:CDS
GTGGACAAGCTGGGCAGGCT+GGG 0.421167 8:+70169111 MsG0880046173.01.T01:CDS
GAAGTATAATATGCTCCATT+TGG 0.424591 8:-70167421 None:intergenic
CATAGCTGACTGTGGAGCTC+TGG 0.438658 8:-70167393 None:intergenic
GAAAGAGAAAAGACACCAAC+AGG 0.440983 8:-70169371 None:intergenic
CAAGCTGGGCAGGCTGGGCA+AGG 0.441828 8:+70169116 MsG0880046173.01.T01:CDS
ACAACCACAAGCAGTATTAA+CGG 0.461223 8:-70169540 None:intergenic
GTCAGCTATGGTGGACCAAA+TGG 0.470607 8:+70167406 MsG0880046173.01.T01:CDS
CAACCACAAGCAGTATTAAC+GGG 0.476550 8:-70169539 None:intergenic
AATTTCGGCCATGCCTTCCT+GGG 0.476935 8:-70169265 None:intergenic
AAGCTGAAACTGCGAGTTTG+AGG 0.483361 8:-70167370 None:intergenic
AAAGGACAGATTCAAATGGC+AGG 0.485013 8:+70169231 MsG0880046173.01.T01:CDS
TAGCTGGCTTTGAAGAAGAA+TGG 0.489121 8:+70167967 MsG0880046173.01.T01:CDS
CCAATACTCCCGCTCCATCC+AGG 0.497687 8:-70168014 None:intergenic
AAATTATCATGTATACCTAT+GGG 0.499545 8:-70169398 None:intergenic
GACACTGTTGCTAAGGTGAA+TGG 0.499961 8:-70169329 None:intergenic
ATCTGGATTAAGTTCTTGAA+AGG 0.500416 8:-70167773 None:intergenic
CATCCAGATGATGAACTTGA+AGG 0.506651 8:+70167160 None:intergenic
ACTTTGCACAACATTAATGA+AGG 0.508117 8:+70167817 MsG0880046173.01.T01:CDS
ACCCACCTTACCTCCAACTT+CGG 0.510156 8:-70169186 None:intergenic
GTCAAAAGTATTTGCCTGGA+TGG 0.514197 8:+70168000 MsG0880046173.01.T01:CDS
GTATACATTGTCCTTGTATG+AGG 0.516603 8:+70169569 MsG0880046173.01.T01:three_prime_UTR
ATAGTATCCACCTTACCATC+TGG 0.517490 8:-70167790 None:intergenic
GGATGACTGTGCTCCAATGA+AGG 0.518769 8:-70169152 None:intergenic
AAGGGTCAAAAGTATTTGCC+TGG 0.518868 8:+70167996 MsG0880046173.01.T01:CDS
ACACTGTTGCTAAGGTGAAT+GGG 0.525351 8:-70169328 None:intergenic
AGAACTTAATCCAGATGGTA+AGG 0.525595 8:+70167780 MsG0880046173.01.T01:CDS
CCTGTGAGAATGTCCGAAGT+TGG 0.528124 8:+70169173 MsG0880046173.01.T01:CDS
TTCTTCTCGTGCTGAAGAGA+AGG 0.528407 8:+70169208 MsG0880046173.01.T01:CDS
CTAGCCATGGTGGACAAGCT+GGG 0.531122 8:+70169102 MsG0880046173.01.T01:CDS
CAGAGCTCCACAGTCAGCTA+TGG 0.534669 8:+70167394 MsG0880046173.01.T01:CDS
CCTGGATGGAGCGGGAGTAT+TGG 0.534924 8:+70168014 MsG0880046173.01.T01:CDS
TCTGGATTAAGTTCTTGAAA+GGG 0.539192 8:-70167772 None:intergenic
AGTATTTGCCTGGATGGAGC+GGG 0.539807 8:+70168006 MsG0880046173.01.T01:CDS
GTCCGAAGTTGGAGGTAAGG+TGG 0.541687 8:+70169184 MsG0880046173.01.T01:CDS
AAGGAAAGGACAGATTCAAA+TGG 0.545943 8:+70169227 MsG0880046173.01.T01:CDS
CAAGATAAACACCTCATACA+AGG 0.547254 8:-70169580 None:intergenic
AATGTCCGAAGTTGGAGGTA+AGG 0.549381 8:+70169181 MsG0880046173.01.T01:CDS
GGTGAATGGGACTTGAGTAC+TGG 0.550113 8:-70169315 None:intergenic
CCTGCCCAGCTTGTCCACCA+TGG 0.556673 8:-70169106 None:intergenic
AGCTCCACAGTCAGCTATGG+TGG 0.556679 8:+70167397 MsG0880046173.01.T01:CDS
TTTCAAGAACTTAATCCAGA+TGG 0.561900 8:+70167775 MsG0880046173.01.T01:CDS
AAGGACAGATTCAAATGGCA+GGG 0.565748 8:+70169232 MsG0880046173.01.T01:CDS
GCTTTGAAGAAGAATGGAAA+GGG 0.568893 8:+70167973 MsG0880046173.01.T01:CDS
AAGTATTTGCCTGGATGGAG+CGG 0.571644 8:+70168005 MsG0880046173.01.T01:CDS
GCATACCACCCAGGAAGGCA+TGG 0.573963 8:+70169257 MsG0880046173.01.T01:CDS
GCTGGGCAGGCTGGGCAAGG+AGG 0.578573 8:+70169119 MsG0880046173.01.T01:CDS
TATTCCATGTTATTACCTGT+TGG 0.586706 8:+70169356 MsG0880046173.01.T01:three_prime_UTR
ACAAGGAGGACCGGAAGTGA+CGG 0.587602 8:+70167451 MsG0880046173.01.T01:CDS
ATCATGTATACCTATGGGGA+CGG 0.591939 8:-70169393 None:intergenic
CTCGTGCTGAAGAGAAGGAA+AGG 0.592713 8:+70169213 MsG0880046173.01.T01:CDS
TTCGGCCATGCCTTCCTGGG+TGG 0.594518 8:-70169262 None:intergenic
CCAACTTCGGACATTCTCAC+AGG 0.596498 8:-70169173 None:intergenic
TCCGAAGTTGGAGGTAAGGT+GGG 0.596962 8:+70169185 MsG0880046173.01.T01:CDS
GATGACTGTGCTCCAATGAA+GGG 0.597405 8:-70169151 None:intergenic
CTGCCCGTTAATACTGCTTG+TGG 0.602628 8:+70169536 MsG0880046173.01.T01:three_prime_UTR
GTTGTACAGATCTCAGACGC+TGG 0.605125 8:-70169480 None:intergenic
TCATCAAAGACAAGGAGGAC+CGG 0.605585 8:+70167442 MsG0880046173.01.T01:CDS
ATATCATGACACTGTTGCTA+AGG 0.606244 8:-70169336 None:intergenic
ACTTAGTGCAGCTCGTATAG+GGG 0.608189 8:+70169501 MsG0880046173.01.T01:three_prime_UTR
TGAAGAAGAATGGAAAGGGA+AGG 0.611158 8:+70167977 MsG0880046173.01.T01:CDS
GGCTTTGAAGAAGAATGGAA+AGG 0.613021 8:+70167972 MsG0880046173.01.T01:CDS
GGGGTGCATACCACCCAGGA+AGG 0.618402 8:+70169252 MsG0880046173.01.T01:CDS
GTATGGCATAGAAGTTGCAC+GGG 0.619372 8:-70169604 None:intergenic
GTGAGAATGTCCGAAGTTGG+AGG 0.621493 8:+70169176 MsG0880046173.01.T01:CDS
AGTATGGCATAGAAGTTGCA+CGG 0.624472 8:-70169605 None:intergenic
AGGACAGATTCAAATGGCAG+GGG 0.624927 8:+70169233 MsG0880046173.01.T01:CDS
GAAGAAGAATGGAAAGGGAA+GGG 0.632258 8:+70167978 MsG0880046173.01.T01:CDS
ACTTAATCCAGATGGTAAGG+TGG 0.637514 8:+70167783 MsG0880046173.01.T01:CDS
GACACCAACAGGTAATAACA+TGG 0.637913 8:-70169360 None:intergenic
AAGAAGATCAGACATTTGCA+GGG 0.640119 8:+70167307 MsG0880046173.01.T01:CDS
GGCAGGGGTGCATACCACCC+AGG 0.649462 8:+70169248 MsG0880046173.01.T01:CDS
TGGTCCACCATAGCTGACTG+TGG 0.666212 8:-70167401 None:intergenic
AATTATCATGTATACCTATG+GGG 0.672459 8:-70169397 None:intergenic
GCATCACTATAACATTTCAG+TGG 0.677933 8:-70169458 None:intergenic
ATTATACTTCATCAAAGACA+AGG 0.695492 8:+70167434 MsG0880046173.01.T01:CDS
ATACTTCATCAAAGACAAGG+AGG 0.753490 8:+70167437 MsG0880046173.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATGATAAATTATGATTA+GGG + Chr8:70168843-70168862 MsG0880046173.01.T01:intron 10.0%
!! TTAAATGATAAATTATGATT+AGG + Chr8:70168842-70168861 MsG0880046173.01.T01:intron 10.0%
!! AAAGTAATTCAATTAAAGTA+AGG - Chr8:70167728-70167747 None:intergenic 15.0%
!! AAATGATAAATTATGATTAG+GGG + Chr8:70168844-70168863 MsG0880046173.01.T01:intron 15.0%
!!! ACTTTTATCTAATATTTTGT+AGG + Chr8:70167743-70167762 MsG0880046173.01.T01:intron 15.0%
!!! CTTTTATCTAATATTTTGTA+GGG + Chr8:70167744-70167763 MsG0880046173.01.T01:intron 15.0%
!!! TAAACTATTTTATGAGATTA+AGG - Chr8:70168900-70168919 None:intergenic 15.0%
!!! TTTTATCTAATATTTTGTAG+GGG + Chr8:70167745-70167764 MsG0880046173.01.T01:intron 15.0%
!! AAAATTATCATGTATACCTA+TGG - Chr8:70169402-70169421 None:intergenic 20.0%
!! AAATTATCATGTATACCTAT+GGG - Chr8:70169401-70169420 None:intergenic 20.0%
!! AACTGATTAATTGAGAAATA+TGG + Chr8:70168042-70168061 MsG0880046173.01.T01:intron 20.0%
!! AGATACATTGTAACATAAAA+CGG - Chr8:70168360-70168379 None:intergenic 20.0%
!! ATAGTAATATATACATGATG+TGG + Chr8:70168867-70168886 MsG0880046173.01.T01:intron 20.0%
!! CAACAGTAGAAAATTAATTT+CGG - Chr8:70169283-70169302 None:intergenic 20.0%
!! GGAAATTTGGAAATAAAAAA+TGG - Chr8:70168923-70168942 None:intergenic 20.0%
!! TAGTAATATATACATGATGT+GGG + Chr8:70168868-70168887 MsG0880046173.01.T01:intron 20.0%
!! TTATGTAAAAAAAATGAGAC+AGG - Chr8:70168944-70168963 None:intergenic 20.0%
!! TTATGTATATGGTATTATGT+CGG + Chr8:70169008-70169027 MsG0880046173.01.T01:intron 20.0%
!!! ACAAACTACAATTTTTTAGA+GGG - Chr8:70167241-70167260 None:intergenic 20.0%
!!! ATTGTAGTTTGTATTTGTTT+TGG + Chr8:70167249-70167268 MsG0880046173.01.T01:intron 20.0%
!!! TACAAACTACAATTTTTTAG+AGG - Chr8:70167242-70167261 None:intergenic 20.0%
!!! TAGTTTTTTTTCTTCTTTTC+TGG + Chr8:70167856-70167875 MsG0880046173.01.T01:intron 20.0%
!!! TGTTTTGGCTAATTTTTATT+GGG + Chr8:70167264-70167283 MsG0880046173.01.T01:intron 20.0%
!!! TTGTTTTGGCTAATTTTTAT+TGG + Chr8:70167263-70167282 MsG0880046173.01.T01:intron 20.0%
! AAAAAATGAGACAGGAAATT+TGG - Chr8:70168936-70168955 None:intergenic 25.0%
! AATAGATGTGTGAAAATATG+TGG - Chr8:70168474-70168493 None:intergenic 25.0%
! AATTATCATGTATACCTATG+GGG - Chr8:70169400-70169419 None:intergenic 25.0%
! ACAGGTTAAAATGATTCAAA+TGG - Chr8:70169062-70169081 None:intergenic 25.0%
! ACTTGAATACTTGAAATACA+AGG + Chr8:70168213-70168232 MsG0880046173.01.T01:intron 25.0%
! ATACATTGTAACATAAAACG+GGG - Chr8:70168358-70168377 None:intergenic 25.0%
! ATTATACTTCATCAAAGACA+AGG + Chr8:70167434-70167453 MsG0880046173.01.T01:CDS 25.0%
! GATACATTGTAACATAAAAC+GGG - Chr8:70168359-70168378 None:intergenic 25.0%
! TAAACTGCGCTAAAAAAATT+TGG - Chr8:70168815-70168834 None:intergenic 25.0%
! TCTTTCGTTAAACTGATTAT+TGG - Chr8:70168331-70168350 None:intergenic 25.0%
! TTGTAACATATATCATCACT+CGG - Chr8:70168255-70168274 None:intergenic 25.0%
! TTTATGTTACAATGTATCTG+TGG + Chr8:70168361-70168380 MsG0880046173.01.T01:intron 25.0%
!! ACTGTTTAGAATTAACCAAT+AGG + Chr8:70167194-70167213 MsG0880046173.01.T01:intron 25.0%
!! CTGTTTAGAATTAACCAATA+GGG + Chr8:70167195-70167214 MsG0880046173.01.T01:intron 25.0%
!! GCTCTTTATTATTGACTAAT+GGG + Chr8:70168699-70168718 MsG0880046173.01.T01:intron 25.0%
!! TGCTCTTTATTATTGACTAA+TGG + Chr8:70168698-70168717 MsG0880046173.01.T01:intron 25.0%
!! TGTTTAGAATTAACCAATAG+GGG + Chr8:70167196-70167215 MsG0880046173.01.T01:intron 25.0%
!!! GATATTATATTGAGTTGGTT+TGG + Chr8:70167656-70167675 MsG0880046173.01.T01:intron 25.0%
!!! TTTTTGGCACAAGTATATAA+AGG - Chr8:70168120-70168139 None:intergenic 25.0%
!!! TTTTTTTTCTTCTTTTCTGG+TGG + Chr8:70167859-70167878 MsG0880046173.01.T01:intron 25.0%
ACTTTGCACAACATTAATGA+AGG + Chr8:70167817-70167836 MsG0880046173.01.T01:CDS 30.0%
ATATAATATCGCTACCAAAG+AGG - Chr8:70167646-70167665 None:intergenic 30.0%
ATCTGGATTAAGTTCTTGAA+AGG - Chr8:70167776-70167795 None:intergenic 30.0%
ATTCAATTAAAGTAAGGACG+TGG - Chr8:70167722-70167741 None:intergenic 30.0%
CCTAAAACATAAACAAGAAC+AGG - Chr8:70169080-70169099 None:intergenic 30.0%
GAAAAATTGCAAATACTGCA+TGG - Chr8:70168306-70168325 None:intergenic 30.0%
GAAGTATAATATGCTCCATT+TGG - Chr8:70167424-70167443 None:intergenic 30.0%
GTAGCGATATTATATTGAGT+TGG + Chr8:70167651-70167670 MsG0880046173.01.T01:intron 30.0%
TATTCCATGTTATTACCTGT+TGG + Chr8:70169356-70169375 MsG0880046173.01.T01:three_prime_UTR 30.0%
TCCTTTAGCAAAATAGAGTA+TGG - Chr8:70169624-70169643 None:intergenic 30.0%
TCTGGATTAAGTTCTTGAAA+GGG - Chr8:70167775-70167794 None:intergenic 30.0%
TTGTGAAATCGTTATCATGT+TGG + Chr8:70168604-70168623 MsG0880046173.01.T01:intron 30.0%
TTTACAGCTAGACTAAATCA+AGG + Chr8:70167492-70167511 MsG0880046173.01.T01:intron 30.0%
TTTCAAGAACTTAATCCAGA+TGG + Chr8:70167775-70167794 MsG0880046173.01.T01:CDS 30.0%
! AATAATAAAGAGCATGAGCT+AGG - Chr8:70168692-70168711 None:intergenic 30.0%
! ACTAACTTCACAGAACTTTT+TGG - Chr8:70168136-70168155 None:intergenic 30.0%
! TAGCTGTATCTTTTATGCTT+TGG + Chr8:70169421-70169440 MsG0880046173.01.T01:three_prime_UTR 30.0%
! TGATAACGATTTCACAACAA+TGG - Chr8:70168601-70168620 None:intergenic 30.0%
! TGGCTAATTTTTATTGGGTT+GGG + Chr8:70167269-70167288 MsG0880046173.01.T01:intron 30.0%
! TGTATCTTTTATGCTTTGGT+TGG + Chr8:70169425-70169444 MsG0880046173.01.T01:three_prime_UTR 30.0%
! TTATTGACTAATGGGAAGTA+AGG + Chr8:70168707-70168726 MsG0880046173.01.T01:intron 30.0%
! TTGGCTAATTTTTATTGGGT+TGG + Chr8:70167268-70167287 MsG0880046173.01.T01:intron 30.0%
!! CCTGTTCTTGTTTATGTTTT+AGG + Chr8:70169077-70169096 MsG0880046173.01.T01:intron 30.0%
!!! ATATTGAGTTGGTTTGGTTT+TGG + Chr8:70167662-70167681 MsG0880046173.01.T01:intron 30.0%
!!! TATTGAGTTGGTTTGGTTTT+GGG + Chr8:70167663-70167682 MsG0880046173.01.T01:intron 30.0%
AAAAAAACTGCCAACACAAC+AGG - Chr8:70168790-70168809 None:intergenic 35.0%
AAGAAGATCAGACATTTGCA+GGG + Chr8:70167307-70167326 MsG0880046173.01.T01:CDS 35.0%
AAGGAAAGGACAGATTCAAA+TGG + Chr8:70169227-70169246 MsG0880046173.01.T01:CDS 35.0%
ACAACCACAAGCAGTATTAA+CGG - Chr8:70169543-70169562 None:intergenic 35.0%
AGAACTTAATCCAGATGGTA+AGG + Chr8:70167780-70167799 MsG0880046173.01.T01:CDS 35.0%
AGTTGCAGAGAACTGAAATA+GGG - Chr8:70168450-70168469 None:intergenic 35.0%
ATACTTCATCAAAGACAAGG+AGG + Chr8:70167437-70167456 MsG0880046173.01.T01:CDS 35.0%
ATATCATGACACTGTTGCTA+AGG - Chr8:70169339-70169358 None:intergenic 35.0%
ATTCAATGGTGTAGAGAGTA+TGG + Chr8:70168755-70168774 MsG0880046173.01.T01:intron 35.0%
CAAGATAAACACCTCATACA+AGG - Chr8:70169583-70169602 None:intergenic 35.0%
CCAATGTAGAATTCAATGTG+TGG + Chr8:70168977-70168996 MsG0880046173.01.T01:intron 35.0%
CCACACATTGAATTCTACAT+TGG - Chr8:70168980-70168999 None:intergenic 35.0%
GATTCCATCTTCATTGTGTT+AGG + Chr8:70167536-70167555 MsG0880046173.01.T01:intron 35.0%
GCATCACTATAACATTTCAG+TGG - Chr8:70169461-70169480 None:intergenic 35.0%
GTATACATTGTCCTTGTATG+AGG + Chr8:70169569-70169588 MsG0880046173.01.T01:three_prime_UTR 35.0%
TAGTTGCAGAGAACTGAAAT+AGG - Chr8:70168451-70168470 None:intergenic 35.0%
TTGTATGTCAGCATTACTGA+AGG + Chr8:70167917-70167936 MsG0880046173.01.T01:intron 35.0%
! GCCATACTCTATTTTGCTAA+AGG + Chr8:70169620-70169639 MsG0880046173.01.T01:three_prime_UTR 35.0%
!! CTTTTGCTGTGTTTCTTTAG+TGG + Chr8:70167690-70167709 MsG0880046173.01.T01:intron 35.0%
!! GCTTTGAAGAAGAATGGAAA+GGG + Chr8:70167973-70167992 MsG0880046173.01.T01:CDS 35.0%
!! GGCTAATTTTTATTGGGTTG+GGG + Chr8:70167270-70167289 MsG0880046173.01.T01:intron 35.0%
!!! GCTAATTTTTATTGGGTTGG+GGG + Chr8:70167271-70167290 MsG0880046173.01.T01:intron 35.0%
AAAGGACAGATTCAAATGGC+AGG + Chr8:70169231-70169250 MsG0880046173.01.T01:CDS 40.0%
AACTTAGTGCAGCTCGTATA+GGG + Chr8:70169500-70169519 MsG0880046173.01.T01:three_prime_UTR 40.0%
AAGGACAGATTCAAATGGCA+GGG + Chr8:70169232-70169251 MsG0880046173.01.T01:CDS 40.0%
AATGGGAAGTAAGGAAACAG+TGG + Chr8:70168716-70168735 MsG0880046173.01.T01:intron 40.0%
ACACTGTTGCTAAGGTGAAT+GGG - Chr8:70169331-70169350 None:intergenic 40.0%
ACTTAATCCAGATGGTAAGG+TGG + Chr8:70167783-70167802 MsG0880046173.01.T01:CDS 40.0%
AGTATGGCATAGAAGTTGCA+CGG - Chr8:70169608-70169627 None:intergenic 40.0%
ATAGTATCCACCTTACCATC+TGG - Chr8:70167793-70167812 None:intergenic 40.0%
ATCATGTATACCTATGGGGA+CGG - Chr8:70169396-70169415 None:intergenic 40.0%
ATGCTTTGGTTGGACAGATT+CGG + Chr8:70169435-70169454 MsG0880046173.01.T01:three_prime_UTR 40.0%
ATTGTGTTAGGTGACACTTG+AGG + Chr8:70167548-70167567 MsG0880046173.01.T01:intron 40.0%
CAACCACAAGCAGTATTAAC+GGG - Chr8:70169542-70169561 None:intergenic 40.0%
GAAAGAGAAAAGACACCAAC+AGG - Chr8:70169374-70169393 None:intergenic 40.0%
GAAGAAGAATGGAAAGGGAA+GGG + Chr8:70167978-70167997 MsG0880046173.01.T01:CDS 40.0%
GAAGAAGATCAGACATTTGC+AGG + Chr8:70167306-70167325 MsG0880046173.01.T01:CDS 40.0%
GACACCAACAGGTAATAACA+TGG - Chr8:70169363-70169382 None:intergenic 40.0%
GAGTGAAATGCTTTCTCATG+GGG + Chr8:70168555-70168574 MsG0880046173.01.T01:intron 40.0%
GGAGTGAAATGCTTTCTCAT+GGG + Chr8:70168554-70168573 MsG0880046173.01.T01:intron 40.0%
GTCACCTAACACAATGAAGA+TGG - Chr8:70167543-70167562 None:intergenic 40.0%
GTTGCAGAGAACTGAAATAG+GGG - Chr8:70168449-70168468 None:intergenic 40.0%
TGAAGAAGAATGGAAAGGGA+AGG + Chr8:70167977-70167996 MsG0880046173.01.T01:CDS 40.0%
TGGAGTGAAATGCTTTCTCA+TGG + Chr8:70168553-70168572 MsG0880046173.01.T01:intron 40.0%
TGGCACACGAGTTATGTATA+TGG + Chr8:70168997-70169016 MsG0880046173.01.T01:intron 40.0%
TTGCAGAGAACTGAAATAGG+GGG - Chr8:70168448-70168467 None:intergenic 40.0%
! GCTTCTCACAGAATTTCTAG+AGG + Chr8:70167603-70167622 MsG0880046173.01.T01:intron 40.0%
! TGGCATACTGCTAACAGTTT+TGG - Chr8:70169042-70169061 None:intergenic 40.0%
! TTTTTCGCAGATCAACTAGC+TGG + Chr8:70167951-70167970 MsG0880046173.01.T01:intron 40.0%
!! AAGGGTCAAAAGTATTTGCC+TGG + Chr8:70167996-70168015 MsG0880046173.01.T01:CDS 40.0%
!! GAGAAGTACTGTGCATTCAA+TGG + Chr8:70168741-70168760 MsG0880046173.01.T01:intron 40.0%
!! GGCTTTGAAGAAGAATGGAA+AGG + Chr8:70167972-70167991 MsG0880046173.01.T01:CDS 40.0%
!! GTCAAAAGTATTTGCCTGGA+TGG + Chr8:70168000-70168019 MsG0880046173.01.T01:CDS 40.0%
!! TAGCTGGCTTTGAAGAAGAA+TGG + Chr8:70167967-70167986 MsG0880046173.01.T01:CDS 40.0%
!!! TATGTTTTAGGAGCTAGCCA+TGG + Chr8:70169089-70169108 MsG0880046173.01.T01:intron 40.0%
AAAGCTTACTCCGTCACTTC+CGG - Chr8:70167464-70167483 None:intergenic 45.0%
AAGAGGATACCTTGTCATGC+AGG - Chr8:70167629-70167648 None:intergenic 45.0%
AAGCTGAAACTGCGAGTTTG+AGG - Chr8:70167373-70167392 None:intergenic 45.0%
AAGTATTTGCCTGGATGGAG+CGG + Chr8:70168005-70168024 MsG0880046173.01.T01:CDS 45.0%
AATGTCCGAAGTTGGAGGTA+AGG + Chr8:70169181-70169200 MsG0880046173.01.T01:CDS 45.0%
ACTTAGTGCAGCTCGTATAG+GGG + Chr8:70169501-70169520 MsG0880046173.01.T01:three_prime_UTR 45.0%
AGGACAGATTCAAATGGCAG+GGG + Chr8:70169233-70169252 MsG0880046173.01.T01:CDS 45.0%
CAACTTAGTGCAGCTCGTAT+AGG + Chr8:70169499-70169518 MsG0880046173.01.T01:three_prime_UTR 45.0%
GACACTGTTGCTAAGGTGAA+TGG - Chr8:70169332-70169351 None:intergenic 45.0%
GATGACTGTGCTCCAATGAA+GGG - Chr8:70169154-70169173 None:intergenic 45.0%
GCTTAGTATGCCTGTTGTGT+TGG + Chr8:70168777-70168796 MsG0880046173.01.T01:intron 45.0%
GTATGGCATAGAAGTTGCAC+GGG - Chr8:70169607-70169626 None:intergenic 45.0%
TCATCAAAGACAAGGAGGAC+CGG + Chr8:70167442-70167461 MsG0880046173.01.T01:CDS 45.0%
TTCTTCTCGTGCTGAAGAGA+AGG + Chr8:70169208-70169227 MsG0880046173.01.T01:CDS 45.0%
! CAAAAAGCACAAGCCCCTAT+TGG - Chr8:70167212-70167231 None:intergenic 45.0%
! GCATGACAAGGTATCCTCTT+TGG + Chr8:70167629-70167648 MsG0880046173.01.T01:intron 45.0%
! GTGCTCACATGTAGCTTGTA+TGG + Chr8:70168533-70168552 MsG0880046173.01.T01:intron 45.0%
! TTTTCTCTTTCCGTCCCCAT+AGG + Chr8:70169383-70169402 MsG0880046173.01.T01:three_prime_UTR 45.0%
AATTTCGGCCATGCCTTCCT+GGG - Chr8:70169268-70169287 None:intergenic 50.0%
ACCCACCTTACCTCCAACTT+CGG - Chr8:70169189-70169208 None:intergenic 50.0%
AGGAGGCTGATACTTCCACA+AGG + Chr8:70167577-70167596 MsG0880046173.01.T01:intron 50.0%
AGTATTTGCCTGGATGGAGC+GGG + Chr8:70168006-70168025 MsG0880046173.01.T01:CDS 50.0%
CCAACTTCGGACATTCTCAC+AGG - Chr8:70169176-70169195 None:intergenic 50.0%
CCTGTGAGAATGTCCGAAGT+TGG + Chr8:70169173-70169192 MsG0880046173.01.T01:CDS 50.0%
CTCGTGCTGAAGAGAAGGAA+AGG + Chr8:70169213-70169232 MsG0880046173.01.T01:CDS 50.0%
CTGCCCGTTAATACTGCTTG+TGG + Chr8:70169536-70169555 MsG0880046173.01.T01:three_prime_UTR 50.0%
GGATGACTGTGCTCCAATGA+AGG - Chr8:70169155-70169174 None:intergenic 50.0%
GGTGAATGGGACTTGAGTAC+TGG - Chr8:70169318-70169337 None:intergenic 50.0%
GTGAGAATGTCCGAAGTTGG+AGG + Chr8:70169176-70169195 MsG0880046173.01.T01:CDS 50.0%
GTTGTACAGATCTCAGACGC+TGG - Chr8:70169483-70169502 None:intergenic 50.0%
TAATTTCGGCCATGCCTTCC+TGG - Chr8:70169269-70169288 None:intergenic 50.0%
TCCGAAGTTGGAGGTAAGGT+GGG + Chr8:70169185-70169204 MsG0880046173.01.T01:CDS 50.0%
TCTGTGAGAAGCTTGCCTTG+TGG - Chr8:70167595-70167614 None:intergenic 50.0%
! TTCTAGAGGCCTGCATGACA+AGG + Chr8:70167617-70167636 MsG0880046173.01.T01:intron 50.0%
!! GTCAGCTATGGTGGACCAAA+TGG + Chr8:70167406-70167425 MsG0880046173.01.T01:CDS 50.0%
!!! GTTTTAGGAGCTAGCCATGG+TGG + Chr8:70169092-70169111 MsG0880046173.01.T01:intron 50.0%
ACAAGGAGGACCGGAAGTGA+CGG + Chr8:70167451-70167470 MsG0880046173.01.T01:CDS 55.0%
AGCTCCACAGTCAGCTATGG+TGG + Chr8:70167397-70167416 MsG0880046173.01.T01:CDS 55.0%
CAGAGCTCCACAGTCAGCTA+TGG + Chr8:70167394-70167413 MsG0880046173.01.T01:CDS 55.0%
CATAGCTGACTGTGGAGCTC+TGG - Chr8:70167396-70167415 None:intergenic 55.0%
GACACTTGAGGAGAGCAAGG+AGG + Chr8:70167560-70167579 MsG0880046173.01.T01:intron 55.0%
GGTGACACTTGAGGAGAGCA+AGG + Chr8:70167557-70167576 MsG0880046173.01.T01:intron 55.0%
GTCCGAAGTTGGAGGTAAGG+TGG + Chr8:70169184-70169203 MsG0880046173.01.T01:CDS 55.0%
TGGTCCACCATAGCTGACTG+TGG - Chr8:70167404-70167423 None:intergenic 55.0%
! AGGTTGGCTGCTCCCTTCAT+TGG + Chr8:70169139-70169158 MsG0880046173.01.T01:CDS 55.0%
!! CTAGCCATGGTGGACAAGCT+GGG + Chr8:70169102-70169121 MsG0880046173.01.T01:CDS 55.0%
CCAATACTCCCGCTCCATCC+AGG - Chr8:70168017-70168036 None:intergenic 60.0%
GCATACCACCCAGGAAGGCA+TGG + Chr8:70169257-70169276 MsG0880046173.01.T01:CDS 60.0%
! CCTGGATGGAGCGGGAGTAT+TGG + Chr8:70168014-70168033 MsG0880046173.01.T01:CDS 60.0%
! GGGTTGGGGGTCAAGTTGCA+GGG + Chr8:70167284-70167303 MsG0880046173.01.T01:intron 60.0%
! TGGGTTGGGGGTCAAGTTGC+AGG + Chr8:70167283-70167302 MsG0880046173.01.T01:intron 60.0%
!! GCTAGCCATGGTGGACAAGC+TGG + Chr8:70169101-70169120 MsG0880046173.01.T01:CDS 60.0%
CCTGCCCAGCTTGTCCACCA+TGG - Chr8:70169109-70169128 None:intergenic 65.0%
GGGGTGCATACCACCCAGGA+AGG + Chr8:70169252-70169271 MsG0880046173.01.T01:CDS 65.0%
GTGGACAAGCTGGGCAGGCT+GGG + Chr8:70169111-70169130 MsG0880046173.01.T01:CDS 65.0%
TTCGGCCATGCCTTCCTGGG+TGG - Chr8:70169265-70169284 None:intergenic 65.0%
!! CCATGGTGGACAAGCTGGGC+AGG + Chr8:70169106-70169125 MsG0880046173.01.T01:CDS 65.0%
CAAGCTGGGCAGGCTGGGCA+AGG + Chr8:70169116-70169135 MsG0880046173.01.T01:CDS 70.0%
GGCAGGCTGGGCAAGGAGGT+TGG + Chr8:70169123-70169142 MsG0880046173.01.T01:CDS 70.0%
GGTGGACAAGCTGGGCAGGC+TGG + Chr8:70169110-70169129 MsG0880046173.01.T01:CDS 70.0%
! GGCAGGGGTGCATACCACCC+AGG + Chr8:70169248-70169267 MsG0880046173.01.T01:CDS 70.0%
GCTGGGCAGGCTGGGCAAGG+AGG + Chr8:70169119-70169138 MsG0880046173.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 70167167 70169684 70167167 ID=MsG0880046173.01;Name=MsG0880046173.01
Chr8 mRNA 70167167 70169684 70167167 ID=MsG0880046173.01.T01;Parent=MsG0880046173.01;Name=MsG0880046173.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0.8|1|1|0.6|0.5|6|395|176
Chr8 exon 70167167 70167181 70167167 ID=MsG0880046173.01.T01:exon:27562;Parent=MsG0880046173.01.T01
Chr8 exon 70167299 70167474 70167299 ID=MsG0880046173.01.T01:exon:27563;Parent=MsG0880046173.01.T01
Chr8 exon 70167765 70167838 70167765 ID=MsG0880046173.01.T01:exon:27564;Parent=MsG0880046173.01.T01
Chr8 exon 70167961 70168035 70167961 ID=MsG0880046173.01.T01:exon:27565;Parent=MsG0880046173.01.T01
Chr8 exon 70169099 70169684 70169099 ID=MsG0880046173.01.T01:exon:27566;Parent=MsG0880046173.01.T01
Chr8 CDS 70167167 70167181 70167167 ID=MsG0880046173.01.T01:cds;Parent=MsG0880046173.01.T01
Chr8 CDS 70167299 70167474 70167299 ID=MsG0880046173.01.T01:cds;Parent=MsG0880046173.01.T01
Chr8 CDS 70167765 70167838 70167765 ID=MsG0880046173.01.T01:cds;Parent=MsG0880046173.01.T01
Chr8 CDS 70167961 70168035 70167961 ID=MsG0880046173.01.T01:cds;Parent=MsG0880046173.01.T01
Chr8 CDS 70169099 70169289 70169099 ID=MsG0880046173.01.T01:cds;Parent=MsG0880046173.01.T01
Chr8 three_prime_UTR 70169290 70169684 70169290 ID=MsG0880046173.01.T01:three_prime_utr;Parent=MsG0880046173.01.T01
Gene Sequence

>MsG0880046173.01.T01

ATGATGAACTTGAAGTTGCAGGGAAGAAGATCAGACATTTGCAGGGTAGAAATGATACAACAGAGTACAACAGCAAGCAGCTTCAGCCTCAAACTCGCAGTTTCAGCTTCCAGAGCTCCACAGTCAGCTATGGTGGACCAAATGGAGCATATTATACTTCATCAAAGACAAGGAGGACCGGAAGTGACGGAGGGCACACCCTTTCAAGAACTTAATCCAGATGGTAAGGTGGATACTATGCAGACTTTGCACAACATTAATGAAGATCAACTAGCTGGCTTTGAAGAAGAATGGAAAGGGAAGGGTCAAAAGTATTTGCCTGGATGGAGCGGGAGTATTGGAGCTAGCCATGGTGGACAAGCTGGGCAGGCTGGGCAAGGAGGTTGGCTGCTCCCTTCATTGGAGCACAGTCATCCTGTGAGAATGTCCGAAGTTGGAGGTAAGGTGGGTTCTTCTCGTGCTGAAGAGAAGGAAAGGACAGATTCAAATGGCAGGGGTGCATACCACCCAGGAAGGCATGGCCGAAATTAA
Protein sequence

>MsG0880046173.01.T01

MMNLKLQGRRSDICRVEMIQQSTTASSFSLKLAVSASRAPQSAMVDQMEHIILHQRQGGPEVTEGTPFQELNPDGKVDTMQTLHNINEDQLAGFEEEWKGKGQKYLPGWSGSIGASHGGQAGQAGQGGWLLPSLEHSHPVRMSEVGGKVGSSRAEEKERTDSNGRGAYHPGRHGRN*