AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046282.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046282.01.T01 MTR_4g104760 98.083 313 5 1 1 313 1 312 0.0 631
MsG0880046282.01.T01 MTR_3g076610 71.182 347 58 8 1 313 1 339 9.71e-172 479
MsG0880046282.01.T01 MTR_5g069570 47.119 295 134 5 26 313 30 309 2.45e-89 269
MsG0880046282.01.T01 MTR_3g082430 44.558 294 145 6 26 313 12 293 1.35e-80 246
MsG0880046282.01.T01 MTR_5g096570 40.984 305 161 5 13 313 1173 1462 1.71e-64 220
MsG0880046282.01.T01 MTR_7g095100 38.305 295 160 6 27 313 27 307 3.35e-62 200
MsG0880046282.01.T01 MTR_1g081260 34.069 317 182 7 7 313 4 303 2.45e-59 192
MsG0880046282.01.T01 MTR_7g114790 35.220 318 176 9 7 311 4 304 1.38e-58 190
MsG0880046282.01.T01 MTR_5g069550 41.414 198 96 5 4 199 10 189 6.10e-43 146
MsG0880046282.01.T01 MTR_2g084965 33.333 249 152 6 24 267 35 274 3.85e-41 145
MsG0880046282.01.T01 MTR_4g055230 32.824 262 156 7 9 267 27 271 1.17e-39 141
MsG0880046282.01.T01 MTR_3g106250 30.357 280 151 9 7 267 6 260 4.17e-37 135
MsG0880046282.01.T01 MTR_1g013100 31.915 282 148 13 4 267 5 260 1.45e-33 125
MsG0880046282.01.T01 MTR_7g103900 28.444 225 150 3 47 267 56 273 4.23e-28 110
MsG0880046282.01.T01 MTR_7g103900 28.767 146 103 1 47 191 56 201 4.04e-14 70.9
MsG0880046282.01.T01 MTR_5g069590 34.375 96 35 2 95 190 26 93 2.31e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046282.01.T01 AT4G12690 47.855 303 132 6 12 313 8 285 6.04e-92 275
MsG0880046282.01.T01 AT4G12690 47.855 303 132 6 12 313 8 285 6.04e-92 275
MsG0880046282.01.T01 AT2G04220 45.130 308 147 6 14 313 14 307 1.50e-87 265
MsG0880046282.01.T01 AT5G48270 45.152 330 137 10 8 313 13 322 7.79e-85 258
MsG0880046282.01.T01 AT5G28150 39.236 288 150 6 27 313 26 289 5.40e-67 211
MsG0880046282.01.T01 AT3G04860 36.877 301 139 8 27 313 26 289 3.87e-63 202
MsG0880046282.01.T01 AT3G13229 37.500 240 131 6 36 268 11 238 1.74e-49 166
MsG0880046282.01.T01 AT2G27770 33.904 292 170 9 28 312 45 320 1.14e-41 147
MsG0880046282.01.T01 AT5G11000 37.500 192 92 6 85 267 100 272 3.52e-33 126
MsG0880046282.01.T01 AT2G25200 29.927 274 137 9 7 255 14 257 3.01e-24 101
MsG0880046282.01.T01 AT2G36470 27.636 275 153 9 6 259 3 252 1.96e-18 84.7

Find 61 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTGAATTTAGCAGCTTTA+AGG 0.146488 8:+71518688 None:intergenic
AAATGAGAATTGAAGTTTAT+TGG 0.176442 8:-71518287 MsG0880046282.01.T01:CDS
GTATATTGTGCTTCTCCTTC+TGG 0.183600 8:+71518807 None:intergenic
TCAAAGAAAGAGCATATATT+TGG 0.217802 8:-71518528 MsG0880046282.01.T01:CDS
TTCCTTGCTTGAATTGATAA+TGG 0.265556 8:+71518168 None:intergenic
AATGAGAATTGAAGTTTATT+GGG 0.266965 8:-71518286 MsG0880046282.01.T01:CDS
AGCTCTTATTGATCCTATAT+TGG 0.278455 8:-71518553 MsG0880046282.01.T01:CDS
GAAGAGGTTGTTCTTCTTCT+TGG 0.286718 8:-71518618 MsG0880046282.01.T01:CDS
TGGCTTTAGTTCAACCTTGC+AGG 0.306793 8:+71518785 None:intergenic
TCAAAACGGTTTATAGTTGA+TGG 0.314981 8:-71518738 MsG0880046282.01.T01:CDS
TCTTTCTTTGAAACCAATAT+AGG 0.324966 8:+71518540 None:intergenic
ATCTCCATCTCTGGCTGAAC+TGG 0.326566 8:+71518390 None:intergenic
GAAATAGTAGAAGACAAAAT+TGG 0.352145 8:+71518207 None:intergenic
CATTTGCAATGGAAGTTTAG+AGG 0.360380 8:-71518333 MsG0880046282.01.T01:CDS
TTTGCTTGTTTCTTTATGCT+TGG 0.399365 8:-71518101 MsG0880046282.01.T01:CDS
CTTGTTTCTTTATGCTTGGA+AGG 0.403606 8:-71518097 MsG0880046282.01.T01:CDS
CATGATTGGTTATTTAGTCC+TGG 0.407759 8:-71518258 MsG0880046282.01.T01:CDS
TCAATTGAATGCAAGAATAG+AGG 0.423284 8:-71518447 MsG0880046282.01.T01:CDS
AAACATGCTTATAGAAGAAC+AGG 0.424820 8:-71518585 MsG0880046282.01.T01:CDS
GATAAGAATTGATGGACATT+TGG 0.428617 8:-71518370 MsG0880046282.01.T01:CDS
TTTATTGGGATGTTCATGAT+TGG 0.432268 8:-71518272 MsG0880046282.01.T01:CDS
GAATGCAAGAATAGAGGAAA+TGG 0.432427 8:-71518441 MsG0880046282.01.T01:CDS
GGAGGCGGTGTGACACGTTC+CGG 0.453491 8:-71518945 MsG0880046282.01.T01:CDS
GAGATGGAGATAAGAATTGA+TGG 0.457390 8:-71518378 MsG0880046282.01.T01:CDS
TCAATTCTTATCTCCATCTC+TGG 0.458329 8:+71518381 None:intergenic
GTGGAGGGATTTAGTGTAAG+TGG 0.468875 8:-71518135 MsG0880046282.01.T01:CDS
ACCATGTGATGGTTATTAAA+CGG 0.471768 8:+71518874 None:intergenic
GCTACATAGTATTCTGAAGT+TGG 0.475741 8:+71518654 None:intergenic
GGTGGTGATTCTGTTGTTGT+TGG 0.477307 8:-71518414 MsG0880046282.01.T01:CDS
GTAAGAAGAAATTCTCAACT+AGG 0.487655 8:-71518506 MsG0880046282.01.T01:CDS
GGACTATCAATATCAGTAGA+AGG 0.487808 8:-71518831 MsG0880046282.01.T01:CDS
AAGAATAGAGGAAATGGAAA+TGG 0.495722 8:-71518435 MsG0880046282.01.T01:CDS
AGGACGATGCAAGACTCAAT+TGG 0.498063 8:-71519014 None:intergenic
GGAGAAAACAAGGTTCAAAA+CGG 0.516137 8:-71518752 MsG0880046282.01.T01:CDS
CTCCATTATCAATTCAAGCA+AGG 0.540124 8:-71518170 MsG0880046282.01.T01:CDS
TAGGGCTAAGTTTCATGAGA+AGG 0.550186 8:-71518487 MsG0880046282.01.T01:CDS
TGGACCAGTTCAGCCAGAGA+TGG 0.550851 8:-71518394 MsG0880046282.01.T01:CDS
TAAGAAGAAATTCTCAACTA+GGG 0.554979 8:-71518505 MsG0880046282.01.T01:CDS
AATATAGGATCAATAAGAGC+TGG 0.556358 8:+71518555 None:intergenic
AGCTGTTGTTTGTGATGAAG+AGG 0.562228 8:-71518634 MsG0880046282.01.T01:CDS
AATAGAGGAAATGGAAATGG+TGG 0.567289 8:-71518432 MsG0880046282.01.T01:CDS
AGTTTCATGAGAAGGGTAAG+TGG 0.571065 8:-71518479 MsG0880046282.01.T01:CDS
AGGGCTAAGTTTCATGAGAA+GGG 0.578095 8:-71518486 MsG0880046282.01.T01:CDS
AGCAAGGAATTGTGAATCAG+TGG 0.580387 8:-71518154 MsG0880046282.01.T01:CDS
TGACATGTATACGCTCTGAC+CGG 0.584425 8:+71518926 None:intergenic
TCATCGGTTAGCTTCTCTGC+TGG 0.589818 8:+71518972 None:intergenic
CTTAAAGCTGCTAAATTCAA+CGG 0.595397 8:-71518687 MsG0880046282.01.T01:CDS
TTCATGTGAAGCATTTGCAA+TGG 0.606815 8:-71518344 MsG0880046282.01.T01:CDS
TCTGCTGGTGAAAAGCATGT+AGG 0.609956 8:+71518987 None:intergenic
CACACCGCCTCCGTGATCAT+CGG 0.616552 8:+71518956 None:intergenic
ATATCAGTAGAAGGTCCAGA+AGG 0.624892 8:-71518822 MsG0880046282.01.T01:CDS
AACAAAGCATGTTTCAAACC+AGG 0.625116 8:+71518240 None:intergenic
GAGAAGCTAACCGATGATCA+CGG 0.626304 8:-71518966 MsG0880046282.01.T01:CDS
AAGGAATTGTGAATCAGTGG+AGG 0.628434 8:-71518151 MsG0880046282.01.T01:CDS
CTAACCGATGATCACGGAGG+CGG 0.645937 8:-71518960 MsG0880046282.01.T01:CDS
AGGAATTGTGAATCAGTGGA+GGG 0.649065 8:-71518150 MsG0880046282.01.T01:CDS
GCAAGGTTGAACTAAAGCCA+TGG 0.651552 8:-71518782 MsG0880046282.01.T01:CDS
GCCGTTTAATAACCATCACA+TGG 0.661727 8:-71518875 MsG0880046282.01.T01:CDS
CATGTCAGTATACAGAACAA+AGG 0.665719 8:-71518910 MsG0880046282.01.T01:CDS
AGAAGCACAATATACCTGCA+AGG 0.665748 8:-71518799 MsG0880046282.01.T01:CDS
AAGCTAACCGATGATCACGG+AGG 0.775675 8:-71518963 MsG0880046282.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAGAATTGAAGTTTAT+TGG - Chr8:71518810-71518829 MsG0880046282.01.T01:CDS 20.0%
!! AATGAGAATTGAAGTTTATT+GGG - Chr8:71518811-71518830 MsG0880046282.01.T01:CDS 20.0%
! GAAATAGTAGAAGACAAAAT+TGG + Chr8:71518893-71518912 None:intergenic 25.0%
! TAAGAAGAAATTCTCAACTA+GGG - Chr8:71518592-71518611 MsG0880046282.01.T01:CDS 25.0%
!! TCAAAGAAAGAGCATATATT+TGG - Chr8:71518569-71518588 MsG0880046282.01.T01:CDS 25.0%
!!! TCTTTCTTTGAAACCAATAT+AGG + Chr8:71518560-71518579 None:intergenic 25.0%
!!! TGCAAAAATTTGTTACTTCA+TGG - Chr8:71518245-71518264 MsG0880046282.01.T01:CDS 25.0%
AAACATGCTTATAGAAGAAC+AGG - Chr8:71518512-71518531 MsG0880046282.01.T01:CDS 30.0%
AAGAATAGAGGAAATGGAAA+TGG - Chr8:71518662-71518681 MsG0880046282.01.T01:CDS 30.0%
AATATAGGATCAATAAGAGC+TGG + Chr8:71518545-71518564 None:intergenic 30.0%
ACCATGTGATGGTTATTAAA+CGG + Chr8:71518226-71518245 None:intergenic 30.0%
AGCTCTTATTGATCCTATAT+TGG - Chr8:71518544-71518563 MsG0880046282.01.T01:CDS 30.0%
CTTAAAGCTGCTAAATTCAA+CGG - Chr8:71518410-71518429 MsG0880046282.01.T01:CDS 30.0%
GTAAGAAGAAATTCTCAACT+AGG - Chr8:71518591-71518610 MsG0880046282.01.T01:CDS 30.0%
TCAAAACGGTTTATAGTTGA+TGG - Chr8:71518359-71518378 MsG0880046282.01.T01:CDS 30.0%
TCAATTGAATGCAAGAATAG+AGG - Chr8:71518650-71518669 MsG0880046282.01.T01:CDS 30.0%
TTCCTTGCTTGAATTGATAA+TGG + Chr8:71518932-71518951 None:intergenic 30.0%
TTTATTGGGATGTTCATGAT+TGG - Chr8:71518825-71518844 MsG0880046282.01.T01:CDS 30.0%
! AACTAAAGCCATGGTATTTT+TGG - Chr8:71518324-71518343 MsG0880046282.01.T01:CDS 30.0%
! GATAAGAATTGATGGACATT+TGG - Chr8:71518727-71518746 MsG0880046282.01.T01:CDS 30.0%
! TGTTTTCTCCAAAAATACCA+TGG + Chr8:71518335-71518354 None:intergenic 30.0%
! TTTGCTTGTTTCTTTATGCT+TGG - Chr8:71518996-71519015 MsG0880046282.01.T01:CDS 30.0%
!!! TGGTATTTTTGGAGAAAACA+AGG - Chr8:71518335-71518354 MsG0880046282.01.T01:CDS 30.0%
AACAAAGCATGTTTCAAACC+AGG + Chr8:71518860-71518879 None:intergenic 35.0%
AATAGAGGAAATGGAAATGG+TGG - Chr8:71518665-71518684 MsG0880046282.01.T01:CDS 35.0%
CATGTCAGTATACAGAACAA+AGG - Chr8:71518187-71518206 MsG0880046282.01.T01:CDS 35.0%
CATTTGCAATGGAAGTTTAG+AGG - Chr8:71518764-71518783 MsG0880046282.01.T01:CDS 35.0%
CGTTGAATTTAGCAGCTTTA+AGG + Chr8:71518412-71518431 None:intergenic 35.0%
CTCCATTATCAATTCAAGCA+AGG - Chr8:71518927-71518946 MsG0880046282.01.T01:CDS 35.0%
GAATGCAAGAATAGAGGAAA+TGG - Chr8:71518656-71518675 MsG0880046282.01.T01:CDS 35.0%
GAGATGGAGATAAGAATTGA+TGG - Chr8:71518719-71518738 MsG0880046282.01.T01:CDS 35.0%
GGACTATCAATATCAGTAGA+AGG - Chr8:71518266-71518285 MsG0880046282.01.T01:CDS 35.0%
GGAGAAAACAAGGTTCAAAA+CGG - Chr8:71518345-71518364 MsG0880046282.01.T01:CDS 35.0%
TCAATTCTTATCTCCATCTC+TGG + Chr8:71518719-71518738 None:intergenic 35.0%
! ACAAAGCTGTTGACATTTTC+TGG - Chr8:71518384-71518403 MsG0880046282.01.T01:CDS 35.0%
! CAAAGCTGTTGACATTTTCT+GGG - Chr8:71518385-71518404 MsG0880046282.01.T01:CDS 35.0%
! CAAATTTTTGCACCATGTGA+TGG + Chr8:71518237-71518256 None:intergenic 35.0%
! CATGATTGGTTATTTAGTCC+TGG - Chr8:71518839-71518858 MsG0880046282.01.T01:CDS 35.0%
! CTTGTTTCTTTATGCTTGGA+AGG - Chr8:71519000-71519019 MsG0880046282.01.T01:CDS 35.0%
! TTCATGTGAAGCATTTGCAA+TGG - Chr8:71518753-71518772 MsG0880046282.01.T01:CDS 35.0%
!! GCTACATAGTATTCTGAAGT+TGG + Chr8:71518446-71518465 None:intergenic 35.0%
AAGGAATTGTGAATCAGTGG+AGG - Chr8:71518946-71518965 MsG0880046282.01.T01:CDS 40.0%
AGCAAGGAATTGTGAATCAG+TGG - Chr8:71518943-71518962 MsG0880046282.01.T01:CDS 40.0%
AGGAATTGTGAATCAGTGGA+GGG - Chr8:71518947-71518966 MsG0880046282.01.T01:CDS 40.0%
AGGGCTAAGTTTCATGAGAA+GGG - Chr8:71518611-71518630 MsG0880046282.01.T01:CDS 40.0%
AGTTTCATGAGAAGGGTAAG+TGG - Chr8:71518618-71518637 MsG0880046282.01.T01:CDS 40.0%
ATATCAGTAGAAGGTCCAGA+AGG - Chr8:71518275-71518294 MsG0880046282.01.T01:CDS 40.0%
GAAGAGGTTGTTCTTCTTCT+TGG - Chr8:71518479-71518498 MsG0880046282.01.T01:CDS 40.0%
GCCGTTTAATAACCATCACA+TGG - Chr8:71518222-71518241 MsG0880046282.01.T01:CDS 40.0%
GTATATTGTGCTTCTCCTTC+TGG + Chr8:71518293-71518312 None:intergenic 40.0%
TAGGGCTAAGTTTCATGAGA+AGG - Chr8:71518610-71518629 MsG0880046282.01.T01:CDS 40.0%
! AGAAGCACAATATACCTGCA+AGG - Chr8:71518298-71518317 MsG0880046282.01.T01:CDS 40.0%
! AGCTGTTGTTTGTGATGAAG+AGG - Chr8:71518463-71518482 MsG0880046282.01.T01:CDS 40.0%
GAGAAGCTAACCGATGATCA+CGG - Chr8:71518131-71518150 MsG0880046282.01.T01:CDS 45.0%
GCAAGGTTGAACTAAAGCCA+TGG - Chr8:71518315-71518334 MsG0880046282.01.T01:CDS 45.0%
GGTGGTGATTCTGTTGTTGT+TGG - Chr8:71518683-71518702 MsG0880046282.01.T01:CDS 45.0%
GTGGAGGGATTTAGTGTAAG+TGG - Chr8:71518962-71518981 MsG0880046282.01.T01:CDS 45.0%
TGACATGTATACGCTCTGAC+CGG + Chr8:71518174-71518193 None:intergenic 45.0%
! TCTGCTGGTGAAAAGCATGT+AGG + Chr8:71518113-71518132 None:intergenic 45.0%
!! TGGCTTTAGTTCAACCTTGC+AGG + Chr8:71518315-71518334 None:intergenic 45.0%
AAGCTAACCGATGATCACGG+AGG - Chr8:71518134-71518153 MsG0880046282.01.T01:CDS 50.0%
ATCTCCATCTCTGGCTGAAC+TGG + Chr8:71518710-71518729 None:intergenic 50.0%
!! TCATCGGTTAGCTTCTCTGC+TGG + Chr8:71518128-71518147 None:intergenic 50.0%
CTAACCGATGATCACGGAGG+CGG - Chr8:71518137-71518156 MsG0880046282.01.T01:CDS 55.0%
TGGACCAGTTCAGCCAGAGA+TGG - Chr8:71518703-71518722 MsG0880046282.01.T01:CDS 55.0%
CACACCGCCTCCGTGATCAT+CGG + Chr8:71518144-71518163 None:intergenic 60.0%
GGAGGCGGTGTGACACGTTC+CGG - Chr8:71518152-71518171 MsG0880046282.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 71518089 71519030 71518089 ID=MsG0880046282.01;Name=MsG0880046282.01
Chr8 mRNA 71518089 71519030 71518089 ID=MsG0880046282.01.T01;Parent=MsG0880046282.01;Name=MsG0880046282.01.T01;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|313
Chr8 exon 71518089 71519030 71518089 ID=MsG0880046282.01.T01:exon:27854;Parent=MsG0880046282.01.T01
Chr8 CDS 71518089 71519030 71518089 ID=MsG0880046282.01.T01:cds;Parent=MsG0880046282.01.T01
Gene Sequence

>MsG0880046282.01.T01

ATGCAAGACTCAATTGGTATTCCTACATGCTTTTCACCAGCAGAGAAGCTAACCGATGATCACGGAGGCGGTGTGACACGTTCCGGTCAGAGCGTATACATGTCAGTATACAGAACAAAGGTAGCTGATCACTGCCGTTTAATAACCATCACATGGTGCAAAAATTTGTTACTTCATGGACTATCAATATCAGTAGAAGGTCCAGAAGGAGAAGCACAATATACCTGCAAGGTTGAACTAAAGCCATGGTATTTTTGGAGAAAACAAGGTTCAAAACGGTTTATAGTTGATGGAAACAAAGCTGTTGACATTTTCTGGGACCTTAAAGCTGCTAAATTCAACGGAGAAACAGAACCAACTTCAGAATACTATGTAGCTGTTGTTTGTGATGAAGAGGTTGTTCTTCTTCTTGGTGATCTCAAAAAACATGCTTATAGAAGAACAGGTTGTAGACCAGCTCTTATTGATCCTATATTGGTTTCAAAGAAAGAGCATATATTTGGTAAGAAGAAATTCTCAACTAGGGCTAAGTTTCATGAGAAGGGTAAGTGGCATGAGATTTCAATTGAATGCAAGAATAGAGGAAATGGAAATGGTGGTGATTCTGTTGTTGTTGGACCAGTTCAGCCAGAGATGGAGATAAGAATTGATGGACATTTGGTGATTCATGTGAAGCATTTGCAATGGAAGTTTAGAGGTAATGAATCAGTTCATCTTAATAAAATGAGAATTGAAGTTTATTGGGATGTTCATGATTGGTTATTTAGTCCTGGTTTGAAACATGCTTTGTTTATTTTTAAGCCAATTTTGTCTTCTACTATTTCATCTTCTTCATCTTCTCCATTATCAATTCAAGCAAGGAATTGTGAATCAGTGGAGGGATTTAGTGTAAGTGGTTCATCAGAGTTTTGCTTGTTTCTTTATGCTTGGAAGGTTGAATAA

Protein sequence

>MsG0880046282.01.T01

MQDSIGIPTCFSPAEKLTDDHGGGVTRSGQSVYMSVYRTKVADHCRLITITWCKNLLLHGLSISVEGPEGEAQYTCKVELKPWYFWRKQGSKRFIVDGNKAVDIFWDLKAAKFNGETEPTSEYYVAVVCDEEVVLLLGDLKKHAYRRTGCRPALIDPILVSKKEHIFGKKKFSTRAKFHEKGKWHEISIECKNRGNGNGGDSVVVGPVQPEMEIRIDGHLVIHVKHLQWKFRGNESVHLNKMRIEVYWDVHDWLFSPGLKHALFIFKPILSSTISSSSSSPLSIQARNCESVEGFSVSGSSEFCLFLYAWKVE*