AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035339.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035339.01.T01 MTR_6g081930 97.297 296 6 1 1 296 1 294 0.0 591
MsG0680035339.01.T01 MTR_8g106580 70.432 301 79 6 1 296 1 296 1.69e-128 367
MsG0680035339.01.T01 MTR_4g075730 45.846 325 133 9 1 295 6 317 6.69e-79 242
MsG0680035339.01.T01 MTR_2g101870 41.234 308 159 8 7 296 6 309 5.77e-69 216
MsG0680035339.01.T01 MTR_6g087880 38.730 315 165 7 1 296 1 306 9.04e-61 196
MsG0680035339.01.T01 MTR_0154s0010 56.296 135 59 0 162 296 1 135 3.90e-54 172
MsG0680035339.01.T01 MTR_4g075730 43.668 229 88 8 1 201 6 221 8.37e-45 152
MsG0680035339.01.T01 MTR_8g086610 37.857 280 146 8 23 295 20 278 6.57e-41 143
MsG0680035339.01.T01 MTR_3g037650 33.455 275 163 10 33 296 8 273 2.11e-40 142
MsG0680035339.01.T01 MTR_3g033530 25.604 207 131 6 89 278 129 329 2.46e-16 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035339.01.T01 AT1G45688 49.271 343 120 8 1 294 1 338 1.60e-100 298
MsG0680035339.01.T01 AT5G42860 51.084 323 122 10 1 294 1 316 3.40e-95 284
MsG0680035339.01.T01 AT1G45688 53.680 231 74 7 1 203 1 226 8.22e-75 229
MsG0680035339.01.T01 AT4G35170 39.799 299 154 7 18 294 3 297 3.28e-63 201
MsG0680035339.01.T01 AT2G41990 39.241 316 148 11 1 293 1 295 2.34e-52 174
MsG0680035339.01.T01 AT3G24605 34.386 285 166 7 1 274 3 277 2.88e-42 147
MsG0680035339.01.T01 AT3G24600 31.973 294 174 7 8 282 3 289 1.18e-33 125
MsG0680035339.01.T01 AT3G08490 22.628 274 185 7 30 293 11 267 4.71e-13 68.2

Find 82 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAACACTGGCACTTTCTT+TGG 0.173423 6:+102602663 MsG0680035339.01.T01:CDS
ATTAATTAATATAATCAAAA+TGG 0.180153 6:-102604050 None:intergenic
AGCTTATGTACTTGGAAATT+TGG 0.181196 6:+102603641 MsG0680035339.01.T01:CDS
TGAATATTCATATATGTTAA+AGG 0.229177 6:+102603762 MsG0680035339.01.T01:exon
TAATGGATTCCTTTGAGATA+TGG 0.280476 6:+102603846 MsG0680035339.01.T01:three_prime_UTR
ACTAGAGCACCAAGTTTCTT+AGG 0.281801 6:-102603711 None:intergenic
TGAAGCACAAATACTATAAA+AGG 0.290325 6:+102603664 MsG0680035339.01.T01:CDS
GAAAGTGTAAAAGAATTATT+AGG 0.332630 6:+102603799 MsG0680035339.01.T01:three_prime_UTR
AGAGGAATGGTGGTTATTCT+AGG 0.334315 6:+102601418 MsG0680035339.01.T01:CDS
TCAGATATAGTCATTGCTTC+AGG 0.340073 6:+102602723 MsG0680035339.01.T01:CDS
ACAAACCTTGATCACAATCT+TGG 0.367265 6:-102601532 None:intergenic
ACTCAGAAGGCAAGATGATT+AGG 0.375717 6:+102603896 MsG0680035339.01.T01:three_prime_UTR
GAATTTCAAAGTAGAGTTCA+TGG 0.389229 6:-102602634 None:intergenic
GAATATTCATATATGTTAAA+GGG 0.389673 6:+102603763 MsG0680035339.01.T01:exon
ATCACAATCTTGGGCTTCAT+AGG 0.399696 6:-102601522 None:intergenic
TTGATCTCATCACAAAGTTA+AGG 0.410482 6:-102603617 None:intergenic
TGAGATATGGATGGAATTGA+TGG 0.423689 6:+102603859 MsG0680035339.01.T01:three_prime_UTR
ACTTATGATATTATGATTTG+TGG 0.426768 6:-102600995 None:intergenic
GAAGCCCAAGATTGTGATCA+AGG 0.437262 6:+102601527 MsG0680035339.01.T01:CDS
ATTGATGTTATTGAAGAAGA+AGG 0.441499 6:+102601381 MsG0680035339.01.T01:CDS
GGTAGCAAGGTCCCTTTGTA+TGG 0.447350 6:+102603537 MsG0680035339.01.T01:CDS
CAAACCTTGATCACAATCTT+GGG 0.451461 6:-102601531 None:intergenic
CAAGCTGGTTCGGATTCGAC+TGG 0.458128 6:+102602591 MsG0680035339.01.T01:CDS
GCAAGCCTTAGCAGCACAAC+GGG 0.460597 6:+102603567 MsG0680035339.01.T01:CDS
TGTTACCGTCCAAGCTGGTT+CGG 0.463364 6:+102602581 MsG0680035339.01.T01:CDS
AGATCAAGAGCTTATGTACT+TGG 0.464263 6:+102603633 MsG0680035339.01.T01:CDS
TTAATTAATATAATCAAAAT+GGG 0.471258 6:-102604049 None:intergenic
ACCTTGGATCCTAAGAAACT+TGG 0.478203 6:+102603702 MsG0680035339.01.T01:CDS
ATCACAAAGTTAAGGTTCAA+TGG 0.479277 6:-102603609 None:intergenic
GAATGATGAGGTGAAGCAAC+AGG 0.483777 6:-102601279 None:intergenic
CCATGTTTCAGCTACACCCT+TGG 0.488475 6:+102602689 MsG0680035339.01.T01:CDS
TTGATGTTATTGAAGAAGAA+GGG 0.490404 6:+102601382 MsG0680035339.01.T01:CDS
ATCTAGGAAGAGTCATAGAT+TGG 0.493508 6:+102603497 MsG0680035339.01.T01:CDS
TTGGAGAAAGAGAGGAATGG+TGG 0.495776 6:+102601408 MsG0680035339.01.T01:CDS
GGACAAAGATCATGTTGGTT+CGG 0.497730 6:+102603948 MsG0680035339.01.T01:three_prime_UTR
AAGCTGGTTCGGATTCGACT+GGG 0.499334 6:+102602592 MsG0680035339.01.T01:CDS
TGAAGGAGTTTCATCAATCT+AGG 0.500553 6:+102603481 MsG0680035339.01.T01:CDS
ATTGGTGAGTGTGGCAGTGA+TGG 0.501754 6:+102603515 MsG0680035339.01.T01:CDS
ATAACATCAATATGCTTCCA+TGG 0.513867 6:-102601369 None:intergenic
GGACTTTGAACGTAGTATAG+TGG 0.522534 6:-102601210 None:intergenic
GCATACCCGTTGTGCTGCTA+AGG 0.522833 6:-102603572 None:intergenic
CGACGTGGTGGTGATCTTGT+TGG 0.523915 6:-102601186 None:intergenic
AAGTTAAGGTTCAATGGCAC+TGG 0.526221 6:-102603603 None:intergenic
CGAATCCGAACCAGCTTGGA+CGG 0.529659 6:-102602586 None:intergenic
TTGCACCACTTCCATACAAA+GGG 0.535212 6:-102603548 None:intergenic
ACCAAGTTTCTTAGGATCCA+AGG 0.536037 6:-102603703 None:intergenic
GTGAAGCAACAGGTGTTGAA+TGG 0.539497 6:-102601269 None:intergenic
GAACATGTTACCGTCCAAGC+TGG 0.543885 6:+102602576 MsG0680035339.01.T01:CDS
GATTCAATGTTCCATCACCT+TGG 0.548796 6:+102603686 MsG0680035339.01.T01:CDS
TTGGTGAGTGTGGCAGTGAT+GGG 0.550942 6:+102603516 MsG0680035339.01.T01:CDS
AAGGTCCCTTTGTATGGAAG+TGG 0.551894 6:+102603543 MsG0680035339.01.T01:CDS
AAATTCACATACCGCAACAC+TGG 0.552172 6:+102602651 MsG0680035339.01.T01:CDS
AGAATCATAGTGTTAATCCA+TGG 0.554930 6:+102601352 MsG0680035339.01.T01:CDS
GAGATATGGATGGAATTGAT+GGG 0.559554 6:+102603860 MsG0680035339.01.T01:three_prime_UTR
TTTCTTAGGATCCAAGGTGA+TGG 0.560780 6:-102603697 None:intergenic
CATATGTGCAAGAATTCTTG+AGG 0.561648 6:-102603737 None:intergenic
GTTCTTTGAATTATGCAATG+AGG 0.563916 6:+102603927 MsG0680035339.01.T01:three_prime_UTR
ATATGTGCAAGAATTCTTGA+GGG 0.565567 6:-102603736 None:intergenic
CAGTCGAATCCGAACCAGCT+TGG 0.565808 6:-102602590 None:intergenic
GGATTCCTTTGAGATATGGA+TGG 0.566300 6:+102603850 MsG0680035339.01.T01:three_prime_UTR
CCAAGGGTGTAGCTGAAACA+TGG 0.583937 6:-102602689 None:intergenic
ATCTGAATAAGACAGTTCCA+AGG 0.596282 6:-102602706 None:intergenic
GAGTCATAGATTGGTGAGTG+TGG 0.597085 6:+102603506 MsG0680035339.01.T01:CDS
TTCAATGGCACTGGAACTGT+AGG 0.597432 6:-102603594 None:intergenic
CAAAGAAAGTGCCAGTGTTG+CGG 0.604498 6:-102602662 None:intergenic
AGCAACAGGTGTTGAATGGA+AGG 0.610713 6:-102601265 None:intergenic
TCTGAATAAGACAGTTCCAA+GGG 0.610970 6:-102602705 None:intergenic
CAATTCCATCCATATCTCAA+AGG 0.611390 6:-102603855 None:intergenic
CTTGCACCACTTCCATACAA+AGG 0.614462 6:-102603549 None:intergenic
TAGTATAGTGGACGACGTGG+TGG 0.617154 6:-102601198 None:intergenic
TGTGGCAGTGATGGGTAGCA+AGG 0.618679 6:+102603524 MsG0680035339.01.T01:CDS
GATACAGCTCAGAACTCAGA+AGG 0.623173 6:+102603883 MsG0680035339.01.T01:three_prime_UTR
TGGTTCGGATTCGACTGGGG+TGG 0.641387 6:+102602596 MsG0680035339.01.T01:CDS
TGCAAGCCTTAGCAGCACAA+CGG 0.643607 6:+102603566 MsG0680035339.01.T01:CDS
AATGAGGACAAAGATCATGT+TGG 0.645906 6:+102603943 MsG0680035339.01.T01:three_prime_UTR
TGAATGGAAGGATGTAGTGA+CGG 0.653571 6:-102601253 None:intergenic
AGCTGGTTCGGATTCGACTG+GGG 0.661111 6:+102602593 MsG0680035339.01.T01:CDS
AGATATGGATGGAATTGATG+GGG 0.666943 6:+102603861 MsG0680035339.01.T01:three_prime_UTR
GTTCATGGTGATCATATCAG+TGG 0.667894 6:-102602619 None:intergenic
GTGGAAGAAGAAGAATGATG+AGG 0.698905 6:-102601291 None:intergenic
ACGTAGTATAGTGGACGACG+TGG 0.744646 6:-102601201 None:intergenic
GTTCAAAGTCCATCACACGA+CGG 0.801345 6:+102601222 MsG0680035339.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTATTTTTTTTATTAA+TGG + Chr6:102603829-102603848 MsG0680035339.01.T01:three_prime_UTR 0.0%
!!! AAAATTAATTTTGATTAGAT+TGG + Chr6:102603079-102603098 MsG0680035339.01.T01:intron 10.0%
!!! ATGATAAATTTTTCAATTTT+AGG + Chr6:102601731-102601750 MsG0680035339.01.T01:intron 10.0%
!! GAATATTCATATATGTTAAA+GGG + Chr6:102603763-102603782 MsG0680035339.01.T01:exon 15.0%
!! TGAATATTCATATATGTTAA+AGG + Chr6:102603762-102603781 MsG0680035339.01.T01:exon 15.0%
!!! TTTATTGATTTTGTGATTTT+AGG + Chr6:102601647-102601666 MsG0680035339.01.T01:intron 15.0%
!!! TTTTGATTATTGTTTTTTGA+GGG + Chr6:102601556-102601575 MsG0680035339.01.T01:intron 15.0%
!! AGAAATAGAAGAAAAAACAT+AGG + Chr6:102601055-102601074 MsG0680035339.01.T01:five_prime_UTR 20.0%
!! ATGTGTTAGAAAAATACAAA+AGG - Chr6:102603448-102603467 None:intergenic 20.0%
!! ATTAAGAAGTTTCAATTTGT+AGG - Chr6:102602968-102602987 None:intergenic 20.0%
!! TGTGTTAGAAAAATACAAAA+GGG - Chr6:102603447-102603466 None:intergenic 20.0%
!!! AAGATCAATTTTGCATTTTT+AGG + Chr6:102601982-102602001 MsG0680035339.01.T01:intron 20.0%
!!! AGATCAATTTTGCATTTTTA+AGG + Chr6:102601816-102601835 MsG0680035339.01.T01:intron 20.0%
!!! AGATCAATTTTGCATTTTTA+GGG + Chr6:102601816-102601835 MsG0680035339.01.T01:intron 20.0%
!!! ATGATCAAATTTGGATTTTT+AGG + Chr6:102602117-102602136 MsG0680035339.01.T01:intron 20.0%
!!! ATGTTCAATTTTGCATTTTA+GGG + Chr6:102601773-102601792 MsG0680035339.01.T01:intron 20.0%
!!! CAAAAATCAATTTTAAGTCA+TGG + Chr6:102603266-102603285 MsG0680035339.01.T01:intron 20.0%
!!! GAAAGTGTAAAAGAATTATT+AGG + Chr6:102603799-102603818 MsG0680035339.01.T01:three_prime_UTR 20.0%
!!! GATCAATTTTGCATTTTTAA+GGG + Chr6:102601817-102601836 MsG0680035339.01.T01:intron 20.0%
!!! GTAGTTAAGAAAATTGATTT+TGG + Chr6:102602781-102602800 MsG0680035339.01.T01:intron 20.0%
!!! GTGATAAATTTTGCTTTTTT+AGG + Chr6:102601689-102601708 MsG0680035339.01.T01:intron 20.0%
!!! GTTTTGATTATTGTTTTTTG+AGG + Chr6:102601555-102601574 MsG0680035339.01.T01:intron 20.0%
!!! TATCCATTTTGCATTATTTT+AGG + Chr6:102602445-102602464 MsG0680035339.01.T01:intron 20.0%
!!! TTTAAGTTGAAATGTAAACT+GGG + Chr6:102601917-102601936 MsG0680035339.01.T01:intron 20.0%
!!! TTTAGGTTGAAATTTGAATT+GGG + Chr6:102602051-102602070 MsG0680035339.01.T01:intron 20.0%
!!! TTTTAAGTTGAAATGTAAAC+TGG + Chr6:102601916-102601935 MsG0680035339.01.T01:intron 20.0%
!!! TTTTAGGTTGAAATTTGAAT+TGG + Chr6:102602050-102602069 MsG0680035339.01.T01:intron 20.0%
! AATGGAAACTTATCAAAAAC+CGG - Chr6:102602399-102602418 None:intergenic 25.0%
! ATAAAAAATTGAACATGTCC+CGG - Chr6:102602486-102602505 None:intergenic 25.0%
! ATTGATGTTATTGAAGAAGA+AGG + Chr6:102601381-102601400 MsG0680035339.01.T01:CDS 25.0%
! CAAGTTATAATTCATTCTAG+AGG + Chr6:102602911-102602930 MsG0680035339.01.T01:intron 25.0%
! CATCCTAAAATAATGCAAAA+TGG - Chr6:102602451-102602470 None:intergenic 25.0%
! GTTCAGATTCATTATAGAAA+TGG - Chr6:102602417-102602436 None:intergenic 25.0%
! TCAACCTGAAAATGAAAAAT+TGG - Chr6:102602368-102602387 None:intergenic 25.0%
! TGAAAACTTGAAATCTGAAT+GGG + Chr6:102602007-102602026 MsG0680035339.01.T01:intron 25.0%
! TGAAGCACAAATACTATAAA+AGG + Chr6:102603664-102603683 MsG0680035339.01.T01:CDS 25.0%
! TGTAGAATGAAATTTGAACT+GGG + Chr6:102602092-102602111 MsG0680035339.01.T01:intron 25.0%
! TTGAAAACTTGAAATCTGAA+TGG + Chr6:102602006-102602025 MsG0680035339.01.T01:intron 25.0%
! TTGAGTGTATAAATCATGTT+TGG + Chr6:102603180-102603199 MsG0680035339.01.T01:intron 25.0%
! TTGATGTTATTGAAGAAGAA+GGG + Chr6:102601382-102601401 MsG0680035339.01.T01:CDS 25.0%
! TTGTAGAATGAAATTTGAAC+TGG + Chr6:102602091-102602110 MsG0680035339.01.T01:intron 25.0%
!! CATGTTCAATTTTGCATTTT+AGG + Chr6:102601772-102601791 MsG0680035339.01.T01:intron 25.0%
!! TTTAAGATGATGTTTGAACT+GGG + Chr6:102601875-102601894 MsG0680035339.01.T01:intron 25.0%
!! TTTAGGATGAAATTTGAACT+GGG + Chr6:102601706-102601725 MsG0680035339.01.T01:intron 25.0%
!! TTTGATTGATTGTTCAGTTT+TGG + Chr6:102601604-102601623 MsG0680035339.01.T01:intron 25.0%
!! TTTGGAATGTGATTTGAATT+GGG + Chr6:102601622-102601641 MsG0680035339.01.T01:intron 25.0%
!!! ATTGATTTTTGTATGTTCGA+TGG - Chr6:102603257-102603276 None:intergenic 25.0%
!!! ATTTTTAAGATGATCTGAAC+GGG + Chr6:102601956-102601975 MsG0680035339.01.T01:intron 25.0%
!!! CATTTTTAAGATGATCTGAA+CGG + Chr6:102601955-102601974 MsG0680035339.01.T01:intron 25.0%
!!! TATTGTTTTTTGAGGGTTTT+TGG + Chr6:102601563-102601582 MsG0680035339.01.T01:intron 25.0%
!!! TCAATTTTGCATTTTTAGAG+TGG + Chr6:102602230-102602249 MsG0680035339.01.T01:intron 25.0%
!!! TTAAGGGTGAAATTTTAACT+GGG + Chr6:102601833-102601852 MsG0680035339.01.T01:intron 25.0%
!!! TTTAAGGGTGAAATTTTAAC+TGG + Chr6:102601832-102601851 MsG0680035339.01.T01:intron 25.0%
!!! TTTAGGTTGAAATGTAAACT+GGG + Chr6:102601748-102601767 MsG0680035339.01.T01:intron 25.0%
!!! TTTGACTTGAAATCTTAACT+GGG + Chr6:102602286-102602305 MsG0680035339.01.T01:intron 25.0%
!!! TTTGTTTTCGCTTATTCTTT+GGG + Chr6:102601488-102601507 MsG0680035339.01.T01:CDS 25.0%
!!! TTTTAAGATGATGTTTGAAC+TGG + Chr6:102601874-102601893 MsG0680035339.01.T01:intron 25.0%
!!! TTTTAGGATGAAATTTGAAC+TGG + Chr6:102601705-102601724 MsG0680035339.01.T01:intron 25.0%
!!! TTTTAGGATGTGATTTGAAT+TGG + Chr6:102601663-102601682 MsG0680035339.01.T01:intron 25.0%
!!! TTTTAGGTTGAAATGTAAAC+TGG + Chr6:102601747-102601766 MsG0680035339.01.T01:intron 25.0%
!!! TTTTGACTTGAAATCTTAAC+TGG + Chr6:102602285-102602304 MsG0680035339.01.T01:intron 25.0%
!!! TTTTGGAATGTGATTTGAAT+TGG + Chr6:102601621-102601640 MsG0680035339.01.T01:intron 25.0%
AAACATTGTGATTGTGTGTT+TGG + Chr6:102602937-102602956 MsG0680035339.01.T01:intron 30.0%
ACCAACTATATATGCATGTT+TGG - Chr6:102603388-102603407 None:intergenic 30.0%
AGAATCATAGTGTTAATCCA+TGG + Chr6:102601352-102601371 MsG0680035339.01.T01:CDS 30.0%
AGCTTATGTACTTGGAAATT+TGG + Chr6:102603641-102603660 MsG0680035339.01.T01:CDS 30.0%
ATAACATCAATATGCTTCCA+TGG - Chr6:102601372-102601391 None:intergenic 30.0%
ATATGTGCAAGAATTCTTGA+GGG - Chr6:102603739-102603758 None:intergenic 30.0%
ATCACAAAGTTAAGGTTCAA+TGG - Chr6:102603612-102603631 None:intergenic 30.0%
GAATTTCAAAGTAGAGTTCA+TGG - Chr6:102602637-102602656 None:intergenic 30.0%
GATTCACTAACTCATTTGTT+TGG + Chr6:102602754-102602773 MsG0680035339.01.T01:intron 30.0%
GTTCTTTGAATTATGCAATG+AGG + Chr6:102603927-102603946 MsG0680035339.01.T01:three_prime_UTR 30.0%
TAGGATGATGAAATCTTAAC+CGG + Chr6:102602464-102602483 MsG0680035339.01.T01:intron 30.0%
TCAACCTAAAAGTGAAACAT+TGG - Chr6:102602041-102602060 None:intergenic 30.0%
TCCAAACATGCATATATAGT+TGG + Chr6:102603384-102603403 MsG0680035339.01.T01:intron 30.0%
TTAGGGTTGAAATATGAACT+GGG + Chr6:102601790-102601809 MsG0680035339.01.T01:intron 30.0%
TTGATCTCATCACAAAGTTA+AGG - Chr6:102603620-102603639 None:intergenic 30.0%
TTTCACAGTGAGTTTACATT+GGG + Chr6:102602988-102603007 MsG0680035339.01.T01:intron 30.0%
! ACAAACACTAAGCATGTTTT+TGG - Chr6:102603042-102603061 None:intergenic 30.0%
! ACATTGATTATTGCAGATGA+AGG + Chr6:102603464-102603483 MsG0680035339.01.T01:intron 30.0%
! ATGAACAGCTTGGATTTTTA+GGG + Chr6:102602318-102602337 MsG0680035339.01.T01:intron 30.0%
! GAAGCTGAGATTTGTAATTT+TGG - Chr6:102602892-102602911 None:intergenic 30.0%
! TATTGAAGAAGAAGGGTTTT+TGG + Chr6:102601389-102601408 MsG0680035339.01.T01:CDS 30.0%
! TGAGTTGAACAATAAATGTG+AGG + Chr6:102602858-102602877 MsG0680035339.01.T01:intron 30.0%
! TGCTCCAATTTTTCATTTTC+AGG + Chr6:102602361-102602380 MsG0680035339.01.T01:intron 30.0%
! TTGTTTTCGCTTATTCTTTG+GGG + Chr6:102601489-102601508 MsG0680035339.01.T01:CDS 30.0%
! TTTAGGGTTGAAATATGAAC+TGG + Chr6:102601789-102601808 MsG0680035339.01.T01:intron 30.0%
! TTTAGGGTTGAAATCTGATT+GGG + Chr6:102602334-102602353 MsG0680035339.01.T01:intron 30.0%
! TTTAGGTTTGAAACGTAAAC+TGG + Chr6:102602134-102602153 MsG0680035339.01.T01:intron 30.0%
! TTTTCACAGTGAGTTTACAT+TGG + Chr6:102602987-102603006 MsG0680035339.01.T01:intron 30.0%
! TTTTGGATGAAATTGGAACT+GGG + Chr6:102601580-102601599 MsG0680035339.01.T01:intron 30.0%
! TTTTTGGATGAAATTGGAAC+TGG + Chr6:102601579-102601598 MsG0680035339.01.T01:intron 30.0%
!! GAATTGATTGACTCTAGAAT+TGG - Chr6:102603224-102603243 None:intergenic 30.0%
!! TAATGGATTCCTTTGAGATA+TGG + Chr6:102603846-102603865 MsG0680035339.01.T01:three_prime_UTR 30.0%
!!! ATTTTCTGGCTTTTGTTGTT+GGG + Chr6:102601448-102601467 MsG0680035339.01.T01:CDS 30.0%
!!! CTTTGTTTTCGCTTATTCTT+TGG + Chr6:102601487-102601506 MsG0680035339.01.T01:CDS 30.0%
!!! TATTTTCTGGCTTTTGTTGT+TGG + Chr6:102601447-102601466 MsG0680035339.01.T01:CDS 30.0%
!!! TTCTAGGAAGTGTTATTTTC+TGG + Chr6:102601434-102601453 MsG0680035339.01.T01:CDS 30.0%
!!! TTTTAGGGTTGAAATCTGAT+TGG + Chr6:102602333-102602352 MsG0680035339.01.T01:intron 30.0%
!!! TTTTTAAGATGATCTGAACG+GGG + Chr6:102601957-102601976 MsG0680035339.01.T01:intron 30.0%
AACTGGGTCATGATCAAATT+TGG + Chr6:102602108-102602127 MsG0680035339.01.T01:intron 35.0%
AATGAGGACAAAGATCATGT+TGG + Chr6:102603943-102603962 MsG0680035339.01.T01:three_prime_UTR 35.0%
ACAAACCTTGATCACAATCT+TGG - Chr6:102601535-102601554 None:intergenic 35.0%
AGATATGGATGGAATTGATG+GGG + Chr6:102603861-102603880 MsG0680035339.01.T01:three_prime_UTR 35.0%
AGATCAAGAGCTTATGTACT+TGG + Chr6:102603633-102603652 MsG0680035339.01.T01:CDS 35.0%
AGGATGATGAAATCTTAACC+GGG + Chr6:102602465-102602484 MsG0680035339.01.T01:intron 35.0%
ATCTAGGAAGAGTCATAGAT+TGG + Chr6:102603497-102603516 MsG0680035339.01.T01:CDS 35.0%
ATCTGAATAAGACAGTTCCA+AGG - Chr6:102602709-102602728 None:intergenic 35.0%
CAAACCTTGATCACAATCTT+GGG - Chr6:102601534-102601553 None:intergenic 35.0%
CAATTCCATCCATATCTCAA+AGG - Chr6:102603858-102603877 None:intergenic 35.0%
CATATGTGCAAGAATTCTTG+AGG - Chr6:102603740-102603759 None:intergenic 35.0%
CGTGTGTTGAAATTTGAACT+GGG + Chr6:102602159-102602178 MsG0680035339.01.T01:intron 35.0%
CTTAGAAGCAGAAAAATGAG+TGG - Chr6:102601313-102601332 None:intergenic 35.0%
GAGATATGGATGGAATTGAT+GGG + Chr6:102603860-102603879 MsG0680035339.01.T01:three_prime_UTR 35.0%
TCAGATATAGTCATTGCTTC+AGG + Chr6:102602723-102602742 MsG0680035339.01.T01:CDS 35.0%
TCGTGTGTTGAAATTTGAAC+TGG + Chr6:102602158-102602177 MsG0680035339.01.T01:intron 35.0%
TCTGAATAAGACAGTTCCAA+GGG - Chr6:102602708-102602727 None:intergenic 35.0%
TGAAGGAGTTTCATCAATCT+AGG + Chr6:102603481-102603500 MsG0680035339.01.T01:CDS 35.0%
TGAGATATGGATGGAATTGA+TGG + Chr6:102603859-102603878 MsG0680035339.01.T01:three_prime_UTR 35.0%
! CATGAACAGCTTGGATTTTT+AGG + Chr6:102602317-102602336 MsG0680035339.01.T01:intron 35.0%
!! GTGTTTGTTTGTTTCTACTG+TGG + Chr6:102603054-102603073 MsG0680035339.01.T01:intron 35.0%
!! TGAGGGTTTTTGGATGAAAT+TGG + Chr6:102601573-102601592 MsG0680035339.01.T01:intron 35.0%
!! TGCTCCAATGTTTCACTTTT+AGG + Chr6:102602034-102602053 MsG0680035339.01.T01:intron 35.0%
!! TTTCAGGTTGAAATCTGAAC+CGG + Chr6:102602377-102602396 MsG0680035339.01.T01:intron 35.0%
!! TTTTTGGAGAAAGAGAGGAA+TGG + Chr6:102601405-102601424 MsG0680035339.01.T01:CDS 35.0%
AAATTCACATACCGCAACAC+TGG + Chr6:102602651-102602670 MsG0680035339.01.T01:CDS 40.0%
AAGTTAAGGTTCAATGGCAC+TGG - Chr6:102603606-102603625 None:intergenic 40.0%
ACCAAGTTTCTTAGGATCCA+AGG - Chr6:102603706-102603725 None:intergenic 40.0%
ACCTTGGATCCTAAGAAACT+TGG + Chr6:102603702-102603721 MsG0680035339.01.T01:CDS 40.0%
ACTCAGAAGGCAAGATGATT+AGG + Chr6:102603896-102603915 MsG0680035339.01.T01:three_prime_UTR 40.0%
AGAGGAATGGTGGTTATTCT+AGG + Chr6:102601418-102601437 MsG0680035339.01.T01:CDS 40.0%
ATCACAATCTTGGGCTTCAT+AGG - Chr6:102601525-102601544 None:intergenic 40.0%
GATTCAATGTTCCATCACCT+TGG + Chr6:102603686-102603705 MsG0680035339.01.T01:CDS 40.0%
GGACTTTGAACGTAGTATAG+TGG - Chr6:102601213-102601232 None:intergenic 40.0%
GTGGAAGAAGAAGAATGATG+AGG - Chr6:102601294-102601313 None:intergenic 40.0%
GTTCATGGTGATCATATCAG+TGG - Chr6:102602622-102602641 None:intergenic 40.0%
TGAATGGAAGGATGTAGTGA+CGG - Chr6:102601256-102601275 None:intergenic 40.0%
TTGCACCACTTCCATACAAA+GGG - Chr6:102603551-102603570 None:intergenic 40.0%
TTTCTTAGGATCCAAGGTGA+TGG - Chr6:102603700-102603719 None:intergenic 40.0%
! ACTAGAGCACCAAGTTTCTT+AGG - Chr6:102603714-102603733 None:intergenic 40.0%
! GGACAAAGATCATGTTGGTT+CGG + Chr6:102603948-102603967 MsG0680035339.01.T01:three_prime_UTR 40.0%
!! AAGGGTTTTTGGAGAAAGAG+AGG + Chr6:102601400-102601419 MsG0680035339.01.T01:CDS 40.0%
!! GGATTCCTTTGAGATATGGA+TGG + Chr6:102603850-102603869 MsG0680035339.01.T01:three_prime_UTR 40.0%
!! TGTTTGGATTCGCTTTTGTG+AGG - Chr6:102603372-102603391 None:intergenic 40.0%
!!! CACTAAGCATGTTTTTGGTG+TGG - Chr6:102603037-102603056 None:intergenic 40.0%
!!! TAAGCATGTTTTTGGTGTGG+TGG - Chr6:102603034-102603053 None:intergenic 40.0%
AAGGTCCCTTTGTATGGAAG+TGG + Chr6:102603543-102603562 MsG0680035339.01.T01:CDS 45.0%
CACAGTGAGTTTACATTGGG+AGG + Chr6:102602991-102603010 MsG0680035339.01.T01:intron 45.0%
CTTGCACCACTTCCATACAA+AGG - Chr6:102603552-102603571 None:intergenic 45.0%
GAAGCCCAAGATTGTGATCA+AGG + Chr6:102601527-102601546 MsG0680035339.01.T01:CDS 45.0%
GAATGATGAGGTGAAGCAAC+AGG - Chr6:102601282-102601301 None:intergenic 45.0%
GAGTCATAGATTGGTGAGTG+TGG + Chr6:102603506-102603525 MsG0680035339.01.T01:CDS 45.0%
GATACAGCTCAGAACTCAGA+AGG + Chr6:102603883-102603902 MsG0680035339.01.T01:three_prime_UTR 45.0%
GGGTTGAAATCTGATTGGGT+TGG + Chr6:102602338-102602357 MsG0680035339.01.T01:intron 45.0%
GTCATGACTCATGAACAGCT+TGG + Chr6:102602308-102602327 MsG0680035339.01.T01:intron 45.0%
GTTCAAAGTCCATCACACGA+CGG + Chr6:102601222-102601241 MsG0680035339.01.T01:CDS 45.0%
TTGGAGAAAGAGAGGAATGG+TGG + Chr6:102601408-102601427 MsG0680035339.01.T01:CDS 45.0%
! TTCAATGGCACTGGAACTGT+AGG - Chr6:102603597-102603616 None:intergenic 45.0%
!! AGCAACAGGTGTTGAATGGA+AGG - Chr6:102601268-102601287 None:intergenic 45.0%
!! CAAAGAAAGTGCCAGTGTTG+CGG - Chr6:102602665-102602684 None:intergenic 45.0%
!! GTGAAGCAACAGGTGTTGAA+TGG - Chr6:102601272-102601291 None:intergenic 45.0%
!!! GTTTTTTCACCGTCGTGTGA+TGG - Chr6:102601234-102601253 None:intergenic 45.0%
!! ATTAATTAATATAATCAAAA+TGG - Chr6:102604053-102604072 None:intergenic 5.0%
!! TTAATTAATATAATCAAAAT+GGG - Chr6:102604052-102604071 None:intergenic 5.0%
ATTGGTGAGTGTGGCAGTGA+TGG + Chr6:102603515-102603534 MsG0680035339.01.T01:CDS 50.0%
CCATGTTTCAGCTACACCCT+TGG + Chr6:102602689-102602708 MsG0680035339.01.T01:CDS 50.0%
CGCAACACTGGCACTTTCTT+TGG + Chr6:102602663-102602682 MsG0680035339.01.T01:CDS 50.0%
GAACATGTTACCGTCCAAGC+TGG + Chr6:102602576-102602595 MsG0680035339.01.T01:CDS 50.0%
GGTAGCAAGGTCCCTTTGTA+TGG + Chr6:102603537-102603556 MsG0680035339.01.T01:CDS 50.0%
TGCAAGCCTTAGCAGCACAA+CGG + Chr6:102603566-102603585 MsG0680035339.01.T01:CDS 50.0%
TGTTACCGTCCAAGCTGGTT+CGG + Chr6:102602581-102602600 MsG0680035339.01.T01:CDS 50.0%
TTGGTGAGTGTGGCAGTGAT+GGG + Chr6:102603516-102603535 MsG0680035339.01.T01:CDS 50.0%
! CCAAGGGTGTAGCTGAAACA+TGG - Chr6:102602692-102602711 None:intergenic 50.0%
!! AAGCTGGTTCGGATTCGACT+GGG + Chr6:102602592-102602611 MsG0680035339.01.T01:CDS 50.0%
!! ACGTAGTATAGTGGACGACG+TGG - Chr6:102601204-102601223 None:intergenic 50.0%
!! TAGTATAGTGGACGACGTGG+TGG - Chr6:102601201-102601220 None:intergenic 50.0%
CAGTCGAATCCGAACCAGCT+TGG - Chr6:102602593-102602612 None:intergenic 55.0%
CGAATCCGAACCAGCTTGGA+CGG - Chr6:102602589-102602608 None:intergenic 55.0%
GCAAGCCTTAGCAGCACAAC+GGG + Chr6:102603567-102603586 MsG0680035339.01.T01:CDS 55.0%
! CGACGTGGTGGTGATCTTGT+TGG - Chr6:102601189-102601208 None:intergenic 55.0%
! GCATACCCGTTGTGCTGCTA+AGG - Chr6:102603575-102603594 None:intergenic 55.0%
!! AGCTGGTTCGGATTCGACTG+GGG + Chr6:102602593-102602612 MsG0680035339.01.T01:CDS 55.0%
!! CAAGCTGGTTCGGATTCGAC+TGG + Chr6:102602591-102602610 MsG0680035339.01.T01:CDS 55.0%
!! TGTGGCAGTGATGGGTAGCA+AGG + Chr6:102603524-102603543 MsG0680035339.01.T01:CDS 55.0%
! TGGTTCGGATTCGACTGGGG+TGG + Chr6:102602596-102602615 MsG0680035339.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 102600997 102604082 102600997 ID=MsG0680035339.01;Name=MsG0680035339.01
Chr6 mRNA 102600997 102604082 102600997 ID=MsG0680035339.01.T01;Parent=MsG0680035339.01;Name=MsG0680035339.01.T01;_AED=0.31;_eAED=0.31;_QI=132|1|1|1|1|1|3|318|296
Chr6 exon 102600997 102601548 102600997 ID=MsG0680035339.01.T01:exon:10940;Parent=MsG0680035339.01.T01
Chr6 exon 102602564 102602749 102602564 ID=MsG0680035339.01.T01:exon:10941;Parent=MsG0680035339.01.T01
Chr6 exon 102603480 102604082 102603480 ID=MsG0680035339.01.T01:exon:10942;Parent=MsG0680035339.01.T01
Chr6 five_prime_UTR 102600997 102601128 102600997 ID=MsG0680035339.01.T01:five_prime_utr;Parent=MsG0680035339.01.T01
Chr6 CDS 102601129 102601548 102601129 ID=MsG0680035339.01.T01:cds;Parent=MsG0680035339.01.T01
Chr6 CDS 102602564 102602749 102602564 ID=MsG0680035339.01.T01:cds;Parent=MsG0680035339.01.T01
Chr6 CDS 102603480 102603764 102603480 ID=MsG0680035339.01.T01:cds;Parent=MsG0680035339.01.T01
Chr6 three_prime_UTR 102603765 102604082 102603765 ID=MsG0680035339.01.T01:three_prime_utr;Parent=MsG0680035339.01.T01
Gene Sequence

>MsG0680035339.01.T01

ATGCATACAAAAACAGATTCAGAAGTTACAACAACAAGTGTAGCAACATCAACATCACCAACAAGATCACCACCACGTCGTCCACTATACTACGTTCAAAGTCCATCACACGACGGTGAAAAAACCGTCACTACATCCTTCCATTCAACACCTGTTGCTTCACCTCATCATTCTTCTTCTTCCACTCATTTTTCTGCTTCTAAGAAAGAACAACAACATCAACAGAATCATAGTGTTAATCCATGGAAGCATATTGATGTTATTGAAGAAGAAGGGTTTTTGGAGAAAGAGAGGAATGGTGGTTATTCTAGGAAGTGTTATTTTCTGGCTTTTGTTGTTGGGTTCTTTGTTTTGTTCTCTTTGTTTTCGCTTATTCTTTGGGGTGCTAGTAGACCTATGAAGCCCAAGATTGTGATCAAGAGCATAAAGTTTGAACATGTTACCGTCCAAGCTGGTTCGGATTCGACTGGGGTGGCCACTGATATGATCACCATGAACTCTACTTTGAAATTCACATACCGCAACACTGGCACTTTCTTTGGAGTCCATGTTTCAGCTACACCCTTGGAACTGTCTTATTCAGATATAGTCATTGCTTCAGGAAATATGAAGGAGTTTCATCAATCTAGGAAGAGTCATAGATTGGTGAGTGTGGCAGTGATGGGTAGCAAGGTCCCTTTGTATGGAAGTGGTGCAAGCCTTAGCAGCACAACGGGTATGCCTACAGTTCCAGTGCCATTGAACCTTAACTTTGTGATGAGATCAAGAGCTTATGTACTTGGAAATTTGGTGAAGCACAAATACTATAAAAGGATTCAATGTTCCATCACCTTGGATCCTAAGAAACTTGGTGCTCTAGTTTCCCTCAAGAATTCTTGCACATATGATTGA
Protein sequence

>MsG0680035339.01.T01

MHTKTDSEVTTTSVATSTSPTRSPPRRPLYYVQSPSHDGEKTVTTSFHSTPVASPHHSSSSTHFSASKKEQQHQQNHSVNPWKHIDVIEEEGFLEKERNGGYSRKCYFLAFVVGFFVLFSLFSLILWGASRPMKPKIVIKSIKFEHVTVQAGSDSTGVATDMITMNSTLKFTYRNTGTFFGVHVSATPLELSYSDIVIASGNMKEFHQSRKSHRLVSVAVMGSKVPLYGSGASLSSTTGMPTVPVPLNLNFVMRSRAYVLGNLVKHKYYKRIQCSITLDPKKLGALVSLKNSCTYD*