Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046415.01.T01 | XP_039689437.1 | 96.694 | 121 | 4 | 0 | 21 | 141 | 164 | 284 | 1.11E-75 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046415.01.T01 | Q9FM19 | 87.395 | 119 | 15 | 0 | 21 | 139 | 164 | 282 | 8.83E-71 | 217 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046415.01.T01 | B7FKT5 | 96.694 | 121 | 4 | 0 | 21 | 141 | 164 | 284 | 5.30e-76 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480023819.01 | MsG0880046415.01 | 0.813764 | 2.169787e-51 | 1.429492e-48 |
| MsG0280010244.01 | MsG0880046415.01 | 0.800967 | 1.124215e-48 | 5.305612e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046415.01.T01 | MTR_4g107730 | 96.694 | 121 | 4 | 0 | 21 | 141 | 162 | 282 | 1.47e-79 | 236 |
| MsG0880046415.01.T01 | MTR_4g107830 | 87.603 | 121 | 15 | 0 | 21 | 141 | 179 | 299 | 1.43e-71 | 216 |
| MsG0880046415.01.T01 | MTR_6g011380 | 82.353 | 119 | 21 | 0 | 21 | 139 | 164 | 282 | 6.60e-68 | 207 |
| MsG0880046415.01.T01 | MTR_4g107720 | 90.083 | 121 | 12 | 0 | 21 | 141 | 164 | 284 | 1.07e-66 | 204 |
| MsG0880046415.01.T01 | MTR_4g107810 | 90.756 | 119 | 11 | 0 | 21 | 139 | 164 | 282 | 1.76e-65 | 201 |
| MsG0880046415.01.T01 | MTR_4g107760 | 90.756 | 119 | 11 | 0 | 21 | 139 | 164 | 282 | 1.76e-65 | 201 |
| MsG0880046415.01.T01 | MTR_4g107810 | 90.756 | 119 | 11 | 0 | 21 | 139 | 224 | 342 | 7.79e-65 | 201 |
| MsG0880046415.01.T01 | MTR_8g469160 | 74.790 | 119 | 30 | 0 | 21 | 139 | 164 | 282 | 2.66e-62 | 192 |
| MsG0880046415.01.T01 | MTR_4g107700 | 92.771 | 83 | 6 | 0 | 59 | 141 | 6 | 88 | 5.42e-52 | 160 |
| MsG0880046415.01.T01 | MTR_6g460470 | 57.983 | 119 | 50 | 0 | 21 | 139 | 166 | 284 | 7.75e-47 | 153 |
| MsG0880046415.01.T01 | MTR_6g060500 | 57.447 | 47 | 20 | 0 | 23 | 69 | 19 | 65 | 2.72e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046415.01.T01 | AT5G62740 | 87.395 | 119 | 15 | 0 | 21 | 139 | 164 | 282 | 9.00e-72 | 217 |
| MsG0880046415.01.T01 | AT3G01290 | 78.333 | 120 | 26 | 0 | 21 | 140 | 164 | 283 | 2.53e-65 | 200 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT1G69840 | 81.513 | 119 | 22 | 0 | 21 | 139 | 164 | 282 | 6.40e-60 | 186 |
| MsG0880046415.01.T01 | AT5G51570 | 59.664 | 119 | 48 | 0 | 21 | 139 | 166 | 284 | 1.34e-49 | 160 |
Find 35 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGACACTATGAAAGAAAT+TGG | 0.235356 | 8:-73170525 | MsG0880046415.01.T01:CDS |
| GGAGAAGTCCGTCACGAATT+TGG | 0.259803 | 8:+73170436 | None:intergenic |
| GAACAAAATAGATAGATATT+TGG | 0.298939 | 8:+73170819 | None:intergenic |
| GAAACCAGTATAAGAAAATA+TGG | 0.330478 | 8:+73170788 | None:intergenic |
| AATGAACACAGCAGAAGATT+TGG | 0.365320 | 8:+73170494 | None:intergenic |
| TGCAAAAGATGTCATGGATT+TGG | 0.389648 | 8:-73170565 | MsG0880046415.01.T01:CDS |
| AATTCGTGACGGACTTCTCC+AGG | 0.405210 | 8:-73170433 | MsG0880046415.01.T01:CDS |
| GGTTTGAGAAACAGTGTGAT+TGG | 0.406441 | 8:-73170615 | MsG0880046415.01.T01:CDS |
| CTGAGTCTAAATATCTCCAT+GGG | 0.528009 | 8:-73170674 | MsG0880046415.01.T01:CDS |
| CAACGTCAAGCCATTGTGGA+TGG | 0.533502 | 8:-73170636 | MsG0880046415.01.T01:CDS |
| GACAAGTGCAAAAGATGTCA+TGG | 0.548649 | 8:-73170571 | MsG0880046415.01.T01:CDS |
| GTGTTCATTCCACACGGACC+TGG | 0.552060 | 8:-73170480 | MsG0880046415.01.T01:CDS |
| ACTACATCGCGAACAGCACC+AGG | 0.554557 | 8:+73170462 | None:intergenic |
| GTCTAAATATCTCCATGGGG+TGG | 0.557451 | 8:-73170670 | MsG0880046415.01.T01:CDS |
| GAACACAGCAGAAGATTTGG+AGG | 0.567527 | 8:+73170497 | None:intergenic |
| GAGAGTGGCAGCTAAAGAAA+AGG | 0.570066 | 8:-73170742 | MsG0880046415.01.T01:CDS |
| AGTAAAGCGAGCTGAGGGTG+AGG | 0.574736 | 8:-73170697 | MsG0880046415.01.T01:CDS |
| GGCAGCTAAAGAAAAGGCGG+AGG | 0.575338 | 8:-73170736 | MsG0880046415.01.T01:CDS |
| TCTAAATATCTCCATGGGGT+GGG | 0.579878 | 8:-73170669 | MsG0880046415.01.T01:CDS |
| GCTGAGTCTAAATATCTCCA+TGG | 0.580913 | 8:-73170675 | MsG0880046415.01.T01:CDS |
| GAAGCTACTCATGAGAACCC+TGG | 0.616440 | 8:+73170415 | None:intergenic |
| ATTCGTGACGGACTTCTCCA+GGG | 0.627782 | 8:-73170432 | MsG0880046415.01.T01:CDS |
| ATCAGCTGCAAGAATGAGAG+TGG | 0.628496 | 8:-73170757 | MsG0880046415.01.T01:CDS |
| TTGCAAGTAAAGCGAGCTGA+GGG | 0.628661 | 8:-73170702 | MsG0880046415.01.T01:CDS |
| CTTGCAAGTAAAGCGAGCTG+AGG | 0.634337 | 8:-73170703 | MsG0880046415.01.T01:CDS |
| AGTGGCAGCTAAAGAAAAGG+CGG | 0.636183 | 8:-73170739 | MsG0880046415.01.T01:CDS |
| CACAGCAGAAGATTTGGAGG+CGG | 0.636213 | 8:+73170500 | None:intergenic |
| TCGTCAACGTCAAGCCATTG+TGG | 0.640251 | 8:-73170640 | MsG0880046415.01.T01:CDS |
| TCTGCTGTGTTCATTCCACA+CGG | 0.654464 | 8:-73170486 | MsG0880046415.01.T01:CDS |
| GACGAGCAATACCCACCCCA+TGG | 0.656560 | 8:+73170658 | None:intergenic |
| GTTTCTCAAACCATCCACAA+TGG | 0.673360 | 8:+73170626 | None:intergenic |
| CGAACAGCACCAGGTCCGTG+TGG | 0.676751 | 8:+73170471 | None:intergenic |
| GTAGTAAGCCAAATTCGTGA+CGG | 0.698713 | 8:-73170444 | MsG0880046415.01.T01:CDS |
| TGTCAAAGTATTGAGTGACA+AGG | 0.713018 | 8:+73170541 | None:intergenic |
| TGAGTCTAAATATCTCCATG+GGG | 0.748301 | 8:-73170673 | MsG0880046415.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAACAAAATAGATAGATATT+TGG | + | Chr8:73170425-73170444 | None:intergenic | 20.0% |
| !!! | ATATCTATCTATTTTGTTCT+TGG | - | Chr8:73170426-73170445 | MsG0880046415.01.T01:CDS | 20.0% |
| ! | GAAACCAGTATAAGAAAATA+TGG | + | Chr8:73170456-73170475 | None:intergenic | 25.0% |
| ! | TTTGACACTATGAAAGAAAT+TGG | - | Chr8:73170716-73170735 | MsG0880046415.01.T01:CDS | 25.0% |
| !! | ATTGCCATATTTTCTTATAC+TGG | - | Chr8:73170449-73170468 | MsG0880046415.01.T01:CDS | 25.0% |
| ! | GATTTTCAGTTAATGTACCA+GGG | - | Chr8:73170648-73170667 | MsG0880046415.01.T01:CDS | 30.0% |
| AATGAACACAGCAGAAGATT+TGG | + | Chr8:73170750-73170769 | None:intergenic | 35.0% | |
| CTGAGTCTAAATATCTCCAT+GGG | - | Chr8:73170567-73170586 | MsG0880046415.01.T01:CDS | 35.0% | |
| TGAGTCTAAATATCTCCATG+GGG | - | Chr8:73170568-73170587 | MsG0880046415.01.T01:CDS | 35.0% | |
| TGCAAAAGATGTCATGGATT+TGG | - | Chr8:73170676-73170695 | MsG0880046415.01.T01:CDS | 35.0% | |
| ! | GGATTTTCAGTTAATGTACC+AGG | - | Chr8:73170647-73170666 | MsG0880046415.01.T01:CDS | 35.0% |
| !! | TGTCAAAGTATTGAGTGACA+AGG | + | Chr8:73170703-73170722 | None:intergenic | 35.0% |
| GACAAGTGCAAAAGATGTCA+TGG | - | Chr8:73170670-73170689 | MsG0880046415.01.T01:CDS | 40.0% | |
| GCTGAGTCTAAATATCTCCA+TGG | - | Chr8:73170566-73170585 | MsG0880046415.01.T01:CDS | 40.0% | |
| GGTTTGAGAAACAGTGTGAT+TGG | - | Chr8:73170626-73170645 | MsG0880046415.01.T01:CDS | 40.0% | |
| GTAGTAAGCCAAATTCGTGA+CGG | - | Chr8:73170797-73170816 | MsG0880046415.01.T01:CDS | 40.0% | |
| GTTTCTCAAACCATCCACAA+TGG | + | Chr8:73170618-73170637 | None:intergenic | 40.0% | |
| TCTAAATATCTCCATGGGGT+GGG | - | Chr8:73170572-73170591 | MsG0880046415.01.T01:CDS | 40.0% | |
| AGTGGCAGCTAAAGAAAAGG+CGG | - | Chr8:73170502-73170521 | MsG0880046415.01.T01:CDS | 45.0% | |
| ATCAGCTGCAAGAATGAGAG+TGG | - | Chr8:73170484-73170503 | MsG0880046415.01.T01:CDS | 45.0% | |
| GAACACAGCAGAAGATTTGG+AGG | + | Chr8:73170747-73170766 | None:intergenic | 45.0% | |
| GAGAGTGGCAGCTAAAGAAA+AGG | - | Chr8:73170499-73170518 | MsG0880046415.01.T01:CDS | 45.0% | |
| GTCTAAATATCTCCATGGGG+TGG | - | Chr8:73170571-73170590 | MsG0880046415.01.T01:CDS | 45.0% | |
| TCTGCTGTGTTCATTCCACA+CGG | - | Chr8:73170755-73170774 | MsG0880046415.01.T01:CDS | 45.0% | |
| TTGCAAGTAAAGCGAGCTGA+GGG | - | Chr8:73170539-73170558 | MsG0880046415.01.T01:CDS | 45.0% | |
| ! | ACATCTTTTGCACTTGTCCC+TGG | + | Chr8:73170668-73170687 | None:intergenic | 45.0% |
| CAACGTCAAGCCATTGTGGA+TGG | - | Chr8:73170605-73170624 | MsG0880046415.01.T01:CDS | 50.0% | |
| CACAGCAGAAGATTTGGAGG+CGG | + | Chr8:73170744-73170763 | None:intergenic | 50.0% | |
| CTTGCAAGTAAAGCGAGCTG+AGG | - | Chr8:73170538-73170557 | MsG0880046415.01.T01:CDS | 50.0% | |
| GGAGAAGTCCGTCACGAATT+TGG | + | Chr8:73170808-73170827 | None:intergenic | 50.0% | |
| TCGTCAACGTCAAGCCATTG+TGG | - | Chr8:73170601-73170620 | MsG0880046415.01.T01:CDS | 50.0% | |
| !! | AATTCGTGACGGACTTCTCC+AGG | - | Chr8:73170808-73170827 | MsG0880046415.01.T01:CDS | 50.0% |
| !! | ATTCGTGACGGACTTCTCCA+GGG | - | Chr8:73170809-73170828 | MsG0880046415.01.T01:CDS | 50.0% |
| ACTACATCGCGAACAGCACC+AGG | + | Chr8:73170782-73170801 | None:intergenic | 55.0% | |
| AGTAAAGCGAGCTGAGGGTG+AGG | - | Chr8:73170544-73170563 | MsG0880046415.01.T01:CDS | 55.0% | |
| GGCAGCTAAAGAAAAGGCGG+AGG | - | Chr8:73170505-73170524 | MsG0880046415.01.T01:CDS | 55.0% | |
| GTGTTCATTCCACACGGACC+TGG | - | Chr8:73170761-73170780 | MsG0880046415.01.T01:CDS | 55.0% | |
| GACGAGCAATACCCACCCCA+TGG | + | Chr8:73170586-73170605 | None:intergenic | 60.0% | |
| CGAACAGCACCAGGTCCGTG+TGG | + | Chr8:73170773-73170792 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 73170419 | 73170844 | 73170419 | ID=MsG0880046415.01;Name=MsG0880046415.01 |
| Chr8 | mRNA | 73170419 | 73170844 | 73170419 | ID=MsG0880046415.01.T01;Parent=MsG0880046415.01;Name=MsG0880046415.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|141 |
| Chr8 | exon | 73170419 | 73170844 | 73170419 | ID=MsG0880046415.01.T01:exon:11596;Parent=MsG0880046415.01.T01 |
| Chr8 | CDS | 73170419 | 73170844 | 73170419 | ID=MsG0880046415.01.T01:cds;Parent=MsG0880046415.01.T01 |
| Gene Sequence |
| Protein sequence |
