AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010244.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010244.01.T01 MTR_2g079670 96.443 253 8 1 1 252 1 253 1.88e-173 478
MsG0280010244.01.T01 MTR_2g079670 98.361 183 3 0 70 252 22 204 1.06e-134 378
MsG0280010244.01.T01 MTR_2g079670 95.288 191 8 1 1 190 1 191 2.01e-122 347
MsG0280010244.01.T01 MTR_2g013140 66.000 250 83 2 1 249 1 249 1.54e-114 328
MsG0280010244.01.T01 MTR_4g088955 60.956 251 96 2 1 249 64 314 4.99e-104 305
MsG0280010244.01.T01 MTR_2g013140 64.398 191 66 2 1 190 1 190 1.30e-78 235
MsG0280010244.01.T01 MTR_1g102680 47.410 251 122 4 1 246 1 246 6.18e-78 236
MsG0280010244.01.T01 MTR_1g041380 47.619 252 120 7 1 246 1 246 2.28e-76 232
MsG0280010244.01.T01 MTR_2g022520 41.558 231 129 4 1 229 1 227 2.03e-55 178
MsG0280010244.01.T01 MTR_4g117050 38.793 232 135 5 1 229 1 228 4.12e-53 172
MsG0280010244.01.T01 MTR_1g041380 45.361 194 96 6 1 190 1 188 3.83e-51 166
MsG0280010244.01.T01 MTR_4g117050 45.062 162 86 2 69 229 24 183 5.99e-47 155
MsG0280010244.01.T01 MTR_1g019625 75.926 54 6 1 112 158 14 67 3.47e-19 79.3
MsG0280010244.01.T01 MTR_7g059340 76.316 38 9 0 113 150 18 55 9.45e-13 62.8
MsG0280010244.01.T02 MTR_2g079670 96.443 253 8 1 1 252 1 253 1.88e-173 478
MsG0280010244.01.T02 MTR_2g079670 98.361 183 3 0 70 252 22 204 1.06e-134 378
MsG0280010244.01.T02 MTR_2g079670 95.288 191 8 1 1 190 1 191 2.01e-122 347
MsG0280010244.01.T02 MTR_2g013140 66.000 250 83 2 1 249 1 249 1.54e-114 328
MsG0280010244.01.T02 MTR_4g088955 60.956 251 96 2 1 249 64 314 4.99e-104 305
MsG0280010244.01.T02 MTR_2g013140 64.398 191 66 2 1 190 1 190 1.30e-78 235
MsG0280010244.01.T02 MTR_1g102680 47.410 251 122 4 1 246 1 246 6.18e-78 236
MsG0280010244.01.T02 MTR_1g041380 47.619 252 120 7 1 246 1 246 2.28e-76 232
MsG0280010244.01.T02 MTR_2g022520 41.558 231 129 4 1 229 1 227 2.03e-55 178
MsG0280010244.01.T02 MTR_4g117050 38.793 232 135 5 1 229 1 228 4.12e-53 172
MsG0280010244.01.T02 MTR_1g041380 45.361 194 96 6 1 190 1 188 3.83e-51 166
MsG0280010244.01.T02 MTR_4g117050 45.062 162 86 2 69 229 24 183 5.99e-47 155
MsG0280010244.01.T02 MTR_1g019625 75.926 54 6 1 112 158 14 67 3.47e-19 79.3
MsG0280010244.01.T02 MTR_7g059340 76.316 38 9 0 113 150 18 55 9.45e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010244.01.T01 AT4G27450 77.823 248 53 2 1 246 1 248 5.04e-142 398
MsG0280010244.01.T01 AT3G15450 68.145 248 76 3 1 246 1 247 6.98e-123 350
MsG0280010244.01.T01 AT3G15450 66.146 192 62 3 1 190 1 191 6.66e-87 257
MsG0280010244.01.T01 AT5G43830 48.810 252 118 5 1 246 1 247 1.29e-78 238
MsG0280010244.01.T01 AT3G15450 63.536 181 60 4 1 179 1 177 3.01e-73 221
MsG0280010244.01.T01 AT3G22850 44.939 247 132 2 1 246 1 244 2.72e-72 221
MsG0280010244.01.T01 AT5G19140 42.424 231 127 4 1 229 1 227 1.54e-56 181
MsG0280010244.01.T01 AT5G19140 50.000 162 78 2 69 229 32 191 6.59e-51 165
MsG0280010244.01.T01 AT5G19140 39.827 231 121 5 1 229 1 215 1.37e-48 160
MsG0280010244.01.T02 AT4G27450 77.823 248 53 2 1 246 1 248 5.04e-142 398
MsG0280010244.01.T02 AT3G15450 68.145 248 76 3 1 246 1 247 6.98e-123 350
MsG0280010244.01.T02 AT3G15450 66.146 192 62 3 1 190 1 191 6.66e-87 257
MsG0280010244.01.T02 AT5G43830 48.810 252 118 5 1 246 1 247 1.29e-78 238
MsG0280010244.01.T02 AT3G15450 63.536 181 60 4 1 179 1 177 3.01e-73 221
MsG0280010244.01.T02 AT3G22850 44.939 247 132 2 1 246 1 244 2.72e-72 221
MsG0280010244.01.T02 AT5G19140 42.424 231 127 4 1 229 1 227 1.54e-56 181
MsG0280010244.01.T02 AT5G19140 50.000 162 78 2 69 229 32 191 6.59e-51 165
MsG0280010244.01.T02 AT5G19140 39.827 231 121 5 1 229 1 215 1.37e-48 160

Find 41 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACATGTCATCCGTATTT+TGG 0.187704 2:-66608873 None:intergenic
TCACTACCTTGTTTCTTAGA+AGG 0.306212 2:-66608584 None:intergenic
CTTGTTTCTTAGAAGGAAAC+AGG 0.339576 2:-66608577 None:intergenic
GATGTGTGGGGCCAACTTTA+AGG 0.380784 2:+66608312 MsG0280010244.01.T01:CDS
TAAGGCAAGGTTGGTGGTGG+TGG 0.390648 2:-66608507 None:intergenic
TTGCTCCCATTCCATCCAAT+TGG 0.404613 2:-66608383 None:intergenic
GGCAAGGTTGGTGGTGGTGG+TGG 0.418571 2:-66608504 None:intergenic
ATCTATTGCCGGCCAAAATA+CGG 0.422825 2:+66608861 MsG0280010244.01.T01:intron
AAGTAAATAAATGAAGAATT+CGG 0.432388 2:-66608757 None:intergenic
TGGTGGTGGTAGGGTTGGCT+AGG 0.434280 2:-66608490 None:intergenic
CACCCTGTAAAATCTATTGC+CGG 0.442085 2:+66608850 MsG0280010244.01.T01:intron
GAACTTATCAACCTTAAAGT+TGG 0.462511 2:-66608323 None:intergenic
GTGAATCCAATTGGATGGAA+TGG 0.469235 2:+66608377 MsG0280010244.01.T01:CDS
ACGACAAATTCAAAAGGATA+AGG 0.473678 2:-66608525 None:intergenic
TCAAAAGGATAAGGCAAGGT+TGG 0.500770 2:-66608516 None:intergenic
ACTCCTTCACTGTCTATCCT+TGG 0.503821 2:-66608289 None:intergenic
TGAATCCAATTGGATGGAAT+GGG 0.508806 2:+66608378 MsG0280010244.01.T01:CDS
GGATAAGGCAAGGTTGGTGG+TGG 0.509745 2:-66608510 None:intergenic
TGAATCACGACAAATTCAAA+AGG 0.520647 2:-66608531 None:intergenic
GCATCCACTGAACAAGTTGA+AGG 0.521756 2:+66608261 MsG0280010244.01.T01:CDS
AGTTATGCTCATAAAGATGT+TGG 0.538313 2:-66608703 None:intergenic
TGGTTATCGTGGAATATTGA+GGG 0.542024 2:-66608829 None:intergenic
TGTATGTTTCATAGTGAAGG+AGG 0.544867 2:+66608223 MsG0280010244.01.T01:CDS
CTGTTTCCTTCTAAGAAACA+AGG 0.552626 2:+66608578 MsG0280010244.01.T01:three_prime_UTR
GAGTTGGTAGTGAATCCAAT+TGG 0.553165 2:+66608368 MsG0280010244.01.T01:CDS
TGGTAGTGAATCCAATTGGA+TGG 0.564629 2:+66608372 MsG0280010244.01.T01:CDS
GTCAACAGTATTCCTCGAGT+TGG 0.573016 2:+66608352 MsG0280010244.01.T01:CDS
AAAGGATAAGGCAAGGTTGG+TGG 0.574788 2:-66608513 None:intergenic
GTGGTTATCGTGGAATATTG+AGG 0.575948 2:-66608830 None:intergenic
GTTGGAAGTATAAATAACCT+TGG 0.585790 2:-66608685 None:intergenic
GGGTGTATGTTTCATAGTGA+AGG 0.585970 2:+66608220 MsG0280010244.01.T01:intron
ATTGCCTTCAACTTGTTCAG+TGG 0.600704 2:-66608265 None:intergenic
AAAATACTTAACATAGCGGA+GGG 0.614941 2:-66608985 None:intergenic
GACAGTGAAGGAGTGATGTG+TGG 0.622107 2:+66608298 MsG0280010244.01.T01:CDS
ACAGTGAAGGAGTGATGTGT+GGG 0.629204 2:+66608299 MsG0280010244.01.T01:CDS
ACAAGTTGAAGGCAATGCCA+AGG 0.645708 2:+66608272 MsG0280010244.01.T01:CDS
TGTTAAATTAGTTCTCACCA+AGG 0.656284 2:+66608668 MsG0280010244.01.T01:intron
AAATTCAAAAGGATAAGGCA+AGG 0.664416 2:-66608520 None:intergenic
ATGCCAAGGATAGACAGTGA+AGG 0.681425 2:+66608286 MsG0280010244.01.T01:CDS
TTGGATTCACTACCAACTCG+AGG 0.697059 2:-66608364 None:intergenic
CAGTGAAGGAGTGATGTGTG+GGG 0.800044 2:+66608300 MsG0280010244.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGCATTTAATTAAATTT+GGG - Chr2:66609306-66609325 None:intergenic 10.0%
!! AGTAACAAAAAAAAAAAAAA+AGG - Chr2:66606987-66607006 None:intergenic 10.0%
!! ATATTAAAAAAAACAAAAGA+AGG - Chr2:66609539-66609558 None:intergenic 10.0%
!! TAATTGCATTTAATTAAATT+TGG - Chr2:66609307-66609326 None:intergenic 10.0%
!! TCAAATTAAAAATCTAATAA+AGG - Chr2:66609375-66609394 None:intergenic 10.0%
!!! TATTACTTAATTCTATTTTA+AGG - Chr2:66609160-66609179 None:intergenic 10.0%
!!! TATTTTTTAAACTCTAAATA+CGG - Chr2:66608049-66608068 None:intergenic 10.0%
!! AAAAAAATTCTTATCTATTG+TGG - Chr2:66609404-66609423 None:intergenic 15.0%
!! AAGTAAATAAATGAAGAATT+CGG - Chr2:66608760-66608779 None:intergenic 15.0%
!! CAATAAAGGTAAATAAAAAA+AGG - Chr2:66609338-66609357 None:intergenic 15.0%
!! GTAAATACATAAAAACTTTA+CGG + Chr2:66608420-66608439 MsG0280010244.01.T01:three_prime_UTR 15.0%
!! TAGAATTAAGTAATACATTT+AGG + Chr2:66609165-66609184 MsG0280010244.01.T01:intron 15.0%
!!! ATTTTGTTTTCAAGATAAAT+GGG - Chr2:66607861-66607880 None:intergenic 15.0%
!!! TATTTTTTTCGAAAGAAAAA+AGG - Chr2:66609259-66609278 None:intergenic 15.0%
!!! TGATACTTAATTAATTTTCA+AGG + Chr2:66609558-66609577 MsG0280010244.01.T01:intron 15.0%
!!! TTCTTCATTTATTTACTTTT+AGG + Chr2:66608762-66608781 MsG0280010244.01.T01:intron 15.0%
!! AAAACAAAATCTCTTCAAAT+TGG + Chr2:66607871-66607890 MsG0280010244.01.T01:intron 20.0%
!! ACTAAAAATACTTAACATAG+CGG - Chr2:66608992-66609011 None:intergenic 20.0%
!! ATAAAAACTTAATATGGTGT+AGG + Chr2:66607150-66607169 MsG0280010244.01.T01:intron 20.0%
!! ATTTCACAATAGTTCTTTAT+TGG + Chr2:66609777-66609796 MsG0280010244.01.T01:intron 20.0%
!! TTTGTTAGATCAATATGATA+CGG + Chr2:66608008-66608027 MsG0280010244.01.T01:intron 20.0%
!!! ACCATATTAAGTTTTTATGT+CGG - Chr2:66607148-66607167 None:intergenic 20.0%
!!! CATCTTTAATTCCTTTTATA+AGG + Chr2:66607111-66607130 MsG0280010244.01.T01:intron 20.0%
!!! GATTTTGTTTTCAAGATAAA+TGG - Chr2:66607862-66607881 None:intergenic 20.0%
!!! TTCATTTATTTACTTTTAGG+AGG + Chr2:66608765-66608784 MsG0280010244.01.T01:intron 20.0%
!!! TTTTAAAATCATTGAATGCA+GGG + Chr2:66608200-66608219 MsG0280010244.01.T01:intron 20.0%
!!! TTTTTAAAATCATTGAATGC+AGG + Chr2:66608199-66608218 MsG0280010244.01.T01:intron 20.0%
! AATACTTGTTCTATGAACTT+TGG + Chr2:66606858-66606877 MsG0280010244.01.T01:CDS 25.0%
! ACAGAATTCATCCTTATAAA+AGG - Chr2:66607125-66607144 None:intergenic 25.0%
! ACTACTTATTACACATTCAA+AGG - Chr2:66609732-66609751 None:intergenic 25.0%
! AGAATTGTTAACAAAGAACA+TGG - Chr2:66607645-66607664 None:intergenic 25.0%
! ATATCTCATTCACACAATAA+AGG - Chr2:66609352-66609371 None:intergenic 25.0%
! CTACTTATTACACATTCAAA+GGG - Chr2:66609731-66609750 None:intergenic 25.0%
! TAAAAACCTTCTATGTAAGA+AGG + Chr2:66609080-66609099 MsG0280010244.01.T01:intron 25.0%
! TCATAAAGAGATTTCTCATT+TGG - Chr2:66609684-66609703 None:intergenic 25.0%
! TCCGACATAAAAACTTAATA+TGG + Chr2:66607144-66607163 MsG0280010244.01.T01:intron 25.0%
! TTAGACTAAAATAGTTGTTC+CGG + Chr2:66609705-66609724 MsG0280010244.01.T01:intron 25.0%
! TTCATAGAACAAGTATTATC+AGG - Chr2:66606855-66606874 None:intergenic 25.0%
!! TTTTGTAACAACCATGTTAT+TGG - Chr2:66607307-66607326 None:intergenic 25.0%
!!! ATGATGTTTGTTTTTTATGC+AGG + Chr2:66607688-66607707 MsG0280010244.01.T01:intron 25.0%
!!! TGATGTTTGTTTTTTATGCA+GGG + Chr2:66607689-66607708 MsG0280010244.01.T01:intron 25.0%
AAAAATACTTAACATAGCGG+AGG - Chr2:66608989-66609008 None:intergenic 30.0%
AAATTCAAAAGGATAAGGCA+AGG - Chr2:66608523-66608542 None:intergenic 30.0%
AAATTTGGGCGTTAAAGAAA+AGG - Chr2:66609292-66609311 None:intergenic 30.0%
AACCTTTGATGAACAGAAAA+CGG - Chr2:66606918-66606937 None:intergenic 30.0%
AATGATTGTTGGATTGAGAT+CGG - Chr2:66608090-66608109 None:intergenic 30.0%
AATTTGGGCGTTAAAGAAAA+GGG - Chr2:66609291-66609310 None:intergenic 30.0%
ACATATACTGTTTGTTCTTG+GGG + Chr2:66607398-66607417 MsG0280010244.01.T01:CDS 30.0%
ACGACAAATTCAAAAGGATA+AGG - Chr2:66608528-66608547 None:intergenic 30.0%
AGAATTTCTTCAGAAAGCTT+AGG - Chr2:66606810-66606829 None:intergenic 30.0%
AGTACTAATCTCCTTCAATT+TGG - Chr2:66607266-66607285 None:intergenic 30.0%
AGTTATGCTCATAAAGATGT+TGG - Chr2:66608706-66608725 None:intergenic 30.0%
ATAAGCTTCAATCAGAAACA+TGG - Chr2:66607485-66607504 None:intergenic 30.0%
ATTACATCATAACCAACATG+TGG + Chr2:66607898-66607917 MsG0280010244.01.T01:intron 30.0%
ATTTCTCATTTGGTATACCT+TGG - Chr2:66609674-66609693 None:intergenic 30.0%
CACTTCAAACAAATTGTCAA+AGG + Chr2:66608165-66608184 MsG0280010244.01.T01:intron 30.0%
GAACTTATCAACCTTAAAGT+TGG - Chr2:66608326-66608345 None:intergenic 30.0%
GACATATACTGTTTGTTCTT+GGG + Chr2:66607397-66607416 MsG0280010244.01.T01:CDS 30.0%
TAACATAATACACATGTCTC+TGG - Chr2:66607190-66607209 None:intergenic 30.0%
TAGATCAATATGATACGGTT+TGG + Chr2:66608013-66608032 MsG0280010244.01.T01:intron 30.0%
TGAATCACGACAAATTCAAA+AGG - Chr2:66608534-66608553 None:intergenic 30.0%
TGACATATACTGTTTGTTCT+TGG + Chr2:66607396-66607415 MsG0280010244.01.T01:CDS 30.0%
! AAAATACTTAACATAGCGGA+GGG - Chr2:66608988-66609007 None:intergenic 30.0%
! AAGCAATATGGTTTGTCAAA+GGG + Chr2:66607448-66607467 MsG0280010244.01.T01:CDS 30.0%
! GTTGGAAGTATAAATAACCT+TGG - Chr2:66608688-66608707 None:intergenic 30.0%
! TGTTAAATTAGTTCTCACCA+AGG + Chr2:66608668-66608687 MsG0280010244.01.T01:intron 30.0%
! TGTTTCTGATTGAAGCTTAT+AGG + Chr2:66607485-66607504 MsG0280010244.01.T01:CDS 30.0%
!! AAGAAGGCGTTAATACTTAA+TGG + Chr2:66609096-66609115 MsG0280010244.01.T01:intron 30.0%
!! AGAAGGCGTTAATACTTAAT+GGG + Chr2:66609097-66609116 MsG0280010244.01.T01:intron 30.0%
!! AGTTTTGTAGGTTGTTTTGT+GGG + Chr2:66607368-66607387 MsG0280010244.01.T01:intron 30.0%
AAAATGTCATGTGTGACTTC+CGG - Chr2:66609222-66609241 None:intergenic 35.0%
AACCCTCTTGTACAACATTA+AGG - Chr2:66607781-66607800 None:intergenic 35.0%
AAGTGTCTGAGTCTCTAATT+TGG - Chr2:66608150-66608169 None:intergenic 35.0%
AATAGTCCTGCATCATACAA+AGG + Chr2:66606774-66606793 MsG0280010244.01.T01:CDS 35.0%
ACCATATTGCTTGTTCAGTA+AGG - Chr2:66607440-66607459 None:intergenic 35.0%
AGTTCCTTAACAACTTGATC+TGG - Chr2:66607538-66607557 None:intergenic 35.0%
CAAGTTGTTAAGGAACTTGA+TGG + Chr2:66607541-66607560 MsG0280010244.01.T01:CDS 35.0%
CTTAATATGGTGTAGGAGTA+TGG + Chr2:66607157-66607176 MsG0280010244.01.T01:intron 35.0%
GAATCGCAATCATATGTGTT+CGG + Chr2:66607067-66607086 MsG0280010244.01.T01:intron 35.0%
GACCTTAATGTTGTACAAGA+GGG + Chr2:66607776-66607795 MsG0280010244.01.T01:CDS 35.0%
GCTAACACATCAATGATTGT+TGG - Chr2:66608101-66608120 None:intergenic 35.0%
GTTGTTTATGACAGCACATA+TGG + Chr2:66607577-66607596 MsG0280010244.01.T01:CDS 35.0%
TCCTTACTGAACAAGCAATA+TGG + Chr2:66607436-66607455 MsG0280010244.01.T01:CDS 35.0%
TGAATCCAATTGGATGGAAT+GGG + Chr2:66608378-66608397 MsG0280010244.01.T01:CDS 35.0%
TGACCTTAATGTTGTACAAG+AGG + Chr2:66607775-66607794 MsG0280010244.01.T01:CDS 35.0%
TGGTTATCGTGGAATATTGA+GGG - Chr2:66608832-66608851 None:intergenic 35.0%
TGTATGTTTCATAGTGAAGG+AGG + Chr2:66608223-66608242 MsG0280010244.01.T01:CDS 35.0%
TGTGCTGTCATAAACAACAA+AGG - Chr2:66607575-66607594 None:intergenic 35.0%
TTAACGCCTTCTTACATAGA+AGG - Chr2:66609089-66609108 None:intergenic 35.0%
TTATTACACATTCAAAGGGC+CGG - Chr2:66609727-66609746 None:intergenic 35.0%
TTTAAGCCTTTGTATGATGC+AGG - Chr2:66606783-66606802 None:intergenic 35.0%
! ATGTTGGCAATTTTCCACAA+AGG + Chr2:66606726-66606745 MsG0280010244.01.T01:CDS 35.0%
! CAAGCAATATGGTTTGTCAA+AGG + Chr2:66607447-66607466 MsG0280010244.01.T01:CDS 35.0%
! CTGTTTCCTTCTAAGAAACA+AGG + Chr2:66608578-66608597 MsG0280010244.01.T01:three_prime_UTR 35.0%
! CTTGCTAATTTGTGTCCTAA+GGG + Chr2:66609121-66609140 MsG0280010244.01.T01:intron 35.0%
! CTTGTTTCTTAGAAGGAAAC+AGG - Chr2:66608580-66608599 None:intergenic 35.0%
! TACACATGTCATCCGTATTT+TGG - Chr2:66608876-66608895 None:intergenic 35.0%
! TCACTACCTTGTTTCTTAGA+AGG - Chr2:66608587-66608606 None:intergenic 35.0%
! TCTTGCTAATTTGTGTCCTA+AGG + Chr2:66609120-66609139 MsG0280010244.01.T01:intron 35.0%
!! ACAAGGTAGTGAAGTGTTTT+TGG + Chr2:66608595-66608614 MsG0280010244.01.T01:three_prime_UTR 35.0%
!! ATGGTGGATTGAAGTTGTAT+TGG + Chr2:66607717-66607736 MsG0280010244.01.T01:CDS 35.0%
!! CAAGGTAGTGAAGTGTTTTT+GGG + Chr2:66608596-66608615 MsG0280010244.01.T01:three_prime_UTR 35.0%
!! CAGTTTTGTAGGTTGTTTTG+TGG + Chr2:66607367-66607386 MsG0280010244.01.T01:intron 35.0%
!! CTAAGAGATGACAGTTTTGT+AGG + Chr2:66607356-66607375 MsG0280010244.01.T01:intron 35.0%
!! TGGTGGATTGAAGTTGTATT+GGG + Chr2:66607718-66607737 MsG0280010244.01.T01:CDS 35.0%
!!! TTTTTTATGCAGGGTTCTGA+TGG + Chr2:66607698-66607717 MsG0280010244.01.T01:intron 35.0%
ATCTATTGCCGGCCAAAATA+CGG + Chr2:66608861-66608880 MsG0280010244.01.T01:intron 40.0%
CAATTGATACCTGCTGGAAA+AGG - Chr2:66607821-66607840 None:intergenic 40.0%
CACCCTGTAAAATCTATTGC+CGG + Chr2:66608850-66608869 MsG0280010244.01.T01:intron 40.0%
CATCATAACCAACATGTGGT+TGG + Chr2:66607902-66607921 MsG0280010244.01.T01:intron 40.0%
CTCTTATCTAATCAAAGCGC+CGG + Chr2:66609200-66609219 MsG0280010244.01.T01:intron 40.0%
GATGTTGCTGACCAAATTGA+AGG + Chr2:66607252-66607271 MsG0280010244.01.T01:intron 40.0%
GCTAAACAATTGATACCTGC+TGG - Chr2:66607827-66607846 None:intergenic 40.0%
GGGTGTATGTTTCATAGTGA+AGG + Chr2:66608220-66608239 MsG0280010244.01.T01:intron 40.0%
GTGAATCCAATTGGATGGAA+TGG + Chr2:66608377-66608396 MsG0280010244.01.T01:CDS 40.0%
GTGGTTATCGTGGAATATTG+AGG - Chr2:66608833-66608852 None:intergenic 40.0%
GTTTGCTTGAGCCAATAACA+TGG + Chr2:66607293-66607312 MsG0280010244.01.T01:intron 40.0%
TCAAAAGGATAAGGCAAGGT+TGG - Chr2:66608519-66608538 None:intergenic 40.0%
TCCTTAACAACTTGATCTGG+TGG - Chr2:66607535-66607554 None:intergenic 40.0%
TGAACAGAAAACGGCTTATC+AGG - Chr2:66606909-66606928 None:intergenic 40.0%
TGCATGCAATCTAAATCCCT+TGG - Chr2:66607944-66607963 None:intergenic 40.0%
! ACAACTTGATCTGGTGGATA+TGG - Chr2:66607529-66607548 None:intergenic 40.0%
! ATTGCCTTCAACTTGTTCAG+TGG - Chr2:66608268-66608287 None:intergenic 40.0%
! ATTTTACAGGGTGGTTATCG+TGG - Chr2:66608843-66608862 None:intergenic 40.0%
! TCCACCAGATCAAGTTGTTA+AGG + Chr2:66607531-66607550 MsG0280010244.01.T01:CDS 40.0%
! TGGTAGTGAATCCAATTGGA+TGG + Chr2:66608372-66608391 MsG0280010244.01.T01:CDS 40.0%
!! AGCCGTTTTCTGTTCATCAA+AGG + Chr2:66606913-66606932 MsG0280010244.01.T01:intron 40.0%
!! GAGTTGGTAGTGAATCCAAT+TGG + Chr2:66608368-66608387 MsG0280010244.01.T01:CDS 40.0%
!! GGTGGATTGAAGTTGTATTG+GGG + Chr2:66607719-66607738 MsG0280010244.01.T01:CDS 40.0%
!!! TGGAATTGTTTTTGCTGCAC+TGG + Chr2:66607597-66607616 MsG0280010244.01.T01:CDS 40.0%
AAAGGATAAGGCAAGGTTGG+TGG - Chr2:66608516-66608535 None:intergenic 45.0%
AAATCTCACGAACAGCTCCA+AGG + Chr2:66609654-66609673 MsG0280010244.01.T01:intron 45.0%
ACAGTGAAGGAGTGATGTGT+GGG + Chr2:66608299-66608318 MsG0280010244.01.T01:CDS 45.0%
ACTCCTTCACTGTCTATCCT+TGG - Chr2:66608292-66608311 None:intergenic 45.0%
ATGCCAAGGATAGACAGTGA+AGG + Chr2:66608286-66608305 MsG0280010244.01.T01:CDS 45.0%
GGTCATTAACTTCTGCCCTT+AGG - Chr2:66609139-66609158 None:intergenic 45.0%
GTCAACAGTATTCCTCGAGT+TGG + Chr2:66608352-66608371 MsG0280010244.01.T01:CDS 45.0%
TTGCTCCCATTCCATCCAAT+TGG - Chr2:66608386-66608405 None:intergenic 45.0%
TTGGATTCACTACCAACTCG+AGG - Chr2:66608367-66608386 None:intergenic 45.0%
! ACAAGTTGAAGGCAATGCCA+AGG + Chr2:66608272-66608291 MsG0280010244.01.T01:CDS 45.0%
! CGGCAATAGATTTTACAGGG+TGG - Chr2:66608852-66608871 None:intergenic 45.0%
! GCATCCACTGAACAAGTTGA+AGG + Chr2:66608261-66608280 MsG0280010244.01.T01:CDS 45.0%
! GGCCGGCAATAGATTTTACA+GGG - Chr2:66608855-66608874 None:intergenic 45.0%
! TGCGACTCTCATTTTCACGA+GGG + Chr2:66609035-66609054 MsG0280010244.01.T01:intron 45.0%
! TGGCCGGCAATAGATTTTAC+AGG - Chr2:66608856-66608875 None:intergenic 45.0%
! TTTATGCAGGGTTCTGATGG+TGG + Chr2:66607701-66607720 MsG0280010244.01.T01:intron 45.0%
!!! CATGTCATCCGTATTTTGGC+CGG - Chr2:66608872-66608891 None:intergenic 45.0%
ACCTGCTGGAAAAGGAGCAA+AGG - Chr2:66607813-66607832 None:intergenic 50.0%
CAGTGAAGGAGTGATGTGTG+GGG + Chr2:66608300-66608319 MsG0280010244.01.T01:CDS 50.0%
GACAGTGAAGGAGTGATGTG+TGG + Chr2:66608298-66608317 MsG0280010244.01.T01:CDS 50.0%
GATCACAACAGACCCATCAG+CGG - Chr2:66607750-66607769 None:intergenic 50.0%
GATGTGTGGGGCCAACTTTA+AGG + Chr2:66608312-66608331 MsG0280010244.01.T01:CDS 50.0%
GCAGGACTATTCAGCTCTTC+AGG - Chr2:66606765-66606784 None:intergenic 50.0%
GGACTATTCAGCTCTTCAGG+TGG - Chr2:66606762-66606781 None:intergenic 50.0%
GTGGATGAGCAAGACCTTTG+TGG - Chr2:66606743-66606762 None:intergenic 50.0%
TCCGAGTGGTAAGACACCAA+GGG + Chr2:66607925-66607944 MsG0280010244.01.T01:intron 50.0%
! GATCTGGTGGATATGGACCA+CGG - Chr2:66607522-66607541 None:intergenic 50.0%
! GTGCGACTCTCATTTTCACG+AGG + Chr2:66609034-66609053 MsG0280010244.01.T01:intron 50.0%
! TCCTTTGCTCCTTTTCCAGC+AGG + Chr2:66607809-66607828 MsG0280010244.01.T01:CDS 50.0%
!! ATTGGGGTATTGCCGCTGAT+GGG + Chr2:66607735-66607754 MsG0280010244.01.T01:CDS 50.0%
!! TATTGGGGTATTGCCGCTGA+TGG + Chr2:66607734-66607753 MsG0280010244.01.T01:CDS 50.0%
!! TCCCTTGGTGTCTTACCACT+CGG - Chr2:66607929-66607948 None:intergenic 50.0%
CACTCGGACCAACCACATGT+TGG - Chr2:66607913-66607932 None:intergenic 55.0%
GTCCGAGTGGTAAGACACCA+AGG + Chr2:66607924-66607943 MsG0280010244.01.T01:intron 55.0%
GTGGATATGGACCACGGTCA+CGG - Chr2:66607516-66607535 None:intergenic 55.0%
TATAGGACACTCCGTGACCG+TGG + Chr2:66607502-66607521 MsG0280010244.01.T01:CDS 55.0%
! CAACATGTGGTTGGTCCGAG+TGG + Chr2:66607911-66607930 MsG0280010244.01.T01:intron 55.0%
!! GGATAAGGCAAGGTTGGTGG+TGG - Chr2:66608513-66608532 None:intergenic 55.0%
!! TAAGGCAAGGTTGGTGGTGG+TGG - Chr2:66608510-66608529 None:intergenic 55.0%
!! AGGTTGGTGGTGGTGGTGGT+AGG - Chr2:66608503-66608522 None:intergenic 60.0%
!! GGTTGGTGGTGGTGGTGGTA+GGG - Chr2:66608502-66608521 None:intergenic 60.0%
!! TGGTGGTGGTAGGGTTGGCT+AGG - Chr2:66608493-66608512 None:intergenic 60.0%
!! GGCAAGGTTGGTGGTGGTGG+TGG - Chr2:66608507-66608526 None:intergenic 65.0%
!! GGTGGTGGTGGTGGTAGGGT+TGG - Chr2:66608498-66608517 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 66606726 66609847 66606726 ID=MsG0280010244.01;Name=MsG0280010244.01
Chr2 mRNA 66606726 66609847 66606726 ID=MsG0280010244.01.T01;Parent=MsG0280010244.01;Name=MsG0280010244.01.T01;_AED=0.31;_eAED=0.32;_QI=0|0.75|0.6|1|0.5|0.2|5|262|252
Chr2 exon 66608221 66608655 66608221 ID=MsG0280010244.01.T01:exon:14318;Parent=MsG0280010244.01.T01
Chr2 exon 66609830 66609847 66609830 ID=MsG0280010244.01.T01:exon:14319;Parent=MsG0280010244.01.T01
Chr2 CDS 66606726 66606923 66606726 ID=MsG0280010244.01.T01:cds;Parent=MsG0280010244.01.T01
Chr2 CDS 66607370 66607618 66607370 ID=MsG0280010244.01.T01:cds;Parent=MsG0280010244.01.T01
Chr2 CDS 66607710 66607830 66607710 ID=MsG0280010244.01.T01:cds;Parent=MsG0280010244.01.T01
Chr2 CDS 66608221 66608411 66608221 ID=MsG0280010244.01.T01:cds;Parent=MsG0280010244.01.T01
Chr2 three_prime_UTR 66608412 66608655 66608412 ID=MsG0280010244.01.T01:three_prime_utr;Parent=MsG0280010244.01.T01
Chr2 three_prime_UTR 66609830 66609847 66609830 ID=MsG0280010244.01.T01:three_prime_utr;Parent=MsG0280010244.01.T01
Chr2 mRNA 66606726 66609018 66606726 ID=MsG0280010244.01.T02;Parent=MsG0280010244.01;Name=MsG0280010244.01.T02;_AED=0.25;_eAED=0.25;_QI=0|0.66|0.5|1|0.66|0.5|4|607|252
Chr2 exon 66608221 66609018 66608221 ID=MsG0280010244.01.T02:exon:14320;Parent=MsG0280010244.01.T02
Chr2 CDS 66606726 66606923 66606726 ID=MsG0280010244.01.T02:cds;Parent=MsG0280010244.01.T02
Chr2 CDS 66607370 66607618 66607370 ID=MsG0280010244.01.T02:cds;Parent=MsG0280010244.01.T02
Chr2 CDS 66607710 66607830 66607710 ID=MsG0280010244.01.T02:cds;Parent=MsG0280010244.01.T02
Chr2 CDS 66608221 66608411 66608221 ID=MsG0280010244.01.T02:cds;Parent=MsG0280010244.01.T02
Chr2 three_prime_UTR 66608412 66609018 66608412 ID=MsG0280010244.01.T02:three_prime_utr;Parent=MsG0280010244.01.T02
Gene Sequence

>MsG0280010244.01.T01

ATGTTGGCAATTTTCCACAAAGGTCTTGCTCATCCACCTGAAGAGCTGAATAGTCCTGCATCATACAAAGGCTTAAAGAAACCTAAGCTTTCTGAAGAAATTCTCAGAGAATTCATTTCTCATCATCCTGATAATACTTGTTCTATGAACTTTGGAAAAGCTGCTCTTGCTTATGTTAGACCTGATAAGCCGTTTTCTTTTTGTAGGTTGTTTTGTGGGCTTGATGACATATACTGTTTGTTCTTGGGGAGTTTGAACAATTTGTCCTTACTGAACAAGCAATATGGTTTGTCAAAGGGAACTGATGAAGCCATGTTTCTGATTGAAGCTTATAGGACACTCCGTGACCGTGGTCCATATCCACCAGATCAAGTTGTTAAGGAACTTGATGGCAGTTTTGCCTTTGTTGTTTATGACAGCACATATGGAATTGTTTTTGCTGCACTGGGTTCTGATGGTGGATTGAAGTTGTATTGGGGTATTGCCGCTGATGGGTCTGTTGTGATCTCTGATGACCTTAATGTTGTACAAGAGGGTTGTGCTAAATCCTTTGCTCCTTTTCCAGCAGGGTGTATGTTTCATAGTGAAGGAGGTTTAATGAGCTTTGAGCATCCACTGAACAAGTTGAAGGCAATGCCAAGGATAGACAGTGAAGGAGTGATGTGTGGGGCCAACTTTAAGGTTGATAAGTTCTCAAGAGTCAACAGTATTCCTCGAGTTGGTAGTGAATCCAATTGGATGGAATGGGAGCAACATTAG

>MsG0280010244.01.T02

ATGTTGGCAATTTTCCACAAAGGTCTTGCTCATCCACCTGAAGAGCTGAATAGTCCTGCATCATACAAAGGCTTAAAGAAACCTAAGCTTTCTGAAGAAATTCTCAGAGAATTCATTTCTCATCATCCTGATAATACTTGTTCTATGAACTTTGGAAAAGCTGCTCTTGCTTATGTTAGACCTGATAAGCCGTTTTCTTTTTGTAGGTTGTTTTGTGGGCTTGATGACATATACTGTTTGTTCTTGGGGAGTTTGAACAATTTGTCCTTACTGAACAAGCAATATGGTTTGTCAAAGGGAACTGATGAAGCCATGTTTCTGATTGAAGCTTATAGGACACTCCGTGACCGTGGTCCATATCCACCAGATCAAGTTGTTAAGGAACTTGATGGCAGTTTTGCCTTTGTTGTTTATGACAGCACATATGGAATTGTTTTTGCTGCACTGGGTTCTGATGGTGGATTGAAGTTGTATTGGGGTATTGCCGCTGATGGGTCTGTTGTGATCTCTGATGACCTTAATGTTGTACAAGAGGGTTGTGCTAAATCCTTTGCTCCTTTTCCAGCAGGGTGTATGTTTCATAGTGAAGGAGGTTTAATGAGCTTTGAGCATCCACTGAACAAGTTGAAGGCAATGCCAAGGATAGACAGTGAAGGAGTGATGTGTGGGGCCAACTTTAAGGTTGATAAGTTCTCAAGAGTCAACAGTATTCCTCGAGTTGGTAGTGAATCCAATTGGATGGAATGGGAGCAACATTAG
Protein sequence

>MsG0280010244.01.T01

MLAIFHKGLAHPPEELNSPASYKGLKKPKLSEEILREFISHHPDNTCSMNFGKAALAYVRPDKPFSFCRLFCGLDDIYCLFLGSLNNLSLLNKQYGLSKGTDEAMFLIEAYRTLRDRGPYPPDQVVKELDGSFAFVVYDSTYGIVFAALGSDGGLKLYWGIAADGSVVISDDLNVVQEGCAKSFAPFPAGCMFHSEGGLMSFEHPLNKLKAMPRIDSEGVMCGANFKVDKFSRVNSIPRVGSESNWMEWEQH*

>MsG0280010244.01.T02

MLAIFHKGLAHPPEELNSPASYKGLKKPKLSEEILREFISHHPDNTCSMNFGKAALAYVRPDKPFSFCRLFCGLDDIYCLFLGSLNNLSLLNKQYGLSKGTDEAMFLIEAYRTLRDRGPYPPDQVVKELDGSFAFVVYDSTYGIVFAALGSDGGLKLYWGIAADGSVVISDDLNVVQEGCAKSFAPFPAGCMFHSEGGLMSFEHPLNKLKAMPRIDSEGVMCGANFKVDKFSRVNSIPRVGSESNWMEWEQH*