AlfalfaGEDB | MsG0880046515.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046515.01

KEGG: K18211
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046515.01.T01	GAU24647.1	68.098	163	23	3	90	223	1	163	1.15E-61	206

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046515.01.T01	Q9S7P9	47.222	144	63	5	90	223	1	141	2.89E-24	100

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046515.01.T01	A0A2Z6N611	68.098	163	23	3	90	223	1	163	5.51e-62	206

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023067.01	MsG0880046515.01	0.807945	3.943654e-50	2.225763e-47
MsG0880046515.01	MsG0880047207.01	-0.825117	5.583430e-54	5.042973e-51

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046515.01.T01	MTR_4g109310	82.979	141	17	3	90	223	1	141	3.02e-64	201
MsG0880046515.01.T01	MTR_3g023720	55.474	137	58	2	90	223	1	137	5.78e-38	133
MsG0880046515.01.T01	MTR_3g023720	55.474	137	58	2	90	223	1	137	1.32e-37	133
MsG0880046515.01.T01	MTR_8g021950	66.071	56	18	1	169	223	13	68	7.51e-15	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046515.01.T01	AT5G61210	47.222	144	63	5	90	223	1	141	2.95e-25	100
MsG0880046515.01.T01	AT5G61210	47.222	144	63	5	90	223	1	141	2.95e-25	100
MsG0880046515.01.T01	AT1G13890	40.000	105	39	3	120	223	25	106	1.09e-13	68.9
MsG0880046515.01.T01	AT1G13890	40.000	105	39	3	120	223	25	106	1.09e-13	68.9
MsG0880046515.01.T01	AT5G07880	43.182	88	45	2	141	223	10	97	9.98e-11	60.5
MsG0880046515.01.T01	AT5G07880	43.182	88	45	2	141	223	10	97	9.98e-11	60.5

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Find 89 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACATGGCTGTTGACA+TGG	0.533190	8:-74263426	MsG0880046515.01.T01:three_prime_UTR
AAAAGTCTCGGAGAAGAGGG+AGG	0.668590	8:+74265335	None:intergenic
AACAATTGTGAACGGATTGG+TGG	0.522197	8:+74264593	None:intergenic
AACTTGGAGTGAGCGTGTTT+TGG	0.402333	8:+74265435	None:intergenic
AAGAAGACACGTACAATAAC+AGG	0.394267	8:-74264033	MsG0880046515.01.T01:three_prime_UTR
AAGAATAATTTCCGTGATTC+TGG	0.208002	8:-74264677	MsG0880046515.01.T01:CDS
AAGACATAAAGAAGTTTGAA+TGG	0.435867	8:+74263019	None:intergenic
AATAATTTCCGTGATTCTGG+CGG	0.606685	8:-74264674	MsG0880046515.01.T01:CDS
AATGCAGATCAAAGTAAATT+CGG	0.408068	8:+74264961	None:intergenic
AATTATTCTTATACCTGTTC+CGG	0.301104	8:+74264690	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAATAATTTCCGTGATTC+TGG	-	Chr8:74263811-74263830	MsG0880046515.01.T01:three_prime_UTR	30.0%
ACAAGTAAAGTCTTTCTCAA+AGG	+	Chr8:74265180-74265199	None:intergenic	30.0%
ACATCTTGCTTCAATATTAC+AGG	-	Chr8:74263495-74263514	MsG0880046515.01.T01:intron	30.0%
ACATTGATCGAGATTTAAGT+CGG	-	Chr8:74264014-74264033	MsG0880046515.01.T01:intron	30.0%
ATGATGTATCCATTACAGTA+TGG	+	Chr8:74265370-74265389	None:intergenic	30.0%
CAGTAAGTGTAAACTAAAGA+TGG	+	Chr8:74265405-74265424	None:intergenic	30.0%
CATCTTGCTTCAATATTACA+GGG	-	Chr8:74263496-74263515	MsG0880046515.01.T01:intron	30.0%
CTATCTTCAACAATTGTGAA+CGG	+	Chr8:74263906-74263925	None:intergenic	30.0%
GAAAGACTTTACTTGTTCTA+TGG	-	Chr8:74265184-74265203	MsG0880046515.01.T01:intron	30.0%
GAACAAAGAGAATTGGAAAA+TGG	+	Chr8:74263122-74263141	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	74262999	74265511	74262999	ID=MsG0880046515.01;Name=MsG0880046515.01
Chr8	mRNA	74262999	74265511	74262999	ID=MsG0880046515.01.T01;Parent=MsG0880046515.01;Name=MsG0880046515.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0.6\|0.5\|1\|0\|0.16\|6\|640\|226
Chr8	exon	74262999	74263272	74262999	ID=MsG0880046515.01.T01:exon:30921;Parent=MsG0880046515.01.T01
Chr8	exon	74263410	74263507	74263410	ID=MsG0880046515.01.T01:exon:30920;Parent=MsG0880046515.01.T01
Chr8	exon	74263798	74263928	74263798	ID=MsG0880046515.01.T01:exon:30919;Parent=MsG0880046515.01.T01
Chr8	exon	74264016	74264108	74264016	ID=MsG0880046515.01.T01:exon:30918;Parent=MsG0880046515.01.T01
Chr8	exon	74264473	74264993	74264473	ID=MsG0880046515.01.T01:exon:30917;Parent=MsG0880046515.01.T01
Chr8	exon	74265308	74265511	74265308	ID=MsG0880046515.01.T01:exon:30916;Parent=MsG0880046515.01.T01
Chr8	CDS	74265308	74265511	74265308	ID=MsG0880046515.01.T01:cds;Parent=MsG0880046515.01.T01
Chr8	CDS	74264517	74264993	74264517	ID=MsG0880046515.01.T01:cds;Parent=MsG0880046515.01.T01
Chr8	three_prime_UTR	74264473	74264516	74264473	ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8	three_prime_UTR	74264016	74264108	74264016	ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8	three_prime_UTR	74263798	74263928	74263798	ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8	three_prime_UTR	74263410	74263507	74263410	ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8	three_prime_UTR	74262999	74263272	74262999	ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01

Gene Sequence

>MsG0880046515.01.T01

CCTCCTATAGTAATAAACAAACCAAAAAAAAAAGAAAGAAGAGCGCGTGAATCACCAAAACACGCTCACTCCAAGTTCTTCTTTCTTCTTTGCAGTTTCCCAATCGAACATTTGTTGACGCCATTTTCCAATTCTCTTTGTTCTTATTCTAATTCCTCCCTCTTCTCCGAGACTTTTTTTATCGAATCTTTCTGCTTCTTCCAGGGAGTAAAAACCGAATTTACTTTGATCTGCATTTCACTCAATTCTGCAATAACAGAGACGACAATGTTCAATTCAAAGAATTCTCCTGTCAATGATGTTAAATCCTGGTCGAATCCGTTTGATTCTGATGATGAGGAAAAGGATAGCAGAAAGATGAGTTCGTCGAAGAAGACGTCTTCAGAACGAGCACTAGTTACCTTAGAAGTGAACACCACCCCGTTTGATGATGTTGATGACACTAAGAAAACTCCATCATCAACGTATGTTCATCGATCAGCAGACCGGAACAGGTATAAGAATAATTTCCGTGATTCTGGCGGAGTAGAGAATCAATCCGTGCAGGAGTTGGAGAATTATGCTGTTTACAAGCTCAGGAGACCACCAATCCGTTCACAATTGTTGAAGATAGCAGAGAACATAAGAGAGGATGCTGCCAAGACATTGGTCACCCTTCATCACCAGGGAGCAAATTACTAG

Protein sequence

>MsG0880046515.01.T01

PPIVINKPKKKERRARESPKHAHSKFFFLLCSFPIEHLLTPFSNSLCSYSNSSLFSETFFIESFCFFQGVKTEFTLICISLNSAITETTMFNSKNSPVNDVKSWSNPFDSDDEEKDSRKMSSSKKTSSERALVTLEVNTTPFDDVDDTKKTPSSTYVHRSADRNRYKNNFRDSGGVENQSVQELENYAVYKLRRPPIRSQLLKIAENIREDAAKTLVTLHHQGANY*