AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046515.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046515.01.T01 MTR_4g109310 82.979 141 17 3 90 223 1 141 3.02e-64 201
MsG0880046515.01.T01 MTR_3g023720 55.474 137 58 2 90 223 1 137 5.78e-38 133
MsG0880046515.01.T01 MTR_3g023720 55.474 137 58 2 90 223 1 137 1.32e-37 133
MsG0880046515.01.T01 MTR_8g021950 66.071 56 18 1 169 223 13 68 7.51e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046515.01.T01 AT5G61210 47.222 144 63 5 90 223 1 141 2.95e-25 100
MsG0880046515.01.T01 AT5G61210 47.222 144 63 5 90 223 1 141 2.95e-25 100
MsG0880046515.01.T01 AT1G13890 40.000 105 39 3 120 223 25 106 1.09e-13 68.9
MsG0880046515.01.T01 AT1G13890 40.000 105 39 3 120 223 25 106 1.09e-13 68.9
MsG0880046515.01.T01 AT5G07880 43.182 88 45 2 141 223 10 97 9.98e-11 60.5
MsG0880046515.01.T01 AT5G07880 43.182 88 45 2 141 223 10 97 9.98e-11 60.5

Find 89 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTGGTTTCCATGTTT+TGG 0.164104 8:+74264050 None:intergenic
AAGAATAATTTCCGTGATTC+TGG 0.208002 8:-74264677 MsG0880046515.01.T01:CDS
GTCAAATTTAAGTGATCTAT+TGG 0.241177 8:-74263456 MsG0880046515.01.T01:three_prime_UTR
GTCCTGAAACAGTTTGAAAA+AGG 0.245218 8:-74263497 MsG0880046515.01.T01:intron
TCAGCGTGCTCGTCGTCTAA+TGG 0.284873 8:-74263181 MsG0880046515.01.T01:three_prime_UTR
GTTATTGTACGTGTCTTCTT+TGG 0.296263 8:+74264036 None:intergenic
AATTATTCTTATACCTGTTC+CGG 0.301104 8:+74264690 None:intergenic
CGAATCTTTCTGCTTCTTCC+AGG 0.314905 8:-74265307 MsG0880046515.01.T01:intron
CTTCCAGGGAGAAAAGCTTT+TGG 0.334786 8:-74264093 MsG0880046515.01.T01:intron
ACTGTAATGGATACATCATT+TGG 0.335826 8:-74263117 MsG0880046515.01.T01:three_prime_UTR
CGGGGTGGTGTTCACTTCTA+AGG 0.343954 8:+74264775 None:intergenic
CAAAAGGTGAATTTAATCTA+TGG 0.347878 8:+74263057 None:intergenic
GAACAAAGAGAATTGGAAAA+TGG 0.352970 8:+74265369 None:intergenic
TTTGGTTTGTTTATTACTAT+AGG 0.359749 8:+74265486 None:intergenic
CAGCGTGCTCGTCGTCTAAT+GGG 0.360616 8:-74263180 MsG0880046515.01.T01:three_prime_UTR
AGAATAAGAACAAAGAGAAT+TGG 0.380094 8:+74265362 None:intergenic
ATCGATCAGCAGACCGGAAC+AGG 0.386256 8:-74264703 MsG0880046515.01.T01:CDS
AAGAAGACACGTACAATAAC+AGG 0.394267 8:-74264033 MsG0880046515.01.T01:three_prime_UTR
AACTTGGAGTGAGCGTGTTT+TGG 0.402333 8:+74265435 None:intergenic
AATGCAGATCAAAGTAAATT+CGG 0.408068 8:+74264961 None:intergenic
TCAAATTTAAGTGATCTATT+GGG 0.415335 8:-74263455 MsG0880046515.01.T01:three_prime_UTR
ATGATGTATCCATTACAGTA+TGG 0.416951 8:+74263121 None:intergenic
ATTATGCTGTTTACAAGCTC+AGG 0.417804 8:-74264619 MsG0880046515.01.T01:CDS
CTGAGGTGGAGTTCGCAGCC+TGG 0.418844 8:+74263828 None:intergenic
GAGCAGTGACATTGCACAAA+AGG 0.432983 8:+74263041 None:intergenic
AAGACATAAAGAAGTTTGAA+TGG 0.435867 8:+74263019 None:intergenic
GGCGTCAACAAATGTTCGAT+TGG 0.443453 8:+74265390 None:intergenic
CTTGGAACAGAGAGAGAAGT+TGG 0.449507 8:-74263878 MsG0880046515.01.T01:three_prime_UTR
CTGTAATGGATACATCATTT+GGG 0.450805 8:-74263116 MsG0880046515.01.T01:three_prime_UTR
TGATTCTGATGATGAGGAAA+AGG 0.458014 8:-74264852 MsG0880046515.01.T01:CDS
TGTCATCAACATCATCAAAC+GGG 0.460366 8:+74264756 None:intergenic
CTGCATCCGAAGGGCAGTCC+AGG 0.468430 8:-74263846 MsG0880046515.01.T01:three_prime_UTR
TCGCAGCCTGGACTGCCCTT+CGG 0.470997 8:+74263840 None:intergenic
TCCTCATCATCAGAATCAAA+CGG 0.472306 8:+74264857 None:intergenic
GAATCAATCCGTGCAGGAGT+TGG 0.484109 8:-74264645 MsG0880046515.01.T01:CDS
GCGTCAACAAATGTTCGATT+GGG 0.501745 8:+74265391 None:intergenic
GTGTCATCAACATCATCAAA+CGG 0.502705 8:+74264755 None:intergenic
CAGTAAGTGTAAACTAAAGA+TGG 0.511214 8:+74263086 None:intergenic
AGATGATGCGGTCAGAAGAA+AGG 0.512949 8:-74263908 MsG0880046515.01.T01:three_prime_UTR
CAGGATTTAACATCATTGAC+AGG 0.519852 8:+74264887 None:intergenic
AACAATTGTGAACGGATTGG+TGG 0.522197 8:+74264593 None:intergenic
AATTTGCTCCCTGGTGATGA+AGG 0.523518 8:+74264522 None:intergenic
CTGTCAATGATGTTAAATCC+TGG 0.525081 8:-74264886 MsG0880046515.01.T01:CDS
AAAAGACATGGCTGTTGACA+TGG 0.533190 8:-74263426 MsG0880046515.01.T01:three_prime_UTR
TCACCAGGGAGCAAATTACT+AGG 0.534447 8:-74264516 MsG0880046515.01.T01:exon
GAGTTGAATTTCCGAGTGAA+AGG 0.537349 8:-74263210 MsG0880046515.01.T01:three_prime_UTR
TCCGTTTGATTCTGATGATG+AGG 0.540959 8:-74264858 MsG0880046515.01.T01:CDS
CAGAAGAAAGGGAAACCACT+TGG 0.541759 8:-74263896 MsG0880046515.01.T01:three_prime_UTR
GCATCTGTTGGTTCCTGAGG+TGG 0.541871 8:+74263814 None:intergenic
CAGGCTGCGAACTCCACCTC+AGG 0.542135 8:-74263827 MsG0880046515.01.T01:three_prime_UTR
CGATGAACATACGTTGATGA+TGG 0.545998 8:+74264722 None:intergenic
TTGGAACAGAGAGAGAAGTT+GGG 0.548541 8:-74263877 MsG0880046515.01.T01:three_prime_UTR
GATGATGCGGTCAGAAGAAA+GGG 0.550218 8:-74263907 MsG0880046515.01.T01:three_prime_UTR
TTCAACAATTGTGAACGGAT+TGG 0.552937 8:+74264590 None:intergenic
GCAAAGAAGAAAGAAGAACT+TGG 0.555594 8:+74265419 None:intergenic
GGTTTGTTTATTACTATAGG+AGG 0.556480 8:+74265489 None:intergenic
ACATTGATCGAGATTTAAGT+CGG 0.558173 8:-74264474 MsG0880046515.01.T01:three_prime_UTR
ACTCCTAGTAATTTGCTCCC+TGG 0.564792 8:+74264513 None:intergenic
GGATTGACTTCTGCATCCGA+AGG 0.568407 8:-74263856 MsG0880046515.01.T01:three_prime_UTR
CTATCTTCAACAATTGTGAA+CGG 0.569398 8:+74264585 None:intergenic
ATTGGTCACCCTTCATCACC+AGG 0.572367 8:-74264531 MsG0880046515.01.T01:CDS
TCTCTACTCCGCCAGAATCA+CGG 0.572418 8:+74264666 None:intergenic
CATTGATCGAGATTTAAGTC+GGG 0.574898 8:-74264473 MsG0880046515.01.T01:three_prime_UTR
ACTTCTCTCTCTGTTCCAAG+TGG 0.575202 8:+74263881 None:intergenic
ATGTTCATCGATCAGCAGAC+CGG 0.577196 8:-74264709 MsG0880046515.01.T01:CDS
AGAATCAAACGGATTCGACC+AGG 0.580437 8:+74264868 None:intergenic
AGTAGAGAATCAATCCGTGC+AGG 0.582352 8:-74264651 MsG0880046515.01.T01:CDS
GCTGATTAGCACCTTTCACT+CGG 0.583306 8:+74263199 None:intergenic
AGAGGATGCTGCCAAGACAT+TGG 0.589813 8:-74264549 MsG0880046515.01.T01:CDS
GAGGCCTCTTCTCCAAAACA+TGG 0.589915 8:-74264062 MsG0880046515.01.T01:three_prime_UTR
ATGAAGGGTGACCAATGTCT+TGG 0.599124 8:+74264538 None:intergenic
AATAATTTCCGTGATTCTGG+CGG 0.606685 8:-74264674 MsG0880046515.01.T01:CDS
GACATGGATCAGAAATTGAG+AGG 0.612728 8:-74263410 MsG0880046515.01.T01:three_prime_UTR
AGTGCATCTGTTGGTTCCTG+AGG 0.613449 8:+74263811 None:intergenic
GATCAATGTCAGTGGCAACA+TGG 0.619375 8:+74264488 None:intergenic
ATAATTCTCCAACTCCTGCA+CGG 0.621109 8:+74264637 None:intergenic
GATAGCAGAGAACATAAGAG+AGG 0.628193 8:-74264567 MsG0880046515.01.T01:CDS
GATTGACTTCTGCATCCGAA+GGG 0.630700 8:-74263855 MsG0880046515.01.T01:three_prime_UTR
ATCATCGTCTAAATGACTCA+AGG 0.636326 8:+74263239 None:intergenic
GAGTCATTTAGACGATGATG+TGG 0.659325 8:-74263235 MsG0880046515.01.T01:three_prime_UTR
TAAATCTCGATCAATGTCAG+TGG 0.661457 8:+74264480 None:intergenic
ATTGGGAGAGCTAAAAGACA+TGG 0.663652 8:-74263438 MsG0880046515.01.T01:three_prime_UTR
AAAAGTCTCGGAGAAGAGGG+AGG 0.668590 8:+74265335 None:intergenic
AGATGCACTTGCAAAAGTCG+AGG 0.670404 8:-74263797 MsG0880046515.01.T01:intron
ATTTGCTCCCTGGTGATGAA+GGG 0.674450 8:+74264523 None:intergenic
ATCAACATCATCAAACGGGG+TGG 0.699679 8:+74264760 None:intergenic
TTCATGTTATGTAGATGATG+CGG 0.714011 8:-74263920 MsG0880046515.01.T01:intron
GTCATCAACATCATCAAACG+GGG 0.717515 8:+74264757 None:intergenic
TTGGTCACCCTTCATCACCA+GGG 0.735000 8:-74264530 MsG0880046515.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAGAAAAGAAAACATA+TGG + Chr8:74264513-74264532 None:intergenic 15.0%
!!! CTATTTTTTAAAATACTCAT+AGG + Chr8:74264247-74264266 None:intergenic 15.0%
!! AAAGTAAAGAAACACAAAAA+AGG + Chr8:74263325-74263344 None:intergenic 20.0%
!! AAGTAAAGAAACACAAAAAA+GGG + Chr8:74263324-74263343 None:intergenic 20.0%
!! AGAGTTTGCAATATTATTAA+TGG + Chr8:74264896-74264915 None:intergenic 20.0%
!! TCAAATTTAAGTGATCTATT+GGG - Chr8:74265033-74265052 MsG0880046515.01.T01:intron 20.0%
!! TTAAACAAATAACAAAGTGA+TGG - Chr8:74265133-74265152 MsG0880046515.01.T01:intron 20.0%
!!! AATATGATGGAATTATTAAG+GGG + Chr8:74263231-74263250 None:intergenic 20.0%
!!! AGAATATGATGGAATTATTA+AGG + Chr8:74263233-74263252 None:intergenic 20.0%
!!! ATGGTTCTCATAATTTATTT+TGG - Chr8:74265203-74265222 MsG0880046515.01.T01:intron 20.0%
!!! CAAGATAAATTTTTTCACAA+AGG + Chr8:74264105-74264124 None:intergenic 20.0%
!!! GAATATGATGGAATTATTAA+GGG + Chr8:74263232-74263251 None:intergenic 20.0%
!!! GTCAAAATTTAGTCAATTTT+CGG + Chr8:74264361-74264380 None:intergenic 20.0%
!!! TTGAAAGTATAGTAGTATAA+TGG + Chr8:74264828-74264847 None:intergenic 20.0%
!!! TTTGGTTTGTTTATTACTAT+AGG + Chr8:74263005-74263024 None:intergenic 20.0%
! AACACAAGAATAGAATATGA+TGG + Chr8:74263244-74263263 None:intergenic 25.0%
! AAGACATAAAGAAGTTTGAA+TGG + Chr8:74265472-74265491 None:intergenic 25.0%
! AATGCAGATCAAAGTAAATT+CGG + Chr8:74263530-74263549 None:intergenic 25.0%
! AATTATTCTTATACCTGTTC+CGG + Chr8:74263801-74263820 None:intergenic 25.0%
! AGAATAAGAACAAAGAGAAT+TGG + Chr8:74263129-74263148 None:intergenic 25.0%
! AGATTCGATAAAAAAAGTCT+CGG + Chr8:74263168-74263187 None:intergenic 25.0%
! AGTAAAGAAACACAAAAAAG+GGG + Chr8:74263323-74263342 None:intergenic 25.0%
! AGTAACCAACAAAGATTAAA+CGG + Chr8:74264803-74264822 None:intergenic 25.0%
! ATAAAATTGAAAGTTGTACC+TGG + Chr8:74263202-74263221 None:intergenic 25.0%
! ATTAACACTAATATTGTGCA+AGG + Chr8:74264947-74264966 None:intergenic 25.0%
! ATTAACCTCCAAAAATAACA+GGG + Chr8:74264481-74264500 None:intergenic 25.0%
! CAAAAGGTGAATTTAATCTA+TGG + Chr8:74265434-74265453 None:intergenic 25.0%
! GTCAAATTTAAGTGATCTAT+TGG - Chr8:74265032-74265051 MsG0880046515.01.T01:intron 25.0%
! TAAATCCGTTTAATCTTTGT+TGG - Chr8:74264795-74264814 MsG0880046515.01.T01:CDS 25.0%
! TATTAACCTCCAAAAATAAC+AGG + Chr8:74264482-74264501 None:intergenic 25.0%
!! CATATTGACTAAAAAAGGAA+GGG + Chr8:74263397-74263416 None:intergenic 25.0%
!!! ATTGTTTTTCCATACTGTAA+TGG - Chr8:74265358-74265377 MsG0880046515.01.T01:CDS 25.0%
!!! CTTCCTTTTTTAGTCAATAT+GGG - Chr8:74263396-74263415 MsG0880046515.01.T01:intron 25.0%
!!! GCTCTTCTTTCTTTTTTTTT+TGG + Chr8:74263023-74263042 None:intergenic 25.0%
!!! TTTATTTTGGTAAATGTTGC+AGG - Chr8:74265216-74265235 MsG0880046515.01.T01:intron 25.0%
AAGAATAATTTCCGTGATTC+TGG - Chr8:74263811-74263830 MsG0880046515.01.T01:three_prime_UTR 30.0%
ACAAGTAAAGTCTTTCTCAA+AGG + Chr8:74265180-74265199 None:intergenic 30.0%
ACATCTTGCTTCAATATTAC+AGG - Chr8:74263495-74263514 MsG0880046515.01.T01:intron 30.0%
ACATTGATCGAGATTTAAGT+CGG - Chr8:74264014-74264033 MsG0880046515.01.T01:intron 30.0%
ATGATGTATCCATTACAGTA+TGG + Chr8:74265370-74265389 None:intergenic 30.0%
CAGTAAGTGTAAACTAAAGA+TGG + Chr8:74265405-74265424 None:intergenic 30.0%
CATCTTGCTTCAATATTACA+GGG - Chr8:74263496-74263515 MsG0880046515.01.T01:intron 30.0%
CTATCTTCAACAATTGTGAA+CGG + Chr8:74263906-74263925 None:intergenic 30.0%
GAAAGACTTTACTTGTTCTA+TGG - Chr8:74265184-74265203 MsG0880046515.01.T01:intron 30.0%
GAACAAAGAGAATTGGAAAA+TGG + Chr8:74263122-74263141 None:intergenic 30.0%
GATAAAGCAAAATCAGAAGA+GGG + Chr8:74263277-74263296 None:intergenic 30.0%
GTCCACAATATCAATTTGAA+CGG + Chr8:74264155-74264174 None:intergenic 30.0%
TCCACAATATCAATTTGAAC+GGG + Chr8:74264154-74264173 None:intergenic 30.0%
TCCTGAAACAGTTTGAAAAA+GGG - Chr8:74264992-74265011 MsG0880046515.01.T01:intron 30.0%
TGATAAAGCAAAATCAGAAG+AGG + Chr8:74263278-74263297 None:intergenic 30.0%
TTCATGTTATGTAGATGATG+CGG - Chr8:74264568-74264587 MsG0880046515.01.T01:CDS 30.0%
TTGTGCTGTATCATTATCAT+AGG - Chr8:74264535-74264554 MsG0880046515.01.T01:CDS 30.0%
! CCATATTGACTAAAAAAGGA+AGG + Chr8:74263398-74263417 None:intergenic 30.0%
! GAACCCATATTGACTAAAAA+AGG + Chr8:74263402-74263421 None:intergenic 30.0%
! GGTTTGTTTATTACTATAGG+AGG + Chr8:74263002-74263021 None:intergenic 30.0%
!! ACATGAAGAAAGCACTATAT+AGG + Chr8:74264075-74264094 None:intergenic 30.0%
!! ACTGTAATGGATACATCATT+TGG - Chr8:74265371-74265390 MsG0880046515.01.T01:CDS 30.0%
!! CTGTAATGGATACATCATTT+GGG - Chr8:74265372-74265391 MsG0880046515.01.T01:CDS 30.0%
!! GTCATACGTTGATTCATATT+TGG - Chr8:74263449-74263468 MsG0880046515.01.T01:three_prime_UTR 30.0%
!!! CCTTCCTTTTTTAGTCAATA+TGG - Chr8:74263395-74263414 MsG0880046515.01.T01:intron 30.0%
AAAAAAAGTCTCGGAGAAGA+GGG + Chr8:74263159-74263178 None:intergenic 35.0%
AAGAAGACACGTACAATAAC+AGG - Chr8:74264455-74264474 MsG0880046515.01.T01:intron 35.0%
AATAATTTCCGTGATTCTGG+CGG - Chr8:74263814-74263833 MsG0880046515.01.T01:three_prime_UTR 35.0%
ACCCGTTCAAATTGATATTG+TGG - Chr8:74264150-74264169 MsG0880046515.01.T01:intron 35.0%
AGGGCAAAAACAGATAAACT+AGG + Chr8:74263378-74263397 None:intergenic 35.0%
ATCATCGTCTAAATGACTCA+AGG + Chr8:74265252-74265271 None:intergenic 35.0%
CAGGATTTAACATCATTGAC+AGG + Chr8:74263604-74263623 None:intergenic 35.0%
CATTGATCGAGATTTAAGTC+GGG - Chr8:74264015-74264034 MsG0880046515.01.T01:intron 35.0%
CTGTCAATGATGTTAAATCC+TGG - Chr8:74263602-74263621 MsG0880046515.01.T01:intron 35.0%
GCAAAGAAGAAAGAAGAACT+TGG + Chr8:74263072-74263091 None:intergenic 35.0%
GGGCAAAAACAGATAAACTA+GGG + Chr8:74263377-74263396 None:intergenic 35.0%
GTCCTGAAACAGTTTGAAAA+AGG - Chr8:74264991-74265010 MsG0880046515.01.T01:intron 35.0%
GTGTCATCAACATCATCAAA+CGG + Chr8:74263736-74263755 None:intergenic 35.0%
GTTATTGTACGTGTCTTCTT+TGG + Chr8:74264455-74264474 None:intergenic 35.0%
TAAAAAAAGTCTCGGAGAAG+AGG + Chr8:74263160-74263179 None:intergenic 35.0%
TAAATCTCGATCAATGTCAG+TGG + Chr8:74264011-74264030 None:intergenic 35.0%
TAACAAAGTGATGGTCGATT+TGG - Chr8:74265142-74265161 MsG0880046515.01.T01:intron 35.0%
TCCTCATCATCAGAATCAAA+CGG + Chr8:74263634-74263653 None:intergenic 35.0%
TGTATTATTACCTGCTTCCA+GGG - Chr8:74264381-74264400 MsG0880046515.01.T01:intron 35.0%
TGTCATCAACATCATCAAAC+GGG + Chr8:74263735-74263754 None:intergenic 35.0%
TTCAACAATTGTGAACGGAT+TGG + Chr8:74263901-74263920 None:intergenic 35.0%
! ATTATGCTGTTTACAAGCTC+AGG - Chr8:74263869-74263888 MsG0880046515.01.T01:three_prime_UTR 35.0%
! TCTTTTCTCATAAGTGCTTG+TGG + Chr8:74264202-74264221 None:intergenic 35.0%
! TGATTCTGATGATGAGGAAA+AGG - Chr8:74263636-74263655 MsG0880046515.01.T01:intron 35.0%
!! CTTCTTTGGTTTCCATGTTT+TGG + Chr8:74264441-74264460 None:intergenic 35.0%
!!! ATAACAGGCCCTGTTATTTT+TGG - Chr8:74264470-74264489 MsG0880046515.01.T01:intron 35.0%
!!! TCCCTTTTTCAAACTGTTTC+AGG + Chr8:74264996-74265015 None:intergenic 35.0%
AAAAGACATGGCTGTTGACA+TGG - Chr8:74265062-74265081 MsG0880046515.01.T01:intron 40.0%
ATAATTCTCCAACTCCTGCA+CGG + Chr8:74263854-74263873 None:intergenic 40.0%
ATTGGGAGAGCTAAAAGACA+TGG - Chr8:74265050-74265069 MsG0880046515.01.T01:intron 40.0%
CTGTATTATTACCTGCTTCC+AGG - Chr8:74264380-74264399 MsG0880046515.01.T01:intron 40.0%
GACATGGATCAGAAATTGAG+AGG - Chr8:74265078-74265097 MsG0880046515.01.T01:intron 40.0%
GAGTCATTTAGACGATGATG+TGG - Chr8:74265253-74265272 MsG0880046515.01.T01:intron 40.0%
GAGTTGAATTTCCGAGTGAA+AGG - Chr8:74265278-74265297 MsG0880046515.01.T01:intron 40.0%
GATAGCAGAGAACATAAGAG+AGG - Chr8:74263921-74263940 MsG0880046515.01.T01:intron 40.0%
GCGTCAACAAATGTTCGATT+GGG + Chr8:74263100-74263119 None:intergenic 40.0%
GTCATCAACATCATCAAACG+GGG + Chr8:74263734-74263753 None:intergenic 40.0%
TAAAGCTTGTCAAGTTCAGC+AGG + Chr8:74264761-74264780 None:intergenic 40.0%
TTGGAACAGAGAGAGAAGTT+GGG - Chr8:74264611-74264630 MsG0880046515.01.T01:CDS 40.0%
! ACTTTTGCAAGTGCATCTGT+TGG + Chr8:74264689-74264708 None:intergenic 40.0%
! CGATGAACATACGTTGATGA+TGG + Chr8:74263769-74263788 None:intergenic 40.0%
! TCCGTTTGATTCTGATGATG+AGG - Chr8:74263630-74263649 MsG0880046515.01.T01:intron 40.0%
!! AACAATTGTGAACGGATTGG+TGG + Chr8:74263898-74263917 None:intergenic 40.0%
!! AGAAAAGCTTTTGGAAGCCT+TGG - Chr8:74264404-74264423 MsG0880046515.01.T01:intron 40.0%
!!! GTTTCCATGTTTTGGAGAAG+AGG + Chr8:74264433-74264452 None:intergenic 40.0%
ACTTCTCTCTCTGTTCCAAG+TGG + Chr8:74264610-74264629 None:intergenic 45.0%
AGATGATGCGGTCAGAAGAA+AGG - Chr8:74264580-74264599 MsG0880046515.01.T01:CDS 45.0%
AGATGCACTTGCAAAAGTCG+AGG - Chr8:74264691-74264710 MsG0880046515.01.T01:CDS 45.0%
AGTAGAGAATCAATCCGTGC+AGG - Chr8:74263837-74263856 MsG0880046515.01.T01:three_prime_UTR 45.0%
AGTCGAGGTTCGTATCTACT+CGG - Chr8:74264706-74264725 MsG0880046515.01.T01:CDS 45.0%
ATCAACATCATCAAACGGGG+TGG + Chr8:74263731-74263750 None:intergenic 45.0%
ATGAAGGGTGACCAATGTCT+TGG + Chr8:74263953-74263972 None:intergenic 45.0%
ATGTTCATCGATCAGCAGAC+CGG - Chr8:74263779-74263798 MsG0880046515.01.T01:intron 45.0%
CAGAAGAAAGGGAAACCACT+TGG - Chr8:74264592-74264611 MsG0880046515.01.T01:CDS 45.0%
CGAATCTTTCTGCTTCTTCC+AGG - Chr8:74263181-74263200 MsG0880046515.01.T01:three_prime_UTR 45.0%
CTTGGAACAGAGAGAGAAGT+TGG - Chr8:74264610-74264629 MsG0880046515.01.T01:CDS 45.0%
GAGCAGTGACATTGCACAAA+AGG + Chr8:74265450-74265469 None:intergenic 45.0%
GATCAATGTCAGTGGCAACA+TGG + Chr8:74264003-74264022 None:intergenic 45.0%
GATGATGCGGTCAGAAGAAA+GGG - Chr8:74264581-74264600 MsG0880046515.01.T01:CDS 45.0%
GCTGATTAGCACCTTTCACT+CGG + Chr8:74265292-74265311 None:intergenic 45.0%
GGCGTCAACAAATGTTCGAT+TGG + Chr8:74263101-74263120 None:intergenic 45.0%
TCACCAGGGAGCAAATTACT+AGG - Chr8:74263972-74263991 MsG0880046515.01.T01:intron 45.0%
! AACTTGGAGTGAGCGTGTTT+TGG + Chr8:74263056-74263075 None:intergenic 45.0%
! AATTTGCTCCCTGGTGATGA+AGG + Chr8:74263969-74263988 None:intergenic 45.0%
! AGAATCAAACGGATTCGACC+AGG + Chr8:74263623-74263642 None:intergenic 45.0%
! AGTCAATTTTCGGCACAGCT+TGG + Chr8:74264351-74264370 None:intergenic 45.0%
! ATTTGCTCCCTGGTGATGAA+GGG + Chr8:74263968-74263987 None:intergenic 45.0%
! CTTCCAAAAGCTTTTCTCCC+TGG + Chr8:74264401-74264420 None:intergenic 45.0%
! CTTCCAGGGAGAAAAGCTTT+TGG - Chr8:74264395-74264414 MsG0880046515.01.T01:intron 45.0%
! GATTGACTTCTGCATCCGAA+GGG - Chr8:74264633-74264652 MsG0880046515.01.T01:CDS 45.0%
! GTCAATTTTCGGCACAGCTT+GGG + Chr8:74264350-74264369 None:intergenic 45.0%
!! AAAGCTTTTGGAAGCCTTGG+AGG - Chr8:74264407-74264426 MsG0880046515.01.T01:intron 45.0%
!! ACTCCTAGTAATTTGCTCCC+TGG + Chr8:74263978-74263997 None:intergenic 45.0%
!!! ACAGGCCCTGTTATTTTTGG+AGG - Chr8:74264473-74264492 MsG0880046515.01.T01:three_prime_UTR 45.0%
AAAAGTCTCGGAGAAGAGGG+AGG + Chr8:74263156-74263175 None:intergenic 50.0%
AGAGGATGCTGCCAAGACAT+TGG - Chr8:74263939-74263958 MsG0880046515.01.T01:intron 50.0%
ATTGGTCACCCTTCATCACC+AGG - Chr8:74263957-74263976 MsG0880046515.01.T01:intron 50.0%
GAATCAATCCGTGCAGGAGT+TGG - Chr8:74263843-74263862 MsG0880046515.01.T01:three_prime_UTR 50.0%
GAGGCCTCTTCTCCAAAACA+TGG - Chr8:74264426-74264445 MsG0880046515.01.T01:intron 50.0%
TCTCTACTCCGCCAGAATCA+CGG + Chr8:74263825-74263844 None:intergenic 50.0%
TTGGTCACCCTTCATCACCA+GGG - Chr8:74263958-74263977 MsG0880046515.01.T01:intron 50.0%
! AAGCTTTTCTCCCTGGAAGC+AGG + Chr8:74264394-74264413 None:intergenic 50.0%
! GGATTGACTTCTGCATCCGA+AGG - Chr8:74264632-74264651 MsG0880046515.01.T01:CDS 50.0%
!! AGTGCATCTGTTGGTTCCTG+AGG + Chr8:74264680-74264699 None:intergenic 50.0%
!! TTTTGGAGAAGAGGCCTCCA+AGG + Chr8:74264424-74264443 None:intergenic 50.0%
ATCGATCAGCAGACCGGAAC+AGG - Chr8:74263785-74263804 MsG0880046515.01.T01:intron 55.0%
CAGCGTGCTCGTCGTCTAAT+GGG - Chr8:74265308-74265327 MsG0880046515.01.T01:CDS 55.0%
CGGGGTGGTGTTCACTTCTA+AGG + Chr8:74263716-74263735 None:intergenic 55.0%
TCAGCGTGCTCGTCGTCTAA+TGG - Chr8:74265307-74265326 MsG0880046515.01.T01:intron 55.0%
!! GCATCTGTTGGTTCCTGAGG+TGG + Chr8:74264677-74264696 None:intergenic 55.0%
CAGGCTGCGAACTCCACCTC+AGG - Chr8:74264661-74264680 MsG0880046515.01.T01:CDS 65.0%
CTGAGGTGGAGTTCGCAGCC+TGG + Chr8:74264663-74264682 None:intergenic 65.0%
CTGCATCCGAAGGGCAGTCC+AGG - Chr8:74264642-74264661 MsG0880046515.01.T01:CDS 65.0%
TCGCAGCCTGGACTGCCCTT+CGG + Chr8:74264651-74264670 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 74262999 74265511 74262999 ID=MsG0880046515.01;Name=MsG0880046515.01
Chr8 mRNA 74262999 74265511 74262999 ID=MsG0880046515.01.T01;Parent=MsG0880046515.01;Name=MsG0880046515.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.6|0.5|1|0|0.16|6|640|226
Chr8 exon 74262999 74263272 74262999 ID=MsG0880046515.01.T01:exon:30921;Parent=MsG0880046515.01.T01
Chr8 exon 74263410 74263507 74263410 ID=MsG0880046515.01.T01:exon:30920;Parent=MsG0880046515.01.T01
Chr8 exon 74263798 74263928 74263798 ID=MsG0880046515.01.T01:exon:30919;Parent=MsG0880046515.01.T01
Chr8 exon 74264016 74264108 74264016 ID=MsG0880046515.01.T01:exon:30918;Parent=MsG0880046515.01.T01
Chr8 exon 74264473 74264993 74264473 ID=MsG0880046515.01.T01:exon:30917;Parent=MsG0880046515.01.T01
Chr8 exon 74265308 74265511 74265308 ID=MsG0880046515.01.T01:exon:30916;Parent=MsG0880046515.01.T01
Chr8 CDS 74265308 74265511 74265308 ID=MsG0880046515.01.T01:cds;Parent=MsG0880046515.01.T01
Chr8 CDS 74264517 74264993 74264517 ID=MsG0880046515.01.T01:cds;Parent=MsG0880046515.01.T01
Chr8 three_prime_UTR 74264473 74264516 74264473 ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8 three_prime_UTR 74264016 74264108 74264016 ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8 three_prime_UTR 74263798 74263928 74263798 ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8 three_prime_UTR 74263410 74263507 74263410 ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Chr8 three_prime_UTR 74262999 74263272 74262999 ID=MsG0880046515.01.T01:three_prime_utr;Parent=MsG0880046515.01.T01
Gene Sequence

>MsG0880046515.01.T01

CCTCCTATAGTAATAAACAAACCAAAAAAAAAAGAAAGAAGAGCGCGTGAATCACCAAAACACGCTCACTCCAAGTTCTTCTTTCTTCTTTGCAGTTTCCCAATCGAACATTTGTTGACGCCATTTTCCAATTCTCTTTGTTCTTATTCTAATTCCTCCCTCTTCTCCGAGACTTTTTTTATCGAATCTTTCTGCTTCTTCCAGGGAGTAAAAACCGAATTTACTTTGATCTGCATTTCACTCAATTCTGCAATAACAGAGACGACAATGTTCAATTCAAAGAATTCTCCTGTCAATGATGTTAAATCCTGGTCGAATCCGTTTGATTCTGATGATGAGGAAAAGGATAGCAGAAAGATGAGTTCGTCGAAGAAGACGTCTTCAGAACGAGCACTAGTTACCTTAGAAGTGAACACCACCCCGTTTGATGATGTTGATGACACTAAGAAAACTCCATCATCAACGTATGTTCATCGATCAGCAGACCGGAACAGGTATAAGAATAATTTCCGTGATTCTGGCGGAGTAGAGAATCAATCCGTGCAGGAGTTGGAGAATTATGCTGTTTACAAGCTCAGGAGACCACCAATCCGTTCACAATTGTTGAAGATAGCAGAGAACATAAGAGAGGATGCTGCCAAGACATTGGTCACCCTTCATCACCAGGGAGCAAATTACTAG
Protein sequence

>MsG0880046515.01.T01

PPIVINKPKKKERRARESPKHAHSKFFFLLCSFPIEHLLTPFSNSLCSYSNSSLFSETFFIESFCFFQGVKTEFTLICISLNSAITETTMFNSKNSPVNDVKSWSNPFDSDDEEKDSRKMSSSKKTSSERALVTLEVNTTPFDDVDDTKKTPSSTYVHRSADRNRYKNNFRDSGGVENQSVQELENYAVYKLRRPPIRSQLLKIAENIREDAAKTLVTLHHQGANY*