AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023067.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023067.01.T01 MTR_8g086020 96.875 160 5 0 1 160 1 160 1.51e-114 321
MsG0480023067.01.T01 MTR_4g070860 53.147 143 61 3 15 155 14 152 6.94e-45 145
MsG0480023067.01.T01 MTR_1g022495 48.795 166 73 6 1 160 1 160 2.16e-41 136
MsG0480023067.01.T01 MTR_3g117120 50.340 147 67 3 15 160 14 155 7.38e-38 127
MsG0480023067.01.T01 MTR_3g112220 57.692 78 33 0 33 110 2 79 2.36e-25 93.6
MsG0480023067.01.T01 MTR_1g087040 40.141 142 74 5 1 140 1 133 5.84e-22 86.3
MsG0480023067.01.T01 MTR_8g015250 39.000 100 59 1 4 103 45 142 2.48e-15 70.1
MsG0480023067.01.T01 MTR_1g017570 40.426 94 53 1 26 119 51 141 3.86e-14 65.9
MsG0480023067.01.T01 MTR_7g029400 39.080 87 53 0 17 103 66 152 4.69e-14 66.6
MsG0480023067.01.T01 MTR_5g015090 36.364 110 64 2 2 110 32 136 5.01e-14 65.9
MsG0480023067.01.T01 MTR_4g097440 38.095 84 52 0 31 114 71 154 9.52e-14 65.5
MsG0480023067.01.T01 MTR_3g109310 31.132 106 73 0 9 114 100 205 1.89e-11 60.8
MsG0480023067.01.T01 MTR_6g016375 34.000 100 66 0 4 103 59 158 2.96e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023067.01.T01 AT1G75390 50.955 157 71 4 2 154 10 164 1.02e-44 145
MsG0480023067.01.T01 AT4G34590 51.449 138 58 3 31 160 23 159 3.26e-38 128
MsG0480023067.01.T01 AT2G18160 42.405 158 68 4 21 157 15 170 5.93e-33 115
MsG0480023067.01.T01 AT1G75390 53.061 98 44 1 2 99 10 105 3.97e-29 103
MsG0480023067.01.T01 AT3G62420 38.621 145 85 3 11 155 2 142 7.66e-28 101
MsG0480023067.01.T01 AT4G37730 38.235 102 52 3 13 103 171 272 7.52e-14 67.8
MsG0480023067.01.T01 AT3G30530 39.216 102 61 1 3 103 48 149 3.79e-12 61.2
MsG0480023067.01.T01 AT1G13600 35.455 110 66 2 17 126 70 174 3.87e-12 61.6
MsG0480023067.01.T01 AT5G49450 45.122 82 45 0 33 114 14 95 4.36e-12 60.5
MsG0480023067.01.T01 AT5G15830 34.426 122 77 1 13 131 52 173 6.13e-12 61.2
MsG0480023067.01.T01 AT2G04038 37.931 87 53 1 17 103 57 142 2.61e-11 58.9

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGAAAATGCTATTCTT+AGG 0.244925 4:+80376456 MsG0480023067.01.T01:CDS
TTGATCTTCGTGCTGATTCT+CGG 0.312597 4:-80376316 None:intergenic
GTTCAATGATTGTGGATTCT+TGG 0.317045 4:+80376577 MsG0480023067.01.T01:CDS
ATGATTGGTTGTTGACTAAC+AGG 0.346804 4:-80376617 None:intergenic
TGCTATTCTTAGGGTTCAAA+TGG 0.377563 4:+80376466 MsG0480023067.01.T01:CDS
CCTGGTGGAACTTATTCATC+TGG 0.409672 4:+80376197 MsG0480023067.01.T01:CDS
ATCTGAAAATGCTATTCTTA+GGG 0.417867 4:+80376457 MsG0480023067.01.T01:CDS
GATCAATTGTTCAATGATTG+TGG 0.455410 4:+80376569 MsG0480023067.01.T01:CDS
TCTTCGTGCTGATTCTCGGT+TGG 0.456704 4:-80376312 None:intergenic
TGTTAGTCAACAACCAATCA+TGG 0.482930 4:+80376619 MsG0480023067.01.T01:CDS
TGATCAGAAGAAGCCATGAT+TGG 0.513873 4:-80376632 None:intergenic
GAGAATCAGCACGAAGATCA+AGG 0.528035 4:+80376318 MsG0480023067.01.T01:CDS
GACCTTGATATGGCTTCTCC+TGG 0.533330 4:+80376179 None:intergenic
CTTCAAAACTCTGGATCAGA+GGG 0.550324 4:+80376230 MsG0480023067.01.T01:CDS
ACAAGCTCTCTTCAAAACTC+TGG 0.550443 4:+80376221 MsG0480023067.01.T01:CDS
CTTGATATGGCTTCTCCTGG+TGG 0.579058 4:+80376182 None:intergenic
TCTTCAAAACTCTGGATCAG+AGG 0.579120 4:+80376229 MsG0480023067.01.T01:CDS
GGGAGATCATAATAACATGC+AGG 0.579180 4:+80376250 MsG0480023067.01.T01:CDS
AAATGGCTGAACTTAGCCAC+AGG 0.585823 4:+80376483 MsG0480023067.01.T01:CDS
CCAGATGAATAAGTTCCACC+AGG 0.607477 4:-80376197 None:intergenic
ATTGTGGATTCTTGGACACA+TGG 0.610654 4:+80376585 MsG0480023067.01.T01:CDS
ACATAACAGATCAAAAGAAG+AGG 0.628528 4:+80376276 MsG0480023067.01.T01:CDS
GATGAAGAAACAACAACACA+TGG 0.646939 4:+80376340 MsG0480023067.01.T01:CDS
CATTAAGTGATTGCAACCTG+TGG 0.653582 4:-80376499 None:intergenic
TACTCAGATGTACCTGAACG+TGG 0.654069 4:+80376433 MsG0480023067.01.T01:CDS
ATTCAAATAAGCACAAACGT+TGG 0.654444 4:+80376404 MsG0480023067.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCTGAAAATGCTATTCTT+AGG + Chr4:80376456-80376475 MsG0480023067.01.T01:CDS 25.0%
! ATCTGAAAATGCTATTCTTA+GGG + Chr4:80376457-80376476 MsG0480023067.01.T01:CDS 25.0%
ACATAACAGATCAAAAGAAG+AGG + Chr4:80376276-80376295 MsG0480023067.01.T01:CDS 30.0%
ATTCAAATAAGCACAAACGT+TGG + Chr4:80376404-80376423 MsG0480023067.01.T01:CDS 30.0%
GATCAATTGTTCAATGATTG+TGG + Chr4:80376569-80376588 MsG0480023067.01.T01:CDS 30.0%
!!! ATCAAGTAACTCACTTTTTC+AGG + Chr4:80376541-80376560 MsG0480023067.01.T01:CDS 30.0%
!!! CTTTTTTCAGCTGTTCAATT+TGG - Chr4:80376379-80376398 None:intergenic 30.0%
GATGAAGAAACAACAACACA+TGG + Chr4:80376340-80376359 MsG0480023067.01.T01:CDS 35.0%
GTTCAATGATTGTGGATTCT+TGG + Chr4:80376577-80376596 MsG0480023067.01.T01:CDS 35.0%
TGCTATTCTTAGGGTTCAAA+TGG + Chr4:80376466-80376485 MsG0480023067.01.T01:CDS 35.0%
TGTTAGTCAACAACCAATCA+TGG + Chr4:80376619-80376638 MsG0480023067.01.T01:CDS 35.0%
! ATGATTGGTTGTTGACTAAC+AGG - Chr4:80376620-80376639 None:intergenic 35.0%
ACAAGCTCTCTTCAAAACTC+TGG + Chr4:80376221-80376240 MsG0480023067.01.T01:CDS 40.0%
ATTGTGGATTCTTGGACACA+TGG + Chr4:80376585-80376604 MsG0480023067.01.T01:CDS 40.0%
CATTAAGTGATTGCAACCTG+TGG - Chr4:80376502-80376521 None:intergenic 40.0%
CTTCAAAACTCTGGATCAGA+GGG + Chr4:80376230-80376249 MsG0480023067.01.T01:CDS 40.0%
GGGAGATCATAATAACATGC+AGG + Chr4:80376250-80376269 MsG0480023067.01.T01:CDS 40.0%
TCTTCAAAACTCTGGATCAG+AGG + Chr4:80376229-80376248 MsG0480023067.01.T01:CDS 40.0%
TGATCAGAAGAAGCCATGAT+TGG - Chr4:80376635-80376654 None:intergenic 40.0%
! CATTTTCAGATTCCACGTTC+AGG - Chr4:80376448-80376467 None:intergenic 40.0%
!! TTGATCTTCGTGCTGATTCT+CGG - Chr4:80376319-80376338 None:intergenic 40.0%
AAATGGCTGAACTTAGCCAC+AGG + Chr4:80376483-80376502 MsG0480023067.01.T01:CDS 45.0%
CCAGATGAATAAGTTCCACC+AGG - Chr4:80376200-80376219 None:intergenic 45.0%
CCTGGTGGAACTTATTCATC+TGG + Chr4:80376197-80376216 MsG0480023067.01.T01:CDS 45.0%
GAGAATCAGCACGAAGATCA+AGG + Chr4:80376318-80376337 MsG0480023067.01.T01:CDS 45.0%
TACTCAGATGTACCTGAACG+TGG + Chr4:80376433-80376452 MsG0480023067.01.T01:CDS 45.0%
!! TCTTCGTGCTGATTCTCGGT+TGG - Chr4:80376315-80376334 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 80376188 80376670 80376188 ID=MsG0480023067.01;Name=MsG0480023067.01
Chr4 mRNA 80376188 80376670 80376188 ID=MsG0480023067.01.T01;Parent=MsG0480023067.01;Name=MsG0480023067.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|160
Chr4 exon 80376188 80376670 80376188 ID=MsG0480023067.01.T01:exon:32443;Parent=MsG0480023067.01.T01
Chr4 CDS 80376188 80376670 80376188 ID=MsG0480023067.01.T01:cds;Parent=MsG0480023067.01.T01
Gene Sequence

>MsG0480023067.01.T01

ATGGCTTCTCCTGGTGGAACTTATTCATCTGGCACAAGCTCTCTTCAAAACTCTGGATCAGAGGGAGATCATAATAACATGCAGGTGAACATAACAGATCAAAAGAAGAGGAAGAGAATGCAATCCAACCGAGAATCAGCACGAAGATCAAGGATGAAGAAACAACAACACATGGAAGATCTAAGCAACCAAATTGAACAGCTGAAAAAAGAGAACATTCAAATAAGCACAAACGTTGGAGTAACTACTCAGATGTACCTGAACGTGGAATCTGAAAATGCTATTCTTAGGGTTCAAATGGCTGAACTTAGCCACAGGTTGCAATCACTTAATGATATTATTCATTACATTGAATCAAGTAACTCACTTTTTCAGGAGACAGATCAATTGTTCAATGATTGTGGATTCTTGGACACATGGAACACTTTTCCTGTTAGTCAACAACCAATCATGGCTTCTTCTGATCATATGTTAATGTATTGA

Protein sequence

>MsG0480023067.01.T01

MASPGGTYSSGTSSLQNSGSEGDHNNMQVNITDQKKRKRMQSNRESARRSRMKKQQHMEDLSNQIEQLKKENIQISTNVGVTTQMYLNVESENAILRVQMAELSHRLQSLNDIIHYIESSNSLFQETDQLFNDCGFLDTWNTFPVSQQPIMASSDHMLMY*